Sequencing-ready libraries. Simply delivered.NeoPrep™ Library Prep System. Library prep has never been this easy.

Next-gen sequencing deserves next-gen library prep.Sequencing-ready libraries with unrivaled simplicity.Finally, what your lab needs and what you deserve—a radically simple solution that performs the

complete library prep process for you, delivering sequencing-ready libraries, including quantification

and normalization.

Featuring the precision of digital microfluidics and unparalleled ease of use, the entirely

self-contained NeoPrep Library Prep System reduces your hands-on time and achieves

reproducible, high-quality libraries, even with limited starting material.

With an expanding portfolio of assays and a single, streamlined workflow that reduces the need for

ancillary equipment, the versatile NeoPrep System makes next-generation library prep accessible

to all labs and users.

Designed for use with all Illumina sequencing systems and informatics tools, the NeoPrep System

is an integral part of our seamless next-generation sequencing (NGS) workflow.

Transformative technology: the NeoPrep System library cardDigital microfluidics, an innovative fluid-handling technology, precisely manipulates

droplets to perform all library prep processes within the tightly controlled environment

of the NeoPrep System library card.

Radical simplicity.Far fewer steps. Far less hands on.With just three simple steps in the workflow, the NeoPrep System frees you

from the burden of preparing, quantifying, and normalizing libraries. Convenient

overnight run capabilities let you walk away from library prep and return to

sequencing-ready libraries in the morning.

From system design to installation and intuitive operation, every aspect of the

NeoPrep System is optimized for efficiency and ease of use.

Free your time and resources

• Decreases pipetting by ~85%*

• Reduces hands-on time per run to just 30 minutes

• Saves ~11 weeks per year of manual work†

* Calculations based on a similar assay using manual library preparation methods.† Calculations based on 2000 samples per year using a similar assay.

Excellent reproducibility. High-quality libraries, even with limited starting material.The NeoPrep System replaces almost every manual step with the precisely controlled liquid

handling of digital microfluidics, significantly reducing the potential for human error. Low input

requirements, uncompromised quality, and proven reproducibility define the new gold standard

in library preparation.

Low input. High performance.

• Accepts 4× to 10× lower input amounts vs manual library preparation methods (assay dependent)

• Attains equivalent performance specifications to manual library preparation kits

• Prepares 16 libraries per run

• Processes up to 7000 libraries per year*

* Calculations based on 2 NeoPrep System runs per day at 45 work weeks per year.

Refer to www.illumina.com/neoprepsystem for a list of currently available assays

Remarkable versatility. Broad range of assays. Accessible to all labs. The NeoPrep System can perform most library preparation assays. Because workflows are

virtually the same from assay to assay, you can adopt new assays quickly. NeoPrep Library

Prep Kits are optimized to run out of the box, and they also provide the flexibility to modify

run parameters such as insert sizes, PCR cycles, and fragmentation times.

Streamline operations with the NeoPrep System

• Minimizes the need for ancillary equipment and user-supplied consumables

• Reduces the experience and skills required for library preparation

• Includes DNA and RNA assays from our industry-leading library prep portfolio

Transcriptome Targeted resequencingWhole genome

Library prep

In addition to manual

options, Illumina offers

the NeoPrep System for

reproducible sequencing-

ready libraries with just

30 minutes of hands-on

time per run.

Sequencing

With power for every scale,

Illumina offers a complete

portfolio of next-generation

sequencing (NGS) solutions

that are accessible for

every study and every lab.

Informatics

Intuitive informatics tools

provide critical insights.

Essential data can be

transferred, stored,

analyzed, and shared

securely in BaseSpace®

Onsite or in the

BaseSpace Cloud.

Optimize with simply smart NGS workflow solutions. One seamless process. One complete resource.From library prep to sequencing and informatics, Illumina offers seamless NGS workflow

solutions that optimize your process from start to finish and accelerate your progress.

As the innovators of next-generation technology, we’re here to deliver the experience

and expertise to help propel your success.

Illumina • 1.800.809.4566 toll-free (US) • +1.858.202.4566 tel • techsupport@illumina.com • www.illumina.com

FOR RESEARCH USE ONLY© 2015 Illumina, Inc. All rights reserved. Illumina, BaseSpace, NeoPrep, the pumpkin orange color, and the streaming bases design are trademarks of Illumina, Inc. and/or its affiliate(s) in the US and/or other countries. All other names, logos, and other trademarks are the property of their respective owners. Pub. No. 970-2014-006 Current as of 12 February 2015

World-class solutions. A community of support.From sample prep, library prep, arrays, and sequencing to informatics,

Illumina next-generation solutions empower researchers and clinical researchers

across the globe to find the answers they seek.

When you join the Illumina community, you become part of a dynamic scientific

movement that includes thousands of researchers and industry thought leaders.

Throughout the year, we host user group meetings, symposia, consortia, online

forums, and other initiatives—all designed to bring the best minds together to

share ideas and advance science.

In addition to on-site training, ongoing support, and phone consults, we offer

webinars and courses at various Illumina locations. We’re here with all the

resources you need to accelerate progress.

A global genomics leader, Illumina delivers complete next-generation sequencing workflow solutions to the

basic and translational research communities. Illumina technology is responsible for generating more than

90% of the world’s sequencing data. Through collaborative innovation, Illumina is fueling groundbreaking

advancements in the fields of oncology, reproductive health, genetic disease, microbiology, agriculture,

and forensic science.

Discover how your lab can benefit from the NeoPrep Library Prep System. www.illumina.com/neoprepsystem.