September 18, 2012

Check the clipboard for your new seat

INTEGRATED SCIENCE DAY6

LEARNING TARGETS1. I can evaluate a laboratory procedure and

analyze the data to form a conclusion

AGENDAKick off question: How could you improve your laboratory skills? (do you need to follow directions better, work on being more precise, write a better conclusion, etc.) 1.Conclusion writing revisited2.Daphnia Lab continued3.Daphnia Lab conclusion4.Turn in formal conclusion for heart rate lab5.Turn in daphnia lab6.Learning log: What does alcohol, caffeine and nicotine do to daphnia heartrate?

HOW TO WRITE A CONCLUSION:

You asked a question, you did an experiment, and you did the experiment a second time. You recorded your results. Now it is time to write your conclusion. The conclusion, plain and simple, is the answer to your question. It should be clear, concise and stick to the point. Resist the temptation to jump to conclusions.  If you were to do your experiment again, would you get the same results? Can there be differences? Why? Ask yourself what happened when you tested your hypothesis. What have you learned from this experiment? What does this experiment tell us

Now for homework-- using the answers to the questions on the heart rate lab, write a final report summarizing your question, research methods and conclusion. Work on your conclusion on a separate piece of paper

until lunch. If you complete the conclusion, staple it to your lab report and turn it into the second period box.

REVIEW OF MICROSCOPE Use your assigned microscope and return it

to its proper place as instructed Always start on low power Make sure your lenses are clean We will be using depression lenses, make

sure your Daphnia is in the well and do not overflow.

BIOASSAYS Dose/Response Bioassays are used to:Dose/Response Bioassays are used to:

Estimate toxicity to humans.Estimate toxicity to humans. Estimate maximum concentrations of specific Estimate maximum concentrations of specific

chemicals allowed to discharge into bodies of chemicals allowed to discharge into bodies of water.water.

Investigate hazardous waste sites. Investigate hazardous waste sites.

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BIOASSAY SPECIESBIOASSAY SPECIES

3 organisms are often used3 organisms are often used DaphniaDaphnia DuckweedDuckweed Lettuce SeedsLettuce Seeds

Expose organisms to chemicals and measure Expose organisms to chemicals and measure how they respond.how they respond.

REMEMBER YOU ARE WORKING WITH A LIVING CREATURE http://www.microscopyu.com/moviegallery/po

ndscum/daphnia/

At the end of each experiment return your daphnia to the Rehabilitation and Recovery area on the demonstration desk. Use a new daphnia for each chemical

DAPHNIA

PART 1 Take a look at your daphnia on low power-use

the sketch I put on the overhead to help sketch and label your daphnia

Look at your daphnia on medium power and sketch and label what you see

Do not use high power with daphia or well plates

PART 2

Make sure to get a new daphnia for each chemical you try

You will need a timer to do the heart rate Make sure to record beats/min Return daphnia to the rehab bowl Clean up your lab station and return your

microscope to the cabinet as directed

DATA Make sure to put your averages into the data

table at my desk Make sure to write down the class average Graph your data using a bar chart, showing

your averages and the class averages

CONCLUSION If time permits fill in the conclusion parts of

the lab. Next class we will use this information and some background information about the drugs to build a more complete conclusion

Turn in your lab report whether you complete the report or not.