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BIOCHEMIEB.V.SETIS™ product of Vervit, Belgium
SETIS™ BioreactorTemporary immersion systems
in plant micropropagation
Plant tissue culture methods have been used for more than 30 years to propagate a large variety of plants. The conventional method, using semisolid (agar) medium, has been widely used because of its simplicity and low costs. However, the huge amount of labor required is a major disadvantage. New technologies have been developed to reduce the handling time, while at the same time increasing the multiplication rates and plant quality. As a result, the Temporary Immersion System (TIS), was created in the 80s.
The principle of TIS technology is simply immersing the plant material into liquid growth media for short periods of time. These immersions are sufficient for the plant’s nutrients uptake. TIS technology profits from the advantages of liquid cultures, while growingtheplantsunderhighgas-exchangeenvironments.
Advantages of TIS technology- Production of more plants per square meter - Higher multiplication rates - Use of liquid medium reduces agar costs - Higher nutrient uptake - Reducedmanipulationsandlabor- Forced aeration increases growth and biomass production - Improved plant quality- Automatization
There are various TIS bioreactor conformations, which have been described by Etienne & Berthouly (2002). From those, the twin-bottles principle has been the most used in commercial micropropagation.
The SETIS™ bioreactor is based on this principle, using separatedvessels for plant material and growth media, but with an optimized design and technology. SETIS™ is a standard and ready-to-use system for large-scale plant micropropagation.
The SETIS™ bioreactor and parts Composedoftwovessels:- Culturevessel(5.6liters)withø80mmneckopening.- Mediavessel(4liters)withø50mmneckopening.
The culture vessel is positioned on top of the media vessel, making the system very compact and easier to use.
Thevesselscontaintwoexternalhose-barbconnectorsaspartof the vessel body. There are no internal components in the vessels.
The vessels are closed via screw caps (80 and 50 mm diameter), using a silicone gasket in between to assure perfect sealing.
The culture vessel has a flat bottom area of 432 cm2, allowing a high capacity and optimal plant material distribution.
Externalmeasurementsassembled:308x180x250mmBasemeasurement:240x180mm
The bioreactor requires horizontal shelving for optimal drainage. The inclination of the culture vessel allows self-drainage using gravity.Amaximumof3litersofgrowthmediumiscompletelydrainedinapproximately5min.
Working air pressure: 0.1-0.3 bar
The capacity of the bioreactor depends on the plant species, ranging from 300 to 1200 plants per bioreactor.
All components (vessel opening, connectors and air filters), are located on the front side of the bioreactor, simplifying access once placed on the shelf.
Stationary phase
NAGELENAGELE
Compressed air
Flow direction of growth media or air
Immersion phase
Compressed air is suppliedinto the media vessel in orderto transfer growth mediato the upper culture vessel.
End of immersion
Growth media is returnedto the media vessel bygravity force.
Ventilation phase
Compressed air issupplied into the culturevessel to renew its aircomposition.
The bioreactor includes stackable elements between both vessels to assure a perfect positioning of the culture vessel on top of the media vessel, and to avoid its diaplacement during operation. Two handles are positioned at both sides of the media vessel to easily carry the assembled bioreactor.
The bioreactor is composed of only three materials, polycarbonate (vessels), polypropylene (screw caps and air filters) and silicone (gaskets and tubing). All of these materials withstand autoclave sterilization and gamma irradiation.
The design of the bioreactor, with its free and curved top, and thehightransparencyofthevessels,allowsamaximumlightincidence into the plants. Note: Follow the instructions described in “User Manual” (www.setis-systems.be), for proper bioreactor utilization.
Rack setup
CustomizedracksforSETIS™bioreactorsareavailablewithdimensionsof1,5x0,7m.Eachrackhasfourshelveswithatotal of 64 SETIS™ bioreactors (8 rows of 8 bioreactors), with the possibility to regulate the height of each shelf.
Bioreactors are placed side-by-side and back-to-back, guarantee-ingamaximumuseoftheshelfarea.Allconnectorsarelocatedat the rack periphery, granting easy access by the operator, and without interfering with the lighting.
The racks include the complete pneumatic tubing. In this case, one line connected to the media vessel to allow immersion and a second line connected to the culture vessel to allow active ventilation.
Bioreactors can be separately controlled via the software in sets of 1, 2, and 4 rows of 8 bioreactors each.
Lighting is installed optionally according to customer requirements.
Software and controls
This is an important component of any advanced TIS installation. The link between pneumatic elements and computer intelligence has to be perfect in order to precisely control the bioreactor’s operation. This way, the operator enjoys the advantages of an automated installation.
SETIS™ has built its own software/controls, based on state-of-the-artPLCprogrammingandhighqualitypneumaticcomponents.
Some of the software features:• Controlofimmersionandventilationphases• Integratedlightingcontrol• Unlimitednumberofcontrolpoints• Automatictimefrequenciescalculation• Optimizationandreductionofairconsumption• Createandsaveyourownprograms• Flexibleanddynamicinstallation• Backups• Controlpointsandalarms• Web-basedaccesstocontrolthelabfromanylocation
worldwide• Easyanduser-friendlyinterface• Andmanymore…
Cultivating different plant species
The SETIS™ bioreactor has been used to successfully cultivate many different plant species, such as sugarcane, banana, potato, eucalyptus, paulownia and orchids.
With its adequate culture volume, optimal light entry, complete drainage and improved ventilation, the SETIS™ bioreactor is an ideal system to avoid vitrification problems and increase plant quality.
SETIS™ is more than a bioreactor
The concept of providing an optimized bioreactor to the plant micropropagation industry is not everything.
Additionally, there is a range of products and services offered un-der the SETIS™ brand name, such as bioreactor accessories, side equipments and technology transfer.
SETIS™ comprises all necessary elements for designing and initiating a high-tech Temporary Immersion System laboratory. Advantages of the SETIS™ bioreactor
• Simpleandminimalist• Compact• Secure• Maximumsurfaceutilization• Easyassemblyandhandling• Optimallabsetup• Standard• Ready-to-use
SETIS™ bioreactorSE-S01, B6000
1- 10 Units e 93,5011- 300 Units e 85,00
Each bioreactor is supplied with a culture vessel and a medium vessel including their intergrated connectors, both caps, two sealing gaskets for the caps, two air filters ( Ø 50 mm) and 40 cm silicone tube (6/9 mm).
All components can be purchased separately.
Transport costsThese goods are shipped Ex Works (EXW) to all destinations.Transportation charges will vary with the destination, weight, and content of each shipment and will be subcharged accordingly on the corresponding invoice.
Packaging detailsSingleunitpackage32x28x19cm(1.8kg.)Fiveunitpackage98x33x29cm(9.0kg.)
HS tariff code 39231000
DUCHEFA BIOCHEMIE B.V.A. Hofmanweg 712031 BH HaarlemThe NetherlandsTelephone: +31 - (0)23 - 531 90 93Telefax: +31-(0)23-5318027Mobile: +31-(0)634782972E-mail: [email protected]
BIOCHEMIEB.V.
Culturevessel
Mediumvessel
SETIS™ product of Vervit, Belgium
