Signal Transduction Laboratory-2 Lab Page

Complete list of Publications and Patents A. Peer-Reviewed Original Research Articles (# denotes publication as the corresponding author) # 1. Tripathi V, Agarwal H, Priya S, Batra H, Modi P, Pandey M, Saha D, Raghavan SC, Sengupta S (2018). MRN complex-dependent recruitment of ubiquitylated BLM helicase to DSBs negatively regulates DNA repair pathways. Nat Commun. 9(1):1016. PMID: 29523790. 2. Sreekanth V, Medatwal N, Kumar S, Pal S, Vamshikrishna M, Kar A, Bhargava P, Naaz A, Kumar N, Sengupta S, Bajaj A (2017). Tethering of Chemotherapeutic Drug/Imaging Agent to Bile Acid-Phospholipid Increases the Efficacy and Bioavailability with Reduced Hepatotoxicity. Bioconjug Chem. 28(12):2942-2953. PMID: 29083862. 3. Sreekanth V, Medatwal N, Pal S, Kumar S, Sengupta S, Bajaj A (2017). Molecular Self-Assembly of Bile Acid-Phospholipids Controls the Delivery of Doxorubicin and Mice Survivability. Mol Pharm. 14(8):2649-2659. PMID: 28665132. # 4. Kumari J, Hussain M, De S, Chandra S, Modi P, Tikoo S, Singh A, Sagar C, Sepuri NB, Sengupta S (2016). Mitochondrial functions of RECQL4 are required for the prevention of aerobic glycolysis dependent cell invasion. J Cell Sci. 129(Pt 7): 1312-18. PMID: 26906415 # 5. Kharat SS, Tripathi V, Damodaran AP, Priyadarshini R, Chandra S, Tikoo S, Nandhakumar R, Srivastava V, Priya S, Hussain M, Kaur S, Fishman JB, Sengupta S (2016). Mitotic phosphorylation of Bloom helicase at Thr182 is required for its proteasomal degradation and maintenance of chromosomal stability. Oncogene 35(8): 1025-38. PMID: 26028025 6. Singh M, Bansal S, Kundu S, Bhargava P, Singh A, Motiani RK, Shyam R, Sreekanth V, Sengupta S, Bajaj A (2015). Synthesis, structure-activity relationship, and mechanistic investigation of lithocholic acid amphiphiles for colon cancer therapy. Med. Chem. Commun., 6: 192-201. PMID: 25685308

# 7. Singh M, Kundu S, Reddy MA, Sreekanth V, Motiani RK, Sengupta S*, Srivastava A*, Bajaj A* (2014). Injectable small molecule hydrogel as a potential nanocarrier for localized and sustained in vivo delivery of doxorubicin. Nanoscale 6(21): 12849-55 (* denotes co-corresponding authors). PMID: 25227567

8. Rajanala K, Sarkar A, Jhingan GD, Priyadarshini R, Jalan M, Sengupta S, Nandicoori VK (2014). Phosphorylation of nucleoporin Tpr governs its differential localization and is required for its mitotic functions. J Cell Sci. 127(Pt 16): 3505-20. PMID: 24938596

# 9. Gupta S, De S, Srivastava V, Hussain M, Kumari J, Muniyappa K, Sengupta S (2014) RECQL4 and p53 potentiate the activity of polymerase and maintain the integrity of the human mitochondrial genome. Carcinogenesis 35(1): 34-45. PMID: 24067899

10. Sreekanth V, Bansal S, Motiani RK, Kundu S, Muppu SK, Majumdar TD, Panjamurthy S, Sengupta S, Bajaj A (2013) Design, Synthesis, and Mechanistic Investigations of Bile acid-Tamoxifen Conjugates for Breast Cancer Therapy. Bioconjug Chem. 24(9): 1468-1484. PMID: 23909664

# 11. Chandra S, Priyadarshini R, Madhavan V, Tikoo S, Hussain M, Mudgal R, Modi P, Srivastava V, Sengupta S (2013) Enhancement of c-Myc degradation by BLM helicase leads to delayed tumor initiation. J Cell Sci. 126(Pt 16): 3782-3795. PMID: 23750012 # 12. Tikoo S, Madhavan V, Hussain M, Miller ES, Arora P, Zlatanou A, Modi P, Townsend K, Stewart GS*, Sengupta S* (2013) Ubiquitin-dependent recruitment of the Bloom Syndrome helicase in response to replication stress is required to suppress homologous recombination. EMBO J. 32(12): 1778-1792 (* denotes co-corresponding authors). PMID: 23708797 13. Jadav RS, Chanduri MV, Sengupta S, Bhandari R (2013) Inositol Pyrophosphate Synthesis by Inositol Hexakisphosphate Kinase I is Required for Homologous Recombination Repair. J Biol Chem. 288(5): 3312-3321. PMID: 23255604 14. Kumar A, Tikoo S, Maity S, Sengupta S, Sengupta S, Kaur A, Bachhawat AK (2012) Mammalian proapoptotic factor ChaC1 and its homologues function as -glutamyl cyclotransferases acting specifically on glutathione. EMBO Rep. 13(12): 1095-1101. PMID: 23070364 # 15. De S, Kumari J, Mudgal R, Modi P, Gupta S, Futami K, Goto H, Lindor NM, Furuichi Y, Mohanty D, Sengupta S (2012) RECQL4 is essential for the transport of p53 to mitochondria in normal human cells in the absence of exogenous stress. J Cell Sci. 125(Pt 10): 2509-2522. PMID: 22357944 # 16. Kaur S, Modi P, Srivastava V, Mudgal R, Tikoo S, Arora P, Mohanty D, Sengupta S (2010) Chk1-dependent constitutive phosphorylation of BLM helicase at Serine 646 decreases after DNA damage. Mol Cancer Res. 8(9): 1234-1247. PMID: 20719863 17. Larrieu D, Ythier D, Binet R, Brambilla C, Brambilla E, Sengupta S, Pedeux R (2009) ING2 controls DNA replication forks progression to maintain genome stability. EMBO Rep. 10(10): 1168-1174. PMID: 19730436 18. Mehta S, Miklos I, Sipiczki M, Sengupta S, Sharma N (2009) The Med8 mediator subunit interacts with the Rpb4 subunit of RNA polymerase II and Ace2 transcriptional activator in Schizosaccharomyces pombe. FEBS Lett. 583(19): 3115-3120. PMID: 19720063 # 19. Srivastava V, Modi P, Tripathi V, Mudgal R, De S, Sengupta S (2009) BLM helicase stimulates the ATPase and chromatin remodeling activities of RAD54. J Cell Sci. 122(Pt 17): 3093-3103. PMID: 19671661

# 20. Tripathi V, Kaur S, Sengupta S (2008) Phosphorylation-dependent interactions of BLM and 53BP1 are required for their anti-recombinogenic roles during homologous recombination. Carcinogenesis 29(1): 52-61. PMID: 17984114 # 21. Tripathi V, Nagarjuna T, Sengupta S (2007) BLM helicase-dependent and independent roles of 53BP1 during replication stress mediated homologous recombination. J. Cell Biol. 178(1): 9-14. PMID: 17591918 22. Pedeux R, Sengupta S, Shen JC, Demidov ON, Saito S, Onogi H, Kumamoto K, Wincovitch S, Garfield SH, McMenamin M, Nagashima M, Grossman SR, Appella E, Harris CC (2005) ING2 regulates the onset of replicative senescence by induction of p300-dependent p53 acetylation. Mol Cell Biol. 25(15): 6639-6648. PMID: 16024799 23. Zhang R, Sengupta S, Yang Q, Linke SP, Yanaihara N, Bradsher J, Blais V, McGowan CH, Harris CC (2005) BLM helicase facilitates Mus81 endonuclease activity in human cells. Cancer Res. 65(7): 2526-2531. PMID: 15805243 24. Sengupta S, Shimamoto A, Koshiji M, Pedeux R, Rusin M, Spillare EA, Shen JC, Huang LE, Lindor NM, Furuichi Y, Harris CC (2005) Tumor suppressor p53 represses transcription of RECQ4 helicase. Oncogene 24(10): 1738-1748. PMID: 15674334 25. Sengupta S, Robles AI, Linke SP, Sinogeeva NI, Zhang R, Pedeux R, Ward IM, Celeste A, Nussenzweig A, Chen J, Halazonetis TD, Harris CC (2004) Functional interaction between BLM helicase and 53BP1 in a Chk1-mediated pathway during S-phase arrest. J. Cell Biol. 166(6): 801-813. PMID: 15364958 26. Yang Q, Zhang R, Wang XW, Linke SP, Sengupta S, Hickson ID, Pedrazzi G, Perrera C, Stagljar I, Littman SJ, Modrich P, Harris CC (2004) The mismatch repair heterodimer, hMSH2/6, regulates BLM helicase. Oncogene 23(21): 3749-3756. PMID: 15064730 27. Hussain SP, Amstad P, He P, Robles A, Lupold S, Kaneko I, Ichimiya M, Sengupta S, Mechanic L, Okamura S, Hofseth LJ, Moake M, Nagashima M, Forrester KS, Harris CC (2004) p53-induced upregulation of MnSOD and GPx, but not catalase increases oxidative stress and apoptosis. Cancer Res. 64(7): 2350-2356. PMID: 15059885 28. Linke SP, Sengupta S, Khabie N, Jeffries BA, Buchhop S, Miska S, Henning W, Pedeux P, Wang XW, Hofseth LJ, Yang Q, Garfield SH, Strzbecher HW, Harris CC (2003) p53 interacts with hRAD51 and hRAD54, and directly modulates homologous recombination. Cancer Res. 63(10): 2596-2605. PMID: 12750285 29. Sengupta S, Linke SP, Pedeux R, Yang Q, Farnsworth J, Garfield SH, Valerie K, Shay JW, Ellis NA, Wasylyk B, Harris CC (2003) BLM helicase-dependent transport of p53 to sites of stalled DNA replication forks modulates homologous recombination. EMBO J. 22(5): 1210-1222. PMID: 12606585 30. Ganguli G, Back J, Sengupta S, Wasylyk B (2002) The p53 tumour suppressor inhibits glucocorticoid receptor induced proliferation of erythroid progenitors. EMBO Rep. 3(6): 569-574. PMID: 12034755

31. Sengupta S, Wasylyk B (2001) Ligand dependent interaction of the glucocorticoid receptor with p53 enhances their degradation by Hdm2. Genes Dev. 15(18): 2367-2380. PMID: 11562347 32. Sengupta S, Vonesch JL, Waltzinger C, Zheng H, Wasylyk B (2000) Negative cross-talk between p53 and the glucocorticoid receptor and its role in neuroblastoma cells. EMBO J. 19(22): 6051-6064. PMID: 11080152 33. Sengupta S, Ralhan R, Wasylyk B (2000) Tumour regression in a ligand inducible manner mediated by a chimeric tumour supressor derived from p53. Oncogene 19(3): 337-350. PMID: 10656680 # 34. Sengupta S, Shaila MS, Rao GR (1997) A novel phosphorylation mediated regulation of nitrite reductase in Candida utilis. FEBS Lett. 416(1): 51-56. PMID: 9369231 # 35. Sengupta S, Shaila MS, Rao GR (1997) In vitro and in vivo regulation of assimilatory nitrite reductase from Candida utilis. Arch Microbiol. 168(3): 215-224. PMID: 9547218 36. Sengupta S, Shaila MS, Rao GR (1996) Purification and characterisation of assimilatory nitrite reductase from Candida utilis. Biochem J. 317(Pt 1): 147-155. PMID: 8694757 B. Peer Reviewed Review Articles # 1. Tikoo S, Sengupta S (2010). Time to Bloom. Genome Integr. 1(1): 14. PMID: 21050475

2. Sengupta S, Harris CC (2005). p53: traffic cop at the crossroads of DNA repair and recombination. Nature Rev. Mol. Cell Biol. 6(1): 44-55. PMID: 15688066

3. Sengupta S, Wasylyk B (2004) Physiological and pathological consequences of the interactions of the p53 tumor suppressor with the glucocorticoid, androgen and estrogen receptors. Ann NY Acad Sci. 1024: 54-71. PMID: 15265773 C. Conference Proceedings # 1. Sengupta (2015). Mechanisms of maintenance of genomic stability by BLM and RECQL4 helicases. Int J Mol Med. 36, S102. 2. Guerillon C, Larrieu D, Mourcin F, Brambilla C, Sengupta S, Pedeux R. (2012). The Tumor Suppressive Protein ING2 is Required for DNA Damage Response Proteins Recruitment and Promotes NHEJ. Eur J Cancer. 48, suppl. 5 S25S288. # 3. Tripathi V, Kaur S, Nagarjuna T, Sengupta S (2008). Role of BLM and 53BP1 during homologous recombination. Anticancer Res. 28(5C): 3157-3556.

4. Sengupta S, Robles A, Linke S; Sinogeeva N; Zhang R, Pedeux R, Ward I, Celeste A, Nussenweig A, Chen J, Halazonetis T, Harris C (2004). BLM helicase and 53BP1 cooperate in the replication stress pathway. Toxicol Pathol., 32: 749761. 5. Hussain SP, Amstad P, He P, Robles A, Lupold S, Sengupta S, Ichimiya M, Okamura S, Moake M, Forrester K, Harris CC (2004). P53-mediated oxidative stress and apoptosis. Toxicol Pathol., 32(1): 163-164. D. Patents 1. Conjugated anti-proliferative drug nano-particles and process for preparation thereof. (PCT application number PCT/IN2017/050253). 2. RECQL4, the DNA helicase mutated in rothmund-thomson syndrome, regulated mithochondrial function (European application no. 10793297.2 accepted on 10/11/2014, Filed in India 2321/DEL/2009, PCT application number PCT/IB2010/002834, US National Phase Application Serial No. 13/318,667)

Past and Present Collaborators

Anand Bachhawat (IISER, Mohali) Archana Singh (CSIR-IGIB, Delhi) Arnab Mukhopadhyay (NII, New Delhi) Avinash Bajaj (THSTI, Gurgaon) Chandrasekhar Sagar (NIMHANS, Bengaluru) Debasisa Mohanty (NII, New Delhi) Grant Stewart (University of Birmingham, UK) Isabelle Gross (INSERM U1113, Strasbourg, France) Jean-Noel Freund (INSERM U1113, Strasbourg, France) Jyothi Prabhu (St. Johns Research Institute, Bengaluru) K. Muniyappa (IISc, Bengaluru) Naresh Babu V. Sepuri (University of Hyderabad, Hyderabad) Nimisha Sharma (Indraprastha University, Delhi) Noralane Lindor (Mayo Clinic, USA) Patrick Legembre (Universite de Rennes, Rennes, France) Rashna Bhandari (CDFD, Hyderabad) Remy Pedeux (INSERM U917, Rennes, France) Sathees Raghavan (IISc, Bengaluru) Shantanu Chowdhury (CSIR-IGIB, Delhi) Subeer Majumdar (NII, New Delhi) S.V.S. Deo (AIIMS, New Delhi) Tapas Kumar Kundu (JNCASR, Bengaluru) Ullas Kolthur Seetharam (TIFR, Mumbai) Vinay Nandicoori (NII, India) Yasuhiro Furuichi (GeneCare Research Institute, Japan)

Past and Present Funding National Institute of Immunology (NII), India Department of Biotechnology (DBT), India Department of Science and Technology (DST), India Science and Engineering Research Board (SERB), India Council of Scientific and Industrial Research (CSIR), India Indo-French Centre for the Promotion of Advanced Research (IFCPAR) National Institutes of Health (NIH), USA

Present Lab Members

INSPIRE Faculty

Name Information

Ekjot Kaur (2017 onwards)

Scientific interest: Chemoresistance, cancer therapy, CRISPR, microRNA Email: ktojke@gmail.com Website: https://www.researchgate.net/profile/Ekjot_Kaur2

DBT Postdoctoral Fellow

Name Information

Vivek Tripathi (2005-2008)

(2013 onwards)

Scientific interest: DNA damage response, DNA repair pathways, protein turnover, p53 biology Email: vivek@nii.ac.in Website: https://www.researchgate.net/profile/Vivek_Tripathi10

Research Associate

Name Information

Swati Priya (2012 onwards)

Scientific interest: DNA damage response, microRNA biology, in vivo animal model Email: swati_priya@nii.ac.in

Graduate Students (NII)

Name Information

Himanshi Agarwal

(2013 onwards)

Scientific interest: DNA damage response, phosphorylation studies, mass spectrometry Email: himanshiagarwal@nii.ac.in Website: https://www.researchgate.net/profile/Himanshi_Agarwal

Preeti Attri (2014 onwards)

Scientific interest: DNA damage response, epigenetic signalling, degradation of oncogenes Email: preeti@nii.ac.in

Aamir Khan (2015 onwards)

Scientific interest: Mitochondrial homeostasis Email: aamir.khan@nii.ac.in

Sayan Chakraborty (2016 onwards)

Scientific interest: Mice models for carcinogenesis Email: sayan@nii.ac.in

Graduate Student (External)

Name Information

Mansoor Hussain

(2012 onwards)

Scientific interest: Mitochondrial replication, mitochondrial protein entry, post-translational modifications of proteins Email: mansoormarrow@gmail.com Website: https://www.researchgate.net/profile/Mansoor_Hussain3

Project Fellow

Name Information

Swati Kulshrestha

(2016 onwards)

Scientific interest: microRNA biology, p53 biology Email: kulswat@gmail.com

Support Staff

Name Information

Radhey Shyam

(2007 onwards)

Scientific interest: Animal maintenance

Nitin Kumar (2012 onwards)

Scientific interest: Animal maintenance, genotyping Email: minick196@gmail.com

Graduate Students (NII)

Name Information

Sarabpreet Kaur (2004-2009)

Postdoctoral Fellow, Case Western Reserve School of Medicine, USA

Suruchika Chandra (2005-2011)

Email: suruchikachandra1@gmail.com Website: https://www.researchgate.net/profile/Suruchika_Chandra

Siddharth De (2006-2012)

Postdoctoral Research Associate, University of Cambridge, UK Email: siddharthde@gmail.com Website: https://www.researchgate.net/profile/Siddharth_De

Shweta Tikoo (2007-2012)

Postdoctoral Fellow, Centenary Institute, Australia Email: s.tikoo@centenary.org.au Website: https://www.researchgate.net/profile/Shweta_Tikoo

Graduate Students (NII)

Name Information

Suhas Kharat (2008-2014)

Postdoctoral Fellow, National Cancer Institute, USA Email: suhas.kharat@nih.gov Website: https://www.researchgate.net/profile/Suhas_Kharat

Jyoti Kumari (2009-2015)

Postdoctoral Fellow, Dartmouth College, USA Email: jyoti.sls.jnu@gmail.com Website: https://www.researchgate.net/profile/Jyoti_Kumari30

Raina Priyadarshini (2010-2015)

Senior Analyst at E Z BioXcel Solutions, Gurgaon, India Email: rpriya0387@gmail.com Website: https://www.researchgate.net/profile/Raina_Priyadarshini

Post-doctoral Fellows

Name Information

Harish Batra (2011-2013)

Postdoctoral Fellow, Regional Centre of Biotechnology, Faridabad, Haryana Email: harishbatra@gmail.com

Ramadass Nandhakumar (2013-2014)

Email: nandhakumarphd@gmail.com

Anjali Geethadevi (2016)

Postdoctoral Fellow, Medical College of Wisconsin, USA Email: anjie.sigma2@gmail.com

Project Fellows

Name Information

Richa Mudgal (2007-2008)

Postdoctoral Fellow, Institute for Research in Biomedicine(IRB), Barcelona, Spain Email: richamudgal@gmail.com Website: https://www.researchgate.net/profile/Richa_Mudgal

Prateek Arota (2008-2010)

Flow Cytometry Technical Assistant, IISER, Mohali, India Email: exquisiteprateek@gmail.com

Vivek Srivastava (2006-2011)

Assistant Professor, Sharda University, India Email: srivastavav13@gmail.com Website: https://www.sharda.ac.in/faculty-detail/dr--vivek-srivastava-466

Priyanka Modi (2007-2011)

Graduate student, Department of Zoology, Nagaland University, India Email: pmodi82@gmail.com Website: https://www.researchgate.net/profile/Priyanka_Modi

Project Fellows

Name Information

Shruti Gupta (2009-2011)

Graduate student, Symbiosis International University, Pune, India Email: shruti_bt05@yahoo.com Website: https://www.researchgate.net/profile/Shruti_Gupta22

Arun

Prasath Damodaran (2008-2010)

Graduate student, University of Rennes, France Email: dlarun88@gmail.com Website: https://www.researchgate.net/profile/Arun_Prasath_Damodaran2

Vinoth

Madhavan (2010-2016)

Email: s vinothmad1986@gmail.com Website: https://www.researchgate.net/profile/Vinoth_Madhavan

Visits due to collaborative projects

Name Information

Shreemoyee De (2016-2017)

Graduate student, Indian Institute of Technology, Delhi, India Email: shreemoyee19@gmail.com

Lab News

February 2018 Swati Priya defended her thesis on 12th February. Congratulations to Swati. Mansoor Hussain presented his findings via both poster and oral presentations in the 4th International Meeting on RECQ Helicases and Related Diseases (RECQ2018) which was held from 16th to 18th February in Chiba, Japan. He was offered a full travel grant by the organizers. Congratulations to Mansoor.

Opportunities in Lab

Depending on the availability of funded projects, the lab is on the lookout for JRFs, SRFs and Research Associates (RAs). For all positions the candidates have be exceptionally motivated, have a good working knowledge on the research areas of interest to the lab, have a pleasant personality and capable of putting in sustained effort for the project. The JRF/SRF candidates may be considered for external PhD. registration if they substantially contribute to atleast one publication from the lab. The RA candidates can be sponsored for the various National Post-doctoral Fellowships only if they are found to be exceptionally talented and want to work on the broad areas of interest to the lab. For contact email to sagar@nii.ac.in.

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