Serial DilutionsSerial DilutionsExample!Example!

0.2 g/mL NaCl0.2 g/mL NaCl

Stock SolutionStock Solution

Make a 0.2-g/mL NaClNaCl STOCKSTOCK solutionsolution

- Add 2 g of sodium chloride to beaker and add distilled water to 10 mL

Get as many test tubes (minus two) as you have Petri dishes for

your experiment

Add 5 mL of distilled water to each of you test

tubes

Add 5 mL of Stock Solution to

first test tube.

Mix the first Tube

0.1 g/mL 0.1 g/mL NaClNaCl

Take 5 mL of first tube and add to second

tube

Mix

0.05 0.05 g/mL g/mL NaClNaCl

0.1 0.1 g/mL g/mL NaClNaCl

Repeat and remove 5 ml

from last tube and discard0.025 0.025

g/mL g/mL NaClNaCl

0.05 0.05 g/mL g/mL NaClNaCl

0.0125 0.0125 g/mL g/mL NaClNaCl

0.1 0.1 g/mL g/mL NaClNaCl

0.00625 0.00625 g/mL NaClg/mL NaCl

0.00313 0.00313 g/mL NaClg/mL NaCl

0.0156 0.0156 g/mL NaClg/mL NaCl