Hyphenated Techniques

Smah Abdelhamid

Its an analytical techniques that couples

chromatographic and spectral methods to exploit the advantages of each Chromatography: identify chemicals purely or

nearly purely in complex mixtures. Spectroscopy: yields qualitative information

about pure component. Introduce to the scientific community in 1980,

by Dr. Tomas Hirschfeld an analytical chemist

What?

Highly selective Highly sensitive Use in many Applications

purifications Polymerization studies Natural products Pharmaceutical Many more….

Why?

Very sensitive and selective Most common Sample is injected into injection port of GC,

than its vaporized, separated in the GC column, than Analyzed by MS detector, and recorded

Provides structural information Volatile compounds Polar compounds

GC-MS

GC-MS

Applications Environomental

monitoring and cleanup organic pollutant

Criminal forensics Drug detections Fires investigations Explosive investigations

Sports Medicine

Double three way diverter in line with an auto

sampler, and LC system, and the MS Second most common Poor structure determination

LC-MS-MS Increase fragmentation

LC-UV-MS Biologhical screening for natural products

LC-MS

LC-MS

Applications

Pharmacokinetics Bioanalysis

Proteomics Metabolomics Drug development

Least sensitive High cost Provide best structural information Analysis of natural products Analysis of drug related metabolites in bio-fluids

and biomolecules in general analysis of organic molecules Drug impurities Reaction mixtures Drug degradation products

HPLC-NMR

HPLC-NMR

Autosampler, LC pump, Column, a non-NMR detector

Flow is guided in the LC-NMR interface

Than its guide it to the Flow-cell NMR probe Head

Following that the flow is routed to a fraction collector for recovery or further invegistation.

CE: Capillary Electrophoresis

Analysis is driven by an electric filed in narrow tubes

Results in fast separation of compounds (almost all compounds can be separated through this methods)

Analysis of purities Assays Analysis of trace level determination

CE-MS

CE-MS

CE-UV GC-FTIR

Identifying isomers Ephendrine-type alkaloid found in dietrary

supplements containing the chinese herb ma huang

HPLC-ESI-MS HPLC-PDA

Analysis of coumarins

Other Hyphenated Techniques

Spectra

GC-MS LC-MS

Spectra

HPLC-NMR CE-MS

Conclusion

Since the introduction of hyphenated techniques in the 1980s they under went great evolution due to technology, which has expand its use and applications.

1. Patel, K., Patel, J., Patel, M., Rajput, G., & Patel, H.

(2010). Introduction to hyphenated techniques and their applications in pharmacy. Pharmaceutical Methods, 2(1), 2. doi:10.4103/2229-4708.72222

2. The Hy-phen-ated MethodsTomas HirschfeldAnalytical Chemistry 1980 52 (2), 297A-312A

3. http://www.sigmaaldrich.com/analytical-chromatography/analytical-standards/application-area-technique/standards-for-iso-188572.html (images)

4. http://www.nature.com/nchembio/journal/v2/n8/fig_tab/nchembio803_F4.html

Reference