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Product Information Solus Half Fraser Broth (FBH001, FBH002, FBH005) Formulation Grams/litre Peptone mixture 15.0 Yeast extract 5.0 Aesculin 1.0 Disodium hydrogen phosphate 9.6 Potassium dihydrogen phosphate 1.35 Sodium chloride 20.0 Lithium chloride 3.0 Acriflavine 0.0125 Nalidixic Acid 0.01 Directions Weigh 55 grams of powder and dispense into 1 litre deionised water. Allow to soak for 10 minutes, swirl to mix and sterilise at 121 o C for 15 minutes. Cool to 47 o C and aseptically dispense into sterile containers. Optional (not required for use with Solus Listeria test kits) : for visualisation of the aesculin reaction, prior to dispensing into final containers add 2 vials of F.A.C. supplement (SUP004). Appearance: Straw opalescent broth with yellow fluorescence. SOLUS SCIENTIFIC Solus Scientific Solutions Ltd 9 Mansfield Networkcentre, Millennium Business Park, Concorde Way, Mansfield, Nottinghamshire NG19 7JZ Tel +44 (0)1623 429701 Fax +44 (0)1623 620977 Email [email protected] Web www.solusscientific.com Registered Address: East Kilbride Laboratory, Torus Building, Rankine Avenue, East Kilbride, Glasgow, Lanarkshire G75 0QF Company Reg. No. SC361616, Registered in Scotland VAT No 982 2304 24 Certificate Number 11117 ISO 9001

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   Product  Information      Solus  Half  Fraser  Broth  (FBH001,  FBH002,  FBH005)  

         

Formulation                        Grams/litre    

Peptone  mixture             15.0  Yeast  extract                  5.0  Aesculin                  1.0  Disodium  hydrogen  phosphate            9.6  Potassium  dihydrogen  phosphate            1.35  Sodium  chloride                20.0  Lithium  chloride                3.0  Acriflavine                  0.0125  Nalidixic  Acid                  0.01        Directions    Weigh  55  grams  of  powder  and  dispense  into  1  litre  deionised  water.    Allow  to  soak  for  10  minutes,  swirl  to  mix  and  sterilise  at  121oC  for  15  minutes.  Cool  to  47oC  and  aseptically  dispense  into  sterile  containers.  Optional  (not  required  for  use  with  Solus  Listeria  test  kits)  :  for  visualisation  of  the  aesculin  reaction,  prior  to  dispensing  into  final  containers  add  2  vials  of  F.A.C.  supplement  (SUP004).      Appearance:    Straw  opalescent  broth  with  yellow  fluorescence.    

 

SOLUSS C I E N T I F I C

Solus Scientific Solutions Ltd

9 Mansfield Networkcentre, Millennium Business Park, Concorde Way, Mansfield, Nottinghamshire NG19 7JZ Tel +44 (0)1623 429701 Fax +44 (0)1623 620977 Email enquiries@solusscientif ic.com Web www.solusscientif ic.com

Registered Address: East Ki lbride Laboratory, Torus Building, Rankine Avenue, East Ki lbride, Glasgow, Lanarkshire G75 0QF Company Reg. No. SC361616, Registered in Scotland VAT No 982 2304 24Certificate Number 11117

ISO 9001