SKIN ENERGY SUPPLEMENT - QuickBox · Barnet Products Corporation140 Sylvan Avenue Englewood Cliffs NJ 07632 Tel 201 346 4620 Fax 201 346 4333 Web barnetproducts.com Presents THIOTAINE

Barnet Products Corporation 140 Sylvan Avenue Englewood Cliffs NJ 07632Tel 201 346 4620 Fax 201 346 4333 Web barnetproducts.com

Presents

THIOTAINE DOSSIER

SKIN ENERGY SUPPLEMENT

INCREASING OXYGEN USE & ENERGYFROM MITOCHONDRIA

THIOTAINE

AGI Dermatics

The information contained in this technical bulletin is, to the best of our knowledge, true and accurate. No warranty, expressed or implied is made orintended. The use should be based upon the customer’s own investigations and appraisal. No recommendation should be construed as an induce-ment to use a material in infringement of patents or applicable government regulations.

THIOTAINE DOSSIER - 1

INTRODUCTION

OXYGEN, ENERGY & WELL-BEING

PART ONE: Thiotaine and Mitochondriaa) The Powerhouse: Mitochondriab) Thiotaine: Natural Amino Acid

PART TWO: The Dual Role of Thiotainea) Role in Energy Production and Fatty Acid Transportb) Role in Anto-Oxidation and Mitochondria Protection

PART THREE: Thiotaine, Anti-Stress Moleculea) Reducing Solar Stressb) Reducing Oxidative Stress

PART FOUR: Thiotaine, A Clarifying Molecule

CONCLUSION

BIBLIOGRAPHY


INTRODUCTION: OXYGEN, ENERGY AND WELL-BEING

Energy is the moving force of life. It is a fundamental and indispensible element in cel-lular activity. All cells must produce energy to survive, and oxygen consumption is fun-damental to the process.

In our daily life we associate oxygen with “outdoor activity.” We go to the mountains forfresh air. In trendy shopping areas we can visit oxygen bars. Oxygen is associated withhealth, with looking and feeling good.

It is also known that Olympic long-distance runners train in the mountains to increase theoxygen levels in their blood to help give them a competitive edge.

Energy and oxygen are related and Thiotaine is a molecule that helps assure efficientuse of oxygen for more efficient energy production. This action also assures the well-being of the cells.


PART ONE: THIOTAINE AND MITOCHONDRIA

a) Power House and Energy Production

ATP (Figure 2) is an energy transporter in the cell (Figure 1). As a result of its high ener-gy bonds, it releases energy during its hydrolysis according to the following transforma-tion:

As a common denominator between catabolism and biosynthesis, the ATP level is a faith-ful indicator of physiological dynamics.

The cells draw energy from the metabolizing of carbohydrates, lipids and proteins in orderto synthesize ATP (see below). ATP - a universal cellular fuel - participates in all inter-cellular reactions of biosynthesis (keratin, ceramides), and in the transport of substancesacross membranes as well as in the exchanges between the cells and their environment.So the cells incorporate nutrients more easily and discharge their metabolic wastes.Thus, the epidermis is cleansed.

ATP is made in special organelles in the cells called mitochondria. ATP is synthesizedfrom AMP and phosphate by burning faty acids with oxygen, called aerobic metabolism.To increase ATP production it is important to increase the presence of fatty acids in themitochondria. Thiotaine is a molecule that transfers fatty acids into the mitochondria tohelp use oxygen efficiently and to produce more energy.

Without mitochondria, energy is generated by anaerobic metabolism, which is inefficientand generates toxic wastes.

Figure 1

Figure 2: ATP Chemical Structure



b) Thiotaine - Natural Amino Acid

Thiotaine is the trade name for pre-solubilized L-ergothioneine (Figure 3).

Figure 3: L-ergothioneine chemical structure

* L-ergothioneine can be found in botanicals such as mushrooms, vegetables and grain.

* L-ergothioneine is a dietary requirement.

* L-ergothioneine is concentrated in red blood cells to protect hemoglobin.

* Unlike many molecules with a Thiol group, Thiotaine is odorless, tasteless, water-sol-uble and stable.

* Thiotaine has two functional groups:

---a transferring group: N-(CH3)3 (red dotted line)

---an anti-oxidant group: Thiol

N N

S

CH2-CH-COO-

N+(CH3)3


PART TWO - THE DUAL ROLE OF THIOTAINEa) Role in Energy Production and Fatty Acid Transport

The transferring group of Thiotaine -

CH3 - N+ - CH3

CH3

- is also present in Carnitine

Carnitine is used in slimming products. It helps to transfer fatty acid in the mitochon-dria and the oxygen present will burn the fatty acid.

Carnitine’s mode of action is described below.

Fatty Acids+ Co ASH

(CH2) COOH

Fatty Acyl+ Carnitine

Co A

Fatty Acyl+ Co ASH

Carnitine

Fatty acylcarnitine + Co ASH

Fatty acids CoA+ Carnitine

Fatty acid + O2 CO2 + ATP

O (oxygen radical)

Consequence: Less Fatty Acid; More Energy


Transfer intoMitochondria

majority

minority


Thiotaine’s mode of action is similar.

Fatty alcyl CoA + Thiotaine

Fatty acyl Thiotaine + Co ASH

Consequence: Less Fatty Acid, More Energy

But Also: Control of Oxidative Stress by the second group of Thiotaine: The Thiol

Penetrates theMitochondria

Fatty acyl Thiotaine + Co ASH

Fatty acids CoA + Thiotaine

Fatty acid + O2 CO2 + ATP

O*

control of oxidative stress


Thiotaine will shift fatty acids in mitochondria to increase the aerobic metabolism exist-ing in the mitochondria.


PROTOCOL

Thiotaine, like carnitine, pushes the fatty acid into the mitochondria. The fatty acids are"burned" with oxygen and the result is the production of energy (ATP). With Thiotaine,this activity is increased by 16.5%


The Anti-Oxidant Group of Thiotaineb) Role in Anti-Oxidation and Mitochondria Protection

S - H

Oxygen is good for metabolism and ATP synthesis But it also counteracts the side-effect of oxygen metabolism, which is the production of free radicals.

Thiotaine has a Thiol group; therefore, it has anti-oxidant properties.

Antioxidant PowercheckAll of the above molecules (except MgAscPO4) contain Thiol antioxidant activity. Most

of them have a strong characteristic odor. None perform as well as Thiotaine when chal-

lenged with an oxidizing treatment (in this case, 20 µM alloxan).

0

20

40

60

80

100

120N-acCys

L-Met

BME

BHT

L-Cys

MgAscPO4

Thiotaine

Lipid Peroxides % Control


Thiotaine is an antioxidant, protecting the mitochondrial membrane against oxidation.(Note: Alloxan is an oxidant)

Thiotaine:Mitochondrial Protection

CONCLUSION

We have demonstrated that Thiotaine not only participates in the consumption of oxygento produce energy, but Thiotaine also acts as an anti-oxidant, especially as a protectorof the mitochondria.

The hemoglobin molecule, a carrier of oxygen in the blood, uses Ergothioneine as a pro-tector.

Mouse keratinocytes stainedwith mitochondrial dye JC-1Mitochondria are healthy.

Cells treated with alloxan,killing mitochondria withoxygen radicals. The yellowcolor indicates the death ofmitochondria due tooxidation.

Cells treated with Thiotaineand alloxan are protected.The mitochondria are notdamaged by the oxidant.


PART THREE: THIOTAINE: ANTI-STRESS MOLECULE

In this section we will discuss two different expressions of stress. a) UV-B Stress

UV induces damage to many parts of the cell. The skin responds to this stress by releas-ing signals called cytokines. TNF-Alpha (Tumor Necrosis Factor) is one such type.These cytokines cause the inflammation of skin that follows stress.

Thiotaine reduces TNF-Alpha, a stress signal cytokine

Thiotaine®

Reduces Stress Signals

0

20

40

60

80

100

120

Per

cent

TN

Falp

ha In

duct

ion

UV-B100

J/m2

UV+1%Thiotaine


The second approach is to consider the consequences of oxidation.

b) Inflammation

Elastase (the destroyer of elastin) is under the control of a group of enzymes called Alpha Anti-Proteinase-1. Protecting the AP-1 is like preserving the elastin and avoidingaging.

HO CL destroys Alpha Anti-Proteinase-1; therefore, elastase is activated and the agingprocess begins.

Thiotaine is a HO CL chelator, amplifying the power of Alpha Anti-Proteinase-1 as ananti-elastase material (as shown on the next page).

Inflammation, even if it is not easily seen, causes attacking cells to enter the skin. Thesemacrophages cause the release of enzymes, like elastase, that destroy the supportingstructure of skin. This leads to the appearance of aging skin.

Thiotaine controls the release of elastase during an inflammatory attack. It preserves theskin defenses, especially the protein Alpha AP-1, the primary defense against elastase.

OXIDATION

ACTIVATION OF MACROPHAGES

RELEASE OF HO CLRELEASE OF ELASTASE



PART FOUR: THIOTAINE AS A CLARIFIER

Clarifier:

Thiotaine acts like a clarifier because it is:

* A metal chelator like Kojic Acid* An antioxidant like Vitamin C * It inhibits tyrosinase and also inhibits melanin in cell cul

ture at 1% use level

0

1

2

3

4

5

Mel

anin

(pg

/cel

l)

None Melanin Induction Plus Thiotaine

Documents

SKIN ENERGY SUPPLEMENT - QuickBox · Barnet Products Corporation140 Sylvan Avenue Englewood Cliffs NJ 07632 Tel 201 346 4620 Fax 201 346 4333 Web barnetproducts.com Presents THIOTAINE