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7/27/2019 Secrets of SPE
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The Secrets of Solid Phase Extraction (SPE)
for Sample Preparation
Agilent AccuBONDII, AccuBONDII ENV and EVIDEXIISPE
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CI0126C 2
What Is Solid-Phase Extraction (SPE)?
• Sample preparation technique with principles similar to those of
HPLC for selective adsorption of analytes or interferences from
complex matrices
• Used for sample cleanup and analyte concentration preceding
LC, GC, ion chromatography and other techniques
• Cost-effective alternative to/replacement for liquid-liquidextraction (productivity, solvent, waste)
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CI0126C 3
Advantages of SPE vs. Liquid-Liquid Extraction
• Improved throughput (parallel vs. serial processing)
• Decreased organic solvent usage and waste generation
• Higher and more reproducible recoveries
• Cleaner extracts (contamination, solvent impurities)
• No emulsions
• Tunable selectivity ( SPE phase choices, solvent mixtures)
• Readily automated
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CI0126C 4
Typical Applications of SPE
• Sample Cleanup
– Combinatorial reaction cleanup before LC-MS or LC
– Pharmacokinetic studies, dissolution testing
– Isolate analytes from complex matrices – urine, plasma – Remove “column killers” or major interferences
– Eliminate late-eluters to allow isocratic analysis
• Trace Enrichment
– Environmental analysis – Pharmaceutical and Agrochemical applications
• Desalting
• Solvent Exchange
• Sample Preservation and Storage
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CI0126C 5
Application and Importance of SPE is Growing
…Rapidly• Applied in cartridge format for 20+ years
—Critically important for sample cleanup and trace enrichment
(esp. environmental and pharmaceutical labs)• SPE usage is accelerating
—Combinatorial synthesis and high-throughput LC-MS analysis
—Overall LC-MS usage increases and greater instrument uptime
—Smaller samples requiring smaller bed masses (tubes and plates)
—Minimization of organic solvents and waste
—Ease of automation (cost, reproducibility, throughput)
• Liquid handling and SPE workstations• 96-well plate autosamplers
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CI0126C 6
Typical SPE Formats
Schematic Diagram of a 96-Well SPEExtraction Plate System*
* 3M Corp.
Manifo ld System
Single
Extraction
Disk
SPE Cartridge
Schematic Diagramof a
96-Well Plate Extraction System
(LC-GC, S9, May, 1998)
R. E. Majors, LC-GC, 15(4), 318 (1997)
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CI0126C 7
SPE Modes—”Digital Chromatography”
Matrix Adsorption
• Analyte(s) unretained (k ~ 0)
• Matrix retained(k >> 1)
• No preconcentration advantage
• Eluates may not be as clean
• Sample loading often gravity fed
• Used less often than analyteadsorption
Analyte Adsorption
• Analyte(s) retained (k >> 1)
• Matrix unretained(k ~ 0)
and/or strongly retained (k >> 1)
• Preconcentration factor
• Cleaner extracts
• Load at 1-3 drops/sec (recovery ∝1/flow)
• Capacity issues may be moreimportant
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CI0126C 8
Fundamental Steps for SPE Adsorption Mode
Prewash*
Remove contaminants that
could elute with analyte
Precondition Load Wash Elute
Prepare cartridge to
accept sample
Load sample and
rinse reservoir (s)
Wash with solvent that
won’ t elute analyte
Elute analyte in smallest
volume possible
If elution solventwill be stronger
than precond. solvent
1. MeOH or ACN
2. Weak solvent(water, buffer )
Weakly retainedmatrix compds elute
Analyte and other
matrix compds
retained
Elute analyte
leaving highly
retained compds
1 2
* N o t n e c
e s s a
r y
f o r A g
i l e n t S P
E
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CI0126C 9
General SPE Method Development Strategy
SPE Adsorption Mode: Background
• Research the Problem
—Previous SPE and analysis conditions for the analyte and matrix?
• Characterize the Analyte
—Structure, pKa, polarity, functional groups
—Solvent solubility and stability
• Any restrictions on final solvent and concentration(technique or instrument)?
• Characterize the Sample Matrix
—Possible interferences — similar functional groups, pKa, etc.
—pH, ionic strength —Solvent solubility and stability
—Qualitative and quantitative variability
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CI0126C 10
General SPE Method Development Strategy
SPE Adsorption Mode: Experimental
• Develop or apply effective HPLC or GC conditions to monitor progress
— Assess recovery and eluate cleanliness
• Select and test sorbents
—Determine which sorbents provide maximum analyte retention
—Determine which eluent solvents yield highest recoveries
• Identify optimum wash solvent
—Assess eluate cleanliness under conditions of maximum analyteretention
—Determine strongest wash solvent that will not elute analyte
• Test blank and fortified matrix
—Assess eluate cleanliness and recovery using optimum wash and eluentsolvents
• Test real samples and fortified samples
—Assess eluate cleanliness and recovery for different unfortified andfortified samples
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CI0126C 11
SPE Method Validation
Variables to Consider
• Validate SPE procedure
—Sorbent (sorbent weight, different cartridges, different lots)
—Preconditioning (strong solvent, weak solvent)
—Loading solvent (% organic, pH, ionic strength, volume)
—Wash solvent (% organic, pH, ionic strength, volume)
• Eluent (volume, % organic …) —Flow rates (loading, wash, elution)
—Linearity and range (different analyte concs. and matrix loadings)
—Analyte stability (loading solvent, eluent)
—Matrix stability (loading solvent)
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CI0126C 12
Design Characteristics for SPE Family
• Provide a variety of popular chemistries to meet most
application needs
• Provide silica and PS-DVB base materials
• Provide most popular sizes of cartridges and bulk material for
“self-packers”
• Pre-clean packing, tubes and frits – low organic extractables for high sensitivity work
• Economical for routine applications
• Excellent quality assurance program to ensure consistentresults from batch-to-batch and year-to-year
• Specialty phase for drugs of abuse testing
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CI0126C 13
Break Time
For Questions and Answers
Press *1 on Your Phone to
Ask a Question
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CI0126C 14
SPE Products from Agilent
AccuBONDII
and EVIDEXII
• Improved AccuBONDII SPE Products
—Solid Phase Extraction Cartridges —Bulk SPE Adsorbents and Accessories
—Method Development Kits
—Applications
• EVIDEXII SPE Specialty Cartridges
—Drugs of Abuse
—General Pharmaceutical Extractions
—Applications
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CI0126C 15
AccuBONDII SPE Phases
Sulfonic acidStrong Cation Exchange
(SCX)
Quaternary amineStrong Anion Exchange
(SAX)
Sil ica, -CN, Diol, Amino,
Alumina (acidic, basic, andneutral), Florisil
Normal Phase (polar)
C2, C8, C18, Phenyl,ENV PS-DVB*
Reversed Phase
Phases AvailableSPE Mode
* Newest phase
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CI0126C 16
AccuBONDII SPE Cartridges
• Silica and new PS-DVB cartridges available
• Wide variety of cartridge sizes
—100, 200, 500 and 1000 mg
—1, 3, and 6 mL tubes —96 well plates in final development
—Tabless cartridges for automation (i.e. Gilson)
• Excellent quality assurance program
—Random testing of cartridges
• surface characteristics
• packing parameters
—Performance certificate in each box• Low extractables
—Pre-washed packings, frits, and tubes
• Bulk packings available – 25 g bottles
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CI0126C 17
AccuBONDII SPE – Low Levels of Extractables
Tube and Frit
min0 4 8 12 16
min0 4 8 12 16
Agilent SPE AccuBONDII
Pre-washed Tube/Frit Only
Waters’ Sep-Pak C18
Tube/Frit Only
Programmed Temperature
GC-FID Analysis
Splitless Injection
Internal Standard
Total = 50 ppm
Total = 5 ppm
Internal Standard
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CI0126C 18
AccuBONDII SPE Method Development Kits
• SPE Method Development Kits – groups of phases thatseparate using the same mechanism
—Kit A (Reversed Phase)• C2, CN, Phenyl, C8 and C18
—Kit B (Normal Phase)
• Si, Amino, Diol and CN —Kit C (Ion Exchange)
• SCX, SAX and Amino
• Multiple kits provide a wide variety of options for determiningthe best mechanism and phase type for your analyte andmatrix
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CI0126C 19
AccuBONDII SPE Application Notes
• Application Notes for AccuBONDII
—Environmental
• Extraction of Chlorinated Pesticides in Water (SE-1)
• Extraction of Polynuclear Aromatic Hydrocarbons in Water (SE-2)• Extraction of PCB’s in Water (SE-3)
• Extraction of Triazines Using SPE Cartridges
—Pharmaceutical
• Anabolic Steroids in Urine and Serum (SC-1)
• Benzodiazepines in Serum (SC-2)
• Caffeine and Metabolites in Serum (SC-3)
• Barbiturates in Serum (SC-4) —Available online:
www.agilent.com/chem
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CI0126C 20
AccuBONDII Example Applications
Triazines in Different Matrices
• Characterize the Analyte
• Structure, pKa, polarity,functional groups
• Solvent solubility andstability
• Any restrictions on finalsolvent and concentrationdue to technique or
instrument?
Triazines
• Three major species – simazine,atrazine and propazine – all
structurally similar
• Mode of Action: Herbicides
• Practically insoluble in water
• Soil – large number of chargedspecies-adjust pH to retain triazinesand do ion-exchange
• Muscle tissue – large amounts of non-
polar lipids – retain these and elutetriazines using C18
• Corn oil – non-polar glycerides andfatty acids –weakly retained on diolwhile triazines are strongly retained
• Characterize the Sample Matrix
• Solvent solubility and stability
• pH, ionic strength
• Possible interferences—similar
functional groups, pKa, etc.
• Qualitative and quantitative
variability
N N
N
Cl
NH NH
R1R2
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CI0126C 21
SPE Methods for Triazines in Complex Matrices
MethanolMethanol Acetonitrile/K2HPO4ELUTE
HexaneWater Acetic acid,acetonitri le, water,
0.1 M K2HPO4
WASH
Diluted with hexaneDiluted with water Diluted with acetic
acidLOAD
Methanol, hexaneMethanol Acetic acidPRE-TREAT
NoneHomogenized in
methanol
Shaken in
acetonitrileEXTRACTION
DiolODSSCXCARTRIDGE
Corn OilMuscle TissueSoil
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CI0126C 22
HPLC Analysis of Triazines Extracted
from Different Matrices
Triazines from Corn Oil
with Diol
Triazines from Muscle Tissue
with ODS
Triazines from Soil
with SCX
Column: C18, 4.6 x 150 mm, 5 mm Mobile Phase: 50% methanol:50% 0.01M K2HPO4 Flow Rate: 2 mL/min
Detection: UV 254 nm Sample: Triazines 1. Simazine 2. Atrazine 3. Propazine
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CI0126C 23
AccuBONDII Example Applications
Barbiturates in Serum
• Characterize the Analyte
• Structure, pKa, polarity,functional groups
• Solvent solubility andstability
• Any restrictions on finalsolvent and concentration
due to technique or instrument?
Barbiturates
• Non-polar side groups make retention
on C18 possible – but retention notstrong
• Mid pH will eliminate charge andimprove retention
• Soluble in water
• Final solvent easy to evaporate or compatible with HPLC
• Serum has many componentscompeting for retention
• Serum will vary from person to person
• Characterize the Sample Matrix
• Solvent solubility and stability
• pH, ionic strength
• Possible interferences—similar functional groups, pKa, etc.
• Qualitative and quantitative
variability
N
NH
O
O
O
R1
R2
R3
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CI0126C 24
Analysis of Barbiturates in Serum
AccuBONDII SPE C18 Cartridge
Solid Phase Extraction Method
CARTRIDGE: AccuBONDII ODS (C18) 6 mL/500 mg
(P/N 188-1356 30/box)
Sample Preparation :
Add 1 mL 0.5M K2HPO4 to 4 mL of serum
Precondition:
5 mL Acetone
5 mL Deionized Water
Load:
5 mL Sample (see sample preparationabove)
Wash
2.0 mL 95% water:5% acetonitrile
Elute:
3.0 mL AcetoneEvaporate and Reconstitute:
Evaporate Acetone in a nitrogen stream at<45°C
Add 200 µL of acetonitrile:water (20/80)
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CI0126C 25
Break Time
For Questions and Answers
Press *1 on Your Phone to
Ask a Question
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CI0126C 26
HPLC Analysis of Barbiturates Extracted
from Human Serum
Column: C18, 4.6 x 150 mm, 5 mmMobile Phase: Time ACN Water
0 20 80
5 20 80
15 40 60
20 40 60
Flow Rate: 1 mL/min
Detection: UV 235 nmSample: Barbiturates
1. Barbital
2. Allobarbital
3. Aprobarbital4. Phenobarbital
5. Butabarbital
6. Alphenal
7. Hexobarbital
8. Amobarbital
9. Mephobarbital10. Secobarbital
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A BONDII SPE P d t F t
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CI0126C 31
AccuBONDII SPE Product Features
COA, Packaging and Labeling
• Certificate of analysis with each box —Mass consistency —Flow rate consistency
—Trace metal analysis —Extract cleanliness
—QC chromatogram
• Packaged in tr ilayer laminated bag —Excludes air and water —Ensures clean product
• Individually printed tubes and plates
—Easy identification —Versatility for method development
C tifi t f P f f A BONDII 1
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CI0126C 32
Certificate of Performance for AccuBONDII – page 1
Description: AccuBONDII C8 100 mg 1 mL cartridge
Catalog No: 188-0310
Lot No.: AMC18-0X
Run No.: XXXXXXX
This AccuBOND product and sorbent have been manufactured, tested and assembled under the control of an ISO 9001 registered quality system.This AccuBOND SPE product has been subjected to the following QC tests:
Base Silica Characteristics Bonded Silica TestsSurface Area: 546 m2/g Carbon Loading: 13.0%Average Pore Size: 60Å Surface Coverage: 2.5 µmoles/m2Surface pH: 7 Extraction Residue: PassMetal Analysis: Pass Exchange Capacity: N/A
Particle Size Data Packed Cartridge Test
Particle Shape: Irregular Cartridge Flow Resistance: PassAverage Particle Size (µm): 56 Frit Purity Test (GC): PassPercentage of Particles <10 µm: 0.00 Material Weight Check: Pass
Percentage of Particles <25 µm: 1.36Percentage of Particles <90 µm: 4.95
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CI0126C 33
Certificate of Performance for AccuBONDII — page 2
T e s t C o n d it io n a n d R e s u lt s
C o n d i t i o n s :
C o lu m n : 4 .6 x 2 5 0 m m
M o b ile P h a s e : 7 0 /3 0 M e O H /H 2 O
F lo w R a te : 2 .0 m l /m in
S p e c i f i c a t i o n s :
K ’ b ip h e n y l = 3 .5 to 6 .5
K ’ to lu e n e = 1 .5 to 3 .5
K ’ a c e to p h e n o n e = 0 .6 to 1 .4
T f u r a c i l = 0 .3 to 1 .5
R e su l ts f o r L o t A M C 8 X -X XK ’ to lu e n e = 4 .5 P e a k 1 = U r a c il
K ’ d i m e th y la n i l in e = 2 .1 P e a k 2 = A c e to p h e n o n e
K ’ n i t r o b e n z e n e = 1 .0 P e a k 3 = T o lu e n e
T f d im e th y la n i l in e = 1 .4 P e a k 4 = B ip h e n y l
N o t i c e :
M a t er ia l S a f e ty D a ta S h e e ts ( M S D S ) a r e a v a i la b l e a t:
h t t p : //w w w .c h e m .a g i le n t .c o m / s c ri p t s/ ca g _ m s d s s e a r c h . a s p
T h i s p r o d u c t h a s p a s s e d a l l A g i le n t T e c h n o l o g i e s , i n c . q u a l it y c o n t r o l s p e c i fi c a t io n s .
QA
QC
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EVIDEXII SPE Cartridges for Drugs of Abuse
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CI0126C 36
S Ca t dges o ugs o buse
Opiates in Urine
Solid Phase Extraction Method
CARTRIDGE: EVIDEXII 400 mg, 6 mL
(P/N 188-2946, Box of 30)
Precondition:
6 mL methanol 6 mL 0.1 M potassium phosphate (pH 6.0)
Do not let the phase go dry
Load:
Add 3 mL 0.1 M potassium phosphate (pH6.0) to the cartridge
Attach an 8 mL reservoir Add the urine sample
Rinse:
Remove reservoir 3 mL water 3 mL 0.1 M sodium acetate (pH 4.5) 3 mL methanol
Elute:
Place a collection tube beneath cartridge 3 mL methylene chloride/isopropyl alcohol/
NH4OH (78/20/2) Collect the eluate
Codeine30 ng
Morphine30 ng
Codeine
Column: DB-5MS EVDX
Carrier: Helium at 40 cm/sec
Oven: 65 degrees for 1 min, 65-325 degrees at 20 degrees/min
Injector: Splitless, 250°C
Detector: MSD, 300°C transfer line
Clean extracts froma very dirty matrix
Morphine
DOA Recoveries are Reproducible
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CI0126C 37
Recovery Levels (x±%, n = 8)
Level in ng/mL 1000 1750 2500 Amphetamine 76 ±4 74 ±2 96 ±3
Methamphetamine 85 ±1 71 ±3 98 ±3
Level in ng/mL 50 87.5 125PCP 90 ±3 91 ±2 88 ±3
Level in ng/mL 300 525 750Benzoylecgonine 98 ±8 93 ±4 97 ±3
Level in ng/mL 600 1050 1500Codeine 99 ±1 97 ±3 98 ±1Morphine 96 ±2 96 ±3 93 ±4
Level in ng/mL 30 52.5 75THC-COOH 92 ±4 96 ±8 94 ±3
p
Using EVIDEXII SPE Cartridges
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SPE Tips For Improving Recovery and Precision
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CI0126C 40
• General
—Keep cartridges in sealed bags until use.
—Store in zipper-locked bags or in desiccator once opened.
—When using empty reservoirs attached to cartridges, use longdisposable pipets to ensure proper flow from reservoir to cartridge.
—Use stopcocks to adjust/control flow through individual cartridges.
• Use mass balance for all fractions to determine fate of analyte (adsorption
to surfaces, loading effluent, washes, eluate, etc.) during methoddevelopment.
—Residual water can be removed effectively by centrifugation (5000
rpm, 5 min.) compared to drying with vacuum or nitrogen.
—Cartridge capacity for analytes and matrix is typically about 1-3% of cartridge bed weight (ion-exchange not included).
—NEVER allow the cartridge to dry out until the elution step.
SPE Tips For Improving Recovery and Precision
SPE Tips For Improving Recovery and Precision
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CI0126C 41
SPE Tips For Improving Recovery and Precision
• Prewash
—Remove all strong prewash solvent for GC (e.g., dichloromethane,
hexane, ethyl acetate) before preconditioning and loading.
• Precondition —Make sure pH is correct for ion-suppression (acids) or minimal
silanol interactions (bases).
—Leave ~1-2 mm of preconditioning solvent above sorbent bed to
prevent bed from drying.
—Leave ~1/4 to 1/2 of tube volume above sorbent bed when using
empty reservoir above cartridge.
SPE Tips For Impro ing Reco er and Precision
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CI0126C 42
SPE Tips For Improving Recovery and Precision
• Load
—Leave ~1/4 to 1/2 tube volume above sorbent bed when using tube
reservoir above cartridge.
—Use drop wise solvent flow when time/throughput is not a major
concern.
• Wash
—Wipe needles of manifold before elute step to minimize contamination of
eluate.
• Elute
—When choosing eluent, consider ease of evaporation if reconstitution is
needed.
—Allow cartridge/plate to soak with eluent for 0.5 - 1 min. (➚recovery).
—Sometimes several smaller eluent aliquots can improve recovery.
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Extraction and Separation of PAHs from Water
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CI0126C 44
AccuBONDII ODSSolid Phase Extraction Method
CARTRIDGE: AccuBONDII , 500 mg, 6 mL
(P/N 188-1356, 30/box)
Sample Preparation:
Add 2 mL Isopropanol to 20 mL Water Sample
Mix thoroughly
Precondition:
5 mL Methylene Chloride 5 mL Methanol 5 mL Deionized Water
Add ~1 mL Deionized Water to top of bed
Load:
Attach 24-mL sample reservoir to cartridge
Add sample to reservoir. Ensure flow into cartridge with glass pipet. Apply vacuum and keep flow rate <10 mL/min. Slower flow gives
better results.
Wash:
3 mL 50:50 Acetonitrile/Deionized Water
Apply vacuum until 30 sec. after wash has passed through cartridge Centrifuge cartridge at 1000-1500 rpm for 5 min. (removes excess
water)
Elute:
3 mL Methylene Chloride and collect eluent. Evaporate to 50-200 µL. Do not apply heat or smaller PAHs will be
volatilized. Add Methylene Chloride to bring sample to final volume of 200 µL.
Inject 2 µL.
Compound Avg.Recovery
(%)
Std.Dev.
(%)
Naphthalene 80 12
2-Chlor onaphthalene 78 8
Acenaphthylene 81 9
Fluorene 99 9
Phenanthrene 98 9Fluoranthene 91 8
Chrysene 97 12
Benzo(b)-fluoranthene 60 8
Benzo(a)pyrene 55 8
Indene(1,2,3-c,d)pyrene 60 10
Benzo(g,h,i)perylene 59 10
Water Spiked at 50 ppb
Cl
Typical SPE Extraction Protocol for Opiates
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CI0126C 45
Sample Preparation:
• Add 0.5 mL hydrochloric acid to 5 mL urine
• Cap• Heat at 120°C for 20 min
• Cool to room temperature
• Add 0.75 mL 10 N NaOH
• Adjust to pH 6.5-7.5 with ~2.5 mL 0.5 M phosphoric acid
Condition:
• 6 mL methanol
• 6 mL 0.1 M K2HPO4 (pH 6.0)• Do not let the phase go dry
Load:
• Add 3 mL 0.1 M K2HPO4 to the cartridge
• Attach an 8 mL reservoir
• Add the urine sample
Rinse:
• Remove reservoir
• 3 mL water
• 3 mL 0.1 M sodium acetate (pH 4.5)
• 3 mL methanol
Elute:
• Place a collection tube beneath cartridge
• 3 mL methylene chloride/isopropyl alcohol/ NH4OH(78/20/2)
• Collect the eluant
Analysis Preparation:
• Concentrate the eluant to dryness
• Do not over dry• 75 µL ethyl acetate
• 25 µL BSTFA/1% TMCS
• Cap
• Heat at 60°C for 10 min
• Inject 1-2 µL
• All flow rates should not exceed 5 mL/min
Reagents:
• 10 N NaOH
40 g sodium hydroxide in 100 mL DI water.
• 0.1 M K2HPO4 (pH 6.0)1.74 g potassium phosphate, dibasic (anhydrous) in 100 mL DI
water. Adjust
to pH 6.0 ± 0.1 with phosphoric acid.
• 0.1 M sodium acetate (pH 4.5)0.82 g in 100 mL DI water. Adjust to
pH 4.5 ± 0.1 with glacial acetic acid.
• Methylene chloride/isopropyl
alcohol/NH4OH (78/20/2)
78 mL methylene chloride, 20 mL
isopropyl alcohol, 2 mL ammonium hydroxide. Make fresh daily.
• BSTFA/1% TMCS
9 mL N,O-bis(trimethylsilyl)trifluoroacetamide, 0.1 mLtrimethylchlorosilane, or can be purchased premixed.
Other reagents
Methanol
DI water
Ethyl acetate
Hydrochloric acid
Phosphoric acid
Certificate of Performance for AccuBONDII
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CI0126C 46
Description: AccuBOND C8 100mg 1mL cartridgeCatalog No: 188-0310
Lot No.: AMC18-0XRun No.: XXXXXXX
This AccuBOND product and sorbent have been manufactured, testedAnd assembled under the control of an ISO 9001 registered quality system.
This AccuBOND SPE product has been subjected to the following Q.C. tests:
Base Silica Characteristics Bonded Silica Tests
Surface Area: 546 m2/g Carbon Loading: 13.0 %Average Pore Size: 60 Å Surface Coverage: 2.5 µmoles/mSurface pH: 7 Extraction Residue: PassMetal Analysis: Pass Exchange Capacity: N/A
Particle Size Data
Particle Shape: Irregular Average Particle Size (µm): 56Percentage of Particles <10µm: 0.00Percentage of Particles <25µm: 1.36
Packed Cartridge Test
Cartridge Flow Resistance: PassCartridge Purity Test (GC): PassFrit Purity Test (GC): PassMaterial Weight Check: Pass
Percentage of Particles >90µm: 4.95
Test Condition and Results
Conditions:Column: 4.6 x 250 mm
Mobile Phase: 70/30 MeOH/H2O
Flow Rate: 2.0 ml/min
Specifications:
K’ biphenyl = 3.5 to 6.5
K’ toluene = 1.5 to 3.5
K’ acetophenone = 0.6 to 1.4
Tf uracil = 0.3 to 1.5
Results for Lot AMC8X-XX
K’ toluene = 4.5 Peak 1 = Uracil
K’ dimethylaniline = 2.1 Peak 2 = Acetophenone
K’ nitrobenzene = 1.0 Peak 3 = Toluene
Tf dimethylaniline = 1.4 Peak 4 = Biphenyl
Notice:
Material Safety Data Sheets (MSDS) are available at:
http://www.chem.agilent.com/scripts/cag_msdssearch.asp
This product has passed all Agilent Technologies, inc. quality control specifications.
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