Shine Medleg 18 JAN

7/25/2019 Shine Medleg 18 JAN

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THE FULL 9 STEPPED CYCLE OF PROTON CONDUCTANCE INSIDE HUMAN BODY PROPOSED

BY M.AMBAGA, INCLUDING THE DONATORS + MEMBRANE-REDOX POTENTIALS THREE-

STATE LINE SYSTEM + O2 + DP + PI + H++ NH + MEMB. SPACE = (ATP + HEAT ENERGY +

H2O + NH + MATRIX + CO2! REACTION MEDIUM

U"##$%##' 2)*

).

I$&'&/$.

We at first proposed about that it is existed the full 9 stepped cycle of proton conductance inside

human body, which started from release of proton and electron from food (first stage) ended by

final accumulation of free protons in the form of HbH (O2-Hb) inside of erythrocytes, allowing to

promotion of uptae oxygen by human body and remo!e of carbon dioxide from human body (end 9

stage)" Hydrogen ion, strictly, the nucleus of a hydrogen atom separated from its accompanying

electron" #he hydrogen nucleus is made up of a particle carrying a unit positi!e electric charge, called

a proton" #he isolated hydrogen ion, represented by the symbol H$, is therefore customarily used to

represent a proton"

T01 "" 9 3&1441 5"1 4'&$ $$1 /$3/1 06#$ %5 4'431 %5 M.A6%#7#,

/$"/$7 &01 D$#&'3 + 616%'#$1-'18 4&1$&/#"3 &0'11-3&1 "/$1 353&16 + O2 + DP + P/ + H+

+ $H + 616%. 34#1 = (ATP + 01#& 1$1'75 + H2O + $H + 6#&'/8 + CO2! '1#&/$ 61/6.

%" &elease of proton,electron from food

(carbohydrate,aminoacids, fatty acids)

2" #ransfer of proton,electron to 'H as

hydrogen atom

*" #ransfer of proton,electron to +o as

hydrogen atom

-"#ransfer of elecctronto cytochrom . withoutaccompanying proton

/"#ranslocation of

proton tointermembrane space

of mitochondriawithout accompanying

electron

0" .reation of proton

gradient in the

intermembrane space of

mitochondria and following

transfer of proton to matrix

through #1 synthase

" 3ormation of metabolic

water in the

mitochondrian matrix by

oxidation of proton by

molecular oxygens i"e, by

protonation of molecular

oxygen by matrix proton

4" iffusion of metabolic

water through plasms

membrane of red blood

cells with participation of

a5uaporin protein channels

9" 3ormation of free protons from

metabolic water again by reaction as H2.O*6H$H.O* (H2.O* formed from metabolic

water), proton combine with hemoglobin

(generation of HbH) which promotes the

release of oxygen from hemoglobin, oxygen

diffusion to cells, proton released from

hemoglobin promotes uptae of oxygen by

hemoglobin" .arbonic anhydrase cataly7esthe formation of .O2 from H2.O* and .O2diffuse out in the al!eoli"


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T01 "" 9 3&1441 5"1 4'&$ $$1 /$3/1 06#$ %5 4'431 %5 M.A6%#7# "

/$"1 30 1"" :$$ 61%"/ 4#&0#53 #3 7"5"53/3, 41$&31 40340#&1 4#&0#5, ;'1%3

5"1, %1& 8/#&/$ #&&5 #/3, #6/$ #/ 8/#&/$.

#he formation of free protons from metabolic water again by reaction as H 2.O*6H$H.O*

(H2.O*formed from metabolic water), proton combine with hemoglobin (generation of HbH) which

promotes the release of oxygen from hemoglobin, oxygen diffusion to cells, proton released from

hemoglobin promotes uptae of oxygen by hemoglobin, the formation of .O2 from H2.O* underaction of carbonic anhydrase, diffusion of .O2in the al!eoli in the end 9 stage e!entually lead to the

release of proton and electron from food substrates as carbohydrate, amino acids, fatty acids at first

stage" 8n such way end 9 stage of the full 9 stepped cycle of proton conductance inside human body

is connected with first % stage"

#his is reason why we use thermin as full closed 9 stepped cycle of proton conductance

inside human body, started from food substrates as carbohydrate, amino acids, fatty acids and

ended by oxygen and carbon dioxide i"e" a set of e!ents or actions in the full closed 9 stepped cycle

of proton conductance inside human body happened again and again in the same order, repeating

series of e!ents"

T01 1


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" #he full 9 stepped cycle of proton conductance inside human body proposed by "mbaga and all

regulations, which functioned with participation of .O2, H2O and H2.O*, H.O*"

/" #he full 9 stepped cycle of proton conductance inside human body proposed by "mbaga and all

biosynthesis process, which functioned with participation of '1H"

0" #he full 9 stepped cycle of proton conductance inside human body proposed by "mbaga and

brain function (eton body associated metabolism, action potential)"

" #he full 9 stepped cycle of proton conductance inside human body proposed by "mbaga and

heart function (action potential, myocardial contraction)"


gastric function (free proton dependent formation of H.=)"


renal function (urine H>'a exchange)"

%?" #he full 9 stepped cycle of proton conductance inside human body proposed by "mbaga and

li!er function (ornithine cycle, '1H dependent biotransformation, '1H dependent

biosynthesis)"

%%" #he full 9 stepped cycle of proton conductance inside human body proposed by "mbaga and

li!er function (ornithine cycle, '1H dependent biotransformation, '1H dependentbiosynthesis)"

%2" #he full 9 stepped cycle of proton conductance inside human body proposed by "mbaga and

lung function (oxygen>carbon dioxide exchange mechanism)"

H " 6#:1 &01 /$"1$1 # %#3/ 616%1'3 &01 "" 9 3&1441 5"1 4'&$ $$1

/$3/1 06#$ %5 4'431 %5 M.A6%#7# & '1#&/$ /$&1$3/&5 &0/3 5"1.

). >#$&/&5 05'71$ #&6 (4'&$, 1"1&'$ &71&01' 18/3&1 /$ &01 $#&' /$ &01 /'3&

3G &0/3 5"1 " 6#:1 &01 '16#':#%"1 /$"1$1 & '1#&/$ /$&1$3/&5, %1#31 6'1

05'71$ #&63, 6'1 4'&$ 7'#/1$&3, 6'1 ATP /$ 3/8&0 3G 5"1,6'1 '11 4'&$ /$3/1

1'5&0'5&1 616%'#$1 3''$/$73 (4#"6/&/ #/CH?(CH2)@COOH, 7"31 CH)2O)"

2. >#$&/&5 '11 4'&$3 /$3/1 1'5&0'5&1 616%'#$1 3''$/$73 #& 9 3G

5"1 " 6#:1 &01 '16#':#%"1 /$"1$1 & /3/$ 8571$ & )@ &'/""/$ 1""3 /.1. 6'1 '11

4'&$3 /$3/1 1'5&0'5&1 616%'#$1 3''$/$73 6'1 8571$ 1"/


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. T01 4'1, 6'1 '11 C>, 6'1 8//1

5&0'61 C, 6'1 8571$ 1#" & 6'1 /$&1$3/&5 4'&$ $$1, 6'1 '11 HADH,

"133 8//1 C>, 6'1 '11 C>, "133 8//1 5&0'61 C, "133 8571$ 1#" & "133

/$&1$3/&5 4'&$ $$1, "133 '11 HADH, "133 8//1 C>, "133 '11 C>, "133

8//1 5&0'61 C 1#" & "133 71$1'#&/$ 4'&$ 7'#/1$&3 #& -3G &0/3 5"1.

T01 "" 9 3&1441 5"1 4'&$ $$1 /$3/1 06#$ %5 4'431 %5 M.A6%#7# #$

'"$7, 6:0'/3, %#7#$ 1 T'#/&/$#" 61//$1.

8ncrease of intensity of process at last 9 step of this cycle in the form of rise of uptaeoxygen by human body are accompanied with rise of intensity of release of proton and electron

from food at first % step of this cycle, this process in #raditional medicine coded by fire element and

abstract mhris thermin"

ll these processes are connected with pre!ailence of fluid alpha state with high oxidation

potentials in the membrane-redox potentials three-state line system of :onators $ membrane-

redox potentials three-state line system $ O2 $ ;1 $ 1i $ H$ $ nH $ memb" space 6 (#1 $ heat

energy) $ H2O $ nH $ matrix $ .O2< reaction medium of human body"

ecrease of intensity of process at last 9 step of this cycle in the form of decline of uptae

oxygen by human body are accompanied with decline of intensity of release of proton and electron

from food first % step of this cycle, this process in #raditional medicine coded by water, earth

element and abstract badgan thermin"

ll these processes are connected with pre!ailence of solid betta state with high reducti!e

potentials in the membrane-redox potentials three-state line system of :onators $ membrane-

redox potentials three-state line system $ O2 $ ;1 $ 1i $ H$ $ nH $ memb" space 6 (#1 $ heat

energy) $ H2O $ nH $ matrix $ .O2< reaction medium of human body"

ecrease of uptae oxygen by human body at last 9 step of this cycle and decline of 5uantity

of food at first % step of this cycle are accompanied with decline of intensity of formation of proton

gradient with following decrease of #1 formation and heat energy generation at sixth step of this

cycle, this process in #raditional medicine coded by rlung element and abstract rlung thermin"

T01 "" 9 3&1441 5"1 4'&$ $$1 /$3/1 06#$ %5 4'431 %5

M.A6%#7#, /$"/$7 &01 D$#&'3 + 616%'#$1-'18 4&1$&/#"3 &0'11-3&1 "/$1 353&16 + O 2 +

DP + P/ + H++ $H + 616%. 34#1 = (ATP + 01#& 1$1'75 + H2O + $H + 6#&'/8 + CO2! '1#&/$

61/6.

ll these processes are connected with pre!ailence of gamma state with low oxidation and

reducti!e potentials in the membrane-redox potentials three-state line system of :onators $

membrane-redox potentials three-state line system $ O2 $ ;1 $ 1i $ H$$ nH $ memb" space 6 (#1

$ heat energy) $ H2O $ nH $ matrix $ .O2< reaction medium of human body"

%" 1rotonation capacity of erythrocyte represent the capacity of this cell to form Hb-H with

participation of free protons, generated by reaction as H2.O*6H$H.O*(@eeley natomy, 1hysiology,


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p"4/%, fig"2*"%9) allowing to release of oxygen from Hb-O2, released in such way oxygen diffused to

all cells"

2" 1rotonation capacity of erythrocyte also represent the capacity of this cell to form H2.O*

by reaction as H2.O*6H$H.O* (@eeley natomy, 1hysiology, p"4/%, fig"2*"%9) with participation of

free protons, allowing to form .O2(H2.O*6H2O$.O2) released in such way carbon dioxide exhalated

from body"

*" 1roton and electron releasing capacity of serum represent the capacity of serum en7ymes,

which appeared as uptae of proton and electron from food at first stage, existed in the full 9

stepped cycle of proton conductance inside human body"

" #he 5uantity of protoni7ed compund represent the 5uantity le!el of energetic substrates

accumulated inside human body ready to gi!e proton and electron to the full 9 stepped cycle of

proton conductance inside human body i"e" high le!el, medium optimal le!el, low le!el (no enough

to ensure organism need)"

/" 8n case of our in!estigation H.O* and Hb-O2 of erythrocyte was substitued by nitroblue

tetra7olim and diphenyl picril hydra7il (11H)"

T01 "/1 0/3&'5 4'133 $


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#he membrane-based mechanism for maing #1 consists of two lined stages, both are

carried out by protein complexes in the membrane"

SG )"

Flectrons deri!ed from oxidation of food molecules or from other sources are transferred along a

electron carriers called an electron-transport chain - embedded in the membrane" #hese electron

transfers release energy that is used to pump protons (H$) deri!ed from foods"

SG 2.

1rotons (H$) flows bac down its electrochemical gradient through protein complex called #1

synthase, which catalyses the energy re5uiring synthesis #1 from 1 and inorganic phosphate"

#his ubi5utious en7yme ser!es the role of turbine, permitting the proton gradient to dri!e the

production of #1"

.hemiosmotic coupling (osmotic from the Dree - to push) first e!ol!ed in bacteria"

erobic eucaryotic cells appear to ha!e adopted the bacterial chemiosmotic mechanisms intact, first

by engulfing aerobic bacteria to form mitochondria and somewhat later - in the lineages leading to

algae and plants by engulfing cyanabacteria to form chloroplasts"

Cy examining the lifestyles of !ariety of single celled organisms - including those that resemble our

early ancestors we can began to see the role that chemiosmotic coupling has played in the rise of

complex eucaryotes and in the de!elopment of all life on Farth"

=ife on earth has e!ol!ed o!er billion years" With the e!olution of the membrane-based process of

photosynthesis more than * billion years ago, organism were no longer dependent on performed

organic chemicals"

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Shine Medleg 18 JAN