Cryo CellCryo Cell

SETTING UP OF IUI LABSETTING UP OF IUI LAB

Dr. Suryakant HayatnagarkarDr. Suryakant Hayatnagarkar

Cryo Cell India Pvt. Ltd.Cryo Cell India Pvt. Ltd.

Cryo CellCryo Cell

Rationale for doing I.U.IRationale for doing I.U.I

Optimization of gamete availability at the Optimization of gamete availability at the site of fertilizationsite of fertilization

Reduction in Distance traveled by SpermReduction in Distance traveled by Sperm

Tackling of occult Seminal problemsTackling of occult Seminal problems

Optimization of ovulation & EndometriumOptimization of ovulation & Endometrium

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Ideal Goals of Sperm Ideal Goals of Sperm ProcessingProcessing

Select highest number of motile spermsSelect highest number of motile sperms

Remove Seminal Plasma & ProstaglandinsRemove Seminal Plasma & Prostaglandins

Remove dead , damaged & abnormal spermsRemove dead , damaged & abnormal sperms

Remove white cells & round cellsRemove white cells & round cells

Induce Capacitation & HyperactivationInduce Capacitation & Hyperactivation

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IUI is an effective,non invasive, IUI is an effective,non invasive,

relatively simple & inexpensive relatively simple & inexpensive

method of treatment. It can be method of treatment. It can be

provided easily in simple setups. provided easily in simple setups.

Careful selection & counseling of Careful selection & counseling of

couples is essential.couples is essential.

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SETTING UP IUI LABSETTING UP IUI LAB

Essential requisites of setting up Essential requisites of setting up ART servicesART services

Good lab designGood lab designGood service deliveryGood service delivery

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SETTING UP IUI LABSETTING UP IUI LAB

PlacePlaceEquipmentEquipmentPersonnelPersonnelFacilitiesFacilities

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PLACEPLACEOne room One room

preferably 100 preferably 100 sq feetsq feet

Air conditioned Air conditioned Away from Away from

busy areas, dustbusy areas, dustPreferably in Preferably in

basementbasementNear OT or Near OT or

insemination insemination roomroom

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SECTIONS IN IUI LABORATORYSECTIONS IN IUI LABORATORYSemen analysis and processing Semen analysis and processing

laboratorylaboratorySemen sample collection cabin Semen sample collection cabin

(Masturbatory)(Masturbatory)IUI procedures roomIUI procedures room

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DESIGNING OF LABDESIGNING OF LAB

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OTHER ANCILLARY AREASOTHER ANCILLARY AREAS

Reception and waiting areaReception and waiting areaSubfertility clinicSubfertility clinicMale clinic/ Andrology clinicMale clinic/ Andrology clinicDoctor’s cabinDoctor’s cabinTransvaginal Ultrasonography roomTransvaginal Ultrasonography room

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TESTING EQUIPMENTTESTING EQUIPMENTBinocular Microscope Binocular Microscope

OR OR

Trinocular microscopeTrinocular microscopeMicroscope should have camera Microscope should have camera

port and 20x objectiveport and 20x objective

Phase contrast accessory would Phase contrast accessory would be ideal for semen analysis be ideal for semen analysis laboratorylaboratory

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TESTING EQUIPMENTTESTING EQUIPMENT SPERM COUNTING CHAMBERSPERM COUNTING CHAMBER

Neubaur’s ChamberNeubaur’s Chamber

Cryocell sperm counting chamberCryocell sperm counting chamber

Makler chamberMakler chamber

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PROCESSING EQUIPMENTPROCESSING EQUIPMENTCENTRIFUGECENTRIFUGE

Swing out rotorSwing out rotorRPM meterRPM meterTimerTimerCooling/ auto breaks not Cooling/ auto breaks not

essentialessential

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PROCESSING EQUIPMENTPROCESSING EQUIPMENT INCUBATORINCUBATOR

TEST TUBE WARMERTEST TUBE WARMERDigital indicator and PLC Digital indicator and PLC

controller desirablecontroller desirableCOCO22 incubation not essential incubation not essential

for IUI work.for IUI work.

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ANCILLARY EQUIPMENTANCILLARY EQUIPMENT

Laminar Laminar Flow Flow BenchBench

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ANCILLARY EQUIPMENTANCILLARY EQUIPMENT

RefrigeratorRefrigeratorFor storage of For storage of media and kitsmedia and kits

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ACCESSORIES FOR ACCESSORIES FOR PROCESSINGPROCESSING

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Media Used In Sperm Media Used In Sperm ProcessingProcessing

Use media manufactured by reputed manufacturer, do Use media manufactured by reputed manufacturer, do not use from cottage manufacturer or fly-by-night not use from cottage manufacturer or fly-by-night operatorsoperators

Do not use media in open package, or bulk pckings as Do not use media in open package, or bulk pckings as it is likely to be contaminated before finishing.it is likely to be contaminated before finishing.

Insist on quality certification / check for manufacturers Insist on quality certification / check for manufacturers instructions before using.instructions before using.

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Media Used In Sperm Media Used In Sperm ProcessingProcessing

HAM F-10HAM F-10

EBSSEBSS

HUMAN TUBAL FLUID MEDIUMHUMAN TUBAL FLUID MEDIUMAdditivesAdditives

PentoxyphylinePentoxyphylineAlbuminAlbumin

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Advantages of Density Gradient Advantages of Density Gradient CentrifugationCentrifugation

1. Can be used for both normal and oligospermic samples1. Can be used for both normal and oligospermic samples

2. Concentrates highly motile sperm in the pellet.2. Concentrates highly motile sperm in the pellet.

3. Effectively removes seminal plasma, round cells and3. Effectively removes seminal plasma, round cells anddead spermdead sperm

4. Reduces oxidative damage to sperm.4. Reduces oxidative damage to sperm.

5. Viscous semen samples can be processed.5. Viscous semen samples can be processed.

6. Enhances percentage of spermatozoa with normal 6. Enhances percentage of spermatozoa with normal morphology.morphology.

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PERSONNELPERSONNEL

Trained GynecologistTrained GynecologistTrained TechnicianTrained TechnicianOther support servicesOther support services

– AssistantsAssistants– SonologistSonologist

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WHAT CRYOCELL CAN DO FOR WHAT CRYOCELL CAN DO FOR YOUYOU

Supply Quality reagents and KitsSupply Quality reagents and KitsHelp in designing IUI lab and Help in designing IUI lab and

selecting equipments for the labselecting equipments for the labHelp in procuring the equipmentHelp in procuring the equipment

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WHAT CRYOCELL CAN DO FOR WHAT CRYOCELL CAN DO FOR YOUYOU

Train Gynecologists in proceduresTrain Gynecologists in proceduresTrain Technicians in processing Train Technicians in processing

techniquestechniquesSupply tested Quarantined Frozen Supply tested Quarantined Frozen

Donor Semen samplesDonor Semen samplesTroubleshootingTroubleshooting