SPE Brochure inside - Pretechbilder.pretech.nu/files/spe_brochurepdf.pdf · 3 608-836-1551 • 800-445-7661 • Solid Phase Extraction Solutions 4 GX-271 ASPEC™SPE is the technique

Solid Phase Extraction Solutions

SPE

SPE_Cover.indd 2 11/7/2008 1:46:41 PM

i 608-836-1551 • 800-445-7661 • www.gilson.com Solid Phase Extraction Solutions ii

Introduction

Gilson offers the broadest array of SPE instruments on the market today, including the

GX-271 ASPEC™, the GX-274 ASPEC™, the SPE 215 and virtually any other Gilson instrument

that accommodates the Code 300 Series Racks. These systems are ideal for pharmaceutical, food &

beverage, clinical, forensic and environmental applications.

Fundamental to Gilson’s automated Solid Phase Extraction (SPE) system is our Mobile Rack and

Positive Pressure technology. The Mobile Rack technology allows you to automate your entire SPE

and Liquid Handling process from the initial condition to the fi nal transfer. Gilson’s SPE instruments

utilize Positive Pressure to ensure more reproducible fl ow rates and recoveries, less sample to sample

contamination and higher precision.

TRILUTION® LH provides an intuitive interface for creating SPE and liquid handling protocols/applications

to condition cartridges, load the sample, wash the cartridge, elute the analyte of interest and transfer the

eluent to a fi nal sample vessel or inject it

directly onto an HPLC or LC/MS system.

Available with a single probe,

multiple probe, or spannable

multiple probe instrument, Gilson

has an SPE and liquid handling

instrument solution to fi t your needs.

Solid Phase Extraction System Features . . . . . . . . . . 1–2

GX-271 ASPEC™ . . . . . . . . . . . . . . . . . . . . . . . . 3–4

GX-274 ASPEC™ . . . . . . . . . . . . . . . . . . . . . . . . 5–6

SPE 215 . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7–8

TRILUTION® LH . . . . . . . . . . . . . . . . . . . . . . . . . 9–10

Code 300 Series Racks . . . . . . . . . . . . . . . . . . . . .11

Automating SPE . . . . . . . . . . . . . . . . . . . . . . . .12

All of Gilson’s SPE and Liquid

Handling instruments are controlled

via TRILUTION® LH software.

TRILUTION LH was designed

with you, the user, in mind. The

graphical interfaces decrease the

learning curve while increasing

your effi ciency. TRILUTION LH allows

you to concentrate on the science,

not the software.

As is the case with all Gilson

instruments, the SPE and liquid

handling instruments are extremely

versatile, modular and robust. Gilson’s SPE instruments are versatile enough to adapt as your science changes and

robust enough to guarantee a low cost of ownership and a workhorse instrument for years to come.

GX-271 ASPECTM with406 Single Syringe Pump

SPE 215 with 849 Multiple Injection Module

1 608-836-1551 • 800-445-7661 • www.gilson.com Solid Phase Extraction Solutions 2

Solid Phase Extraction System Features

GX-274 ASPEC TM with two 406 Dual Syringe Pumps

The 406 Single and Dual Syringe Pumps

deliver Positive Pressure via a gas cylinder

or the syringes. (To achieve more accurate

fl ow rates, use the syringes for the air push.)

Optional peripherals such as injectors,

on-bed solvent reservoirs, three-way valves

and peristaltic pumps extend the capabilities

of your instrument and allow you to tailor

the instrument to meet your specifi c needs.

Compatible with the most common and

widely accepted SPE cartridges and

96-well extraction plates, the Gilson

instruments are capable of utilizing

1, 3 and 6 mL SPE cartridges as well

as 96-well extraction plates.

On-bed rinse stations minimize

carryover and cross-contamination

concerns.

Pressure is constantly monitored and recorded

through pressure sensing modules to immediately

identify a change in performance, cartridge failure

or plugged solvent lines.

Gilson’s newest and most powerful SPE and liquid

handling software yet, TRILUTION® LH allows complete

control of the entire system.

Mobile Rack

technology allows

the user to perform

typical liquid

handling tasks as

well as SPE tasks

from the same

bed layout.

Positive Pressure

elutions guarantee

more reproducible

results, higher

percent recoveries

and more consistent

fl ow rates.


GX-271 ASPEC™

SPE is the technique of

choice for sample clean-

up, sample prep and trace

analyte enrichment due

to its inherent versatility,

selectivity, speed and low

solvent usage. Gilson’s

GX-271 ASPEC is the ideal

solution to automate

solid phase extraction for

pharmaceutical, food &

beverage, clinical, forensic or

environmental applications.

Automated Solid Phase Extraction, Liquid Handling and on-line injection capabilities

608-836-1551 • 800-445-7661 • www.gilson.com

° Operate in both Batch and Sequential mode to increase throughput and efficiency.

° On-bed reservoirs allow access up to four solvents without taking up a valuable rack position.

° Accommodates 1, 3 and 6 mL SPE cartridges.

° Mobile Rack technology enables use as both an SPE instrument as well as a liquid handling instrument.

° Multiple rinse stations with optional rinse pump allows multiple rinsing parameters depending on the application need.

° The 406 Single and Dual Syringe Pumps are equipped with pressure sensing modules that monitor pressure and can be used to handle error conditions such as high cartridge pressure or clogged liquid lines.

° 406 Single and Dual Syringe Pumps enable air pushes from both syringes and external air sources.

° Eluted product can be fractionated by moving to different collection tubes for applications such as buffer or pH step gradients.

° GX Direct Injection Module can be integrated for performing on-line injections onto HPLC or LC/MS systems.

° Positive Pressure achieved via Gilson’s unique and innovative Sealing Cap design.

° Modular design allows the use of Code 20, Code 200, Code 34X and Code 37X Series racks.

Designed with today’s laboratory in mind, the GX Series of

instruments have large cartridge capacities while minimizing the

amount of linear bench space consumed. System performance is

constantly monitored by a pressure sensing module. Carryover

and contamination concerns are minimized through the use of

Gilson’s revolutionary jet wash rinse stations. The combination of

throughput with the ability

to run unattended

makes this instrument

an ideal choice for SPE,

sample prep, sample

clean-up and

GPC/SPE

applications.

1 mL3 mL6 mL

GX-271 ASPEC TM with406 Single Syringe Pump

On-bed injection capabilities

Mobile Racks allow access to both SPE cartridges as well as collection vials

GX-271 ASPEC with on-bed solvent reservoirs

406 Single Syringe Pump

System Overview

Cartridge Types 1, 3, 6 mL Cartridges

Air Push Modes Syringe or Gas Cylinder Valve

On-bed Options Reservoirs, Direct Injection Module

Dimensions (w x d x h) 59.6 x 53.9 x 57 cm (23.5 x 21.25 x 22.46 in)

Cartridge Capacities* 108 – 1 mL cartridges 60 – 3 mL cartridges 45 – 6 mL cartridges*Assumes 3 tray positions used for SPE Mobile Racks.


GX-274 ASPEC™

Gilson’s multiple probe

instruments give you the

ability to maximize your

effi ciency and throughput

by unattended, parallel

processing of samples.

Simply place the samples

on the instrument, and the

SPE cartridges in the

Mobile Racks, then fi ll

any solvent reservoirs.

Next create your sample list.

Then simply press run ...

... its that easy.

Increased throughput through parallel processing

System Overview

Cartridge Types 1, 3, 6 mL Cartridges

Air Push Modes Syringe or Gas Cylinder Valve

On-Bed Options Reservoirs, Four Probe Off Bed Solvent Transfer Port

Dimensions (w x d x h) 59.6 x 53.9 x 57 cm (23.5 x 21.25 x 22.46 in)

Cartridge Capacities* 108 – 1 mL cartridges 60 – 3 mL cartridges 24 – 6 mL cartridges*Assumes 3 tray positions used for SPE Mobile Racks.

608-836-1551 • 800-445-7661 • www.gilson.com

° Four probe Z-arm increases throughput and efficiency.

° On-bed reservoirs allow access of up to four solvents without taking up a position on the tray.

° On-bed transfer ports allow access to two additional large volume solvent reservoirs without taking up any on-bed rack positions. Extend this capability through the use of a VALVEMATE® II.

° Modular design allows the use of Code 146, 200, 34X and 37X Series racks.

° The 406 Dual Syringe Pumps are equipped with four independent pressure sensing modules that monitor pressure on each of the fluid

paths and can be used to handle error conditions such as high cartridge pressure or clogged liquid lines.

° Accommodates 1, 3 and 6 mL SPE cartridges.


° Positive Pressure elution ensures better reproducibility and higher precision.

The GX-274 ASPECTM is a high-throughput, four probe instrument

capable of processing up to four samples in parallel, increasing both the

throughput and effi ciency of your cartridge-based applications. Based

on Positive Pressure elutions this instrument processes samples with

Gilson’s innovative Sealing Cap technology, where each cartridge is

sealed, run and monitored individually. The two 406 Dual Syringe Pumps

allow the user to transfer liquids, process up to four samples in parallel

and monitor each fl uid path individually.

1 mL3 mL6 mL

Collection vessel access

Process four samples in parallel

GX-274 ASPEC TM

° Positive Pressure elution achieved via Gilson’s Sealing Cap technology.

° Eluted product can be fractionated by moving to different collection tubes.

° SPE process can be performed in either Batch or Sequential mode to increase throughput and efficiency.

VALVEMATE® II Valve Actuator

GX-274 ASPEC TM

with two 406 Dual Syringe Pumps


SPE 215 System

What else can you do with

your Gilson SPE system?

Due to Gilson’s modular

design, you have the ability

to match your instrument

to your exact needs.

Peripherals such as

on-bed injectors,

on-bed solvent transfer

ports, on-bed rinse pumps

and on-board solvent

reservoirs allow you

to customize your

instrument to meet

your specifi c needs.

High-throughput Solid Phase Extraction and sample purifi cation

The SPE 215 System is an easy-to-use, powerful, sample

preparation solution for your laboratory. This instrument uses

Gilson’s Integrated Sealing Foot design to achieve Positive

Pressure elutions. Gilson’s multiple probe technology provides

the high throughput capability and the ability to increase

and improve your laboratory’s output and results. Dual Rail™

technology provides accurate and even pressure across all

eight syringes allowing for the selection of four or eight probes.

The integration of these technologies provides you with a

solid SPE solution and results in increased productivity and

effi ciency in your laboratory.

608-836-1551 • 800-445-7661 • www.gilson.com

System Overview

Cartridge Types 1, 3 mL Cartridges, 96-well extraction plate

Air Push Modes Syringe or Gas Cylinder from Solenoid Valve

On-bed Options Reservoirs, On-line Injection via 849 Multiple Injection Module

Dimensions (w x d x h) 97.8 x 61 x 64 cm (38.5 x 24 x 25.2 in)

Cartridge Capacities* 288 – 1 mL cartridges (tabless) 144 – 3 mL cartridges 3 – 96-well extraction plates*Assumes 3 tray positions used for SPE Mobile Racks.


° Accommodates 1 and 3 mL cartridges or 96-well extraction plates.

° Can be operated in both Batch and Sequential mode to increase throughput and efficiency.

° Four or eight probe configurations to increase throughput and efficiency.

° Positive Pressure ensures better reproducibility and higher precision.

° Positive Pressure achieved via Gilson’s Integrated Sealing Foot design for both cartridges and plates.

3 mL 1 mL 96-well extraction plates

SPE 215 with 849 Multiple Injection Module

SPE 215 Mobile RacksGilson’s Integrated Sealing Foot design

9 608-836-1551 • 800-445-7661 • www.gilson.com Solid Phase Extraction Solutions 10608-836-1551 • 800-445-7661 • www.gilson.com

TRILUTION® LH Software

Complete control for automated

Solid Phase Extraction (SPE)

applications.

Our latest software

package, TRILUTION® LH,

is Gilson’s most

easy-to-use and most

powerful SPE and liquid

handling software yet.

Designed with the user

in mind, the software

is extremely fl exible

allowing you to focus

on the science rather

than the software.

The complete software solution for your Liquid Handling and SPE applications

This intuitive software offers

easy-to-use capabilities for the

every day user.

° User-friendly graphical interfaceminimizes the learning curve while maximizing efficiency.

° One software package to control all liquid handling and SPE instruments maximizes the utility of the hardware.

° Drag-and-drop functionality enables you to quickly and easily set up instrument configurations and define bed layouts.

° The Application Run screen provides complete control over your run; from set up to application run, to simulation and the modification of your run.

° Import/Export functionality allows you to transfer methods from one system to another quickly and easily.

° Over 70 Gilson-supplied tasks make programming methods and creating applications quick and easy.

Wait TaskTips Rinse Task

Mix With Tips Task

SPE Evaporate

Task

SPE Wash Task

SPE Load Task

Dispense Random

Task

Derivatize Task

Add Task

SPE Elute Task

SPE Dry TaskSPE Condition

Task

° Sample list editor makes building your application easier than ever with spreadsheet data entry and functionalities such as copy down and increment.

° Graphical task pages organize data entry, allowing the user to quickly create and modify task properties.

° Simulation mode allows the user to test and verify methods and applications prior to running the instrument.

° Dynamic, graphical property pages ensure quick and easy data entry and variable creation, while eliminating the need to create long variable lists giving you the ultimate flexibility with minimal effort.

° Manual Control pages allow you to perform routine tasks, troubleshoot the instrument and set up the instrument prior to running your applications.

Simulation in Application Run screen

Method in Method BuilderM h d i M h d B ild

Bed Layout in Method Builder

SS Dispense Task property page

GX-271 Manual Control page

Custom Task Builder

ProcessorIntel® Pentium® 4 (2.8 GHz)

Fixed Drive80 GB, Available space: 4 GB (6 GB at install)

Computer RecommendationsVideo Card1024 x 768 with 24 bit True Color

RAM1 GB

Additional DriveDVD/CD-RW

Serial Ports 2

Operating SystemMicrosoft® Windows® XP Professional SP2 or later, Microsoft® Windows® 2000 SP4

Microsoft, Windows, Word, and Excel are either registered trademarks or trademarks of Microsoft Corporation in the United States and/or other countries


Whether looking for high-

throughput, pre-injection clean-

up or simple sample prep before

injection, users can customize

their Gilson-based systems with

the Code 300 Series Mobile Racks.

The robustness of Gilson’s liquid

handlers when combined with

Mobile Rack technology provides

a customized solution for any of

their SPE/purifi cation needs.

Code 300 Series Racks

The Code 300 Series Racks now allow virtually all of Gilson’s existing

instruments to perform SPE, fi ltration and liquid handling tasks.

Combine all the features and functionality of Gilson’s liquid handlers

and the time tested Mobile Rack technology to customize your

instrument to your specifi c application needs.

° The Code 300 racks accommodate 1, 3 and 6 mL cartridges.

° The Code 300 Series Racks can be used on any Gilson instrument that accommodates a Code 200 Series Rack, effectively transforming automated liquid handling instruments into solid phase extraction systems.

° Compatible with single probe, multiple probe or spannable multiple probe instruments.

SPE Process

Analyte Interferences

Conditioning Loading Sample Washing Eluting

traction Solutionsxtraction Solutions

Extend the capabilities of your Liquid Handling instrument

Why automate SPE?

Automating SPE increases throughput

and improves reproducibility, while

minimizing the exposure to harmful

chemicals. Gilson’s instruments are

designed to be workhorse instruments

that can be set up quickly. The

application can be started while the

researcher works on other tasks and

the instrument will fi nish effortlessly.

This minimizes the cost of labor

typically associated with manual

SPE processes, and allows SPE to

be performed without a researcher

present. With single, four probe and

eight probe parallel processing units,

you decide which instrument provides

the throughput needed for your

application.

Code 306 and 301 Racks

System Overview

Cartridge Types 1, 3 and 6 mL Cartridges.

Instrument All instruments capable of using Code 200 Series RacksCompatibility

Rack Code 300 Rack Code 300: 1 mL cartridge w/ 96-well deep microplate collectionOptions Rack Code 301: 1 mL cartridge w/ 96-well shallow microplate collection Rack Code 302: 1 mL cartridge w/ 48 12x32 mm vials (2 mL) Rack Code 303: 3 mL cartridge w/ 48 12x32 mm vials (2 mL) Rack Code 304: 3 mL cartridge w/ 48 12x75 mm tubes (5 mL) Rack Code 305: 3 mL cartridge w/ 32 15x45 mm vials (4 mL) Rack Code 306: 6 mL cartridge w/ 20 12x75 mm tubes (5 mL) Rack Code 307: 6 mL cartridge w/ 20 15x45 mm vials (4 mL)

GX-271ASPEC

GX-274ASPEC

SPE215

Gilson’s Automated SPE SolutionsDesigned to Run Unattended

(20+ hours / day)

SPE Samples Processed per Day(Assuming a 10 min run time)

Sam

ple

s P

rocessed

480

960

120

Gilson, Inc. World Headquarters | 3000 Parmenter Street | P.O. Box 620027 | Middleton, WI 53562-0027, USA | Tel: 608-836-1551 or 800-445-7661 | Fax: 608-831-4451

Gilson S.A.S. | 19, avenue des Entrepreneurs | BP 145, F-95400 VILLIERS LE BEL, FRANCE

www.gilson.com | [email protected] | [email protected] | [email protected]

©2008 Gilson, Inc. | LT303017-05 | Printed in the USA. | Specifications subject to change without notice.

SPE_Cover.indd 1 11/7/2008 1:46:39 PM

Documents

SPE Brochure inside - Pretechbilder.pretech.nu/files/spe_brochurepdf.pdf · 3 608-836-1551 • 800-445-7661 • Solid Phase Extraction Solutions 4 GX-271 ASPEC™SPE is the technique