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Microbiological view of tuberculin test and animal inoculation for diagnosis
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Skin test & Animal Inoculation
Skin Test
• All patients with FUO are tested for TB with Mantoux test (Tuberculin test)
• Other FUO causing diseases like Histoplasmosis, coccidiomycosis, sarcoidosis can also be tested.
Mantoux Test
• 0.1 ml of PPD containing 5TU is taken.• Injected intradermally on flexor aspect of
forearm• Examined after 48-72 hours, Induration
measured at its widest point• Result: ≥10mm positive, 6-9mm equivocal, ≤5
mm negative
ANIMAL INOCULATION
• Done mainly for isolating viruses• Not all viruses have an animal model
ANIMAL INOCULATION CONTD.
• ANIMAL INOCULATION– Animals used:
Monkey, mice, hamster, dog, chick embryo, guinea pig, rabbits, ferrets
– Routes of inoculation: Intra cerebral Subcutaneous Intra peritoneal Intra nasal Intra venous
– Disadvantages Immunity may interfere with viral growthAnimal may harbour latent viruses
ANIMAL INOCULATION CONTD..
S. No Virus Animal Model Inoculation Look For
1. Colorado tick fever Suckling Mice Intracerebral Fatal Encephalitis
2. Coxsackievirus Suckling Mice Intraperitoneal, Subcutaneous, Intracerebral
Histopathology in Infected Mice
3. Hepatitis A Chimpanzee & marmosets
Intravenous Hepatitis
4. Hepatitis E Chimpanzee & macaque
Intravenous Hepatitis
5. LCMV Adult Mice Intracerebral Flourescent antibody staining of brain
6. Poliovirus Monkeys Intracerebral & Intraspinal
Paralytic Poleomylitis