Semi-preparataive purification of native and chemically ... · v!"#$$ !%#$$ !&#$$ ’!#$$ ’"#$$’(#$$)+,-./ $#$0 $#$1 $#$2 $#!$ $#$’ $#$$ 34!"# $%# &"# !%# $’(#) z 2#$$ !’#$$

!"#$%&'(")*'+,"-...

/

!"#$%&'"&('()$*"+&,'$-$.()$/0+/-+0()$*"+(01.2"#$.(334+#/1$-$"1+/3$5/1"/64'$7/%

0,.3"/)$1"+&'/7"8+74+'"*"'8"1%&2(8"+9:;<+=$)2+>!?+@!+1")".)$/0

0$'%12"314$'"56"78*9$(":,"#8&$%6")8'1(")8;9($&6":<$"=;>9$26":>*;$'>"?,@,"=*;<1&'

!"#$%&'()%*+,'-.'/"*0$'1#+'/203)%4,'/5'67898

A@B)"-...6"C;2&"-DEF.6"?&$%%4&

!"#$%&'(")*'+,"-...

-

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

V78)'(.)

!"#$%&'(")*'+,"-...

F

F=/+#(U/'+#")2/18+('"+,8"1+-/'+&,'$-$.()$/0/-+WXV+/3$5/0,.3"/)$1"8Y

A@B)G"HII&'("J**>"(&+$'$%1*2";+"%*"KF."9&')*2<&21&2%"I'$L%1*2"L*44&L%1*2,:$(14M"(L$4&$N4&"I'*9"$2$4M%1L$4"%*"(&91E+'&+$'$%1<&"(L$4&,

?4$N"J&4"&4&L%'*+8*'&(1(G"?;+&'1*'"(&+$'$%1*2"+*O&'";+"%*"K/.."9&'P1II1L;4%"N$2>"<1(;$41Q$%1*2"$2>"I'$L%1*2"L*44&L%1*2,78&"%&L821R;&"1("2*%"&$(14M"(L$4&$N4&"$2>"$;%*9$%$N4&,

Z(.[5'/,01

!"#$%&'(")*'+,"-...

D

:'(.)$.(3+(8&".)8+/-+WXV+8"&('()$/0+74+9:;<+Y

S "=*%8"1*2E&TL8$2J&"$2>"1*2E+$1'"'&<&'(&>"+8$(&"A@B)"*II&'""(1914$'(&+$'$%1*2"+&'I*'9$2L&,

S "?&+$'$%1*2"(&4&L%1<1%M">&L'&$(&("O1%8"*41J*2;L4&*%1>&"4&2J%8"U(&&"+$2&4"VWX,

S "Y*2E+$1'"Z@EA@B)";(&("<*4$%14&"N;II&'(,"[*"(;N(&R;&2%">&($4%12J"*I"%8&($9+4&"1("'&R;1'&>,"Y*2E+$1'"Z@EA@B)"1("9*'&"L*9+$%1N4&"O1%8"0?>&%&L%1*2,

S "3&2&'1L"(8$44*O"J'$>1&2%"1(";(&>\"121%1$4"J'$>1&2%"(%'&2J%8"1("*+%191Q&>,

S "78&";(&"*I"&4&<$%&>"%&9+&'$%;'&6"(4*O"9*N14&"+8$(&"I4*O"'$%&6"$2>"(9$44+$'%1L4&"(1Q&"(*'N&2%"U2*2E+*'*;("+$'%1L4&(X"19+'*<&"(&+$'$%1*2"+&'I*'9$2L&,

S "@*'*;("-,V"]9"+$'%1L4&("*II&'"$"J**>"L*9+'*91(&"N&%O&&2"L*4;92"4*$>12JL$+$L1%M"$2>"L8'*9$%*J'$+81L"&II1L1&2LM,"?&91E+'&+$'$%1<&"+;'1I1L$%1*2"1(+*((1N4&";(12J"$2$4M%1L$4"L*4;92("U+$2&4"^X,

Z(.[5'/,01+B</0)ID

!"#$%&'(")*'+,"-...

V

!"#$$ !%#$$ !&#$$ '!#$$ '"#$$'(#$$)*+,-./

$#$0

$#$1

$#$2

$#!$

$#$'

$#$$

34

!"#

$%#

!%#

&"#

$'(#)*

Z

2#$$ !'#$$ !0#$$ '$#$$ '2#$$'1#$$)*+,-./

$#$0

$#$1

$#$2

$#!$

$#$'

$#$$

34

+#

,#

&&#

&%#

&!#

&$# &-#

&"#

!%#

!"#

$%#

V

</#&('$8/0+/-+8"&('()$/0+/-+WXV+2/#//3$5/0,.3"/)$1"8+74YBVD+%+C:%9:;<\+B1FDL%]TE+BZD+%+<^>\++B1FDKO%]T

_V"T"D,`"996"-,V"]9a7&''$70"0?")/^6"F`"912J'$>1&2%"I'*9"/."%*"/D,V"b$L&%*21%'14&"12".,/"07:WWL6"+A"_6.,V"94c9126"V/,Vd)6ef"-VV"29

F/c-D"L96""_V"]9"L$+144$'M/F"b"412&$'"+*4M$L'M4$91>&12"_0";'&$6"/Vb"I*'9$91>&.,/"0"7'1(E7W@?6"+A"^,F6/V"gf"';26"F.d)ef"-_."29

!"#$%&'(")*'+,"-...

/

!""#$%&'$()*(+*,-.//&0*12*345*%(#67)+(/*8."&/&'$()*(+*9:!*(#$;()6%#.('$<.8

01"2$(%"3*4%'*5"*6"(74%8&%93"*59:*4;35&*%9<&("+;'9%7"=+$4&5">1,

-1"?&@9A+'&+$'$%9B&"+;'9693$%9*4"*6"<&%'9%75$%&<"*59:*4;35&*%9<&(=+$4&5"C1,

D1"E9:8"69<&59%7"+;'9693$%9*4"*6"(+&39$5"*59:*4;35&*%9<&(F"&,:,65;*'&(3&4%57"5$G&5&<"+'9@&'("=+$4&5"0H1F"$4<"I$JK$4L+'*G&("=+$4&5"0/1,

M1"N?OAK?"9<&4%9693$%9*4"*6"*59:*4;35&*%9<&"9@+;'9%9&(=+$4&5("0DF"0MF"0HF"$4<"0/1,

!"#$%&'(")*'+,"-...

_

_(8)+#/0$)/'$05+/-+840)2")$.+WXV/3$5/0,.3"/)$1"+&,'$)4I

V."T"D,`"996"-,V"]9"a7&''$70"0?")/^6J'$>1&2%"I'*9"_,V"b"$L&%*21%'14&"12".,/"07:WWL6"+A"_6"%*"/."b"$L&%*21%'14&"12.,/"0"7:WWL6"+A"_6"12"-."912;%&(.,V"94c9126"V/,V"d)6"ef"-VV"29

012;%&( 012;%&(

ef

-VE9&'

. /^j . /-`

-DE9&'

/VE9&'

/DE9&'!"5.6"VkE"WWW"WWW"WWW"WWW"WWW"E"Fk

'"75.6""VkE")7)"7)3")W)"))W"7)7")7)"7))"77)"7"E"Fk

!"#$%&'(")*'+,"-...

^

A@B)"L*2>1%1*2(G"_V"T"D,`"996"-,V"]9"a7&''$70"0?")/^6"WG".,/"0"7:WWL6"+A"_\"=G"-V"b"W)[12".,/"0"7:WWL6"+A"_\"J'$>1&2%"I'*9"D."b"="%*"VV"b"="12"F."9126".,V"94c9126"V.,Vd)6"ef"-`."29

?&91E+'&+$'$%1<&"1*2E+$1'"Z@E"A@B)+;'1I1L$%1*2"*I"F.E9&'"*41J*7

)*44&L%1*2(%$'%

?%*+

)=W

!(#&3" ?0U".)"1 ?0U".)"1+#(88

+*/3,#"+Bµ;D B0#/3D B#5D

V O KT TIT`K

Z KO ]T TIRNR

< PO `T TILKQ

</33".)"1+-'(.)$/0:,'$)4+BaD

`]IR

`QIP

`QI]

ef"-`."29

. """""""""""/. """"""-. " """F.U012;%&(X

.

/

-

F

D

@;'1I1&>"F.7""""Uj`,D"bX

-j7

-^7

!"#$%&'(")*'+,"-...

j

"78&"'&%&2%1*2"%19&"*I"P[W"I'$J9&2%">&+&2>("*2"1%("4&2J%8"$2>"(&R;&2L&6"%8&'&I*'&"$9$2;$4"I'$L%1*2"L*44&L%1*2"1(";(;$44M"+&'I*'9&>,

S "0*4&L;4$'"9$(("1("$2";2$9N1J;*;("L'1%&'1*2"I*'"L*44&L%1*2"*I">&(1'&>"I'$J9&2%,"#&;(&"0?">&%&L%1*2"$("$"%'1JJ&'12J"&<&2%"I*'"I'$L%1*2"L*44&L%1*2,

S "78&".,/"0"L*2L&2%'$%1*2"*I"%'1&%8M4$912&"$L&%$%&"U7:WWLX"1*2E+$1'"N;II&'"J1<&(";($"J**>"Z@EA@B)"(&+$'$%1*2"*I"*41J*2;L4&*%1>&(6"N;%"(;++'&((&("%8&"(&2(1%1<1%M"*I:?YE0?"U+$2&4"//X,"B*O&'"L*2L&2%'$%1*2"*I"7:WWL"L*9+'*91(&("%8&"A@B)(&+$'$%1*2"UW+II&4"W,"&%"$4,6"C,")8'*9$%*J',"W6"/jj_6"___6"F\"A;N&'"),3,6"l'$m&%&"W,6W2$4,)8&9,"/jjj6"_/6"F_F.X,

S "A&T$I4;*'*1(*+'*+$2*4"UAhY@X"E"%'1&%8M4$912&"N;II&'"O$("+'*+*(&>"12(%&$>"*I%'1&%8M4$912&"$L&%$%&"UW+II&4"W,"&%"$4,6"W2$4,")8&9,"/jj_6"`j6"/F-.X,"?&+$'$%1*2+&'I*'9$2L&"*I"AhY@E7:W"N;II&'">*&("2*%"I;44M"9$%L8"%8&"+&'I*'9$2L&"*I"7:WWL,

S "#&"*+%191Q&>"%8&"1*2E+$1'"N;II&'"%*"19+'*<&"%8&"(&+$'$%1*2"*I"*41J*2;L4&*%1>&(O814&"g&&+12J"9*N14&"+8$(&">1'&L%4M"L*9+$%1N4&"O1%8":?YE0?">&%&L%1*2,

9:;<+#")2/1+1"*"3/&#"0)+-/'+8"#$%&'"&('()$*"+&,'$-$.()$/0+/-+WXV

/3$5/0,.3"/)$1"8+7(8"1+/0+>!?%@!+1")".)$/0

!"#$%&'(")*'+,"-...

/.

b&)$#$M()$/0+/-+F>V%9_?:+7,--"'

ef"-`."29

. """""/. """"""""""-. """""""""""""""F.012;%&(

/.7/V7

F.7

/.7

/V7

89/.."90"AhY@6"

^,`"90"7:W6"+A"^,-V

39/.."90"AhY@6"

.,V`"90"7:W6"+A"_

S" D.."90"AhY@E-,-V"90"7:W"N;II&'6" +A"_" UW+II&4" $%" $4,6" W2$4,")8&9," /jj_6" `j6" /F-.X+&'I*'9(" +**'4M" I*'" 4*2J" *41J*2;L4&*%1>&(" Un" F."9&'X,"0*'&" +'*2*;2L&>" +&$g" N'*$>&212JO$(" *N(&'<&>" O1%8" 9*N14&" +8$(&" L*2%$1212J" /.." 90" AhY@6" .,V`" 90" 7:W6" +A" _" N;II&'UL8'*9$%*J'$9" W6" (&+$'$%1*2" *I" *41J*>&*TM%8M91>12&" *41J*2;L4&*%1>&" 4$>>&'" ÊF.9&'" 76a7&''$i"0?")/^"_VTD,`996"-,V"]96"J'$>1&2%"(%$'%("I'*9"/."b"0&HA6"(4*+&".,D"b"0&HA+&'"912;%&6".,V"94c9126"L*4;92"%&9+&'$%;'&"V.d)6"-`."29X,

S" )*2L&2%'$%1*2" *I" 7:W" 12" N;II&'" 1(" L';L1$4" I*'" L8'*9$%*J'$+81L" +&'I*'9$2L&," #&12<&(%1J$%&>" %8&" &II&L%" *I" 7:W" L*2L&2%'$%1*2" U.,VÈF/,D" 90\" +A" _EjX" g&&+12J" %8&" AhY@L*2L&2%'$%1*2"L*2(%$2%"U/.."90X,"78&"/.."90"AhY@E^,`"90"7:W"N;II&'6"+A"^,-V"J$<&";($2"*+%19$4"(&+$'$%1*2"UL8'*9$%*J'$9"=6"L*4;92"$2>"A@B)"L*2>1%1*2("%8&"($9&"$("WX,

S"a7&''$i"0?")/^"L*4;92" U8MN'1>"+$'%1L4&" %&L82*4*JMX" %*4&'$%&(" %8&" &4&<$%&>"+A"O1%8*;%2*%1L&$N4&">&%&'1*'$%1*2"*I"L*4;92"+&'I*'9$2L&"*<&'"(&<&'$4"8;2>'&>"12m&L%1*2(,

!"#$%&'(")*'+,"-...

//

"%./.012.3.456*.&%%7).84.,.$%.9:;<.&%%1.=<>:?@.#

PTT OTT QTT NTT LTT `TT KTTT KKTT KRTT K]TT KPTT KOTT KQTT KNTT KLTT K`TT RTTT RKTT RRTT#cMT

KTT

a

T

KTT

a

T

KTT

a

T

KTT

a

(RO&8+2-$&KTT+`++O+BTIKTKD+!#+B@0E+R6]ITTD\+<#+BRYOTD Fb_+@!+>!%+RILQ"P

(RO&8+2-$&KTT+LdRQ++K+BTITR]D+!#+B@0E+R6]ITTD\+<#+BKYOTD Fb_+@!+>!%+RILQ"P

(RO&8+2-$&KTT+N++R+BTITPOD+!#+B@0E+R6]ITTD\+<#+BRYOTD Fb_+@!+>!%+RILQ"P

(RO&8+)"((.++]+BTITQRD+!#+B@0E+R6]ITTD\+<#+BRYOTD Fb_+@!+>!%+RILQ"P

>--".)+/-+7,--"'+/0+>!?%@!+8$50(3+/-+RO%#"'+WXVB)76"01L'*9$((6">1'&L%"12I;(1*2"12"V."b"W)[EN;II&'

&4&L%'*(+'$M"L$+144$'M"-,V"gf6"($9+4&"L*2&"F."f6"/..d)">&(*4<,6"^.d)"(*;'L&6">&(*4<$%1*2"J$("D^."4c8*;'

/.."90"AhY@E"F/,D"90"7:W6"+A"j

/.."90"AhY@"E"^,`"90"7:W6"+A"^,-V

/.."90"AhY@"E".,V`"90"7:W6"+A"_

/.."90"7:W"E"/.."90"$L&%1L"$L1>6"+A"_

17"707

%727

&7!$7

!!7

!'7

!(7

!"#$%&'(")*'+,"-...

/-

!"#$#%&'#(#)*+,#-""./#0)#1#2!#3456#-7"&#8694:;#<

!"" #"" $"" %"" &"" '"" (""" (("" ()"" (*"" (!"" (#"" ($"" (%"" (&"" ('"" )""" )("" ))""+,-"

(""

.

"

(""

.

"

(""

.

/)#0123450(""2'22*26"7"$)829+26:;<2)=*7""8>2?+26)@#"8 ABC2:92D9E2)7((F!

G)#0123450(""2'22!%26"7'!(829+26:;<2)=*7""8>2?+26)@#"8 ABC2:92D9E2)7((F!

H)#0123450(""2'22#26"7("(829+26:;<2)=*7""8>2?+26)@#"8 ABC2:92D9E2)7((F!

)*0&"1234"5&(*67$%8*0"/9.:)

)*0&"1234"5&(*67$%8*0"/..:)

)*0&"1.34"5&(*67$%8*0"/..:)

!

"

#

;<="$55>?%(

$%

&%'%(%)%

*%+,%

++%

D44F/I2J42D9KE:92/J;L5I5J;12J;2HGM;LH;/FJ42)#E+FN2OPQ2E2ADQ2HLLM/I1

@);4"A8?'*B$((4"58'&?%"80C>(8*0"80"2."D"=)EFG>CC&'4"/.."BA"HIJKF1/,L"BA";<=4"+H"M?$+866$'N"-,2"O34"(*>'?&"9.:)4"5&(*67$%8*0"P$("L9."6QR*>'4"C*'"($B+6&"?*0&"7*6%$P&"$05"5&(*67$%8*0

%&B+&'$%>'&"(&&"$00*%$%8*0

!"#$%&'(")*'+,"-...

/F

)$+144$'M"B)EB)7"(M(%&96"L*4;92"V.T/"996"a7&''$i"0?")/^6"-,V"]9\"9*N14&"+8$(&"WG"/.."90E"^,`"90"7:W6"+A^,-V6"=G"9&%8$2*4\"J'$>1&2%"(%$'%("$%"/."b"=6".,D"b"="+&'"912;%&6"I4*O"'$%&"-F,`"]4c9126">&%&L%1*2"@PW"$2>":?YE0?6

V.d)6"&4&L%'*(+'$M"L*2>1%1*2("(&&"+$2&4"/-")6"0?"(+&L%'$"(&&"+$2&4"/D

>!?+@!+.2'/#()/5'(#+/-+2/#//3$5/%1"/64)24#$1$0"+3(11"'+8"&('()$/0

?&4&L%&>":?YE0?"(+&L%'$"(&&"+$2&4"/D

KTITT RTITT ]TITT@$0,)"8

T

KTT

a

Fb_+@!+>!%+

F?<+KITT"O

U>7XF.

U>7X//

@8*(+8*'M4$%&>+U>7X/-

!"#$%&'(")*'+,"-...

/D

F.9&'"7

Fb_+@!+>!%+KIQK"]

OTT KTTT KOTT RTTT ROTT ]TTT#cM

Fb_+@!+>!%+

RP]

?1"0)$-$.()$/0+/-+&"([8+-'/#+9:;<h*'"L8'*9$%*J'$9"(&&"+$2&4"/F6":?YE0?"L*2>1%1*2("$'&"%8&"($9&"$("*2"+$2&4"/-)

/-9&'"7+8*(+8*'M4$%&>

//9&'"7

Fb_+@!+>!%+

RQQ

'7(7

17"7

'7

(7

;<39=>>,?-/

!"#$%&'(")*'+,"-...

/V

?#&,'$)$"8+$1"0)$-$.()$/0+$0+RO#"'+-3,/'"8."$03(7"3"1+&'/7"+74+;<%ef%@!

V.T/99"a7&''$i"0?")/^6"-,V"]96"J'$>1&2%"(%$'%("I'*9"/V"b"0&HA6"(4*+&".,V"b"0&HA"+&'"912;%&6"-F,`""]4c9126"L*4;92"%&9+&'$%;'&"V.d)6"ef"$2>":?Y"0?">&%&L%1*26"0?"L*2>1%1*2"(&&"+$2&4"/-")

?$9+4&G"Vk"E"UhB?[X"3W)"77W"3W)"77W"3W)"77W"3"E"Fk

RY+W$/1"+V''(4+OTT

]ITR"O

RY+W$/1"+V''(4+RQT

KIRT"Q

KY+Fb_+@!+>!%+F?<

QIO]"P

TITT ]ITT QITT ÌTT KRITT KOITT KLITT

KTT

a

KTT

a

KTT

a

?8*'%"I$14;'&"(&R;&2L&(

hB?["1(*9&'"/

hB?[E[A-""1(*9&'"/

hB?["1(*9&'"- hB?[E[A-""1(*9&'"-

h;44"4&2J%8""*41J*2;L4&*%1>&"1(*9&'("o"19+;'1%1&(

-"T"hB?[ ef"V.."29h4;*'&(L&12">&%&L%1*2

ef"-`."29W44"P[W">&%&L%1*2

:?Y"0?"7*%$4"1*2"L*;2%

!"#$%&'(")*'+,"-...

/0

!"#$%&'"(&")&"**+,-./0!(

!"## $"## %&"## %'"## &#"## &!"## &$"##()*+

%##

,

-%

%##

,

%##

,

&./0)12+/34456/77'

8"'#+7

&./0)12+/34456/7##

&"!%+7

%./(9:/;</=<-/(>?

$"$8+!

12*'%"3$456'&(&76&89&(

:$456'&"(&76&89&(5$;&5&<"=4%2"&4%2&'">?@:A1B"*'"C?@DEFGE

:655"5&8H%2"+'*<69%I&(4'&<"3'$9%4*8J

-"@"K"L&'"3$456'&"(&76&89&("5$;&5&<"=4%2"C?@DEFGE

MN">>0"8LDEFGE"<&%&9%4*8

MN">.."8L:56*'&(9&48"<&%&9%4*8

O1P"F1"D*%$5"4*8"9*68%

>*@A4)B)+C/)2+DB)E)F5B)1D/)D/(5G;5D/&%-*+4@41H+/H6/I?-JK-;</1E

>.Q/LL"RD&''$"F1")/ST"-,>"ULT"H'$<4&8%"(%$'%("3'*L"/>"V"F&WXT"(5*+&"/"V"F&WX"+&'"L486%&T"-C,0L5YL48T"9*56L8"%&L+&'$%6'&">.Z)T"MN"$8<"O1P"F1"<&%&9%4*8T"F1"9*8<4%4*8"(&&"+$8&5"/-")

1$L+5&[>?"@"\:A1B]"@")E))D))E^D^ÊEED)EE^D"@\DEFGE]"@"C?

!"#$%&'(")*'+,"-...

/_

</0.3,8$/0

&F.G#<AAHI.)J.1&+.9:?K7;.D>.K><CK?.C:A.L20:?DM:;K9?<:ND=<.OKH?DNP.9:;NA:?E

!F.J<7D(8A<8HAHND@<.L20.8KADCD9HND:;.QH>=<7:;>NAHN<=E

$F.R8ND7DS<=.T1.7:UD?<.8BH><.:CC<A>.U:NB.M::=4*T1.><8HAHND:;.:C.:?DM:;K9?<:ND=<>.H;=9:78HNDUD?DNP.QDNB.VJ6.)J.=<N<9ND:;E

-F.0KN:7HN<=.4*T1.CAH9ND:;.9:??<9ND:;.QH>=<>DM;<=.UH><=.:;.)J.=<N<9ND:;.:C.><?<9N<=7:?<9K?HA.7H>>.:C.=<>DA<=.L20.CAHM7<;NE

Documents

Semi-preparataive purification of native and chemically ... · v!"#$$ !%#$$ !&#$$ ’!#$$ ’"#$$’(#$$)*+,-./ $#$0 $#$1 $#$2 $#!$ $#$’ $#$$ 34!"# $%# &"# !%# $’(#)* z 2#$$ !’#$$

Semi-preparataive purification of native and chemically ... · v!"#$$ !%#$$ !&#$$ ’!#$$ ’"#$$’(#$$)+,-./ $#$0 $#$1 $#$2 $#!$ $#$’ $#$$ 34!"# $%# &"# !%# $’(#) z 2#$$ !’#$$