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Semi – Rigid Isolator Units
Charles River Laboratories
Consulting & Staffing Services
Terminology
Flexible Film SRI HEPA Port Gasket Magnahelic Gauge
Isolator History & Function
Used since 1950’s Flexible Film or Rigid
contruction Biocontainment
Negative Air Pressure Bioexclusion
Positive Air Pressure “Biosecurity” at Charles
River
Features
Typical forward facing port
Gasket will change color when sealed properly
Gasket Clamp
Hinge
Features
Flexible Front
HEPA Filter
Glove Arm
ExternalFilter
Isolator Supplies
Nitrile Gloves
ElectricalTape
O-Rings
Leak Detection
Snoop® Apply liquid to area
suspected of leak and watch for bubbles
Alternative Mild Soap solution
Air flow
NegativePositive
Keeping it Clean
Wescodyne® Clidox
Bleach Solution Windex®/Cinch®
Peracetic Acid Chemical
Incompatibility Check MSDS
Ergonomics
Your back should always be as straight as possible.
Hydraulic tables allow the user to adjust the height!
Filter Change
Filters should be checked daily
Filters need to be changed when the magnahelic gauge increases by 0.05 (i.e. from 1.0 to 1.5)
Filter Pop Quiz
Which filter assembly needs to be replaced?
Testing for deterioration
This rim should appear green even when stretched.
If small holes or cracks are present glove must be replaced.
Punctures from animal bites or instruments also warrant glove replacement.
Glove and flexible surface checks
•Check the magnahelic gauge•Pull the gloves inside out, pulling on the cuff only•Check for holes, punctures, tears, or stretched areas•Then recheck the magnahelic for proper pressure•Wipe down the outside of the flexible front surface
Semi-Rigid Isolator Daily Checks and Inspections
Magnahelic and Ball Valve
Ball Valve
MagnahelicGauge
Fan Motor
Magnehelic gauge check
•Check gauge and record twice daily•Gauge reads what the pressure is inside the isolator (+.10 or -.10)
•Reads in Inches of Water•Not reading proper pressure report immediately •DO NOT adjust equipment unless properly trained
Semi-Rigid Isolator Daily Checks and Inspections
Filter Locations
HEPA filter
ExternalFilter
But how do you use it?
Introduction of supplies
Supplies always in unit
Disinfectants Sharps Forceps Scraper Marking Pens Absorbent Towels Paper Bags Hand Broom
Supplies introduced to unit
Feed Bedding Cages Water Sterilized vs
Irradiated
Port Door
Loading the Isolator:
•Remove all jewelry, watches and especially rings•Don protective glove liners•Gather appropriate supplies•Open the outside port door•Place supplies into port –Spray down supplies with disinfectant/sterilent
•Allow sufficient contact time•20 – 40 minutes•Secure outer door
How to Use the Semi-Rigid Isolator Units
Loading the Isolator: continued
•Push the sleeves into the isolator by holding onto cuff•Over stretching the gloves will cause damage •Open the port door •Remove items from port into the isolator•After all items have been removed, shut and lock port door clamps
How to Use the Semi-Rigid Isolator Units
Unloading the Isolator:
•Once everything is ready to be removed from the isolator spray the inside of the port completely•Spray all items before placing them into port•Once all items are sprayed down with Clidox the port can be loaded•After everything is loaded the port is then closed•Set the timer according to SOP/Chemical Manufacturer
How to Use the Semi-Rigid Isolator Units
Unloading the Isolator:
•When the timer goes off you may unload the port•Contaminated items should be disposed/sanitized appropriately.
How to Use the Semi-Rigid Isolator Units
How to Use the Semi-Rigid Isolator Units
Label door with time initials
Set exposure timer
Document event in log
Introducing/Removing Animals to Isolator
Supplies Biosafety Cabinet Plastic bottle/containers
Nalgene® Disinfectant Forceps
Introducing/Removing Animals to Isolator
Transfer into bottle/container inside BSC Leave lids unsealed until all containers are filled
Use same introduction procedures as supplies Ensure animals are promptly removed from
bottle/container Air supply in sealed container must be established
Removing Animals from Isolator
Return to plastic bottles/containers
Double wrap in bag Paper or plastic?
Use regular “spray out” procedures
Air supply for animals limited!
Inside of the isolator check•Shelving and all surfaces for cracks or cuts in the plastic•Light bulb and casing•Port and clamps to ensure that it seals inside and out •If any one of these items are wrong or not working report immediately•DO NOT leave trash inside the isolator•DO NOT leave the isolator dirty
Animal checks inside the isolators•Check feed and water levels in each cage•Observe the animals to ensure health status•Check for and change any wet cages
Semi-Rigid Isolator Daily Checks and
Inspections
Cage changing•Before cage changing begins ensure that all of the daily checks have been completed•Make sure all items for changing are inside the isolator before starting, this will help with being efficient•Change cages•After the cages have been changed remove all trash and dirty bedding from the isolator•Make sure all animals are present and safely in their home cages•Wash down the inside of the isolator, all surfaces including the inside of the glove sleeves•Once all trash is removed wipe down the port
Filter checks•Check the outside filter for any dirt, debris, or damage•Report to a supervisor if something is found
Semi-Rigid Isolator weekly Checks and Inspections
SRI Supply Checklist
Isolator # 1 2 3 4 5 6 7 8 9
Egg Timer
Thermo hygrometer
Brush
Scraper
Wescodyne (diluted)
Pen/Sharpie
Paper Bags
Forceps
Towels
Water Bottles/Sipper Tops
Cage Bottoms
Wire bar lids
Card Holders/ Cage Cards
TP Rolls and Nestlets
Sharps Container
Date Animals Enter
Investigator Name
Date Animals Removed
Date Isolator Deconned
Initials
ISOLATOR # _______