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Shipping Container Aquaponics: Life Cycle Assessment and comparison with conventional food production systems Marina Casades Cornet Bachelor Thesis for Bachelor of Engineering Degree Programme in Energy Technology Vaasa, Finland, 2020

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Page 1: Shipping Container Aquaponics: Life Cycle Assessment and

Shipping Container Aquaponics: Life Cycle

Assessment and comparison with conventional food

production systems

Marina Casades Cornet

Bachelor Thesis for Bachelor of Engineering

Degree Programme in Energy Technology

Vaasa, Finland, 2020

Page 2: Shipping Container Aquaponics: Life Cycle Assessment and

DEGREE THESIS

Author: Marina Casades Cornet

Degree Programme and Place: Mechanical Engineering, Vaasa

Specialization: Energy Technology

Supervisor: Philip Hollins

Title: Shipping Container Aquaponics: Life Cycle Assessment and comparison with

conventional food production systems.

Date: August 10, 2020 Number of pages: 44 Appendices: 2

Abstract

The continued and forecasted growth in food supply-demand has put increasing pressure

on the Earth’s ability to provide resources. New agriculture methods that assure to be more

sustainable are appearing as a solution. One of them is aquaponics, a combination of

hydroponics and aquaculture.

This thesis aims to undertake a full Life Cycle Analysis of an aquaponic system and

evaluate its sustainability by comparing it with conventional food production systems.

Firstly there was designed an aquaponic system to fit in a container. Afterwards, it was

evaluated their environmental impacts using SimaPro. Finally, it was compared the

sustainability significance between this aquaponics and traditional agriculture and

aquaculture systems.

The findings indicate that electricity and equipment proved to be the more significant

contributors to the impact categories. Although the extended use of shipping containers by

aquaponic companies, it demonstrated to be the most pollutant component in most

categories. Furthermore, LEDs contributed to more than 90% of all the energy impacts,

which could be dispensable in aquaponics with the use of natural lighting.

The system gave better results on the sustainability performance than conventional

agriculture and aquaculture carried on separately in most categories. It coincided with the

literature on the improvements in land use, eutrophication and the reduction of water

consumption, requiring just 14% of the water used in traditional agriculture. It differed

from previous articles in consuming 50% more energy than what was expected for an

aquaponic system, spending 130 times more energy than conventional agriculture.

Language: English Keywords: Life Cycle Assessment (LCA), sustainability,

aquaponics, agriculture, aquaculture

Page 3: Shipping Container Aquaponics: Life Cycle Assessment and

Table of contents

1. Introduction .................................................................................................................... 1

2. Purpose ........................................................................................................................... 1

3. Research ......................................................................................................................... 2

3.1 Theoretical starting points ...................................................................................... 2

3.1.1 Aquaponics ...................................................................................................... 2

3.1.2 Life Cycle Assessment .................................................................................... 3

3.1.3 Impact categories and endpoint characterization factors ................................. 3

3.2 Theoretical background .......................................................................................... 4

3.2.1 Testing setup .................................................................................................... 5

3.2.2 Previous studies ............................................................................................... 5

3.2.3 Characteristics of Vaasa .................................................................................. 5

4. Design of the aquaponic system ..................................................................................... 8

4.1 Container ................................................................................................................. 8

4.2 Hydroponic systems ................................................................................................ 8

4.2.1 Deep water culture (DWC) .............................................................................. 9

4.2.2 Nutrient film technique (NFT) towers ........................................................... 10

4.3 Plants ..................................................................................................................... 11

4.3.1 Lettuce characteristics and parameters .......................................................... 12

4.4 Fish ........................................................................................................................ 12

4.4.1 Yellow perch characteristics and parameters ................................................ 13

4.4.2 Fish ratios ...................................................................................................... 14

4.5 Fish food ............................................................................................................... 15

4.6 Fish tank ................................................................................................................ 16

4.7 Filtration ................................................................................................................ 16

4.7.1 Mechanical filtration...................................................................................... 16

4.7.2 Biological filtration ....................................................................................... 17

4.8 Pump ..................................................................................................................... 18

4.9 Water ..................................................................................................................... 19

4.10 Lights .................................................................................................................... 20

4.11 Controlling system ................................................................................................ 20

4.12 Final design ........................................................................................................... 20

5. Life Cycle Assessment of the aquaponic system ......................................................... 21

5.1 SimaPro and ReCiPe 2016 (H) ............................................................................. 21

Page 4: Shipping Container Aquaponics: Life Cycle Assessment and

5.2 Goal and scope definition ..................................................................................... 21

5.3 Inventory analysis ................................................................................................. 22

6. Results .......................................................................................................................... 23

6.1 Process tree and assembly groups ......................................................................... 23

6.2 Impact assessment ................................................................................................. 23

6.2.1 Characterization ............................................................................................. 24

6.2.2 Damage assessment ....................................................................................... 25

6.3 Impact assessment ignoring the effects of the shipping container ........................ 26

6.4 Comparison with conventional agriculture and aquaculture ................................ 28

6.4.1 Comparison with conventional agriculture.................................................... 28

6.4.2 Comparison with conventional agriculture combined with aquaculture ....... 30

7. Discussion .................................................................................................................... 32

8. Conclusions .................................................................................................................. 34

9. References .................................................................................................................... 35

10. Appendices ............................................................................................................... 45

Appendix 1 Data collection ............................................................................................. 45

Appendix 2 Results from SimaPro .................................................................................. 51

Page 5: Shipping Container Aquaponics: Life Cycle Assessment and

List of tables

Table 1: Required parameters for lettuce. ........................................................................... 12

Table 2: Required parameters for yellow perch. ................................................................. 13

Table 3: Length and weight of perch according to their age. .............................................. 15

Table 4: Average lengths and weights of the perch in the system according to their age. .. 15

Table 5: Each system performance to the impact categories............................................... 30

Table 6: Components ........................................................................................................... 45

Table 7: Manufacturing processes. ...................................................................................... 48

Table 8: Operation process. ................................................................................................. 49

Table 9. Transport................................................................................................................ 50

Table 10: Characterization by components and processes. ................................................. 51

Table 11: Characterization by assembly groups. ................................................................. 53

Table 12: Damage assessment by assembly group. ............................................................. 54

Table 13: Characterization comparison with and without shipping container. ................... 54

Table 14: Characterization by assembly groups without shipping container. ..................... 55

Table 15: Damage assessment by assembly group without shipping container. ................. 55

Page 6: Shipping Container Aquaponics: Life Cycle Assessment and

List of figures

Figure 1: Functioning of an aquaponic system ...................................................................... 2

Figure 2: Testing setup. ......................................................................................................... 5

Figure 3: Daylight and sunshine hours by month in Vaasa ................................................... 6

Figure 4: Maximum and minimum temperatures by month in Vaasa ................................... 6

Figure 5: Amount of municipal waste by treatment from 2013 to 2017 ............................... 7

Figure 6: Draft design of the aquaponic system (in mm). ..................................................... 9

Figure 7: Deep Water Culture System ................................................................................... 9

Figure 8: EDEN ISS interior of the container ..................................................................... 10

Figure 9: Design of the raft.................................................................................................. 10

Figure 10: Subjection of the plants and interior of the vertical towers ............................... 11

Figure 11: Design of the towers. ......................................................................................... 11

Figure 12: Lettuce and perch parameters comparison. ........................................................ 13

Figure 13: Order of calculations to follow when sizing an aquaponic system. ................... 14

Figure 14: Conical clarifier with baffles ............................................................................. 17

Figure 15: Design of the bioball used in the testing setup .................................................. 18

Figure 16: Heat height and flow rate chart from ActiveAQUA pumps .............................. 19

Figure 17: Inputs and outputs of the system. ....................................................................... 22

Figure 18: Process tree diagram of the life cycle of the aquaponic system and assembly

groups. ................................................................................................................................. 23

Figure 19: Characterization by assembly groups. ............................................................... 24

Figure 20: Temperature average comparison of Vaasa and Stevens Point ......................... 25

Figure 21: Damage assessment by assembly groups. .......................................................... 25

Figure 22: Relative contribution to the impact categories of the equipment with and

without the shipping container ignoring the effects of the waste scenario (%). .................. 26

Figure 23: Characterization graph by assembly group without including the container. .... 27

Figure 24: Damage assessment graph by assembly group without including the container.

............................................................................................................................................. 27

Figure 25: Water requirements comparison between conventional agriculture and

aquaponics. .......................................................................................................................... 28

Figure 26: Energy requirements comparison between conventional agriculture and

aquaponics. .......................................................................................................................... 29

Figure 27: Temperature average comparison between Vaasa and Yuma ........................... 29

Page 7: Shipping Container Aquaponics: Life Cycle Assessment and

Glossary

ABS Acrylonitrile Butadiene Styrene

DALY Disability-Adjusted Life Year

DWC Deep Water Culture

EPS Expanded polystyrene

GPH Gallons per hour

LCA Life Cycle Assessment

LED Light-Emitting Diode

NFT Nutrient Film Technique

PE Polyethylene

pH Potential for Hydrogen

PMMA Poly(metryl methacrylate)

PP Polypropylene

Ppm Parts-per-million

PVC Polyvinyl Chloride

Page 8: Shipping Container Aquaponics: Life Cycle Assessment and

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1. Introduction

With the development of the agricultural revolution 12,000 years ago, hunter-gatherer lives

radically changed. They abandoned their nomadic lifestyle to adopt a sedentary one. This

change allowed the development of huge human civilizations (Blakemore 2019). Back

then, the human population was around 4 million; increasing 1860-times until reaching the

7.8 billion people we are today. It is expected to achieve 8 billion people in 2023 and

10billion in 2057 (Worldmeters 2020; Roser et al. 2019).

With a large and growing population food supply necessarily has to increase. However,

nowadays there is a wealth of data that indicates that agriculture is the cause of numerous

environmental problems. It uses half of the planet habitable land and accounts for 70% of

water consumed worldwide. Moreover, it is the major pollutant of water and one of the

principals of land (Greentumble 2018; Ritchie & Roser 2019; OECD wy).

For these reasons, new methods of agriculture that focus on sustainability should be

studied and implemented. This thesis focuses on studying the sustainability of aquaponics,

a soilless method that proved to consume 90% less water and use six times less land than

conventional agriculture while raising fish (Shelley 2018). A system designed to be located

in Vaasa, Finland, is studied in this thesis.

2. Purpose

The aim of the thesis is to undertake a full Life Cycle Analysis of a designed aquaponic

system and evaluate its sustainability by comparing it with traditional agriculture and

aquaculture.

The objectives of the thesis are:

- Design an aquaponics taking as a base a prototype setup and scaling it up to fit in a

container.

- Evaluate the environmental impact of the whole aquaponic system using SimaPro.

- Compare the sustainability significance between the aquaponic system and

traditional agriculture and aquaculture.

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3. Research

The research consists of the definition of the theoretical starting points and the explanation

of the project’s theoretical background. It is fully based in previous studies, definitions and

normative.

3.1 Theoretical starting points

In this section, the keywords to understand the thesis are explained. Aquaponics, life cycle

assessment and impact categories and endpoint characterization factors are the introduced

concepts.

3.1.1 Aquaponics

Aquaponics is a method to grow fish and plants combining hydroponics and aquaculture

respectively. This hybrid takes advantage of the pros of each system and reduces their

drawbacks. In addition, it has been studied as a more sustainable technique to grow plants

than traditional agriculture (The Aquaponic Source 2020; Nelson Pade 2018).

Figure 1 represents simply how aquaponics work. Firstly, there is a tank where fish, that

can be for consuming or not, live. They are feed with fish food, and they excrete waste

mainly in the form of ammonia. At high concentrations, it can be toxic for both fish and

plants. Therefore, this wastewater goes through a mechanical filter, where bigger solids are

removed. After it, a biofilter of bacteria transforms ammonia into nitrite, and nitrite into

nitrate. This rich in nutrients water flows through the roots of the plants. They live soilless,

with a part of the roots in the water to absorb the nitrates, and the other part in the air, so

they can take oxygen from it. Clean water can now come back to the first tank, where the

fish will excrete again their waste (Barry 2019; Doityourself 2010).

Figure 1: Functioning of an aquaponic system (Alonso et al. 2019).

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3

Growing fish and plants in an almost closed loop solves many problems of raising them

separately. On the one hand, conventional agriculture requires fertilizers and huge amounts

of water. On the other hand, aquaculture has to face ammonia accumulation in water,

which can be toxic for the fish. Aquaponics uses this ammonia to feed the plants and just

10% of the water required in conventional agriculture (The Aquaponic Source 2020;

Rombaugh 2018).

3.1.2 Life Cycle Assessment

The Life Cycle Assessment (LCA) is a tool to evaluate the cradle-to-grave environmental

performance of a product or a service in all the phases of its life. It includes the obtainment

of the materials, the production, use, end-of-life treatment, recycling and the final disposal.

It intends to compare, not to give absolute results, so including other stages such as

packaging or transport, depends on each case. To do it, Finland is bound to implement the

European Standard ISO14040. This standard defines the use that can be made from the

LCA and the phases it should contain (Muralikrishna & Manickam 2017, 57-75; ISO-

Norm 2006; Curran 2008; Ashby 2012, 49-75).

According to ISO-Norm (2006), there are four steps to be followed when carrying out an

LCA. They can be found all throughout the thesis, where they are described. The first one

is the goal and scope definition that in the end should be accomplished (see Chapter 5.2).

Next, there is the inventory analysis, which describes the materials and energy flows in the

system (see Chapter 5.3). Then, in the impact assessment phase, the results in the impact

categories are detailed (see Chapter 6). Finally, in the interpretation step, a critical

discussion is carried out and the results and sensitivity analysis are presented (see Chapter

7).

3.1.3 Impact categories and endpoint characterization factors

Each one of the materials and energy flows in the system produce consequences to the

environment, named impact categories. In Chapter 6, the impacts produced by the

aquaponic system are quantified. To understand them, the impacts that are not self-

explicative like "land use" or "water consumption", are defined in this section.

Besides, each one of the impact categories has a unit associated depending on the damage

category they affect. Human health is calculated in Disability-Adjusted Life Year (DALY).

One DALY corresponds to a year of healthy life lost, so the results are the sum of the

DALYs of all the population caused by the product evaluated. If the damage category is

the resources, it is measured in USD2013, the dollars that these resources would cost in

2013. Finally, the ecosystems’ damage category uses the unit species.yr. It is a measure of

how many living species are expected to disappear per year as a consequence of the

product. The addition of all the environmental impacts which use the same unit results in

the endpoint characterization factors (WHO 2013; Vieira et al. 2016; PRé et al. 2016).

Global warming is the result of the accumulation of greenhouse gases in the Earth's

atmosphere. They retain the heat produced by the surface's radiation from going to space.

However, global warming does not just imply melting the glaciers and the rise of the sea

level, it is a cause of a generalized climate change with effects such as harder tropical

Page 11: Shipping Container Aquaponics: Life Cycle Assessment and

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storms, frequent wildfires and drought in some zones. Therefore, it affects all three damage

categories (NASA 2020).

Ozone in the stratosphere filters the ultraviolet radiation from the sun, protecting humans

from health problems such as skin cancer. However, if the ozone is near the ground, it may

cause respiratory problems. Hence, both stratospheric ozone depletion and ozone formation

are impacts that will be taken into account (EEA 2016; UK AIR 2010).

Eutrophication is the enrichment of the environment by nutrients, causing the increase of

algae and plants. These harm water quality, elevating pH and CO2 levels. These factors

finally cause fish death (National Ocean Service 2017; Chislock et al. 2013).

Terrestrial acidification is a process that occurs naturally by the colonization of rock

surfaces by algae and lichens. Carbon and nitrogen cycles generate acids, which are

involved in dissolving rock and soil minerals, so over the time pH decreases, meaning that

soils become more acidic. This process occurs naturally but is being accelerated by

agriculture practices. Plants decrease their magnesium and phosphorus concentration, and

as a consequence, there is a reduction in coverage, root growth and biomass. Moreover,

germination and regeneration become unsuccessful and acid-tolerant species invade the

soil (Robson 1989; Azevedo et al. 2013, 10-15).

Ionizing radiation is a type of energy that can cause health problems. Humans are exposed

to it by natural sources in water, air and soil. Depending on the effective dose, measured

with variables like the type of radiation, the potential for causing harm varies (WHO

2016).

The ecotoxicity of a chemical is the potential adverse effect that it can cause to the

environment (National Research Council 2014). In the LCA made in Chapter 6, it is

measured in the terrestrial, freshwater and marine environments.

3.2 Theoretical background

The framework where the study is made is the previous aquaponic system designed and

built in the university; the preceding literature made by other authors, which gives an idea

of the expected results of the thesis; and the location, basic to define the requirements of

the system.

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3.2.1 Testing setup

This thesis is a development of the initial work undertaken by European Project Semester

students. Their four-month project culminated in the fabrication of an experimental

aquaponics setup. This was installed in the basement of Novia University of Applied

Sciences (see Figure 2). Citations used in this thesis fully acknowledge this previous work,

where materials were not published they have been referenced as “unpublished work”.

Figure 2: Testing setup (Alonso et al. 2020).

3.2.2 Previous studies

The previous LCA made on aquaponic systems have shown a significant improvement in

environmental performance if compared with conventional agriculture methods.

Traditional agriculture accelerates eutrophication because of the use of fertilizers; while in

aquaculture residues from fish are released in the water. From the combination of these

techniques appears aquaponics, solving both problems by the recirculation of the water.

Moreover, as the water is used in a loop, water requirements are less than 10% of what

would be used in traditional agriculture. Land use is also lower than in typical agriculture

due to the possibility of stacking hydroponic systems in vertical (The Aquaponic Source

2019; McGraw 2017; Delp w.y).

Therefore, as it would be expected, aquaponics proved to be more sustainable in many

impact categories. It excelled in reducing water consumption and eutrophication, so these

are the main expected results from this study (Ghamkhar et al. 2019; Cohen et al. 2018).

3.2.3 Characteristics of Vaasa

The system is designed to be operating in Vaasa, a city on the west coast of Finland. It is

relevant knowing the characteristics of the place where it is going to be working to choose

the more appropriate species and components for the system. Besides, the waste scenario

of the aquaponics will depend on the residues policies taken in Vaasa.

Also, the energy source of the electricity that the system is using is an important factor to

take into account. Results can vary depending on if it comes from fossil fuels or renewable

Page 13: Shipping Container Aquaponics: Life Cycle Assessment and

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energy sources. The study of where the electricity mix in Finland’s grid comes is crucial,

but as there are libraries that provide this data to the program that calculates the

environmental impacts, SimaPro, it is not necessary to do previous research.

Climate

The climate in the place where the aquaponic system is located is important to know if

artificial lighting and heating are required. Besides, depending on if the climate is cold or

warm, different species will be more or less adequate for the system. Firstly, the daylight

and sunshine daily hours depending on the month are shown in Figure 3. In June, there is

daylight 20h/day, in contrast with the less than 5h/day in December. This means that

species that can adapt to this lighting should be chosen or artificial light has to be provided.

Figure 3: Daylight and sunshine hours by month in Vaasa (Weather Atlas 2017).

Next, temperatures determine which species are more adequate for the aquaponics.

Additionally, the heating requirements depend on the exterior temperatures. In Vaasa,

temperatures vary from -12ºC in winter to 20º in summer (see Figure 3). Therefore, cold

climate species are preferable over warm ones and a heating system will be required.

Figure 4: Maximum and minimum temperatures by month in Vaasa (Weather Atlas 2017).

To sum up, Vaasa has a cold climate and lighting is highly dependent on the season. This

means that good insulation and heating should be provided. Besides, species that can adapt

to cold climates and big changes in light should be chosen, or artificial light has to be

provided.

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Waste scenario

One of the main phases of the Life Cycle of a product is waste treatment and the disposal.

For this reason, it is important to consider a realistic waste scenario that would have the

aquaponic system. Finland´s Government has made a National Waste Plan for 2023 with

the objective of move on a circular economy (Laaksonen et al. 2018, 11-16).

This can be seen in Figure 5, wherein 2017, just around 1% of the municipal solid waste

ended up in landfills. There is a growing inclination to recover energy and, to a lesser

extent, to recover material. In any case, statistics show that the disposal in a landfill is

being replaced by waste recovery. For the current study, the proportions of 41% of material

recovery, 58% of energy recovered and 1% of landfill sending will be considered

(Statistics Finland 2019).

Figure 5: Amount of municipal waste by treatment from 2013 to 2017 (Statistics Finland 2019).

Waste-to-Energy is the name that receives the recovery of energy from residues. It is

known that one of the plants from Finland, the Riikinvoima Ekovoimalaitos Waste-to-

Energy plant, generates 180GWh of district heat and 90GWh of electricity from

145000tones of municipal solid waste (Making Future 2017). With the respective

conversions, this means 1241Wh/kg of district heat and 620Wh/kg of electricity generated

from residues.

It is useful to know the evolution of the waste scenarios in past years and the intentions in a

close future because the aquaponics will be used for 15 years. The actual ones do not seem

too optimistic if taking a look into the evolution. Consequently, the actual ones will be

considered for the study.

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4. Design of the aquaponic system

In this section, each one of the elements that compose the aquaponic system is explained.

The design is made component by component, so depending on the previous ones selected;

the characteristics of the following ones are restricted.

4.1 Container

Having an aquaponic system in a container has some benefits: it is portable, easy to

customize and stackable. Some companies have already installed aquaponic systems in

containers such as Growtainer (2012), who personalizes and sends them around the world

or GrowUp Urban Farms (2018) that produced 20,000kg of salads and 4,000kg of fish in a

year. Even EDEN ISS (2019) has a container with plants in Antarctica to test if it would be

a good solution to grow food safely in the space. For the current study, it is advantageous

that it was tested before, so the aquaponic system will be designed to fit in a 20ft shipping

container.

The external dimensions of a standard 1C freight container are 6058x2438x2438mm

(length x width x height). They are commonly known as 20ft containers because this is

their nominal length. The minimal internal dimensions of this type of container are

5867x2330x2197mm (ISO-Norm 2013). Shipping containers can be made of many

materials; however, they are mainly made of steel (Wankhede 2019).

The system will be installed in a cold climate, so insulation is a crucial part to have the

desired temperature inside the container. The team that worked in building the aquaponics

setup in the university modeled a 20ft container and the insulation required to install it in

any part of Finland. From the study, it was deduced that 150mm of EPS in every wall with

40mm of radiant barrier (air) would produce effective insulation. The floor insulation

should not include radiant barrier as there is no radiation through the ground. In addition, it

is recommended to paint the outside with anti-radiant paint (unpublished work).

4.2 Hydroponic systems

An aquaponic system is the symbiosis of hydroponics and aquaculture. Hydroponics is

defined as a technique for growing plants without soil, with the roots with direct contact to

a nutrient-rich solution (Fullbloom Hydroponics 2011). A first draft about the number of

plants and the occupied space will determine the size and characteristics of the other

components.

Before distributing the system, the objective of building it should be defined. Most of the

companies specialized in aquaponics do not obtain their benefits just from selling products

related to aquaponics (components of the system, fish or plants). Most of them offer

courses to teach the method (Aquaponics Iberia w.y; Nelson Pade 2018). Besides,

Mauriceri et al. (2018) reported the benefits of teaching a wide variety of subjects using an

aquaponic system. Thereby, the design of the study will be distributed in a way that allows

having visitors.

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There are many types of aquaponic systems, but just the ones that have been tested in the

university will be included in the design because they performed well. These are deep

water culture (DWC) and nutrient film technique (NFT) towers, which will be explained in

this chapter. A draft of the design can be seen in Figure 6.

Figure 6: Draft design of the aquaponic system (in mm).

4.2.1 Deep water culture (DWC)

Deep water culture technique is considered the easiest and the most effective method of

hydroponics. Plants are held in a recipient with an amount of water sufficient to maintain

the density of nutrients almost constant. The plants have their roots sunk in the water

continuously oxygenated. This can be accomplished with airstones connected to an air

pump as shown in Figure 7 (Green and Vibrant 2019).

Figure 7: Deep Water Culture System (Green and Vibrant 2019).

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Many companies used this method for their hydroponic systems. In Figure 8, the interior of

the EDEN ISS container is shown. A similar design, also with steel shelves and four rows

will be made.

Figure 8: EDEN ISS interior of the container (Haeckels 2019).

The final DWC design of the hydroponic systems consists of three rafts as the one shown

in Figure 9 displayed in one long side of the container. Plants are held up by plant pots in

each one of the holes. As there are 4 levels, 12 rafts are required. They are made out of

expanded polystyrene so they float in the water. Each one of them has the place to grow 24

plants, so in total there will be growing 540 plants.

Figure 9: Design of the raft.

4.2.2 Nutrient film technique (NFT) towers

The second system is made of PVC pipes with some holes and inorganic foam with a very

low density inside. The plants crop up from the holes and are hold by the foam (see Figure

9). Water is dropped from above the tube and it goes through the roots, which absorb the

nutrients from it. Then, water is collected by funnels to a pipe. The setup built in the

university performed well until plants died because of low maintenance. Water got

evaporated from the biofilter tank, and consequently, there was no water available for the

plants. As a result, they dried in a few hours unlike the plants in the DWC, which had

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enough reservoirs of water in their tanks to survive. Although the bad experience with this

design, with more maintenance, many authors used it successfully (Alonso et al. 2020;

Freightfarms 2019).

Figure 10: Subjection of the plants and interior of the vertical towers (Verburgh et al. 2020 & unpublished work).

In essence, it is a type of nutrient film technique (NFT), a method of hydroponics used

basically for leafy plants. It is the most popular technique, probably because it uses little

space and a wide variety of designs can be selected. A thin layer of nutrient solution flows

through the roots of the plants, which absorb the nutrients while being oxygenated

(Hydroenv 2014).

The design can be seen in Figure 11 and it will consist of 15 towers with 9 spots for lettuce

each of them, letting 135 spots for plants. The space in the left, painted with pink stripes, is

left for the tanks, and the one in blue, for the controlling system.

Figure 11: Design of the towers.

4.3 Plants

Almost any type of plant can grow well in an aquaponic system. However, some are more

suitable than others. For example, tomatoes, leafy greens and peppers are good options

(Mattson 2016).

Combining different species in the same aquaponics is an attractive option because when

incorporating the adequate plants in the same system, they benefit from each other. An

example is when a plant that produces chemicals that repeal certain insects is planted next

to one that is highly affected by pests (Somerville et al. 2014, 119). However, as matching

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the required parameters of different species can be challenging, for the investigated system

just one type of plant will be chosen.

Next, the type of the plants should be decided. Fruity plants, leafy greens and roots are the

main types of plants that are used in aquaponics. They should grow in the hydroponic

systems selected, but the towers are not suitable for heavy plants, so leafy ones are

preferred. Besides, fruit plants require a higher nutrient concentration and more light

(Somerville et al. 2014, 92). Therefore, the aquaponic system from the current study will

just include one type of vegetable which does not bear fruit.

In the setup from the university, there were six types of plants, all of them leafy ones:

lettuce, arugula, spinach, Swiss chard, parsley and basil. All of them had similar behavior.

However, from these, in 2018 the most imported product was lettuce, representing 2.19%

of all the value trade of vegetable imports in Finland (OEC 2020). Moreover, lettuce is one

of the most used and studied plants for aquaponics and hydroponics (Cohen et al. 2018;

Freightfarms 2019).

4.3.1 Lettuce characteristics and parameters

Lettuce (Lactuca sativa) is a type of leafy plant, which in general has a low nutrient and

light demand (Somerville et al. 2014, 92; Encyclopædia Britannica 2008). This means that

with the same amount of fish, more lettuce than other types of plants can grow in the

aquaponics. In Table 1 some parameters that lettuce needs to grow are presented.

Table 1: Required parameters for lettuce.

Parameter Value Reference

Tolerable water temperature 15-22ºC (Somerville et al. 2014, 171)

Air temperature during the day 17-28ºC (Somerville et al. 2014, 171)

Air temperature during the night 3-12ºC (Somerville et al. 2014, 171)

Optimal p.H levels 5.8-6.2 (Somerville et al. 2014, 171)

Tolerable p.H levels 5.8-7.0 (Somerville et al. 2014, 171)

Dissolved Oxygen 7-8ppm (Mattson 2016)

Calcium 90ppm (Mattson 2016)

Maximum plant densities 20-25 heads/m2

(Somerville et al. 2014, 171)

Growth time 24-32 days (Somerville et al. 2014, 171)

Many elements are required by the plants, but just calcium is shown in Table 1 because a

common problem when growing lettuce in a hydroponic system is tip burn of the inner

leaves, caused by calcium deficiency. The young leaves develop necrosis, and as a

consequence, they grow deformed and with hooked tips. The plants uptake and distribute

Ca by active xylem transpiration, but when humidity is too high, leaves cannot transpire.

Therefore, this can be avoided by providing more airflow to the plants (Somerville et al.

2014, 88; HortiDaily 2019).

4.4 Fish

Once the plants of the system have been decided, the types of fish that can be selected are

restricted. The water in the aquaponics is the same for the plants and the fish and

consequently, the required parameters for the fish and the plants must be similar. Other

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conditionings to choose the type are the priority of local fish and the suitability for living in

aquaponics.

Nile tilapia (Oreochromis niloticus) is the most common fish in aquaponics, but it cannot

be fished near Vaasa. In Finland, there are more than 60 fish species that can be usually

found in its waters, and even more, if considering occasional visitors (Encyclopædia

Britannica 2011; FishinginFinland 2014). Some of them such as perch and trout have been

used for many authors in their aquaponics systems (Leaffin 2019c; Brooke 2019b; Weldon

2019; Brooke 2018), so much information about how to grow them in aquaponics is

available. Both fish species are possible candidates for the system of the study, but there is

a priority in choosing perch because of the experience in the testing setup in the university.

4.4.1 Yellow perch characteristics and parameters

Yellow perch (Perca fluviatilis) is a warm type of coolwater fish (Leaffin 2019c), but it is

also categorized as freshwater fish (Brooke 2019b; Encyclopædia Britannica 2020). It can

live in a wide range of temperatures and pH levels (see the values in Table 2), making it

suitable for aquaponics. Besides, it quickly adapts to the available food source (Brooke

2019b).

Table 2: Required parameters for yellow perch.

Parameter Value Reference

Tolerable range of temperatures 18,3ºC-26,7ºC (Brooke 2019b)

Optimal range of temperatures 20ºC-25,6ºC (Brooke 2019b)

pH levels 6,5-9,2 (Weldon 2019)

Dissolved Oxygen 3,5mg/L to saturation (Weldon 2019)

Ammonia <0,0125mg/L (Weldon 2019)

Nitrite <1,0mg/L (Weldon 2019)

Calcium 10-160ppm (Weldon 2019)

However, before choosing any fish, it is necessary to assure that it can live with lettuce. If

comparing the parameters that are important for both plants and fish, which can be found

in Table 1 and Table 2, Figure 12 is obtained.

Tolerable temperatures 14 15 16 17 18 19 20 21 22 23 24 25 26 27 ºC

Lettuce

Perch

Tolerable pH 5.0 5.2 5.4 5.6 5.8 6.0 6.2 6.4 6.6 6.8 7.0 7.2 7.4 7.6…

Lettuce

Perch

Figure 12: Lettuce and perch parameters comparison.

As it can be seen in Figure 12, lettuce and perch share an interval of temperatures and

tolerable pH where they both can live. Consequently, they can grow together in an

aquaponic system.

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4.4.2 Fish ratios

When designing an aquaponic system, all the parts are dependent on the rest of the system.

For example, depending on the surface available for plants, the amount of fish is restricted.

In this chapter, the required biomass of fish will be calculated following the order that can

be seen in Figure 13.

Figure 13: Order of calculations to follow when sizing an aquaponic system.

The water should remain in a range of nutrients concentration that allows plants to have

enough nutrients without letting them accumulate in the water. In other words, it should be

a balance between the production of nutrients and the uptake of them (Leaffin 2019a;

Tidwell et al. 2012, 364-366). For this reason, the amount of plants restricts the amount of

fish.

In general, to feed 1m2 of plant growing area, 100g of fish food should be daily input.

However, lettuce has a low nutrient demand, and just 40-50g of fish food a day will be

enough to fulfill their needs. From the fish food, 80% is used by the fish, and the rest is

excreted as waste (Leaffin 2019a; Tidwell et al. 2012, 364-366; Somerville et al. 2014,

124).

In total, there are 17,4m2 of plants in the system. Consequently, between 696g and 870g of

fish food should be daily input in the system (see Eq.1). Fish eat 1-2% of their body weight

a day depending on their growth stage: young fish eat more than older (Somerville et al.

2014, 124; Aller-aqua 2018). Consequently, 34.8kg – 87.9kg of fish are required in the

aquaponics (see Eq. 2.1 and Eq. 2.2).

The maximum stocking density is 20kg of fish per 1000L of water. However, high

densities are the first cause of fish stress and it complicates the control of the aquaponics

(Somerville et al. 2014, 125). To avoid that, just 15kg per 1000L will be grown in the fish

tank.

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The common plants used in aquaponic systems can grow in a wide range of nutrient

density (Tidwell et al. 2012, 364-366). For this reason and because of the variability of fish

food that perch eat, the amount of fish that can be input in the system varies from 34.8kg to

87.9kg. An amount of fish in the middle of the range will be selected to avoid

complications. If considering 60kg of fish, 4000L of water is required.

The number of fish obtainable from the weight depends on their size when input in the

system. The information related to their sizes, weight and ages can be found in Table 3.

Table 3: Length and weight of perch according to their age.

Time Length Weight Reference

Perch fingerlings 1-2 months 2,5-3,5cm - (Weldon 2019; The Fish Site

2007)

Harvest size 12-18 months 10-15cm 0,23-0,34kg (Brooke 2019b)

Final grow 10 years 25-30cm 1kg (Brooke 2019b)

To obtain fish all the year-round, yellow perch in different growth stages will be contained

in the fish tank. Every three months, fish in the harvest size should be collected and the

same amount of fingerlings should be added (see Table 4). From it, it can be deduced that

the medium fish sizes 0.23kg. As there are 60kg of fish, there are approximately 260 fish.

If every three weeks one fifth can be harvested, 52 fish can be collected.

Table 4: Average lengths and weights of the perch in the system according to their age.

Age in the

beginning

After 3

months

Age in the harvest

time of the oldest

Average lengths Average weights

2 months 5 months 3.5cm 0.09kg

5 months 8 months 7cm 0.18kg

8 months 11 months 10cm 0.23kg

11 months 14 months 12.5cm 0.29kg

14 months 17 months 15cm 0.34kg

Average 9.6cm 0.23kg

To sum up, perch will be the fish of the system. It is a suitable fish to grow in aquaponics

and it can be fished in Finland. For the number of plants that need to be feed, 260 fish

weighting together 60kg is required. To avoid stressing them, they will live in a 4000L fish

tank.

4.5 Fish food

One of the reasons yellow perch are a good selection for aquaponics is because it is easy to

adapt them to alternative diets. Some authors recommend alimenting them with the same

feed than trout or salmon. It can be explained because both fish share many characteristics.

They are between freshwater and cold water carnivore fish (Brown et al. 1996, 171-174;

Brooke 2019b; Leaffin 2019b; Encyclopaedia Britannica 2020). This fact is an

advantageous coincidence for the project because fish food labels do not display the exact

amount of each ingredient, but in the SimaPro libraries, there is already trout feeding as a

material that can be directly inputted to the LCA.

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It is recommended to have fish in mixed grow rates to harvest regularly (Leaffin 2019a).

However, for every fish length, it is preferred choosing a different size of fish food (Aller-

aqua 2018). As in the tank of the current study, there are fish from mixed lengths; the

middle size of fish food should be chosen. From the test in the university, it was proven

that perch prefer flakes than granulated fish food. They are thin enough for the small fish,

so they can eat them. As a consequence, trout feeding in the shape of a flake will be used.

4.6 Fish tank

The fish tank is a crucial element in the aquaponic system. To grow healthy fish and plants,

the right one should be chosen (Green and Vibrant 2019).

On the one hand, any fish tank that can support the weight of the water will work.

Therefore, if taking into account the objective of building the aquaponic system, having a

transparent tank, the fish would be seen and it would be more visual for the students

coming to visit it. Also, it is recommended having cylindrical tanks to make it easier

cleaning it. In rectangular tanks, fish waste gets stuck in the corners and water does not

flow as well as in tanks with round walls (FAO 2015; Green and Vibrant 2019).

Although glass is the most common material in aquariums, if the objective is having a

cylindrical tank, it will be more expensive than rectangular ones. Besides, due to its

dimensions, it will be heavy and it is not easy finding the desired shape and size. Another

option that is gaining popularity is acrylic fish tanks. This material is stronger and lighter

than glass, but over the time it loses quality due to the yellowing and the easy apparition of

scratches, and it is also expensive (Fish Geeks 2016; Glasscages 2007; Glasscages w.y).

As reported in Chapter 4.4.2, the required volume to fit the needed amount of fish is

4000L. If just taking into account the best option for the fish, big tanks help the

maintenance of a stable system (Green and Vibrant 2019). However, big transparent tanks

are expensive and there is less availability. For further studies, the economic part and the

opportunity of finding the tank should be considered, but for the current one, just the best

tank for the fish will be chosen. As a result, a bit oversized acrylic and cylindrical tank

with a diameter of 1.6m, the height of 2.2m and wide of 20mm will be selected.

A complete acrylic tank would be too expensive and difficult to manufacture. Therefore,

80% of the tank will be made of polyethylene (PE) and the rest of acrylic. This amount

should be enough for the students to see the fish.

4.7 Filtration

In the aquaponics systems, two types of filtration are required. Mechanical filtration is

responsible for accumulating and removing waste. Whereas biological filtration dissolves

some particles and makes easier for the plants to absorb the nutrients (Somerville et al.

2014, 44-48).

4.7.1 Mechanical filtration

Mechanical filtration is one of the most delicate parts of the design. Many issues can affect

both the health of the living species and clogging the system if a proper waste removal is

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not accomplished. For these reasons, some mechanical filtration methods will be discussed

in this chapter (Somerville et al. 2014, 44-48).

In small-scale aquaponics, clarifiers can remove 60% of the solids and as a consequence,

they are the most recommended method. Clarifiers are tanks designed in a way that makes

the water flow slower. When this happens, particulates suspending in water accumulate in

the bottom, where they can be removed, so clarifiers perform as settlers. To collect the

waste, they are connected to the bottom of the fish tank by a pipe that sucks the wastewater

(Somerville et al. 2014, 44-48; Brooke 2019a).

There are two main types of clarifiers: the ones that make the water flow slower by making

it swirl and the ones with baffles inside. Solids should be periodically removed by

connecting a pipe with a minimum diameter of 30cm to the bottom. To assure the

collection of waste, the tank uses to have a conical shaped bottom (Somerville et al. 2014,

44-48; Brooke 2019a).

A general rule recommends using a tank six times smaller than the fish tank, but the

optimal design will depend on the rest of the system (Somerville et al. 2014, 44-48). This

approximation is enough for the design, so a cylindrical container with a conical bottom

with a capacity of 667L will be used in the system of the study. This can be achieved with

the design shown in Figure 14. Many fish tanks are made of polyethylene (Green and

Vibrant 2019), so this is the material that will be considered for it.

Figure 14: Conical clarifier with baffles (Somerville et al. 2014, 45)

4.7.2 Biological filtration

Mechanical filtration dissolves the biggest waste particles that are remaining in the water.

However, some of them dissolve or are too small to be captured in this way and biological

filtration is required. Besides, the nitrite and the ammonia in the water should be converted

into nitrate also by biological filtration to simplify the plants the uptake of nutrients. This is

why another tank where the majority of the bacteria live should be integrated into the

system (Somerville et al. 2014, 44-48).

The characteristics that this tank should accomplish to have healthy growing bacteria is

having a large surface area and oxygenated water. To increase the surface area, bioballs are

commonly used. They are usually made of plastic and have a considerable surface area if

taking into account their small volume (Somerville et al. 2014, 44-48). They can be seen in

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Figure 15. In the testing setup, the research group improved the designs available in the

shops and on the Internet and 3D printed them. They were made of ABS and there will be

200 of them in the system. The tank size uniquely depends on the available space in the

container (see the sizes in Appendix 1 Data collection).

Figure 15: Design of the bioball used in the testing setup (unpublished work).

4.8 Pump

The sizing of the pump is a key issue for the normal operation of the aquaponic system.

Having the wrong size of the pump can result in giving excessive or insufficient nutrients

to the living parts of the system. The objective of having the correct pump is maximizing

the effectiveness of aquaponics (Castelo 2018).

There are two main types of pumps: the submersible and the inline ones. The first type

should be situated in a water tank. They are usually used in small commercial aquaponics

that do not need to more than 1200GPH (gallons per hour). The inline pumps are used for

big aquaponic systems with more than 50 towers (Castelo 2018; Storey 2016). Therefore,

as the aquaponics has to fit in a container, and there are few towers and rafts, the optimal

pump is the submersible.

Knowing the type of pump, the required size can be calculated. The first step is

determining the GPH or LPH (liters per hour). Generally, all the water should circulate

through the system every two hours (Castelo 2018; Storey 2016). As the system from the

current study contains approximately 5240L of water, the pump should pump at least

2620L of water each hour, which is the same than 692GPH.

The next step is measuring the heat height. This is the distance between the water level in

the grow beds or the towers and the level in the sump tank where the pump is situated

(Castelo 2018; Storey 2016). In the case of the current study, the maximum distance that

can be between the levels is 1.8m, or which is the same, 5.9 feet.

Finally, each manufacturer provides some charts that in one axis contain the GPH and the

other one, the heat height. With this information, the pump related to the line that matches

the operation of the system should be chosen (Castelo 2018; Storey 2016). In Figure 16,

the heat height and flow rate chart from the pumps from ActiveAQUA, a company that

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produces components for aquaponics, can be seen. The red cross shows the conditions that

the pump should reach according to the studied system.

Figure 16: Heat height and flow rate chart from ActiveAQUA pumps (ActiveAQUA 2016).

From the characteristics that should meet the pump, the selected one is AAPW1000,

available in Amazon. Its weight is 8.62 pounds, or 3.9 kg, of mixed materials. As the

amount of each one of the materials is not easy to know, and the total weight is low

compared to other components, just the power consumption in its operation will be

considered. It can supply up to 92W (Amazon w.y).

4.9 Water

Aquaponics is a system where the water is conserved; it flows through the different

components and loses are almost irrelevant if comparing with other methods of agriculture.

However, as they are produced every day, they become significant, and water should be

introduced periodically. The principal reasons for water escaping the system are

evaporation and leaks, but also wastewater from the clarifier can have an impact (Baptista

2014; Somerville et al. 2014, 44-48).

Plants use the water in the evapotranspiration and they retain it in their tissues. Besides,

water is directly evaporated from the tanks. In general, depending on the system

characteristics, an aquaponics has a daily water loss of 1-3% (Baptista 2014; Somerville et

al. 2014, 29)

The system of the study should be built in a closed container and in a cold climate, factors

that reduce the evaporated water. For these reasons, the lower value from the normal water

use ranges will be considered. This means that 1% of the water in the system should be

introduced every day. As in the system of the study, there are approximately 5240L of

water, everyday 52.4L of water should be input.

Despite this, for the study, no water outputs will be considered. This is because any of the

processes where water is output from the system has a significant ecological impact.

Firstly, when the water is evaporated, it will return to the Earth’s surface as rainwater.

Secondly, water is absorbed by the plants, which is already considered in the LCA. Finally,

water and solids are removed from the bottom of the clarifier. These contain high densities

of nutrients and can be used as precious fertilizers (Somerville et al. 2014, 44-48).

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In an improved system, it would be interesting to include a rainwater collection system.

Rainwater is perfect for aquaponics because it usually has neutral pH and low salinity.

Besides, it will improve the sustainability of the system and reduce costs (Somerville et al.

2014, 30).

4.10 Lights

Both plants and fish require light to live. Coldwater fish need light between 8h/day and

12h/day. As it has to be tested to know the optimal amount of light hours, the upper value

in the range is going to be chosen to not obtain too optimistic results. A slow transition

from darkness to light and the other way round replicating natural cycles will help the fish

to do not stress (Waithaka 2019).

Though, plants need more specific requirements. Firstly, they demand a defined

combination of colors in each stage of its life for an optimal grown. Next, according to the

plant species, the intensity required is different. In the case of lettuce, low intensity is

enough. Finally, different species require different periods of light. Some sources assure

that lettuce requires from 14h to 18h/day of light (D’Anna 2019; Richards 2019), while

some others explain that 10h to 12h/day is enough (Allman 2013; NoSoilSolutions 2018).

Again, testing will say, but for the moment, 14/day is supposed.

4.11 Controlling system

The correct operation of the system requires having the ambient conditions prepared for

the living species. A controlling system connected to several sensors and actuators should

be installed to achieve it. For the LCA, the consumption of a Raspberry Pi and an Arduino

board is considered (Alonso et al. 2019).

4.12 Final design

To sum up, the final design consists of a 4000L fish tank and two different types of

hydroponics: DWC, with 12tanks, and the NFT, with 19 towers. The used plants are

lettuce, and the fish, perch. It also includes complementary components such as the

required for the filtration, for connecting the components and the controlling system.

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5. Life Cycle Assessment of the aquaponic system

In this chapter, some of the phases of the LCA of the previously designed aquaponic

system are made following the ISO 14040 standards. It includes the explanation of the

software and the method used, SimaPro and ReCIPe 2016 (H), the goal and scope

definition and the inventory analysis.

5.1 SimaPro and ReCiPe 2016 (H)

The software used in this study is SimaPro, the computer program leading LCA for 30

years in more than 80 countries. There is a variety of licenses for companies and education

institutions in a range of prices from 350€ to 7000€. It follows ISO 14044 and 14040

standards and contains libraries with information that reduces the amount of data that the

user has to introduce. It has been used in many LCA investigations by researchers and

several companies used it to evaluate their products and services (PRé 2016).

Many methods can be used for the LCA, but for this investigation, ReCiPe 2016 Endpoint

(H) method is chosen as the most suitable. It analyzes 21 impact categories, more than

many of the methods, and some authors used it before for their LCA on aquaponics, so it

simplifies comparisons with literature (PRé 2019; PRé 2016).

The user can select any of the three variants of the ReCiPe Endpoint method according to

the objective of the study. For a time horizon of 20 years, the Individualist perspective (I)

should be used. On the contrary, for considering long-term impacts that may not be fully

established and pessimistic future socio-economic developments, the Egalitarian

perspective (E) is the best variant. For the studies where the most frequent policy

principles are taken into account, the Hierarchy perspective (H) should be selected

(ReCiPe manual). For this study, the default variant (H) is used (PRé 2019).

5.2 Goal and scope definition

The LCA is carried on to evaluate the environmental impacts of the whole aquaponic

system and compare it with the ones caused by conventional agriculture and aquaculture.

This is made using the same functional unit as the other studies, in this case, 1kg of fish.

It includes the phases from the extraction of the material to its disposal, including the

transport of the components from the factory that produces them to Vaasa. However, it is

excluded the transport of the materials from its extraction location to the factory. The

packaging is neither considered. All the contemplated components are explained in their

respective sections in Chapter 4 and Appendix 1 Data collection, with their forming

materials and amounts.

Breeding and seeding are out of the boundaries of the system, so perch fingerlings and

lettuce sprouts should be bought. With perch avoiding breeding is easy because they

require temperatures to about 7ºC for at least a month and having mature fish, which is not

going to happen in the system (Brooke 2019b).

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5.3 Inventory analysis

Collection of data is a crucial part of the project. The LCA results will fully depend on the

considerations and assumptions taken on this part. It is supposed that the system will last

15 years, and after it, it will be disposed in the typical waste scenario in Finland (see

Chapter 3.2.3). Long operation times will reduce the impacts made by equipment

comparing with the other assembly groups (see them in Chapter 6.1). As can be seen in

Figure 17, the inputs are the components and the operation of the system, and the outputs,

the fish and the lettuce. In the sections of each one of the components, the taken data are

explained (see Chapter 4), but the exact information introduced in Simapro is explained in

Table 6, Table 7, Table 8 and Table 9; found in Appendix 1.

Figure 17: Inputs and outputs of the system.

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6. Results

After introducing the data to SimaPro, the impacts of the described aquaponic system are

calculated using the method ReCipe 2016 (H). This chapter contains an explanation of the

process tree and the assembly groups, the impact assessment and the comparison of the

results from other aquaponics, traditional agriculture and aquaculture.

6.1 Process tree and assembly groups

Some authors in the literature had divided the components and processes into groups to

identify the main causes of each impact or damage categories. The same groups have been

used in the current study to easily compare the results. These are equipment, heating,

electricity and fish food (Ghamkhar et al. 2020). These assembly groups exclude tap water,

perch fingerlings and lettuce sprouts. However, their impacts can be neglected because the

defined groups produce more than 93% of the impact in all categories of characterization.

Table 1 is the process tree with all the processes and components of the aquaponic system.

Each one of them has assigned a color that classifies them to their corresponding group.

Note that the waste scenario is included in the equipment group.

Figure 18: Process tree diagram of the life cycle of the aquaponic system and assembly groups.

Figure 18 is a simplification of the real process tree. Each one of the components data used

for the calculations includes many processes, materials, emissions, etc. such as the

manufacturing processes and the transport (see Appendix 1 for the exact data).

6.2 Impact assessment

The impact assessment phase is made to understand the significance and importance of the

potential environmental impacts in the whole life cycle of the designed aquaponic system

(ISO-Norm 2006). There will be explained and illustrated the characterization and the

damage assessment of the aquaponics. Moreover, the results will be compared with those

obtained by other authors in similar studies.

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6.2.1 Characterization

The characterization is an essential step according to ISO. To know the relative

contribution of a substance to an impact category, they are multiplied by a characterization

factor (PRé 2019, 1-2). By this way, knowing the substances that appear in the life cycle of

the aquaponic system, it is known their contribution to a specific impact category (See

Chapter 3.1.3 for the explanation of the impact categories). Figure 19 is the

characterization graph by assembly groups, created from Table 11, and this one from Table

10, directly outputted from SimaPro. Both tables are found in the Appendix 2. Lines in

Figure 19 correspond to the assembly groups, and the relative percentage of contribution of

each one of them is shown in the net.

Where: Global warming, Human health (GW,HH); Global warming, Terrestrial ecosystems (GW,TE); Global warming,

Freshwater ecosystems (GW, FE); Stratospheric ozone depletion (SOD); Ionizing radiation (IR); Ozone formation,

Human health (OF,HH); Fine particulate matter formation (FPMF); Ozone formation, Terrestrial Ecosystems (OF,TE);

Terrestrial acidification (TA); Freshwater eutrophication (FE); Terrestrial ecotoxicity (TE); Freshwater ecotoxicity (FE);

Marine ecotoxicity (ME); Human carcinogenic toxicity (HCT); Human non-carcinogenic toxicity (HNCT); Land use

(LU); Mineral resource scarcity (MRS); Fossil resource scarcity (FRS); Water consumption, Human health (WC,HH);

Water consumption, Terrestrial ecosystem (WC,TE); Water consumption, Aquatic ecosystems (WC,AE).

Figure 19: Characterization by assembly groups.

From Figure 19, it highlights that in many categories, the element that produces a higher

impact is equipment, followed by electricity. Whereas, the impact produced by heating and

perch food is minimal if compared with the other two.

Ghamkhar et al. (2020) made a similar LCA of a cold-weather aquaponic system in

Stevens Point, Wisconsin. However, the results they obtained are different. In their study,

the parameters with a major significance were heat and electricity. It cannot be explained

by weather differences, because as shown in Figure 20, in general, Vaasa has lower

temperatures than Stevens Point. Therefore, the difference in results must be explained in

another way. There are many types of aquaponics, so it is an option that Ghamkhar et al.

0

20

40

60

80

100 GW,HH

GW,TE

GW,FE

SOD

IR

OF,HH

FPMF

OF,TE

TA

FE TE FE

ME

HCT

HNCT

LU

MRS

FRS

WC,HH

WC,TE

WC,AE

Heating (%) Perch food (%) Equipment(%) Electricity(%)

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(2020) made the study on an aquaponic system that requires much less equipment. The

current study includes a whole shipping container, the transport of the components from

the company where they are manufactured to Vaasa and the waste scenario for each of

them. Besides, it is considered that the shipping container is well insulated with EPS,

which lowers the energy consumption invested in heating. As it is not known the

considerations that were used for the other study, no conclusions can be made.

Figure 20: Temperature average comparison of Vaasa and Stevens Point (Weatherbase w.y)

6.2.2 Damage assessment

In the damage assessment step, the impact category indicators expressed with the same unit

are added in a common damage category. ReCiPe 2016 (H) uses three endpoint

characterization factors to classify the impact category indicators: human health,

ecosystems and resources (See the explanation of the impact category indicators in Chapter

3.1.3) (PRé 2019, 1-26). Figure 21 is the damage assessment by assembly groups obtained

from Table 12 in the Appendix 2.

Figure 21: Damage assessment by assembly groups.

It can be seen that although in the characterization (Figure 19) energy is not the main

contributor in most of the impacts when just looking at the damage assessment graph

0%

10%

20%

30%

40%

50%

60%

70%

80%

90%

100%

Human health Ecosystems Resources

Electricity

Equipment

Perch food

Heating

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(Figure 21), it is consolidated as the most damaging parameter. This is because in Figure

19 the impact categories where energy has a higher percentage, the environmental impacts

are high (it has elevated values of DALY, species.yr or USD 2013). Per contra, in the

impacts equipment has a higher percentage, the environmental impacts are low (see the

exact data in Appendix 2).

Thus, in all the categories the factor that has a higher significance is electricity, followed

by the equipment. Closely next to it there is the heating and with a lower impact, perch

food.

Similar results were found by Maucieri et al. (2017) that built a micro aquaponic system

for educational proposes with recovered materials. He found that electricity is the most

significant impact on the aquaponic system, while water consumption was the lowest. It is

remarkable the low effect that produced the equipment in their study, which produced less

than 16% of the impact in all categories. This can be explained by the use of recovered

materials, which demonstrated to be a major sustainable choice.

6.3 Impact assessment ignoring the effects of the shipping container

If just taking a look in the equipment assembly group, it highlights that the sipping

container is the main contributor in 15 of the 21 impact categories. This is calculated

without ignoring the waste scenario because shipping container contributes to it too. See

Figure 22 for the relative contribution of equipment, without taking into account the waste

scenario, to all the studied impact categories with and without shipping container. It is

created from Table 13 in the Appendix 2.

Figure 22: Relative contribution to the impact categories of the equipment with and without the shipping container

ignoring the effects of the waste scenario (%).

Knowing the importance of the shipping container, it is interesting to evaluate if the results

would be similar without it. Both characterization and damage assessment will be

calculated again. Figure 22 is the characterization graph calculated with these new

conditions (see the complete name of the impacts in Figure 19). Now electricity is the main

contributor, gaining in 6 more categories than Figure 19 where it produced the major

significance in 9 of 21 categories. It is obtained from the data from Table 14 in the

Appendix 2.

0

20

40

60

80

100

Without shipping container With shipping container

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Figure 23: Characterization graph by assembly group without including the container.

If looking at the new damage assessment in Figure 23, made using the data from Table 15

in the Appendix 2, important reflections can be made. If the container is excluded from the

calculations, the percentage of damage in the ecosystems and human health caused by

heating now gets closer to the caused by equipment (compare with Figure 19). Even more

interesting is looking at the resources damage column, where both perch food and heating

overcome equipment damage. With this consideration, electricity gets largely considered

as the main contributor causing more than 60% of damage to each category.

Figure 24: Damage assessment graph by assembly group without including the container.

From now, the container impacts will be omitted to understand which ones of the results

are caused by the system, and not by the housing. Later on, in Chapter 7, it will be

developed in greater depth.

0

20

40

60

80

100 GW,HH

GW,TE

GW,FE

SOD

IR

OF,HH

FPMF

OF,TE

TA

FE TE FE

ME

HCT

HNCT

LU

MRS

FRS

WC,HH

WC,TE

WC,AE

Equipment Heating Electricity Perch food

0%

10%

20%

30%

40%

50%

60%

70%

80%

90%

100%

Human health Ecosystems Resources

Electricity

Equipment

Perch food

Heating

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6.4 Comparison with conventional agriculture and aquaculture

Aquaponic systems have been reported as a greener technique than conventional

agriculture and aquaculture. In this section, this is going to be proved by using the

available data obtained from other studies and the results from this report.

6.4.1 Comparison with conventional agriculture

The aquaponic system of the study uses 292.1 tones of water to produce 82,712 clumps of

lettuce with an average weight of 100g. This means, in 15 years the system will be able to

produce 8,271kg of lettuce with water use of 35L/kg of lettuce.

A study made in Yuma, Arizona in the USA by Lages et al. (2015) accounted for

250±25L/kg of lettuce grown by conventional agriculture. The investigated aquaponics

system used between 12.7% and 15.6% of the water required for conventional agriculture,

a value close to the 10% published in the literature (Winkler 2008; The Aquaponic Source

2020). In Figure 25 can be visually seen the difference.

Figure 25: Water requirements comparison between conventional agriculture and aquaponics.

However, it is known than the requirements of electricity are higher in aquaponics than in

other techniques, even more, if it is located in a cold climate (Hhamkhar et al. 2020). In 15

years of operation, the aquaponics from the investigation used 318,645kWh. As there were

produced 8,271kg of lettuce, this means that it used 38.53kWh/kg of lettuce. Conventional

agriculture uses 1,100±75kJ/kg of lettuce, while aquaponics used 138,692kJ/kg of lettuce;

around 130 times the electricity required with the conventional method (see Figure 26).

0

50

100

150

200

250

Conventional agriculture Aquaponics

L/kg

of

lett

uce

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Figure 26: Energy requirements comparison between conventional agriculture and aquaponics.

This value is about 50% higher than what it was published in the literature for hydroponics.

It would be expected to have a closer result because the electronic equipment necessary is

almost the same. This time, the difference can be explained by heating requirements

caused by the difference in temperatures (see Figure 27). In addition, the considered

hydroponics system is installed in a greenhouse. This means that probably there is a natural

source of light and energy requirements invested in light are much lower than in the

aquaponics closed in the container, where lighting accounts for more than 90% of

electricity usage (Lages et al. 2015).

Figure 27: Temperature average comparison between Vaasa and Yuma (Weatherbase w.y)

These results can make one think that aquaponics is not as sustainable as it is reported in

the literature, so in the next section it is going to be evaluated.

0

20000

40000

60000

80000

100000

120000

140000

Conventional agriculture Aquaponics

kJ/k

g o

f le

ttu

ce

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6.4.2 Comparison with conventional agriculture combined with aquaculture

In this study, it has been discussed a lot about agriculture, but considering the fish as a co-

product of the system is essential to understand its effectiveness. In all the time of

operation, the system will produce 1,101.6kg of perch. Cohen et al. (2018) made an LCA

comparing conventional agriculture and two types of aquaculture with aquaponic. Their

results are compared with the ones obtained in this project in Table 5. Additionally, a

fourth column with the results of the current study if wind energy is used instead of the

electricity mix in Finland’s grid is used. In light blue, it is indicated the less contributor

system in each impact, while as darker is the blue, worst is the system performing in that

category.

Table 5: Each system performance to the impact categories (Modified from Cohen et al. (2018)).

Impacts

Unit

Pond-based Aquaculture, Conventional agriculture

Lake-based Aquaculture, Conventional Agriculture

Aquaponics Cohen et al. (2018)

Aquaponics using electricity mix from Finland's grid

Aquaponics using energy from the wind

Global warming, Human health DALY 3,75E-01 3,50E-01 7,64E-02 2,97E-01 1,01E-01

Global warming, Terrestrial ecosystems species.yr 1,08E-03 1,06E+03 2,30E-04 8,98E-04 3,04E-04

Global warming, Freshwater ecosystems species.yr 2,94E-08 2,88E-08 6,30E-09 2,45E-08 8,32E-09

Stratospheric ozone depletion DALY 4,25E-05 2,73E-04 3,25E-04 1,41E-04 7,93E-05

Ionizing radiation DALY 6,26E-05 3,80E-05 4,61E-04 1,16E-03 5,42E-06

Ozone formation, Human health DALY 4,39E-04 4,30E-04 2,00E-04 4,85E-04 1,61E-04

Fine particulate matter formation DALY 3,10E-01 3,06E-01 4,35E-04 1,65E-01 1,18E-02

Ozone formation, Terrestrial ecosystems species.yr 6,44E-05 6,29E-05 2,90E-05 6,93E-05 2,30E-05

Terrestrial acidification species.yr 3,98E-04 3,94E-04 1,40E-04 1,38E-04 2,57E-05

Freshwater eutrophication species.yr 1,07E-04 1,09E-04 1,55E-05 1,43E-05 4,91E-06

Terrestrial ecotoxicity species.yr 4,71E-06 4,54E-06 1,19E-06 1,40E-05 8,27E-07

Freshwater ecotoxicity species.yr 2,75E-06 2,67E-06 5,33E-07 3,43E-05 3,21E-05

Marine ecotoxicity species.yr 5,73E-07 5,57E-07 9,64E-08 6,82E-06 6,34E-06

Human carcinogenic toxicity DALY 2,51E-02 2,46E-02 1,19E-02 3,87E-02 1,82E-02

Human non-carcinogenic toxicity DALY 3,96E-02 3,88E-02 1,48E-02 2,13E-01 1,86E-01

Land use species.yr 1,32E-03 1,32E-03 1,23E-03 8,68E-05 3,34E-05

Mineral resource scarcity USD2013 5,57E+01 5,25E+01 1,21E+02 1,26E+02 1,80E+01

Fossil resource scarcity USD2013 1,32E+04 1,24E+04 6,49E+03 1,03E+04 2,33E+03

Water consumption, Human health DALY 7,50E-02 6,74E-01 1,72E-02 3,89E+00 6,35E-01

Water consumption, Terrestrial ecosystem species.yr 4,56E-04 4,10E-03 1,05E-04 2,36E-02 3,86E-03

Water consumption, Aquatic ecosystems species.yr 2,04E-08 1,83E-07 4,68E-09 1,06E-06 1,73E-07

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From Table 5 it highlights the low impacts of the aquaponics from Cohen et al. (2018)

study and from the one from this study when a renewable source of energy is used.

However, the results have to be interpreted carefully.

Firstly, because the aquaponics investigated in the present study produces approximately

1kg of fish of each 8kg of lettuce, while the Cohen et al. (2018) systems produce 1kg of

fish for each 5kg of lettuce. As the functional unit is 1ton of fish, the designed aquaponics

is generating a higher production of lettuce with those impacts. If the functional unit would

be 5kg of lettuce, then the impacts would be reduced.

Secondly, it should be taken into account that Cohen et al. (2018) are producing the lettuce

in a greenhouse in Switzerland, a warmer country than Finland. It would be more

interesting to compare which is the most sustainable method to obtain fresh products in

Vaasa, but the study does not include the transport. As a consequence, it cannot be

compared systems in cold climates with systems in warm climates, because the first one

will generally have bigger impacts.

Although all this, it is enough clear that the aquaponic system which uses wind energy is

the greener of the options. It showed a similar performance than Cohen et al. (2018)

aquaponics, but this one did not include the transport, so it cannot be known if when the

products arrive at Vaasa will pollute more or less than the ones obtained in the local

aquaponics. As expected, all aquaponic systems had a better environmental performance in

eutrophication, land use and water consumption. In the following chapter, all the issues

found in this one will be discussed to obtain conclusions.

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7. Discussion

In this chapter, it will be discussed if the initial objectives are achieved, the validity of the

results in comparison with other published work, the limitations of the study and the

consistencies and inconsistencies of it.

The first objective of this thesis was to design an aquaponic system taking as a base the

prototype setup described in Chapter 3.2.1 and scaling it up to fit in a shipping container.

By describing the components that should be in the system, sizing them and deciding its

amount, this objective was met. The decisions made in the design are directly related with

the impacts of the system and the credibility of the results when comparing with other

studies.

The next two objectives are to evaluate the environmental impact of the designed

aquaponics using SimaPro and compare it with the sustainability significance of traditional

agriculture and aquaculture. Both objectives were met in Chapter 6 by using the obtained

results to create graphs and tables and using the data to compare it with other articles.

Unlike other articles, this one studied the sustainability of housing the aquaponic system in

a shipping container, a common practice in aquaponics companies. However, including it

in the study made mask the real impacts from aquaponics, so it had to be excluded. To

minimize the ecological impact of it, a reused shipping container can be restored for

accommodating an aquaponics. Another option is to provide housing for the system by

building a structure from less polluting or from recycled materials. Though, it should

provide similar or more insulation than the shipping container to not increase the

ecological cost in other categories.

This investigation coincided with other published work in the improvement of aquaponics

over conventional agriculture and aquaculture in the eutrophication, land use and water

consumption impacts. It highlights that electricity is the main contributor of the system,

consuming 50% more energy than hydroponic systems studied.

The reason for this high value of electricity consumption can be found in its usage,

contrasting with other studies in using 90% of it for the lighting. As lettuce requires little

light, it should be tested if the LEDs are consuming too much power. In that case, the

system would be consuming less than it was calculated. Also, it was considered that lettuce

is a long-day plant, but literature reported contradictory results about it. Ideally, it should

be tested the efficiency of the system with daylight between 8h and 14h, and then decide if

14h/day was too much for the optimal grown of lettuce. Besides, if another type of housing

that takes advantage of the daylight in summer is chosen, much energy can be saved. For

instance, LumiGrow (2018) used sensors that detect when the sunlight is intense to

automatically close the lights.

Moreover, energy proved to be the major contributor when the electricity came from the

mix in the grid, but not when it came from the wind, where the whole aquaponics showed

the best environmental performance. Some authors investigated the consequences of using

renewable energy sources instead of fossil fuels and reported that their use can reduce

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environmental impacts (Forchino et al. 2017). Consequently, deeper studies on the optimal

source of the electricity according to the location should be made.

The low values of heating impacts comparing with other cold weather aquaponic systems

can be explained by the good insulation on the system, but also by overrating the heat that

the selected heater can produce. In the testing setup experiments and models made in the

university, the material and the thickness of the insulation was decided, but there was

omitted the use of the heater. The next step is testing the performance of various heaters to

choose the best one and to concrete how much power they require.

Less important is that there have been found more efficient methods to produce the tanks

than thermoforming, such as rotational molding, being PE the ideal material for this

technique. It is relatively new compared to thermoforming, but more energy efficient.

Nevertheless, there should be a company that produces them this way near Vaasa, and this

is not the responsibility of the aquaponics builders (BPF 2015; euRECIPE 2006, 41-48).

To sum up, the objectives were achieved successfully, and in general, the results appeared

to be similar than LCA made on aquaponics. Further studies on environmental housing

structures, the light requirements of lettuce and energy sources should be made to improve

the sustainability performance of the designed system. Besides, more reliable results would

be obtained if this study was compared with more articles.

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8. Conclusions

Aquaponics can be part of the solution to the climate crisis. It demonstrated to improve the

environmental performance of agriculture and aquaculture before and this study is one

more proof of it. This bachelor thesis presents an LCA made on an aquaponic system

located in a cold-weather region in 21 impact categories. The results revealed that an

optimization on equipment, focusing on the housing, and on the electricity will derive to a

significant reduction on the environmental impacts.

The housing for the aquaponics was at first chosen to be a shipping container because

many companies succeed using it. However, the environmental impacts caused by it were

bigger than the ones produced by all the other equipment components, masking their

significance. When the impacts made by the shipping container were ignored, the results

are shown to be similar to the ones from other studies, especially concerning

eutrophication, land use and the use of water.

Despite this, electricity usage in the designed aquaponics was much higher than in the ones

from literature, being the LED lights the major consumers. To reduce their consumption, it

is recommended to experiment with the intensity and the daily hours they are turned on.

Summarizing, housing and electricity are the most sensitive parameters in the aquaponic

system. They are points where further investigation should focus on to optimize the

sustainability of the technique. The ball is now in the court of the entrepreneurs and

farming sector, that will have to decide if this technology is mature and with sufficient

potential to invest in.

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10. Appendices

Appendix 1 Data collection Table 6: Components

Component Material Size Density Weight Amount References

Fish tank

Acrylic (PMMA) (20%)

W=20mm Interior measures (cylinder): D=1.60m H=2.2m Total material: 0.27m3

Total capacity: 4.42m3

1.15g/mL

1150kg/m3 62.1kg

1

(Polymerdatabase 2018)

Polyethylene (PE) – High density (HDPE) (80%)

0.91-0.97g/cm3 Common value: 0.94g/cm3 940kg/m3

248.4kg

(Omnexus 2017b)

Total (100%)

- 310.5kg -

Clarifier

Polyethylene (PE) – High density (HDPE)

W=20mm Interior measures (cylinder): D=0.75m H=1.4m Interior measures (cone): D=0.75m H=0.5m Total material: 0.08m3

Total capacity: 0.692m3

0.91-0.97g/cm3 Common value: 0.94g/cm3 940kg/m3

75.2kg

1

(Omnexus 2017b)

Biofilter tank

Polyethylene (PE) – High density (HDPE)

W=10mm Interior measures (cylinder): D=0.35m H=1.5m Total material: 0.017m3 Total capacity: 0.128m3

0.91-0.97g/cm3 Common value: 0.94g/cm3 940kg/m3

16.0kg

1

(Omnexus 2017b)

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46

Bioballs

Acrylonitrile Butadiene Styrene (ABS)

Measures (approx. cylinder): D=74mm H=70mm Total: 14.5cm3

1.020-1.210g/cm3 Average value: 1.115g/cm3 1115kg/m3

1.617·10-5kg/bioball 3.23kg

200

(Omnexus 2018)

Shipping container

Steel - - 30480kg

1

(ISO-Norm 2013; Wankhede 2019; Workshop 2012; BigSteelBoxTV 2011)

Wood (oak) – used for the floor

8.86m3

600-900kg/m3 Average value: 750kg/m3

6645kg

Total - - 37125kg

Insulation

Expanded Polystyrene (EPS)

4.521m3

11-32kg/m3 Common value: 32kg/m3

144.67kg

-

( Australian Urethane & Styrene w.y)

Pipe towers

Rigid Polyvinyl Chloride (PVC)

Dexterior=110mm W=5mm L=1257mm

1.3-1.45g/cm3 Average value: 1.375g/cm3 1375kg/m3

2.89kg/tower 43.37kg

15

(Omnexus 2017a)

Pipes (for connecting components)

Rigid Polyvinyl Chloride (PVC)

Dexterior=50mm W=4mm L=27m (aprox.)

1.3-1.45g/cm3 Average value: 1.375g/cm3 1375kg/m3

21.46kg

-

(Omnexus 2017a)

Towers’ funnels

Acrylonitrile Butadiene Styrene (ABS)

33.57cm3

1.020-1.210g/cm3 Average value: 1.115g/cm3 1115kg/m3

0.561kg

15

(Omnexus 2018)

Filter foam

Polyurethane

50x300x400mm=6·106mm3 0.006m3/filter foam sheet Total: 0.27m3

30kg/m3

8.1kg

3filters/tower x 15 towers

= 45

(Modulor w.y)

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47

Plant pots

Acrylonitrile Butadiene Styrene (ABS)

Dexterior=70mm W=3mm H=70mm 70% of the surface is a hole (see Figure _) 1.616·10-5m3

1.020-1.210g/cm3 Average value: 1.115g/cm3 1115kg/m3

0.018kg/pot 9.73kg

540

(Omnexus 2018)

Fish food (trout feed) – Includes transportation of the ingredients to the factory

Fishmeal (47%) - - 1791.17kg - Library: LCA Food DK (SimaPro)

Wheat (14%) - - 533.54kg -

Rape seed oil (20%) - - 762.2kg -

Soy meal (18%) - - 685.98kg -

Chemicals (1%) - - 38.11kg -

Total (100%)

-

-

696g/day x 365days x 15 years 3811kg

-

Perch fingerlings

Trout*

-

-

0.07kg/fingerling 226.8kg

54perch x 4 times/year x

15years = 3240perch

-

Lettuce sprouts

Lettuce

-

4g/lettuce sprout

4g/lettuce sprout 330kg

423lettuce

sprouts/28days x 365days x 15years =

82712

-

Shelves

Steel

1320x750x1950mm

-

54.43kg/shelf 163.29kg

3

Data adapted from (Amazon w.y-c)

Rafts

Expanded Polystyrene (EPS)

1320x750x40mm 0.0396m3

11-32kg/m3 Common value: 32kg/m3

15.2kg

12

( Australian Urethane & Styrene w.y)

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*There is no data available for perch. Therefore, as trout is the most similar fish with available data, it is selected.

Table 7: Manufacturing processes.

Process Components Power consumption and emissions

Hours Energy consumption

Reference

3D printing

Bioballs 50W

5h x 200bioballs 50kWh (3dstartpoint 2016) Towers funnels 4h30min x 15funnels 3.38kWh

Plant pots 3h x 540pots 81kWh

Thermoforming Fish tank The library offers a long list of emissions and energy input from different sources

-

-

SimaPro libraries Clarifier

Biofilter tank

Corrugating Shipping container

The libraries offer a long list of emissions, inputs and outputs

-

-

(BigSteelBoxTV 2011) SimaPro libraries Arc welding

Painting

DWC tanks

Polypropylene (PP)

1320x750x200mm w=1mm 0.001813m3

0.970g/cm3 970kg/m3

1.76kg/tank 21.1kg

12

(Omnexus 2018)

Water

Water

Initial input: 5240L Variable input: 52.4L/day

1kg/L

5240kg initial + 52.4kg/day x 365days/year x 15years 292130kg

-

-

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49

Table 8: Operation process.

Process Components Power consumption and emissions

Hours Energy consumption

Reference

Pumping Pump 92W 24h/day x 365days/year x 15 years 12,088.8kWh (Amazon w.y-a)

Heating Heater

750W 24h/day x 365days/year x 15 years x 0.9* 88,695kWh (Amazon w.y-b)

Lighting LEDs 100W x 42 stripes of LEDS = 4200W**

365days/year x 15 years x (14h/day x 39/42 + 12h/day x 3/42)

318,645kWh (Lumeri w.y)

Controlling the system

Raspberry Pi ~10W 24h/day x 365days/year x 15 years 1,314kWh Real data from the setup in the university Arduino ~24W 3,153.6kWh

Oxygenate DWC Air pump 25W 24h/day x 365days/year x 15 years x 2 units

6,570kWh (Wish w.y)

* Taking into account the cold climate in Vaasa (see Chapter 3.2.3) it is considered that it will be working 90% of the time.

**39 stripes for pants and 3 stripes for fish

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50

The infrastructure of Finland for the transport of goods is well developed. It has more than commercial 50 ports, including Vaasa’s port (Santander

2018). For this reason, as it can be seen in Table 9, all the components arrive to Finland by ship.

Table 9: Transport.

Component Company Origin place Method of transport

Distance Comments Reference

Fish tank, Clarifier and Biofilter

Tanks Direkt Rotterdam, Netherlands

Ship 114.5km - (Tanks Direkt 2020) Truck 2098.5km

Shipping container

Scandic Container

Vaasa, Finland

-

-

As Vaasa is a port city, it is considered that it is not going to be difficult to find a used container. The company from the reference offers shipping containers in Finland.

(Scandic Container 2017)

Fish food Skretting Stavanger, Norway Ship 95.8km - (Skretting 2018)

Truck 1441.2km

Perch fingerlings

-

Vaasa, Finland -

-

Perch is a native fish from Finland (see Chapter 4.4). It would be necessary to contact with local fishermen to assure the supply of fingerlings

Chapter 4.4

Insulation (EPS boards) EWI Store Kingston, United Kingdom

Ship 114.5km - (EWI Store 2020)

Truck 2540.5km

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Appendix 2 Results from SimaPro

Table 10: Characterization by components and processes.

Impact category Unit Waste scenario Fish tank Clarifier

Biofilter tank Bioballs

Shipping container

Insulation (EPS)

Pipe towers

Pipes for connecting components

Towers funnels Filter foam

Global warming, Human health DALY 9,78E-02 8,72E-04 1,35E-04 2,87E-05 2,49E-05 7,74E-02 6,29E-04 1,41E-04 6,96E-05 2,80E-06 2,47E-05

Global warming, Terrestrial ecosystems species.yr 2,95E-04 2,63E-06 4,07E-07 8,66E-08 7,52E-08 2,33E-04 1,90E-06 4,24E-07 2,10E-07 8,45E-09 7,46E-08

Global warming, Freshwater ecosystems species.yr 8,06E-09 7,19E-11 1,11E-11 2,37E-12 2,05E-12 6,38E-09 5,18E-11 1,16E-11 5,73E-12 2,31E-13 2,04E-12

Stratospheric ozone depletion DALY 5,76E-05 7,46E-08 1,81E-08 3,84E-09 2,03E-09 5,57E-06 4,10E-08 1,73E-09 8,55E-10 1,37E-10 2,05E-08

Ionizing radiation DALY 1,60E-06 2,26E-07 5,46E-08 1,16E-08 8,60E-09 2,20E-06 8,52E-08 0,00E+00 0,00E+00 5,81E-10 1,75E-09

Ozone formation, Human health DALY 6,85E-05 4,58E-06 9,45E-07 2,01E-07 3,29E-08 1,21E-04 4,61E-06 2,37E-07 1,17E-07 5,68E-09 3,66E-08

Fine particulate matter formation DALY 4,55E-03 9,44E-04 1,66E-04 3,52E-05 1,43E-05 4,65E-02 2,73E-04 7,66E-05 3,79E-05 1,59E-06 5,43E-06

Ozone formation, Terrestrial ecosystems species.yr 9,75E-06 6,52E-07 1,35E-07 2,86E-08 4,72E-09 1,77E-05 6,60E-07 3,36E-08 1,66E-08 8,10E-10 5,43E-09

Terrestrial acidification species.yr 8,75E-06 1,24E-06 2,14E-07 4,55E-08 2,05E-08 6,12E-05 6,33E-07 1,09E-07 5,41E-08 2,40E-09 8,85E-09

Freshwater eutrophication species.yr 4,84E-06 6,76E-08 1,44E-08 3,06E-09 5,79E-13 1,80E-06 7,17E-10 8,41E-10 4,16E-10 3,91E-14 1,19E-10

Terrestrial ecotoxicity species.yr 7,64E-07 5,87E-09 1,42E-09 3,02E-10 4,60E-11 3,69E-07 7,53E-10 2,80E-10 1,39E-10 3,11E-12 8,12E-11

Freshwater ecotoxicity species.yr 3,66E-05 7,69E-09 1,86E-09 3,96E-10 9,82E-13 2,71E-08 1,34E-09 1,33E-11 6,56E-12 6,80E-14 2,49E-12

Marine ecotoxicity species.yr 7,23E-06 1,53E-09 3,71E-10 7,90E-11 3,91E-13 9,95E-09 3,05E-10 2,73E-12 1,35E-12 2,64E-14 2,42E-12

Human carcinogenic toxicity DALY 2,02E-02 3,72E-05 8,79E-06 1,87E-06 1,89E-08 1,75E-03 1,63E-06 2,19E-04 1,08E-04 1,37E-09 1,30E-07

Human non-carcinogenic toxicity DALY 2,12E-01 3,98E-05 9,64E-06 2,05E-06 6,31E-08 1,16E-03 2,94E-05 1,08E-07 5,32E-08 4,26E-09 1,35E-06

Land use species.yr 2,58E-06 1,06E-07 2,46E-08 5,23E-09 9,69E-10 2,66E-04 0,00E+00 0,00E+00 0,00E+00 1,46E-10 1,51E-08

Mineral resource scarcity USD2013 9,23E+00 1,70E-01 4,06E-02 8,63E-03 9,24E-04 1,93E+02 2,94E-02 7,49E-03 3,70E-03 9,29E-05 1,13E-02

Fossil resource scarcity USD2013 7,08E+02 1,34E+02 3,18E+01 6,76E+00 1,99E+00 1,99E+03 1,46E+02 2,02E+01 1,00E+01 2,50E-01 5,10E+00

Water consumption, Human health DALY 1,32E-03 3,29E-04 7,78E-05 1,66E-05 2,37E-01 2,34E-04 4,39E-04 7,72E-06 3,82E-06 1,60E-02 3,46E-05

Water consumption, Terrestrial ecosystem species.yr 8,05E-06 2,00E-06 4,73E-07 1,01E-07 1,44E-03 1,42E-06 2,67E-06 4,69E-08 2,32E-08 9,74E-05 2,10E-07

Water consumption, Aquatic ecosystems species.yr 3,60E-10 8,94E-11 2,12E-11 4,50E-12 6,45E-08 6,37E-11 1,20E-10 2,10E-12 1,04E-12 4,36E-09 9,41E-12

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Impact category Unit Plant pots Perch food Trout Lettuce Shelves Rafts DWC tanks Water Electricity Heating

Global warming, Human health DALY 5,50E-05 9,11E-03 3,80E-04 1,18E-03 1,52E-04 3,43E-05 7,59E-05 4,49E-05 1,26E-01 3,34E-02

Global warming, Terrestrial ecosystems species.yr 1,66E-07 2,75E-05 1,15E-06 3,57E-06 4,58E-07 1,04E-07 2,29E-07 1,35E-07 3,81E-04 1,01E-04

Global warming, Freshwater ecosystems species.yr 4,54E-12 7,51E-10 3,13E-11 9,75E-11 1,25E-11 2,83E-12 6,26E-12 3,70E-12 1,04E-08 2,76E-09

Stratospheric ozone depletion DALY 3,28E-09 2,64E-05 1,49E-06 4,07E-07 7,33E-10 3,35E-09 2,49E-09 1,08E-08 4,85E-05 1,28E-05

Ionizing radiation DALY 1,39E-08 1,65E-06 1,41E-07 1,24E-07 0,00E+00 8,94E-09 0,00E+00 7,05E-07 8,66E-04 2,29E-04

Ozone formation, Human health DALY 9,86E-08 8,70E-05 1,64E-06 1,34E-06 6,96E-07 5,20E-08 1,69E-07 9,62E-08 2,06E-04 5,46E-05

Fine particulate matter formation DALY 3,14E-05 3,54E-03 7,76E-05 1,10E-03 1,66E-04 1,13E-05 8,60E-05 2,56E-05 9,12E-02 2,41E-02

Ozone formation, Terrestrial ecosystems species.yr 1,41E-08 1,25E-05 2,39E-07 1,96E-07 9,87E-08 7,79E-09 2,46E-08 1,40E-08 2,95E-05 7,81E-06

Terrestrial acidification species.yr 4,65E-08 1,23E-05 3,27E-07 1,08E-06 2,66E-07 1,66E-08 1,12E-07 2,73E-08 5,48E-05 1,45E-05

Freshwater eutrophication species.yr 9,38E-13 4,30E-08 2,93E-07 2,53E-07 5,00E-11 7,53E-11 0,00E+00 1,53E-09 1,31E-06 3,46E-07

Terrestrial ecotoxicity species.yr 7,45E-11 2,79E-08 2,49E-09 1,94E-08 6,17E-09 6,80E-11 5,23E-11 1,82E-09 2,58E-06 6,82E-07

Freshwater ecotoxicity species.yr 1,61E-12 1,93E-08 6,78E-11 9,77E-09 4,41E-11 1,19E-11 8,66E-11 9,17E-10 3,68E-07 9,75E-08

Marine ecotoxicity species.yr 6,33E-13 4,06E-09 2,61E-11 2,11E-09 3,07E-11 5,81E-12 1,72E-11 2,01E-10 8,91E-08 2,36E-08

Human carcinogenic toxicity DALY 3,18E-08 1,42E-05 5,33E-07 9,49E-05 4,88E-06 9,76E-08 7,74E-08 2,05E-05 3,99E-03 1,06E-03

Human non-carcinogenic toxicity DALY 1,02E-07 3,16E-04 1,66E-06 1,10E-04 8,60E-06 1,01E-06 1,16E-06 9,51E-06 5,97E-03 1,58E-03

Land use species.yr 2,62E-09 2,92E-05 1,63E-06 5,07E-07 7,96E-08 0,00E+00 0,00E+00 4,48E-08 4,08E-05 1,08E-05

Mineral resource scarcity USD2013 1,90E-03 3,01E-01 2,48E-02 1,31E-01 2,22E+00 3,08E-03 0,00E+00 1,18E-01 3,67E+01 9,71E+00

Fossil resource scarcity USD2013 4,73E+00 1,11E+03 3,66E+01 3,96E+01 1,14E+01 1,04E+01 9,04E+00 2,38E+00 5,80E+03 1,53E+03

Water consumption, Human health DALY 3,84E-01 2,05E-03 1,69E-04 1,33E-05 4,28E-07 4,62E-05 0,00E+00 3,37E-03 2,41E+00 6,38E-01

Water consumption, Terrestrial ecosystem species.yr 2,33E-03 1,25E-05 1,03E-06 8,11E-08 2,61E-09 2,81E-07 0,00E+00 2,05E-05 1,47E-02 3,88E-03

Water consumption, Aquatic ecosystems species.yr 1,04E-07 5,59E-10 4,59E-11 3,63E-12 1,17E-13 1,26E-11 0,00E+00 9,16E-10 6,56E-07 1,74E-07

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Table 11: Characterization by assembly groups.

Impact category Unit Heating Perch food Equipment Electricity Total Heating (%) Perch food (%) Equipment(%) Electricity(%)

Global warming, Human health DALY 3,34E-02 9,11E-03 1,77E-01 1,26E-01 3,46E-01 9,65724062 2,63033785 51,2150242 36,4973973

Global warming, Terrestrial ecosystems species.yr 1,01E-04 2,75E-05 5,35E-04 3,81E-04 1,04E-03 9,65836375 2,62936679 51,2106256 36,5016439

Global warming, Freshwater ecosystems species.yr 2,76E-09 7,51E-10 1,46E-08 1,04E-08 2,85E-08 9,65680663 2,6299242 51,2175116 36,4957576

Stratospheric ozone depletion DALY 1,28E-05 2,64E-05 6,33E-05 4,85E-05 1,51E-04 8,49415873 17,4707191 41,9334647 32,1016574

Ionizing radiation DALY 2,29E-04 1,65E-06 4,21E-06 8,66E-04 1,10E-03 20,8126017 0,15021275 0,38262562 78,6545599

Ozone formation, Human health DALY 5,46E-05 8,70E-05 2,01E-04 2,06E-04 5,49E-04 9,94013786 15,8481692 36,6455837 37,5661092

Fine particulate matter formation DALY 2,41E-02 3,54E-03 5,29E-02 9,12E-02 1,72E-01 14,0448602 2,06264288 30,8134579 53,079039

Ozone formation, Terrestrial ecosystems species.yr 7,81E-06 1,25E-05 2,91E-05 2,95E-05 7,89E-05 9,89716003 15,8062136 36,8929387 37,4036877

Terrestrial acidification species.yr 1,45E-05 1,23E-05 7,27E-05 5,48E-05 1,54E-04 9,40151231 7,95435614 47,1135471 35,5305844

Freshwater eutrophication species.yr 3,46E-07 4,30E-08 6,73E-06 1,31E-06 8,43E-06 4,11026493 0,5104856 79,8457384 15,5335111

Terrestrial ecotoxicity species.yr 6,82E-07 2,79E-08 1,15E-06 2,58E-06 4,44E-06 15,380036 0,6277729 25,8668152 58,1253759

Freshwater ecotoxicity species.yr 9,75E-08 1,93E-08 3,67E-05 3,68E-07 3,72E-05 0,2622741 0,05196893 98,6945591 0,99119785

Marine ecotoxicity species.yr 2,36E-08 4,06E-09 7,24E-06 8,91E-08 7,36E-06 0,32036246 0,05513167 98,413778 1,21072792

Human carcinogenic toxicity DALY 1,06E-03 1,42E-05 2,24E-02 3,99E-03 2,74E-02 3,85204076 0,05185687 81,5381717 14,5579307

Human non-carcinogenic toxicity DALY 1,58E-03 3,16E-04 2,13E-01 5,97E-03 2,21E-01 0,71577853 0,14304127 96,4360877 2,70509253

Land use species.yr 1,08E-05 2,92E-05 2,69E-04 4,08E-05 3,50E-04 3,09052028 8,35654873 76,873062 11,679869

Mineral resource scarcity USD2013 9,71E+00 3,01E-01 2,04E+02 3,67E+01 2,51E+02 3,87086427 0,1200739 81,3801335 14,6289283

Fossil resource scarcity USD2013 1,53E+03 1,11E+03 3,09E+03 5,80E+03 1,15E+04 13,3009783 9,64496537 26,7862811 50,2677752

Water consumption, Human health DALY 6,38E-01 2,05E-03 6,39E-01 2,41E+00 3,69E+00 17,2889233 0,05559027 17,3170309 65,3384555

Water consumption, Terrestrial ecosystem species.yr 3,88E-03 1,25E-05 3,89E-03 1,47E-02 2,25E-02 17,2889238 0,05559027 17,3170311 65,3384548

Water consumption, Aquatic ecosystems species.yr 1,74E-07 5,59E-10 1,74E-07 6,56E-07 1,00E-06 17,2889232 0,05559027 17,3170313 65,3384552

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Table 12: Damage assessment by assembly group.

Damages Unit Heating Perch food Equipment Electricity Total Heating Perch food Equipment Electricity

Human health DALY 6,99E-01 1,52E-02 1,11E+00 2,64E+00 4,46E+00 15,6685376 0,33965114 24,777056 59,2147553

Ecosystems species.yr 4,02E-03 9,40E-05 4,85E-03 1,52E-02 2,41E-02 16,6421462 0,38940844 20,074264 62,8941813

Resources USD2013 1,54E+03 1,11E+03 3,29E+03 5,84E+03 1,18E+04 13,1002184 9,44218768 27,9485426 49,5090513

Table 13: Characterization comparison with and without shipping container.

Impact category Unit Total (including) Total (excluding) Difference Difference (%)

Global warming, Human health DALY 1,77E-01 1,00E-01 0,07736301 43,6171348

Global warming, Terrestrial ecosystems species.yr 5,35E-04 3,02E-04 0,00023342 43,6188715

Global warming, Freshwater ecosystems species.yr 1,46E-08 8,24E-09 6,3772E-09 43,618287

Stratospheric ozone depletion DALY 6,33E-05 5,78E-05 5,5739E-06 8,80192373

Ionizing radiation DALY 4,21E-06 2,01E-06 2,2012E-06 52,2672611

Ozone formation, Human health DALY 2,01E-04 8,02E-05 0,00012101 60,1295854

Fine particulate matter formation DALY 5,29E-02 6,40E-03 0,04654643 87,9091129

Ozone formation, Terrestrial ecosystems species.yr 2,91E-05 1,14E-05 1,7688E-05 60,75211

Terrestrial acidification species.yr 7,27E-05 1,15E-05 6,1156E-05 84,1506085

Freshwater eutrophication species.yr 6,73E-06 4,93E-06 1,7975E-06 26,7112382

Terrestrial ecotoxicity species.yr 1,15E-06 7,79E-07 3,6877E-07 32,1342784

Freshwater ecotoxicity species.yr 3,67E-05 3,67E-05 2,7089E-08 0,07385275

Marine ecotoxicity species.yr 7,24E-06 7,23E-06 9,9486E-09 0,13733314

Human carcinogenic toxicity DALY 2,24E-02 2,06E-02 0,00175214 7,83872306

Human non-carcinogenic toxicity DALY 2,13E-01 2,12E-01 0,00116008 0,54499397

Land use species.yr 2,69E-04 2,81E-06 0,00026594 98,9534873

Mineral resource scarcity USD2013 2,04E+02 1,17E+01 192,52147 94,2600463

Fossil resource scarcity USD2013 3,09E+03 1,10E+03 1990,5259 64,4062537

Water consumption, Human health DALY 6,39E-01 6,39E-01 0,00023429 0,03663673

Water consumption, Terrestrial ecosystem species.yr 3,89E-03 3,89E-03 1,4247E-06 0,03663673

Water consumption, Aquatic ecosystems species.yr 1,74E-07 1,74E-07 6,3743E-11 0,03663673

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Table 14: Characterization by assembly groups without shipping container.

Impact category Unit Heating Perch food Equipment Electricity Total Heating (%) Perch food (%) Equipment(%) Electricity(%)

Global warming, Human health DALY 8,92E-02 3,34E-02 1,26E-01 9,11E-03 2,58E-01 34,54148962 12,95785383 48,97133232 3,529324233

Global warming, Terrestrial ecosystems species.yr 2,69E-04 1,01E-04 3,81E-04 2,75E-05 7,79E-04 34,5368121 12,95911843 48,97611417 3,527955301

Global warming, Freshwater ecosystems species.yr 7,35E-09 2,76E-09 1,04E-08 7,51E-10 2,13E-08 34,54327284 12,95757919 48,97029496 3,528852999

Stratospheric ozone depletion DALY 5,14E-05 1,28E-05 4,85E-05 2,64E-05 1,39E-04 36,93681756 9,225084282 34,86401706 18,97408109

Ionizing radiation DALY 1,83E-06 2,29E-04 8,66E-04 1,65E-06 1,10E-03 0,166867642 20,85767903 78,82491524 0,150538087

Ozone formation, Human health DALY 7,26E-05 5,46E-05 2,06E-04 8,70E-05 4,21E-04 17,27184729 12,97982511 49,05379931 20,69452829

Fine particulate matter formation DALY 5,90E-03 2,41E-02 9,12E-02 3,54E-03 1,25E-01 4,725375999 19,34073783 73,09348515 2,840401021

Ozone formation, Terrestrial ecosystems species.yr 1,03E-05 7,81E-06 2,95E-05 1,25E-05 6,01E-05 17,19976474 12,9856652 49,07587336 20,7386967

Terrestrial acidification species.yr 1,05E-05 1,45E-05 5,48E-05 1,23E-05 9,21E-05 11,44898934 15,74152494 59,49102254 13,31846318

Freshwater eutrophication species.yr 4,39E-06 3,46E-07 1,31E-06 4,30E-08 6,09E-06 72,12085458 5,685679579 21,48731732 0,70614853

Terrestrial ecotoxicity species.yr 6,94E-07 6,82E-07 2,58E-06 2,79E-08 3,98E-06 17,42562835 17,13128622 64,74383106 0,699254366

Freshwater ecotoxicity species.yr 3,26E-05 9,75E-08 3,68E-07 1,93E-08 3,31E-05 98,53293865 0,294745013 1,113913379 0,058402962

Marine ecotoxicity species.yr 6,43E-06 2,36E-08 8,91E-08 4,06E-09 6,55E-06 98,2169131 0,360122404 1,360990474 0,061974021

Human carcinogenic toxicity DALY 1,84E-02 1,06E-03 3,99E-03 1,42E-05 2,34E-02 78,38740582 4,509444695 17,04244253 0,060706958

Human non-carcinogenic toxicity DALY 1,88E-01 1,58E-03 5,97E-03 3,16E-04 1,96E-01 95,98807249 0,805758195 3,045146493 0,161022818

Land use species.yr 2,53E-06 1,08E-05 4,08E-05 2,92E-05 8,34E-05 3,029947113 12,95839139 48,97308565 35,03857585

Mineral resource scarcity USD2013 1,07E+01 9,71E+00 3,67E+01 3,01E-01 5,74E+01 18,62954049 16,91601831 63,92970766 0,52473353

Fossil resource scarcity USD2013 1,02E+03 1,53E+03 5,80E+03 1,11E+03 9,47E+03 10,78720006 16,20758423 61,25257728 11,75263842

Water consumption, Human health DALY 6,39E-01 6,38E-01 2,41E+00 2,05E-03 3,69E+00 17,30849393 17,29070838 65,34520168 0,055596012

Water consumption, Terrestrial ecosystem species.yr 3,89E-03 3,88E-03 1,47E-02 1,25E-05 2,25E-02 17,30849417 17,29070887 65,34520094 0,055596014

Water consumption, Aquatic ecosystems species.yr 1,74E-07 1,74E-07 6,56E-07 5,59E-10 1,00E-06 17,30849439 17,29070826 65,34520133 0,055596013

Table 15: Damage assessment by assembly group without shipping container.

Damages Unit Heating Perch food Equipment Electricity Total Heating Perch food Equipment Electricity

Human health DALY 9,41E-01 6,99E-01 2,64E+00 1,52E-02 4,30E+00 21,89812173 16,26820421 61,48102379 0,352650272

Ecosystems species.yr 4,22E-03 4,02E-03 1,52E-02 9,40E-05 2,35E-02 17,95610953 17,08318861 64,5609735 0,399728364

Resources USD2013 1,03E+03 1,54E+03 5,84E+03 1,11E+03 9,53E+03 10,83447922 16,21185517 61,26871691 11,68494869