Spektrofotometri UV (DONE)

8/19/2019 Spektrofotometri UV (DONE)

http://slidepdf.com/reader/full/spektrofotometri-uv-done 1/16

LABORATORIUM ANALITIK INSTRUMENTASI

SEMESTER GENAP TAHUN AJARAN 2013

MODUL : Spektrofotometer UV

PEMBIMBING : Drs. Budi Santoso MT

Oleh :

Kelompok :

N!m! : 1" I##! D$%!&'% A&!&'! S" (121)2)01*

2" M" I+,!l A-l%! A" (121)2)01.

3" N!,%l!h H!/&! P" (121)2)020

)" N!-! A-/'%&! (121)2)021

Kel!/ : 1A

!RO"RAM STUDI DI!LOMA IV TEKNIK KIMIA !RODUKSI BERSI#

JURUSAN TEKNIK KIMIA

POLITEKNIK NEGERI BANDUNG

$%&'

Praktikum : 6 Maret 2013

Penyerahan : 13 Maret

2013



I" JUDUL PRAKTIKUM

Pe&e&'-!& K!! K!e%& Me&-&!k!& Spek'oo'ome'e U

II" TUJUAN PER4OBAAN

!" Me&-&!k!& /pek'oo'ome'e e&!& ,e&!"

," Mem!h!m% p%&/%p !& 5!! k!l%,!/% 67e%%k!/% pe!l!'!& /pek'oo'ome'e U !&

/e8e&%/&9!"

5" Mem,-!' l!-'!& /'!&!"

" Mem,-!' /!mpel e&!& ek/'!k/% !& '!&p! ek/'!k/%"

e" Me&e&'-k!& k!! k!e%& !l!m /!mpel"

III" DASAR TEORI

!" Spek'oo'ome'e U

Spek'oo'ome'e U me-p!k!& /!l!h /!'- me'oe !&!l%/%/ 9!& %l!k-k!&

e&!& p!&8!& elom,!& 100)00 &m !'!- ;.;<2.. kJ6mol" S%&! -l'!7%ole' !'!-

/%&! -&- 'e,!% me&8!% -! 8e&%/ 9!%'-

= Ul'!7%ole' 8!-h

= Ul'!7%ole' ek!'

Ul'!7%ole' 8!-h mem%l%k% e&'!& p!&8!& elom,!& > 10 < 200 &m(

/e!&k!& -l'!7%ole' ek!' mem%l%k% e&'!& p!&8!& elom,!& > 200)00 &m"

P!! /pek'oo'ome'e U ,%!/!&9! me&-&!k!& l!mp- e-'e%-m !'!-

%/e,-' 8-! he!7% h%oe& /e,!!% /-m,e 5!h!9!" De-'e%-m me-p!k!& /!l!h /!'-

%/o'op h%oe& 9!& mem%l%k% 1 po'o& !& 1 &e-'o& p!! %&'%&9!"

?!' 9!& !p!' %!&!l%/%/ me&-&!k!& /pek'oo'ome'e U !!l!h #!'

!l!m ,e&'-k l!-'!& !& #!' 'e/e,-' '%!k '!mp!k ,e$!&!" J%k! #!' 'e/e,-'

,e$!&! m!k! pel- %e!k/%k!& e&!& e!e& 'e'e&'- /eh%&! %h!/%lk!& /-!'-

l!-'!& '%!k ,e$!&!" N!m-& ,%!/!&9! #!' 9!& ,e$!&! le,%h ,!&9!k %!&!l%/%/

me&-&!k!& /pek'oo'ome'e /%&! '!mp!k @/pek'oo'ome'e 7%/%,le"

Se&9!$!/e&9!$! o!&%k /e,!%!& ,e/! '%!k '%!k ,e$!&! /eh%&!

/pek'oo'ome'e U le,%h ,!&9!k %-&!k!& !l!m !&!l%/%/ /e&9!$! o!&%k

kh-/-/&9! !l!m pe&e&'-!& /'-k'- /e&9!$! o!&%k"

L!-'!&l!-'!& '%!k ,e$!&! 9!& %!&!l%/%/ me&-&!k!&

/pek'oo'ome'e U '%!k ,oleh 'e!p!' p!'%kel kolo% !'!-p-& /-/pe&/%" K!e&!

!!&9! p!'%kelp!'%kel kolo% !'!-p-& /-/pe&/% !k!& mempe,e/! !,/o,!&/%(

!k%,!'&9! ,%l! %h-,-&k!& e&!& -m-/ 9!& %'--&k!& !% h-k-m L!m,!e'

Bee ko&/e&'!/% #!' 9!& %!&!l%/%/ m!k%& ,e/! !& !p!,%l! %-&!k!& -&'-k

pe&e&'-!& /'-k'- /-!'- /e&9!$! m!k! p%'! p!! /pek'-m !k!& mele,! !% 9!&

/e/-&-h&9!"



A&!l%/%/ me&-&!k!& /%&! -l'!7%ole' ,%!/!&9! %l!k-k!& me&-&!k!&

-l'!7%ole' ek!'( /e!&k!& !&!l%/%/ me&-&!k!& -l'!7%ole' 8!-h m!k! %&/'-me&

9!& %-&!k!& h!-/ !l!m ke!!!& 7!k-m"

H!l %&% %/e,!,k!& 8%k! %-&!k!& -l'!7%ole' 8!-h m!k! -!! !k!& %k-'

me&9e!p p!&8!& elom,!& 9!& %-&!k!&" Ak,!'&9! ke/!l!h!& 9!& %l!k-k!&

m!k%& !'!l( k!e&! 8%k! -!! %k-' me&9e!p m!k! !,/o,!&/% 9!& %h!/%lk!& !k!&

m!k%& ,e/!( 8%k! h!l %&% %h-,-&k!& e&!& h-k-m L!m,eBee m!k! ko&/e&'!/%

#!' 9!& %!&!l%/%/ le,%h '%&% !% 9!& /eh!-/&9!"

S-m,e: h''p:66$!&%,e/!k"$ope//"5om

h''p:66/%l7!&!&%&!",lo/po'"5om

," K!e%&

K!e%&!( !'!- le,%h pop-le %/e,-' k!e%&( %!l!h /e&9!$! !lk!lo% !&'%&!

,e,e&'-k k%/'!l !& memp-&9!% !/! p!h%'" K!e%& ,eke8! /e,!!% o,!' pe!&/!&

p/%ko!k'% !& %-e'%k %&!&"K!e%& mem%l%k% ,e!' molek-l 1.)"1. e&!& -m-/

k%m%! 4*H10 N*O2 !& pH C". @l!-'!& k!e%& 1 !l!m !%"K%/'!l k!e%& !l!m !%

,e-p! 8!-m8!-m ,e5!h!9!" B%l! '%!k me&!&-& !%( k!e%& meleleh !l!m

/-h- 23)23.o4( !& me&9-,l%m p!! /-h- 9!& e&!h( k!e%& m-!h l!-' !l!m

!% p!&!/( !& klooom( 'e'!p% /e%k%' l!-' !l!m !% %&%& !& !lkohol" K!e%&

,e/%!' /e,!!% ,!/! lem!h !& h!&9! !p!' mem,e&'-k !!m e&!& ,!/! k-!'

@A,!h!m( 2011"

S-m,e: h''p:66$$$"5hem%/'9"oh''p:66%"$%k%pe%!"o



Kafein 100 ppm (larutan induk)

0,5 ml 1 ml 2 ml 3 ml2,5 ml

Labu takar 25 mlHCl 0,1 N in!!a tanda bata"

2 ppm # ppm $ ppm 10 ppm 12 ppm

1% !eru"

2% timban!

100 m! "erbuk

tablet

Labu takar 100 ml

Larutan en&er

rin! den!an kerta" "arin! atman

100 ml ltrat

N in!!a tanda bata"

*en!an ek"trak"i

1% +NaH-NH#H

2% .50 ml ltrat

50 ml ltrat pH /.10

Crn! pi"adiklrmetan 25 ml

1% k&k "etiap 5 menit

2% keluarkan udara den!an membuka kran (tanpa menumpakan larutan)

diulan! 3 kali (den!an ltrat an! "ama tetapi diklrmetan an! berbeda)

5 ml diklrmetan (terpi"a den!an ltrat)

+HCl diklrmetan pH #

Crn! pi"aHCl 0,1 N 25 ml

K&k, keluarkan udara, diulan! 3 kali5 ml HCl 0,1 N men!andun

anpa ek"trak"i

.// ml ltrat

1 ml ltrat

Labu takar 25 mlHCl 0,1N in!!a tanda

25 ml ltrat pen!en&eran 4

.23 ml ltrat pen!en&eran 4


Labu takar 25 mlHCl 0,1N in!!a tanda


I" ALAT DAN BAHAN

!" Al!'

1"Spek'oo'ome'e Sh%m!#- U 100

2" K-7e' k-!/!

3" L!,- '!k! 2; ml @C ,-!h)" L!,- '!k! 100 ml

;" 4oo& p%/!h

C" Mo'! !& pe/'le

" Ke'!/ /!%& $h!'m!&

*" Gel!/ k%m%!

." P%pe' 7ol-me 10 ml

10" Bol! h%/!p

," B!h!&

1" L!-'!& %&-k k!e%& 100 ppm

2" H4l 0(1 N

3" Dekloome'!& ; ml

)" N!OH6NH)OH

" PROSEDUR KERJA

!" Mem,-!' l!-'!& /'!&!

," Mem,-!' /!mpel k!e%&



Larutan blank (HCl 0,1 N)

'pektrftmeter "imad7uku8et


ekan 9ba"e &rr ;1*i"pla 0,000 <


Larutan "ampel $ ppm

'pektrftmeter "imad7uku8et "tart

Kur8a ab"rban&e 8" lamda

9data pr&& ;2

=eak (3)

Nilai lamda mak"imum dan ab"rba

5" Pe&-k-!& /pek'-m @pe&e&'-!& l!m! m!k/%m-m



ekan 1 > t ?L4"ikan nilai lamda mak"imum

'ample &mpartment


'pektrftmeter "imad7u


<ut 7er*i"pla 0,000 <

ku8et


Larutan "ampel 2, #, $, 10, 12 ppm

'pektrftmeter "imad7uku8et "tart

Mun&ul tabel ftmetri

" Pe&-k-!& o'ome'%



I" DATA PENGAMATAN!" Peh%'-&!& 7ol-me -&'-k pe&e&5e!& l!-'!& %&-k me&8!% l!-'!& /'!&!

1" 1 " M1 F 2 " M2

1" 100 ppm F 2; ml " 2 ppm

1F 0(; ml

2" 1"M1 F 2"M2

1" 100 ppm F 2; ml " ) ppm

1 F 1 ml

3" 1"M1 F 2"M2

1" 100 ppm F 2; ml " * ppm

1 F 2 ml

)" 1"M1 F 2"M2

1" 100 ppm F 2; ml " 10 ppm

1 F 2(; ml

;" 1"M1 F 2"M2

1" 100 ppm F 2; ml " 12 ppm

1F 3 ml

," Pe&e&'-!& l!m! m!k/%m-m

P!&8!&

Gelom,!&

@10

3

T!&/m%'!&

P!&8!&

Gelom,!&@10

3

T!&/m%'!&

0,3$ 0,001 0,2/ 0,0$$

Ku8et

Ku8et belakan



0,3 0,001 0,2$ 0,220,36 0 0,2 0,3310,35 0,001 0,26 0,22/0,3# 0,001 0,25 0,1160,33 0 0,2# 0,11#

0,32 0,001 0,23 0,1$30,31 0 0,22 0,2$/0,3 0,005

0%2 0%25 0%3 0%35 0%#

0

0%05

0%1

0%15

0%2

0%25

0%3

0%35

Panjang Gelombang Maksimum

=an@an! >elmban!Mak"imum

Panjang Gelombang

Transmitan

5" Pe&-k-!& o'ome'%

Kn"entra"i(ppm)

<

2 0,#1 0,06

# 0,2$ 0,165

$ 0,235 0,3##

10 0,1/5 0,#3#

12 0,165 0,52

'ampel 0,#/0,30/$

0#



2 # 6 $ 10 12 1#

0%0

0%1

0%2

0%3

0%#

0%5

f(A) B 0%05A . 0%01D B 1

Kurva Kalibrasi

Kur8a kalibra"i

Linear (Kur8a kalibra"i)

Konsentrasi

Absorbansi

F 0(0);0(01)

0(30.*0) F 0(0);0(01)

0(30.*0)0(01)F0(0);

F (1.;C)

D!% k-7!( k%'! !p!'k!& ,!h$! ko&/e&'!/% /!mpel !!l!h (1.;C) ppm

II" PEMBAHASAN

I((a D)ianti A *&$&+$+%&,-

P!k'%k-m k!l% %&% ,e'-8-!& -&'-k me&-k- p!&8!& elom,!& m!k/%m-m !%

/-!'- l!-'!& k!e%& !& me&e&'-k!& ko&/e&'!/% !% /-!'- l!-'!& /!mpel e&!&

me&-&!k!& /pek'oo'ome'%" Spek'oo'ome'% !!l!h pe&-k-!& ko&/e&'!/% l!-'!&

,e!/!k!& $!&! 9!& %/e!p !'!- %'e-/k!&" Al!' 9!& %-&!k!& 9!%'-

/pek'oo'ome'e U @Spek'oo'ome'e Sh%m!#- U100 Ph!m!Spe5 9!&

me&-&!k!& /%&! U /e,!!% /-m,e 5!h!9!&9!" S%&! U 9!& %-&!k!& !!l!h

/%&! U ek!'( k!e&! 8%k! me&-&!k!& /%&! U 8!-h -!! !k!& %k-' me&9e!p

elom,!& 9!& %-&!k!& /eh%&! ko&/e&'!/% #!' 9!& 'e-k- !k!& le,%h '%&% !%

ko&/e&'!/% /e,e&!&9!"



L!-'!& k!e%& me-p!k!& l!-'!& 9!& '%!k ,e$!&!/eh%&! m-!h %!&!l%/%/

&%l!% !,/o,!& !& ko&/e&'!/%&9! p!! /pek'oo'ome'e U" P!! p!k'%k-m k!l% %&%

%-&!k!& l!-'!& k!e%& e&!& ,e,!!% ko&/e&'!/% 9!& 'el!h %e&5ek!& !% l!-'!&

%&-k k!e%& 100 ppm" Ko&/e&'!/% l!-'!& /'!&! 9!& %p!k!% p!! pe5o,!!& k!l% %&%

!!l!h 0 ppm( 2 ppm( ) ppm( * ppm( 10 ppm( !& 12 ppm" L!-'!& /'!&! k!e%& 0 ppm

%-&!k!& /e,!!% ,l!&ko 9!& ,e-&/% /e,!!% pe&e&'- '%'%k &ol ko&/e&'!/% p!!

!%k k!l%,!/%"

P!&8!& elom,!& m!k/%m-m %'e&'-k!& e&!& me&-&!k!& l!-'!& '!&!

k!e%& ko&/e&'!/% * ppm" P!! !l!' /pek'oo'ome'e U %&% !k!& l!&/-& %ke'!h-%

&%l!% !,/o,!& !% /e'%!p p!&8!& elom,!& 9!& %-&!k!& !& !%k !% &%l!%

!,/o,!& 'eh!!p p!&8!& elom,!& ,e/e'! koo%&!'&9!" D!% !%k 'e/e,-' %!p!'

&%l!% p!&8!& elom,!& m!k/%m-m /e,e/! 20 &m

N%l!% p!&8!& elom,!& m!k/%m-m 'e/e,-' %-&!k!& -&'-k me&e&'-k!& &%l!%

!,/o,!& p!! /e'%!p l!-'!& /'!&! k!e%&" D!% pe5o,!& !p!' %ke'!h-% ,!h$!

/em!k%& ,e/! ko&/e&'!/% l!-'!& /'!&! k!e%& m!k! &%l!% !,/o,!&/%&9! 8-! !k!&

/em!k%& ,e/!" H!l 'e/e,-' me&-&8-k!& ,!h$! /em!k%& ,e/! ko&/e&'!% l!-'!& m!k!

5!h!9! 9!& %/e!p oleh l!-'!& /'!&! 8-! /em!k%& ,e/!"

Ke&!%k!& &%l!% ko&/e&'!/% l!-'!& ,e,!&%& l--/ e&!& ke&!%k!& &%l!%

!,/o,!&&9!" H!l 'e/e,-' !p!' %,-k'%k!& e&!& k-7! k!l%,!/% !&'!! ko&/e&'!/% !&

!,/o,!&" K-7! !k!& ,e,e,'-k l%&e! e&!& pe/!m!!& 9 F 0(0); 0(00*" D!%

pe/!m!!& 'e/e,-' !p!' %ke'!h-% ko&/e&'!/% /!mpel 9!& %-8% 9!%'- (1.; !% &%l!%

!,/o,!& /e,e/! 0(30.*)"

P!! p!k'%k-m k!l% %&% 8-! %l!k-k!& pe&-8%!& k!e%& p!! o,!' 9!&

%ek/'!k/% !& '%!k %ek/'!k/%" T-8-!& !% %l!k-k!&&9! ek/'!k/% !!l!h -&'-k

mem%/!hk!& k!e%& !% 5!mp-!&&9! e&!& me&-&!k!& pel!-'" K!m% mel!k-k!&

pe&-8%!& ke%& 9!& '%!k %ek/'!k/%" O,!' %h!l-/k!& 'ele,%h !h-l- kem-%!&

%'%m,!& /e,!&9!k 0(10 !m" Se,-k o,!' 'e/e,-' kem-%!& %l!-'k!& e&!& H4l 0(1

N h%&! 2; ml" Kem-%!& !m,%l 2 ml l!-'!& 'e/e,-' !& e&5ek!& h%&! 2; ml"

Se'el!h %l!k-k!& pe&-k-!& 'eh!!p l!-'!& o,!' 'e/e,-' e&!& me&-&!k!&

/pek'oo'ome'e U( !p!' %/%mp-lk!& ,!h$! p!&8!& elom,!& 9!& 'e-k- oleh

!l!' !!l!h p!&8!& elom,!& p!!/e'!mol" H!l 'e/e,-' k!e&! p!&8!& elom,!& 9!&

'e-k- oleh /pek'oo'ome'e U mele,%h% range@e&'!& !& p!&8!& elom,!&

m!k/%m-m !% l!-'!& /'!&! k!e%&"



P!! pe&-8%!& k!e%& 9!& %ek/'!k/% 8-! 'e8!% h!l 9!& /!m! /epe'% p!!

pe&-8%!& k!e%& 9!& '%!k %ek/'!k/%" L!-'!& h!/%l ek/'!k/% 'e/e,-' 8-! me&-&8-k!&

p!&8!& elom,!& 9!& mele,%h% p!&8!& elom,!& m!k/%m-m !% l!-'!& /'!&!

k!e%&" Seh%&! !p!' %ke'!h-% ,!h$! !l!m l!-'!& h!/%l ek/'!k/% 'e/e,-' 'e!p!'

kompo&e& 9!& k!! kompo&e&&9! le,%h '%&% !%p!! k!! k!e%&"

Ke/!l!h!&ke/!l!h!& 9!& 'e!p!' p!! p!k'%k-m k!l% %&% !p!' %/e,!,k!&

k!e&! h!lh!l ,e%k-' :

• Kek-!&'el%'%!& p!! /!!' me&-k- l!-'!&

• Al!'!l!' 9!& %-&!k!& '%!k ,e/%h !'!- me&!&-& ,!h!& l!%&

• Ke/!l!h!& /!!' mem,-!' l!-'!&

Naura Austina *&$&+$+%$&-

D!l!m pe5o,!!& %&%( %l!k-k!& pe&e&'-!& k!! k!e%& me&-&!k!& %&/'-me&

9!& ,e&!m! /pek'oo'ome'e -l'!7%ole' Sh%m!#-" K!e&! pe5o,!!& %&% %l!k/!&!k!&

-&'-k me&e&'-k!& k!! k!e%&( m!k! l!-'!& /'!&! 9!& %-&!k!& !l!m pe&-k-!&

p-& me-p!k!& l!-'!& /'!&! k!e%& 9!& 'el!h %ke'!h-% ko&/e&'!/%&9! @2( )( *( 10( 12

ppm" Pe&-k-!& me&-&!k!& /pek'oo'ome'e !!l!h e&!& mem!/-kk!& 5!%!&

@'!&p! e&!p!& !'!-p-& /-/pe&/% ke !l!m k-7e' k-!/! 9!& kem-%!& %m!/-kk!& ke

!l!m !l!' /pek'oo'ome'e" 4!! mem!/-kk!& 5!%!& ke k-7e' !!l!h e&!& 'ele,%h

!h-l- me&homoe&%/!/% @mem,%l!/ k-7e' e&!& l!-'!& 9!& !k!& %-k-( po/e/

pem,%l!/!& %l!k-k!& > 2 k!l%" Se'el!h %,%l!/( l!-'!& 9!& !k!& %-k- %m!/-k!&

h%&! '!&! ,!'!/ p!! k-7e'" B!!& l-! k-7e' %l!p e&!& me&-&!k!& '%/- /!mp!%

'%!k 'e!p!' ,-'%!& !% %l-! pem-k!!& k-7e'( 'e!kh% k-7e' %l!p e&!&

me&-&!k!& ke'!/ pem,e/%h le&/! 9!& mem%l%k% /e!' h!l-/ /eh%&! '%!k

me&!k%,!'k!& pem-k!!& l-! !% k-7e' 'eoe/" Se,el-m me&-k- l!-'!& /'!&!(

'ele,%h !h-l- %l!k-k!& /'!&!%/!/% !l!' e&!& mem!/-kk!& ,l!&ko( 9!%'- H4l 0(1 N(

ke !l!m 2 ,-!h k-7e' !& kem-%!& %m!/-kk!& ke!l!m !l!' @/pek'oo'ome'e"

Se'el!h %'-( k-7e' 9!& 'ele'!k % ep!& %!m,%l !& %!&'% %/%&9! e&!& l!-'!&

/'!&! 9!& !k!& %-&!k!& /e,!!% pe&e&'- p!&8!& elom,!& m!k/%m-m" P!!

pe5o,!!& %&%( %-&!k!& l!-'!& /'!&! * ppm kem-%!& %%k-'% e&!& pe&-k-!&

l!-'!& /'!&! ko&/e&'!/% l!%&&9! !% 9!& 'ee&!h /!mp!% 9!& 'e'%&%" L!-'!&

/'!&! /e&%% %,-!' !% l!-'!& %&-k k!e%& 100 ppm !& H4l 0(1 N /e,!!%

pel!-'6pe&e&5e" H4l %-&!k!& e&!& '-8-!& -&'-k mem,-!' k!e%& ,e!! p!!

ke!!!& !/!m( k!e&! ke!!!& !/!m !k!& mem,-!' pe&-k-!& p!&8!& elom,!&

k!e%& me&5!p!% '%'%k m!k/%m-m" P!&8!& elom,!& 9!& m!k/%m-m mem%l%k%

kepek!!& m!k/%m!l k!e&! 'e8!% pe-,!h!& !,/o,!&/% 9!& p!l%& ,e/!" P!! p!&8!&

elom,!& m!k/%m-m p-& ,e&'-k k-7! !,/o,!&/% meme&-h% h-k-m L!m,e'Bee"



Ap!,%l! %l!k-k!& pe&-k-!& -l!&( ke'%k! p!&8!& elom,!& !l!m ke!!!&

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9!& 'el!h %,-!' @e&!& ek/'!k/% !'!-p-& '!&p! e/'!k/%" Oleh k!e&! %'- pe&-k-!&

!& pem,-!'!& k-7! h!-/ ,!%k !& ,e&!"

P!! pe5o,!!& %&%( l!-'!& /!mpel %,-!' !% '!,le' o,!' /!k%' kep!l!" Pem,-!'!&

l!-'!& /!mpel %l!k-k!& e&!& -! 5!! 9!%'- e&!& ek/'!k/% !& '!&p! ek/'!k/%"

Pem,-!'!& l!-'!& /!mpel e&!& ek/'!k/% me&h!/%lk!& ; ml H4l 0(1 N 9!& %-!

me&!&-& k!e%&( !& pem,-!'!& l!-'!& /!mpel '!&p! ek/'!k/% me&h!/%lk!& 2; ml

H4l 0(1 N 9!& %-! me&!&-& k!e%&"

Pe&-k-!& p!&8!& elom,!& m!k/%m-m p!! /pek'oo'ome'% Sh%m!#-

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/!mpel 9!& 'el!h %,-!' '!&p! ek/'!k/% p-& '%!k 'e-k-( !'!- ,e!! % l-! !&e"

Ke/!l!h!& %&% 'e8!% p!! po/e/ ek/'!k/% !k%,!' k!e%& '%!k !l!m ke!!!& !/!m

@pe&-k-!& pH p!! /!!' ek/'!k/% '%!k 'ep!'" Ke/!l!h!& p!! /!mpel 9!& %,-!'

'!&p! ek/'!k/% !!l!h !k%,!' ko&/e&'!/% k!e%& 'el!l- pek!' !& ,e!! % l-! !&e

pem,!5!!&"K!e&! '!k !! k!e%& 9!& 'e-k-( pem,%m,%& mem,e% l!-'!& /!mpel l!%& 9!&

%,-!' oleh pem,%m,%&" P!! pe&-k-!& /!mpel 9!& %,e% oleh pem,%m,%&(

%!p!'k!& /!mpel e&!& !,/o,!&/% 0(2.C A" De&!& me&-&!k!& k-7!( %!p!'k!&

ko&/e&'!/% /!mpel 9!& %,e% pem,%m,%& !!l!h /e,e/! (1.C ppm"

Mu/ammad I01a2 A A *&$&+$+%&3-

P!k'%k-m %&% ,e'-8-!& -&'-k me&e&'-k!& p!&8!& elom,!& m!k/%m-m !% /-!'-

l!-'!& k!e%& !& me&e&'-k!& ko&/e&'!/% l!-'!& /!mpel e&!& me&-&!k!& p%&/%p

/pek'oo'ome'% !& h-k-m L!m,e'Bee" Al!' 9!& %-&!k!& -&'-k p!k'%k-m !!l!h

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P!! pe5o,!!& %&%( %l!k-k!& pe&e&'-!& k!! k!e%& e&!&

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!% k!e%& e&!& ,e,!!% ko&/e&'!/%( !!l!h 2( )( *( 10( !& 12 ppm( !& !,/o,!&/%



!% l!-'!& 'e/e,-' %-k- /pek'oo'ome'e U" Se,el-m %l!k-k!& pe&-k-!&

/e!p!&( m!k! m!/%&m!/%& kompo&e& h!-/ %'e&'-k!& p!&8!& elom,!&

m!k/%m-m&9! 'ele,%h !h-l- e&!& pe&-k-!& /pek'-m( m-l!% !% l!-'!& * ppm(

!& %%k-'% l!-'!& e&!& ko&/e&'!/% l!%&&9!" Al!/!& pe&-&!!& p!&8!& elom,!&

m!k/%m-m @ m!k/ 9!k&% p!&8!& elom,!& m!k/%m-m mem%l%k% kepek!!& m!k/%m!l

k!e&! 'e8!% pe-,!h!& !,/o,!&/% 9!& p!l%& ,e/! /e'! p!! p!&8!& elom,!&

m!k/%m-m ,e&'-k k-7! !,/o,!&/% meme&-h% h-k-m L!m,e'Bee" D!% pe5o,!!& %&%(

%peoleh p!&8!& elom,!& m!k/%m-m -&'-k k!e%& !!l!h 22(2 &m"

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o'ome'%" D!% pe&-k-!& o'ome'%( !p!' %peoleh k-7! k!l%,!/% !,/o,!&/% 'eh!!p

ko&/e&'!/%" Ko&/e&'!/% l!-'!& /!mpel !p!' %5!% /e'el!h !,/o,!&/% l!-'!& /!mpel

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/!l%& ,e5!mp-" Tek&%k ek/'!k/% /!&!' ,e-&! -&'-k pem%/!h!& /e5!! 5ep!' !&

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9!& %-&!k!& ,e-p! 5oo& p%/!h @p!l%& /eeh!&!( !l!' ek/'!k/% /ohle'( /!mp!%

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-&'-k mem%/!hk!& !& %/ol!/% ,!h!&,!h!& !% 5!mp-!&&9! 9!& 'e8!% % !l!m( -&'-k

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/-!'- #!' ,e!/!k!& pe,!&%&!& %/'%,-/% #!' 9!& 'el!-' !l!m -! pel!-' 9!&

'%!k /!l%& mel!-'k!&" 4!! mel!k-k!& ek/'!k/% 8!&k! pe&ek @pe&o5ok!&

me&-&!k!& 5oo& p%/!h: Se&9!$! 5!% 9!& !k!& %ek/'!k/% %m!/-k!& ke !l!m

5oo& p%/!h( %'!m,!hk!& ke !l!m&9! e'e /e5-k-p&9!( %ko5ok k-!'k-!' -&'-k

mem-!hk!& me&!%k /e&9!$! 'e/e,-' !% pel!-' !%" D%!mk!& /e,e&'! /!mp!% 'e8!%

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p%/!h( l!p%/!& 9!& ,!$!h !k!& me&!l% ke ,!$!h( %'!mp-& !l!m /-!'- $!!h"

Se!&k!& l!p%/!& !'!/ %,%!k!& 'e'%&!l !l!m 5oo& p%/!h"



N!m-&( l!-'!& h!/%l ek/'!k/% 'e/e,-' me&h!/%lk!& &%l!% p!&8!& elom,!& 9!&

mele,%h% p!&8!& elom,!& m!k/%m-m !% l!-'!& /'!&! k!e%& /eh%&! %ke'!h-%

p!! l!-'!& h!/%l ek/'!k/% 'e/e,-' '%!k me&!&-& k!e%&" A!p-& !k'o ke/!l!h!&

9!& mem-&k%&k!& 'e8!% p!! p!k'%k-m %&% !!l!h !!&9! ke/!l!h!& !l!m

pe&emp!'!& /!mpel( ,%/! 8-! k!e&! kek-!&'el%'%!& !l!m mel!k-k!& pe&e&5e!&

/!mpel" A'!-p-& k!e&! !l!' !& ,!h!& k-!& /'e%l !& 'el!h 'eko&'!m%&!/%"

K!e&! ek/'!k/% '%!k ,eh!/%l( m!k! %l!k-k!& pe5o,!!& e&!& /!mpel l!%& 9!&

me&!,-&k!& l!-'!& e /e5!! !5!k" Se'el!h pe/!m!!& !%/ %peoleh m!k!

ko&/e&'!/% k!e%& !l!m /!mpel !p!' %peoleh" Pe&-k-!& ko&/e&'!/% /!mpel

,e!/!k!& h-k-m L!m,e'Bee 9!& me&9!'!k!& h-,-&!& l%&%e%'!/ !&'!! !,/o,!&

e&!& ko&/e&'!/% l!-'!& !&!l%' !& ,e,!&%& 'e,!l%k e&!& '!&/m%'!&" D!l!m

pe5o,!!& %peoleh !,/o,!&/% /e,e/! 0(30.*0 A" D!& h!/%l peh%'-&!& ko&/e&'!/%

/!mpel 9!& /-!h %e&5ek!& !!l!h (1.C ppm"

Na1i2a/ #asna ! *&$&+$+%$&-

Ie&'%%k!/% me&-&!k!& /pek'oo'ome'e U %l!k-k!& e&!& me&-k-

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pel!-'" Bl!&ko 9!& %-&!k!& p!! pe5o,!!& %&% !!l!h H4L 0(1 N"

L!-'!& /'!&! 9!& %-&!k!& !l!m pe&-k-!& p-& me-p!k!& l!-'!&

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pel!-'6pe&e&5e" H4l %-&!k!& e&!& '-8-!& -&'-k mem,-!' k!e%& m!/-k ke !l!m

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elom,!& m!k/%m-m" P!&8!& elom,!& m!k/%m-m %&% 9!& ,e%k-'&9! !k!&

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'eoe/&9! k-7e' 9!& !k!& ,epe&!-h p!! pe&9e!p!& 5!h!9!"

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m!k/%m-m" Pe&e&'-!& p!&8!& elom,!& m!k/%m-m %l!k-k!& e&!& 5!! k-7e'

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'e&!h( % pe5o,!!& %&% 9!%'- * ppm"

Spek'oo'ome'% o'om!'%/ !k!& me&-k- p!&8!& elom,!& m!k/%m-m" H!l

%&% 9!& mem,e!k!& /pek'oo'ome'% U e&!& 7%/%,le 9!%'- pe&-k-!& p!&8!&



elom,!& m!k/%m-m '%!k pel- %l!k-k!& e&!& 5!! pe&-k-!& l!-'!& /e5!!

,e!&'%!&( !& me&!&'% p!&8!& elom,!& /e5!! ,ek!l!"

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ko&/e&'!/% !% k!e%& !& p!&8!& elom,!& m!k/%m-m k!e%&"

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/e,el-m&9!( %!p!'k!& ko&/e&'!/% /!mpel 9!& %,e% pem,%m,%& !!l!h /e,e/!

(1.C ppm"

Ke/!l!h!& ke/!l!h!& 9!& 'e8!% p!! p!k'%k-m %/e,!,k!& k!e&!:

• Pem,-!'!& l!-'!& /!mpel !% o,!' %l!k-k!& k-!& 'el%'%" Ke'el%'%!&

ko&/e&'!/% !m!' mempe&!-h% pe&-k-!& p!! /pek'oo'ome'%

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po/e/ pem,e&'-k!& /!mpel l!-'!& e&!& k!! ko&/e&'!/% /pe/%%k"

"

III" SIMPULAN

Spek'oo'ome'% me-p!k!& me'o! !l!m k%m%! !&!l%/%/ 9!& %-&!k!& -&'-k

me&e&'-k!& kompo/%/% /-!'- #!'( ,!%k /e5!! k-!&'%'!'% m!-p-& k-!l%'!'%" Al!' 9!&

%-&!k!& -&'-k me&-k- /pek'oo'ome'% !!l!h /pek'oo'ome'e" Spek'oo'ome'e

!! ,em!5!mm!5!m( /!l!h /!'-&9! /pek'oo'ome'e U" Spek'oo'ome'e %&%

me&-&!k!& %&'e!k/% !&'!! !%!/% elek'om!&e'%k !& molek-l !'!- !'om !% /-!'-

#!' k%m%!" J!&k!-!& p!&8!& elom,!& -&'-k -l'!7%ole' !!l!h 1.03*0 &m"S%&!

-l'!7%ole' /e&%% 'e,!% me&8!% 2 8e&%/ 9!%'- -l'!7%ole' 8!-h !& -l'!7%ole' ek!'"

P!! pe5o,!!& %&% %/e%!k!& 'ele,%h !h-l- l!-'!& /'!&! k!e%& 9!& 'el!h

%e&5ek!& oleh H4l 0(1 N 9!& ,eko&/e&'!/% 2( )( *( 10( 12 ppm" L!-'!& /'!&! 9!&

%-&!k!& -&'-k me&e&'-k!& p!&8!& elom,!& m!k/%m-m !!l!h l!-'!& /'!&! *

ppm"

J!%( p!! pe5o,!!& %&% %5!% p!&8!& elom,!& 9!& 'ep!'" P!&8!& elom,!&

m!k/%m!l 9!& %!p!' !% pe&-k-!& l!-'!& /'!&! * ppm !!l!h 22(2 &m" P!&8!&



elom,!& m!k/%m-m %&% /el!&8-'&9! %-&!k!& -&'-k mem,-!' k-7! k!l%,!/%" K-7!

k!l%,!/% /e&%% !k!& %-&!k!& /e,!!% k-7! pe&e&'- ko&/e&'!/% /!mpel"

S!mpel 9!& %-&!k!& %,e%k!& oleh pem,%m,%&" Se'el!h %-k- !& %m!/-kk!&

ke !l!m k-7!( %!p!'l!h ko&/e&'!/% /!mpel 9!%'- (1.C ppm"

I" DATAR PUSTAKA

Se!&( Emel" 2011"( Spek'oo'ome'% U @Ul'!7%ole'"

h''p:66$!&%,e/!k"$ope//"5om" D%!k/e/ p!! '!&!l 12 M!e' 2013"

Hem!&'o( S%&h-" 200"( K!e%&( Se&9!$! Bem!&!!' !'!- Be!5-&k!h"

h''p:66$$$"5hem%/'9"o" D%!k/e/ p!! '!&!l 12 M!e' 2013"

%k%pe%!"( K!e%&!" h''p:66%"$%k%pe%!"o" D%!k/e/ p!! '!&!l 12 M!e' 2013"

S9l7!&!( N%&!" 2012"( Spek'oo'ome'% U" h''p:66/%l7!&!&%&!",lo/po'"5om" D%!k/e/

p!! '!&!l 12 M!e' 2013"

///

Documents

Spektrofotometri UV (DONE)