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StockP04 2655 LMD SERIES _ LMD series describes the process of laser microdissection using the Lecia AS/LMD®.

slide show pdf - National Institute of Environmental …Tissueon slide after cutting is complete. Sampleis dropped into tube cap for lysing. Title slide_show_pdf Created Date 5/24/2007

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Page 1: slide show pdf - National Institute of Environmental …Tissueon slide after cutting is complete. Sampleis dropped into tube cap for lysing. Title slide_show_pdf Created Date 5/24/2007

StockP04 2655

LMD SERIES

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LMD series describes the process of laser microdissection using the Lecia AS/LMD®.

Page 2: slide show pdf - National Institute of Environmental …Tissueon slide after cutting is complete. Sampleis dropped into tube cap for lysing. Title slide_show_pdf Created Date 5/24/2007

AS/LMD instrument with monitor. _

Page 3: slide show pdf - National Institute of Environmental …Tissueon slide after cutting is complete. Sampleis dropped into tube cap for lysing. Title slide_show_pdf Created Date 5/24/2007

Fully automated controller to guide microscope objectives, stage and lights. _

Page 4: slide show pdf - National Institute of Environmental …Tissueon slide after cutting is complete. Sampleis dropped into tube cap for lysing. Title slide_show_pdf Created Date 5/24/2007

Specially prepared cryosections on foil slides are used. Invert the slide and lock into holder.

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Page 5: slide show pdf - National Institute of Environmental …Tissueon slide after cutting is complete. Sampleis dropped into tube cap for lysing. Title slide_show_pdf Created Date 5/24/2007

High throughput microdissection can be performed with four tubes in place. _

Page 6: slide show pdf - National Institute of Environmental …Tissueon slide after cutting is complete. Sampleis dropped into tube cap for lysing. Title slide_show_pdf Created Date 5/24/2007

Tube carrier is loaded underneath microscope stage for automation. _

Page 7: slide show pdf - National Institute of Environmental …Tissueon slide after cutting is complete. Sampleis dropped into tube cap for lysing. Title slide_show_pdf Created Date 5/24/2007

Actual laser cutting can be seen on the monitor. _

Page 8: slide show pdf - National Institute of Environmental …Tissueon slide after cutting is complete. Sampleis dropped into tube cap for lysing. Title slide_show_pdf Created Date 5/24/2007

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Tissue on slide after cutting is complete. Sample is dropped into tube cap for lysing. _

Page 9: slide show pdf - National Institute of Environmental …Tissueon slide after cutting is complete. Sampleis dropped into tube cap for lysing. Title slide_show_pdf Created Date 5/24/2007

StockP04 2655

MMI SERIES

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MMI series describes the process of laser microdissection using the CellCut technology.

Page 10: slide show pdf - National Institute of Environmental …Tissueon slide after cutting is complete. Sampleis dropped into tube cap for lysing. Title slide_show_pdf Created Date 5/24/2007

New instrumentation from Molecular Machines & Industries; CellCut® with touch monitor. _

Page 11: slide show pdf - National Institute of Environmental …Tissueon slide after cutting is complete. Sampleis dropped into tube cap for lysing. Title slide_show_pdf Created Date 5/24/2007

,• Cut\rlrdth11152006 TIF - Nero PhotoS nap Viewer , , -·· "'' ·-'-'

NEHS ICU Ot -06087 Pat Stockton Experrnerial Pettlology Rm • C-248

40 ()bjec:ti>te Cut Wclh • ae&.m Speed • •7% Focus • 56% Power • 56'%

20X oo,ectJve ~·132!Un

Speed • SO'J. F oeus • ~ Po-....,.er • ~

1 ox oc,ectJve Ct.4 Wclh • 2 3&.m Speed • 50% Focus • 43% Power • 69'1.

I Novetrber 15, 2006

by Jr:h1 Serw::e r 11M , he

UV laser may be focused to cut below 1 micron for very focused sample isolation. _

Page 12: slide show pdf - National Institute of Environmental …Tissueon slide after cutting is complete. Sampleis dropped into tube cap for lysing. Title slide_show_pdf Created Date 5/24/2007

Cut samples are extracted by cap touch isolation. _

Page 13: slide show pdf - National Institute of Environmental …Tissueon slide after cutting is complete. Sampleis dropped into tube cap for lysing. Title slide_show_pdf Created Date 5/24/2007

Sterile consumables ensure molecular integrity. _

Page 14: slide show pdf - National Institute of Environmental …Tissueon slide after cutting is complete. Sampleis dropped into tube cap for lysing. Title slide_show_pdf Created Date 5/24/2007

StockP04 2655

DNA

RNA

Protein

3 Steps to Lyse Material in Preparation for Molecular Analysis

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Tissue on slide after cutting is complete. Sample is dropped into tube cap for lysing.