SMART AUTO STAINER KS-S100Series

http://www.koreastandard.kr

User Operation Manual

SMART AUTO STAINER

KS-Innotech

KS-S100Series

Please avoid wet hands and wear static protective gloves while contacting the device.

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Thank you very much being a precious family of KS Corporation.

This Manual is made by KS Corporation especially for our customers.

Please read it thoroughly before the product is used.

If you have any needs, please contact our factory.

This product is to be used in professional institutions licensed a medical device in China.

In any cases, do not separate this product with wet hands.

It could damage to life and body by an electric shock.

Manufacturer : Koreastandard Co.,Ltd. License No. : Gwangju 12-18

Tel. +82-50-2222-7005 / Fax. +82-61-334-8962

E-mail. Naju factory [email protected] / Head office [email protected]

Features -------------------------------------------------------------------------------------------------------- 2, 3

Package Contents ---------------------------------------------------------------------------------------------3

Equipment layout ----------------------------------------------------------------------------------------------4

Settings and Function -----------------------------------------------------------------------5, 6, 7, 8, 9

SMU(Slide Mount Unit) Mounting & Removal -------------------------------------------10

Reagent Exchange and Prime -------------------------------------------------------------11, 12, 13

Getting Started ------------------------------------------------------------------------------------------------14

Management Tips ------------------------------------------------------------------------------------------15

Checklists before request for troubleshooting -------------------------------------------16

Equioment Specification and Private Reagent Composition -------------------- 18

Regular Inspection lists -------------------------------------------------------------------------------- 17

Stain Process -----------------------------------------------------------------------------------19,20,21,22

Error sign on LC Display board -------------------------------------------------------------------- 23

Simple manual ------------------------------------------------------------------------------------------------24

Option (Multi stainer) setting ----------------------------------------------------------------------- 25

1 to 20 Slides available

Fresh and innovative air-heating system prevents the specimens from overheat. And also you can verify the inside

temperature displayed on the panel in real-time basis.

Total 40 Slides available with option SMU

Our unique SMU design allows to minimize the cross-

contamination dramatically by SMU-cleaning system and

unique SMU-design.

SMU I SMU II(Option)

Automatic clean function is essential to sustain the

trouble free status in nozzle and valve.

Dual-injection type design allows fast injection, and help to

take effects equally on all the slides.

Display real-time temperature Air-heating system

Maximum quantity

Automatic clean

Preventing cross-contamination

Dual-injection type design

page 2

1. Smart stainer 2. Drain hose

5. User manual 6. Power cable

7. Reagent tubes

Visible window allows to see inside while running.Various staining processes are possible with single

equipment by replacing the pump and the piping

system.

3. Reagent holder cable

4. Privatereagent holder

Visible acrylic windowPump valve module

page 3

1. Display screen --------------------------------------- 1

2. Function selection switch ---------------------------------------- 2-1, 2, 3, 4, 5, 6, 7

3. Reagent connector --------------------------------------- 3-1, 2, 3, 4

4. Reagent nozzle --------------------------------------- 4-1 ,2, 3, 4, 5, 6, 7, 8, 9, 10

5. Private reagent holder --------------------------------------- 5-1, 2, 3, 4

6. Reagent sensor cable --------------------------------------- 6

7. Door --------------------------------------- 7-1

8. SMU I, II --------------------------------------- 8-1

9. SMU holder --------------------------------------- 9-1, 2

10. Integrated power switch--------------------------------------- 10

11. Leveling screws --------------------------------------- 11-1, 2, 3, 4

12. D.W connector ---------------------------------------- 12-1, 2, 3, 4, 5

13. Drain hose connector ---------------------------------------- 13

14. Fan ---------------------------------------- 14

15. SMU position sensor 15

16. Temperature sensor 16

----------------------------------------

----------------------------------------

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14

3

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1

1

2

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7

8

1

page 4

When turning "ON" the equipment, start-up screen below is displayed.

State of reagent

Staining method

Function

Current state of the stainer

Remaining process time No. of slides

Setting up slide quantity

Start-up screen

Current internal temperature of the stainer

1. Press 'UP/DOWN' Key to set up the quantity on the

start-up screen.

RESET

LINE

CLEAN

SMU

CLEAN

STAIN

UP

SELECT

DOWN-ESC- -ENTER-

page 5

Setting up waiting time

1. Press 'SELECT' key.

SMU

2. Press 'SELECT' key again when 'MODE' is blinking

If press BASIC, the waiting time will be reset to factory settings. Choose it carefully.

3. Move the cursor to 'SELEC' on 'WAITING' by using

'UP/DOWN' key.

4. Choose the reagent with 'UP/DOWN' key,

and increase/decrease the waiting time with 'UP/DOWN' key.

5. Move to 'STORAGE' or 'ESC' with 'UP/DOWN' key,

and choose what you want with 'SELECT' key.

For save, press 'SELECT' key when the cursor is blinking on 'STORAGE',

and press 'SELECT' key when the cursor is on 'ESC' to go back to previous

screen.

6. Press 'SELECT' key one more time to go back to start-up screen.

RESET

SMU

CLEAN

LINE

CLEAN

STAIN SELECT

UP

DOWN

-ENTER--ESC-

page 6

SMU

2. Press 'SELECT' key again when 'MODE' is blinking

If press BASIC, the injection time will be reset to factory settings. Choose it carefully.

Setting up reagent dose


3. Move the cursor to 'SELEC' on 'INJECTION' by using

'UP/DOWN' key.

4. Choose the reagent with 'UP/DOWN' key, and

increase/decrease the injection time with 'UP/DOWN' key.

5. Move to 'STORAGE' or 'ESC' with 'UP/DOWN' key, and choose what you

want (STORAGE or ESC) with 'SELECT' key. For save, press 'SELECT' key when the

cursor is blinking on 'STORAGE', and press 'SELECT' key when the cursor is

on 'ESC' to go back to previous screen.

6. Press 'SELECT' key one more time to go back to start-up screen.

RESET

SMU

CLEAN

LINE

CLEAN

STAIN

DOWN

SELECT

UP

-ENTER--ESC-

page 7

2. Reagent system (valve, pump and tubing) is automatically filled with D.W. after draining reagent

in the system.

LINE CLEAN is strongly recommended when the equipment is not used, since LINE CLEAN

is closely related to the durability of the equipment.

LINE CLEAN

1. Press 'LINE CLEAN' key on the selection switch.

When D.W. is filled within the reagent system, the alarm message of 'D.W in LINE' is

displayed on the main screen. It prevents the contamination of specimens by D.W. in the

system.

If alarm message is displayed, press 'SELECT' key to go back to main screen and press LINE CLEAN

button.

Reagent is automatically refilled while D.W. is drained from the system.

SMU CLEAN

1. Press 'SMU CLEAN' key on the selection switch.

2. Cleaning solution and D.W. are sequentially injected to SMU to eliminate residual staining reagent

on it.

SMU CLEAN is strongly recommended when the equipment is not used, since SMU CLEAN

is closely related to the durability of the equipment.

RESET

LINE

CLEAN

SMU

CLEAN

STAIN

UP

DOWN

SELECT

RESET

LINE

CLEAN

SMU

CLEAN

STAIN

UP

DOWN

SELECT

-ENTER-

-ENTER-

-ESC-

-ESC-

page 8

1. This key functions to stop equipment upon emergency.

2. Press RESET key, and the slide quantity and current process will return to the initial state except

for a standby time and reagent injection time.

R E S E T

3. When rerun the machine, readjust the slide quantity in advance.

Do not use this key if possible except unavoidable cases (addition of slide, fire due to overheating

of the product, or product failure, etc.).

Excessive operation of all parts may cause a failure. Please be careful when using.

Pressing RESET key when SMU rotates at a high speed may cause an overload to the motor.

RESET

LINE

CLEAN

SMU

CLEAN

STAIN

UP

DOW

N

SELECT

-ENTER--ESC-

page 9

SMU II Attachment

SMU II

Attach SMU II to a combination of SMU I and spacer and then fasten. (Put it into No.1

The SMU must conform to No.1 and No.21 holes to ensure exact mounting.

and No.21 holes.)

Necessarily verify the exact position of the hole before mounting.

* Remove it in reverse order of mounting.

page 10

Reagent Exchange

3. Perform Prime in an auto or manual mode.

4. Perform Staining.

1. Put reagent into a reagent holder in a given order.

the body of the product, and combine them.

Auto prime

2. Open a reagent lid, replace it with a reagent injection lid connected with

1. Press SELECT key in the main button.

2. When MODE flashes, press SELECT key once more.

3. Use UP/DOWN key to move to AUTO of IN.CONFI,

and press SELECT key to run Auto Prime.

RESET

SMU

CLEAN

LINE

CLEAN

STAI

N SELECT

UP

DOWN-ENTER--ESC-

page 11

Manual prime


2. When MODE flashes, press SELECT key once more.

3. Use UP/DOWN key to move to MANU of IN.CONFI, and press SELECT key.

4. Use UP/DOWN key to move to a reagent to be primed, and

press SELECT key. The reagent will be injected while pressing the key.

Pressing 'SELECT' key for too long increases the reagent consumption. Press it for a proper time

to come out the reagent.

RESET

SMU

CLEAN

LINE

CLEAN

STAIN

DOWN

SELECT

UP

* Use UP/DOWN key to set the machine to ESC, and press

SELECT key. The initial screen will be displayed.

-ENTER--ESC-

page 12

Filling of the reagent after line cleaning


2. MODE key flashes.

3. Press LINE CLEAN key.

3. The reagent will be automatically filled for staining.

RESET

SMU

CLEAN

LINE

CLEAN

STAIN

DOWN

SELECT

UP

-ENTER--ESC-

RESET

SMU

CLEAN

LINE

CLEAN

STAI

N

DOWN

SELECT

UP

-ENTER--ESC-

page 13

* See the name of each part (P.4).

2. The message as shown will be displayed

1. Turn on the power switch (10) on the rear side of the product.

on the screen display (1).

(Preheating is unnecessary. The machine is designed to prevent overheating.)

3. Check the quantity of staining slide, set the desired quantity using UP/DOWN key,

and put the slide in sequence (from No.1) on SMU 8-1.

- The standard time is set in a factory prior to shipment. You don't have to set it separately.

- Staining does not start before the door closes.

4. Close Door (7), press STAIN key, and a fully automated standard staining will start.

5. When completion, a buzzer sound occurs. The screen display returns to the initial

standby state. Then, open the inner door and take out a stained slide.

Adjust the balance of the auto stainer before use.

page 14

LINE CLEAN

SMU CLEAN

Inner Door

initial state.

After daily or once use, press SMU CLEAN key to prevent SMU from contamination. This can prevent the

Do not touch the machine with wet hands. Wear gloves to prevent electric shock.

Before powering off after daily use, surely press LINE CLEAN key to exhaust reagent residue in the pump and

pipe and nozzle so that the machine cannot be oxidized, maintaining the high-quality staining result like the

(Soak it into tap water before leaving the office, and attach it to the machine after going to the office.)

* After daily use, separate SMU from the machine, and soak it into tap water about for 8 hours.

You can use it as comfortably as new equipment for a long term.

deviation of slides during staining and reduce the relative risk of cross-contamination.

by special coating. But, it is difficult to protect the machine from the phenomenon completely. If the machine is

Body Management

contaminated by reagent, wipe it with a clean swab or machine-dedicated cleaning reagent to prevent it from

The machine is always exposed to contamination and corrosion due to reagent. To prevent these, it is protected

being contaminated to the maximum.

reagent during use, its inner clarity is reduced. Thus, immediately wipe it with a clean swab or cleaning reagent.

The inner door is made from semi-transparent acrylic material for user's convenience. If it is contaminated by

page 15

No. Symptom Checklist Troubleshooting

1 1. Is a power cord connected firmly? 1. Connect a power cord with dry hands.

2 2. Did you check fuse? 2. Power off and insert a spare fuse.

3 3. Have you used other equipment power cord 3. Connect a normal power cord checked with dry

4 to check whether the machine is powered on? hands, or replace a defective outlet with a spare part.

51. Is a dedicated reagent tube connected

normally?

1. Connect a dedicated reagent tube

and connector.

62. Is more than 2/5 of a water bucket for

washing filled with water?2. Refill washing water.

73. Have you used the machine immediately

after line clean?3. Set auto-filling of reagent.

8 4. Is the power disconnected during use?4. Press STAIN key once again to start

re-staining.

9 1. Is part of a drain hose pinched or folded? 1. Loosen the pinched or folded part.

102. Does a slide broke or include foreign

substances?

2. Remove the broken slide and foreign

substances.

113. Is a drain hose put into a waste liquid

bucket?

3. Put the drain hose on the higher place

than the waste liquid level

121. Is more than 2/5 of a dedicated cleaning

bucket filled with cleaner?

1. Fill the bucket with a dedicated

cleaning reagent.

132. Does the machine operate when pressing

CLEAN key?

2. Power off and press the key more than

20 times.

14 1. Has liquid splashed or dripped? Never disassemble or touch the machine

152. Is there any leakage from the bottom of

the machine?

randomly. Otherwise, it may cause electric

shock. Pease ask specialists.

16 1. Is the pump normal?1. Replace the pump with a spare part

referring to the service manual.

17 Abnormal 2. Is the valve normal?2. Replace the valve with a spare part

referring to the service manual.

18 reagent3. Is the power of the PCB pump and valve line

power normal?3. Replace the main PCB with a spare part.

19 injection4. Is the connector inserted into the PCB

connected normally?4. Connect the disconnected connector again.

20 5. Check whether the reagent nozzle clogs. 5. Drill clogging using a specialized tool.

20 1. Check a LCD socket.1. Separate it from the machine, apply anti-

corrosion lubricant, and insert it into the

machine.

21 2. Check whether the CPU is defective. 2. Replace the CPU with a spar part.

22 Power off and press the key more than 20 times.1. Separate the relevant part, apply lubricant,

and insert it into the machine.23 Check whether the PCB is defective. 2. Replace it with a spar part.

24 Other

Abnormal

staining

Abnormal

power

Abnormal

clean

function

Abnormal

drain of

waste

liquid

Equipment

leakage

If the symptom is not improved after troubleshooting, contact your purchase agent.

The following is checklists for specialists (Never touch the followings except for designated specialists. Otherwise,

it may cause electric shock or physical injury.)

Abnormal

contact of

switches

Letters on

the screen

are

broken.

Contact the head office or refer to the service manual.

page 16

●Exchange ○Inspection

1Check whether there is any moisture or

water around the machine. ○

2Check whether the floor of

the machine leaks. ○

3Check whether the power

switch is on or off. ○

4 Check the screen display. ○

5Check whether function keys operate

normally. ○

6Check whether the fan operates

normally. ○

7Replace the fan filter (depending upon

the quantity consumed). ●

8Check whether the reagent sensor

operates normally. ○

9 Check the remaining reagent. ○

10Replace the reagent (depending upon

the quantity consumed). ● ○ ○ ○ ●

11Replace the cleaning reagent (depending

upon the quantity consumed). ● ○ ○ ○ ●

12Replace the distilled water (depending

upon the quantity consumed). ● ○ ● ○ ○

13 Clean the nozzle. ○ ○ ○ ○ ○

14 Clean the tube. ○ ○ ○ ○ ○

15 Clean the valve. ○ ○ ○ ○ ○

16 Clean the pump. ○ ○ ○ ○ ○

17 Clean the SMU. ○ ○ ○ ○ ○

18 Clean the chamber. ○ ○ ○ ○ ○

19 Clean the door. ○ ○ ○ ○ ○

20 Clean the body of the machine. ○ ○ ○ ○ ○

21Check the motor performance

(by engineers). ○

YearlyDaily Weekly Monthly Quarterly Inspection Items

Inspection CycleSequence

* If possible, please clean the stainer before holiday. It is effective to set the machine before staining.

page 17

Function

Slide Mount Unit capacity 1 to 20 Slides - Total 40 Slides with option SMU

Pre-defined temperatire Air-heating system with real-time temperature monitoring - (60℃-70℃)

Staining cycle Reagent injection (Fixing -Primary Stain -Decolorizing -Background stain -Dry)

Rotational speed [rpm] 3rpm ~ 200rpm ( Option : 1,500 ~ 2,000rpm)

Basic functions Stain, Automatic Cleaning (SMU, Tube, Pump, Valve), Dry, Slide quantity setting,

Reagent injection (Auto, Manual), injection volume setting, Stop

Electrical Requirement 230VAC, 50Hz / Run 50W, (Heating:950W)

Dimensions / Weight W*D*H=507mm*554mm*274mm / approx. 20kg

Reagent Consumption Approximately 0.6ml ~ 2ml / Slides

1 Cycke staub time 5min ~15min / vary with wating time and slide quantity

SMU (Slide Mount Sytem) SMU I, II (option)

AFB

[Ziehl-Neelsen/Kinyoun] 1. Carbol Fuchsin 2. Decolorizer 3. Methylene blue

AFB [Flourochrome] 1, Auramine rhodamine 2. Decolorizer 3. Pot.permanganate

GRAM 1. Crystal violet 2. Gram iodine 3. Acetone alcohol 4. Safranin

Hematolgy

[Wright, Wright/Giemsa]

Pap [Papanicolaou] 1. Hematoxyline 2. OG-6 3. EA-50

DNA 1. Phosphate buffer 2. Modified Giemsa

Specification

1. Wright 2. Phosphate Buffer

Some content or design of the equipment is subject to change without prior notice for

performance

page 18

STAIN : Fluorochrome

*HEAT:22`C *R.GENT:on

*State:1R Injection *Menu

*R/T:17m00 *No.:20ea



*State:5R Washing *Menu

*R/T:17m00 *No.:20ea

STAIN :

Fluorochrome






*R/T:17m00 *No.:20ea



*State:Drying.......... *Menu

*R/T:17m00 *No.:20ea

Fluorochrome




*R/T:17m00 *No.:20ea




*R/T:17m00 *No.:20ea




*R/T:17m00 *No.:20ea




*R/T:17m00 *No.:20ea




*R/T:17m00 *No.:20ea

page 19

STAIN : Ziehl-Neelsen



*R/T:17m00 *No.:20ea




*R/T:17m00 *No.:20ea




*R/T:17m00 *No.:20ea



*State:Drying...........*Menu

*R/T:17m00 *No.:20ea

Ziehl-Neelsen




*R/T:17m00 *No.:20ea




*R/T:17m00 *No.:20ea




*R/T:17m00 *No.:20ea




*R/T:17m00 *No.:20ea




*R/T:17m00 *No.:20ea



*State:Heating....... *Menu

*R/T:17m00 *No.:20ea



*State:Heating....... *Menu

*R/T:17m00 *No.:20ea

page 20

STAIN : Gram



*R/T:17m00 *No.:20ea

STAIN : Gram



*R/T:17m00 *No.:20ea

STAIN : Gram



*R/T:17m00 *No.:20ea

STAIN : Gram



*R/T:17m00 *No.:20ea

STAIN : Gram


*State:Drying........... *Menu

*R/T:17m00 *No.:20ea

Gram

STAIN : Gram



*R/T:17m00 *No.:20ea

STAIN : Gram


*State:5R Washing *Mode

*R/T:17m00 *No.:20ea

STAIN : Gram



*R/T:17m00 *No.:20ea

STAIN : Gram



*R/T:17m00 *No.:20ea

STAIN : Gram



*R/T:17m00 *No.:20ea

page 21

STAIN : Hematology


*State:1R Injection *Mode

*R/T:17m00 *No.:20ea

STAIN : Hematology


*State:Mixing........ *Menu

*R/T:17m00 *No.:20ea

STAIN : Hematology



*R/T:17m00 *No.:20ea

STAIN :

Hematology


*State:Drying........... *Menu

Hematology

STAIN : Hematology



*R/T:17m00 *No.:20ea

STAIN : Hematology



*R/T:17m00 *No.:20ea

page 22

Display : Reagent shortage & of Reagent holder error

Error sign : "1R,2R,3R,4R" displayed when the reagent shortage

& Reagent holder error

Display : Temperature sensor error

Error sign : "High" displayed when the Temperature sensor

error & reagent flowed on the Sensor

Display : Motor Error

Error sign : "Motor Error" displayed when the magnet error on SMU

& Center Sensor error in front of Chamber

Display : Reagent shortage after Line Clean

Error sign : "No. R_Gen. in Lines" displayed when Reagent

shortage & Reagent supplement after Line Clean

Display : Door Cover Open

Error sign : "Cover opened" displayed when Door Sensor

error & Door Cover Opened


*HEAT:22`C *R.GENT:1R

*State: Wait *Mode

*R/T:17m00 *No.:20ea


*HEAT:High *R.GENT:on

*State: Wait *Menu

*R/T:17m00 *No.:20ea

--- Motor Error ---

Esc

-- No. R_Gen. in Lines --

Cover Open

----- Warning -----

----- Cover Opend -----

Esc

page 23

State of reagent

Function

No. of Slides

Current state of the stainer

1. UP/Down Key : Set-up for Q'ty of Slides.

2. Stain Key : Setting for stainer's performance

3. Reset Key : Stop the process immediately and reset in emergencies.

4. Line Clean Key : Auto clean setting for pumps, valves, tubes and nozzles.

5. SMU Clean Key : Auto clean for SMU(Slide Mount Unit).

6. Select Key : Select waiting-time, injection time and options for the reagents.

Staining method

Current internal temperature of the stainer

Remaining process time

Function Key

Start-up screen

When turning "ON" the equipment, start-up screen below is displayed.

RESET

LINE

CLEAN

SMU

CLEAN

STAIN

UP

SELECT

DOWN

-ESC--ENTER-

1

6

2

4

3

5

page 24


SMU

2. Choose the Model using UP/Down key while 'MODE' flickers.

You can store Model using 'SELECT Key.

Option ( Multi Stainer ) Model Setting

3. Once Ziehi-Neelsen Model setting, press 'Select Key.

4. Once Flourochrome Model setting, press 'Select Key.

5

5. Once Gram Model setting, press 'Select Key.

Once 'SELECT' Key setting, please be careful not to change the model.

STAIN : Ziehi-Neelsen


*State:Wait *Mode

*R/T:17m00 *No.:20ea

RESET

SMU

CLEAN

LINE

CLEAN

STAIN SELECT

UP

DOWN-ENTER--ESC-

STAIN : Flourochrome


*State:Wait *Mode

*R/T:17m00 *No.:20ea

STAIN : Gram


*State:Wait *Mode

*R/T:17m00 *No.:20ea

page 25

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SMART AUTO STAINER KS-S100Series