Slides for Histo Practical Exam

List of Slides for The Practical Exam

1). Cardiac Muscle (H & E)

Slide #5:Heart Wall; Cross Section

Visible at the top of the photo are the Perkingy Fibers (large, “lumpy-looking” fibers), responsible for causing the contractions of the heart.

Note: The nuclei of heart muscle cells is at their periphery as wellH & E Staining(Low Magnification)

Slide #5:Heart Wall; Cross Section

Visible at the top of the photo are the Perkingy Fibers (large, “lumpy-looking” fibers), responsible for causing the contractions of the heart.

Beneath the Perkingy Fibers are visible the Cardiac Muscle Cells, and at the periphery of these cells, stained a very definitive purple are the nuclei.

H & E Staining(Medium Magnification)

2). Blood Smear (May-Grunwald-Giemsa Staining)

Blood SmearMay-Grünwald-Giemsa Staining

Neutrophil, a.k.a Polymorphonuclear Leukocytes (with 4 nuclei; depicting its “old” status). Pink Cytoplasm.

Surrounding the Neutrophil, many Red Blood Cells (anucleated, of course)

(High Magnification)

Blood SmearMay-Grünwald-Giemsa Staining

Neutrophil, a.k.a Polymorphonuclear Leukocytes (with 2 nuclei; depicting its “young” status, as compared to the previous picture). Pink Cytoplasm.


TV Screen:Blood Smear

Eosinophil. The DEEPLY/INTENSELY RED STAINED granulocytes cover most of the cytoplasm and make this cell easily recognizable.

The Nucleus also has only 2 lobes.

May-Grünwald-Giemsa Staining(High Magnification)


Eosinophil. The DEEPLY/INTENSELY RED STAINED granulocytes cover most of the cytoplasm and make this cell easily recognizable.

The Nucleus also has only 2 lobesMay-Grünwald-Giemsa Staining(High Magnification)


Basophil. Slight “pinky” border with dark blue granules COVERING THE ENTIRE CYTOPLASM. The cell is so internally granular that the nucleus is invisible.

It is easy to differentiate between Basophils and other white blood cells due to its granularity.



Monocyte. Monocytes are MUCH LARGER THAN Neutrophils, and their kidney-bean shaped nuclei make them easily distinguishable



Lymphocyte. Very small sized. Dark coloured cytoplasm with a small rim of pink-coloured cytoplasm. These cells are the B cell and the T cells.



Platelets. Seen in this photograph in the left part of the TV screen. Responsible for blood-clotting.

May-Grünwald-Giemsa Staining



Eosinophil (bottom & towards the left) and Neutrophil (atop the Eosinophil). Easily distinguishable due to the Eosinophils intense colour in the cytoplasm, and due to the Neutrophils multiple nuclei and pink cytoplasm.


3). Lymph Node (H & E)

Slide #1:Lymph Node

Visible in this photograph are the lymphatic outer sinuses (spaces within lymph nodes), as well as within the medulla, the medullary sinuses are visible

The dense purple tissue is the cortexThe majority of what is visible in this photo is the dark purple cortex

The resident cells (T Cells) are purple within the lymph nodules The pink stained areas are merely connective tissue

H & E Staining(Low Magnification)

Slide #1:Lymph Node

In this photograph, prominently visible, are the cortex and lymphatic zones.H & E Staining(High Magnification)

4). Palatine Tonsil (H & E)

Slide #3:Palatine Tonsil

Very visible in this photograph are the dark purple stained lymphocytes, and the “round-shaped” structures are the arteries with their very thick walls. At the lower left 1/3 of the photograph are visible the veins, which appear as elongated structures.

H & E Staining(High Magnification)

5). Lingual Tonsil (H & E)

Slide #6:

Lingual TonsilVisible in this photograph are: Mucous glands (left of image), and directly below them is visible a very large vein (orangy-red colour). As well, dark-purple stained region is the germinal center, within its periphery which are visible several arteries (to its left). Furthermore, the elongated-appearing structures which have the reddish staining are the venuoles.


6). Spleen (H & E)

Slide #2:Spleen

The Capsule, trabeculae, trabecular veins and arteries, white pulp (PALS, lymphatic nodules), red pulp (splenic cords and sinuses), and pulp veins are visible.

--The pulp veins are visible here as the most easily visible, reddish circular objects within the white pulp--The capsule is visible encircling the periphery--The sinusoids are MUCH smaller than the pulp veins, yet also have the same colour as the veins. In this picture, the sinusoids are dispersed throughout the image, with several concentrated near the capsule


Slide #2:Spleen

At the top of the image is visible the capsule, which appears as a band at the top of the photo.As well, prominently visible in this photo is the “pulp vein” in the center of the image, with its deep red colour. As well, several dark purple lymphatic nodules are visible.

NOTE: Lymphatic nodules ONLY appear during an immune response!


Slide #2:

SpleenProminently visible in this photo is the “pulp vein” in the center of the image, with its deep red colour. As well, several dark purple lymphatic nodules are visible.

NOTE: Lymphatic nodules ONLY appear during an immune response!


Slide #2:Spleen

The most prominent feature of this image is the Trabeculae, which can be seen here branching and stemming from the right of the image. Within its center can be seen the trabecular artery.


7). Thymus-Juvenile (H & E)

Slide #4:Thymus-Juvenile

The light pink stained regions are the Medulla (Mature Thymocytes present); –ve Selection hereThe dark purple stained regions are the Cortex (Immature Thymocytes present); +ve Selection here

The lymphoid nodules are easily identifiable in the Cortex as several dark purple regions

H & E Staining (Low Magnification)

TV Screen:Thymus-Juvenile

In this middle of this photo are visible a couple of easily identifiable Hassal’s Corpuscles (“Hassal Bodies”)

---Hassal Bodies, ONLY present in the Medulla Surrounding the Hassal’s Corpuscles, the Cortex

H & E Staining (Low Magnification)

Slide #4:Thymus-Juvenile

In this picture is an easily identifiable Hassal’s Corpuscles (Hassal Body)---Hassal Bodies, ONLY present in the Medulla

---Reticular cells can also be seen in the vicinity of the interior of the Hassal Body

H & E Staining (High Magnification)

8). Thymus in Involution (H & E)

Slide #5:Thymus in involution

Involution: A progressive decline or degeneration of normal physiological functioning occurring as a result of the aging process

The white globular cells are adipose tissueThe dark concentric regions within the cortex are hyalized cells

Note: The cortex is the dark purple stained regions (right of photo) containing the immature thymocytes Note: The medullary region contains the mature thymocytes (center of photo), with the hyalized cells within it


Slide #5:Thymus in involutionH & E Staining(High Magnification)

9). Thyroid Gland with Parathyroids (H & E)

Slide #4:Thyroid and Parathyroid Gland

At the centre of this image is a typical colloid cell, and surrounding it in a disk are the Thyroid Follicular Cells. Around the Thyroid Follicular Cells lies the Basal Lamina.

Although H & E DOESN’T stain for it and they are not readily visible in this image, Parafollicular cells (“C” cells; calcitonin producing) lie around the Thyroid Follicular Cells.


Slide #4:Thyroid and Parathyroid Gland

Visible in this photo are the paraffin-embedded Adipose TissueH & E Staining(High Magnification)

10). Adrenal Gland (H & E)

Slide #2:Adrenal Gland

In this image are visible large PURPLE stained nuclei (i.e., similar staining pattern to Lymphocyte nuclei because the nucleus to cell size ratio is high in both these types of cells).



Visible from the bottom left of the image to the top right of the image are the 3 regions of the gland:1). Zona Glomerulosa 2). Zona Fasciculata

3). Zona ReticularisNote: There is also adrenal medulla as well.



From Right to Left in this image are:The Capsule and 1). Zona Glomerulosa and 2). Zona Fasciculata


11). Supraoptic and paraventricular nuclei of the hypothalamus (Gomori Staining) NOTE: Supraoptic tested ONLY!

Slide #P:Supraoptic and paraventricular nuclei of the hypothalamus

Visible in this photo: Supraoptic NucleiGomori Staining(High Magnification)

Slide #P:Supraoptic and paraventricular nuclei of the hypothalamus

Visible in this photo: Paraventricular Nuclei (look “above and below” to the left of the raphe/slit in the middle of the image. The cluster of nuclei there are the paraventricular nuclei)

Gomori Staining(High Magnification)

12). Hypophysis (Mallory Staining)

Slide #1:--Microscopic View

HypophysisVisible in this photo: 1). Acidophiles (Reddish-orange stained), 2). Basophiles (Blue stained), and

3). Connective tissue (stained blue) Visible as well: 4). “Chromophobes”: appear as empty “white” spaces

(Chrom: colour phobes: “haters”) These cells secrete: Vasopressin and OxytocinMallory Staining(Low Magnification)

13). Nonhairy skin-longitudinal section (H & E)

Slide #2:Nonhairy Skin-longitudinal section

Visible in this photo are several layers of the skin (from top left to bottom right): The 1). stratum corneum (the top layer), 2). stratum lucidum (red band), 3). stratum granulosum (dark purple band beneath the stratum lucidum), 4). stratum spinosum, and 5). stratum basale. The 6). connective tissue is beneath the stratum basale. The visible “grooves” between the stratum basale and the connective tissue provide for a more secure lock-and-key mechanism between the two layers.


14). Hairy Skin-longitudinal section (H & E)

Slide #1:Hairy Skin-longitudinal section

Visible in this photo are (from follicle out): The cortex, melanocytes (black specks at bulb of hair) with dermal papilla just inferior to them, the internal root sheath and the external root sheath, and the outside layer, the glossy membrane.


Slide #1: TV Screen:Hairy Skin-longitudinal section

Prominently visible in the central part of this photo, a sebaceous gland.H & E Staining(High Magnification)


Visible in this photo are (from follicle out): The cortex, melanocytes (black specks at bulb of hair) with dermal papilla just inferior to them, the internal root sheath and the external root sheath, and the outside layer, the glossy membrane.



Visible in the centre of this photo as a coiled structure with black-“specks”, is the sweat gland H & E Staining


15). Hairy Skin-cross section (H & E)

Slide #2:Hairy Skin-cross section

Seen in the centre of the photo are (from medulla outwards): The medulla, cortex, cuticle, internal root sheath, and external root sheath.


16). Lip (H & E)

Slide #1:Lip

Visible in this photo are: The Vermillion Zone (far right, subsurface layer pink-coloured), sebaceous gland, muscle tissue, adipose tissue, and an artery.


17). Tongue-filiform and fungiform papillae (H & E) Note: Fungiform NOT tested

Taste Buds

Foliate PapillaeNote: The Tall structure of the Papillae

Fungiform PapillaeNote: Mushroom Appearance

18). Tongue-filiform and foliate papillae and taste buds (H & E)

Filiform PapillaeNote: These taste buds are VERY shallow and easy to recognize

19). Developing tooth (Mallory staining)

Slide #2:Developing Tooth

Visible in this photo are (from far right to left): Pulp, Odontoblasts (secrete Dentin), Predentin, Dentin (with the thick, rich red canaliculae), Enamel, and to the far left, Ameloblasts=adamentoblasts (secrete enamel).


20). Adult tooth (Mallory Staining)

Slide #Z:Adult Tooth-Decalcified

Decalcification has dissolved the enamel of this toothMallory Staining(Low Magnification)

21). Parotid Gland (H & E)

Parotid Gland(Low Magnification)

Parotid GlandNote: Invaginations of the epithelium!!!

Low Magnification

Slide #15:Parotid Gland

The duct is prominently visible in the centre of this photoH & E Staining(High Magnification)

22). Sublingual gland (H & E)

Sublingual glandNote: Many mucous tubules; easy to distinguish between sublingual and submandibular

23). Submandibular Gland (H & E)

Slide #17:Submandibular Gland

Ducts are visible at the centre of this photoNote: Presence of mucous tubules and prevalence of DARK serous elements


Submandibular GlandDucts are visible at the centre of this photo

A great MIXTURE of MUCOUS TUBULES and STRIATED DUCTSNOTE: Appears MUCH DARKER than sublingual gland as well!

H & E Staining

24). Esophagus (H & E)

Slide #6: Esophagus

Visible at the the far right of this picture is the Stratified Squamous EpitheliumH & E Staining(Low Magnification)

Slide #6: Esophagus

Visible centrally located in this picture is the smooth musclesH & E Staining(Medium Magnification)

Slide #6: Esophagus

Visible in the centre of this picture is an esophageal glandH & E Staining(Medium Magnification)

Slide #6: Esophagus

Visible in this image are skeletal musclesH & E Staining(Medium Magnification)

25). Gastric fundus-staining for parietal cells (Kongo Red)

Slide #7: Gastric Fundus

This slide has been stained for parietal cellsKongo Red Staining(Low Magnification)

Slide #7: Gastric Fundus

This slide has been stained for “Parietal Cells”o The orange cells located peripheral to the main cells are the “Parietal Cells”

The Parietal Cells are responsible for producing HCL The cells which the Parietal cells are surrounding are the “Chief Cells”

The Chief Cells are responsible for producing PepsinogenKongo Red Staining(Low Magnification)

26). Gastric pylorus (H & E)

Slide #8: Gastric Pylorus

Visible in this slide are (from far left to right): Muscularis Mucosae, the mucous secreting cells located adjacent to the Muscularis Mucosae, and cells of the Gastric Pits (dark purple cells which also produce mucous)

Note: The nerves in the Gastric Plexus. These are nerves of the Auerbach’s PlexusH & E Staining(Low Magnification)

Slide #8: Gastric Pylorus

Visible in this slide is a Nerve passing through the gastric pylorus

Note: The nerves in the Gastric Plexus. These are nerves of the Auerbach’s PlexusH & E Staining(Medium Magnification)

27). Small intestine-duodenum (H & E)

Slide #9: Small Intestine-duodenum

Key Point for Duodenum: Has the LONGEST villi of the 3 regions of the small intestine! Visible in this photo are (left to right): villi, the submucosal region with Brunner’s Glands and the Meissner Plexus, and to the far right, the muscle of the duodenum

Although not visible, the duodenum also has Peyer’s Patches (Lymphoid nodules)H & E Staining(Low Magnification)

28). Small intestine-jejunum (H & E)

Slide #10:

Small Intestine-jejunumKey Point for Jejunum: Has MEDIUM SIZED villi of the three regions, but not as long as

duodenum! Visible in this photo are (left to right): the Meissner Plexus and connective tissue, BUT NO Brunner’s Glands present in Jejunum (contrast with Duodenum), and to the far right, the villi.

Note: The layering of the duodenum, jejunum, and ileum are all the same, except that in the jejunum, there are NO Brunner’s Glands and NO Peyer’s Patches in jejunum, as opposed to the duodenum which DOES possess them.


29). Small intestine-ileum (H & E)

Small Intestine-ileumKey Point for Ilium: Has SHORTEST villi of the three regions!

Visible in this photo are (top to bottom): the SHORT VILLI, and Peyer’s Patches.Note: The layering of the duodenum, jejunum, and ileum are all the same. The differences are that the ileum has the SHORTEST VILLI and DOES HAVE PEYER’S PATCHES.

H & E Staining

30). Large intestine-colon (H & E)

Slide #11: Large Intestine-colonH & E Staining(Low Magnification)

31). Large intestine-appendix (H & E)

Slide #12: Large Intestine-AppendixH & E Staining(High Magnification)

Large Intestine-AppendixNote: The circular structure of the specimen, as well as the LARGE collection of lymphoid nodules in the appendix


32). Human liver (H & E)

Slide #1: Human Liver

Centrally located in this photo is visible the Portal Space, with hepatocytes surrounding it

At the top of the Portal Space is visible an artery (with its thick tunica media), and at the centre of the portal space is visible a massive vein. At base of Portal Space is visible a Bile Duct (with blue stained central region). Directly above the bile duct is an artery, and to the right of the artery and below the massive vein is a Lymph Vessel

Liver also has Ito Cells: Control Vasoconstriction.H & E Staining(High Magnification)

33). Swine liver (H & E)

Slide #2: Swine Liver

Visible in this photo: Top of picture is a Lymph VesselBottom left of picture is the Portal SpaceThe interlying space between the two has many hepatocytes in it.



Visible in this picture is a Liver SinusoidThe purple stained regions are the hepatocytes, and within the liver sinusoids are visible erythrocytes which are red coloured.



Visible in this picture is a Liver Sinusoid

The purple stained regions are the hepatocytes, and within the liver sinusoids are visible erythrocytes which are red coloured.


34). Human liver (Mallory staining)

Slide #2A: Human Liver

Visible in the centre of this photograph is the liver sinusoidMallory Staining(Medium Magnification)

Slide #2A: Human Liver

At the centre of this photo is the Central Vein. Surrounding the central vein are hepatocytes, which are all pointed towards the central vein concentrically!

Mallory Staining(Medium Magnification)

Slide #2A: TV presentationHuman Liver

Visible are the Portal space (within which are erythrocytes, the artery at base of it)

To the right of portal space are visible Ito Cells (brown stained region)Mallory Staining(Medium Magnification)

35). Gall bladder (H & E)

Slide #10: Gall Bladder

Visible in this photograph are columnar epithelium (top layer), and beneath the columnar epithelium is a smooth muscle layer. Directly beneath this layer is the serosa.

Note for the lab exam: The columnar epithelium folds in ALL DIRECTIONS. Also, there are NO GLANDS in the mucosal layer.


36). Pancreas (H & E)

Slide #3: Pancreas

Centrally visible in this photograph is an “Islet of Langerhans”--Visible surrounding the Islet of Langerhans are the “Serous acinus,” which are exocrine cells


37). Pancreas (Brachet’s staining)

Brachet’s staining stains for RNA, and visible under this staining in this slide are RIBOPROTEINS

38). Trachea-cross section (H & E)

Slide #1:Trachea

Visible in this photo are the pseudostratified epitheliumNote for the lab exam: LOTS OF HYALINE CARTILLAGE visible under the epithelium

H & E and PAS Staining(High Magnification)

Slide #1:Trachea

Visible in this photo are: Veins (with dark “blotches” of endothelial cells surrounding them), connective tissue, as well as the pseudostratified epithelium.

H & E and PAS Staining(Medium Magnification)

39). Lung Parenchyma (H & E)

Slide #2:Lung Parenchyma

Visible in this photo are: Bronchi, Bronchioles, and Alveoli, which are part of the Conducting System of the Respiratory Tract. The terminal portion only has Single Alveoli, as opposed to the Respiratory Portion which has several Alveoli. As well, BRONCHIOLES contain NO CARTILLAGE, as opposed to BRONCHI which CONTAIN CARTILLAGE.



Visible in this photo are: Bronchi, Bronchioles, and Alveoli, which are part of the Conducting System of the Respiratory Tract. The terminal portion only has Single Alveoli, as opposed to the Respiratory Portion which has several Alveoli. As well, BRONCHIOLES contain NO CARTILLAGE, as opposed to BRONCHI which CONTAIN CARTILLAGE.



Visible in this photo are: Vein and Alveoli.



Visible in this photo are: Bronchioles.H & E Staining(Low Magnification)


Visible in this photo are: Bronchioles. BRONCHIOLES, as visible here, contain NO CARTILLAGE, as opposed to BRONCHI which CONTAIN CARTILLAGE.



Visible in this photo are: Bronchi, and clearly visible CHONDROCYTES (forming CARTILLAGE). As well, BRONCHIOLES contain NO CARTILLAGE, as opposed to BRONCHI which are clearly visible here and which DO CONTAIN CARTILLAGE.


40). Human kidney (H & E)

Slide #1: Human Kidney

Centrally located in this photo is the Renal Corpuscle and visible is the Bowman’s Capsule; Within the Bowman’s Capsule is the podocyte

To the left side of the podocyte is the Distal Tubule (w/only a fragment of the Afferent Arteriole visible) To the right side of the podocyte is the Proximal Convoluted Tubule

Surrounding the podocyte is the Macula DensaH & E Staining(Medium Magnification)

Slide #1:

Human KidneyCentrally located in this photo is visible the Bowman’s Capsule;

The pointer is pointing to the Distal Tubule within the PodocyteH & E Staining(Medium Magnification)

41). Kidney (injection specimen to demonstrate the vascular bed)


Injection specimen to demonstrate the vascular bed This dye is a special type of liquid dye stain which forms into a gel

Two structures can be seen: 1). Very large vein dyed blue 2). A renal corpuscle within which is visible the

Bowman’s capsule and Distal TubuleH & E Staining(Low Magnification)


Injection specimen to demonstrate the vascular bedVisible in the centre of this image is the Glomerulosa


42). Ureter (H & E)

Slide #4: Human Ureter-transverse section

Visible in this image is transitional epithelium


Slide #4: Human Ureter-transverse section

Visible in this image is transitional epitheliumH & E Staining(Medium Magnification)

43). Urinary bladder (H & E)

Slide #6:

Human Urinary Bladder-cross sectionVisible in this image are the smooth muscles in the urinary bladder


Slide #6: Human Urinary Bladder-cross section

Visible in this image are from (right to left): Transitional epithelium with Lamina Propria beneath it

Note: Distinguishing characteristic is the smooth muscle layer & transitional epitheliumKEY POINT: VACUOLES AROUND THE NUCLEUS!!!


Slide #6: Human Urinary Bladder-cross section

Visible in this image are Nerve Bundles in the urinary bladderH & E Staining(Low Magnification)

44). Testis (H & E)

Slide #1: Human TestisH & E Staining(High Magnification)

Slide #1: Human Testis

Visible in this picture are: Border of seminiferous tubules, Fibroblasts within the border of seminiferous tubules, Sertoli Cells (not round), and Gametogenic cells.


45). Epididymis and ductuli efferentes (H & E)

Slide #2: Human Epididymis

Visible in this picture is the Pseudostratified Epithelium of the Epididymis These are the Ductuli Efferentes (No “cilia”), i.e., the head


46). Spermatic cord with ductus deferens (H & E)

Slide #3: Human Epididymis

Visible in this picture is the Ductus Deferens; Visible prominently are the concentric circular smooth muscle layer (interior) and longitudinal smooth muscle layer (exterior)


47). Prostate gland (H & E)

Slide #3: Human Prostate Gland

Visible in this picture is the fibromuscular stroma (pink) and the secretory elements (parenkyma) which are purplish colour;


48). Ovary (H & E)

Slide #5: Ovary

Visible in this slide are (from top left of slide down to bottom right): Primordial follicles next to 1o follicles. This layer constitutes the germinative epithelium. The large follicle seen in the centre of the slide is a 2o Follicle, which is internally surrounded by purple stained by Granulosa Cells. Right beneath it are visible a couple of Atretic ova.


Slide #5: Ovary

Visible centrally in this slide is: A 2o follicleH & E Staining(Medium Magnification)

Slide #5: Ovary

Visible in this slide is: A Graafian follicle Personal Note: The follicle is so massive that it takes up the entire field of view, and more, of the slide!


Slide #5 (2nd one): Ovary

Visible in this slide is: One massive Corpus Luteum is seen during pregnancy in women in their ovaries.


49). Oviduct (H & E)

Slide #6: Oviduct

Visible in this slide is (from top right to bottom left): Connective tissue and smooth muscle layer. The finger-like projections are simple ciliated columnar epithelium, and a covering layer of cilia.


Slide #6: Oviduct

Visible in this slide is: The finger-like projections are simple ciliated columnar epithelium, and a covering layer of cilia which is easily visible.


50). Uterus (H & E)

Slide #6: Uterus

Visible in this slide is (from top right to bottom left): The exterior perimetrium, the middle layer of myometrium (composed of a muscle layer of circular, spiral, and longitudinal fibers), and the internal endometrium layer (lined with mucosa, lamina propria, and a lining epithelium composed of simple columnar epithelium).


Uterus (mouse)

51). Spinal cord (Stained with osmium and eosin or H & E)

Slide #3Spinal Cord

Visible: gray matter, white matter, anterior roots, posterior roots, motor nerve cells The dark stained regions are the grey matter (to the right of the field pf view), and the dark visible nuclei are the motor neurons in the ANTERIOR ROOTS

Stained w/Osmic Acid and Eosin

Slide #3Spinal Cord

Visible in this picture is the Spinal CanalStained w/Osmic Acid and Eosin

Slide #3Spinal Cord

Note: Posterior region. The neurons are much smaller and much less presentStained w/Osmic Acid and Eosin

52). Cerebellum (Silver impregnation)

Slide #2:CerebellumStained with Silver Impregnation

52). b. Cerebellum (H & E)

Slide #2:Cerebellum

Visible is the Medullary Core (nerve fibers); Visible in the darker, centre region is the Granular layer, with the Purkinje cell layer at the periphery of this layer. The lighter pink stained region is the White Matter.

Note: The Purkinje Cells are right at the border of the Granular layerH & E Staining

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Slides for Histo Practical Exam