SPE - Phenomenex · SPE Solid Phase Extraction 3 ... strata-X-CW 33 85 800 0.74 strata-X-AW 33 85 800 0.60 Polar and non-Polar Condition: With methanol Equilibrate: With water

Essential for

Improved Sample Preparation:

Strong bases •strata-X-CW, p.12Acids •strata-X-AW, p.13On-line extraction cartridge •Strata C18 20 x 2.0 mm, p.17

SPESolid Phase Extraction

3

StrataSPE

Formats............................................................................................................ 4

Material Characteristics ................................................................................... 5

Phase Selection by Manufacturer.................................................................... 5

EssentialforSPEMethodDevelopment

Selecting the Proper Phase ............................................................................. 6

Selecting the Proper Tube Dimensions ........................................................... 7

Method Development Software ....................................................................... 8

strata-XPolymericSorbents ....................................................................... 9

strata-X .......................................................................................................... 10

strata-X-C ...................................................................................................... 11

strata-X-CW ................................................................................................... 12

strata-X-AW ................................................................................................... 13

StrataOrderingInformation

Tubes ............................................................................................................. 14

StrataGigaTubes .......................................................................................... 15

StrataHigh-Throughput

96-Well Plates ................................................................................................ 16

Protein Precipitation Plates ........................................................................... 16

Accessories ................................................................................................... 17

On-line Format ............................................................................................... 17

Applications ...............................................................................................18-19

SepraBulkSorbents .................................................................................... 20

OtherSPEProducts

3M Empore .................................................................................................... 21

Extraction Disks and Disk Cartridges ...................................................... 21

Extraction Plates ...................................................................................... 21

Protein Precipitation Plates ...................................................................... 21

SPE Vacuum Manifolds ................................................................................. 22

SPE

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STrATASAMPlEPrEPArATIOnPrODucTSAnDSEPrABulkSOrBEnTS• ultra-puresorbentsmanufacturedbyPhenomenex

• consistentlyhighrecoveriesforaccuratequantitationofanalytes

• lot-to-lotreproducibility

• certificateofanalysissuppliedwitheachproduct

• Verytightparticledistributionyieldsoptimizedflowrates

• Outstandingapplicationsupport

Formats

For Traditional Sample Preparation For High-Throughput Screening

For On-line Screening† For Flash Analysis For Flash and Large Scale-Up

†strata-X now available in on-line formatSee p. 17 for ordering information

For Protein Precipitation

Strata is a registered trademark of Phenomenex, Inc. strata-X is a trademark of Phenomenex, Inc.

STRATA® SPE (SOLID PHASE EXTRACTION)If Strata SPE or Sepra bulk products do not perform as well or better than your current SPE product of similar phase, mass and size, send in your comparative data within 45 days and keep the Strata SPE product for FREE!

�Phenomenex

SOLID PHASEEXTRACTION

Strata

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Materialcharacteristics Particle Size Pore Size Surface Area Carbon Load Bonding End Capping Ionic Capacity Phase (µm) (Å) (m2/g) (%) (meq/g)

C18-E 55 70 500 17.0 trifunctional Yes —C18-U 55 70 500 18.0 trifunctional No —C18-T 55 140 300 15.0 trifunctional Yes —C8 55 70 500 10.5 trifunctional Yes —Phenyl 55 70 500 10.5 trifunctional Yes —CN 55 70 500 10.0 trifunctional No —NH2 55 70 500 5.0 trifunctional No 1.3SCX 55 70 500 9.0 trifunctional No 0.9WCX 55 70 500 8.0 trifunctional No 0.8SAX 55 70 500 6.5 trifunctional No 0.9Si-1 (Silica) 55 65 550 0.0 — — —FL-PR (Florisil®) 170 80 300 0.0 — — —

Polymeric

SDB-L 100 260 500 — — — —strata-X 33 85 800 — — — —strata-X-C 33 85 800 — — — 1.0strata-X-CW 33 85 800 — — — 0.74strata-X-AW 33 85 800 — — — 0.60

Mixed Mode

Screen-C GF 200 70 500 proprietary trifunctional — —Screen-C 55 70 500 proprietary trifunctional — —Screen-A 55 70 500 proprietary trifunctional — —ABW 55 70 500 7.0 — — —

**Recommended Alternative - This category indicates an alternative phase which may yield somewhat different selectivity but may also lead to improved resolution.

PhaseSelectionbyManufacturerPhenomenex Waters Varian Supelco JT BAKER IST Macherey-Nagel STRATA ** Sep-Pak Bond Elut Discovery UCT Bakerbond ISOLUTE Chromabond

Reversed Phase

C18-E tC18 C18 DSC-18 C18 Octadecyl C18 (EC) C18ecC18-U PolarPlus C18 C18 Octadecyl Lightload C18 PAHC18-T C18 DSC-18LtC8 C8 C8 DSC-8 C8 Octyl C8 (EC) C8Phenyl (PH) PH DSC-PH Phenyl Phenyl PH (EC)SDB-L ENV DSC-PS/DVB H2O-phobic DVB 101 HR-P LMS PS-RPstrata-X Oasis HLB NEXUS StyreScreen® DVB H2O-philic DVB ENV+ Easy Porapak RDX PPL FOCUS

Normal Phase

Si-1 (Silica) Silica DSC-Silica Silica Silica Gel Silica SiOHFL-PR (Florisil®) Florisil® Florisil® Florisil® Florisil® FL Florisil®

NH2 NH2 NH2 DSC-NH2 Amino Propyl Amino NH2 NH2

CN CN CN-U DSC-CN CN Cyano CN CN Ion Exchange

ABW ENVOLUTE ABN SAX SAX DSC-SAX Quarternary Amine Quarternary Amine SAX SBSCX SCX DSC-SCX Benzene Sulfonic Acid Aromatic Sulfonic Acid SCX SAstrata-X-C Oasis MCX StyreScreen® DBX strata-X-CW Oasis WCX strata-X-AW Oasis WAX WCX CBA DSC-WCX Carboxylic Acid Carboxylic Acid CBA PCAScreen-C Certify I Clean Screen DAU Narc-2 HCX DrugScreen-C GF Certify HF Xtract DAU Screen-A Certify II Clean Screen THC Narc-1 HAX

STRATA® SPE (SOLID PHASE EXTRACTION)

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SPE sorbents are most commonly categorized by the nature of their primary interaction or retention mechanism with the analyte of interest. The three most common extraction mechanisms used in SPE are reversed phase, normal phase and ion exchange.

This concise diagram is designed to assist you in selecting the proper sorbent for your application. For more detailed information, reference books are available (page 372).

SelectingTheProperPhase

Mechanism TypicalApplication

reversedPhase Extraction of hydrophobic or polar organic analytes from aqueous matrix

normalPhase Extraction of polar analytes from non-polar organic solvents

IonExchange Extraction of charged analytes from aqueous or non-polar organic samples

aqueous

water

ionic

organic

organic

neutral

organic

neutral

nonpolar

reversedphase

strata-X

SDB-L

C18

C8

PH

(+)basic

cationexchange

strata-X-C

SCX

WCX

Screen-C

Screen-C GF

(-)acidic

anionexchange

SAX

NH2

Screen-A

polar**

reversedphase*

strata-X

CN

neutral

moderately**polar

reversedphase

strata-X

C8

CN

non**polar

reversedphase

strata-X

SDB-L

C18

C8

PH

moderatelypolar

normalphase

Si-1

NH2

polar

normalphase

CN

Si-1

FL-PR

NH2

(+)basic

cationexchange

strata-X-C

SCX

WCX

Screen-C

Screen-C GF

(-)acidic

anionexchange

SAX

NH2

Screen-A

ionic

strata-X-AW

strata-X-CW strata-X-CW

strata-X-AW

*NOTE: These can be difficult to extract. Consider non-retentive reversed-phase to remove interference.

Polarity logP

Polar log P<1

Mid-Polar(moderately) log P=1-3

non-Polar log P>3

log P is the partition coefficient that can be used to determine the polarity of a compound

**

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STRATA® SPE METHOD DEVELOPMENT

For the latest updates in SPE method development, technical support, and troubleshooting, visit www.strataSPE.com

�Phenomenex


SelectingTheProperTubeDimension

reservoir=Samplecapacity (i.e. 1 mL, 3 mL, 6 mL, 12 mL, 20 mL, 60 mL, 150 mL)

The column volume refers to the dimensions of the SPE tube. It determines the largest volume solvent or sample that can be delivered per aliquot.

SorbentBedMass=Analytecapacity (i.e. 30 mg, 50 mg, 60 mg, 100 mg, 150 mg, 200 mg, 500 mg, 1 g, 2 g, 5 g, 10 g, 20 g, 50 g, 70 g)

The sorbent bed mass determines the maximum amount of analyte that can be retained by the sorbent.

GeneralruletoDetermineProperBedMass

Silica-basedsorbents Non-ionic sorbents retain a mass of solute (analyte plus retained contaminants) that is equivalent to approximately 5 % of the sorbent mass. Therefore, a 100 mg cartridge can retain approximately 5 mg of total solute mass.

Polymericsorbents The large surface area of polymers give a higher capacity that is approximately 10-15 % of the sorbent mass.

Ionexchangesorbents Capacity is determined by the number of charged sites on the sorbent available to interact with the analyte. The mean capacity ranges from 0.8-1.3 meq/ g as shown on page 5.

PoreSize:theaveragediameteroftheporousopeningonthesurfaceofasolventparticle.

For a compound to be extracted in a SPE method, the molecule must not be too large to fit into the pores. For large molecules, sorbents with extra large pores must be used. The average pore size for the Strata sorbents is given on page 5.

Reduce breakthrough by selecting the proper bed mass!

Since the majority of SPE is performed on relatively “dirty” samples that contain many potential contaminants, it is common practice to be conservative and choose a slightly larger sorbent mass.

BedVolume:thesumoftheinterstitialvolumeplustheporevolumeofthesorbent.

The bed volume of the sorbent determines the solvent volumes required in a SPE method. In general, solvent volumes between 4 to 8 times the bed volume are typically necessary to ensure proper conditioning, washing and elution. Conditioning with less than 4 to 8 bed volumes increases the risk of incomplete solvation of the bed and low or irreproducible recoveries, while more than 4 bed volumes is typically unnecessary.

Conventional packed bed, silica-based SPE products typically have a bed volume of approximately 150 µL per 100 mg of sorbent. Polymeric sorbents require larger solvent volumes for proper conditioning, washing and eluting. It is recommended to use 200-250 µL per 100 mg of sorbent.


Strata

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MethodDevelopmentSoftware• InstantlydevelopsanalytespecificSPEmethodson strata-Xsorbents

• Generatesmethodsfortraditionalandhigh-throughput screening

• Providesfast,accurate,andsimpleSPEmethod development


OrDErInGInFOrMATIOn

strata SPE Method Development SoftwarePart No. Description Unit Price

AA0-7814 I strata SPE Method Development I ea

I Software Program on CD-ROM

SuggestedSolidPhaseExtractionMethod

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MethodDevelopmentkits

Threetechniquestochoosefrom...• Samplecleanupandconcentration

• High-throughputscreening

• Proteinprecipitation

OrDErInGInFOrMATIOnSample Preparation Method Development KitsPart No. Description Unit Pricestrata-X Method Development Kit for Sample Cleanup and Concentration

KS0-7908 strata-X 200 mg/3 mL tubes (10 ea) I ea I strata-X-C 200 mg/3 mL tubes (10 ea) strata-X-CW 200 mg/3 mL tubes (10 ea) strata-X-AW 200 mg/3 mL tubes (10 ea) Strata Method Development Demo Softwarestrata-X Method Development 96-Well Plate Kit for High-Throughput Screening

KS0-8241 strata-X 10 mg/well (3 rows) I ea I strata-X-C 10 mg/well (3 rows) strata-X-CW 10 mg/well (3 rows) strata-X-AW 10 mg/well (3 rows)

KS0-8209 strata-X 30 mg/well (3 rows) ea strata-X-C 30 mg/well (3 rows) strata-X-CW 30 mg/well (3 rows) strata-X-AW 30 mg/well (3 rows) Strata Starter Kit for Protein Precipitation

CE0-8201 Strata Impact Protein Precipitation Plate (2 ea) I ea I Collection Plate 2 mL (2 ea) Sealing Mat (2 ea)

Download trial version at www.strataSPE.com

�Phenomenex


Strata-X

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STRATA™-X POLYMERIC SORBENTS

strata-XPOlyMErSurFAcEMODIFIEDSTyrEnEDIVInylBEnzEnE

strata™XSolidPhaseExtraction(SPE)Methods†

Materialcharacteristics Particle Size Pore Size Surface Area Ionic CapacityPhase (µm) (Å) (m2/g) (meq/g)

Polymeric

strata-X 33 85 800 –

strata-X-C 33 85 800 1.0

strata-X-CW 33 85 800 0.74

strata-X-AW 33 85 800 0.60

Polarandnon-PolarCondition: With methanolEquilibrate: With waterLoad Sample: Phosphoric Acid (2 % of total

volume) can be used to disrupt drug and protein interaction

Wash: With 5-60 % methanol in water (organic concentration used in

wash can vary based on nature of analyte) Dry for 1 minute

Elute Analyte: With methanol/acetonitrile (50:50)

strata-X

reversedPhase

Bases Condition: With methanolEquilibrate: With waterLoad Sample: Acidified with 2 % Phosphoric

AcidWash 1: With 0.1 N HCIWash 2: With methanol (elutes acidic

and neutral analytes) This helps in removing endogenous contaminants from biological sample matrices, and eliminates ion suppression in LC/MS under ESI mode.

Elute Analyte: With 5 % ammonium hydroxide/methanol* or acetonitrile

StrongBases Condition: With methanolEquilibrate: With waterLoad Sample: pH of sample should be within

4.5-7.0*Wash 1: With 25 mM ammonium

acetate buffer pH 6.5Wash 2: With methanol (elutes acidic

and neutral analytes) This serves to remove

endogenous contaminants from biological sample matrices, and eliminates ion suppression. Dry for 1 minute

Elute Analyte: With 2 % formic acid in methanol/acetonitrile (20:80)

Depending on analyte solubility and hydrophobicity, isopropanol/methylene chloride (20:80) can be substituted for methanol/acetonitrile

* Samples may need to be acidified to disrupt drug-protein interaction. Also sample should be diluted 1:1 with buffer or water ensuring that sample is two pH units below pKa of analyte and two pH units above the pKa of the sorbent.

StrongAcids Condition: With methanolEquilibrate: With waterLoad Sample: pH of sample should be 2

pH units above the pKa of the acidic analyte to ensure that the analyte is fully deprotonated

Wash 1: With waterWash 2: With methanol (elutes basic

and neutral analytes)Elute: 2 % NH4OH in methanol

N..

n)

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O n)

)O-S

O

O

n)

)O

OH

n)) NH NH2

* For very hydrophobic compounds methanol can be substituted with methylene chloride and/or isopropanol.

* Modified conditioning step(s) such as acidifying the methanol (2 % formic) may be needed to improve selectivity.

†The above is a convenient starting point for SPE method development. Further optimization may be required to tailor the method to your specific needs.

PolymericSurfaceModification• BetterSelectivityforPolarandnon-Polarcompounds

HighAnalytecapacity• BetterretentionforTargetAnalytes

Deconditioningresistant• BetterreproducibilityfromExtractiontoExtraction

strata-X-c

StrongcationExchange&reversedPhase

strata-X-cW

WeakcationExchange&reversedPhase

strata-X-AW

WeakAnionExchange&reversedPhase

10 Phenomenex

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STRATA™-X POLYMERIC SORBENT

rEVErSEDPHASESOrBEnTFOrclEAnuPOFPOlArAnDnOn-POlArcOMPOunDS• Highrecoveriesforacidic,neutralandbasiccompounds

• pHstabilityfrom1-14

• Multipleretentionmechanismsforstreamlinedreversed phasemethoddevelopment

• Extremelycleanextracts

• lc/MScompatibility

• Simplemethodallowsforfastsamplepreparation

EliminatesWell-to-WellVariabilityMost traditional silica-based sorbents will lose their extraction ability if the sorbent dries or becomes ‘deconditioned’. strata-X remains conditioned, yielding extremely high and consistent recoveries, even if the sorbent runs dry!

This is a great feature because it eliminates the possibility of well-to-well variability most commonly associated with silica-based 96-well plates.

conditions:Tubes: 30 mg/1 mL strata-X, 100 mg/1 mL Strata C18-ESamples: Analytes spiked in phosphate buffer saline solution (PBS) at concentration of 1 µg/ mL, n=3Method: The strata-X method was used. After the conditioning step the sorbent was dried under vacuum from 0 up to 10 min at 10" Hg. Percent recoveries were determined by HPLC analysis.

HighAnalytecapacity33 µm strata-X particles have a very high surface area that results in extremely high analyte capacity. strata-X will retain an analyte mass (including potential interference) that is equivalent to 6-15 % of the sorbent bed mass (i.e., a 200 mg cartridge retains approximately 20 mg of total solute mass).

ShorterDryDownTimesThe high analyte capacity of strata-X means that you will need a smaller bed mass which results in lower elution volumes for shorter dry down times and fast sample cleanup.

BetterSelectivityforPolar&Aromaticcompounds

Patent Pending

MaterialcharacteristicsParticle Size (µm): 33Pore Size (Å): 85Pore Volume (mL/g): 1.2Surface Area (m2/g): 800Fine Content (<10 µm by Vol. %): <0.2 %

Excellentfor96-WellPlates

0

% Recovery

10 20 30 40 50 60 70 80 90 100

a: Phenol

b: 2,4,6-tribromophenol

c: 2-�uorophenol

d: 2-chlorophenol

e: 2,4,6-trichlorophenol

f: 2-nitrophenol

g: 2,4-dinitrophenol

h: o-cresol

strata-XSDB

Phenols from Water 10

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Si-based conventional C18

Doxepin

RanitidineCaffeineTheophyllineProcainamide

Drying time (min.)

% R

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OrDErInGInFOrMATIOn

strata-X TubesPart No. Dimensions Unit/Box Price

8B-S100-TAK I 30 mg/1 mL I 100 I

8B-S100-TBJ 30 mg/3 mL 50

8B-S100-UBJ 60 mg/3 mL 50

8B-S100-FBJ 200 mg/3 mL 50

8B-S100-HBJ 500 mg/3 mL 50

8B-S100-ECH 100 mg/6 mL 30

8B-S100-FCH 200 mg/6 mL 30

8B-S100-HCH 500 mg/6 mL 30

strata-X ��-Well PlatesPart No. Dimensions Unit/Box Price

8E-S100-NGB I 5 mg/well I 2 I

8E-S100-AGB 10 mg/well 2

8E-S100-TGB 30 mg/well 2

8E-S100-UGB 60 mg/well 2

strata-X On-Line Extraction CartridgePart No. Dimensions Unit/Box Price

00M-S033-B0-CB I strata-X on-line extraction cartridge, I ea I 25 µm, 20 x 2.0 mm

CH0-5845 Cartridge holder, 20 mm ea

See p. 15 for Giga tube ordering information

11Phenomenex


strata-X-c

TM

STRATA™-X-C POLYMERIC SORBENT

MIXEDMODESTrOnGcATIOnEXcHAnGESOrBEnTFOrclEAnuPOFBASIccOMPOunDS• Enhancedselectivityforaromaticbases

• Deconditioningresistantsurfaceforconsistent sampleprocessing

• 100%organicwashallowsforcompleteremovalof biologicalcontaminants

reducedIonSuppression

FastandFlexibleSamplecleanup

OrDErInGInFOrMATIOn

strata-X-C TubesPart No. Dimensions Unit/Box Price

8B-S029-TAK I 30 mg/1 mL I 100 I

8B-S029-TBJ 30 mg/3 mL 50

8B-S029-UBJ 60 mg/3 mL 50

8B-S029-FBJ 200 mg/3 mL 50

8B-S029-HBJ 500 mg/3 mL 50

8B-S029-ECH 100 mg/6 mL 30

8B-S029-FCH 200 mg/6 mL 30

8B-S029-HCH 500 mg/6 mL 30

strata-X-C ��-Well PlatesPart No. Dimensions Unit/Box Price

8E-S029-AGB I 10 mg/well I 2 I




MaterialcharacteristicsParticle Size (µm): 33Pore Size (Å): 85Surface Area (m2/g): 800pH Stability: 1-14Ionic Capacity: 1 meq/g

Patent Pending

Ion suppression study performed with post column infusion of Atenolol, positive ESI mode m/z 267. Contact Phenomenex for additional method details. Captiva® Protein precipitation plate is a registered trademark of Varian Inc. Phenomenex is in no way affiliated with Varian. May not be representative of all SPE applications shown.

2 µg/mL acrylamide in 0.2 g French friesStreamlined method development

2.92

2 -

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Ansys Captiva® Protein Precipitation Plate

strata-X-C

Intens.

x10

2.0

1.5

1.0

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0 1 2 3 4 5 6 min

SingleStepcleanupofAcrylamidefromFriedPotatoes

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>78 % ≥84 % >91 %

Acrylamide

500

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2000

ng/

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TN-0

07

Sorbent: strata-X-C 200 mg/3 mL Part No.: 8B-S029-FBJCondition: 2 x 1 mL methanolEquilibrate: 2 x 1 mL water Load: 1 mL homogenized supernatant, dry for 30 secElute: 1 mL water at a flow rate of 0.5 mL/ minEvaporate: remove excess water

HPLC Column: Synergi™ 4 μm Polar-RP™ 150 x 3.0 mmPart No.: 00F-4336-Y0Sample: 10 µL of extractMobile Phase: water / acetonitrile (94:6)Flow Rate: 0.4 mL/ minTemperature: 25 °CDetector: UV @ 210 nm

12 Phenomenex

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1 2 3 4 5 6 7 0

C: Spiked Plasma Extract

B: Blank Plasma Extract

A: Standard Solution

1 2 3 4 5 6 7 0

EIC 304 All

TIC All Standard: Benzalkonium Chloride

A: Standard Solution

Plasma Extracts (LC/MS extracted ions m/z; 304(C12) and 332(C14))

+ -

+ -

EIC 332 All + -

1 2 3 4 5 6 7 0

EIC 304 All

TIC All Elute Fraction

+ -

+-

EIC 332 All + -

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ExtractionofBenzalkoniumchloridefromPlasmaSorbent: strata-X-CW 30 mg/1 mLPart No.: 8B-S035-TAKCondition: 1 mL methanol Equilibrate: 1 mL waterLoad: Analyte spiked human plasma (350 µL plasma + 700 µL water and acidify with 20 µL phosphoric acid)Wash 1: 1 mL 25 mM sodium acetate buffer pH 4.5, Dry for 1 minWash 2: 1 mL methanolElute: 1 mL 5 % formic acid/methanol Inject the elution fraction onto the LC-MS (no need to dry down)

HPLC Column: Luna® 5 µm CN 150 x 2.0 mmPart No.: 00F-4255-B0Mobile Phase: A: 75 mM ammonium acetate (pH 5.0), B: acetonitrileIsocratic: A/B (40:60)Flow Rate: 0.2 mL/ minMS Instrument: Bruker Esquire 2000 ITSource: ESI-Positive modeSamples: C12 Benzalkonium Chloride (100 ng/ mL) C14 Benzalkonium Chloride (100 ng/ mL)

0

20

40

60

80

100

% R

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Condition: Condition:

C12

Benz

alko

nium

Chl

orid

e

C14

Benz

alko

nium

Chl

orid

e

No Dry Down Needed

MIXEDMODEWEAkcATIOnEXcHAnGESOrBEnTFOrclEAnuPOFSTrOnGBASES• Streamlinedmethoddevelopmentforfasteranalysis

• Weakcationexchangemechanismallowsforcapture andreleaseofquaternaryamines

• π-π,hydrophobic,andpolarretentionoftargetanalytes

ExtractionofclidiniumBromidefromPlasmaSorbent: strata-X-CW 30 mg/1 mLPart No.: 8B-S035-TAKCondition: 1 mL methanol Equilibrate: 1 mL waterLoad: Analyte spiked human plasma (350 µL plasma + 700 µL water and acidify with 20 µL phosphoric acid)Wash 1: 1 mL 25 mM sodium acetate buffer pH 4.5, Dry for 1 minWash 2: 1 mL methanolElute: 1 mL 5 % formic acid/methanol Inject the elution fraction onto the LC/MS (no need to dry down)

HPLC Column: Luna® 5 µm CN 150 x 2.0 mmPart No.: 00F-4255-B0Mobile Phase: A: 75 mM ammonium acetate (pH 5.0), B: acetonitrileIsocratic: A/B (35:65)Flow Rate: 0.2 mL/ minMS Instrument: Bruker Esquire 2000 ITSource: ESI-Positive modeSample: Clidinium Bromide (50 ng/ mL)

stra

ta-X

-cW

STRATA™-X-CW POLYMERIC SORBENT

No Dry Down Needed

TM

SelectiveElutionofQuaternaryAmines

EliminateDryDownandreconstitutionSteps!

CN-0

27CN

-025

OrDErInGInFOrMATIOn

strata-X-CW TubesPart No. Dimensions Unit/Box Price

8B-S035-TAK I 30 mg/1 mL I 100 I

8B-S035-UBJ 60 mg/3 mL 50

8B-S035-FBJ 200 mg/3 mL 50

8B-S035-HBJ 500 mg/3 mL 50

8B-S035-ECH 100 mg/6 mL 30

8B-S035-FCH 200 mg/6 mL 30

8B-S035-HCH 500 mg/6 mL 30

strata-X-CW ��-Well PlatesPart No. Dimensions Unit/Box Price




strata-X-CW On-Line Extraction CartridgePart No. Dimensions Unit/Box Price

00M-S036-B0-CB I strata-X-CW on-line extraction cartridge, I ea I 20 x 2.0 mm



13Phenomenex


strata-X-A

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STRATA™-X-AW POLYMERIC SORBENT

No Dry Down Needed

No Dry Down Needed

TM

OrDErInGInFOrMATIOn

strata-X-AW TubesPart No. Dimensions Unit/Box Price

8B-S038-TAK I 30 mg/1 mL I 100 I

8B-S038-UBJ 60 mg/3 mL 50

8B-S038-FBJ 200 mg/3 mL 50

8B-S038-HBJ 500 mg/3 mL 50

8B-S038-ECH 100 mg/6 mL 30

8B-S038-FCH 200 mg/6 mL 30

8B-S038-HCH 500 mg/6 mL 30

strata-X-AW ��-Well PlatesPart No. Dimensions Unit/Box Price




EfficientExtractionofnucleotides

SelectiveElutionofAcidicAnalytes

MIXEDMODEWEAkAnIOnEXcHAnGESOrBEnTFOrclEAnuPOFAcIDS• Streamlinedmethoddevelopmentforfasteranalysis

• Selectiveelutionofacidiccompounds

• π-π,hydrophobic,andpolarretentionoftargetanalytes

0 0.5 1 1.5 2 2.5 3 3.5 4 min

0

100

200

300

mAU

400

min0 1 2 3 4 5 6 7 8

mAU

0

50

100

150

200

2501

2

4

Adenosine Sorbent: strata-X-AW 30 mg/1 mLPart No.: 8B-S038-TAKCondition 1: 1 mL methanol Condition 2: 1 mL formic acid / methanol / water (2:25:73)Equilibrate: 1 mL waterLoad: 1 mL 0.2 mg/mL in phosphate buffered saline / water (1:1) Wash 1: 1 mL waterWash 2: 1 mL methanol / water (50:50)Elute: 1 mL NH4OH / methanol / water (2:25:78) Elution fraction 1:1 with 25 % methanol / 20 mM NH4 acetate, pH 5.3 prior to injection (no need to dry down or reconstitution)

HPLC Column: Gemini® 5 µm C18 110 Å 150 x 4.6 mmPart No.: 00F-4435-E0Mobile Phase: 25 % acetonitrile / 10 mM KH2HPO4, pH 7.0 / 5 mM TBACInjection: 5 µLFlow Rate: 1.0 mL/minTemperature: 30 ˚CDetection: UV @ 260 nmRecovery Data: Sample % Recovery % RSD 1. 5' AMP 81 2.0 2. 5' ADP 90 0.3 3. 5' ATP 92 0.1

ExtractionofOrtho-Phosphotyrosine Sorbent: strata-X-AW 30 mg/1 mLPart No.: 8B-S038-TAKCondition 1: 1 mL methanol Condition 2: 1 mL formic acid / methanol / water (2:25:73)Equilibrate: 1 mL waterLoad: 1 mL 200 µg/mL in phosphate buffered saline / water (1:3)Wash 1: 1 mL waterWash 2: 1 mL methanol / water (50:50)Elute: 1 mL NH4OH / methanol / water (5:50:45) Elution fraction 1:1 with water prior to injection (no need to dry down or reconstitute)

HPLC Column: Gemini® 5 µm C18 110 Å 150 x 4.6 mmPart No.: 00F-4435-E0Mobile Phase: 20 % acetonitrile / 10 mM KH2HPO4, pH 7.0 / 5 mM TBACInjection: 10 µLFlow Rate: 1.0 mL/minTemperature: 30 ˚CDetection: UV @ 220 nmRecovery Data: Sample % Recovery % RSD Ortho-Phosphotyrosine 99 3

CN-0

40CN

-039


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STRATA® SPE ORDERING INFORMATION

Silica-based sorbentsPhase 50 mg 100 mg 100 mg 200 mg 500 mg — 500 mg 1 g

I I I I I

C18-E 8B-S001-DAK 8B-S001-EAK 8B-S001-EBJ 8B-S001-FBJ 8B-S001-HBJ — 8B-S001-HCH 8B-S001-JCHC18-U — 8B-S002-EAK — 8B-S002-FBJ — — 8B-S002-HCH —C18-T — 8B-S004-EAK — 8B-S004-FBJ 8B-S004-HBJ — 8B-S004-HCH 8B-S004-JCHC8 — 8B-S005-EAK — 8B-S005-FBJ 8B-S005-HBJ — 8B-S005-HCH 8B-S005-JCHPhenyl — 8B-S006-EAK — 8B-S006-FBJ 8B-S006-HBJ — — 8B-S006-JCH

SCX — 8B-S010-EAK 8B-S010-EBJ 8B-S010-FBJ 8B-S010-HBJ — 8B-S010-HCH 8B-S010-JCHWCX — 8B-S027-EAK — 8B-S027-FBJ 8B-S027-HBJ — 8B-S027-HCH 8B-S027-JCHSAX — 8B-S008-EAK 8B-S008-EBJ 8B-S008-FBJ 8B-S008-HBJ — 8B-S008-HCH 8B-S008-JCHNH2 — 8B-S009-EAK — 8B-S009-FBJ 8B-S009-HBJ — 8B-S009-HCH 8B-S009-JCHCN — 8B-S007-EAK — 8B-S007-FBJ 8B-S007-HBJ — 8B-S007-HCH 8B-S007-JCH

Si-1 — 8B-S012-EAK — 8B-S012-FBJ 8B-S012-HBJ — 8B-S012-HCH 8B-S012-JCHFlorisil — — — — 8B-S013-HBJ — 8B-S013-HCH 8B-S013-JCHEPH — — — — 8B-S031-HBJ — — —

Mixed-mode sorbents (for drugs of abuse)Phase — — — 200 mg 500 mg 200 mg 500 mg 1 g

I I I I I

Screen-C GF — — — 8B-S026-FBJ 8B-S026-HBJ 8B-S026-FCH 8B-S026-HCH 8B-S026-JCH

Phase — 100 mg 100 mg 150 mg 200 mg 300 mg 150 mg 500 mg —

I I I I I I

Screen-C — 8B-S016-EAK 8B-S016-EBJ 8B-S016-SBJ 8B-S016-FBJ 8B-S016-RBJ 8B-S016-SCH 8B-S016-HCH —Screen-A — 8B-S019-EAK — — 8B-S019-FBJ — — 8B-S019-HCH —

Polymeric sorbentsPhase — 100 mg — 200 mg 500 mg — 500 mg 1 g

I I I I I

SDB-L — 8B-S014-EAK — 8B-S014-FBJ 8B-S014-HBJ — 8B-S014-HCH 8B-S014-JCH

Phase 30 mg — 60 mg 200 mg 500 mg 100 mg 200 mg 500 mg

I I I I I

strata-X 8B-S100-TAK — 8B-S100-UBJ 8B-S100-FBJ 8B-S100-HBJ 8B-S100-ECH 8B-S100-FCH 8B-S100-HCH

strata-X-C 8B-S029-TAK — 8B-S029-UBJ 8B-S029-FBJ 8B-S029-HBJ 8B-S029-ECH 8B-S029-FCH 8B-S029-HCHstrata-X-CW 8B-S035-TAK — 8B-S035-UBJ 8B-S035-FBJ 8B-S035-HBJ 8B-S035-ECH 8B-S035-FCH 8B-S035-HCHstrata-X-AW 8B-S038-TAK — 8B-S038-UBJ 8B-S038-FBJ 8B-S038-HBJ 8B-S038-ECH 8B-S038-FCH 8B-S038-HCH

Strata Tubes

See p. 16 for 96-well plate

ordering information

1ml(100/box) 3ml(50/box) 6ml(30/box)

30 mg 50 mg 100 mg 60 mg 100 mg 150 mg 200 mg 300 mg 500 mg 100 mg 150 mg 200 mg 500 mg 1 g

OrDErInGInFOrMATIOn Note: Tube sizes not proportional nor actual size.

If Strata SPE products do not perform as well or better than your current SPE product of similar phase, mass and size, send in your comparative data within 45 days and keep the Strata SPE product for FREE!

1�Phenomenex


Strata

Gig

aTubes

STRATA® GIGA™ TUBES

�00 mg 1 g 1 g 12 mL 12 mL 20 mL (20/box) (20/box) (20/box)

75 mg 150 mg 150 mg

10 mL 20 mL 20 mL

�00 mg 2 g � g 10 g 20 g 12 mL 12 mL 20 mL 60 mL 60 mL (20/box) (20/box) (20/box) (16/box) (16/box)

Retention Capacity 25 mg 100 mg 250 mg 500 mg 1000 mg

Suggested Elution Volume 6 mL 24 mL 60 mL 120 mL 240 mL

• Pre-packedformatseliminateexcesslaborassociatedwithglasspackedflashcolumns

• Highcapacityformatsavailablein12,20,60,and150mltubesizes

• narrowparticledistributionprovidesconsistentandevenflow

• Greatforpurificationoforganiccompounds

Recommended Capacity and Elution Volumes for Strata Giga Tubes†

Silica-Based Sorbents C18, C8, Phenyl, SCX, SAX, NH2, CN, Silica(Si-1), FL-PR (Florisil®), ABW, EPH

Strata Giga Tubes 12 mL (20/box) 20 mL (20/box) 60 mL (16/box) 150 mL (8/box)Phase 500 mg 1 g 2 g 1 g 5 g 10 g 20 g 50 g 70 g

C18-E 8B-S001-HDG — 8B-S001-KDG — 8B-S001-LEG 8B-S001-MFF 8B-S001-VFF 8B-S001-YSN 8B-S001-ZSNC8 — — 8B-S005-KDG — 8B-S005-LEG 8B-S005-MFF — — —Phenyl — — 8B-S006-KDG — — — — — —

SCX — — 8B-S010-KDG — 8B-S010-LEG 8B-S010-MFF 8B-S010-VFF — —SAX 8B-S008-HDG — 8B-S008-KDG — 8B-S008-LEG 8B-S008-MFF 8B-S008-VFF — —NH2 8B-S009-HDG — 8B-S009-KDG — 8B-S009-LEG 8B-S009-MFF 8B-S009-VFF — —CN — — 8B-S007-KDG — 8B-S007-LEG — — — —

Si-1** 8B-S012-HDG — 8B-S012-KDG — 8B-S012-LEG 8B-S012-MFF 8B-S012-VFF 8B-S012-YSN 8B-S012-ZSNFL-PR (Florisil®) — — 8B-S013-KDG — 8B-S013-LEG 8B-S013-MFF — — —

strata-X 8B-S100-HDG 8B-S100-JDG — 8B-S100-JEG — — — — —

strata-X-C 8B-S029-HDG 8B-S029-JDG — — — — — — —

strata-X-CW 8B-S035-HDG 8B-S035-JDG — 8B-S035-JEG — — — — —

strata-X-AW 8B-S038-HDG 8B-S038-JDG — — — — — — —

strata ABW — — 8B-S030-KDG — 8B-S030-LEG 8B-S030-MFF — — —

strata EPH** — — — — 8B-S031-LEG — — — —

OrDErInGInFOrMATIOn

See p. 22 for Giga Tube & Vacuum Manifold Accessories

Polymeric Sorbentsstrata-X, strata-X-C, strata-X-CW, strata-X-AW

† Retention capacities and elution volumes are specific to the chemical nature of the analyte being extracted, its concentration in the sample, the chemical nature of the eluting solvent and the bed mass used. The above are only guidelines. An elution study should be conducted. As a general rule, recommended solvent wash and elution volumes for silica-based material should be 1.2 mL per 100 mg of sorbent and 2 mL per 100 mg of polymeric sorbent,

** Teflon-coated tubes available, contact Phenomenex for details.

Note: Tube sizes not proportional nor actual size.


1� Phenomenex

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Contact Phenomenex or your local Phenomenex distributor for latest 96-well plate technical note.

STRATA® HIGH-THROUGHPUT

��-Well PlatesPhase 5 mg 10 mg 25 mg 30 mg 50 mg 60 mg 100 mg

I I I I I I

strata-X-C — 8E-S029-AGB — 8E-S029-TGB — 8E-S029-UGB —strata-X-CW — 8E-S035-AGB — 8E-S035-TGB — 8E-S035-UGB —strata-X-AW — 8E-S038-AGB — 8E-S038-TGB — 8E-S038-UGB —

strata-X 8E-S100-NGB 8E-S100-AGB — 8E-S100-TGB — 8E-S100-UGB —SDB-L — — — — 8E-S014-DGB — — Screen-C — — — — 8E-S016-DGB — —Screen-A — — 8E-S019-CGB — 8E-S019-DGB — — C18-E — — 8E-S001-CGB — 8E-S001-DGB — 8E-S001-EGBC18-U — — — — 8E-S002-DGB — —C8 — — 8E-S005-CGB — 8E-S005-DGB — 8E-S005-EGBPhenyl — — — — 8E-S006-DGB — 8E-S006-EGBNH2 — — 8E-S009-CGB — 8E-S009-DGB — —CN — — 8E-S007-CGB — 8E-S007-DGB — —WCX — — 8E-S027-CGB — 8E-S027-DGB — 8E-S027-EGBSCX — — 8E-S010-CGB — — — 8E-S010-EGBSAX — — 8E-S008-CGB — 8E-S008-DGB — 8E-S008-EGBSi-1 — — — — 8E-S012-DGB — —

96-WellPlates• upto2mlsamplevolumeperwell

• compatiblewithmostautomatedsystemsincluding:

• Zymark SciCLONE • TomTec Quadra-96 • Beckman Coulter Biomek 2000 • Gilson 215

StrataMethodDevelopment96-WellPlateforrapidScreening• EachStrata96-wellplatecontainssixsilica-based sorbentsandtwopolymericresins.Onerowofc18-E, c18-u,c18-M,c18-T,c8,PH(50mg/well)andstrata-X andSDB-l(30mg/well)forparallelscreening

OrDErInGInFOrMATIOn(2PlATES/BOX)

Strata Method Development ��-Well PlatePart No. Unit/Box Price

KS0-7293 I 1 ea I

OrDErInGInFOrMATIOn

StrataImpactProteinPrecipitationPlates• SolventShieldingTechnology™forclean,leak-freeresults

• Versatileformatsforfastsampleprocessing

Strata Impact Precipitation PlatesPart No. Description Unit/Box Price

CE0-7565 I Strata Impact Square Well Plate, 2 mL I 2 I

OrDErInGInFOrMATIOn(2PlATES/BOX)

2 Plates/Box

See p. 8 for Strata-X Method Development Kits

See p. 8 for Strata Starter Kit for Protein Precipitation


1�Phenomenex


Strata

Hig

h-Thro

ughp

utAccesso

ries&O

n-linecartrid

ges

HandbookofBioanalysisandDrugMetabolismEdited by Gary Evans

Partno.:AA0-8224Price:

Solid-PhaseExtraction:PrinciplesandPracticeBy E.M. Thurman. 1998, 352pp

Partno.:AA0-4567Price:

Contact Phenomenex or your local Phenomenex distributor for Free SPE technical notes.

STRATA® HIGH-THROUGHPUT ACCESSORIES

Manifold• Madeofdurableacrylic

• FitsStratacollectionplates(0.35,1and2ml)

• Affordable

Collection Plates*Part No. Description Unit Price

AH0-7192 I 0.35 mL/well 96 square well collection plate I 50/pk I

AH0-7193 1 mL/well 96 square well collection plate 50/pk

AH0-7194 2 mL/well 96 square well collection plate 50/pk

AH0-7279 1 mL/well 96 round well collection plate 50/pk

AHO-7408 Solvent waste reservoir tray 25/pk

Replacement PartsPart No. Description Unit Price

AH0-7198 I 96-Well Plate Manifold replacement gasket, profile I 1 ea I (to fit between top plate and vacuum chamber)

AH0-7285 96-Well Plate Manifold replacement gasket, flat 1 ea (to fit between lid and plate)

Manifold*Part No. Description Unit Price

AH0-7284 I 96-Well Plate Manifold, Acrylic I 1 ea I

OrDErInGInFOrMATIOn

96-WellPlate&ProteinPrecipitationPlateAccessories

SealingMatsandcollectionPlates• Preservessampleintegrity

• Maximizessamplerecovery

• noadhesiveswhichcancauseinterference

• Madeofchemicallyresistantpolypropylene

*Manifold, Collection plates, & Sealing mats compatible with 2 mL Impact plate and strata 96-well plate formats only.

strata On-Line Extraction CartridgePart No. Dimensions Unit/Box Price

00M-S033-B0-CB I strata-X on-line extraction cartridge, I ea I 20 x 2.0 mm

00M-S036-B0-CB strata-X-CW on-line extraction cartridge, ea 20 x 2.0 mm

00M-S039-B0-CB Strata C18 on-line extraction cartridge, ea 20 x 2.0 mm

00M-S101-B0-CB Strata C8 on-line extraction cartridge, ea 20 x 2.0 mm


OrDErInGInFOrMATIOn

Sealing Mats*Part No. Description Unit Price

AH0-7195 I Pierceable sealing mats, 96 square well I 50/pk I

AH0-7362 Sealing tape pad 10/pk

OrDErInGInFOrMATIOn

FlOW

On-lineFormat

Strataon-lineextractioncartridges• Extremelyrapidmethoddevelopment

• Eliminationofstorage

• reducedsamplepreparationtime

• longcolumnlifetimes

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STRATA™-X APPLICATIONS

0

10

20

30

40

50

60

70

80

90

100

% R

ecov

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101% 103% 100%

ExtractionofPolarSteroidsfromSerum

1 3

2

4

BA

CN-0

08

100 % organic wash results in clean extracts

min 20 4 6 8 10 12

21

B

A0

10

20

30

40

50

60

70

80

90

100

% R

ecov

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95%

Methadonefromurine

min0 2 4 6 8 10 11 12

1

2

3 4

High recoveries by strong cationexchange retention mechanism

B

A

Barbituratesfromurine

CN-0

14CN

-005

Met

hado

ne

Phen

obar

bita

l

Buta

lbita

l

Amob

arbi

tal

ExtremelycleanExtracts

HighrecoveriesforDrugsofAbuse

Reversed Phase

Mixed Mode Cation Exchange

Mixed Mode Cation Exchange

Sorbent: strata-X 30 mg/1 mL Part No.: 8B-S100-TAK Condition: 1 mL methanol Equilibrate: 1 mL water Load: 1 mL porcine spiked with analyte Wash: 1 mL 5 % methanol in water Elute: 1 mL methanol Evaporate: add 25 µL of 0.1 mg/mL butyl paraben (external standard) dry down under slow stream of N2 and reconstitute in 200 µL acetonitrile

HPLC Column: Synergi™ Max-RP 4 µm 150 x 4.6 mm SecurityGuard™ C18 4 x 3.0 mm Part No.: 00F-4337-E0 and AJ0-4287 Sample: 50 µL of reconstituted extract Mobile Phase: A: 20 mM KH2PO4 (pH 7.0) B: methanol A/B (35:65) for 20 min Flow Rate: 1.0 mL/min Temperature: 25 °C

Detector: UV @ 254 nm Peaks: 1. Prednisolone 2. Betamethasone 3. Butyl Paraben (external std) 4. Betamethasone Valerate Chromatogram of Extracts: A) Blank B) Spiked Sample

Sorbent: strata-X-C 60 mg/3 mL Part No.: 8B-S029-UBJCondition: 2 mL methanolEquilibrate: 2 mL waterLoad: 3 mL urine spiked with analytes and IS (0.5 µg/mL); dilute 1:1 with 100 mM KH2PO4, pH 6; acidified with 2 % phosphoric acidWash 1: 2 mL 0.1 N HCIWash 2: 2 mL methanolElute: 2 mL 5 % NH4OH/methanolEvaporate: dry down and reconstitute with 200 µL mobile phase

HPLC Column: Synergi™ Max-RP 4 μm 150 x 4.6 mmPart No.: 00F-4337-E0Sample: 50 µL of reconstituted urine extractMobile Phase: A: 0.1 % TFA in water, pH 2.1 B: acetonitrite A/B (65:35)Flow Rate: 1.0 mL/minTemperature: 25 °C

Detector: UV @ 210 nmPeaks: 1. Estazolam (IS) 2. MethadoneChromatogram of Extracts: A) Blank B) Spiked Sample

Sorbent: strata-X-C 60 mg/3 mL Part No.: 8B-S029-UBJCondition: 2 mL methanolEquilibrate: 2 mL waterLoad: 2 mL human urine spiked with analytes and IS (0.5 µg/mL); dilute 1:1 with water Wash 1: 2 mL 5 % methanol in waterWash 2: 2 mL 10 % methanol in 2 % acetic acid and dry for 1 minElute: 2 mL 35 % methanol/2 % NH4OHEvaporate: dry down under slow stream of N2 and reconstitute in 300 µL mobile phase

HPLC Column: Synergi™ Max-RP 4 μm 150 x 4.6 mmPart No.: 00F-4337-E0Sample: 50 µL of reconstituted urine extractMobile Phase: A: 20 mM KH2PO4, pH 7.0 B: acetonitrile Gradient: A/B: (80:20) hold 3 min to (50:50) in 12 min Flow Rate: 1.0 mL/minTemperature: 25 °C

Detector: UV @ 214 nmPeaks: 1. Phenobarbital 3. Amobarbital 2. Butalbital 4. Secobarbital (IS)Chromatogram of Extracts: A) Blank B) Spiked Sample

1�Phenomenex


STRATA® EPH APPLICATIONS

StrataA

pp

lications

c9-c18andc19-c36 c11-c22

AliphaticHydrocarbons AromaticHydrocarbons

0 5 10 15 20 25 30 35

1

2

5

6 3 4

7 8 9

10

11

12 13 14 15 16

17

min

Compound % Recovery % RSD (N=3)

n-Nonane (C9) 88.4 2.3 n-Decane (C10) 91.9 2.3 n-Dodecane (C12) 92.8 2.2 n-Tetradecane (C14) 93.2 2.2 n-Hexadecane (C16) 94.2 2.3 n-Octadecane (C18) 93.5 2.2 n-Nonadecane (C19) 91.1 1.9 n-Eicosane (C20) 92.8 1.9 n-Docosane (C22) 92.9 1.8 n-Tetracosane (C24) 92.2 1.6 n-Hexacosane (C26) 92.4 1.6 n-Octacosane (C28) 93.4 1.5 n-Triacotane (C30) 95.9 1.4 n-Hexatriacontane (C36) 111.6 0.8 Surrogate 46.1 —

Compound % Recovery % RSD (N=3)

Naphthalene 67.2 2.22-Methylnaphthalene 72.1 1.7Acenaphthalene 72.9 1.0Acenaphthene 76.2 1.3Flourene 97.9 2.2Phenanthrene 92.8 2.7Anthracene 84.0 2.2Fluoranthene 84.3 2.2Pyrene 84.1 2.2Benz[a]anthracene 88.3 2.0Chrysene 87.4 2.0Benzo[b]fluoranthene 97.0 2.4Benzo[k]fluoranthene 90.6 1.7Benzo[a]pyrene 91.9 1.7Indeno[1,2,3-cd]pryrene 91.4 1.1Dibenz[a,h]anthracene 94.8 1.2Benzo[g,h,i]perylene 92.7 1.1Surrogate 90.5 —

1 2

3

4

5

6

7

8 9 10

11 12

13

14

15 16 17

18 19

20

21 22

23

0 5 10 15 20 25 30 35 min

GC Analysis ConditionsGC Column: Zebron™ ZB-5ms Part No.: 7HM-G010-11Dimensions: 30 meter x 0.32 mm x 0.25 µmInjection: 2 µL splitless @ 285 °CCarrier Gas: Helium @ 3 mL/min Oven Program: 60 °C for 1 minute then ramp to 290 °C at 8 °C/min hold for 6.75 minutes Detector: FID @ 315 °C

ExtractablePetroleumHydrocarbons(EPH)FractionationMethod

HighrecoveriesforExtractablePetroleumHydrocarbons(50ppm)

Sample Preparation: Solid Phase Extraction ConditionsSorbent: Strata® EPH 5 g/20 mLPart No.: 8B-S031-LEGEquilibrate: 30 mL HexaneLoad: SampleElute Aliphatics C9-C18 and C19-C36: 11 mL Hexane (Lower elution volume allows for more concentrated fraction and faster sample dry down times)Elute Aromatics C11-C22: 20 mL Methylene ChlorideConcentrate: Samples to 1 mL

Peak Compound Ret. Time % RSD 1 C9 3.018 4.37 2 C10 4.283 4.87 3 C12 7.458 5.30 4 C14 10.662 5.67 5 Butylhydroxytoluene 12.315 6 C16 13.592 5.66 7 C18 16.240 5.31 8 C19 17.473 5.11 9 C20 18.648 5.10 10 5-a-Androstane (IS) 18.903 11 1-Chloro-Octadecane (surr) 19.671 12 C22 20.848 4.75 13 C24 22.871 4.24 14 C26 24.741 3.47 15 C28 26.478 2.65 16 C30 28.098 2.29 17 C36 33.536 8.02

Peak Compound Ret. Time % RSD 1 Naphthalene 7.392 11.67 2 2-Methylnaphthalene 9.185 12.59 3 2-Fluorobiphenyl (Frac Surr) 10.307 4 Acenaphthalene 11.602 13.12 5 2-Bromonaphthalene (Frac Sur) 11.985 6 Acenaphthene 12.086 13.24 7 Phthalate 12.313 8 Fluorene 13.544 13.14 9 Phenanthrene 16.215 13.04 10 Anthracene 16.354 12.82 11 o-Terphenyl (Surr) 17.488 12 5-a-Androstane 18.900 13 Fluoranthene 19.568 13.23 14 1-Chloro-Octadecane 19.667 (surr-aliphatic) 10.620 15 Pyrene 20.169 10.24 16 Benz[a]anthracene 23.627 12.22 17 Chrysene 23.724 12.30 18 Benzo[b]fluoranthene 26.477 11.59 19 Benzo[k]fluoranthene 26.546 11.84 20 Benzo[a]pyrene 27.245 11.34 21 Indeno[1,2,3-cd]pryene 29.732 10.62 22 Dibenz[a,h]anthracene 29.829 10.96 23 Benzo[g,h,i]perylene 30.274 10.58

20 Phenomenex

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SEPRA™ BULK SORBENTS

Sep

raB

ulk

So

rben

ts

Interested in MSPD for your analysis? Please contact us for technique and accessory information.

Bondesil® is a registered trademark of Varian Inc. ISOLUTE® is a registered trademark of International Sorbent Technology Ltd. Florisil® is a registered trademark of U.S. Silica

Sepra Bulk SorbentsPhase 100g Price 1kg Price

Reversed Phase I I I I

C18-E 04G-4348 04K-4348 C18-T 04G-4405 04K-4405 C8 04G-4406 04K-4406 Phenyl 04G-4407 04K-4407 CN 04G-4409 04K-4409 SDB-L 04G-4412 04K-4412

Normal Phase

Silica 04G-4410 04K-4410 FL-PR 04G-4411 04K-4411 (Florisil® Pesticide Residue)

Ion Exchange

SCX 04G-4413 04K-4413 SAX 04G-4414 04K-4414 NH2 04G-4408 04K-4408

Materialcharacteristics Particle Size Pore Size Surface Area Carbon Load Bonding End Capping pH Stability Ionic CapacityPhase (µm) (Å) (m2/g) (%) (meq/g)

Reversed Phase

C18-E 50 65 520 17.0 trifunctional Yes 1 to 8 –

C18-T 50 140 300 15.0 trifunctional Yes 1 to 8 –

C8 50 65 520 10.5 trifunctional Yes 1 to 8 –

Phenyl 50 65 520 10.5 trifunctional Yes 1 to 8 –

CN 50 65 520 9.8 trifunctional No 1 to 8 –

SDB-L 95 225 520 – – No 1 to 14 –

Normal Phase

Silica 50 65 300 – – No 1 to 8 –

FL-PR (Florisil® Pesticide Residue) 165 75 300 – – No 1 to 8 –

Ion ExchangeSCX 50 65 520 9.0 trifunctional No 1 to 8 0.9

SAX 50 65 520 6.5 trifunctional No 1 to 8 0.9

NH2 50 65 520 5.0 trifunctional No 1 to 8 1.3

• Availableinpolarandnon-polarphasesfor widerangeofselectivities

• AgoodalternativetoVarianBondesil®and ISOluTE®bulksorbents

• Availableingramtomulti-kilogramquantities

• Economical

• rugged

Sepra high performance bulk SPE sorbents are excellent for applications such as MSPD (matrix solid phase dispersion) in which drugs of interest are separated from homogenized animal tissue or food sources. These high quality sorbents can also be used for flash analysis to effectively purify organic compounds.

OrDErInGInFOrMATIOn

If Sepra Bulk products do not perform as well or better than your current SPE product of similar phase, mass and size, send in your comparative data within 45 days and keep the Sepra Bulk for FREE!

21Phenomenex


3M EMPORE™

3ME

mp

ore

For fast and easy sample extraction, Phenomenex offers filtration glassware for Empore Extraction Disks. Our 47 mm all-glass microfiltration units are used for individual extractions and are described on page 350. Three- and six-station Extraction Manifolds are also available, please inquire.

Empore Extraction DisksPart No. Mfr. No. Description Disk Diameter (mm) Unit PriceAH0-2540 I 2215 I C18 I 47 I 20/pk I

AH0-2541 2315 C18 90 10/pk

AH0-2543 2214 C8 47 20/pk

AH0-3482 2252 Anion-Exchange 47 20/pk

AH0-3485 2240 SDB-XC 47 20/pk

AH0-4048 2241 SDB-RPS 47 20/pk

AH0-3488 2270 Oil and Grease 47 20/pk

Empore Disk CartridgesPart No. Mfr. No. Description Size* Unit PriceAH0-4056 I 4115 I C18-SD** I 4 mm/1 mL I 100/pk I

AH0-4057 4215 C18-SD*a* 7 mm/3 mL 50/pk

AH0-4058 4315 C18-SD** 10 mm/6 mL 30/pk

* Disk diameter/cartridge volume. ** SD= Standard Density is optimized for biological matrices (40 µm particle, enhanced flow characteristics).

Filtration GlasswarePart No. Description Disk Diameter (mm) Unit Price

Filtration Glassware Complete Assemblies I I I AH0-1566 FilterSys, Filtration Glassware, All-Glass, 300 mL funnel with 1 L flask 47 ea

AH0-3314 FilterSys, Filtration Glassware, All-Glass, 500 mL funnel with 2 L flask 47 ea

AH0-3315 FilterSys, Filtration Glassware, All-Glass,1000 mL funnel with 4 L flask 47 ea

Filtration Glassware Component Parts AH0-1567 Fritted Support Base, 40/35 Ground-Glass Joint 47 ea

AH0-1568 Funnel Reservoir, Graduated, 300 mL 47 ea

AH0-3323 Funnel, graduated 500 mL 47 ea

AH0-3324 Funnel, graduated 1000 mL 47 ea

AH0-1569 Vacuum Filter Flask, 1 L, 40/35 Ground-Glass Joint 47 ea

AH0-1570 Aluminum Clamp 47 ea

OrDErInGInFOrMATIOn

Protein Precipitation /Filter PlatePart No. Mfr. No. Description Unit PriceFilter Plate/Precipitation PlatesCE0-7203 6060 Filter Plate PPT, ea Standard 1.2 mL Capacity

CE0-7208 6360 Filter Plate PPT, ea Deep Well 2.5 mL Capacity

All 3M Filtration Products are available from Phenomenex. Call for availability. * 3M and Empore are trademarks of 3M.

See p. 16 for Strata Impact Protein Precipitation Plates.

Universal Resin Membrane Extraction Disk PlatesPart No. Mfr. No. Description Unit Price

CE0-7209 I 6045 (SD) I Universal Resin Plate, I ea I Standard 1.2 mL Capacity

CE0-7210 6345 (SD) Universal Resin Plate, ea Deep Well 2.5 mL Capacity

Disk Plates for Silica-Based ExtractionPart No. Mfr. No. Description Unit PriceStandard Well Extraction Disk Plates, 1.2 mL CapacityCE0-7199 6011 (SD) C2-SD (Ethyl) ea

CE0-7200 6014 (SD) C8-SD (Octyl) ea

CE0-7201 6015 (SD) C18-SD (Octadecyl) ea

CE0-7202 6030 (SD) MPC-SD (Mixed Phase Cation) ea

Deep Well Extraction Disk Plates, 2.5 mL CapacityCE0-7206 6315 (SD) C18-SD (Octadecyl) ea

OrDErInGInFOrMATIOn

DisksforEnvironmentalAnalysis

22 Phenomenex

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SPE VACUUM MANIFOLDS

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See p. 16 for Strata Impact Precipitation Plates.

• Multiplesampleprocessingsavestimeandmoney

• FemaleluerinletsfitallmaleluertippedSPEtubesandcartridges

• InertTeflon®andpolypropyleneflowpath

• Glasschamberallowseasyvisualmonitoring

• Adjustableracksaccommodateavarietyoftesttubes,vialsandflasks

OrDErInGInFOrMATIOn10 - Position Tall-Boy Vacuum Manifold*Part No. Description Unit Price

AH0-7502 I SPE 10-Position Tall-Boy Vacuum Manifold, complete assembly I ea I

AH0-7503 SPE 10-Position Tall-Boy Vacuum Manifold, Glass Chamber ea AH0-7504 SPE 10-Position Tall-Boy Vacuum Manifold, Cover, Gasket and 10 Stopcocks ea

12 – Position Vacuum Manifold*

AH0-6023 SPE 12-Position Vacuum Manifold Set, complete assembly ea

12 – Position Vacuum Manifold Replacement Parts

AH0-6025 SPE 12-Position Glass Chamber ea AH0-6027 SPE Cover, Gasket and 12 Stopcocks ea AH0-6029 SPE Gaskets 2/pk AH0-6031 SPE Vacuum Gauge, Valve and Glass Chamber ea AH0-6037 SPE Collection Rack, including shelves, legs, clips and posts ea AH0-6040 SPE Plate for 13 mm Test Tube ea AH0-6043 SPE Plate for 16 mm Test Tube ea AH0-6042 SPE Plate for Volumetric Flask ea AH0-6045 SPE Plate for Autosampler Vial ea AH0-6046 SPE Plate, Dimple ea AH0-6047 SPE Plate, Base ea AH0-6048 SPE Stopcocks 12/pk AH0-6052 SPE 12-Position Vacuum Waste Container, polypropylene 10/pk

24 – Position Vacuum Manifold*

AH0-6024 SPE 24-Position Vacuum Manifold Set, complete assembly ea

24 – Position Vacuum Manifold Replacement Parts

AH0-6026 SPE Glass Chamber ea AH0-6028 SPE Cover, Gasket and 24 Stopcocks ea AH0-6030 SPE Gaskets 2/pk AH0-6032 SPE Vacuum Gauge ea AH0-6038 SPE Collection Rack, including shelves, legs, clips and posts ea AH0-6041 SPE Plate for 13 mm Test Tube ea AH0-6044 SPE Plate for 16 mm Test Tube ea AH0-6049 SPE Stopcocks 24/pk

General Vacuum Manifold Accessories

AH0-6033 SPE Manifold Needles, polypropylene 12/pk AH0-6034 SPE Manifold Needles, polypropylene 24/pk AH0-6035 SPE Manifold Needles, stainless steel 12/pk AH0-6036 SPE Manifold Needles, stainless steel 24/pk AH0-6050 SPE Drying Attachment for 12-Position manifold ea AH0-6051 SPE Drying Attachment for 24-Position manifold ea AH0-6053 Female Luer Fittings 2/pk AH0-6054 Male Luer Fittings 2/pk AH0-6055 Support Posts for Rack 3/pk AH0-6056 Legs for Cover, black 4/pk AH0-6057 Vacuum Gauge and Valve Assembly ea AH0-6058 Valve Assembly Only ea AH0-6059 Vacuum Gauge Only ea AH0-6060 Retaining Clips 12/pk AH0-6061 Vacuum Manifold Plugs 50/pk AH0-6062 Control Valve, Teflon® 25/pk AH0-6063 Control Valve, Teflon® 50/pk AH0-6064 Teflon® Needles 100/pk AH0-6065 Teflon® Needles 500/pk AH0-7191 Adaptor Caps for 1, 3 and 6 mL SPE tubes, polyethylene, with Luer hole in the top 15/pk AH0-7378 Adaptor Caps for 12 and 20 mL SPE tubes, polyethylene, with Luer hole in the top 5/pk AH0-7379 Adaptor Caps for 60 mL SPE tubes, polyethylene, with Luer hole in the top 5/pk AH0-7907 Adaptor Caps for 150 mL SPE tubes, polyethylene, with Luer hole in the top ea

(1) The 10-position Tall Boy Vacuum Manifold Collection Rack includes 4 plates: one base plate, one dimple plate, one small plate and one large plate and three riser bar legs, along with 12 manifold clips to support the plates. The assembly also includes 10 polypropylene needles, 10 stopcocks and 4 black legs to support the lid when taken off the glass block.(2) The 12-position Collection Rack consists of 3 support posts, bottom plate, 13 mm plate, 16 mm plate, autosampler plate, volumetric plate, and 12 retaining clips. (3) The 24-position Collection Rack consists of 3 support posts, bottom plate, dimple plate, 13 mm plate, 16 mm plate, and 24 retaining clips.

* Manifolds include: Vacuum-tight glass chamber, polypropylene lid with gasket, bleed valve and gauge, stopcock valves, collections racks, polypropylene needles

HavelargeSampleVolumebutneedaSmallBedMass?

useanadaptorcaptoattachanotherSPEtube,whichcanbeusedtoincreasethereservoirsizeforwashingorelutingsolvents.

Adaptor Cap(AH0-7191)

Documents

SPE - Phenomenex · SPE Solid Phase Extraction 3 ... strata-X-CW 33 85 800 0.74 strata-X-AW 33 85 800 0.60 Polar and non-Polar Condition: With methanol Equilibrate: With water