SP-400-M1/SP-403K-M1 INSTRUCTIONS - MS Wil GmbH · PDF fileSP-400-M1/SP-403K-M1 INSTRUCTIONS Packing limit of the pressure vessel is 9000 ... 3.3 Place the SP-116 (FIG 9) nut and ferrule

PACK YOUR OWN CAPILLARIES FOR HPLC AND LC-MS

SP-400-M1/SP-403K-M1 INSTRUCT IONS

revision 3-15-15

SP-400 CUTAWAY

TABLE OF CONTENT

SP-400-M1 AND SP-403-M1 KIT COMPONENTS PAGE 2COMPONENT ASSEMBLY DIAGRAM PAGE 3COMPONENT PHOTO LIBRARY PAGE 4GENERAL INFORMATION, SAFETY & CARE PAGE 5ASSEMBLY & CONNECT TO PRESSURE SOURCE PAGE 6COLUMN ASSEMBLY PAGE 8SLURRY PREPARATION PAGE 10ATTACHMENT OF COLUMN PAGE 10PACKING THE COLUMN & LC-MS TIPS PAGE 12COLUMN REMOVAL PAGE 13PARTS AND ACCESSORIES LISTING PAGE 15- 20

NanobaumeTM is a Trademark of Western Fluids Engineering & Mfg, LLCInconel® is a Registered Trademark of Inco Alloys International, Inc.Kel-F® is a Registered Trademark of the 3M CompanyPEEKsilTM is a Trademark of SGE International Pty. Ltd.PEEKTM polymer is a Trademark of Victrex PLCPolySULFOETHYL ATM, PolyCAT ATM, PolyHYDROXYETHYL ATM, and PolyPROPYL ATM are trademarks of PolyLC Inc.Teflon® and Tefzel® are Registered Trademarks of E.I. du Pont de Nemours and Company

© Western Fluids Engineering and Manufacturing, LLC

2

Nanobaume™ Kit ComponentsUse the SP-400-M1 and SP-403K -M1 NanobaumeTM kits to pack micro and capillary

columns of various lengths and of outer diameters . The compact design seals effectively at high pressure, up to 9000 psi*. The following items are included in the SP-400-M1:

PART # IMAGE SP-1333 G

SP-014 L

SP-01 5 M

SP-400-070-07 I

SP-085 J

SP-116116 E

SP-400-01 A

SP-400-02 B

SP-400-05 C

VL-002 D

DESCRIPTION FERRULE, PEEK, FOR 1/32” OD COLUMNS (QTY 5) STAINLESS STEEL NUT, 10-32 (for SP-015 only) FERRULE, KEL-F®, FOR 360 MICRON OD (QTY 5) O-RING, FFKM Perfluoroesalstomer

PTFE STIR BAR, 5MM X 2MMPRESSURE CONNECTION 10-32 MALE FOR 1/16" TUBERESSCAP LOCK, SS316

CAP SEAL, SS316PACKER BODY, SS316

GLASS VIAL, 2ML, 12X32MM, BOROSILICATE

The SP-403K-M1 includes the SP-400-M1, in addition to the following accessories:

PART # IMAGE DESCRIPTION

SP-087 N SP-046 O SP-050 P SP-055 Q SP-058 R VL-002X D CE-005-M1 T

SP-400-M1/SP-403K-M1 INSTRUCTIONS

Packing limit of the pressure vessel is 9000 psig with liquid media. Use of fused silica capillary and adapters will reduce the working pressure of packer to 6000 psig.

*

SP-403K-M1 nobaumeTM Startup Kit

SP-400-M1 NanobaumeTM Assembly

SP-134SP-130SP-125

FERRULE FOR 1/16" COLUMN CONNECT SS NUT FOR 1/16" COLUMN CONNECTVIAL INSERT FOR 2ML VL-002 VIAL

HFK

SP-110 S

PRESSURE GAUGE, 0-10000 PSI, SS316TUBING, 0.020” ID X 1/16” OD X 10CM, SS316 (QTY 2) SHUTOFF VALVE, WITH BOTTOM VENT TUBING, 0.020” ID X 1/16” OD X 5 FEET, SS316 PRESSURE GAUGE GAUGE TEE FOR 1/16” OD TUBING GLASS VIAL, 2ML, 12X32MM BOROSILICATE GLASS PK/10 HIGH PRESSURE PACKING KIT, includes CE-003, CE-006, plus other items, see main brochure for details

TANK ADAPTER, 1/4" MALE NPT TO 1/16" TUBE

3

C O M P O N E N T S A N D A S S E M B L Y

Cap Lock

10-32 Nut

Ferrule

Cap Seal

Stir Bar

Glass Vial

O-Ring

Packer Body

SP-050 Valve with bottom vent

Pressure Source

Pressure Vent(Bypass)

SP-087 Pressure Gauge with SP-058

Gauge Tee

Direction of Pressure Flow

SP-400 Packing Unit

CE-003 End Fittingretains silica bed

while packing

Complete Column with

CE-003 End Fittings

SP-403K-M1 PARTS ASSEMBLY DIAGRAM

SP-400 -M1 PARTS ASSEMBLY

SP-055

SP-046

SP-400-01

SP-130

SP-133 or SP-134

SP-400-02

SP-085

VL-002VIAL INSERT SP-125 not shown

SP-400-07

SP-400-05

NOW 10-32 FEMALE THREAD

SP-116

SP-130 NUT with SP-133 or SP-134

Tank or Pump

4

COMPONENTS OF THE SP-400-M1 AND SP-403K-M1

A

N

01

Items A-M above are included in our base unit, the SP-400-M1 Nanobaume. The SP-403K-M1 Startup Kit includes all the components of the SP-400-M1, as well as the additional packing accessories illustrated below (items N-T).

T

B

CAP LOCK, SP-400-01

CAP SEALSP-400-02

C BODY, SP-400-05

D VIAL VL-002

E PRESSURE CONNECTIONSP-116

F COLUMN CONNECIONSP-130

G FERRULEFOR TUBE 1/32"SP-133

H FERRULEFOR TUBE 1/16" SP-134

I O-RING SP-400-07

J PTFE STIR BAR SP-085

K VIAL INSERT SP-125

L COLUMN NUT SP-014

MFERRULE, 360UM TUBE SP-015

PRESSURE GAUGE SP-087

O SS TUBE 1/16"x .020" x10CMSP-046

P VALVE PRESSURE RELIEF SP-050

Q SS TUBE 1/16"x .020"x 5FTSP-055

R TEE, PRESSURE GAUGESP-058

S REGULATOR ADAPTER SP-110

CE-005-M1 COLUMN ADAPTER KIT SEE MAIN BROCHURE FOR COMPONENTS CONTAINED.

1/32" OD COLUMN END FITTINGS CE-003,2 EA1/16" OD COLUMN END FITTINGS CE-006, 2 EA1/32" SS SCREEN .5UM, 2 EA1/16" SS SCREEN .5UM, 2 EAFUSED SILICA 360UM OD X 150UM ID, .5MTR PEEKSIL, 1/32", 100UM ID X 100MM LONG PK/2 PEEKSIL,1/16",150UM IDX 150MM, NOT SHOWN 2 EA FERRULE, WITH SS RING 1/32" PEEK, 2 EA FERRULE, WITH SS RING 1/16" PEEK, 2 EAMALE NUT, SS 10-32 , 1 EA ADAPTER SLEEVES SET FOR F.S. , 1 SET

1. GENERAL INFORMATION

1.1 The Nanobaume is a compact stainless steel unit designed to pack capillary columns with a silica

bed at high pressure. Its unique sealing design permits rapid assembly, facilitating quick packing

changeover. Simple hand tightening the pressure vessel will seal up to 9000 psig with liquid media,

3000 psig with gas. No tools are required to operate the packing unit, although a set of adjustable

wrenches is useful for initial setup and column assembly. SP-400-M1 and SP-403K-M1 kits include

connections for 360um, 1/32" OD , 1/16" outside diameter (OD) columns, but the NANOBaume will

accept small OD column diameters using the TS-015 sleeve kit. LC-MS spray tips may also be packed.

Component materials include Kel-F, PEEK, 316SS, 304SS, Borosilicate glass, ETFE, and FFKM

perfluoroelastomer. The vial insert (SP-125) supplied with the SP-400-M1 will accept 2 ml (12x32mm)

glass vials. Other inserts are available, (please inquire)

2 PRODUCT SAFETY AND CARE

2.1 The NANOBaume is not for use under Supercritical Fluid conditions. Do not exceed the publishedpressure limits (9000psig liquid/3000 psig gas). Always operate with a safety shield in place. When

packing with liquid media, first flood the Nanobaume and empty column with solvent. If using the TS-

015 sleeve adapters or SP-015, SP-014 Ferrule/Nut for the capillary columns , then pressure limit is

reduced to 6000psig.

CAP LOCK, SP-400-01 O RING, SP-400-07 CAP SEAL, SP-400-02 BODY, SP-400-05

FIG 1 FIG 2 FIG 3 FIG 4

2.2 Hand tighten the cap lock (SP-400-01, FIG 1) on the packer body (SP-400-05 ,FIG 4) and do not

overtighten, as galling may occur between the stainless steel surfaces; the O-ring seals at high pressure.

Scratches, nicks or dents on the sealing surface of the Cap seal (SP-400-02, FIG 3), Nanobaume body

5

(SP-400-05, FIG 4) or O ring (SP-400-07, FIG 2) may impede sealing performance. As the Nanobaume

is primarily 316 SS; ethers, concentrated oxidizing acids are not recommended. (i.e. Hydrochloric acid

at greater than 10%) If small concentrations of aggressive solvents are used during packing, it is

highly recommended that the unit be flushed with a weak solvent after use.

3. ASSEMBLY AND CONNECTION TO PRESSURE SOURCE

PLEASE NOTE; THE FOLLOWING INSTRUCTIONS ARE WRITTEN TO INCLUDE COMPONENTS

FROM THE SP-403K-M1 STARTUP KIT. CUSTOMERS WHO PURCHASED THE SP-400-M1 BASE UNIT

MAY PURCHASE THESE PACKING ACCESSORIES SEPARATELY.

3.1 Remove the SP-400-M1 (FIG 5) and SP-403K-M1(FIG 6) components from the shipping container.

Components shown on Page 4

FIG 5 FIG 6

NANOBAUME NANOBAUME ACCESSORY KIT

SP-400-M1 KIT SP-403K-M1 KIT

3.2 Fit the Gauge Tee (SP-058) with the pressure gauge (SP-087). Wrap the ¼” NPT threads 3-4 times

with teflon tape (TTB-50) Wrench tighten.

6.


Gauge tee, SP-058 Gauge,SP-087 PRESSURE FITTING , SP-116 SS TUBE, SP046

3.3 Place the SP-116 (FIG 9) nut and ferrule on the end of the SP-046(FIG 10) stainless steel tubing,

tighten into the side port of the Nanobaume until tube is secure. The SP-116 is reusable as the ferrule

does not swage permanently onto the tube. Connect the free end to the gauge tee (SP-087) and wrench

tighten to secure the tube. Note: if a torque wrench is purchased, SP-162 torque to 10 Inch-pounds. If

not, finger tighten the nut (Clockwise direction), then wrench tighten ¼” turn then check if leaks under pressure. If leaks occur, reduce pressure, ¼ turn more and repressurize , if leaks, repeat previous steps. Use this technique for tightening all Stainless steel nuts in this publication.)

FIG 11

Pressure relief Valve with bottom drain, SP-050

3.4 Using the second piece of SS tube (SP-046), Connect one end to the Pressure gauge Tee (SP-087),

and other end to the Pressure vent valve (SP-050, FIG 11). Wrench tighten both until tubes are secure

using technique described in 3.3. The assembly should look like Fig 12.

7.

FIG 12 FIG 13 FIG 14

SET UP SS TUBE, SP-055 REGULATOR ADAPTER SP-110

3.5 Connect the uppermost port of the Vent valve (SP-050, ) to the pressure source. First use the

Long piece of SS tube (SP-055, FIG 13) connect using fitting provided on the vent valve (upper port, right

side of FIG 11) tighten to secure the tube. Take the SP-110, FIG 14 Pressure Regulator Adapter ,1/4"

male NPT end and apply teflon tape to threads wrapping 3-4 times around then thread into the port of

the pressure regulator(not provided). Take the free end of the tubing and place into the other end of

the pressure regulator adapter , and tighten until the tube is secure. If the HPLC pump is being used

for the high pressure line, connect directly to pump using a 10-32 female thread union (SP-095 not

provided)

4. COLUMN ASSEMBLY

4.1 If fused silica tube will be used, cut the fused silica to length using an appropriate cutting tool . Aprecision cutter will ensure a square end without fragmentation of the column end (SP-019, not provided). Secure the column end fitting CE-003 ,( FIG 15), on the exit end of the column using an

appropriate adapter sleeve,(TS-015,FIG 20) with Nut, ( SP-130, FIG 22) and Ferrule,( SP-133, FIG 23).

The ferrule –nut- sleeve fused silica combination should look like figure 21. Tighten until fingertight

and then 1/4” turn more to secure the column to the end fitting. Be sure to push the column body into

the end fitting to eliminate a void between the column end and the web of the union.


COLUMN END, CE-003 SS FERRULE , SP-092 SS NUT , SP-102 SS UNION, SP-089

4.2 Assemble the Column end fitting columns 1/32” OD or smaller, use the CE-003 From the CE-003 kit,

FIG 23.5NUT , FERRULE ASSEMBLYSP-130/SP-133

8.

place a 0.5um screen (SP-088, not shown) into the Micro Union (SP-089, FIG 15). Ensure that the screen

is set in flat against the bottom web of the union. Place the 1/32 inch OD tube or fused silica ( adapted

to 1/32” OD ) thru the nut (SP-102, FIG 17) and ferrule (SP-092, FIG 16) These are provided with the

union. Insert the column body into the union and while exerting pressure on the tube into the union,

tighten the nut using two wrenches. Use similar procedure for the CE-006 kit for 1/16” od column

bodies, follow steps 4.2 .


4.3 The nut ferrule fused silica should look like fig 19. The sleeve on the fused silica will bring it up to

an outside diameter of .032”(1/32") to fit the column end fittings (CE-003) Note; that the use of the

fused silica will reduce the pressure rating of the column and packer to 6000 psig.

FIG 19, FUSED SILICA WITH FIG 20 ,TS-015 ADAPTER SLEEVE FIG 21, PEEKSIL WITH SP-130/SP-133FERRULE SP-015, NUT SP-014

SP-130 SS NUT SP-133 FERRULE SP-015 FERRULE 360um SP-014 SS NUTuse SP-133 or SP-134 ferrule use sp-130 nut use SP-014 nut use SP-015 ferrule

9.

4.4 LC-MS tips are inserted into the appropriate ferrule, and/ or sleeve depending on OD, and do not

require end fittings as the tips are fritted already.

5. SLURRY PREPARATION

5.1 PLEASE NOTE: AS CAPILLARY PACKING IS AN EXPERIMENTAL SCIENCE, SOME

TESTING WITH VARIOUS SOLVENTS OR COMBINATIONS MAY BE REQUIRED FOR

OPTIMAL PACKING.

5.2 Weigh 100 mg of bulk packing material into the 2ml vial (VL-002, FIG 26) Add 1.5ml of appropriate

solvent, or buffer (Hexane for normal phase, Propanol or acetonitrile for reversed phase, weak buffer

for ion exchange) to the vial. Add the stir bar ( SP-085, FIG 27)

FIG 26 FIG 27 FIG 28

VIAL VL-002 STIR BAR SP-085 VIAL INSERT SP-125

5.3 Place the vial on a magnetic stir plate. Allow the slurry to stir for 5 minutes or until the stationary

phase is well wetted. Once the bonded phase is wetted insert the vial into the packer body cavity using

the vial insert (SP-125, FIG 28) provided to hold the vial in place

10.


FIG 33

FUSED SILICA 360um PEEKSIL 1/32 1/16” OD FERRULE SP-134

SLEEVED FUSED SILICA

SP-014/SP-015

nut SP-130/ ferrule SP-133 nut SP-130/ferrule SP-134

6.1 With the correct adapter, most column or tubing with an outer diameter of 1/32inch or less can be attached

to the Nanobaume(FIG 29 OR 30) using the nut ,SP-130 ( FIG 22)And ferrule, SP-133 (FIG23). Larger 1/16” OD

columns can be attached FIG 31, using the SP-130 (FIG 32 )nut and the Ferrule SP-134 (FIG 32) ferrule

combination to the Nanobaume . In the SP-400-M1 kit, three adapter ferrules are provided. For 360 um OD fused

silica use the SP-014 nut with the SP-015 ferrule. For 1/32inch OD tube use the SP- 130 nut with the SP-133

ferrule. For 1/16 inch OD tube use the SP-130 nut with the SP-134 ferrule. These connections are a convenient

way to connect column bodies to the Nanobaume. If the fused silica tube that has a different OD than 360, use an

adapter sleeve from the TS-015 kit and the SP-130/SP-133 combination. The adapters in the TS-015 kit will adapt

fused silica columns of OD of 125 to 495 micron to a new OD of 1/32” to fit the SP-130/SP-133 ,( FIG 33)

NOTE: USE OF FUSED SILICA WILL REDUCE THE OPERATING PRESSURE LIMIT TO 6000 PSIG

6.2 Unscrew the Cap Lock (SP-400-01,FIG 1) counterclockwise and remove the Cap Seal (SP-400-02, FIG

3) if not accomplished already.

FIG 34COLUMN ON NANOBaume

SP-133 nut ferrule assembly,

11.

6. ATTACHMENT OF COLUMN TO NANOBAUME

FIG 34 FIG 35

COLUMN INSTALLED NANOBAUME SETUP

IN CAP SEAL

6.3. Using the appropriate ferrule sized to the column OD, insert the column body open end through

the ferrule and the nut (refer to section 6.1) Tighten the nut only finger tight into the threaded port of

the Cap seal (SP-400-02 FIG 34) column will be adjusted in the next step.

6.4 With the column in place, insert the Cap Seal onto the Nanobaume body ,( FIG 33). Insure that the

“O” ring is in place (figure FIG 2) Thread on the Cap Lock (SP-400-01, FIG 1) Hand tighten only as the O-

ring under pressure forms the seal, not the fit of the cap lock and seal. DO NOT OVERTIGHTEN Push the

column down into the slurry vial until it meets the bottom of the vial, then withdraw the column by 1/8

inch or far enough to clear the rotation of the stir bar at the bottom of the vial.

6.6 Tighten the Nut SP-130 (or the SP-014) to firmly secure the column in place ) Use two wrenches and

the wrench flats on the Cap Seal to further tighten the nut. Overtightening the nut may embed the

ferrule in the Cap seal port. If this problem occurs, take out the nut, and push the ferrule from the

bottom side. Use a straight piece of 1/6” ss tube, or 1/32 Ferrule extraction tools are available. (contact

the factory)

7.0 PACKING THE COLUMN and LC-MS TIPS

NOTE: IF PACKING WITH LIQUID MEDIA, THE PACKING SOLVENT USED SHOULD BE THE SAME AS THAT

USED TO SUSPEND THE SILICA SLURRY IN THE VIAL. IF PACKING WITH GAS , USE OF NITROGEN OR

HELIUM IS RECOMMENDED.

7.1 Place the assembled Nanobaume and column behind a safety shield and onto a magnetic stir

plate(NOT PROVIDED) .The setup should approximate the setup shown ( FIG 35) Turn on the stir plate.

12.

With the bypass on the SP-050 (FIG 11) high pressure valve still open, slowly turn on the pressure

source. The system pressure will vent through the bypass until the bypass is closed.

7.2 Slowly close off the bypass (clockwise turn) on the SP-050 valve handle to gradually increase

pressure to the Nanobaume. If packing with liquid media, first flood the system and column with

solvent. A small amount of air will be trapped inside the Nanobaume body and will be reduced in size by

the pressure and not impact the packing process. Note: do not increase the packing pressure quickly as

this might break the glass vial with differential pressure.

7.3 Set the flow rate to reach a stable pressure by adjusting the nitrogen tank regulator valve, or the

liquid pressure source. As the slurry packs into the column, the pressure will rise. Take care to reduce

the flow rate to ensure that the pressure limits of the end fitting, column to Nanobaume connection or

the SP-400 pressure Vessel are not exceeded. . Flowrate and resulting pressure are interdependent.

(More flow, higher the back pressure generated with a given bed length).

7.4 Monitor the pressure gauge (SP-087, FIG 8), watching for a stable pressure level. Although the

column is packed once this stable pressure is reached, the silica bed is not yet stabilized. Allow the

pressure source to maintain the column pressure for at least 60 minutes, longer is some cases up to

8 hours to reach a stable pack for longer columns.

7.5 For packing of the LC_MS Tips use gas and pack at pressure not exceeding 3000 psig. Tips pack

quickly and visual confirmation is used in lieu of stable pressure reading.

8 COLUMN REMOVAL AND COMPLETION

8.1 Shut off the pressure source and relieve the system pressure by gradually opening the bypass on the

SP-050 valve (counter-clockwise turn).

8.2 Remove the column from the Cap Seal, loosening the nut counterclockwise 2 full turns. Carefully

remove the column from the cap seal. Note , removal of the column without equalizing pressure in all

parts of the NANObaume setup, might cause the packing material to extrude from the open end of the

column. Please refer to FIG 36 , top column is fused silica column with sleeves, middle is the 1/32”

PEEKSil column, and the bottom is a 1/16” SS body. These columns are not shown with the SS screens,

as they are to small to photograph.

13.

FIG 36

COLUMNS READY FOR ENDS

ATTACHEMENT, SCREENS NOT SHOWN

8.3 Install a suitable column end fitting onto the open end of the column (SECTION 4) (FIG 36)

8.4 The newly packed column may be attached to a pump to flush with suitable solvent to waste. The

column is now complete and ready for use.

NOTE: WE OFFER NO GUARANTEE REGARDING COLUMN TO COLUMN PACKING REPRODUCIBILITY,

COLUMN EFFICIENCY (THEREOTICAL PLATES ACHIEVED), OR COLUMN SELECTIVITY OR TAILING FACTORS,

CAPILLARY COLUMN PACKING IS AT THIS STAGE, A HIGHLY EXPERIMENTAL TECHNIQUE.

WARNING; PACKING A HIGH PRESSURE CAN BE HAZARDOUS, DO NOT USE AT PRESSURE ABOVE

RECOMMENDED LEVELS (9000 PSIG LIQUID, 3000 PSIG GAS) NOT FOR SFC(SUPERCRITICAL FLUID)

column packing with CO2.

14.

PARTS AND ACCESSORIES

Connection of the Nanobaume to a pressure supply source requires an adapter and

several lengths of high-pressure tubing. Most of the replacement parts are found

included in the kits shown in this brochure.

Tubing and fittings

Part Numbers Description

SP-013 Ferrule for 1/32" OD columns (use with the SP-014 nut)

SP-014 Nut SS, 10-32 male threads, Use SP-013, SP-015 ferrules only

SP-015 Ferrule for 360um OD (Use with SP-014 nut only)fused silica

SP-116 Pressure source connection, 10-32 male SS

SP-130 Nut SS , 10-32 male threads, use SP-133 , SP-134 ferrules only

SP-133 Adapter ferrule for 1/32 OD tube, use with SP-130 nut

SP-134 Adapter ferrule for 1/16" ID tube, use with SP-130 Nut.

SP-046 Tubing, SS 1/16" ODx.020" ID x 10cm long

SP-055 Tubing , SS 1/16" OD x .020 " ID , 5 ft long

SP-051 Adapter, 1/14" NPT to 1/16" OD tubing, for adapt pressure regulator

Nanobaume Parts

SP-400-01 Cap lock, SS with titanium nitride coating

SP-400-02 Cap Seal, SS

SP-400-07 "O" Ring, FFKM, perfluoroelastomer

SP-058 Gauge Tee, for 1/16" line and 1/4" NPT female gauge connection.

SP-085 Stir Bar, PTFE Coated, 5x2mm

SP-087 Pressure gauge, 0-10,000 psig, SS, 1/4" male NPT connection.

SP-050 Three way dump valve, for 10,000psig line, 1/16" inch fittings included.

SP-125 Vial insert, Kel-F for 2l 12x32mm flat bottom vials

15

SP-126 Vial insert, Kel -F for 1.5-1.7ml centrifuge vials

VL-002x Glass vials, 2ml, borosilicate glass , 12x32mm, Pack of 10 vials

Low pressure accessories (less than 3000 psig gas or liquid)

SP-098 "O" Ring, Viton

SP-029 Pressure gauge for 0-3000 psig, SS, 1/4" NPT male connection

SP-030 PEEK green 1/16" od x .030" ID tubing 5 ft length

Column End fittings Parts

SP-088 Screen SS, 1/32" diameter with 0.5um sold each

SP-096 Screen SS, 1/16” diameter with 0.5um , sold each

SP-089 1/32 SS micro-union including nuts and ferrules

SP-092 Ferrule, SS, 1/16” for SP-095 nion

SP-103 Ferrule, 1/32" for the SP-089 union

SP-102 Nut for the above SP-089

SP-095 1/16" SS Micro-union including nuts and ferrules

SP-096 Screen, SS 1/16" micro union, 0.5um for the SP-095

SP-135 SSI Ferrule 1/16" for SP-050

SP-136 SSI Nut, 1/16" for High Pressure SP-050

SP-137 Ferrule SS, OD 1/16" for the SP-058

Miscellaneous items

SP-160 Three way SS valve 1/16” fittings, 2500psig limit

SP-161 Teflon tape for NPT threaded parts

SP-019 Precision Cutter for Fused silica

SP-162 Torque wrench 1/4inch Hex, 10 inch- pounds

SP-110 Pressure Regulator, Adapter, ¼” NPT threads to 1/16” tube,

16.

Kits

CE-003 Column end fitting kit, for 1/32 OD tube

CE-006 Column end fitting kit, for 1/16” OD tube

CE-005 Fitting kit for column adapters, 1/32 inch OD and smaller bodies

CE-007 Fitting kit for column adapters, 1/16” inch OD column bodies

SP-400 mod 1 Nanobaume post 03-01-2015

SP-401 mod 1 Nanobaume Kit post 03-01-2015

TS-015 Adapter kits for increasing OD to 1/32 inch from fused silica OD of 125 to 495 micron

Fused silica Tubing Adapters (PEEK, 6000psig rated)

Catalog number Description (For Fused silica of OD listed below)

SP-068 SLEEVE, PEEK, 1/32 inch OD, adapting fused silica tube of 70-110 um OD (Outside

dimension)

SP-069 SLEEVE, PEEK, 1/32 inch OD, adapting fused silica tube of 125-165 um OD








PEEKSIL Empty column bodies 1/32" OD

Catalog number description pressure rated

SP-031 25um ID x 1/32" OD, 50mm long, pack of 2 10,000 psig




17.












Use CE-003 for column end fittings

PEEKSIL Empty Column Bodies 1/16" OD

Catalog number description pressure rated

SP-150 100um ID x 1/16" OD, 150mm long, each 10,000 psig






Use CE-006 for column end fittings

1/32”, 1/16” OD ; Other PEEKSil ID’s and Lengths are available, in precut lengths; Please inquire

Bulk Bonded Phase Supports, Bulk sold by the gram

SP-008 Strong Cation Exchange, PolySulfoethyl A 5u, 300A

SP-004 Weak Cation Exchange, PolyCAT A 5um, 1000A

SP-005 Hydrophillic Interaction , (HILIC) PolyHYDROXYethyl A, 5um, 300A

18.

SP-007 Hydrophobic Interaction (HIC) Polypropyl A, 5um 300A

SP-006 C-18, 5um, 300A

SP-002 C-18, 5um, 100A , Hydrophillic endcap

SP-001 C-18, 5um, 100A

Fused Silica Column Bodies and transfer tubing

Deactivated Fused Silica Tubing , sold in 1 meter length

SP-009 50um ID x 363 um OD Deactivated methyl

SP-010 100um ID x 365um OD Deactivated methyl

SP-011 250 um ID x 350um OD, Deactivated Methyl

Un-Deactivated Fused Silica, sold in 1 meter length

SP-059 5um ID x 363um OD, Un-Deactivated

SP-060 10um ID x 363um OD , Un-Deactivated



SP-063 75um ID x 363umOD, Un-Deactivated


SP-065 200um ID x x363um OD, Un-Deactivated

SP-066 250um ID x 363 um OD, Un-Deactivated

Fused tubing Cutter

SP-019 Precision Tube Cutter for fused silica

.

19.

Nanobaume Operating

Instructions REVISION 3-15-15

Western Fluids Engineering LLC

Lake Elsinore CA 92530

20.

Documents

SP-400-M1/SP-403K-M1 INSTRUCTIONS - MS Wil GmbH · PDF fileSP-400-M1/SP-403K-M1 INSTRUCTIONS Packing limit of the pressure vessel is 9000 ... 3.3 Place the SP-116 (FIG 9) nut and ferrule