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Sequence & course material repository
http://gfx.dnalc.org/files/evidence
• Annotation (sequences & evidence)• Manuals (DNA, Subway, Apollo, JalView)• Presentations (.ppt files)• Prospecting (sequences)• Readings (Bioinformatics tools, splicing, etc.)• Worksheets (Word docs, handouts, etc.)• BCR-ABL (temporary; not course-related)
Manifestations of a Code
Genes, genomes, bioinformatics and cyberspace – and the promise they hold
for biology education
Plants are amazing – and so are their genomes
Largest flower (~ 1m) Oldest plant (> 5000 years) Tallest organism (> 100m)
Slide: ASPB, 2009
A GENOME is all of a living thing’s genetic material.
The genetic material is DNA (DeoxyriboNucleic Acid)
DNA, a double helical molecule, is made up of four nucleotide “letters”:
A-- --G
T-- --C
What is a genome?
Slide: JGI, 2009
Just as computer software is rendered in long strings of 0s and 1s, the GENOME or “software” of life is represented by a string of the four nucleotides, A, G, C, and T.
To understand the software of either - a computer or a living organism - we must know the order, or sequence, of these informative bits.
What is sequencing?
Slide: JGI, 2009
Exciting?>mouse_ear_cress_1080 GAAATAATCAATGGAATATGTAGAGGTCTCCTGTACCTTCACAGAGATTCTAGGCTGAGAGCAGTGCATATAGATATCTTTCGTACTCATCTGCTTTTTCTGGTCTCCATCACAAAAGCCAACTAGGTAATCATATCAATCTCTCTTTACCGTTTACTCGACCTTTTCCAATCAGGTGCT TCTGGTGTGTCTACTACTATCAGTTTTAGGTCTTTGTATACCTGATCTTATCTGCTACTG AGGCTTGTAAAAGTGATTAAAACTGTGACATTTACTCTAAGAGAAGTAACCTGTTTGATGCATTTCCCTAATATACCGGTGTGGAAAAGTGTAGGTATCTGTACTCAGCTGAAATGGTGGACGATTTTGAAGAAGATGAACTCTCATTGACTGAAAGCGGGTTGAAGAGTGAAGATGGCGTTATTATCGAGATGAATGTCTCCTGGATGCTTTTATTATCATGTTTGGGAATTTACCAAGGGAGAGGTATCAGAATCTATCTTAGAAGGTTACATTTAGCTCAAGCTTGCATCAACATCTTTACTTAGAGCTCTACGGGTTTTAGTGTGTTTGAAGTTTCTTAACTCCTAGTATAATTAGAATCTTCTGCAGCAGACTTTAGAGTTTTGGGATGTAGAGCTAACCAGAGTCGGTTTGTTTAAACTAGAATCTTTTTATGTAGCAGACTTGTTCAGTACCTGAATACCAGTTTTAAATTACCGTCAGATGTTGATCTTGTTGGTAATAATGGAGAAACGGAAGAATAATTAGACGAAACAAACTCTTTAAGAACGTATCTTTCAGTTTTCCATCACAAATTTTCTTACAAGCTACAAAAATCGAACTATATATAACTGAACCGAATTTAAACCGGAGGGAGGGTTTGACTTTGGTCAATCACATTTCCAATGATACCGTCGTTTGGTTTGGGGAAGCCTCGTCGTACAAATACGACGTCGTTTAAGGAAAGCCCTCCTTAACCCCAGTTATAAGCTCAAAGTTGTACTTGACCTTTTTAAAGAAGCACGAAACGAAAAACCCTAAAATTCCCAAGCAGAGAAAGAGAGACAGAGCAAGTACAGATTTCAACTAGCTCAAGATGATCATCCCTGTTCGTTGCTTTACTTGTGGAAAGGTTGATATTTTCCCCTTCGCTTTGGTCTTATTTAGGGTTTTACTCCGTCTTTATAGGGTTTTAGTTACTCCAAATTTGGCTAAGAAGAGATCTTTACTCTCTGTATTTGACACGAATGTTTTTAATCGGTTGGATACATGTTGGGTCGATTAGAGAAATAAAGTATTGAGCTTTACTAAGCTTTCACCTTGTGATTGGTTTAGGTGATTGGAAACAAATGGGATCAGTATCTTGATCTTCTCCAGCTCGACTACACTGAAGGGTAAGCTTACAATGATTCTCACTTCTTGCTGCTCTAATCATCATACTTTGTGTCAAAAAGAGAGTAATTGCTTTGCGTTTTAGAGAAATTAGCCCAGATTTCGTATTGGGTCTGTGAAGTTTCATATTAGCTAACACACTTCTCTAATTGATAACAGAAGCTATAAAATAGATTTGCTGATGAAGGAGTTAGCTTTTTATAATCTTCTGTGTTTGTGTTTTACTGTCTGTGTCATTGGAAGAGACTATGTCCTGCCTATATAATCTCTATGTGCCTATCTAGATTTTCTATACAATTGATATTTGATAGAAGTAGAAAGTAAGACTTAAGGTCTTTTGATTAGACTTGTGCCCATCTACATGATTCTTATTGGACTAATCATTCTTTGTGTGAAAATAGAATACTTTGTCTGAACATGAGAGAATGGTTCATAATACGTGTGAAGTATGGGATTAGTTCAACAATTTCGCTATTGGAGAAGCAAACCAAGGGTTAATCGTTTATAGGGTTAAGCTAATGCTCTGCTCTTTATATGTTATTGGAACAGACTATTGTTGTGCCTATCTTGTTTAGTTGTAGATTCTATCTCGACTGTTATAAGTATGACTGAAGGCTTGATGACTTATGATTCTCTTTACACCTGTAGAAGGATTTAAGCTTGGTGTCTAGATATTCAATCTGTGTTGGTTTTGTCTTTCTTTTGGCTCTTAGTGTTGTTCAATCTCCTCAATAGGTATGAAGTTACAATATCCTTATTATTTTGCAGGGACGCACTTGATGCACTCCAGCTAGTCAGATACTGCTGCAGGCGTATGCTAATGACCTTGCATCAACATCTTTACTTAGAGCTCTACGGGTTTTAGTGTGT
Much better
FindGene Families
Generate mathematical
evidence
Analyze large data amounts
Browse in context
Build gene models
Gatherbiological evidence
Annotation workflow
Get DNA sequence
Walk or…
…take DNA Subway
Molecular biology and bioinformatics conceptsRepeatMasker• Eukaryotic genomes contain large amounts of repetitive DNA.• Transposons can be located anywhere.• Transposons can mutate like any other DNA sequence.
FGenesH Gene Predictor• Protein-coding information begins with start, is followed by codons, ends with stop.• Codons in mRNA (AUG, UAA,…) have sequence equivalents in DNA (ATG, TAA,…).• Most eukaryotic introns have “canonical splice sites,” GT---AG (mRNA: GU---AG).• Gene prediction programs search for patterns to predict genes and their structure.• Different gene prediction programs may predict different genes and/or structures.
Multiple Gene Predictors• The protein coding sequence of a mRNA is flanked by untranslated regions (UTRs).• UTRs hold information for the half-lives of mRNAs and regulatory purposes.• Gene > mRNA > CDS.
BLAST Searches• Gene or protein homologs share similarities due to common ancestry. • Biological evidence is needed to curate gene models predicted by computers.• mRNA transcripts and protein sequence data provide “hard” evidence for genes.
How do we find genes?
Search for themLook them up
How do I get to this…
From this…
>mouse_ear_cress_1080 GAAATAATCAATGGAATATGTAGAGGTCTCCTGTACCTTCACAGAGATTCTAGGCTGAGAGCAGTGCATATAGATATCTTTCGTACTCATCTGCTTTTTCTGGTCTCCATCACAAAAGCCAACTAGGTAATCATATCAATCTCTCTTTACCGTTTACTCGACCTTTTCCAATCAGGTGCT TCTGGTGTGTCTACTACTATCAGTTTTAGGTCTTTGTATACCTGATCTTATCTGCTACTG AGGCTTGTAAAAGTGATTAAAACTGTGACATTTACTCTAAGAGAAGTAACCTGTTTGATGCATTTCCCTAATATACCGGTGTGGAAAAGTGTAGGTATCTGTACTCAGCTGAAATGGTGGACGATTTTGAAGAAGATGAACTCTCATTGACTGAAAGCGGGTTGAAGAGTGAAGATGGCGTTATTATCGAGATGAATGTCTCCTGGATGCTTTTATTATCATGTTTGGGAATTTACCAAGGGAGAGGTATCAGAATCTATCTTAGAAGGTTACATTTAGCTCAAGCTTGCATCAACATCTTTACTTAGAGCTCTACGGGTTTTAGTGTGTTTGAAGTTTCTTAACTCCTAGTATAATTAGAATCTTCTGCAGCAGACTTTAGAGTTTTGGGATGTAGAGCTAACCAGAGTCGGTTTGTTTAAACTAGAATCTTTTTATGTAGCAGACTTGTTCAGTACCTGAATACCAGTTTTAAATTACCGTCAGATGTTGATCTTGTTGGTAATAATGGAGAAACGGAAGAATAATTAGACGAAACAAACTCTTTAAGAACGTATCTTTCAGTTTTCCATCACAAATTTTCTTACAAGCTACAAAAATCGAACTATATATAACTGAACCGAATTTAAACCGGAGGGAGGGTTTGACTTTGGTCAATCACATTTCCAATGATACCGTCGTTTGGTTTGGGGAAGCCTCGTCGTACAAATACGACGTCGTTTAAGGAAAGCCCTCCTTAACCCCAGTTATAAGCTCAAAGTTGTACTTGACCTTTTTAAAGAAGCACGAAACGAAAAACCCTAAAATTCCCAAGCAGAGAAAGAGAGACAGAGCAAGTACAGATTTCAACTAGCTCAAGATGATCATCCCTGTTCGTTGCTTTACTTGTGGAAAGGTTGATATTTTCCCCTTCGCTTTGGTCTTATTTAGGGTTTTACTCCGTCTTTATAGGGTTTTAGTTACTCCAAATTTGGCTAAGAAGAGATCTTTACTCTCTGTATTTGACACGAATGTTTTTAATCGGTTGGATACATGTTGGGTCGATTAGAGAAATAAAGTATTGAGCTTTACTAAGCTTTCACCTTGTGATTGGTTTAGGTGATTGGAAACAAATGGGATCAGTATCTTGATCTTCTCCAGCTCGACTACACTGAAGGGTAAGCTTACAATGATTCTCACTTCTTGCTGCTCTAATCATCATACTTTGTGTCAAAAAGAGAGTAATTGCTTTGCGTTTTAGAGAAATTAGCCCAGATTTCGTATTGGGTCTGTGAAGTTTCATATTAGCTAACACACTTCTCTAATTGATAACAGAAGCTATAAAATAGATTTGCTGATGAAGGAGTTAGCTTTTTATAATCTTCTGTGTTTGTGTTTTACTGTCTGTGTCATTGGAAGAGACTATGTCCTGCCTATATAATCTCTATGTGCCTATCTAGATTTTCTATACAATTGATATTTGATAGAAGTAGAAAGTAAGACTTAAGGTCTTTTGATTAGACTTGTGCCCATCTACATGATTCTTATTGGACTAATCATTCTTTGTGTGAAAATAGAATACTTTGTCTGAACATGAGAGAATGGTTCATAATACGTGTGAAGTATGGGATTAGTTCAACAATTTCGCTATTGGAGAAGCAAACCAAGGGTTAATCGTTTATAGGGTTAAGCTAATGCTCTGCTCTTTATATGTTATTGGAACAGACTATTGTTGTGCCTATCTTGTTTAGTTGTAGATTCTATCTCGACTGTTATAAGTATGACTGAAGGCTTGATGACTTATGATTCTCTTTACACCTGTAGAAGGATTTAAGCTTGGTGTCTAGATATTCAATCTGTGTTGGTTTTGTCTTTCTTTTGGCTCTTAGTGTTGTTCAATCTCCTCAATAGGTATGAAGTTACAATATCCTTATTATTTTGCAGGGACGCACTTGATGCACTCCAGCTAGTCAGATACTGCTGCAGGCGTATGCTAATGACCTTGCATCAACATCTTTACTTAGAGCTCTACGGGTTTTAGTGTGT
Meaning?
Mathematical Tools (Code; statistics)
Comparative Tools (Database searches)
What do we know about genes?• Expressed (Transcribed)
– Transcriptional start & termination sites (TXSS, TXTS)– Transcription artefacts (cDNA & ESTs)
• Regulated– Promoters (TATAAA)– Transcription Factor Binding Sites– CpG (Cytosin methylation)
• Meaningful (Translated)– 3n basepairs– Codon usage– Translational start & stop/termination codons (TLSS, TLTS)– Translation artefacts (proteins)
• Spliced– Splice sites (GT-AG)
• Derived (Homology: Paralogy/Orthology)– Search for known genes, proteins (BLAST)
How might this knowledge help to find genes?
• Predict genes– Look for potential starts and stops.– Connect them into open reading frames (ORFs).– Filter for “correct’ length & codon usage.
• Search databases– Known genes: UniGene– Known proteins: UniProt
• Use transcript evidence– cDNA– ESTs– proteins
Operating computationally
• Go to beginning of sequence start SCAN• If ATG register putative TLSS; then
– Move in 3-steps & count steps (=COUNTS)– If 3-step = (TAA or TAG or TGA), register putative TLTS– If register evaluate COUNTS (= triplets)
• If COUNTS < minimum discard; then go behind ATG above and start SCAN
• If COUNTS > maximum discard; then go behind ATG above and start SCAN
• If minimum < COUNTS < maximum record as GENE with TLSS, TLTS; then go behind ATG above and start SCAN.
• Arrive at end of sequence stop SCAN
Find gene families
Mathematical evidence
Analyze large data
sets
Browse in ccontext
Construct gene
models
Annotation workflow
Biological evidence
Browse results
Get/Generate sequence
Annotation Cheat Sheet
• Open existing project or generate new (Red square)
• Run RepeatMasker
• Generate evidence (Predictions, BLAST searches)
• Synthesize evidence into gene models (Apollo)
• Browse results locally and in context (Phytozome)
• Conduct functional analysis (link from Browser)
• Prospect for gene family (Yellow Line from Browser)
• Select region that holds biological gene evidence
• Optimize work space and zoom to region (View tab)
• Expand all tiers (Tiers tab)
• Drag evidence item(s) onto workspace (mouse)
• Edit to match biol. evidence (right-click item for tools)
• Record what was done in Annotation Info Editor
• Assess necessity to build alternative model(s)
• Upload model(s) to DNA Subway (File tab)
A. DNA Subway
B. Apollo
Predictors (mathematical evidence)
• Utilize predominantly mathematical methods (statistical).• Search for patterns
– Some score starts, stops, splice sites (GenScan).– Some score nucleotides (Augustus, FGenesH).
• Few incorporate EST data and/or known genes/proteins.• Require optimization for each new species (training).• Accuracy:
– False positives (scoring non-genes as genes):5% - 50%.– False negatives (missed genes): 5%-40%.– Weak or unable in determining first and last exons, and UTRs.
• Specific for gene models (spliced genes, non-spliced genes).• Specialty predictors (tRNA Scan, RepeatMasker).
Search tools (biological evidence)
• Search sequence databases:– Known genes– Known proteins– cDNAs & ESTs
• Utilize alignment methods (BLAST, BLAT).• Reliability:
– Good in determining gene locations and general gene structures.– Weak in exactly determining exon/intron borders.– Unlikely to correctly determine TXSS and TXTS.– Should be used with cDNA/EST from same species.
mRNA Splicing
During RNA processing internal segments are removed from the transcript and the remaining segments spliced together.
Internal RNA segments that are removed are named introns; the spliced segments are defined as exons.
• Causes mRNA to be “missing” segments present in DNA template and primary transcript.
• Most transcripts in eukaryotes spliced.• Erosion: 1-exon genes (no exons without introns).
Exon ExonIntron
Pre-
mR
NA
5’ Splice Site
3’ Splice SiteReddy, S.N. Annu. Rev. Plant Biol. 2007 58:267-94Of 1588 examined predicted splice sites in Arabidopsis
1470 sites (93%) followed the canonical GT…AGconsensus. (Plant (2004) 39, 877–885)
Canonical splice sites
Multiple splice variants = multiple proteins from the same gene
Alternative Splicing
Not a rare event!!!
- Alternative splice sites C’ and D’ lead to different splice variants- JAZ10.3: premature stop codon in D exon, intact JAS domain- JAZ10.4: truncated C exon, protein lacks JAS domain- JAZ 10 encoded by At5G13220
Example: Jasmonate signaling in Arabidopsis
- Plant hormone; affects cell division, growth, reproduction and responses to insects, pathogens, and abiotic stress factors.
- Jasmonate Signaling Repressor Protein JAZ 10 splice variants JAZ 10.1, JAZ 10.3 and JAZ 10.4 differ in susceptibility to degradation.
- Phenotypic consequences include male sterility and altered root growth.
Example: Disease resistance in tobacco
- Nicotiana tabacum resistance gene N involved in resistance to TMV.- Alternative splicing required to achieve resistance.- Alternative transcripts Ns (short) and NL (long).- NS encodes full-length, NL a truncated protein.- Splicevariants produced by alternative splicing confer resistance (D).- Splicevariants produced by cDNAs do not confer resistance (A, B, C).
Molecular biology and bioinformatics conceptsRepeatMasker• Eukaryotic genomes contain large amounts of repetitive DNA.• Transposons can be located anywhere.• Transposons can mutate like any other DNA sequence.
FGenesH Gene Predictor• Protein-coding information begins with start, is followed by codons, ends with stop.• Codons in mRNA (AUG, UAA,…) have sequence equivalents in DNA (ATG, TAA,…).• Most eukaryotic introns have “canonical splice sites,” GT---AG (mRNA: GU---AG).• Gene prediction programs search for patterns to predict genes and their structure.• Different gene prediction programs may predict different genes and/or structures.
Multiple Gene Predictors• The protein coding sequence of a mRNA is flanked by untranslated regions (UTRs).• UTRs hold information for the half-lives of mRNAs and regulatory purposes.• Gene > mRNA > CDS.
BLAST Searches• Gene or protein homologs share similarities due to common ancestry. • Biological evidence is needed to curate gene models predicted by computers.• mRNA transcripts and protein sequence data provide “hard” evidence for genes.
…take DNA Subway