Special Seminar To invite a researcher from NARO in Japan, we will hold a special seminar on wheat research. Time & Date: 15:30-16:30 on Tue. Jan. 21st, 2020 Venue: Active Learning Space, ALRC Transformation is a key step in modern breeding technology that involves genome editing. The requirement for in vitro tissue culture and regeneration hampers application of this technology to commercially important varieties of many crop species. To overcome this problem, we developed a simple and reproducible in planta transformation method in wheat (Tritticum aestivum L.). Our in planta particle bombardment (iPB) method utilizes the shoot apical meristem (SAM) as a target tissue. The SAM contains a subepidermal cell layer termed L2, from which germ cells later develop during floral organogenesis. The iPB method can also be used for genome editing through transient CRISPR/Cas9 expression or direct delivery of the CRISPR/Cas9 ribonucleoprotein. In this seminar, I will introduce the iPB technology and provide an overview of its current and future applications in plant transformation and genome editing. Dr. Ryozo Imai (Senior Principal Researcher, Institute of Agrobiological Sciences, NARO, Tsukuba, Japan) The in planta Particle Bombardment (iPB) method for cereal transformation and genome editing Contact: Ryosuke Mega & Takayoshi Ishii ([email protected])

Special Seminar - 鳥取大学乾燥地研究センター · Special Seminar Toinvite a researcher from NARO in Japan, we will hold a special seminar on wheat research. Time & Date:

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Special SeminarTo invite a researcher from NARO in Japan, we will hold aspecial seminar on wheat research.

Time & Date: 15:30-16:30 on Tue. Jan. 21st, 2020Venue: Active Learning Space, ALRC

Transformation is a key step in modern breeding technology that involvesgenome editing. The requirement for in vitro tissue culture and regenerationhampers application of this technology to commercially important varietiesof many crop species. To overcome this problem, we developed a simple andreproducible in planta transformation method in wheat (Tritticum aestivumL.). Our in planta particle bombardment (iPB) method utilizes the shootapical meristem (SAM) as a target tissue. The SAM contains a subepidermalcell layer termed L2, from which germ cells later develop during floralorganogenesis. The iPB method can also be used for genome editing throughtransient CRISPR/Cas9 expression or direct delivery of the CRISPR/Cas9ribonucleoprotein. In this seminar, I will introduce the iPB technology andprovide an overview of its current and future applications in planttransformation and genome editing.

Dr. Ryozo Imai (Senior Principal Researcher, Institute of

Agrobiological Sciences, NARO, Tsukuba, Japan)

The in planta Particle Bombardment (iPB) method for cereal transformation and genome editing

Contact: Ryosuke Mega & Takayoshi Ishii ([email protected])