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Mem Inst Oswaldo Cruz, Rio de Janeiro, Vol. 106(8): 957-967, December 2011
Search for a platelet-activating factor receptor in the Trypanosoma cruzi proteome: a potential target for Chagas disease chemotherapy
Daniel Fábio Kawano1/+, Vinicius Barreto da Silva1, Daniel Macedo de Melo Jorge2, Carlos Henrique Tomich de Paula da Silva1, Ivone Carvalho1
1Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto 2Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Av. do Café s/n, 14040-903 Ribeirão Preto, SP, Brasil
Chagas disease (CD) causes the highest burden of parasitic diseases in the Western Hemisphere and is therefore a priority for drug research and development. Platelet-activating factor (PAF) causes the CD parasite Trypanosoma cruzi to differentiate, which suggests that the parasite may express PAF receptors. Here, we explored the T. cruzi proteome for PAF receptor-like proteins. From a total of 23,000 protein sequences, we identified 29 hypothetical proteins that are predicted to have seven transmembrane domains (TMDs), which is the main characteristic of the G protein-coupled receptors (GPCRs), including the PAF receptor. The TMDs of these sequences were independently aligned with domains from 25 animal PAF receptors and the sequences were analysed for conserved residues. The conservation score mean values for the TMDs of the hypothetical proteins ranged from 31.7-44.1%, which suggests that if the putative T. cruzi PAF receptor is among the sequences identified, the TMDs are not highly conserved. These results suggest that T. cruzi contains several GPCR-like proteins and that one of these GPCRs may be a PAF receptor. Future studies may further validate the PAF receptor as a target for CD chemotherapy.
Key words: PAF - GPCR - Trypanosoma cruzi - Chagas disease - bioinformatics - proteome
processes, including inflammation, platelet aggrega-tion and immune response (Harayama et al. 2008). In T. cruzi, PAF proved to trigger cell differentiation to the infective and replicative parasite forms, suggesting the existence of specific molecular recognition sites that can be explored as potential drug targets (Rodrigues et al. 1996, Ambrósio et al. 2003). This hypothesis is sup-ported by the fact that PAF effects in T. cruzi are totally abrogated in the presence of the PAF receptor antago-nist WEB 2086, possibly acting via a seven transmem-brane domain (TMD) receptor, a G protein-coupled receptor (GPCR), as observed in mammals (Rodrigues et al. 1996). Additional studies also demonstrated that PAF was only active on intact parasite cells, suggesting the existence of PAF-mediated intracellular signalling mechanisms (Rodrigues et al. 1999).
Although endogenous PAF has been identified in T. cruzi and immunofluorescence assays indicate that PAF binding sites are present on the parasite membrane, at-tempts to isolate T. cruzi PAF receptors have failed (Gomes et al. 2006). We hypothesised that these PAF receptors proteins are encoded by the T. cruzi genome, which has already been sequenced (Atwood 3rd et al. 2005).
Recent advances in bioinformatics have facilitated the discovery of new drug targets (Chen & Chen 2008, Alves-Ferreira et al. 2009). Here, we evaluated bioinformatic approaches that were previously used to discover new GPCRs in fungi, plants and invertebrates (Kulkarni et al. 2005, Gookin et al. 2008, Kamesh et al. 2008) to identify PAF receptor-like proteins in the T. cruzi proteome.
MATERIALS AND METHODS
The PAF receptor sequences from 25 different ani-mal species were retrieved from Uniprot (uniprot.org/) and ENSEMBL (ensembl.org/) (Supplementary data).
Financial support: FAPESP+ Corresponding author: [email protected] 8 July 2011Accepted 8 November 2011
Chagas disease (CD), or American trypanosomiasis, is a tropical parasitic disease that affects approximately 10 million people in Latin America (WHO 2010). CD is caused by the flagellated protozoan Trypanosoma cruzi, which is often transmitted to humans and other mammals by blood-sucking insects, such as the tri-atomines (Urbina et al. 2003). Transmission can also occur via blood transfusion (Young et al. 2007), organ transplantation (Kun et al. 2009), ingestion of contami-nated foods or fluids (Benchimol Barbosa 2006) or congenital acquisition (Dorn et al. 2007). CD kills ap-proximately 10,000 people every year (WHO 2010) and has a direct impact on workforce productivity in Latin America (Conteh et al. 2010).
Although CD causes the highest burden of parasitic diseases in the Western Hemisphere (Bern & Montgomery 2009), current treatments are highly toxic and often inef-fective, particularly for the chronic stage of the disease (Trouiller et al. 2002). Strikingly, no drug has emerged as an effective candidate for clinical trials in the last 30 years (Moreira et al. 2009). Therefore, the identification of an enzyme or receptor that is essential for parasite growth and/or survival in vivo may facilitate the devel-opment of safer and more effective drugs (Wang 1997).
Platelet-activating factor (PAF) is a potent lipid me-diator that plays a role in several human physiological
Search for PAF receptor in T. cruzi • Daniel Fábio Kawano et al.958
The T. cruzi proteome was downloaded from TriTrypDB (tritrypdb.org/) (Aslett et al. 2010).
Phylogenetic analyses - The PAF receptor sequenc-es were converted to Molecular Evolutionary Genetics Analysis (MEGA) file format with the MEGA 4.0 soft-ware (Tamura et al. 2007) and then aligned using Multi-ple Sequence Alignment by Log-Expectation (MUSCLE) (Edgar 2004). The phylogenetic analyses were performed with MEGA with the neighbour-joining algorithm (Saitou & Nei 1987). The bootstrap support values for the op-timal trees were calculated using 1,000 replicates with heuristic search settings that were identical to the origi-nal search settings (Felsenstein 1985). The evolutionary distances were calculated with the Jones-Taylor-Thornton matrix-based method (Jones et al. 1992) with respect to the units of the number of amino acid (AA) mutations per site. The indels were treated as missing data.
PAF receptor conserved residue analysis - The resi-dues that are totally conserved among PAF receptors were analysed with MEGA through multiple sequence alignments. The sequences that correspond to the seven membrane-spanning TMDs of the PAF receptors were inferred by alignment to the published human PAF re-ceptor sequence (Gui et al. 2007).
ConSurf-conservation analysis (Ashkenazy et al. 2010) was performed using the tertiary structure of the human PAF receptor as the three-dimensional scaffold (PDB code 2B0X). Multiple sequence alignments of the 25 PAF receptors were computed using MUSCLE (Edgar 2004), and the Bayesian method (Mayrose et al. 2004) was used to calculate the conservation scores with the Jones-Taylor-Thornton matrix-based method (Jones et al. 1992) as a model for protein substitutions.
Search for PAF receptor homologues - The search for PAF receptor homologues was performed with the Basic Local Alignment Search Tool (BLAST)P algo-rithm (Altschul et al. 1990) in the National Center for Biotechnology Information (NCBI) BLAST package version 2.2.24. The search was optimised to detect or-thologues by selecting soft filtering (chosen in the stand-alone NCBI version of BLAST with the -F ‘‘m S’’ op-tion) and the Smith-Waterman algorithm was used to produce the final alignments and calculate the scores (-s T option) (Moreno-Hagelsieb & Latimer 2008). The T. cruzi proteome was used as query in BLASTP against PAF receptor sequences with an e-value threshold of 10 (default). To decrease the possibility of missing a puta-tive PAF receptor sequence, we selected all sequences that were above the threshold value for conservational analyses, not just the reciprocal best hits, which are typi-cally considered for orthologue detection.
T. cruzi hypothetical protein conservation analysis - The T. cruzi sequences that were obtained from the BLAST searches were classified by protein description and the ConPred II software was used to select proteins that have a predicted transmembrane topology. The pro-gram predicts TMDs based on a consensus approach by combining the results of several methods, including KKD, TMpred, TopPred II, DAS, TMAP, MEMSAT 1.8, SOSUI, TMHMM 2.0 and HMMTOP 2.0 (Arai et al.
2004). The theoretical molecular weights of these pro-teins were predicted using the Sequence Manipulation Suite tool (bioinformatics.org/sms2/protein_mw.html).
The sequences of hypothetical proteins that were predicted to contain seven TMDs were manually edited to separate the transmembrane regions, which were then independently aligned with the corresponding TMDs of the 25 PAF receptors using MUSCLE (Edgar 2004). A conservation score for each AA position in the TMD multiple sequence alignment was computed with the SCORECONS web server with default parameters (ebi.ac.uk/thornton-srv/databases/cgi-bin/valdar/scorecons_server.pl) (Valdar 2002). The method generates conser-vation scores for individual residues based on norma-lised substitution matrices or multiple alignments of a set of sequences (Valdar 2002). To reduce the apparent weight assigned to the PAF receptor sequences in the fi-nal score, we highlighted conservation in each AA posi-tion of the hypothetical proteins and rescaled the scores between 0-99, as previously proposed by Haubertin et al. (2006): Rscore = 99 - [99 × (S - Lscore)/Δscore], where Rscore is the rescaled score, S is the score calculated with SCORECONS, Lscore is the lowest score for a giv-en AA position and Δscore is the amplitude of all of the scores for the AA residue. The Rscores that were derived from gapped positions in the T. cruzi hypothetical pro-tein sequences with respect to the scaffold were defined as zero. With the rescaled scores from each AA residue, the weighted means were calculated for each TMD us-ing the following formula, x = ∑(x.w)/∑w , where x is the weighted mean, x the Rscore value for a given AA residue and w is the corresponding conservation weight (score) calculated with SCORECONS when only the multiple sequence alignment of the 25 PAF receptor sequences was used as the input. The final conserva-tion scores were calculated by the weighted mean of the rescaled scores regarding all AA residues in the seven TMDs predicted for the given hypothetical protein.
RESULTS
We assessed the evolutionary relationship of PAF re-ceptors from 25 different animal species (Supplementa-ry data). To the best of our knowledge, these are the only PAF receptor sequences that are currently available. The phylogenetic tree (Fig. 1) clearly indicates that these PAF receptors are split into two main branches. One cluster contains the mammals and the amphibian/bird clades and the second cluster exclusively contains fish. The mammalian lineage can be further subdivided into two branches, where the opossum and platypus are separated from the mammals. The human, orangutan and chim-panzee PAF receptors were sub-grouped together.
The multiple sequence alignment of the PAF recep-tors (Supplementary data) shows that 77 AAs are com-pletely conserved among the 25 species. The majority of these residues (approximately 82%) are located in the TMDs (Fig. 2A, Table I). When residues that are par-tially conserved among species are analysed (shown in pink in Fig. 2B), the alignment clearly shows that these residues are also in the TMDs, except for the fourth do-main, which is the most variable TMD (Table I).
959Mem Inst Oswaldo Cruz, Rio de Janeiro, Vol. 106(8), December 2011
To identify T. cruzi PAF receptor protein sequences, the 23,031 total T. cruzi protein sequences available in TriTrypDB (Aslett et al. 2010) were compared to other the 25 PAF receptor sequences via a BLAST search with an E-value threshold of 10. A total of 264 parasite pro-tein sequences were selected, of which 138 were identi-fied as potential PAF receptor proteins. A comparison of these hypothetical proteins with the PAF receptors yields similar BLAST best score values that range from 17-29. The hypothetical protein Tc00.1047053511507.60 presented the highest number of BLAST hits: 24 from the 25 PAF receptor sequences (Table II).
Of the 138 hypothetical T. cruzi proteins identified, 29 sequences were predicted to contain seven TMDs with chain lengths that ranged from 305-1,410 AA. TMD se-quence of these hypothetical proteins was analysed for AA conservation and the resulting mean values for the seven TMDs ranged from 31.7-44.1%. When the TMDs were analysed independently, no T. cruzi hypothetical protein exhibited a weighted mean value above 60% (Table III).
DISCUSSION
Several groups have previously proposed that PAF exerts a biological effect on T. cruzi by binding to trans-membrane receptors (Rodrigues et al. 1996, Ambrósio et
al. 2003, Gomes et al. 2006). PAF receptors are GPCRs, which are transmembrane signalling proteins that are evolutionarily conserved among eukaryotic organisms (Römpler et al. 2007). The occurrence of GPCRs and G-protein signalling dates back 1.2 billion years, which was
Fig. 1: evolutionary relationships of platelet-activating factor (PAF) re-ceptors from 25 animal species. The evolutionary relationship of PAF receptors was inferred using the neighbour-joining method (Saitou & Nei 1987). The bootstrap consensus tree inferred from 1,000 repli-cates (Felsenstein 1985) is taken to represent the evolutionary history of the taxa analyzed. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to in-fer the phylogenetic tree. The evolutionary distances were computed using the Jones-Taylor-Thornton matrix-based method (Jones et al. 1992) and are in the units of the number of amino acid (AA) substitu-tions per site. The analysis involved 25 AA sequences. All positions containing gaps and missing data were eliminated. There were a to-tal of 281 positions in the final dataset. Evolutionary analyses were conducted in Molecular Evolutionary Genetics Analysis 4.0 software (Tamura et al. 2007).
Fig. 2A: conservation pattern of platelet-activating factor (PAF) re-ceptors from 25 animal species. Residues totally conserved among re-ceptors are highlighted in yellow. Multiple sequence alignments were performed using Multiple Sequence Alignment by Log-Expectation (MUSCLE) (Edgar 2004) and the analysis of conserved residues was carried out in Molecular Evolutionary Genetics Analysis (MEGA) 4.0 software (Tamura et al. 2007). The two-dimensional representa-tion of human PAF receptor was adapted from Gui et al. (2007); B: conservation profile for the tertiary structure of PAF receptor (PDB code 2B0X) using ConSurf-conservational analysis. Protein was vi-sualized using FirstGlance in Jmol with colour-coded conservation scores. The conserved and variable residues are coloured according to the conservation scores. Sites, for which the inferred conservation level was assigned with low confidence, are coloured light yellow. Multiple sequence alignments of the 25 PAF receptors were computed using MUSCLE and the Bayesian method (Mayrose et al. 2004) was applied for the calculations of the conservation scores using the Jones-Taylor-Thornton matrix-based method (Jones et al. 1992) as the model of substitution for proteins.
Search for PAF receptor in T. cruzi • Daniel Fábio Kawano et al.960
before plants, fungi and animals emerged from a common ancestor (Schöneberg et al. 2007). Thus, it seems reason-able that T. cruzi may also express a PAF receptor.
Current theory regarding receptor structure and function evolution suggests that the structural diversity of the receptor between different species is a result of evolution, which is characterised by the continuous ac-cumulation of mutations. However, if receptor function is essential for survival of the organism, some struc-tural conservation is required to ensure protein function (Schöneberg et al. 2007).
Our studies regarding PAF receptor evolution indi-cate that the TMDs have the highest density of conserved residues. This observation is consistent with molecular modelling studies, which suggest that the ligand-binding site of the human PAF receptor is fundamentally com-posed of TMD residues (Gui et al. 2007). Moreover, the TMDs tend to be conserved among GPCRs, while the extra and intracellular loop regions and C-terminus are typically more divergent (Herz & Thomsen 2007).
Based on similarity to known PAF receptor protein sequences, we identified 29 hypothetical seven TMD proteins in the T. cruzi proteome. To the best of our knowledge, these proteins represent the first putative GPCRs identified in this parasite. Although the evo-lutionary origins of the encoding genes remain un-known, previous studies have indicated that horizontal gene transfer (the process where genetic material is transferred between distinct evolutionary lineages) has occurred in parasitic protozoa (Richards et al. 2003); moreover, viral infection may explain the origin of sev-eral mammalian genes, including genes that code for membrane receptors (Yu et al. 1996).
An alternative hypothesis is that putative GPCRs have been present since the beginning of trypanosome
evolution. For example, the discovery of specific GP-CRs in the protozoan Dictyostelium discoideum that were previously thought to be only in animals suggests that these genes arose before the divergence of animals and fungi and were subsequently lost in fungi (Prabhu & Eichinger 2006).
Our evolutionary analysis of previously identified PAF receptors indicates that the fish receptor sequences are grouped into a distinct clade from the other PAF re-ceptors. Danio rerio, Gasterosteus aculeatus, Oryzias latipes and Tetraodon nigroviridis are teleost fishes, some of the most primitive vertebrates (Verburg-van Ke-menade et al. 2009), thus pointing to the use of a PAF re-ceptor sequence from a fish (instead of from a human) as the reference to calculate the percentages of un-gapped AA residues in the conservation analyses of hypotheti-cal proteins. However, in view of the considerable evolu-tionary distance between GPCRs from animals and their putative counterparts in T. cruzi (Wilkie & Kinch 2005), we believe that the advantage of using a PAF receptor sequence from a fish as the reference could be assumed as insignificant. Besides, the fact that both PAF and its antagonist WEB 2086 are active in human and T. cruzi (Rodrigues et al. 1996) suggests some conservation be-tween these species, but the same cannot be stated for fishes and T. cruzi.
A comparison of the identified T. cruzi seven TMD proteins and the human PAF receptor sequence resulted in relatively similar low BLAST scores. However, the BLAST algorithm typically does not provide high scores among phylogenetically distant species because genom-es with a large evolutionary distance typically have a low protein sequence similarity (Kim et al. 2008). Thus, it would be optimal to use PAF receptor sequences from more primitive organisms, such as Dictyostelium or En-tamoeba, which have genes that encode GPCRs (Wilkie & Kinch 2005). However, because no PAF receptor se-quence from a lower eukaryote is currently available, a more precise relationship cannot be established.
Conservation analyses of the TMD AAs of the hy-pothetical T. cruzi proteins did not allow us to identify a protein sequence that is orthologous to the human PAF receptor. Although the weighted mean conserva-tion scores that we calculated cannot be assumed to be absolute percentages of AA conservation relative to the PAF receptors, we expect that a putative PAF receptor orthologue would exhibit a higher number of conserved motifs; consequently, this should be expressed as a con-siderably higher conservation score compared to the re-maining non-orthologous proteins. Nevertheless, if the putative T. cruzi PAF receptor is among the sequences we identified, this receptor does not show a high degree of conservation in the TMDs compared to animal PAF receptors. This is apparent in the protozoan D. discoi-deum, where the seven TMD receptors show little se-quence similarity to the corresponding GPCRs in ani-mals (Lagerström & Schiöth 2008).
The 29 putative seven TMD proteins identified in this study represent a starting point for additional bio-chemical, physiological and molecular biology studies
TABLE IConserved residues in the transmembrane domains of
platelet-activating factor (PAF) receptors from 25 animal species analyses of residues that are totally conserved among
PAF receptors were carried out in Molecular Evolutionary Genetics Analysis (Tamura et al. 2007) and residues partially
conserved were computed using ConSurf-conservation analysis (Ashkenazy et al. 2010)
Domain
Residues totallyconserved
(n)
Residues partiallyconserve
(n)
Total of residues
(n)
I 10 18 30II 12 23 30III 13 21 33IV 4 7 23V 3 11 26VI 10 24 31VII 11 24 31
961Mem Inst Oswaldo Cruz, Rio de Janeiro, Vol. 106(8), December 2011
TABLE IIHypothetical proteins of Trypanosoma cruzi detected via Basic Local Alignment Search Tool (BLAST)
search against platelet-activating factor (PAF) receptors from 25 animal species prediction of transmembrane domains (TMDs) was carried out with ConPred II software (Arai et al. 2004)
Hypothetical protein
BLAST
Length(AA)
Weight(kDa) TMDsHits
Bestscore e-value PAF
Tc00.1047053511573.49 3 29 0.002 Rat 235 27.26 6Tc00.1047053509027.50 19 27 0.041 Rat 534 59.23 6Tc00.1047053503487.70 17 26 0.031 Elephant 382 41.66 8Tc00.1047053511127.300 2 26 0.071 Squirrel 534 59.42 6Tc00.1047053510493.10 5 25 0.057 Mouse 240 25.82 6Tc00.1047053508801.10 12 25 0.10 Opossum 372 41.71 8Tc00.1047053508641.200 5 24 0.14 Elephant 305 32.55 7Tc00.1047053506869.20 3 24 0.20 Platypus 458 52.67 8Tc00.1047053510519.100 3 24 0.20 Platypus 458 52.64 8Tc00.1047053508815.80 3 24 0.23 Chicken 570 64.11 9Tc00.1047053510311.20 13 24 0.30 Frog 801 91.08 6Tc00.1047053508873.530 10 24 0.35 Platypus 1,071 115.86 6Tc00.1047053511629.40 4 23 0.12 Mouse 240 25.84 6Tc00.1047053511285.80 16 23 0.18 Pika 247 27.23 6Tc00.1047053509649.40 16 23 0.19 Pika 247 27.25 6Tc00.1047053508547.140 1 23 0.26 Zebrafish 482 53.43 8Tc00.1047053507943.70 3 23 0.27 Medaka 405 43.59 9Tc00.1047053506681.24 8 23 0.35 Horse 341 38.37 8Tc00.1047053508215.6 4 23 0.40 Platypus 494 55.71 7Tc00.1047053506925.450 3 23 0.40 Opossum 681 77.39 6Tc00.1047053503463.20 4 23 0.41 Platypus 494 55.69 7Tc00.1047053510725.70 4 23 0.46 Opossum 486 54.09 6Tc00.1047053509099.130 16 23 0.50 Human 822 91.69 8Tc00.1047053510885.60 19 23 0.72 Guinea pig 1,151 131.20 6Tc00.1047053508159.26 3 23 1.1 Guinea pig 1,278 137.16 4Tc00.1047053511183.315 2 23 1.1 Medaka 1,085 115.00 4Tc00.1047053510305.79 10 23 1.2 Medaka 1,410 156.58 7Tc00.1047053507941.110 3 22 0.44 Medaka 385 41.43 9Tc00.1047053506825.24 7 22 0.48 Horse 341 38.44 7Tc00.1047053510729.290 20 22 0.62 Platypus 335 37.98 7Tc00.1047053506467.4 3 22 0.67 Stickleback 340 38.27 7Tc00.1047053504137.150 8 22 0.68 Stickleback 340 38.52 7Tc00.1047053505193.74 4 22 0.69 Stickleback 340 38.21 7Tc00.1047053509075.31 3 22 0.70 Mouse 540 58.70 5Tc00.1047053509561.70 11 22 0.72 Pika 306 34.20 6Tc00.1047053510103.30 17 22 0.81 Opossum 594 65.97 5Tc00.1047053506009.60 4 22 0.82 Guinea pig 416 45.27 6Tc00.1047053506179.30 1 22 0.97 Chicken 600 66.99 8Tc00.1047053507663.80 1 22 0.99 Chicken 600 66.77 8
Search for PAF receptor in T. cruzi • Daniel Fábio Kawano et al.962
Hypothetical protein
BLAST
Length(AA)
Weight(kDa) TMDsHits
Bestscore e-value PAF
Tc00.1047053509719.56 2 22 1.2 Zebrafish 724 82.56 7Tc00.1047053511461.40 5 22 1.3 Pig 552 62.88 6Tc00.1047053508535.30 4 22 1.3 Pig 553 63.02 7Tc00.1047053508443.50 3 22 1.5 Pufferfish 630 70.30 5Tc00.1047053509157.110 3 22 1.5 Pufferfish 630 70.46 4Tc00.1047053507821.101 2 22 1.5 Rat 994 106.93 4Tc00.1047053507209.60 16 22 1.6 Guinea pig 819 91.35 8Tc00.1047053508153.920 3 22 1.6 Platypus 611 68.86 1Tc00.1047053506529.150 1 22 1.8 Zebrafish 1,151 131.49 6Tc00.1047053511921.71 9 22 1.9 Opossum 1,157 124.18 7Tc00.1047053509849.30 4 22 2.2 Shrew 860 97.79 7Tc00.1047053511185.150 1 22 3.2 Guinea pig 2,247 243.13 -Tc00.1047053508891.80 1 22 4.5 Mouse 1,935 216.30 6Tc00.1047053507615.60 2 21 0.76 Frog 284 32.16 6Tc00.1047053508277.210 21 21 0.81 Stickleback 319 36.83 6Tc00.1047053511495.20 4 21 0.81 Elephant 324 36.45 6Tc00.1047053508489.20 4 21 0.82 Elephant 324 36.39 6Tc00.1047053508799.290 4 21 0.83 Pufferfish 221 24.45 6Tc00.1047053506753.30 19 21 0.87 Elephant 341 37.88 7Tc00.1047053511305.40 4 21 0.88 Chicken 301 32.92 5Tc00.1047053509945.24 22 21 0.89 Stickleback 319 36.79 6Tc00.1047053507257.180 2 21 0.94 Medaka 327 35.92 5Tc00.1047053510355.190 20 21 0.97 Elephant 341 37.92 7Tc00.1047053511507.60 24 21 1.1 Shrew 311 35.11 7Tc00.1047053507883.50 20 21 1.1 Pika 319 35.46 5Tc00.1047053503897.120 11 21 1.1 Pika 306 34.20 5Tc00.1047053510001.31 4 21 1.1 Medaka 317 34.99 6Tc00.1047053503647.40 1 21 1.1 Chicken 409 45.33 9Tc00.1047053508741.20 2 21 1.2 Medaka 319 34.70 9Tc00.1047053506195.50 15 21 1.3 Pika 319 35.29 5Tc00.1047053508041.20 12 21 1.4 Goat 528 60.25 6Tc00.1047053509997.73 3 21 1.4 Platypus 351 38.97 8Tc00.1047053506297.340 12 21 1.5 Goat 528 60.22 6Tc00.1047053507603.220 3 21 1.5 Chicken 444 49.64 6Tc00.1047053506661.140 3 21 1.5 Pika 486 54.79 6Tc00.1047053509269.19 7 21 1.7 Pufferfish 471 53.80 8Tc00.1047053509429.280 5 21 1.9 Chicken 552 62.15 8Tc00.1047053504021.90 1 21 2.3 Guinea pig 600 65.97 9Tc00.1047053507083.100 3 21 2.5 Chicken 662 72.90 8Tc00.1047053507677.60 1 21 2.5 Guinea pig 600 65.87 9Tc00.1047053508317.70 4 21 2.6 Shrew 856 97.38 7Tc00.1047053503735.30 1 21 2.6 Horse 844 95.54 7Tc00.1047053509901.100 3 21 2.9 Goat 662 72.94 8
963Mem Inst Oswaldo Cruz, Rio de Janeiro, Vol. 106(8), December 2011
Hypothetical protein
BLAST
Length(AA)
Weight(kDa) TMDsHits
Bestscore e-value PAF
Tc00.1047053508121.15 2 21 3.1 Chicken 1,027 111.03 4Tc00.1047053510507.60 2 21 4.0 Cow 1,130 127.09 5Tc00.1047053503919.60 1 21 6.1 Squirrel 1,579 177.58 5Tc00.1047053507723.70 1 21 9.1 Rabbit 2,960 326.30 -Tc00.1047053509799.90 4 20 1.4 Frog 310 35.33 7Tc00.1047053509431.10 5 20 1.5 Cow 297 33.22 5Tc00.1047053508739.30 5 20 1.6 Pika 331 36.04 8Tc00.1047053503909.90 23 20 1.7 Mouse 311 35.14 6Tc00.1047053508241.104 2 20 1.7 Stickleback 295 33.58 6Tc00.1047053511693.130 6 20 1.8 Squirrel 383 43.59 8Tc00.1047053506565.29 2 20 2.4 Chicken 425 49.18 9Tc00.1047053509245.29 1 20 2.4 Opossum 315 35.83 8Tc00.1047053507969.30 6 20 2.5 Opossum 470 53.77 7Tc00.1047053504029.90 4 20 2.7 Guinea pig 351 38.96 8Tc00.1047053509799.100 7 20 2.8 Stickleback 340 38.66 7Tc00.1047053511245.24 3 20 2.8 Pika 486 54.56 6Tc00.1047053510311.80 4 20 3.3 Platypus 655 72.93 8Tc00.1047053511801.4 1 20 3.7 Goat 634 70.95 6Tc00.1047053511001.50 3 20 4.3 Shrew 494 54.09 7Tc00.1047053503593.60 1 20 4.4 Medaka 654 72.40 5Tc00.1047053506127.120 1 20 4.5 Medaka 654 72.17 5Tc00.1047053511279.20 2 20 4.7 Pika 709 79.47 6Tc00.1047053508723.24 1 20 4.9 Frog 559 63.22 7Tc00.1047053509791.60 1 20 4.9 Frog 559 63.25 7Tc00.1047053511923.71 10 20 5.0 Mouse 1,103 120.80 7Tc00.1047053509897.132 9 20 5.4 Pika 1,129 119.93 5Tc00.1047053508741.260 1 20 5.5 Elephant 814 90.46 8Tc00.1047053511003.160 2 20 6.2 Rat 1,034 114.04 6Tc00.1047053504033.140 1 20 6.4 Chicken 1,013 113.12 6Tc00.1047053511787.30 1 20 6.9 Rat 1,267 134.46 7Tc00.1047053510861.94 1 20 8.0 Horse 845 95.95 7Tc00.1047053509899.70 1 20 9.0 Pufferfish 1,271 135.75 6Tc00.1047053508347.140 1 19 2.8 Frog 284 32.08 6Tc00.1047053504765.20 17 19 2.9 Pig 269 30.04 6Tc00.1047053504137.140 4 19 3.6 Frog 310 35.44 7Tc00.1047053505757.10 10 19 3.7 Rat 259 28.18 5Tc00.1047053506669.10 10 19 3.7 Rat 259 28.18 5Tc00.1047053510885.20 3 19 3.9 Horse 296 32.15 6Tc00.1047053506529.110 2 19 3.9 Horse 296 32.08 6Tc00.1047053511837.40 1 19 4.1 Medaka 290 32.68 8Tc00.1047053511531.44 2 19 4.7 Platypus 405 44.45 5Tc00.1047053509741.20 1 19 5.2 Stickleback 356 39.50 4Tc00.1047053507681.50 1 19 5.3 Platypus 516 57.64 7
Search for PAF receptor in T. cruzi • Daniel Fábio Kawano et al.964
Hypothetical protein
BLAST
Length(AA)
Weight(kDa) TMDsHits
Bestscore e-value PAF
Tc00.1047053507509.80 2 19 5.7 Cow 573 63.50 6Tc00.1047053506871.130 2 19 5.9 Cow 573 63.64 6Tc00.1047053507395.10 8 19 6.4 Elephant 405 45.36 6Tc00.1047053510087.30 7 19 6.4 Elephant 403 45.13 5Tc00.1047053510265.14 1 19 7.5 Rabbit 655 72.97 8Tc00.1047053511471.10 1 19 8.7 Rabbit 796 86.81 1Tc00.1047053430895.10 11 18 4.0 Rat 259 28.24 5Tc00.1047053509253.30 1 18 5.1 Stickleback 265 30.50 5Tc00.1047053508207.120 2 18 6.0 Rat 224 25.28 5Tc00.1047053511683.20 2 18 6.1 Medaka 318 34.60 9Tc00.1047053507625.200 1 18 6.1 Stickleback 355 39.56 9Tc00.1047053402857.10 1 18 7.9 Stickleback 321 34.93 8Tc00.1047053506513.14 1 17 9.0 Guinea pig 222 25.39 6
AA: amino acid.
TABLE IIIConservation analysis of amino acid (AA) residues in the transmembrane domains (TMDs)
of Trypanosoma cruzi hypothetical proteins performed in SCORECONS web server (Valdar 2002) conservation scores for each TMD are expressed as the weighted mean of rescaled scores for each AA position. Percentages of un-gapped
AA residues in the seven TMDs were calculated in comparison to the sequence of human PAF receptor in the multiple sequence alignment containing each hypothetical protein and the sequence of 25 PAF receptors.
Hypothetical proteinLength (AA)
Un-gapped TMDs
residues (%)
Conservation scores (%)
Mean(7 TMDs)
Domains
I II III IV V VI VII
Tc00.1047053508215.6 494 89.1 44.1 55.5 49.3 43.5 44.5 35.3 33.1 45.8Tc00.1047053508723.24 559 83.7 42.9 39.1 38.3 52.4 50.7 44.3 38 38.7
Tc00.1047053509791.60 559 83.7 42.8 39.1 38.2 52.4 50.7 44.3 38 38.7
Tc00.1047053506753.30 341 87.8 42.8 51.1 36.4 41.9 46,3 42.7 28.4 52.5
Tc00.1047053505193.74 340 91.2 42.5 52.1 38.3 45.5 31.3 34.9 44.9 48.2
Tc00.1047053503463.20 494 87.1 42.5 44.7 48.6 43.5 44.5 36.3 33.1 45.8
Tc00.1047053504137.150 340 89.8 42.2 44.3 30.1 42.4 51.3 52.5 47.8 31
Tc00.1047053506467.4 340 91.2 42.2 52.1 38.3 45.5 30.2 34.9 44.9 46.9
Tc00.1047053509799.100 340 89.8 42.1 44.3 30.1 42.4 51.3 52.5 46.7 31
Tc00.1047053508641.200 305 87.8 41.7 48.3 37.1 40.6 41.6 46.5 50.3 28.9
Tc00.1047053511787.30 1,267 89.1 41 29 38.6 36.6 37 51.1 43.7 51.2
Tc00.1047053510305.79 1,410 88.4 40.9 44.1 34.2 31.8 41.6 40 45.5 49.1
Tc00.1047053510861.94 845 95.2 40.8 39.4 35.9 34.7 47.5 42.4 41.4 45.5
Tc00.1047053511923.71 1,103 81.6 40.6 39.9 39.5 40.1 40.8 44.4 25.4 53.3
Tc00.1047053511921.71 1,157 89.8 40.6 39.9 44.9 38.6 30.4 50.5 24.4 53.3Tc00.1047053503735.30 844 92.5 40.6 39.4 38.4 33 47.5 42.4 41.4 43.4
965Mem Inst Oswaldo Cruz, Rio de Janeiro, Vol. 106(8), December 2011
to identify surface proteins in T. cruzi. None of these 29 sequences or any other sequence derived from hy-pothetical proteins in the parasite proteome display a high degree of similarity with the human PAF receptor. However, the identification of the T. cruzi receptor may theoretically enable the design of PAF antagonists that interact with the T. cruzi, but not human PAF receptor. Molecules that affect the growth/differentiation of para-sites can subsequently be tested as selective and effec-tive drugs for the treatment of neglected diseases (Ham-marton et al. 2003).
The limitations of this study include the ability of the BLAST algorithm to correctly identify the putative PAF receptor sequence and the ability to correctly pre-dict TMD structures. To minimise these limitations, we selected a BLASTP algorithm that has been optimised to detect orthologues (Moreno-Hagelsieb & Latimer 2008) and used a consensus approach to properly detect the TMDs (Arai et al. 2004).
Additional limitations of this work may be related to the nature of the proteomic data analysed. In contrast to the kinetoplastid pathogens Leishmania major and Try-panosoma brucei, the T. cruzi assembled genome se-quences are considered to be highly repetitive because the CL Brener reference strain is a hybrid of the “non-Esmeraldo-like” and “Esmeraldo-like” lineages; conse-quently, each chromosome is comprised of two homolo-gous chromosomes (Alves-Ferreira et al. 2009, Weatherly et al. 2009). Therefore, the predicted T. cruzi proteome has some inherent restrictions regarding computational gene prediction and annotation that may hamper the iden-tification of a potential unpredicted and/or un-annotated PAF receptor. Additional studies regarding the identifi-
Hypothetical proteinLength (AA)
Un-gapped TMDs
residues (%)
Conservation scores (%)
Mean(7 TMDs)
Domains
I II III IV V VI VII
Tc00.1047053510355.190 341 87.8 40.5 51.1 36.4 25.9 46.3 41.1 32.2 50.8Tc00.1047053509719.56 724 86.4 40.2 34.7 36.7 46 43.5 42.2 30.9 47.2
Tc00.1047053508317.70 856 86.4 40.1 31.4 38.6 57.3 43 40.4 40.2 30.3
Tc00.1047053509849.30 860 86.4 38.6 47.1 31 38.4 44.6 40.4 40.2 30.3
Tc00.1047053510729.290 335 90.5 38.5 48.7 31.7 43.2 41.7 33.8 36.8 33.7
Tc00.1047053504137.140 310 90.5 38.2 41.9 28.3 44.1 31 36.5 32.8 50.7
Tc00.1047053509799.90 310 93.2 37.7 47.9 28.3 44.1 32.5 33 32.8 44.1
Tc00.1047053507969.30 470 93.2 37.7 36.5 27.2 59.4 39.6 31.8 34.3 35.3
Tc00.1047053511001.50 494 86.4 37.7 33.2 34.6 26.4 41.2 34.9 43.4 50.3
Tc00.1047053507681.50 516 89.8 36.7 48.1 26.2 34.3 34.9 32 41.8 39.4
Tc00.1047053506825.24 341 87.1 34.7 40.1 28.1 25.9 38 42.6 31.3 38
Tc00.1047053511507.60 311 85.7 34.5 31.7 27.4 33.4 36.4 43.8 35.1 35.2Tc00.1047053508535.30 553 87.1 31.7 39.5 19.5 10.7 42.4 43.4 31.7 38
cation of PAF receptor homologues in the complete T. cruzi genome (not only against the predicted proteome) are needed, as they may overcome these limitations.
ACKNOWLEDGEMENTS
To Prof. Marcelo Dias Baruffi, for the continuous support throughout the course of this project.
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1Supplementary data Search for PAF receptor in T. cruzi • Daniel Fábio Kawano et al.
Sequences platelet-activating factor (PAF) receptors
PAF Sequence ID Source
Bos taurus (cow) Q9TTY5 UniprotCapra hircus (goat) Q9GK76 UniprotCavia porcellus (guinea pig) P21556 UniprotGallus gallus (chicken) E1C095 UniprotHomo sapiens (human) P25105 UniprotMacaca mulatta (rhesus monkey) P35366 UniprotMus musculus (mouse) Q62035 UniprotRattus norvegicus (rat) P46002 UniprotSus scrofa (pig) Q9XSD4 UniprotXenopus laevis (African clawed frog) Q7ZWY0 UniprotDanio rerio (zebrafish) ENSDARP00000094880 Ensembl Equus caballus (horse) ENSECAP00000003792 Ensembl Erinaceus europaeus (hedgehog) ENSEEUP00000012269 Ensembl Gasterosteus aculeatus (three-spined stickleback) ENSGACP00000012761 Ensembl Loxodonta africana (elephant) ENSLAFP00000006539 Ensembl Monodelphis domestica (opossum) ENSMODP00000032054 Ensembl Ochotona princeps (American pika) ENSOPRP00000002162 Ensembl Ornithorhynchus anatinus (platypus) ENSOANP00000021295 Ensembl Oryctolagus cuniculus (rabbit) ENSOCUP00000015312 Ensembl Oryzias latipes (medaka) ENSORLP00000015743 Ensembl Pan troglodytes (chimpanzee) ENSPTRP00000000744 Ensembl Pongo pygmaeus (orangutan) ENSPPYP00000001903 Ensembl Sorex araneus (shrew) ENSSARP00000005182 Ensembl Spermophilus tridecemlineatus (squirrel) ENSSTOP00000014258 Ensembl Tetraodon nigroviridis (green pufferfish) ENSTNIP00000020395 Ensembl
2Supplementary data Search for PAF receptor in T. cruzi • Daniel Fábio Kawano et al.
ConSsurf colour-coded multiple sequence alignment of platelet-activating factor receptors
3Supplementary data Search for PAF receptor in T. cruzi • Daniel Fábio Kawano et al.
Mul
tiple
sequ
ence
alig
nmen
ts o
f tra
nsm
embr
ane
dom
ains
from
Try
pano
som
a cr
uzi h
ypot
hetic
al p
rote
ins a
nd p
late
let-a
ctiv
atin
g fa
ctor
rece
ptor
sSe
quen
ceD
omai
n I
Dom
ain
IID
omai
n II
ID
omai
n IV
Dom
ain
VD
omai
n V
ID
omai
n V
IITc
00.1
0470
5350
8215
.6--S
RVW
FVAF
TFGM
TGHS
SVM
TM-
-PIM
ISCT
LLPL
VFLG
FLLR
I---
----C
LGFL
SDAF
VLM
FGIT
ALAS
Q---
FLGF
SILI
SCTR
LVSI
LILF
PVA
VFPI
MFG
YAFC
GHVV
FGAF
------
--SRS
IVTL
FCTT
FGDN
IIDTF
L-F
GRLF
IGTY
LLL-
-FIC
NIL
NVA
Hum
an-F
PIVY
SIIF
VLGV
IAN
GYVL
W--
--FM
VNLT
MAD
MLF
LITL
PLW
IV-
NVA
GCLF
FIN
TYCS
VAF-
LGVI
-----I
SLSL
VIW
VAIV
GAAS
YFLI
L--IH
IFIV
FSFF
LVFL
IILFC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
-VTL
CLLS
TNCV
LDPV
IYCF
LT--
Chic
ken
--TVF
YSIIF
ILGF
VAN
CYVL
WI-
--FM
LNLT
VADL
LFLV
TMPL
WIV
-SV
AGSL
FFVN
TYAS
VAF-
LMVI
----G
IFLS
AAIW
IITVG
SALY
YIF-
-AI
HIIIC
TLFY
VIFL
FILT
WN
---RA
LWM
VCTV
LVVF
IISFV
PHH-
----
-VTL
CLLS
MN
CVLD
PVIY
CFLT
--Ch
impa
nzee
-FPI
VYSI
IFVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
-N
VAGC
LFFI
NTY
CSVA
F-LG
VI---
--ISL
SLVI
WVA
IVGA
ASYF
LIL-
-IHIF
IVFS
FFLV
FLIIL
FCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
--V
TLCL
LSTN
CVLD
PVIY
CFLT
--Co
w-F
PIFY
SIVF
VLGV
IAN
SYVL
W--
---M
VNLT
MAD
LLFL
VTLP
LWIV
YN
LAGC
FFFI
NTY
CSVA
F-LA
VI---
--ILL
SLIIW
VSIV
GAAS
YFFV
L--IH
IFLV
FSFF
LVFL
IILFC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
-VTL
CLLS
TNCV
LDPI
IYCF
LT--
Elep
hant
-FPI
FYSI
IFVL
GVIA
NGY
VLW
-----
MVN
LTM
ADLL
FLVT
LPLW
IVY
NLA
GCLF
FIN
TYCS
VAF-
LGVI
-----I
TLSL
VIW
VTIV
AAAS
YFLF
M-
--HIF
IVCS
FFLV
FFIIL
VCN
LV-
RALW
MVC
TVLA
VFIIC
FVPH
H----
-Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Frog
---VT
YSIIF
VFGF
IAN
CYVL
WIF
-IFM
INLT
IADL
LFLV
TLPL
WI--
NVA
GCFF
FLN
TYCS
VAF-
LGVI
------
CISV
AVW
ILLF
ASSL
YFLI
FP--H
FILV
GAFF
VVFL
IILTC
NLV
-RA
LWM
VVTV
LAVF
VLCF
VPHH
-----
---LC
LMST
NCM
LDPI
IYCF
LTKK
Goat
-FPI
FYSI
VFVL
GVIA
NSY
VLW
-----
MVN
LTM
ADLL
FLVT
LPLW
IVY
NLA
GCFF
FIN
TYCS
VAF-
LAVI
-----F
LLSL
IIWVS
IVGA
ASYF
FVL-
-IHIF
LVFS
FFLV
FLIIL
FCN
L--
RALW
MVC
TVLA
VFVI
CFVP
HH---
---V
TLCL
LSTN
CVLD
PIIY
CFLT
--Gu
inea
pig
-FPI
VYSI
IFVL
GIIA
NGY
VLW
----F
MVN
LTVA
DLLF
LITL
PLW
IV-
NLA
GCLF
FIN
TYCS
VAF-
LGVI
-----I
ALSL
VIW
VAIV
AAAS
YFLV
M-
-IHIC
IVLG
FFIV
FLLI
LFCN
L--
RALW
MVC
TVLA
VFVI
CFVP
HH---
--Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Hedg
ehog
-FPI
FYSI
IFAL
GVIA
NSY
VLW
----F
MVN
LTLA
DLLF
LVTL
PLW
II-N
LAGC
FFFI
NTY
CSVA
F-LG
VI---
-GIIL
SLVI
WVA
IVSA
ASYF
FF--
-IHIF
LVVS
FFVV
FLIIF
FCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
-Q
ITLC
LLST
NCV
LDPI
IYCF
L---
Hors
e--P
IVYS
IIFVL
GVIA
NSY
VLW
V--IF
MVN
LTVA
DLLF
LLTL
PLW
V--
NLT
GCCF
FIN
TYCS
VAF-
LGVI
-----I
SLSL
VIW
LAIV
AAAS
YFLF
M-
-IHIF
LVFS
FFLV
FLIIL
VCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
-Q
VTLC
LLST
NCV
LDPI
IYCF
L---
Med
aka
-FSV
VYSI
FFVV
GLLA
NVY
VLF-
---Y
MTN
LTIA
DLLF
VCAL
PFW
IG-
--TGS
FFFI
NTY
GTIL
F-LG
AISL
-RG
IIVSV
LIW
LCLV
SSAI
PSL-
----H
FLIIG
LFFV
VFFL
VMVC
NLL
----
QM
LLAV
VGVF
LLCF
LPHH
VVQ
--Q
ITLV
LMGL
NCI
LDPV
IYYF
A---
Mon
key
-FPI
VYSI
IFVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
-N
LAGC
LFFI
NTY
CSVA
F-LG
VI---
--ISL
SLVI
WVA
IVGA
ASYF
FIL-
-IHIF
IVFS
FFLV
FLIIL
FCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
-Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Mou
se--F
MVN
LTM
ADLL
FLIT
LPLW
IV-
-FPI
VYSV
IFIL
GVVA
NGY
VLW
--N
VAGC
LFFI
NTY
CSVA
F-LG
VI---
-GIS
LSLI
IWVS
IVAT
ASYF
LA--
VVHV
FIAF
CFFL
VFFL
IFYC
N---
RALW
MVC
TVLA
VFIIC
FVPH
H----
--IT
LCLL
STN
CVLD
PVIY
CFLT
--O
poss
um-F
PIVY
SIIF
VLGF
VSN
AYVL
W--
---M
VNLT
VADL
LFLF
ALPL
WIV
YN
VAGC
LFFI
NTY
CSVC
F-LG
VI---
--HVF
IVLC
FFIV
FLVI
LVCN
VI-
-GIL
LSTI
IWVV
IVGC
ASYF
LI--
--LW
MVC
TVM
AVFF
ICFV
PHHV
V---
QVT
LCLM
SIN
CVLD
PIIY
CFL-
--O
rang
utan
-FPI
VYSI
IFVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
-N
VAGC
LFFI
NTY
CSVA
F-LG
VI---
--ISL
SLVI
WVA
IVGA
ASYF
LIL-
-IHIF
IVFS
FFLV
FLIIL
FCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
--V
TLCL
LSTN
CVLD
PVIY
CFLT
--Pi
g-F
PIFY
SIIF
VLGV
IAN
SYVL
W--
NLA
GCFF
FIN
TYCS
VSF-
LAVI
------
MLN
LTM
ADLL
FLVT
LPLW
IIY--I
SLSL
IIWVA
MVA
AASY
FFVL
--IH
IFLV
FSFF
LVFL
VILF
CNL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
-ITLC
LLST
NCV
LDPI
IYCF
LT--
Pika
-FPI
VYSI
IFVL
GVVA
NGY
VLW
----F
MVN
LTVA
DLLF
LITL
PLW
II-N
VAGC
LFFI
NTY
CSVA
F-LG
VI---
--ISL
SLIIW
VVIL
AIAS
YFLI
M-
-IHIF
IVFC
FFVV
FLIIL
VCN
L--
QVT
LCLL
STN
CVLD
PVIY
CFL-
--RA
LWM
VCTV
LGVF
IICFV
PHH-
----
Plat
ypus
---VA
YSIIF
VLGI
FAN
GYVL
WVF
--FM
VNLT
VADL
LFLV
TMPL
WIV
-N
VAGC
LFFI
NTY
CSVA
F-LG
VI---
-GIV
LSLV
IWVL
IVSS
ALYF
LV--
-IHIF
IVAS
FFAV
FLLI
LVCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
--V
TLCL
LSTN
CVLD
PVIY
CFLT
--Pu
fferfish
--PSA
YGVI
FILG
LLAN
LYVL
FI-
---M
TNLS
IADL
LFVC
ALPF
WIG
Y---
GSLF
FIN
TYCS
VLF-
LGAI
SVN
-GIII
SVIIW
VLTI
SMAI
PYLV
-----
FAIIG
LFFV
VFAL
VLVC
NLL
I---
--LLA
VVGV
FVVC
FLPH
HVIQ
GAQ
ITLL
LMGL
NCI
LDPV
VYCF
A---
Rabb
it--P
IVYS
IIFVL
GVVA
NGY
VLW
V---F
MVN
LTVA
DLLF
LITL
PLW
II-N
LAGC
LFFI
NTY
CSVA
F-LG
VI---
--IAL
SLVI
WVS
ISAA
ATYF
LIL-
-IHIF
IVAS
FFIV
FLIIL
ICN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
-Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Rat
--PIV
YSVI
FVLG
VVAN
GYVL
WV-
--FM
VNLT
VADL
LFLM
TLPL
WIV
-N
LAGC
LFFI
NTY
CSVA
F-LG
VI---
-GIT
LSLV
IWIS
IAAT
ASYF
LA--
-VHI
FITS
CFFL
VFFL
IFYC
NM
--RA
LWM
VCTV
LAVF
VICF
VPHH
-----
-ITLC
LLST
NCV
LDPV
IYCF
LT--
Shre
w-F
PIFY
SIIF
VLGV
IAN
SYVL
W--
--FM
VNLT
MAD
LLFL
ITLP
LWIV
-N
LTGC
FFFI
NTY
CSVA
F-LG
VI---
-GIY
LSLV
IWM
AIVS
AASY
FFF-
--IH
IFLV
CSFF
LVFL
LIFF
CNL-
-RA
LWM
VCTV
LAVF
SICF
VPHH
-----
-VTL
CLLS
TNCV
LDPI
IYCF
LT--
Squi
rrel
LFPI
IYSI
IFVV
GVIA
NGY
VL---
--FM
LNLT
VADL
LFLI
TLPL
WII-
NVA
GCLF
FIN
TYCS
VAF-
LGVI
------
YLSL
VIW
VFIV
AAAS
YFLI
LDVI
HIFI
VFSF
FLVF
LIIL
FCK-
--RA
LWM
VCAV
LAVF
VICF
VPHH
-----
QVT
LCLL
STN
CVLD
PVIY
CFL-
--Sti
ckleba
ck-F
PVVY
SIIF
ILGI
TAN
LYVL
F--
CIYM
INLT
IGDL
LFVT
FLPF
W---
--SGS
LFFI
NTY
SSVL
F-LC
AISV
--G
IILSV
FIW
LFTL
ATAI
LFLI
----H
LTIV
IGFF
LVFF
VVVV
CNFL
----
QM
LFSV
VGVF
VVCF
LPHH
ACQ
--Q
ITW
LLM
SLN
CILD
PVVY
FFA-
--Zebrafi
sh-IY
MTN
LTVA
DLLF
VSAL
PFW
I----P
IFYG
VVFV
LGLI
ANCY
ALYV
----
GTFF
FIN
TYGS
ILF-
LTVI
SIN
RGAI
ISVL
IWAF
ILSA
SIPQ
L---
--LRM
LCAV
IGVF
VICF
LPHH
VV---
ITHF
SIIG
LFFL
VFLM
VIIC
N---
QIT
LMLM
GIN
CLLD
PLVY
CFA-
--
Sequ
ence
Dom
ain
ID
omai
n II
Dom
ain
III
Dom
ain
IVD
omai
n V
Dom
ain
VI
Dom
ain
VII
Tc00
.104
7053
5087
23.2
4GF
YFGS
TVVA
FLAL
LL--F
FIFL
-AV
PLFV
LVIV
ALLI
TAFA
AIV-
----V
GNFI
AVVC
YTIF
CFLV
VSAV
--A
AYFS
LPIIA
DVV-
---YY
LSSL
VW---
--LCY
GFFF
LGLI
YPCI
SIAQ
WL-
-----T
FDTL
GAFF
FNGM
LLVS
LYFV
---LI
PVYA
ALLY
LLCA
M---
--FFT
VVY
Hum
an-F
PIVY
SIIF
VLGV
IAN
GYVL
W--
--FM
VNLT
MAD
MLF
LITL
PLW
IV-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
---I
SLSL
VIW
VAIV
GAAS
YFLI
L---
-IHIF
IVFS
FF---
LVFL
IILFC
NL-
-RA
LWM
VCTV
LAVF
II---C
FVPH
H----
----
VTLC
LLST
NCV
LDPV
IYCF
LT--
Chic
ken
--TVF
YSIIF
ILGF
VAN
CYVL
WI-
--FM
LNLT
VADL
LFLV
TMPL
WIV
-SV
AGSL
FFVN
TYAS
VAFL
MVI
----G
IFLS
AAIW
IITVG
SALY
YIF-
---AI
HIIIC
TLFY
---VI
FLFI
LTW
N---
RALW
MVC
TVLV
VFII-
--SFV
PHH-
----
---VT
LCLL
SMN
CVLD
PVIY
CFLT
--Ch
impa
nzee
-FPI
VYSI
IFVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLVI
WVA
IVGA
ASYF
LIL-
---IH
IFIV
FSFF
---LV
FLIIL
FCN
L--
RALW
MVC
TVLA
VFII-
--CFV
PHH-
----
---VT
LCLL
STN
CVLD
PVIY
CFLT
--Co
w-F
PIFY
SIVF
VLGV
IAN
SYVL
W--
---M
VNLT
MAD
LLFL
VTLP
LWIV
YN
LAGC
FFFI
NTY
CSVA
FLAV
I---
--ILL
SLIIW
VSIV
GAAS
YFFV
L---
-IHIF
LVFS
FF---
LVFL
IILFC
NL-
-RA
LWM
VCTV
LAVF
II---C
FVPH
H----
----
VTLC
LLST
NCV
LDPI
IYCF
LT--
Elep
hant
-FPI
FYSI
IFVL
GVIA
NGY
VLW
-----
MVN
LTM
ADLL
FLVT
LPLW
IVY
NLA
GCLF
FIN
TYCS
VAFL
GVI--
---I
TLSL
VIW
VTIV
AAAS
YFLF
M---
--HIF
IVCS
FF---
LVFF
IILVC
NLV
-RA
LWM
VCTV
LAVF
II---C
FVPH
H----
---Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Frog
---VT
YSIIF
VFGF
IAN
CYVL
WIF
-IFM
INLT
IADL
LFLV
TLPL
WI--
NVA
GCFF
FLN
TYCS
VAFL
GVI--
----
CISV
AVW
ILLF
ASSL
YFLI
FP--
--HFI
LVGA
FF---
VVFL
IILTC
NLV
-RA
LWM
VVTV
LAVF
VL---
CFVP
HH---
-----
--LCL
MST
NCM
LDPI
IYCF
LTKK
Goat
-FPI
FYSI
VFVL
GVIA
NSY
VLW
-----
MVN
LTM
ADLL
FLVT
LPLW
IVY
NLA
GCFF
FIN
TYCS
VAFL
AVI--
--IH
IFLV
FSFF
---LV
FLIIL
FCN
L--
--FLL
SLIIW
VSIV
GAAS
YFFV
L---
RALW
MVC
TVLA
VFVI
---CF
VPHH
-----
---VT
LCLL
STN
CVLD
PIIY
CFLT
--Gu
inea
pig
-FPI
VYSI
IFVL
GIIA
NGY
VLW
----F
MVN
LTVA
DLLF
LITL
PLW
IV-
NLA
GCLF
FIN
TYCS
VAFL
GVI--
---I
ALSL
VIW
VAIV
AAAS
YFLV
M---
-IHIC
IVLG
FF---
IVFL
LILF
CNL-
-RA
LWM
VCTV
LAVF
VI---
CFVP
HH---
----Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Hedg
ehog
-FPI
FYSI
IFAL
GVIA
NSY
VLW
----F
MVN
LTLA
DLLF
LVTL
PLW
II-N
LAGC
FFFI
NTY
CSVA
FLGV
I---
-GIIL
SLVI
WVA
IVSA
ASYF
FF---
--IH
IFLV
VSFF
---VV
FLIIF
FCN
L--
RALW
MVC
TVLA
VFII-
--CFV
PHH-
----
--QIT
LCLL
STN
CVLD
PIIY
CFL-
--Ho
rse
--PIV
YSIIF
VLGV
IAN
SYVL
WV-
-IFM
VNLT
VADL
LFLL
TLPL
WV-
-N
LTGC
CFFI
NTY
CSVA
FLGV
I---
--ISL
SLVI
WLA
IVAA
ASYF
LFM
----IH
IFLV
FSFF
---LV
FLIIL
VCN
L--
RALW
MVC
TVLA
VFII-
--CFV
PHH-
----
--QVT
LCLL
STN
CVLD
PIIY
CFL-
--M
edak
a-F
SVVY
SIFF
VVGL
LAN
VYVL
F--
--YM
TNLT
IADL
LFVC
ALPF
WIG
---T
GSFF
FIN
TYGT
ILFL
GAIS
L-RG
IIVSV
LIW
LCLV
SSAI
PSL-
----
--HFL
IIGLF
F---V
VFFL
VMVC
NLL
----
QM
LLAV
VGVF
LL---
CFLP
HHVV
Q--
--QIT
LVLM
GLN
CILD
PVIY
YFA-
--M
onke
y-F
PIVY
SIIF
VLGV
IAN
GYVL
W--
--FM
VNLT
MAD
MLF
LITL
PLW
IV-
NLA
GCLF
FIN
TYCS
VAFL
GVI--
---I
SLSL
VIW
VAIV
GAAS
YFFI
L---
-IHIF
IVFS
FF---
LVFL
IILFC
NL-
-RA
LWM
VCTV
LAVF
II---C
FVPH
H----
---Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Mou
se-F
PIVY
SVIF
ILGV
VAN
GYVL
W--
--FM
VNLT
MAD
LLFL
ITLP
LWIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
-GIS
LSLI
IWVS
IVAT
ASYF
LA---
-VV
HVFI
AFCF
F---L
VFFL
IFYC
N---
RALW
MVC
TVLA
VFII-
--CFV
PHH-
----
---IT
LCLL
STN
CVLD
PVIY
CFLT
--O
poss
um-F
PIVY
SIIF
VLGF
VSN
AYVL
W--
---M
VNLT
VADL
LFLF
ALPL
WIV
YN
VAGC
LFFI
NTY
CSVC
FLGV
I---
-GIL
LSTI
IWVV
IVGC
ASYF
LI---
---H
VFIV
LCFF
---IV
FLVI
LVCN
VI-
--LW
MVC
TVM
AVFF
I---C
FVPH
HVV-
----Q
VTLC
LMSI
NCV
LDPI
IYCF
L---
Ora
ngut
an-F
PIVY
SIIF
VLGV
IAN
GYVL
W--
--FM
VNLT
MAD
MLF
LITL
PLW
IV-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
---I
SLSL
VIW
VAIV
GAAS
YFLI
L---
-IHIF
IVFS
FF---
LVFL
IILFC
NL-
-RA
LWM
VCTV
LAVF
II---C
FVPH
H----
----
VTLC
LLST
NCV
LDPV
IYCF
LT--
Pig
-FPI
FYSI
IFVL
GVIA
NSY
VLW
-----
MLN
LTM
ADLL
FLVT
LPLW
IIYN
LAGC
FFFI
NTY
CSVS
FLAV
I---
--ISL
SLIIW
VAM
VAAA
SYFF
VL---
-IHIF
LVFS
FF---
LVFL
VILF
CNL-
-RA
LWM
VCTV
LAVF
II---C
FVPH
H----
----
ITLC
LLST
NCV
LDPI
IYCF
LT--
Pika
-FPI
VYSI
IFVL
GVVA
NGY
VLW
----F
MVN
LTVA
DLLF
LITL
PLW
II-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLIIW
VVIL
AIAS
YFLI
M---
-IHIF
IVFC
FF---
VVFL
IILVC
NL-
-RA
LWM
VCTV
LGVF
II---C
FVPH
H----
---Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Plat
ypus
---VA
YSIIF
VLGI
FAN
GYVL
WVF
--FM
VNLT
VADL
LFLV
TMPL
WIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
-GIV
LSLV
IWVL
IVSS
ALYF
LV---
--IH
IFIV
ASFF
---AV
FLLI
LVCN
L--
RALW
MVC
TVLA
VFII-
--CFV
PHH-
----
---VT
LCLL
STN
CVLD
PVIY
CFLT
--Pu
fferfish
--PSA
YGVI
FILG
LLAN
LYVL
FI-
---M
TNLS
IADL
LFVC
ALPF
WIG
Y---
GSLF
FIN
TYCS
VLFL
GAIS
VN-G
IIISV
IIWVL
TISM
AIPY
LV---
----
FAIIG
LFF-
--VVF
ALVL
VCN
LLI
-----L
LAVV
GVFV
V---C
FLPH
HVIQ
GA--Q
ITLL
LMGL
NCI
LDPV
VYCF
A---
Rabb
it--P
IVYS
IIFVL
GVVA
NGY
VLW
V---F
MVN
LTVA
DLLF
LITL
PLW
II-N
LAGC
LFFI
NTY
CSVA
FLGV
I---
--IAL
SLVI
WVS
ISAA
ATYF
LIL-
---IH
IFIV
ASFF
---IV
FLIIL
ICN
L--
RALW
MVC
TVLA
VFII-
--CFV
PHH-
----
--QVT
LCLL
STN
CVLD
PVIY
CFL-
--Ra
t--P
IVYS
VIFV
LGVV
ANGY
VLW
V---F
MVN
LTVA
DLLF
LMTL
PLW
IV-
NLA
GCLF
FIN
TYCS
VAFL
GVI--
--G
ITLS
LVIW
ISIA
ATAS
YFLA
----
-VHI
FITS
CFF-
--LVF
FLIF
YCN
M--
RALW
MVC
TVLA
VFVI
---CF
VPHH
-----
---IT
LCLL
STN
CVLD
PVIY
CFLT
--Sh
rew
-FPI
FYSI
IFVL
GVIA
NSY
VLW
----F
MVN
LTM
ADLL
FLIT
LPLW
IV-
NLT
GCFF
FIN
TYCS
VAFL
GVI--
--G
IYLS
LVIW
MAI
VSAA
SYFF
F----
-IHIF
LVCS
FF---
LVFL
LIFF
CNL-
-RA
LWM
VCTV
LAVF
SI---
CFVP
HH---
-----
VTLC
LLST
NCV
LDPI
IYCF
LT--
Squi
rrel
LFPI
IYSI
IFVV
GVIA
NGY
VL---
--FM
LNLT
VADL
LFLI
TLPL
WII-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
----
YLSL
VIW
VFIV
AAAS
YFLI
LD--
VIHI
FIVF
SFF-
--LVF
LIIL
FCK-
--RA
LWM
VCAV
LAVF
VI---
CFVP
HH---
----Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Stickleba
ck-F
PVVY
SIIF
ILGI
TAN
LYVL
F--
CIYM
INLT
IGDL
LFVT
FLPF
W---
--SGS
LFFI
NTY
SSVL
FLCA
ISV-
-GIIL
SVFI
WLF
TLAT
AILF
LI---
---H
LTIV
IGFF
---LV
FFVV
VVCN
FL-
---Q
MLF
SVVG
VFVV
---CF
LPHH
ACQ
----Q
ITW
LLM
SLN
CILD
PVVY
FFA-
--Zebrafi
sh--P
IFYG
VVFV
LGLI
ANCY
ALYV
--IY
MTN
LTVA
DLLF
VSAL
PFW
I-----
GTFF
FIN
TYGS
ILFL
TVIS
INRG
AIIS
VLIW
AFIL
SASI
PQL-
----
--LRM
LCAV
IGVF
VI---
CFLP
HHVV
---IT
HFSI
IGLF
F---L
VFLM
VIIC
N---
--QIT
LMLM
GIN
CLLD
PLVY
CFA-
--
4Supplementary data Search for PAF receptor in T. cruzi • Daniel Fábio Kawano et al.
Sequ
ence
Dom
ain
ID
omai
n II
Dom
ain
III
Dom
ain
IVD
omai
n V
Dom
ain
VI
Dom
ain
VII
Tc00
.104
7053
5097
91.6
0GF
YFGS
TVVA
FLAL
LL--F
FIFL
-AA
PLFV
LVIV
ALLI
TAFA
AIV-
----V
GNFI
AVVC
YTIF
CFLV
VSAV
--A
AYFS
LPIIA
DVV-
---YY
LSSL
VW---
--LCY
GFFF
LGLI
YPCI
SIAQ
WL-
-----T
FDTL
GAFF
FNGM
LLVS
LYFV
---LI
PVYA
ALLY
LLCA
M---
--FFT
VVY
Hum
an-F
PIVY
SIIF
VLGV
IAN
GYVL
W--
--FM
VNLT
MAD
MLF
LITL
PLW
IV-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
---I
SLSL
VIW
VAIV
GAAS
YFLI
L---
-IHIF
IVFS
FF---
LVFL
IILFC
NL-
-RA
LWM
VCTV
LAVF
II---C
FVPH
H----
----
VTLC
LLST
NCV
LDPV
IYCF
LT--
Chic
ken
--TVF
YSIIF
ILGF
VAN
CYVL
WI-
--FM
LNLT
VADL
LFLV
TMPL
WIV
-SV
AGSL
FFVN
TYAS
VAFL
MVI
----G
IFLS
AAIW
IITVG
SALY
YIF-
---RA
LWM
VCTV
LVVF
II---S
FVPH
H----
-AI
HIIIC
TLFY
---VI
FLFI
LTW
N---
---VT
LCLL
SMN
CVLD
PVIY
CFLT
--Ch
impa
nzee
-FPI
VYSI
IFVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLVI
WVA
IVGA
ASYF
LIL-
---IH
IFIV
FSFF
---LV
FLIIL
FCN
L--
---VT
LCLL
STN
CVLD
PVIY
CFLT
--RA
LWM
VCTV
LAVF
II---C
FVPH
H----
-Co
w-F
PIFY
SIVF
VLGV
IAN
SYVL
W--
---M
VNLT
MAD
LLFL
VTLP
LWIV
YN
LAGC
FFFI
NTY
CSVA
FLAV
I---
--ILL
SLIIW
VSIV
GAAS
YFFV
L---
-IHIF
LVFS
FF---
LVFL
IILFC
NL-
-RA
LWM
VCTV
LAVF
II---C
FVPH
H----
----
VTLC
LLST
NCV
LDPI
IYCF
LT--
Elep
hant
-FPI
FYSI
IFVL
GVIA
NGY
VLW
-----
MVN
LTM
ADLL
FLVT
LPLW
IVY
NLA
GCLF
FIN
TYCS
VAFL
GVI--
---I
TLSL
VIW
VTIV
AAAS
YFLF
M---
--HIF
IVCS
FF---
LVFF
IILVC
NLV
-RA
LWM
VCTV
LAVF
II---C
FVPH
H----
---Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Frog
---VT
YSIIF
VFGF
IAN
CYVL
WIF
-IFM
INLT
IADL
LFLV
TLPL
WI--
NVA
GCFF
FLN
TYCS
VAFL
GVI--
----
CISV
AVW
ILLF
ASSL
YFLI
FP--
--HFI
LVGA
FF---
VVFL
IILTC
NLV
-RA
LWM
VVTV
LAVF
VL---
CFVP
HH---
-----
--LCL
MST
NCM
LDPI
IYCF
LTKK
Goat
-FPI
FYSI
VFVL
GVIA
NSY
VLW
-----
MVN
LTM
ADLL
FLVT
LPLW
IVY
NLA
GCFF
FIN
TYCS
VAFL
AVI--
---F
LLSL
IIWVS
IVGA
ASYF
FVL-
---IH
IFLV
FSFF
---LV
FLIIL
FCN
L--
RALW
MVC
TVLA
VFVI
---CF
VPHH
-----
---VT
LCLL
STN
CVLD
PIIY
CFLT
--Gu
inea
pig
-FPI
VYSI
IFVL
GIIA
NGY
VLW
----F
MVN
LTVA
DLLF
LITL
PLW
IV-
NLA
GCLF
FIN
TYCS
VAFL
GVI--
---I
ALSL
VIW
VAIV
AAAS
YFLV
M---
-IHIC
IVLG
FF---
IVFL
LILF
CNL-
-RA
LWM
VCTV
LAVF
VI---
CFVP
HH---
----Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Hedg
ehog
-FPI
FYSI
IFAL
GVIA
NSY
VLW
----F
MVN
LTLA
DLLF
LVTL
PLW
II-N
LAGC
FFFI
NTY
CSVA
FLGV
I---
-GIIL
SLVI
WVA
IVSA
ASYF
FF---
--IH
IFLV
VSFF
---VV
FLIIF
FCN
L--
RALW
MVC
TVLA
VFII-
--CFV
PHH-
----
--QIT
LCLL
STN
CVLD
PIIY
CFL-
--Ho
rse
--PIV
YSIIF
VLGV
IAN
SYVL
WV-
-IFM
VNLT
VADL
LFLL
TLPL
WV-
-N
LTGC
CFFI
NTY
CSVA
FLGV
I---
--ISL
SLVI
WLA
IVAA
ASYF
LFM
----IH
IFLV
FSFF
---LV
FLIIL
VCN
L--
RALW
MVC
TVLA
VFII-
--CFV
PHH-
----
--QVT
LCLL
STN
CVLD
PIIY
CFL-
--M
edak
a-F
SVVY
SIFF
VVGL
LAN
VYVL
F--
--YM
TNLT
IADL
LFVC
ALPF
WIG
---T
GSFF
FIN
TYGT
ILFL
GAIS
L-RG
IIVSV
LIW
LCLV
SSAI
PSL-
----
--HFL
IIGLF
F---V
VFFL
VMVC
NLL
----
QM
LLAV
VGVF
LL---
CFLP
HHVV
Q--
--QIT
LVLM
GLN
CILD
PVIY
YFA-
--M
onke
y-F
PIVY
SIIF
VLGV
IAN
GYVL
W--
--FM
VNLT
MAD
MLF
LITL
PLW
IV-
NLA
GCLF
FIN
TYCS
VAFL
GVI--
---I
SLSL
VIW
VAIV
GAAS
YFFI
L---
-IHIF
IVFS
FF---
LVFL
IILFC
NL-
---Q
VTLC
LLST
NCV
LDPV
IYCF
L---
RALW
MVC
TVLA
VFII-
--CFV
PHH-
----
Mou
se-F
PIVY
SVIF
ILGV
VAN
GYVL
W--
--FM
VNLT
MAD
LLFL
ITLP
LWIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
-GIS
LSLI
IWVS
IVAT
ASYF
LA---
-VV
HVFI
AFCF
F---L
VFFL
IFYC
N---
RALW
MVC
TVLA
VFII-
--CFV
PHH-
----
---IT
LCLL
STN
CVLD
PVIY
CFLT
--O
poss
um-F
PIVY
SIIF
VLGF
VSN
AYVL
W--
---M
VNLT
VADL
LFLF
ALPL
WIV
YN
VAGC
LFFI
NTY
CSVC
FLGV
I---
-GIL
LSTI
IWVV
IVGC
ASYF
LI---
---H
VFIV
LCFF
---IV
FLVI
LVCN
VI-
--LW
MVC
TVM
AVFF
I---C
FVPH
HVV-
----Q
VTLC
LMSI
NCV
LDPI
IYCF
L---
Ora
ngut
an-F
PIVY
SIIF
VLGV
IAN
GYVL
W--
--FM
VNLT
MAD
MLF
LITL
PLW
IV-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
---I
SLSL
VIW
VAIV
GAAS
YFLI
L---
-IHIF
IVFS
FF---
LVFL
IILFC
NL-
-RA
LWM
VCTV
LAVF
II---C
FVPH
H----
----
VTLC
LLST
NCV
LDPV
IYCF
LT--
Pig
-FPI
FYSI
IFVL
GVIA
NSY
VLW
-----
MLN
LTM
ADLL
FLVT
LPLW
IIYN
LAGC
FFFI
NTY
CSVS
FLAV
I---
-IHIF
LVFS
FF---
LVFL
VILF
CNL-
---I
SLSL
IIWVA
MVA
AASY
FFVL
---RA
LWM
VCTV
LAVF
II---C
FVPH
H----
----
ITLC
LLST
NCV
LDPI
IYCF
LT--
Pika
-FPI
VYSI
IFVL
GVVA
NGY
VLW
----F
MVN
LTVA
DLLF
LITL
PLW
II-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLIIW
VVIL
AIAS
YFLI
M---
-IHIF
IVFC
FF---
VVFL
IILVC
NL-
-RA
LWM
VCTV
LGVF
II---C
FVPH
H----
---Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Plat
ypus
---VA
YSIIF
VLGI
FAN
GYVL
WVF
--FM
VNLT
VADL
LFLV
TMPL
WIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
-GIV
LSLV
IWVL
IVSS
ALYF
LV---
--IH
IFIV
ASFF
---AV
FLLI
LVCN
L--
RALW
MVC
TVLA
VFII-
--CFV
PHH-
----
---VT
LCLL
STN
CVLD
PVIY
CFLT
--Pu
fferfish
--PSA
YGVI
FILG
LLAN
LYVL
FI-
---M
TNLS
IADL
LFVC
ALPF
WIG
Y---
GSLF
FIN
TYCS
VLFL
GAIS
VN-G
IIISV
IIWVL
TISM
AIPY
LV---
----
FAIIG
LFF-
--VVF
ALVL
VCN
LLI
-----L
LAVV
GVFV
V---C
FLPH
HVIQ
GA--Q
ITLL
LMGL
NCI
LDPV
VYCF
A---
Rabb
it--P
IVYS
IIFVL
GVVA
NGY
VLW
V---F
MVN
LTVA
DLLF
LITL
PLW
II-N
LAGC
LFFI
NTY
CSVA
FLGV
I---
--IAL
SLVI
WVS
ISAA
ATYF
LIL-
---IH
IFIV
ASFF
---IV
FLIIL
ICN
L--
RALW
MVC
TVLA
VFII-
--CFV
PHH-
----
--QVT
LCLL
STN
CVLD
PVIY
CFL-
--Ra
t--P
IVYS
VIFV
LGVV
ANGY
VLW
V---F
MVN
LTVA
DLLF
LMTL
PLW
IV-
NLA
GCLF
FIN
TYCS
VAFL
GVI--
--G
ITLS
LVIW
ISIA
ATAS
YFLA
----
-VHI
FITS
CFF-
--LVF
FLIF
YCN
M--
RALW
MVC
TVLA
VFVI
---CF
VPHH
-----
---IT
LCLL
STN
CVLD
PVIY
CFLT
--Sh
rew
-FPI
FYSI
IFVL
GVIA
NSY
VLW
----F
MVN
LTM
ADLL
FLIT
LPLW
IV-
NLT
GCFF
FIN
TYCS
VAFL
GVI--
--G
IYLS
LVIW
MAI
VSAA
SYFF
F----
-IHIF
LVCS
FF---
LVFL
LIFF
CNL-
-RA
LWM
VCTV
LAVF
SI---
CFVP
HH---
-----
VTLC
LLST
NCV
LDPI
IYCF
LT--
Squi
rrel
LFPI
IYSI
IFVV
GVIA
NGY
VL---
--FM
LNLT
VADL
LFLI
TLPL
WII-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
----
YLSL
VIW
VFIV
AAAS
YFLI
LD--
VIHI
FIVF
SFF-
--LVF
LIIL
FCK-
--RA
LWM
VCAV
LAVF
VI---
CFVP
HH---
----Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Stickleba
ck-F
PVVY
SIIF
ILGI
TAN
LYVL
F--
CIYM
INLT
IGDL
LFVT
FLPF
W---
--SGS
LFFI
NTY
SSVL
FLCA
ISV-
-GIIL
SVFI
WLF
TLAT
AILF
LI---
---H
LTIV
IGFF
---LV
FFVV
VVCN
FL-
---Q
MLF
SVVG
VFVV
---CF
LPHH
ACQ
----Q
ITW
LLM
SLN
CILD
PVVY
FFA-
--Zebrafi
sh--P
IFYG
VVFV
LGLI
ANCY
ALYV
--IY
MTN
LTVA
DLLF
VSAL
PFW
I-----
GTFF
FIN
TYGS
ILFL
TVIS
INRG
AIIS
VLIW
AFIL
SASI
PQL-
----
ITHF
SIIG
LFF-
--LVF
LMVI
ICN
-----L
RMLC
AVIG
VFVI
---CF
LPHH
VV---
--QIT
LMLM
GIN
CLLD
PLVY
CFA-
--
Sequ
ence
Dom
ain
ID
omai
n II
Dom
ain
III
Dom
ain
IVD
omai
n V
Dom
ain
VI
Dom
ain
VII
Tc00
.104
7053
5067
53.3
0-L
EMVF
FIN
IVGC
LVPG
GACL
L--
RLFF
VVN
FVN
VLAT
YLM
FSSF
----
------
VSSS
TWVS
VFFV
CIGA
SLTS
G-S
LGAA
VIM
FTYI
LVFA
SNAA
L--
---SC
LVHF
FVLL
LSSI
TLLG
PVL
-----Q
IVVV
GISY
GVYQ
VFN
GVVL
FDS
VLQ
LLM
GLIL
LFAG
SYCY
V---
Hum
an-F
PIVY
SIIF
VLGV
IAN
GYVL
W--
---FM
VNLT
MAD
MLF
LITL
PLW
IV-
NVA
GCLF
FIN
TYCS
VAFL
GV---
I---
--ISL
SLVI
WVA
IVGA
ASYF
LIL-
-IHIF
IVFS
FFLV
FLIIL
FCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
--V
TLCL
LSTN
CVLD
PVIY
CFLT
--Ch
icke
n--T
VFYS
IIFIL
GFVA
NCY
VLW
I----
FMLN
LTVA
DLLF
LVTM
PLW
IV-
SVAG
SLFF
VNTY
ASVA
FLM
V---I
----G
IFLS
AAIW
IITVG
SALY
YIF-
-AI
HIIIC
TLFY
VIFL
FILT
WN
---RA
LWM
VCTV
LVVF
IISFV
PHH-
----
-VTL
CLLS
MN
CVLD
PVIY
CFLT
--Ch
impa
nzee
-FPI
VYSI
IFVL
GVIA
NGY
VLW
-----
FMVN
LTM
ADM
LFLI
TLPL
WIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
---I--
---I
SLSL
VIW
VAIV
GAAS
YFLI
L--IH
IFIV
FSFF
LVFL
IILFC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
-VTL
CLLS
TNCV
LDPV
IYCF
LT--
Cow
-FPI
FYSI
VFVL
GVIA
NSY
VLW
-----
-MVN
LTM
ADLL
FLVT
LPLW
IVY
NLA
GCFF
FIN
TYCS
VAFL
AV---
I---
--ILL
SLIIW
VSIV
GAAS
YFFV
L--IH
IFLV
FSFF
LVFL
IILFC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
-VTL
CLLS
TNCV
LDPI
IYCF
LT--
Elep
hant
-FPI
FYSI
IFVL
GVIA
NGY
VLW
-----
-MVN
LTM
ADLL
FLVT
LPLW
IVY
NLA
GCLF
FIN
TYCS
VAFL
GV---
I---
--ITL
SLVI
WVT
IVAA
ASYF
LFM
---H
IFIV
CSFF
LVFF
IILVC
NLV
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
QVT
LCLL
STN
CVLD
PVIY
CFL-
--Fr
og---
VTYS
IIFVF
GFIA
NCY
VLW
IF--I
FMIN
LTIA
DLLF
LVTL
PLW
I--N
VAGC
FFFL
NTY
CSVA
FLGV
---I--
----
CISV
AVW
ILLF
ASSL
YFLI
FP--H
FILV
GAFF
VVFL
IILTC
NLV
-RA
LWM
VVTV
LAVF
VLCF
VPHH
-----
---LC
LMST
NCM
LDPI
IYCF
LTKK
Goat
-FPI
FYSI
VFVL
GVIA
NSY
VLW
-----
-MVN
LTM
ADLL
FLVT
LPLW
IVY
NLA
GCFF
FIN
TYCS
VAFL
AV---
I---
--FLL
SLIIW
VSIV
GAAS
YFFV
L--IH
IFLV
FSFF
LVFL
IILFC
NL-
-RA
LWM
VCTV
LAVF
VICF
VPHH
-----
-VTL
CLLS
TNCV
LDPI
IYCF
LT--
Guin
ea p
ig-F
PIVY
SIIF
VLGI
IAN
GYVL
W--
---FM
VNLT
VADL
LFLI
TLPL
WIV
-N
LAGC
LFFI
NTY
CSVA
FLGV
---I--
---I
ALSL
VIW
VAIV
AAAS
YFLV
M-
-IHIC
IVLG
FFIV
FLLI
LFCN
L--
RALW
MVC
TVLA
VFVI
CFVP
HH---
--Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Hedg
ehog
-FPI
FYSI
IFAL
GVIA
NSY
VLW
-----
FMVN
LTLA
DLLF
LVTL
PLW
II-N
LAGC
FFFI
NTY
CSVA
FLGV
---I--
--G
IILSL
VIW
VAIV
SAAS
YFFF
---IH
IFLV
VSFF
VVFL
IIFFC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
QIT
LCLL
STN
CVLD
PIIY
CFL-
--Ho
rse
--PIV
YSIIF
VLGV
IAN
SYVL
WV-
--IFM
VNLT
VADL
LFLL
TLPL
WV-
-N
LTGC
CFFI
NTY
CSVA
FLGV
---I--
---I
SLSL
VIW
LAIV
AAAS
YFLF
M-
-IHIF
LVFS
FFLV
FLIIL
VCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
-Q
VTLC
LLST
NCV
LDPI
IYCF
L---
Med
aka
-FSV
VYSI
FFVV
GLLA
NVY
VLF-
----
YMTN
LTIA
DLLF
VCAL
PFW
IG-
--TGS
FFFI
NTY
GTIL
FLGA
---IS
L-RG
IIVSV
LIW
LCLV
SSAI
PSL-
----H
FLIIG
LFFV
VFFL
VMVC
NLL
----
QM
LLAV
VGVF
LLCF
LPHH
VVQ
--Q
ITLV
LMGL
NCI
LDPV
IYYF
A---
Mon
key
-FPI
VYSI
IFVL
GVIA
NGY
VLW
-----
FMVN
LTM
ADM
LFLI
TLPL
WIV
-N
LAGC
LFFI
NTY
CSVA
FLGV
---I--
---I
SLSL
VIW
VAIV
GAAS
YFFI
L--IH
IFIV
FSFF
LVFL
IILFC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
QVT
LCLL
STN
CVLD
PVIY
CFL-
--M
ouse
-FPI
VYSV
IFIL
GVVA
NGY
VLW
-----
FMVN
LTM
ADLL
FLIT
LPLW
IV-
NVA
GCLF
FIN
TYCS
VAFL
GV---
I---
-GIS
LSLI
IWVS
IVAT
ASYF
LA--
VVHV
FIAF
CFFL
VFFL
IFYC
N---
RALW
MVC
TVLA
VFIIC
FVPH
H----
--IT
LCLL
STN
CVLD
PVIY
CFLT
--O
poss
um-F
PIVY
SIIF
VLGF
VSN
AYVL
W--
----M
VNLT
VADL
LFLF
ALPL
WIV
YN
VAGC
LFFI
NTY
CSVC
FLGV
---I--
--G
ILLS
TIIW
VVIV
GCAS
YFLI
----H
VFIV
LCFF
IVFL
VILV
CNVI
---L
WM
VCTV
MAV
FFIC
FVPH
HVV-
--Q
VTLC
LMSI
NCV
LDPI
IYCF
L---
Ora
ngut
an-F
PIVY
SIIF
VLGV
IAN
GYVL
W--
---FM
VNLT
MAD
MLF
LITL
PLW
IV-
NVA
GCLF
FIN
TYCS
VAFL
GV---
I---
--ISL
SLVI
WVA
IVGA
ASYF
LIL-
-IHIF
IVFS
FFLV
FLIIL
FCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
--V
TLCL
LSTN
CVLD
PVIY
CFLT
--Pi
g-F
PIFY
SIIF
VLGV
IAN
SYVL
W--
----M
LNLT
MAD
LLFL
VTLP
LWIIY
NLA
GCFF
FIN
TYCS
VSFL
AV---
I---
--ISL
SLIIW
VAM
VAAA
SYFF
VL-
-IHIF
LVFS
FFLV
FLVI
LFCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
--IT
LCLL
STN
CVLD
PIIY
CFLT
--Pi
ka-F
PIVY
SIIF
VLGV
VAN
GYVL
W--
---FM
VNLT
VADL
LFLI
TLPL
WII-
NVA
GCLF
FIN
TYCS
VAFL
GV---
I---
--ISL
SLIIW
VVIL
AIAS
YFLI
M-
-IHIF
IVFC
FFVV
FLIIL
VCN
L--
RALW
MVC
TVLG
VFIIC
FVPH
H----
-Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Plat
ypus
---VA
YSIIF
VLGI
FAN
GYVL
WVF
---FM
VNLT
VADL
LFLV
TMPL
WIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
---I--
--G
IVLS
LVIW
VLIV
SSAL
YFLV
---IH
IFIV
ASFF
AVFL
LILV
CNL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
-VTL
CLLS
TNCV
LDPV
IYCF
LT--
Puffe
rfish
--PSA
YGVI
FILG
LLAN
LYVL
FI-
----M
TNLS
IADL
LFVC
ALPF
WIG
Y---
GSLF
FIN
TYCS
VLFL
GA---
ISVN
-GIII
SVIIW
VLTI
SMAI
PYLV
-----
FAIIG
LFFV
VFAL
VLVC
NLL
I---
--LLA
VVGV
FVVC
FLPH
HVIQ
GAQ
ITLL
LMGL
NCI
LDPV
VYCF
A---
Rabb
it--P
IVYS
IIFVL
GVVA
NGY
VLW
V----
FMVN
LTVA
DLLF
LITL
PLW
II-N
LAGC
LFFI
NTY
CSVA
FLGV
---I--
---I
ALSL
VIW
VSIS
AAAT
YFLI
L--IH
IFIV
ASFF
IVFL
IILIC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
QVT
LCLL
STN
CVLD
PVIY
CFL-
--Ra
t--P
IVYS
VIFV
LGVV
ANGY
VLW
V----
FMVN
LTVA
DLLF
LMTL
PLW
IV-
NLA
GCLF
FIN
TYCS
VAFL
GV---
I---
-GIT
LSLV
IWIS
IAAT
ASYF
LA--
-VHI
FITS
CFFL
VFFL
IFYC
NM
--RA
LWM
VCTV
LAVF
VICF
VPHH
-----
-ITLC
LLST
NCV
LDPV
IYCF
LT--
Shre
w-F
PIFY
SIIF
VLGV
IAN
SYVL
W--
---FM
VNLT
MAD
LLFL
ITLP
LWIV
-N
LTGC
FFFI
NTY
CSVA
FLGV
---I--
--G
IYLS
LVIW
MAI
VSAA
SYFF
F--
-IHIF
LVCS
FFLV
FLLI
FFCN
L--
RALW
MVC
TVLA
VFSI
CFVP
HH---
---V
TLCL
LSTN
CVLD
PIIY
CFLT
--Sq
uirr
elLF
PIIY
SIIF
VVGV
IAN
GYVL
------
FMLN
LTVA
DLLF
LITL
PLW
II-N
VAGC
LFFI
NTY
CSVA
FLGV
---I--
----
YLSL
VIW
VFIV
AAAS
YFLI
LDVI
HIFI
VFSF
FLVF
LIIL
FCK-
--RA
LWM
VCAV
LAVF
VICF
VPHH
-----
QVT
LCLL
STN
CVLD
PVIY
CFL-
--Sti
ckleba
ck-F
PVVY
SIIF
ILGI
TAN
LYVL
F--
-CIY
MIN
LTIG
DLLF
VTFL
PFW
-----S
GSLF
FIN
TYSS
VLFL
CA---
ISV-
-GIIL
SVFI
WLF
TLAT
AILF
LI--
--HLT
IVIG
FFLV
FFVV
VVCN
FL-
---Q
MLF
SVVG
VFVV
CFLP
HHAC
Q--
QIT
WLL
MSL
NCI
LDPV
VYFF
A---
Zebrafi
sh--P
IFYG
VVFV
LGLI
ANCY
ALYV
---I
YMTN
LTVA
DLLF
VSAL
PFW
I-----
GTFF
FIN
TYGS
ILFL
TV---
ISIN
RGAI
ISVL
IWAF
ILSA
SIPQ
L---
ITHF
SIIG
LFFL
VFLM
VIIC
N---
--LRM
LCAV
IGVF
VICF
LPHH
VV---
QIT
LMLM
GIN
CLLD
PLVY
CFA-
--
5Supplementary data Search for PAF receptor in T. cruzi • Daniel Fábio Kawano et al.
Sequ
ence
Dom
ain
ID
omai
n II
Dom
ain
III
Dom
ain
IVD
omai
n V
Dom
ain
VI
Dom
ain
VII
Tc00
.104
7053
5051
93.7
4-K
DIM
FLL-
FSIG
MVF
GPHI
GYV-
---EG
YSPL
VSLI
LLAS
NGI
RILY
--ALA
LLFQ
AMF-
AILV
HTVL
LIM
------
-VLW
TLLT
TIVV
LLYY
ATIG
TVEV
VGYI
ALGI
EALL
VLPQ
ILR-
--TI
MLV
LTW
FLGD
IIKVI
YFLV
------
-PF
ILCG
I-FQ
LSLD
LVVI
GQLI
--Hu
man
-FPI
VYSI
IFVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLVI
WVA
IVGA
ASYF
LIL-
----
-IHIF
IVFS
FFLV
FLIIL
FCN
L--
RALW
MVC
TVLA
--VFI
ICFV
PHH-
----
-VTL
CLLS
TNCV
LDPV
IYCF
LT--
Chic
ken
--TVF
YSIIF
ILGF
VAN
CYVL
WI-
--FM
LNLT
VADL
LFLV
TMPL
WIV
-SV
AGSL
FFVN
TYAS
VAFL
MVI
----G
IFLS
AAIW
IITVG
SALY
YIF-
-----
AIHI
IICTL
FYVI
FLFI
LTW
N---
RALW
MVC
TVLV
--VFI
ISFV
PHH-
----
-VTL
CLLS
MN
CVLD
PVIY
CFLT
--Ch
impa
nzee
-FPI
VYSI
IFVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLVI
WVA
IVGA
ASYF
LIL-
----
-IHIF
IVFS
FFLV
FLIIL
FCN
L--
RALW
MVC
TVLA
--VFI
ICFV
PHH-
----
-VTL
CLLS
TNCV
LDPV
IYCF
LT--
Cow
-FPI
FYSI
VFVL
GVIA
NSY
VLW
-----
MVN
LTM
ADLL
FLVT
LPLW
IVY
NLA
GCFF
FIN
TYCS
VAFL
AVI--
---I
LLSL
IIWVS
IVGA
ASYF
FVL-
----
-IHIF
LVFS
FFLV
FLIIL
FCN
L--
RALW
MVC
TVLA
--VFI
ICFV
PHH-
----
-VTL
CLLS
TNCV
LDPI
IYCF
LT--
Elep
hant
-FPI
FYSI
IFVL
GVIA
NGY
VLW
-----
MVN
LTM
ADLL
FLVT
LPLW
IVY
NLA
GCLF
FIN
TYCS
VAFL
GVI--
---I
TLSL
VIW
VTIV
AAAS
YFLF
M---
----H
IFIV
CSFF
LVFF
IILVC
NLV
-RA
LWM
VCTV
LA--V
FIIC
FVPH
H----
-Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Frog
---VT
YSIIF
VFGF
IAN
CYVL
WIF
-IFM
INLT
IADL
LFLV
TLPL
WI--
NVA
GCFF
FLN
TYCS
VAFL
GVI--
----
CISV
AVW
ILLF
ASSL
YFLI
FP---
---H
FILV
GAFF
VVFL
IILTC
NLV
-RA
LWM
VVTV
LA--V
FVLC
FVPH
H----
----
LCLM
STN
CMLD
PIIY
CFLT
KKGo
at-F
PIFY
SIVF
VLGV
IAN
SYVL
W--
---M
VNLT
MAD
LLFL
VTLP
LWIV
YN
LAGC
FFFI
NTY
CSVA
FLAV
I---
--FLL
SLIIW
VSIV
GAAS
YFFV
L----
--IH
IFLV
FSFF
LVFL
IILFC
NL-
-RA
LWM
VCTV
LA--V
FVIC
FVPH
H----
--V
TLCL
LSTN
CVLD
PIIY
CFLT
--Gu
inea
pig
-FPI
VYSI
IFVL
GIIA
NGY
VLW
----F
MVN
LTVA
DLLF
LITL
PLW
IV-
NLA
GCLF
FIN
TYCS
VAFL
GVI--
---I
ALSL
VIW
VAIV
AAAS
YFLV
M---
---IH
ICIV
LGFF
IVFL
LILF
CNL-
-RA
LWM
VCTV
LA--V
FVIC
FVPH
H----
-Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Hedg
ehog
-FPI
FYSI
IFAL
GVIA
NSY
VLW
----F
MVN
LTLA
DLLF
LVTL
PLW
II-N
LAGC
FFFI
NTY
CSVA
FLGV
I---
-GIIL
SLVI
WVA
IVSA
ASYF
FF---
----IH
IFLV
VSFF
VVFL
IIFFC
NL-
-RA
LWM
VCTV
LA--V
FIIC
FVPH
H----
-Q
ITLC
LLST
NCV
LDPI
IYCF
L---
Hors
e--P
IVYS
IIFVL
GVIA
NSY
VLW
V--IF
MVN
LTVA
DLLF
LLTL
PLW
V--
NLT
GCCF
FIN
TYCS
VAFL
GVI--
---I
SLSL
VIW
LAIV
AAAS
YFLF
M---
---IH
IFLV
FSFF
LVFL
IILVC
NL-
-RA
LWM
VCTV
LA--V
FIIC
FVPH
H----
-Q
VTLC
LLST
NCV
LDPI
IYCF
L---
Med
aka
-FSV
VYSI
FFVV
GLLA
NVY
VLF-
---Y
MTN
LTIA
DLLF
VCAL
PFW
IG-
--TGS
FFFI
NTY
GTIL
FLGA
ISL-
RGIIV
SVLI
WLC
LVSS
AIPS
L----
-----H
FLIIG
LFFV
VFFL
VMVC
NLL
----
QM
LLAV
VG--V
FLLC
FLPH
HVVQ
--Q
ITLV
LMGL
NCI
LDPV
IYYF
A---
Mon
key
-FPI
VYSI
IFVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
-N
LAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLVI
WVA
IVGA
ASYF
FIL-
----
-IHIF
IVFS
FFLV
FLIIL
FCN
L--
RALW
MVC
TVLA
--VFI
ICFV
PHH-
----
QVT
LCLL
STN
CVLD
PVIY
CFL-
--M
ouse
-FPI
VYSV
IFIL
GVVA
NGY
VLW
--N
VAGC
LFFI
NTY
CSVA
FLGV
I---
--FM
VNLT
MAD
LLFL
ITLP
LWIV
--G
ISLS
LIIW
VSIV
ATAS
YFLA
------
VVHV
FIAF
CFFL
VFFL
IFYC
N---
RALW
MVC
TVLA
--VFI
ICFV
PHH-
----
-ITLC
LLST
NCV
LDPV
IYCF
LT--
Opo
ssum
-FPI
VYSI
IFVL
GFVS
NAY
VLW
-----
MVN
LTVA
DLLF
LFAL
PLW
IVY
NVA
GCLF
FIN
TYCS
VCFL
GVI--
--G
ILLS
TIIW
VVIV
GCAS
YFLI
------
--HVF
IVLC
FFIV
FLVI
LVCN
VI-
--LW
MVC
TVM
A--V
FFIC
FVPH
HVV-
--Q
VTLC
LMSI
NCV
LDPI
IYCF
L---
Ora
ngut
an-F
PIVY
SIIF
VLGV
IAN
GYVL
W--
--FM
VNLT
MAD
MLF
LITL
PLW
IV-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
---I
SLSL
VIW
VAIV
GAAS
YFLI
L----
--IH
IFIV
FSFF
LVFL
IILFC
NL-
-RA
LWM
VCTV
LA--V
FIIC
FVPH
H----
--V
TLCL
LSTN
CVLD
PVIY
CFLT
--Pi
g-F
PIFY
SIIF
VLGV
IAN
SYVL
W--
---M
LNLT
MAD
LLFL
VTLP
LWIIY
NLA
GCFF
FIN
TYCS
VSFL
AVI--
--IH
IFLV
FSFF
LVFL
VILF
CNL-
---I
SLSL
IIWVA
MVA
AASY
FFVL
-----
RALW
MVC
TVLA
--VFI
ICFV
PHH-
----
-ITLC
LLST
NCV
LDPI
IYCF
LT--
Pika
-FPI
VYSI
IFVL
GVVA
NGY
VLW
----F
MVN
LTVA
DLLF
LITL
PLW
II-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLIIW
VVIL
AIAS
YFLI
M---
---IH
IFIV
FCFF
VVFL
IILVC
NL-
-RA
LWM
VCTV
LG--V
FIIC
FVPH
H----
-Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Plat
ypus
---VA
YSIIF
VLGI
FAN
GYVL
WVF
--FM
VNLT
VADL
LFLV
TMPL
WIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
-GIV
LSLV
IWVL
IVSS
ALYF
LV---
----IH
IFIV
ASFF
AVFL
LILV
CNL-
-RA
LWM
VCTV
LA--V
FIIC
FVPH
H----
--V
TLCL
LSTN
CVLD
PVIY
CFLT
--Pu
fferfish
--PSA
YGVI
FILG
LLAN
LYVL
FI-
---M
TNLS
IADL
LFVC
ALPF
WIG
Y---
GSLF
FIN
TYCS
VLFL
GAIS
VN-G
IIISV
IIWVL
TISM
AIPY
LV---
------
FAIIG
LFFV
VFAL
VLVC
NLL
I---
--LLA
VVG-
-VFV
VCFL
PHHV
IQGA
QIT
LLLM
GLN
CILD
PVVY
CFA-
--Ra
bbit
--PIV
YSIIF
VLGV
VAN
GYVL
WV-
--FM
VNLT
VADL
LFLI
TLPL
WII-
NLA
GCLF
FIN
TYCS
VAFL
GVI--
---I
ALSL
VIW
VSIS
AAAT
YFLI
L----
--IH
IFIV
ASFF
IVFL
IILIC
NL-
-RA
LWM
VCTV
LA--V
FIIC
FVPH
H----
-Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Rat
--PIV
YSVI
FVLG
VVAN
GYVL
WV-
--FM
VNLT
VADL
LFLM
TLPL
WIV
-N
LAGC
LFFI
NTY
CSVA
FLGV
I---
-GIT
LSLV
IWIS
IAAT
ASYF
LA---
---RA
LWM
VCTV
LA--V
FVIC
FVPH
H----
--V
HIFI
TSCF
FLVF
FLIF
YCN
M--
-ITLC
LLST
NCV
LDPV
IYCF
LT--
Shre
w-F
PIFY
SIIF
VLGV
IAN
SYVL
W--
--FM
VNLT
MAD
LLFL
ITLP
LWIV
--G
IYLS
LVIW
MAI
VSAA
SYFF
F----
--N
LTGC
FFFI
NTY
CSVA
FLGV
I---
-IHIF
LVCS
FFLV
FLLI
FFCN
L--
RALW
MVC
TVLA
--VFS
ICFV
PHH-
----
-VTL
CLLS
TNCV
LDPI
IYCF
LT--
Squi
rrel
LFPI
IYSI
IFVV
GVIA
NGY
VL---
--FM
LNLT
VADL
LFLI
TLPL
WII-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
----
YLSL
VIW
VFIV
AAAS
YFLI
LD---
-VI
HIFI
VFSF
FLVF
LIIL
FCK-
--RA
LWM
VCAV
LA--V
FVIC
FVPH
H----
-Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Stickleba
ck-F
PVVY
SIIF
ILGI
TAN
LYVL
F--
CIYM
INLT
IGDL
LFVT
FLPF
W---
--SGS
LFFI
NTY
SSVL
FLCA
ISV-
-GIIL
SVFI
WLF
TLAT
AILF
LI---
-----H
LTIV
IGFF
LVFF
VVVV
CNFL
----
QM
LFSV
VG--V
FVVC
FLPH
HACQ
--Q
ITW
LLM
SLN
CILD
PVVY
FFA-
--Zebrafi
sh--P
IFYG
VVFV
LGLI
ANCY
ALYV
--IY
MTN
LTVA
DLLF
VSAL
PFW
I-----
GTFF
FIN
TYGS
ILFL
TVIS
INRG
AIIS
VLIW
AFIL
SASI
PQL-
------
ITHF
SIIG
LFFL
VFLM
VIIC
N---
--LRM
LCAV
IG--V
FVIC
FLPH
HVV-
--Q
ITLM
LMGI
NCL
LDPL
VYCF
A---
Sequ
ence
Dom
ain
ID
omai
n II
Dom
ain
III
Dom
ain
IVD
omai
n V
Dom
ain
VI
Dom
ain
VII
Tc00
.104
7053
5034
63.2
0-L
STFK
GIIG
AYYG
LPFS
GTGS
-----
LPIM
ISCT
LLPL
VFLG
FLLR
I---
----C
LGFL
SDAF
VLM
FGIT
ALAS
Q---
FLGF
SILI
SCTR
LVSI
LILF
PSV
AVFP
IMFG
YAFC
GHVV
FGA-
------
--SRS
IVTL
FCTT
FGDN
IIDTF
L-F
GRLF
IGTY
LLL-
-FIC
NIL
NVA
Hum
an-F
PIVY
SII--
-FVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
-N
VAGC
LFFI
NTY
CSVA
F-LG
VI---
--ISL
SLVI
WVA
IVGA
ASYF
LIL-
-IHIF
-IVFS
FFLV
FLIIL
FCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
--V
TLCL
LSTN
CVLD
PVIY
CFLT
--Ch
icke
n--T
VFYS
II---F
ILGF
VAN
CYVL
WI-
--FM
LNLT
VADL
LFLV
TMPL
WIV
-SV
AGSL
FFVN
TYAS
VAF-
LMVI
----G
IFLS
AAIW
IITVG
SALY
YIF-
-AI
HII-I
CTLF
YVIF
LFILT
WN
---RA
LWM
VCTV
LVVF
IISFV
PHH-
----
-VTL
CLLS
MN
CVLD
PVIY
CFLT
--Ch
impa
nzee
-FPI
VYSI
I---F
VLGV
IAN
GYVL
W--
--FM
VNLT
MAD
MLF
LITL
PLW
IV-
NVA
GCLF
FIN
TYCS
VAF-
LGVI
-----I
SLSL
VIW
VAIV
GAAS
YFLI
L--IH
IF-IV
FSFF
LVFL
IILFC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
-VTL
CLLS
TNCV
LDPV
IYCF
LT--
Cow
-FPI
FYSI
V---F
VLGV
IAN
SYVL
W--
---M
VNLT
MAD
LLFL
VTLP
LWIV
YN
LAGC
FFFI
NTY
CSVA
F-LA
VI---
--ILL
SLIIW
VSIV
GAAS
YFFV
L--IH
IF-L
VFSF
FLVF
LIIL
FCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
--V
TLCL
LSTN
CVLD
PIIY
CFLT
--El
epha
nt-F
PIFY
SII--
-FVL
GVIA
NGY
VLW
-----
MVN
LTM
ADLL
FLVT
LPLW
IVY
NLA
GCLF
FIN
TYCS
VAF-
LGVI
-----I
TLSL
VIW
VTIV
AAAS
YFLF
M-
--HIF
-IVCS
FFLV
FFIIL
VCN
LV-
RALW
MVC
TVLA
VFIIC
FVPH
H----
-Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Frog
---VT
YSII-
--FVF
GFIA
NCY
VLW
IF-IF
MIN
LTIA
DLLF
LVTL
PLW
I--N
VAGC
FFFL
NTY
CSVA
F-LG
VI---
--HFI
-LVG
AFFV
VFLI
ILTCN
LV-
---CI
SVAV
WIL
LFAS
SLYF
LIFP
RALW
MVV
TVLA
VFVL
CFVP
HH---
-----
LCLM
STN
CMLD
PIIY
CFLT
KKGo
at-F
PIFY
SIV-
--FVL
GVIA
NSY
VLW
-----
MVN
LTM
ADLL
FLVT
LPLW
IVY
NLA
GCFF
FIN
TYCS
VAF-
LAVI
-----F
LLSL
IIWVS
IVGA
ASYF
FVL-
-IHIF
-LVF
SFFL
VFLI
ILFC
NL-
-RA
LWM
VCTV
LAVF
VICF
VPHH
-----
-VTL
CLLS
TNCV
LDPI
IYCF
LT--
Guin
ea p
ig-F
PIVY
SII--
-FVL
GIIA
NGY
VLW
----F
MVN
LTVA
DLLF
LITL
PLW
IV-
NLA
GCLF
FIN
TYCS
VAF-
LGVI
-----I
ALSL
VIW
VAIV
AAAS
YFLV
M-
-IHIC
-IVLG
FFIV
FLLI
LFCN
L--
RALW
MVC
TVLA
VFVI
CFVP
HH---
--Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Hedg
ehog
-FPI
FYSI
I---F
ALGV
IAN
SYVL
W--
--FM
VNLT
LADL
LFLV
TLPL
WII-
NLA
GCFF
FIN
TYCS
VAF-
LGVI
----G
IILSL
VIW
VAIV
SAAS
YFFF
---IH
IF-L
VVSF
FVVF
LIIF
FCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
-Q
ITLC
LLST
NCV
LDPI
IYCF
L---
Hors
e--P
IVYS
II---F
VLGV
IAN
SYVL
WV-
-IFM
VNLT
VADL
LFLL
TLPL
WV-
-N
LTGC
CFFI
NTY
CSVA
F-LG
VI---
--ISL
SLVI
WLA
IVAA
ASYF
LFM
--IH
IF-L
VFSF
FLVF
LIILV
CNL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
QVT
LCLL
STN
CVLD
PIIY
CFL-
--M
edak
a-F
SVVY
SIF-
--FVV
GLLA
NVY
VLF-
---Y
MTN
LTIA
DLLF
VCAL
PFW
IG-
RGIIV
SVLI
WLC
LVSS
AIPS
L---
--TGS
FFFI
NTY
GTIL
F-LG
AISL
----
HFLI
IGLF
FVVF
FLVM
VCN
LL-
---Q
MLL
AVVG
VFLL
CFLP
HHVV
Q--
QIT
LVLM
GLN
CILD
PVIY
YFA-
--M
onke
y-F
PIVY
SII--
-FVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
-N
LAGC
LFFI
NTY
CSVA
F-LG
VI---
--ISL
SLVI
WVA
IVGA
ASYF
FIL-
-IHIF
-IVFS
FFLV
FLIIL
FCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
-Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Mou
se-F
PIVY
SVI--
-FIL
GVVA
NGY
VLW
----F
MVN
LTM
ADLL
FLIT
LPLW
IV-
NVA
GCLF
FIN
TYCS
VAF-
LGVI
----G
ISLS
LIIW
VSIV
ATAS
YFLA
--VV
HVF-
IAFC
FFLV
FFLI
FYCN
---RA
LWM
VCTV
LAVF
IICFV
PHH-
----
-ITLC
LLST
NCV
LDPV
IYCF
LT--
Opo
ssum
-FPI
VYSI
I---F
VLGF
VSN
AYVL
W--
NVA
GCLF
FIN
TYCS
VCF-
LGVI
------
MVN
LTVA
DLLF
LFAL
PLW
IVY
-GIL
LSTI
IWVV
IVGC
ASYF
LI--
--HVF
-IVLC
FFIV
FLVI
LVCN
VI-
--LW
MVC
TVM
AVFF
ICFV
PHHV
V---
QVT
LCLM
SIN
CVLD
PIIY
CFL-
--O
rang
utan
-FPI
VYSI
I---F
VLGV
IAN
GYVL
W--
--FM
VNLT
MAD
MLF
LITL
PLW
IV-
--ISL
SLVI
WVA
IVGA
ASYF
LIL-
NVA
GCLF
FIN
TYCS
VAF-
LGVI
----IH
IF-IV
FSFF
LVFL
IILFC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
-VTL
CLLS
TNCV
LDPV
IYCF
LT--
Pig
-FPI
FYSI
I---F
VLGV
IAN
SYVL
W--
---M
LNLT
MAD
LLFL
VTLP
LWIIY
NLA
GCFF
FIN
TYCS
VSF-
LAVI
-----I
SLSL
IIWVA
MVA
AASY
FFVL
--IH
IF-L
VFSF
FLVF
LVIL
FCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
--IT
LCLL
STN
CVLD
PIIY
CFLT
--Pi
ka-F
PIVY
SII--
-FVL
GVVA
NGY
VLW
----F
MVN
LTVA
DLLF
LITL
PLW
II-N
VAGC
LFFI
NTY
CSVA
F-LG
VI---
--ISL
SLIIW
VVIL
AIAS
YFLI
M-
-IHIF
-IVFC
FFVV
FLIIL
VCN
L--
RALW
MVC
TVLG
VFIIC
FVPH
H----
-Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Plat
ypus
---VA
YSII-
--FVL
GIFA
NGY
VLW
VF--F
MVN
LTVA
DLLF
LVTM
PLW
IV-
NVA
GCLF
FIN
TYCS
VAF-
LGVI
----G
IVLS
LVIW
VLIV
SSAL
YFLV
---IH
IF-IV
ASFF
AVFL
LILV
CNL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
-VTL
CLLS
TNCV
LDPV
IYCF
LT--
Puffe
rfish
--PSA
YGVI
---FI
LGLL
ANLY
VLFI
----
MTN
LSIA
DLLF
VCAL
PFW
IGY
---GS
LFFI
NTY
CSVL
F-LG
AISV
N-G
IIISV
IIWVL
TISM
AIPY
LV--
----F
AIIG
LFFV
VFAL
VLVC
NLL
I---
--LLA
VVGV
FVVC
FLPH
HVIQ
GAQ
ITLL
LMGL
NCI
LDPV
VYCF
A---
Rabb
it--P
IVYS
II---F
VLGV
VAN
GYVL
WV-
--FM
VNLT
VADL
LFLI
TLPL
WII-
NLA
GCLF
FIN
TYCS
VAF-
LGVI
-----I
ALSL
VIW
VSIS
AAAT
YFLI
L--IH
IF-IV
ASFF
IVFL
IILIC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
QVT
LCLL
STN
CVLD
PVIY
CFL-
--Ra
t--P
IVYS
VI---
FVLG
VVAN
GYVL
WV-
--FM
VNLT
VADL
LFLM
TLPL
WIV
-N
LAGC
LFFI
NTY
CSVA
F-LG
VI---
-GIT
LSLV
IWIS
IAAT
ASYF
LA--
-VHI
F-IT
SCFF
LVFF
LIFY
CNM
--RA
LWM
VCTV
LAVF
VICF
VPHH
-----
-ITLC
LLST
NCV
LDPV
IYCF
LT--
Shre
w-F
PIFY
SII--
-FVL
GVIA
NSY
VLW
----F
MVN
LTM
ADLL
FLIT
LPLW
IV-
NLT
GCFF
FIN
TYCS
VAF-
LGVI
----G
IYLS
LVIW
MAI
VSAA
SYFF
F--
-IHIF
-LVC
SFFL
VFLL
IFFC
NL-
--V
TLCL
LSTN
CVLD
PIIY
CFLT
--RA
LWM
VCTV
LAVF
SICF
VPHH
-----
Squi
rrel
LFPI
IYSI
I---F
VVGV
IAN
GYVL
-----F
MLN
LTVA
DLLF
LITL
PLW
II-N
VAGC
LFFI
NTY
CSVA
F-LG
VI---
---YL
SLVI
WVF
IVAA
ASYF
LILD
VIHI
F-IV
FSFF
LVFL
IILFC
K---
RALW
MVC
AVLA
VFVI
CFVP
HH---
--Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Stickleba
ck-F
PVVY
SII--
-FIL
GITA
NLY
VLF-
-CI
YMIN
LTIG
DLLF
VTFL
PFW
-----S
GSLF
FIN
TYSS
VLF-
LCAI
SV-
-GIIL
SVFI
WLF
TLAT
AILF
LI--
--HLT
-IVIG
FFLV
FFVV
VVCN
FL-
---Q
MLF
SVVG
VFVV
CFLP
HHAC
Q--
QIT
WLL
MSL
NCI
LDPV
VYFF
A---
Zebrafi
sh--P
IFYG
VV---
FVLG
LIAN
CYAL
YV-
-IYM
TNLT
VADL
LFVS
ALPF
WI--
---GT
FFFI
NTY
GSIL
F-LT
VISI
NRG
AIIS
VLIW
AFIL
SASI
PQL-
---IT
HFSI
IGLF
FLVF
LMVI
ICN
-----L
RMLC
AVIG
VFVI
CFLP
HHVV
---Q
ITLM
LMGI
NCL
LDPL
VYCF
A---
6Supplementary data Search for PAF receptor in T. cruzi • Daniel Fábio Kawano et al.
Sequ
ence
Dom
ain
ID
omai
n II
Dom
ain
III
Dom
ain
IVD
omai
n V
Dom
ain
VI
Dom
ain
VII
Tc00
.104
7053
5041
37.1
50--H
LVGF
LAFL
VVVV
CF-F
VIVL
IYF
LFAA
YSFG
CLM
CMLC
STVF
---YF
GITF
LVVG
SFLP
FCYF
SFA-
---W
TYLS
MIS
FFGV
FGVL
GPFF
K--
LFYV
CMVG
SGLF
PIVH
IYLL
L----
-VE
GLLL
MM
TLYG
VGVF
VYAF
Q---
-----
IWHV
FVLA
A----
-AFV
HFFN
TASI
YHu
man
-FPI
VYSI
IFVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLVI
WVA
IVGA
ASYF
LIL-
-IHIF
IVFS
--FFL
VFLI
ILFC
NL-
--R
ALW
MVC
TVLA
V--F
IICFV
PHH-
----
-VTL
CLLS
TNCV
LDPV
IYCF
LT---
-Ch
icke
n--T
VFYS
IIFIL
GFVA
NCY
VLW
I---F
MLN
LTVA
DLLF
LVTM
PLW
IV-
SVAG
SLFF
VNTY
ASVA
FLM
VI---
-GIF
LSAA
IWIIT
VGSA
LYYI
F--
AIHI
IICTL
--FYV
IFLF
ILTW
N---
-RAL
WM
VCTV
LVV-
-FIIS
FVPH
H----
--V
TLCL
LSM
NCV
LDPV
IYCF
LT---
-Ch
impa
nzee
-FPI
VYSI
IFVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLVI
WVA
IVGA
ASYF
LIL-
-IHIF
IVFS
--FFL
VFLI
ILFC
NL-
--R
ALW
MVC
TVLA
V--F
IICFV
PHH-
----
-VTL
CLLS
TNCV
LDPV
IYCF
LT---
-Co
w-F
PIFY
SIVF
VLGV
IAN
SYVL
W--
NLA
GCFF
FIN
TYCS
VAFL
AVI--
----
MVN
LTM
ADLL
FLVT
LPLW
IVY
--ILL
SLIIW
VSIV
GAAS
YFFV
L--IH
IFLV
FS--F
FLVF
LIIL
FCN
L--
-RAL
WM
VCTV
LAV-
-FIIC
FVPH
H----
--V
TLCL
LSTN
CVLD
PIIY
CFLT
----
Elep
hant
-FPI
FYSI
IFVL
GVIA
NGY
VLW
-----
MVN
LTM
ADLL
FLVT
LPLW
IVY
NLA
GCLF
FIN
TYCS
VAFL
GVI--
---I
TLSL
VIW
VTIV
AAAS
YFLF
M-
--HIF
IVCS
--FFL
VFFI
ILVCN
LV-
-RAL
WM
VCTV
LAV-
-FIIC
FVPH
H----
-Q
VTLC
LLST
NCV
LDPV
IYCF
L----
-Fr
og---
VTYS
IIFVF
GFIA
NCY
VLW
IF-IF
MIN
LTIA
DLLF
LVTL
PLW
I--N
VAGC
FFFL
NTY
CSVA
FLGV
I---
---CI
SVAV
WIL
LFAS
SLYF
LIFP
--HFI
LVGA
--FFV
VFLI
ILTCN
LV-
-RAL
WM
VVTV
LAV-
-FVL
CFVP
HH---
-----
LCLM
STN
CMLD
PIIY
CFLT
KK--
Goat
-FPI
FYSI
VFVL
GVIA
NSY
VLW
-----
MVN
LTM
ADLL
FLVT
LPLW
IVY
NLA
GCFF
FIN
TYCS
VAFL
AVI--
---F
LLSL
IIWVS
IVGA
ASYF
FVL-
-IHIF
LVFS
--FFL
VFLI
ILFC
NL-
--R
ALW
MVC
TVLA
V--F
VICF
VPHH
-----
-VTL
CLLS
TNCV
LDPI
IYCF
LT---
-Gu
inea
pig
-FPI
VYSI
IFVL
GIIA
NGY
VLW
----F
MVN
LTVA
DLLF
LITL
PLW
IV-
NLA
GCLF
FIN
TYCS
VAFL
GVI--
---I
ALSL
VIW
VAIV
AAAS
YFLV
M-
-IHIC
IVLG
--FFI
VFLL
ILFC
NL-
--R
ALW
MVC
TVLA
V--F
VICF
VPHH
-----
QVT
LCLL
STN
CVLD
PVIY
CFL-
----
Hedg
ehog
-FPI
FYSI
IFAL
GVIA
NSY
VLW
----F
MVN
LTLA
DLLF
LVTL
PLW
II-N
LAGC
FFFI
NTY
CSVA
FLGV
I---
-GIIL
SLVI
WVA
IVSA
ASYF
FF--
-IHIF
LVVS
--FFV
VFLI
IFFC
NL-
--R
ALW
MVC
TVLA
V--F
IICFV
PHH-
----
QIT
LCLL
STN
CVLD
PIIY
CFL-
----
Hors
e--P
IVYS
IIFVL
GVIA
NSY
VLW
V--IF
MVN
LTVA
DLLF
LLTL
PLW
V--
NLT
GCCF
FIN
TYCS
VAFL
GVI--
---I
SLSL
VIW
LAIV
AAAS
YFLF
M-
-IHIF
LVFS
--FFL
VFLI
ILVCN
L--
-RAL
WM
VCTV
LAV-
-FIIC
FVPH
H----
-Q
VTLC
LLST
NCV
LDPI
IYCF
L----
-M
edak
a-F
SVVY
SIFF
VVGL
LAN
VYVL
F--
--YM
TNLT
IADL
LFVC
ALPF
WIG
-RG
IIVSV
LIW
LCLV
SSAI
PSL-
----T
GSFF
FIN
TYGT
ILFL
GAIS
L---H
FLIIG
L--F
FVVF
FLVM
VCN
LL-
----Q
MLL
AVVG
V--F
LLCF
LPHH
VVQ
--Q
ITLV
LMGL
NCI
LDPV
IYYF
A----
-M
onke
y-F
PIVY
SIIF
VLGV
IAN
GYVL
W--
--FM
VNLT
MAD
MLF
LITL
PLW
IV-
NLA
GCLF
FIN
TYCS
VAFL
GVI--
---I
SLSL
VIW
VAIV
GAAS
YFFI
L--IH
IFIV
FS--F
FLVF
LIIL
FCN
L--
-RAL
WM
VCTV
LAV-
-FIIC
FVPH
H----
-Q
VTLC
LLST
NCV
LDPV
IYCF
L----
-M
ouse
-FPI
VYSV
IFIL
GVVA
NGY
VLW
----F
MVN
LTM
ADLL
FLIT
LPLW
IV-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
--G
ISLS
LIIW
VSIV
ATAS
YFLA
--VV
HVFI
AFC-
-FFL
VFFL
IFYC
N---
-RAL
WM
VCTV
LAV-
-FIIC
FVPH
H----
--IT
LCLL
STN
CVLD
PVIY
CFLT
----
Opo
ssum
-FPI
VYSI
IFVL
GFVS
NAY
VLW
--Q
VTLC
LMSI
NCV
LDPI
IYCF
L----
----
MVN
LTVA
DLLF
LFAL
PLW
IVY
NVA
GCLF
FIN
TYCS
VCFL
GVI--
--G
ILLS
TIIW
VVIV
GCAS
YFLI
----H
VFIV
LC--F
FIVF
LVILV
CNVI
----
LWM
VCTV
MAV
--FFI
CFVP
HHVV
---O
rang
utan
-FPI
VYSI
IFVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLVI
WVA
IVGA
ASYF
LIL-
-IHIF
IVFS
--FFL
VFLI
ILFC
NL-
--R
ALW
MVC
TVLA
V--F
IICFV
PHH-
----
-VTL
CLLS
TNCV
LDPV
IYCF
LT---
-Pi
g-F
PIFY
SIIF
VLGV
IAN
SYVL
W--
---M
LNLT
MAD
LLFL
VTLP
LWIIY
NLA
GCFF
FIN
TYCS
VSFL
AVI--
---I
SLSL
IIWVA
MVA
AASY
FFVL
--IH
IFLV
FS--F
FLVF
LVIL
FCN
L--
-RAL
WM
VCTV
LAV-
-FIIC
FVPH
H----
--IT
LCLL
STN
CVLD
PIIY
CFLT
----
Pika
-FPI
VYSI
IFVL
GVVA
NGY
VLW
----F
MVN
LTVA
DLLF
LITL
PLW
II-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLIIW
VVIL
AIAS
YFLI
M-
-IHIF
IVFC
--FFV
VFLI
ILVCN
L--
-RAL
WM
VCTV
LGV-
-FIIC
FVPH
H----
-Q
VTLC
LLST
NCV
LDPV
IYCF
L----
-Pl
atyp
us---
VAYS
IIFVL
GIFA
NGY
VLW
VF--F
MVN
LTVA
DLLF
LVTM
PLW
IV-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
--G
IVLS
LVIW
VLIV
SSAL
YFLV
---IH
IFIV
AS--F
FAVF
LLILV
CNL-
--R
ALW
MVC
TVLA
V--F
IICFV
PHH-
----
-VTL
CLLS
TNCV
LDPV
IYCF
LT---
-Pu
fferfish
--PSA
YGVI
FILG
LLAN
LYVL
FI-
---M
TNLS
IADL
LFVC
ALPF
WIG
Y---
GSLF
FIN
TYCS
VLFL
GAIS
VN-G
IIISV
IIWVL
TISM
AIPY
LV--
---FA
IIGL-
-FFV
VFAL
VLVC
NLL
I---
---LL
AVVG
V--F
VVCF
LPHH
VIQ
GAQ
ITLL
LMGL
NCI
LDPV
VYCF
A----
-Ra
bbit
--PIV
YSIIF
VLGV
VAN
GYVL
WV-
--FM
VNLT
VADL
LFLI
TLPL
WII-
NLA
GCLF
FIN
TYCS
VAFL
GVI--
---I
ALSL
VIW
VSIS
AAAT
YFLI
L--IH
IFIV
AS--F
FIVF
LIIL
ICN
L--
-RAL
WM
VCTV
LAV-
-FIIC
FVPH
H----
-Q
VTLC
LLST
NCV
LDPV
IYCF
L----
-Ra
t--P
IVYS
VIFV
LGVV
ANGY
VLW
V---F
MVN
LTVA
DLLF
LMTL
PLW
IV-
NLA
GCLF
FIN
TYCS
VAFL
GVI--
--G
ITLS
LVIW
ISIA
ATAS
YFLA
---V
HIFI
TSC-
-FFL
VFFL
IFYC
NM
---R
ALW
MVC
TVLA
V--F
VICF
VPHH
-----
-ITLC
LLST
NCV
LDPV
IYCF
LT---
-Sh
rew
-FPI
FYSI
IFVL
GVIA
NSY
VLW
----F
MVN
LTM
ADLL
FLIT
LPLW
IV-
NLT
GCFF
FIN
TYCS
VAFL
GVI--
--G
IYLS
LVIW
MAI
VSAA
SYFF
F--
-IHIF
LVCS
--FFL
VFLL
IFFC
NL-
--R
ALW
MVC
TVLA
V--F
SICF
VPHH
-----
-VTL
CLLS
TNCV
LDPI
IYCF
LT---
-Sq
uirr
elLF
PIIY
SIIF
VVGV
IAN
GYVL
-----F
MLN
LTVA
DLLF
LITL
PLW
II-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
---YL
SLVI
WVF
IVAA
ASYF
LILD
VIHI
FIVF
S--F
FLVF
LIIL
FCK-
---R
ALW
MVC
AVLA
V--F
VICF
VPHH
-----
QVT
LCLL
STN
CVLD
PVIY
CFL-
----
Stickleba
ck-F
PVVY
SIIF
ILGI
TAN
LYVL
F--
CIYM
INLT
IGDL
LFVT
FLPF
W---
--SGS
LFFI
NTY
SSVL
FLCA
ISV-
-GIIL
SVFI
WLF
TLAT
AILF
LI--
--HLT
IVIG
--FFL
VFFV
VVVC
NFL
----
-QM
LFSV
VGV-
-FVV
CFLP
HHAC
Q--
QIT
WLL
MSL
NCI
LDPV
VYFF
A----
-Zebrafi
sh-IY
MTN
LTVA
DLLF
VSAL
PFW
I----P
IFYG
VVFV
LGLI
ANCY
ALYV
----
GTFF
FIN
TYGS
ILFL
TVIS
INRG
AIIS
VLIW
AFIL
SASI
PQL-
-----
LRM
LCAV
IGV-
-FVI
CFLP
HHVV
---IT
HFSI
IGL-
-FFL
VFLM
VIIC
N---
QIT
LMLM
GIN
CLLD
PLVY
CFA-
----
Sequ
ence
Dom
ain
ID
omai
n II
Dom
ain
III
Dom
ain
IVD
omai
n V
Dom
ain
VI
Dom
ain
VII
Tc00
.104
7053
5064
67.4
-KDI
MFL
L-FS
IGM
VFGP
HIGY
V----
EGYS
PLVS
LILL
ASN
GIRI
LY--A
LALL
FQAM
F-AI
LVHT
VLLI
M---
----V
LWTL
LTTI
LVLL
YYAT
IGTV
EVVG
YIAL
GIEA
LLVL
PQIL
R---
TIM
LVLT
WFL
GDIIK
VIYF
LV---
----
PFIF
CGI-F
QLS
LDLV
VIGQ
LI--
Hum
an-F
PIVY
SIIF
VLGV
IAN
GYVL
W--
--FM
VNLT
MAD
MLF
LITL
PLW
IV-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
---I
SLSL
VIW
VAIV
GAAS
YFLI
L----
--IH
IFIV
FSFF
LVFL
IILFC
NL-
-RA
LWM
VCTV
LA--V
FIIC
FVPH
H----
--V
TLCL
LSTN
CVLD
PVIY
CFLT
--Ch
icke
n--T
VFYS
IIFIL
GFVA
NCY
VLW
I---F
MLN
LTVA
DLLF
LVTM
PLW
IV-
SVAG
SLFF
VNTY
ASVA
FLM
VI---
-GIF
LSAA
IWIIT
VGSA
LYYI
F----
--AI
HIIIC
TLFY
VIFL
FILT
WN
---RA
LWM
VCTV
LV--V
FIIS
FVPH
H----
--V
TLCL
LSM
NCV
LDPV
IYCF
LT--
Chim
panz
ee-F
PIVY
SIIF
VLGV
IAN
GYVL
W--
--FM
VNLT
MAD
MLF
LITL
PLW
IV-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
---I
SLSL
VIW
VAIV
GAAS
YFLI
L----
--IH
IFIV
FSFF
LVFL
IILFC
NL-
-RA
LWM
VCTV
LA--V
FIIC
FVPH
H----
--V
TLCL
LSTN
CVLD
PVIY
CFLT
--Co
w-F
PIFY
SIVF
VLGV
IAN
SYVL
W--
---M
VNLT
MAD
LLFL
VTLP
LWIV
YN
LAGC
FFFI
NTY
CSVA
FLAV
I---
--ILL
SLIIW
VSIV
GAAS
YFFV
L----
--IH
IFLV
FSFF
LVFL
IILFC
NL-
-RA
LWM
VCTV
LA--V
FIIC
FVPH
H----
--V
TLCL
LSTN
CVLD
PIIY
CFLT
--El
epha
nt-F
PIFY
SIIF
VLGV
IAN
GYVL
W--
---M
VNLT
MAD
LLFL
VTLP
LWIV
YN
LAGC
LFFI
NTY
CSVA
FLGV
I---
--ITL
SLVI
WVT
IVAA
ASYF
LFM
-----
--HIF
IVCS
FFLV
FFIIL
VCN
LV-
RALW
MVC
TVLA
--VFI
ICFV
PHH-
----
QVT
LCLL
STN
CVLD
PVIY
CFL-
--Fr
og---
VTYS
IIFVF
GFIA
NCY
VLW
IF-IF
MIN
LTIA
DLLF
LVTL
PLW
I--N
VAGC
FFFL
NTY
CSVA
FLGV
I---
---CI
SVAV
WIL
LFAS
SLYF
LIFP
----
--HFI
LVGA
FFVV
FLIIL
TCN
LV-
RALW
MVV
TVLA
--VFV
LCFV
PHH-
----
---LC
LMST
NCM
LDPI
IYCF
LTKK
Goat
-FPI
FYSI
VFVL
GVIA
NSY
VLW
-----
MVN
LTM
ADLL
FLVT
LPLW
IVY
NLA
GCFF
FIN
TYCS
VAFL
AVI--
--IH
IFLV
FSFF
LVFL
IILFC
NL-
---F
LLSL
IIWVS
IVGA
ASYF
FVL-
----
RALW
MVC
TVLA
--VFV
ICFV
PHH-
----
-VTL
CLLS
TNCV
LDPI
IYCF
LT--
Guin
ea p
ig-F
PIVY
SIIF
VLGI
IAN
GYVL
W--
--FM
VNLT
VADL
LFLI
TLPL
WIV
-N
LAGC
LFFI
NTY
CSVA
FLGV
I---
--IAL
SLVI
WVA
IVAA
ASYF
LVM
-----
-IHIC
IVLG
FFIV
FLLI
LFCN
L--
RALW
MVC
TVLA
--VFV
ICFV
PHH-
----
QVT
LCLL
STN
CVLD
PVIY
CFL-
--He
dgeh
og-F
PIFY
SIIF
ALGV
IAN
SYVL
W--
--FM
VNLT
LADL
LFLV
TLPL
WII-
NLA
GCFF
FIN
TYCS
VAFL
GVI--
--G
IILSL
VIW
VAIV
SAAS
YFFF
------
-IHIF
LVVS
FFVV
FLIIF
FCN
L--
RALW
MVC
TVLA
--VFI
ICFV
PHH-
----
QIT
LCLL
STN
CVLD
PIIY
CFL-
--Ho
rse
--PIV
YSIIF
VLGV
IAN
SYVL
WV-
-IFM
VNLT
VADL
LFLL
TLPL
WV-
-N
LTGC
CFFI
NTY
CSVA
FLGV
I---
--ISL
SLVI
WLA
IVAA
ASYF
LFM
-----
-IHIF
LVFS
FFLV
FLIIL
VCN
L--
RALW
MVC
TVLA
--VFI
ICFV
PHH-
----
QVT
LCLL
STN
CVLD
PIIY
CFL-
--M
edak
a-F
SVVY
SIFF
VVGL
LAN
VYVL
F--
--YM
TNLT
IADL
LFVC
ALPF
WIG
---T
GSFF
FIN
TYGT
ILFL
GAIS
L-RG
IIVSV
LIW
LCLV
SSAI
PSL-
------
--HFL
IIGLF
FVVF
FLVM
VCN
LL-
---Q
MLL
AVVG
--VFL
LCFL
PHHV
VQ--
QIT
LVLM
GLN
CILD
PVIY
YFA-
--M
onke
y-F
PIVY
SIIF
VLGV
IAN
GYVL
W--
--FM
VNLT
MAD
MLF
LITL
PLW
IV-
NLA
GCLF
FIN
TYCS
VAFL
GVI--
---I
SLSL
VIW
VAIV
GAAS
YFFI
L----
--IH
IFIV
FSFF
LVFL
IILFC
NL-
-RA
LWM
VCTV
LA--V
FIIC
FVPH
H----
-Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Mou
se-F
PIVY
SVIF
ILGV
VAN
GYVL
W--
--FM
VNLT
MAD
LLFL
ITLP
LWIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
-GIS
LSLI
IWVS
IVAT
ASYF
LA---
---VV
HVFI
AFCF
FLVF
FLIF
YCN
---RA
LWM
VCTV
LA--V
FIIC
FVPH
H----
--IT
LCLL
STN
CVLD
PVIY
CFLT
--O
poss
um-F
PIVY
SIIF
VLGF
VSN
AYVL
W--
---M
VNLT
VADL
LFLF
ALPL
WIV
YN
VAGC
LFFI
NTY
CSVC
FLGV
I---
-GIL
LSTI
IWVV
IVGC
ASYF
LI---
-----H
VFIV
LCFF
IVFL
VILV
CNVI
---L
WM
VCTV
MA-
-VFF
ICFV
PHHV
V---
QVT
LCLM
SIN
CVLD
PIIY
CFL-
--O
rang
utan
-FPI
VYSI
IFVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLVI
WVA
IVGA
ASYF
LIL-
----
-IHIF
IVFS
FFLV
FLIIL
FCN
L--
RALW
MVC
TVLA
--VFI
ICFV
PHH-
----
-VTL
CLLS
TNCV
LDPV
IYCF
LT--
Pig
-FPI
FYSI
IFVL
GVIA
NSY
VLW
-----
MLN
LTM
ADLL
FLVT
LPLW
IIYN
LAGC
FFFI
NTY
CSVS
FLAV
I---
--ISL
SLIIW
VAM
VAAA
SYFF
VL---
---IH
IFLV
FSFF
LVFL
VILF
CNL-
-RA
LWM
VCTV
LA--V
FIIC
FVPH
H----
--IT
LCLL
STN
CVLD
PIIY
CFLT
--Pi
ka-F
PIVY
SIIF
VLGV
VAN
GYVL
W--
--FM
VNLT
VADL
LFLI
TLPL
WII-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
---I
SLSL
IIWVV
ILAI
ASYF
LIM
-----
-IHIF
IVFC
FFVV
FLIIL
VCN
L--
RALW
MVC
TVLG
--VFI
ICFV
PHH-
----
QVT
LCLL
STN
CVLD
PVIY
CFL-
--Pl
atyp
us---
VAYS
IIFVL
GIFA
NGY
VLW
VF--F
MVN
LTVA
DLLF
LVTM
PLW
IV-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
--G
IVLS
LVIW
VLIV
SSAL
YFLV
------
-IHIF
IVAS
FFAV
FLLI
LVCN
L--
RALW
MVC
TVLA
--VFI
ICFV
PHH-
----
-VTL
CLLS
TNCV
LDPV
IYCF
LT--
Puffe
rfish
--PSA
YGVI
FILG
LLAN
LYVL
FI-
---M
TNLS
IADL
LFVC
ALPF
WIG
Y---
GSLF
FIN
TYCS
VLFL
GAIS
VN-G
IIISV
IIWVL
TISM
AIPY
LV---
------
FAIIG
LFFV
VFAL
VLVC
NLL
I---
--LLA
VVG-
-VFV
VCFL
PHHV
IQGA
QIT
LLLM
GLN
CILD
PVVY
CFA-
--Ra
bbit
--PIV
YSIIF
VLGV
VAN
GYVL
WV-
--FM
VNLT
VADL
LFLI
TLPL
WII-
NLA
GCLF
FIN
TYCS
VAFL
GVI--
---I
ALSL
VIW
VSIS
AAAT
YFLI
L----
--IH
IFIV
ASFF
IVFL
IILIC
NL-
-RA
LWM
VCTV
LA--V
FIIC
FVPH
H----
-Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Rat
--PIV
YSVI
FVLG
VVAN
GYVL
WV-
--FM
VNLT
VADL
LFLM
TLPL
WIV
-N
LAGC
LFFI
NTY
CSVA
FLGV
I---
-GIT
LSLV
IWIS
IAAT
ASYF
LA---
----V
HIFI
TSCF
FLVF
FLIF
YCN
M--
RALW
MVC
TVLA
--VFV
ICFV
PHH-
----
-ITLC
LLST
NCV
LDPV
IYCF
LT--
Shre
w-F
PIFY
SIIF
VLGV
IAN
SYVL
W--
--FM
VNLT
MAD
LLFL
ITLP
LWIV
-N
LTGC
FFFI
NTY
CSVA
FLGV
I---
-GIY
LSLV
IWM
AIVS
AASY
FFF-
-----
-IHIF
LVCS
FFLV
FLLI
FFCN
L--
RALW
MVC
TVLA
--VFS
ICFV
PHH-
----
-VTL
CLLS
TNCV
LDPI
IYCF
LT--
Squi
rrel
LFPI
IYSI
IFVV
GVIA
NGY
VL---
--FM
LNLT
VADL
LFLI
TLPL
WII-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
----
YLSL
VIW
VFIV
AAAS
YFLI
LD---
-VI
HIFI
VFSF
FLVF
LIIL
FCK-
--RA
LWM
VCAV
LA--V
FVIC
FVPH
H----
-Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Stickleba
ck-F
PVVY
SIIF
ILGI
TAN
LYVL
F--
CIYM
INLT
IGDL
LFVT
FLPF
W---
--SGS
LFFI
NTY
SSVL
FLCA
ISV-
-GIIL
SVFI
WLF
TLAT
AILF
LI---
------
QM
LFSV
VG--V
FVVC
FLPH
HACQ
----H
LTIV
IGFF
LVFF
VVVV
CNFL
-Q
ITW
LLM
SLN
CILD
PVVY
FFA-
--Zebrafi
sh--P
IFYG
VVFV
LGLI
ANCY
ALYV
--IY
MTN
LTVA
DLLF
VSAL
PFW
I-----
GTFF
FIN
TYGS
ILFL
TVIS
INRG
AIIS
VLIW
AFIL
SASI
PQL-
------
ITHF
SIIG
LFFL
VFLM
VIIC
N---
--LRM
LCAV
IG--V
FVIC
FLPH
HVV-
--Q
ITLM
LMGI
NCL
LDPL
VYCF
A---
7Supplementary data Search for PAF receptor in T. cruzi • Daniel Fábio Kawano et al.
Sequ
ence
Dom
ain
ID
omai
n II
Dom
ain
III
Dom
ain
IVD
omai
n V
Dom
ain
VI
Dom
ain
VII
Tc00
.104
7053
5097
99.1
00--H
LVGF
LAFL
VVVV
CF-F
VIVL
IYF
LFAA
YSFG
CLM
CMLC
STVF
---YF
GITF
LVVG
SFLP
FCYF
SFA-
---W
TYLS
MIS
FFGV
FGVL
GPFF
K--
LFYV
CMVG
SGLF
PIVH
IYLL
L----
-VE
GLLL
MM
ALYG
VGVF
VYAF
Q---
-----
IWHV
FVLA
A----
-AFV
HFFN
TASI
YHu
man
-FPI
VYSI
IFVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLVI
WVA
IVGA
ASYF
LIL-
-IHIF
IVFS
--FFL
VFLI
ILFC
NL-
--V
TLCL
LSTN
CVLD
PVIY
CFLT
----
-RAL
WM
VCTV
LAV-
-FIIC
FVPH
H----
-Ch
icke
n--T
VFYS
IIFIL
GFVA
NCY
VLW
I---F
MLN
LTVA
DLLF
LVTM
PLW
IV-
SVAG
SLFF
VNTY
ASVA
FLM
VI---
-GIF
LSAA
IWIIT
VGSA
LYYI
F--
AIHI
IICTL
--FYV
IFLF
ILTW
N---
-RAL
WM
VCTV
LVV-
-FIIS
FVPH
H----
--V
TLCL
LSM
NCV
LDPV
IYCF
LT---
-Ch
impa
nzee
-FPI
VYSI
IFVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
---I
SLSL
VIW
VAIV
GAAS
YFLI
L-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
-IHIF
IVFS
--FFL
VFLI
ILFC
NL-
--R
ALW
MVC
TVLA
V--F
IICFV
PHH-
----
-VTL
CLLS
TNCV
LDPV
IYCF
LT---
-Co
w-F
PIFY
SIVF
VLGV
IAN
SYVL
W--
---M
VNLT
MAD
LLFL
VTLP
LWIV
YN
LAGC
FFFI
NTY
CSVA
FLAV
I---
--ILL
SLIIW
VSIV
GAAS
YFFV
L--IH
IFLV
FS--F
FLVF
LIIL
FCN
L--
-RAL
WM
VCTV
LAV-
-FIIC
FVPH
H----
--V
TLCL
LSTN
CVLD
PIIY
CFLT
----
Elep
hant
-FPI
FYSI
IFVL
GVIA
NGY
VLW
-----
MVN
LTM
ADLL
FLVT
LPLW
IVY
NLA
GCLF
FIN
TYCS
VAFL
GVI--
---I
TLSL
VIW
VTIV
AAAS
YFLF
M-
--HIF
IVCS
--FFL
VFFI
ILVCN
LV-
-RAL
WM
VCTV
LAV-
-FIIC
FVPH
H----
-Q
VTLC
LLST
NCV
LDPV
IYCF
L----
-Fr
og---
VTYS
IIFVF
GFIA
NCY
VLW
IF-IF
MIN
LTIA
DLLF
LVTL
PLW
I--N
VAGC
FFFL
NTY
CSVA
FLGV
I---
--HFI
LVGA
--FFV
VFLI
ILTCN
LV-
---CI
SVAV
WIL
LFAS
SLYF
LIFP
-RAL
WM
VVTV
LAV-
-FVL
CFVP
HH---
-----
LCLM
STN
CMLD
PIIY
CFLT
KK--
Goat
-FPI
FYSI
VFVL
GVIA
NSY
VLW
-----
MVN
LTM
ADLL
FLVT
LPLW
IVY
NLA
GCFF
FIN
TYCS
VAFL
AVI--
---F
LLSL
IIWVS
IVGA
ASYF
FVL-
-IHIF
LVFS
--FFL
VFLI
ILFC
NL-
--R
ALW
MVC
TVLA
V--F
VICF
VPHH
-----
-VTL
CLLS
TNCV
LDPI
IYCF
LT---
-Gu
inea
pig
-FPI
VYSI
IFVL
GIIA
NGY
VLW
----F
MVN
LTVA
DLLF
LITL
PLW
IV-
NLA
GCLF
FIN
TYCS
VAFL
GVI--
---I
ALSL
VIW
VAIV
AAAS
YFLV
M-
-IHIC
IVLG
--FFI
VFLL
ILFC
NL-
--R
ALW
MVC
TVLA
V--F
VICF
VPHH
-----
QVT
LCLL
STN
CVLD
PVIY
CFL-
----
Hedg
ehog
-FPI
FYSI
IFAL
GVIA
NSY
VLW
----F
MVN
LTLA
DLLF
LVTL
PLW
II-N
LAGC
FFFI
NTY
CSVA
FLGV
I---
-GIIL
SLVI
WVA
IVSA
ASYF
FF--
-IHIF
LVVS
--FFV
VFLI
IFFC
NL-
--R
ALW
MVC
TVLA
V--F
IICFV
PHH-
----
QIT
LCLL
STN
CVLD
PIIY
CFL-
----
Hors
e--P
IVYS
IIFVL
GVIA
NSY
VLW
V--IF
MVN
LTVA
DLLF
LLTL
PLW
V--
NLT
GCCF
FIN
TYCS
VAFL
GVI--
---I
SLSL
VIW
LAIV
AAAS
YFLF
M-
-IHIF
LVFS
--FFL
VFLI
ILVCN
L--
-RAL
WM
VCTV
LAV-
-FIIC
FVPH
H----
-Q
VTLC
LLST
NCV
LDPI
IYCF
L----
-M
edak
a-F
SVVY
SIFF
VVGL
LAN
VYVL
F--
--YM
TNLT
IADL
LFVC
ALPF
WIG
---T
GSFF
FIN
TYGT
ILFL
GAIS
L-RG
IIVSV
LIW
LCLV
SSAI
PSL-
----H
FLIIG
L--F
FVVF
FLVM
VCN
LL-
----Q
MLL
AVVG
V--F
LLCF
LPHH
VVQ
--Q
ITLV
LMGL
NCI
LDPV
IYYF
A----
-M
onke
y-F
PIVY
SIIF
VLGV
IAN
GYVL
W--
--FM
VNLT
MAD
MLF
LITL
PLW
IV-
NLA
GCLF
FIN
TYCS
VAFL
GVI--
---I
SLSL
VIW
VAIV
GAAS
YFFI
L--IH
IFIV
FS--F
FLVF
LIIL
FCN
L--
-RAL
WM
VCTV
LAV-
-FIIC
FVPH
H----
-Q
VTLC
LLST
NCV
LDPV
IYCF
L----
-M
ouse
-FPI
VYSV
IFIL
GVVA
NGY
VLW
----F
MVN
LTM
ADLL
FLIT
LPLW
IV-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
--G
ISLS
LIIW
VSIV
ATAS
YFLA
--VV
HVFI
AFC-
-FFL
VFFL
IFYC
N---
-RAL
WM
VCTV
LAV-
-FIIC
FVPH
H----
--IT
LCLL
STN
CVLD
PVIY
CFLT
----
Opo
ssum
-FPI
VYSI
IFVL
GFVS
NAY
VLW
-----
MVN
LTVA
DLLF
LFAL
PLW
IVY
NVA
GCLF
FIN
TYCS
VCFL
GVI--
--G
ILLS
TIIW
VVIV
GCAS
YFLI
----H
VFIV
LC--F
FIVF
LVILV
CNVI
----
LWM
VCTV
MAV
--FFI
CFVP
HHVV
---Q
VTLC
LMSI
NCV
LDPI
IYCF
L----
-O
rang
utan
-FPI
VYSI
IFVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLVI
WVA
IVGA
ASYF
LIL-
-IHIF
IVFS
--FFL
VFLI
ILFC
NL-
--R
ALW
MVC
TVLA
V--F
IICFV
PHH-
----
-VTL
CLLS
TNCV
LDPV
IYCF
LT---
-Pi
g-F
PIFY
SIIF
VLGV
IAN
SYVL
W--
---M
LNLT
MAD
LLFL
VTLP
LWIIY
NLA
GCFF
FIN
TYCS
VSFL
AVI--
---I
SLSL
IIWVA
MVA
AASY
FFVL
--IH
IFLV
FS--F
FLVF
LVIL
FCN
L--
-RAL
WM
VCTV
LAV-
-FIIC
FVPH
H----
--IT
LCLL
STN
CVLD
PIIY
CFLT
----
Pika
-FPI
VYSI
IFVL
GVVA
NGY
VLW
----F
MVN
LTVA
DLLF
LITL
PLW
II-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLIIW
VVIL
AIAS
YFLI
M-
-IHIF
IVFC
--FFV
VFLI
ILVCN
L--
-RAL
WM
VCTV
LGV-
-FIIC
FVPH
H----
-Q
VTLC
LLST
NCV
LDPV
IYCF
L----
-Pl
atyp
us---
VAYS
IIFVL
GIFA
NGY
VLW
VF--F
MVN
LTVA
DLLF
LVTM
PLW
IV-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
--G
IVLS
LVIW
VLIV
SSAL
YFLV
---IH
IFIV
AS--F
FAVF
LLILV
CNL-
--R
ALW
MVC
TVLA
V--F
IICFV
PHH-
----
-VTL
CLLS
TNCV
LDPV
IYCF
LT---
-Pu
fferfish
--PSA
YGVI
FILG
LLAN
LYVL
FI-
---M
TNLS
IADL
LFVC
ALPF
WIG
Y---
GSLF
FIN
TYCS
VLFL
GAIS
VN-G
IIISV
IIWVL
TISM
AIPY
LV--
---FA
IIGL-
-FFV
VFAL
VLVC
NLL
I---
---LL
AVVG
V--F
VVCF
LPHH
VIQ
GAQ
ITLL
LMGL
NCI
LDPV
VYCF
A----
-Ra
bbit
--PIV
YSIIF
VLGV
VAN
GYVL
WV-
--FM
VNLT
VADL
LFLI
TLPL
WII-
NLA
GCLF
FIN
TYCS
VAFL
GVI--
---I
ALSL
VIW
VSIS
AAAT
YFLI
L--IH
IFIV
AS--F
FIVF
LIIL
ICN
L--
-RAL
WM
VCTV
LAV-
-FIIC
FVPH
H----
-Q
VTLC
LLST
NCV
LDPV
IYCF
L----
-Ra
t--P
IVYS
VIFV
LGVV
ANGY
VLW
V---F
MVN
LTVA
DLLF
LMTL
PLW
IV-
NLA
GCLF
FIN
TYCS
VAFL
GVI--
--G
ITLS
LVIW
ISIA
ATAS
YFLA
---R
ALW
MVC
TVLA
V--F
VICF
VPHH
-----
-VHI
FITS
C--F
FLVF
FLIF
YCN
M--
-ITLC
LLST
NCV
LDPV
IYCF
LT---
-Sh
rew
-FPI
FYSI
IFVL
GVIA
NSY
VLW
----F
MVN
LTM
ADLL
FLIT
LPLW
IV-
NLT
GCFF
FIN
TYCS
VAFL
GVI--
--G
IYLS
LVIW
MAI
VSAA
SYFF
F--
-IHIF
LVCS
--FFL
VFLL
IFFC
NL-
--R
ALW
MVC
TVLA
V--F
SICF
VPHH
-----
-VTL
CLLS
TNCV
LDPI
IYCF
LT---
-Sq
uirr
elLF
PIIY
SIIF
VVGV
IAN
GYVL
-----F
MLN
LTVA
DLLF
LITL
PLW
II-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
---YL
SLVI
WVF
IVAA
ASYF
LILD
VIHI
FIVF
S--F
FLVF
LIIL
FCK-
---R
ALW
MVC
AVLA
V--F
VICF
VPHH
-----
QVT
LCLL
STN
CVLD
PVIY
CFL-
----
Stickleba
ck-F
PVVY
SIIF
ILGI
TAN
LYVL
F--
CIYM
INLT
IGDL
LFVT
FLPF
W---
--SGS
LFFI
NTY
SSVL
FLCA
ISV-
-GIIL
SVFI
WLF
TLAT
AILF
LI--
--HLT
IVIG
--FFL
VFFV
VVVC
NFL
----
-QM
LFSV
VGV-
-FVV
CFLP
HHAC
Q--
QIT
WLL
MSL
NCI
LDPV
VYFF
A----
-Zebrafi
sh-IY
MTN
LTVA
DLLF
VSAL
PFW
I----P
IFYG
VVFV
LGLI
ANCY
ALYV
----
GTFF
FIN
TYGS
ILFL
TVIS
INRG
AIIS
VLIW
AFIL
SASI
PQL-
-----
LRM
LCAV
IGV-
-FVI
CFLP
HHVV
---IT
HFSI
IGL-
-FFL
VFLM
VIIC
N---
QIT
LMLM
GIN
CLLD
PLVY
CFA-
----
Sequ
ence
Dom
ain
ID
omai
n II
Dom
ain
III
Dom
ain
IVD
omai
n V
Dom
ain
VI
Dom
ain
VII
Tc00
.104
7053
5086
41.2
00---
-VYG
LLAA
GTAV
AGIG
SFLM
FS-
MPF
WLP
MVG
GFVP
LLW
LSFA
P----
---L
ALFF
SFTV
LEGM
ALAP
LVAI
-VL
GTAI
VLTG
AVFF
GFSA
AAL-
-----
-LQ
GPL-
FGM
LMGM
VAIS
ILN
LF-
FAHS
LILY
GGLA
LFSL
-FVS
VD---
--LQ
MFL
NVV
NIF
VRIA
QIL
GSM
----
Hum
an-F
PIVY
SIIF
VLGV
IA--N
GYVL
W--
--FM
VNLT
--MAD
MLF
LITL
PLW
IV-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
----
--ISL
SLVI
WVA
IVGA
ASYF
LIL-
-IHIF
IVFS
FFLV
FLIIL
FCN
L--
-RAL
WM
VCTV
LAVF
IICFV
PHH-
----
--VTL
CLLS
TNCV
LDPV
IYCF
LT--
Chic
ken
--FM
LNLT
--VAD
LLFL
VTM
PLW
IV-
--TVF
YSIIF
ILGF
VA--N
CYVL
WI-
SVAG
SLFF
VNTY
ASVA
FLM
VI---
----G
IFLS
AAIW
IITVG
SALY
YIF-
-AI
HIIIC
TLFY
VIFL
FILT
WN
-----V
TLCL
LSM
NCV
LDPV
IYCF
LT--
-RAL
WM
VCTV
LVVF
IISFV
PHH-
----
Chim
panz
ee-F
PIVY
SIIF
VLGV
IA--N
GYVL
W--
--FM
VNLT
--MAD
MLF
LITL
PLW
IV-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
----
--ISL
SLVI
WVA
IVGA
ASYF
LIL-
-IHIF
IVFS
FFLV
FLIIL
FCN
L--
-RAL
WM
VCTV
LAVF
IICFV
PHH-
----
--VTL
CLLS
TNCV
LDPV
IYCF
LT--
Cow
-FPI
FYSI
VFVL
GVIA
--NSY
VLW
--N
LAGC
FFFI
NTY
CSVA
FLAV
I---
---M
VNLT
--MAD
LLFL
VTLP
LWIV
Y---
--ILL
SLIIW
VSIV
GAAS
YFFV
L--IH
IFLV
FSFF
LVFL
IILFC
NL-
--R
ALW
MVC
TVLA
VFIIC
FVPH
H----
---V
TLCL
LSTN
CVLD
PIIY
CFLT
--El
epha
nt-F
PIFY
SIIF
VLGV
IA--N
GYVL
W--
---M
VNLT
--MAD
LLFL
VTLP
LWIV
YN
LAGC
LFFI
NTY
CSVA
FLGV
I---
-----I
TLSL
VIW
VTIV
AAAS
YFLF
M-
--HIF
IVCS
FFLV
FFIIL
VCN
LV-
-RAL
WM
VCTV
LAVF
IICFV
PHH-
----
-QVT
LCLL
STN
CVLD
PVIY
CFL-
--Fr
og---
VTYS
IIFVF
GFIA
--NCY
VLW
IF-IF
MIN
LT--I
ADLL
FLVT
LPLW
I--N
VAGC
FFFL
NTY
CSVA
FLGV
I---
------
CISV
AVW
ILLF
ASSL
YFLI
FP--H
FILV
GAFF
VVFL
IILTC
NLV
--R
ALW
MVV
TVLA
VFVL
CFVP
HH---
-----
-LCL
MST
NCM
LDPI
IYCF
LTKK
Goat
-FPI
FYSI
VFVL
GVIA
--NSY
VLW
-----
MVN
LT--M
ADLL
FLVT
LPLW
IVY
NLA
GCFF
FIN
TYCS
VAFL
AVI--
----
--FLL
SLIIW
VSIV
GAAS
YFFV
L--IH
IFLV
FSFF
LVFL
IILFC
NL-
--R
ALW
MVC
TVLA
VFVI
CFVP
HH---
----V
TLCL
LSTN
CVLD
PIIY
CFLT
--Gu
inea
pig
-FPI
VYSI
IFVL
GIIA
--NGY
VLW
----F
MVN
LT--V
ADLL
FLIT
LPLW
IV-
NLA
GCLF
FIN
TYCS
VAFL
GVI--
----
--IAL
SLVI
WVA
IVAA
ASYF
LVM
--IH
ICIV
LGFF
IVFL
LILF
CNL-
--R
ALW
MVC
TVLA
VFVI
CFVP
HH---
---Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Hedg
ehog
-FPI
FYSI
IFAL
GVIA
--NSY
VLW
----F
MVN
LT--L
ADLL
FLVT
LPLW
II-N
LAGC
FFFI
NTY
CSVA
FLGV
I---
----G
IILSL
VIW
VAIV
SAAS
YFFF
---IH
IFLV
VSFF
VVFL
IIFFC
NL-
--R
ALW
MVC
TVLA
VFIIC
FVPH
H----
--Q
ITLC
LLST
NCV
LDPI
IYCF
L---
Hors
e--P
IVYS
IIFVL
GVIA
--NSY
VLW
V--IF
MVN
LT--V
ADLL
FLLT
LPLW
V--
NLT
GCCF
FIN
TYCS
VAFL
GVI--
----
--ISL
SLVI
WLA
IVAA
ASYF
LFM
--IH
IFLV
FSFF
LVFL
IILVC
NL-
--R
ALW
MVC
TVLA
VFIIC
FVPH
H----
--Q
VTLC
LLST
NCV
LDPI
IYCF
L---
Med
aka
-FSV
VYSI
FFVV
GLLA
--NVY
VLF-
---Y
MTN
LT--I
ADLL
FVCA
LPFW
IG-
--TGS
FFFI
NTY
GTIL
FLGA
ISL-
---RG
IIVSV
LIW
LCLV
SSAI
PSL-
----H
FLIIG
LFFV
VFFL
VMVC
NLL
----
-QM
LLAV
VGVF
LLCF
LPHH
VVQ
---Q
ITLV
LMGL
NCI
LDPV
IYYF
A---
Mon
key
-FPI
VYSI
IFVL
GVIA
--NGY
VLW
----F
MVN
LT--M
ADM
LFLI
TLPL
WIV
-N
LAGC
LFFI
NTY
CSVA
FLGV
I---
-----I
SLSL
VIW
VAIV
GAAS
YFFI
L--IH
IFIV
FSFF
LVFL
IILFC
NL-
--Q
VTLC
LLST
NCV
LDPV
IYCF
L---
-RAL
WM
VCTV
LAVF
IICFV
PHH-
----
Mou
se-F
PIVY
SVIF
ILGV
VA--N
GYVL
W--
--FM
VNLT
--MAD
LLFL
ITLP
LWIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
----G
ISLS
LIIW
VSIV
ATAS
YFLA
--VV
HVFI
AFCF
FLVF
FLIF
YCN
----R
ALW
MVC
TVLA
VFIIC
FVPH
H----
---I
TLCL
LSTN
CVLD
PVIY
CFLT
--O
poss
um-F
PIVY
SIIF
VLGF
VS--N
AYVL
W--
---M
VNLT
--VAD
LLFL
FALP
LWIV
YN
VAGC
LFFI
NTY
CSVC
FLGV
I---
----G
ILLS
TIIW
VVIV
GCAS
YFLI
----H
VFIV
LCFF
IVFL
VILV
CNVI
----
LWM
VCTV
MAV
FFIC
FVPH
HVV-
---Q
VTLC
LMSI
NCV
LDPI
IYCF
L---
Ora
ngut
an-F
PIVY
SIIF
VLGV
IA--N
GYVL
W--
--FM
VNLT
--MAD
MLF
LITL
PLW
IV-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
----
--ISL
SLVI
WVA
IVGA
ASYF
LIL-
-IHIF
IVFS
FFLV
FLIIL
FCN
L--
-RAL
WM
VCTV
LAVF
IICFV
PHH-
----
--VTL
CLLS
TNCV
LDPV
IYCF
LT--
Pig
-FPI
FYSI
IFVL
GVIA
--NSY
VLW
-----
MLN
LT--M
ADLL
FLVT
LPLW
IIYN
LAGC
FFFI
NTY
CSVS
FLAV
I---
-IHIF
LVFS
FFLV
FLVI
LFCN
L--
-----I
SLSL
IIWVA
MVA
AASY
FFVL
--R
ALW
MVC
TVLA
VFIIC
FVPH
H----
---I
TLCL
LSTN
CVLD
PIIY
CFLT
--Pi
ka-F
PIVY
SIIF
VLGV
VA--N
GYVL
W--
--FM
VNLT
--VAD
LLFL
ITLP
LWII-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
----
--ISL
SLIIW
VVIL
AIAS
YFLI
M-
-IHIF
IVFC
FFVV
FLIIL
VCN
L--
-RAL
WM
VCTV
LGVF
IICFV
PHH-
----
-QVT
LCLL
STN
CVLD
PVIY
CFL-
--Pl
atyp
us---
VAYS
IIFVL
GIFA
--NGY
VLW
VF--F
MVN
LT--V
ADLL
FLVT
MPL
WIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
----G
IVLS
LVIW
VLIV
SSAL
YFLV
---IH
IFIV
ASFF
AVFL
LILV
CNL-
--R
ALW
MVC
TVLA
VFIIC
FVPH
H----
---V
TLCL
LSTN
CVLD
PVIY
CFLT
--Pu
fferfish
--PSA
YGVI
FILG
LLA-
-NLY
VLFI
----
MTN
LS--I
ADLL
FVCA
LPFW
IGY
---GS
LFFI
NTY
CSVL
FLGA
ISVN
----G
IIISV
IIWVL
TISM
AIPY
LV--
---FA
IIGLF
FVVF
ALVL
VCN
LLI
------
LLAV
VGVF
VVCF
LPHH
VIQ
GA-Q
ITLL
LMGL
NCI
LDPV
VYCF
A---
Rabb
it--P
IVYS
IIFVL
GVVA
--NGY
VLW
V---F
MVN
LT--V
ADLL
FLIT
LPLW
II-N
LAGC
LFFI
NTY
CSVA
FLGV
I---
-----I
ALSL
VIW
VSIS
AAAT
YFLI
L--IH
IFIV
ASFF
IVFL
IILIC
NL-
--R
ALW
MVC
TVLA
VFIIC
FVPH
H----
--Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Rat
--PIV
YSVI
FVLG
VVA-
-NGY
VLW
V---F
MVN
LT--V
ADLL
FLM
TLPL
WIV
-N
LAGC
LFFI
NTY
CSVA
FLGV
I---
----G
ITLS
LVIW
ISIA
ATAS
YFLA
---V
HIFI
TSCF
FLVF
FLIF
YCN
M--
-RAL
WM
VCTV
LAVF
VICF
VPHH
-----
--ITL
CLLS
TNCV
LDPV
IYCF
LT--
Shre
w-F
PIFY
SIIF
VLGV
IA--N
SYVL
W--
--FM
VNLT
--MAD
LLFL
ITLP
LWIV
-N
LTGC
FFFI
NTY
CSVA
FLGV
I---
----G
IYLS
LVIW
MAI
VSAA
SYFF
F--
-IHIF
LVCS
FFLV
FLLI
FFCN
L--
-RAL
WM
VCTV
LAVF
SICF
VPHH
-----
--VTL
CLLS
TNCV
LDPI
IYCF
LT--
Squi
rrel
LFPI
IYSI
IFVV
GVIA
--NGY
VL---
--FM
LNLT
--VAD
LLFL
ITLP
LWII-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
----
---YL
SLVI
WVF
IVAA
ASYF
LILD
VIHI
FIVF
SFFL
VFLI
ILFC
K---
-RAL
WM
VCAV
LAVF
VICF
VPHH
-----
-QVT
LCLL
STN
CVLD
PVIY
CFL-
--Sti
ckleba
ck-F
PVVY
SIIF
ILGI
TA--N
LYVL
F--
CIYM
INLT
--IGD
LLFV
TFLP
FW---
--SGS
LFFI
NTY
SSVL
FLCA
ISV-
----G
IILSV
FIW
LFTL
ATAI
LFLI
----H
LTIV
IGFF
LVFF
VVVV
CNFL
----
-QM
LFSV
VGVF
VVCF
LPHH
ACQ
---Q
ITW
LLM
SLN
CILD
PVVY
FFA-
--Zebrafi
sh-IY
MTN
LT--V
ADLL
FVSA
LPFW
I----P
IFYG
VVFV
LGLI
A--N
CYAL
YV-
---GT
FFFI
NTY
GSIL
FLTV
ISIN
---RG
AIIS
VLIW
AFIL
SASI
PQL-
-----
LRM
LCAV
IGVF
VICF
LPHH
VV---
ITHF
SIIG
LFFL
VFLM
VIIC
N---
-QIT
LMLM
GIN
CLLD
PLVY
CFA-
--
8Supplementary data Search for PAF receptor in T. cruzi • Daniel Fábio Kawano et al.
Sequ
ence
Dom
ain
ID
omai
n II
Dom
ain
III
Dom
ain
IVD
omai
n V
Dom
ain
VI
Dom
ain
VII
Tc00
.104
7053
5117
87.3
0GA
CEAH
LSM
AIAI
LFDL
LRSR
------
--DW
LMLG
LGGV
W---
-LPP
WSI
SFAL
FHCW
KALG
GKIL
FFFF
F---F
FME-
----
--STN
ALLQ
FLLA
VGGF
GVYS
NQ
--L
VIVA
SCM
TLTT
FLRI
SLCC
V--
MLL
IVVL
VFTA
VLDV
GNIL
LL---
--GV
SVTV
IFLG
ATAL
FGCV
IRL-
----
Hum
an---
-FPI
VYSI
IFVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
----
-----N
VAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLVI
WVA
IVGA
ASYF
LIL-
-IHIF
IVFS
FFLV
FLIIL
FCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
----
VTLC
LLST
NCV
LDPV
IYCF
LT--
Chic
ken
-----T
VFYS
IIFIL
GFVA
NCY
VLW
I---F
MLN
LTVA
DLLF
LVTM
PLW
IV---
----
--SVA
GSLF
FVN
TYAS
VAFL
MVI
----G
IFLS
AAIW
IITVG
SALY
YIF-
-AI
HIIIC
TLFY
VIFL
FILT
WN
---RA
LWM
VCTV
LVVF
IISFV
PHH-
----
---VT
LCLL
SMN
CVLD
PVIY
CFLT
--Ch
impa
nzee
----F
PIVY
SIIF
VLGV
IAN
GYVL
W--
--FM
VNLT
MAD
MLF
LITL
PLW
IV---
----
--NVA
GCLF
FIN
TYCS
VAFL
GVI--
---I
SLSL
VIW
VAIV
GAAS
YFLI
L--IH
IFIV
FSFF
LVFL
IILFC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
---VT
LCLL
STN
CVLD
PVIY
CFLT
--Co
w---
-FPI
FYSI
VFVL
GVIA
NSY
VLW
-----
MVN
LTM
ADLL
FLVT
LPLW
IVY-
-----
--NLA
GCFF
FIN
TYCS
VAFL
AVI--
---I
LLSL
IIWVS
IVGA
ASYF
FVL-
-IHIF
LVFS
FFLV
FLIIL
FCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
----
VTLC
LLST
NCV
LDPI
IYCF
LT--
Elep
hant
----F
PIFY
SIIF
VLGV
IAN
GYVL
W--
---M
VNLT
MAD
LLFL
VTLP
LWIV
Y---
-----N
LAGC
LFFI
NTY
CSVA
FLGV
I---
--ITL
SLVI
WVT
IVAA
ASYF
LFM
---H
IFIV
CSFF
LVFF
IILVC
NLV
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
--QVT
LCLL
STN
CVLD
PVIY
CFL-
--Fr
og---
---VT
YSIIF
VFGF
IAN
CYVL
WIF
-IFM
INLT
IADL
LFLV
TLPL
WI--
------
--NVA
GCFF
FLN
TYCS
VAFL
GVI--
----
CISV
AVW
ILLF
ASSL
YFLI
FP--H
FILV
GAFF
VVFL
IILTC
NLV
-RA
LWM
VVTV
LAVF
VLCF
VPHH
-----
-----L
CLM
STN
CMLD
PIIY
CFLT
KKGo
at---
-FPI
FYSI
VFVL
GVIA
NSY
VLW
-----
MVN
LTM
ADLL
FLVT
LPLW
IVY-
-----
--NLA
GCFF
FIN
TYCS
VAFL
AVI--
---F
LLSL
IIWVS
IVGA
ASYF
FVL-
-IHIF
LVFS
FFLV
FLIIL
FCN
L--
RALW
MVC
TVLA
VFVI
CFVP
HH---
-----
VTLC
LLST
NCV
LDPI
IYCF
LT--
Guin
ea p
ig---
-FPI
VYSI
IFVL
GIIA
NGY
VLW
----F
MVN
LTVA
DLLF
LITL
PLW
IV---
----
--NLA
GCLF
FIN
TYCS
VAFL
GVI--
---I
ALSL
VIW
VAIV
AAAS
YFLV
M-
-IHIC
IVLG
FFIV
FLLI
LFCN
L--
RALW
MVC
TVLA
VFVI
CFVP
HH---
----Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Hedg
ehog
----F
PIFY
SIIF
ALGV
IAN
SYVL
W--
--FM
VNLT
LADL
LFLV
TLPL
WII-
------
--NLA
GCFF
FIN
TYCS
VAFL
GVI--
--G
IILSL
VIW
VAIV
SAAS
YFFF
---IH
IFLV
VSFF
VVFL
IIFFC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
--QIT
LCLL
STN
CVLD
PIIY
CFL-
--Ho
rse
-----P
IVYS
IIFVL
GVIA
NSY
VLW
V--IF
MVN
LTVA
DLLF
LLTL
PLW
V----
----
--NLT
GCCF
FIN
TYCS
VAFL
GVI--
---I
SLSL
VIW
LAIV
AAAS
YFLF
M-
-IHIF
LVFS
FFLV
FLIIL
VCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
---Q
VTLC
LLST
NCV
LDPI
IYCF
L---
Med
aka
----F
SVVY
SIFF
VVGL
LAN
VYVL
F--
--YM
TNLT
IADL
LFVC
ALPF
WIG
----
------
-TGS
FFFI
NTY
GTIL
FLGA
ISL-
RGIIV
SVLI
WLC
LVSS
AIPS
L---
--HFL
IIGLF
FVVF
FLVM
VCN
LL-
---Q
MLL
AVVG
VFLL
CFLP
HHVV
Q--
--QIT
LVLM
GLN
CILD
PVIY
YFA-
--M
onke
y---
-FPI
VYSI
IFVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
----
-----N
LAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLVI
WVA
IVGA
ASYF
FIL-
-IHIF
IVFS
FFLV
FLIIL
FCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
---Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Mou
se---
-FPI
VYSV
IFIL
GVVA
NGY
VLW
----F
MVN
LTM
ADLL
FLIT
LPLW
IV---
----
--NVA
GCLF
FIN
TYCS
VAFL
GVI--
--G
ISLS
LIIW
VSIV
ATAS
YFLA
--VV
HVFI
AFCF
FLVF
FLIF
YCN
---RA
LWM
VCTV
LAVF
IICFV
PHH-
----
---IT
LCLL
STN
CVLD
PVIY
CFLT
--O
poss
um---
-FPI
VYSI
IFVL
GFVS
NAY
VLW
-----
MVN
LTVA
DLLF
LFAL
PLW
IVY-
-----
--NVA
GCLF
FIN
TYCS
VCFL
GVI--
--G
ILLS
TIIW
VVIV
GCAS
YFLI
----H
VFIV
LCFF
IVFL
VILV
CNVI
---L
WM
VCTV
MAV
FFIC
FVPH
HVV-
----Q
VTLC
LMSI
NCV
LDPI
IYCF
L---
Ora
ngut
an---
-FPI
VYSI
IFVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
----
-----N
VAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLVI
WVA
IVGA
ASYF
LIL-
-IHIF
IVFS
FFLV
FLIIL
FCN
L--
---VT
LCLL
STN
CVLD
PVIY
CFLT
--RA
LWM
VCTV
LAVF
IICFV
PHH-
----
Pig
----F
PIFY
SIIF
VLGV
IAN
SYVL
W--
---M
LNLT
MAD
LLFL
VTLP
LWIIY
------
--NLA
GCFF
FIN
TYCS
VSFL
AVI--
---I
SLSL
IIWVA
MVA
AASY
FFVL
--IH
IFLV
FSFF
LVFL
VILF
CNL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
---IT
LCLL
STN
CVLD
PIIY
CFLT
--Pi
ka---
-FPI
VYSI
IFVL
GVVA
NGY
VLW
----F
MVN
LTVA
DLLF
LITL
PLW
II----
-----N
VAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLIIW
VVIL
AIAS
YFLI
M-
-IHIF
IVFC
FFVV
FLIIL
VCN
L--
RALW
MVC
TVLG
VFIIC
FVPH
H----
---Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Plat
ypus
------
VAYS
IIFVL
GIFA
NGY
VLW
VF--F
MVN
LTVA
DLLF
LVTM
PLW
IV---
----
--NVA
GCLF
FIN
TYCS
VAFL
GVI--
--IH
IFIV
ASFF
AVFL
LILV
CNL-
--G
IVLS
LVIW
VLIV
SSAL
YFLV
--RA
LWM
VCTV
LAVF
IICFV
PHH-
----
---VT
LCLL
STN
CVLD
PVIY
CFLT
--Pu
fferfish
-----P
SAYG
VIFI
LGLL
ANLY
VLFI
----
MTN
LSIA
DLLF
VCAL
PFW
IGY-
-----
-----G
SLFF
INTY
CSVL
FLGA
ISVN
-GIII
SVIIW
VLTI
SMAI
PYLV
-----
FAIIG
LFFV
VFAL
VLVC
NLL
I---
--LLA
VVGV
FVVC
FLPH
HVIQ
GA--Q
ITLL
LMGL
NCI
LDPV
VYCF
A---
Rabb
it---
--PIV
YSIIF
VLGV
VAN
GYVL
WV-
--FM
VNLT
VADL
LFLI
TLPL
WII-
------
--NLA
GCLF
FIN
TYCS
VAFL
GVI--
---I
ALSL
VIW
VSIS
AAAT
YFLI
L--IH
IFIV
ASFF
IVFL
IILIC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
--QVT
LCLL
STN
CVLD
PVIY
CFL-
--Ra
t---
--PIV
YSVI
FVLG
VVAN
GYVL
WV-
--FM
VNLT
VADL
LFLM
TLPL
WIV
----
-----N
LAGC
LFFI
NTY
CSVA
FLGV
I---
-GIT
LSLV
IWIS
IAAT
ASYF
LA--
-VHI
FITS
CFFL
VFFL
IFYC
NM
--RA
LWM
VCTV
LAVF
VICF
VPHH
-----
---IT
LCLL
STN
CVLD
PVIY
CFLT
--Sh
rew
----F
PIFY
SIIF
VLGV
IAN
SYVL
W--
--FM
VNLT
MAD
LLFL
ITLP
LWIV
----
-----N
LTGC
FFFI
NTY
CSVA
FLGV
I---
-GIY
LSLV
IWM
AIVS
AASY
FFF-
--IH
IFLV
CSFF
LVFL
LIFF
CNL-
-RA
LWM
VCTV
LAVF
SICF
VPHH
-----
---VT
LCLL
STN
CVLD
PIIY
CFLT
--Sq
uirr
el---
LFPI
IYSI
IFVV
GVIA
NGY
VL---
--FM
LNLT
VADL
LFLI
TLPL
WII-
------
--NVA
GCLF
FIN
TYCS
VAFL
GVI--
----
YLSL
VIW
VFIV
AAAS
YFLI
LDVI
HIFI
VFSF
FLVF
LIIL
FCK-
--RA
LWM
VCAV
LAVF
VICF
VPHH
-----
--QVT
LCLL
STN
CVLD
PVIY
CFL-
--Sti
ckleba
ck---
-FPV
VYSI
IFIL
GITA
NLY
VLF-
-CI
YMIN
LTIG
DLLF
VTFL
PFW
------
------
-SGS
LFFI
NTY
SSVL
FLCA
ISV-
-GIIL
SVFI
WLF
TLAT
AILF
LI--
--HLT
IVIG
FFLV
FFVV
VVCN
FL-
---Q
MLF
SVVG
VFVV
CFLP
HHAC
Q--
--QIT
WLL
MSL
NCI
LDPV
VYFF
A---
Zebrafi
sh---
--PIF
YGVV
FVLG
LIAN
CYAL
YV-
-IYM
TNLT
VADL
LFVS
ALPF
WI--
------
-----G
TFFF
INTY
GSIL
FLTV
ISIN
RGAI
ISVL
IWAF
ILSA
SIPQ
L---
ITHF
SIIG
LFFL
VFLM
VIIC
N---
--LRM
LCAV
IGVF
VICF
LPHH
VV---
--QIT
LMLM
GIN
CLLD
PLVY
CFA-
--
Sequ
ence
Dom
ain
ID
omai
n II
Dom
ain
III
Dom
ain
IVD
omai
n V
Dom
ain
VI
Dom
ain
VII
Tc00
.104
7053
5103
05.7
9--T
TTLN
VVLP
AELL
YVVY
YLAY
----
-NAL
VASH
VALA
LFAS
LHVV
PA---
----Y
VPLV
MSL
VCIG
VLVL
CFVL
HSA
FLTY
LGFG
VLLY
GFAA
AAL-
-----
VFCL
RGLP
LVN
LLTG
VQLV
VSW
GAVQ
LLVI
VVLL
AIAV
LFYG
-----
ATFI
TIFF
IDVA
ALL-
-VFF
PVI--
-Hu
man
-FPI
VYSI
IFVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
--N
VAGC
LFFI
NTY
CSVA
FLGV
I-----
----I
SLSL
VIW
VAIV
GAAS
YFLI
L--IH
IFIV
FSFF
LVFL
IILFC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
---VT
LCLL
STN
CVLD
PVIY
CFLT
--Ch
icke
n--T
VFYS
IIFIL
GFVA
NCY
VLW
I---F
MLN
LTVA
DLLF
LVTM
PLW
IV--
SVAG
SLFF
VNTY
ASVA
FLM
VI---
----
-GIF
LSAA
IWIIT
VGSA
LYYI
F--
AIHI
IICTL
FYVI
FLFI
LTW
N---
RALW
MVC
TVLV
VFIIS
FVPH
H----
----
VTLC
LLSM
NCV
LDPV
IYCF
LT--
Chim
panz
ee-F
PIVY
SIIF
VLGV
IAN
GYVL
W--
--FM
VNLT
MAD
MLF
LITL
PLW
IV--
NVA
GCLF
FIN
TYCS
VAFL
GVI--
-----
--ISL
SLVI
WVA
IVGA
ASYF
LIL-
-IHIF
IVFS
FFLV
FLIIL
FCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
----
VTLC
LLST
NCV
LDPV
IYCF
LT--
Cow
-FPI
FYSI
VFVL
GVIA
NSY
VLW
-----
MVN
LTM
ADLL
FLVT
LPLW
IVY-
NLA
GCFF
FIN
TYCS
VAFL
AVI--
-----
--ILL
SLIIW
VSIV
GAAS
YFFV
L--IH
IFLV
FSFF
LVFL
IILFC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
---VT
LCLL
STN
CVLD
PIIY
CFLT
--El
epha
nt-F
PIFY
SIIF
VLGV
IAN
GYVL
W--
---M
VNLT
MAD
LLFL
VTLP
LWIV
Y-N
LAGC
LFFI
NTY
CSVA
FLGV
I-----
----I
TLSL
VIW
VTIV
AAAS
YFLF
M-
--HIF
IVCS
FFLV
FFIIL
VCN
LV-
RALW
MVC
TVLA
VFIIC
FVPH
H----
---Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Frog
---VT
YSIIF
VFGF
IAN
CYVL
WIF
-IFM
INLT
IADL
LFLV
TLPL
WI--
-N
VAGC
FFFL
NTY
CSVA
FLGV
I-----
-----
CISV
AVW
ILLF
ASSL
YFLI
FP--H
FILV
GAFF
VVFL
IILTC
NLV
-RA
LWM
VVTV
LAVF
VLCF
VPHH
-----
-----L
CLM
STN
CMLD
PIIY
CFLT
KKGo
at-F
PIFY
SIVF
VLGV
IAN
SYVL
W--
---M
VNLT
MAD
LLFL
VTLP
LWIV
Y-N
LAGC
FFFI
NTY
CSVA
FLAV
I-----
----F
LLSL
IIWVS
IVGA
ASYF
FVL-
-IHIF
LVFS
FFLV
FLIIL
FCN
L--
RALW
MVC
TVLA
VFVI
CFVP
HH---
-----
VTLC
LLST
NCV
LDPI
IYCF
LT--
Guin
ea p
ig-F
PIVY
SIIF
VLGI
IAN
GYVL
W--
--FM
VNLT
VADL
LFLI
TLPL
WIV
--N
LAGC
LFFI
NTY
CSVA
FLGV
I-----
----I
ALSL
VIW
VAIV
AAAS
YFLV
M-
-IHIC
IVLG
FFIV
FLLI
LFCN
L--
RALW
MVC
TVLA
VFVI
CFVP
HH---
----Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Hedg
ehog
-FPI
FYSI
IFAL
GVIA
NSY
VLW
----F
MVN
LTLA
DLLF
LVTL
PLW
II--
NLA
GCFF
FIN
TYCS
VAFL
GVI--
-----
-GIIL
SLVI
WVA
IVSA
ASYF
FF--
-IHIF
LVVS
FFVV
FLIIF
FCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
---Q
ITLC
LLST
NCV
LDPI
IYCF
L---
Hors
e--P
IVYS
IIFVL
GVIA
NSY
VLW
V--IF
MVN
LTVA
DLLF
LLTL
PLW
V---
NLT
GCCF
FIN
TYCS
VAFL
GVI--
-----
--ISL
SLVI
WLA
IVAA
ASYF
LFM
--IH
IFLV
FSFF
LVFL
IILVC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
--QVT
LCLL
STN
CVLD
PIIY
CFL-
--M
edak
a-F
SVVY
SIFF
VVGL
LAN
VYVL
F--
--YM
TNLT
IADL
LFVC
ALPF
WIG
----T
GSFF
FIN
TYGT
ILFL
GAIS
L----
-RG
IIVSV
LIW
LCLV
SSAI
PSL-
----H
FLIIG
LFFV
VFFL
VMVC
NLL
----
QM
LLAV
VGVF
LLCF
LPHH
VVQ
----Q
ITLV
LMGL
NCI
LDPV
IYYF
A---
Mon
key
-FPI
VYSI
IFVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
--N
LAGC
LFFI
NTY
CSVA
FLGV
I-----
----I
SLSL
VIW
VAIV
GAAS
YFFI
L--IH
IFIV
FSFF
LVFL
IILFC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
--QVT
LCLL
STN
CVLD
PVIY
CFL-
--M
ouse
-FPI
VYSV
IFIL
GVVA
NGY
VLW
----F
MVN
LTM
ADLL
FLIT
LPLW
IV--
NVA
GCLF
FIN
TYCS
VAFL
GVI--
-----
-GIS
LSLI
IWVS
IVAT
ASYF
LA--
VVHV
FIAF
CFFL
VFFL
IFYC
N---
RALW
MVC
TVLA
VFIIC
FVPH
H----
----
ITLC
LLST
NCV
LDPV
IYCF
LT--
Opo
ssum
-FPI
VYSI
IFVL
GFVS
NAY
VLW
-----
MVN
LTVA
DLLF
LFAL
PLW
IVY-
NVA
GCLF
FIN
TYCS
VCFL
GVI--
-----
-GIL
LSTI
IWVV
IVGC
ASYF
LI--
--HVF
IVLC
FFIV
FLVI
LVCN
VI-
--LW
MVC
TVM
AVFF
ICFV
PHHV
V---
--QVT
LCLM
SIN
CVLD
PIIY
CFL-
--O
rang
utan
-FPI
VYSI
IFVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
--N
VAGC
LFFI
NTY
CSVA
FLGV
I-----
----I
SLSL
VIW
VAIV
GAAS
YFLI
L--IH
IFIV
FSFF
LVFL
IILFC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
---VT
LCLL
STN
CVLD
PVIY
CFLT
--Pi
g-F
PIFY
SIIF
VLGV
IAN
SYVL
W--
---M
LNLT
MAD
LLFL
VTLP
LWIIY
-N
LAGC
FFFI
NTY
CSVS
FLAV
I-----
----I
SLSL
IIWVA
MVA
AASY
FFVL
--IH
IFLV
FSFF
LVFL
VILF
CNL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
---IT
LCLL
STN
CVLD
PIIY
CFLT
--Pi
ka-F
PIVY
SIIF
VLGV
VAN
GYVL
W--
--FM
VNLT
VADL
LFLI
TLPL
WII-
-N
VAGC
LFFI
NTY
CSVA
FLGV
I-----
----I
SLSL
IIWVV
ILAI
ASYF
LIM
--IH
IFIV
FCFF
VVFL
IILVC
NL-
-RA
LWM
VCTV
LGVF
IICFV
PHH-
----
--QVT
LCLL
STN
CVLD
PVIY
CFL-
--Pl
atyp
us---
VAYS
IIFVL
GIFA
NGY
VLW
VF--F
MVN
LTVA
DLLF
LVTM
PLW
IV--
NVA
GCLF
FIN
TYCS
VAFL
GVI--
-----
-GIV
LSLV
IWVL
IVSS
ALYF
LV--
-IHIF
IVAS
FFAV
FLLI
LVCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
----
VTLC
LLST
NCV
LDPV
IYCF
LT--
Puffe
rfish
--PSA
YGVI
FILG
LLAN
LYVL
FI-
---M
TNLS
IADL
LFVC
ALPF
WIG
Y----
GSLF
FIN
TYCS
VLFL
GAIS
VN---
--G
IIISV
IIWVL
TISM
AIPY
LV--
---FA
IIGLF
FVVF
ALVL
VCN
LLI
-----L
LAVV
GVFV
VCFL
PHHV
IQGA
--QIT
LLLM
GLN
CILD
PVVY
CFA-
--Ra
bbit
--PIV
YSIIF
VLGV
VAN
GYVL
WV-
--FM
VNLT
VADL
LFLI
TLPL
WII-
-N
LAGC
LFFI
NTY
CSVA
FLGV
I-----
----I
ALSL
VIW
VSIS
AAAT
YFLI
L--IH
IFIV
ASFF
IVFL
IILIC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
--QVT
LCLL
STN
CVLD
PVIY
CFL-
--Ra
t--P
IVYS
VIFV
LGVV
ANGY
VLW
V---F
MVN
LTVA
DLLF
LMTL
PLW
IV--
NLA
GCLF
FIN
TYCS
VAFL
GVI--
-----
-GIT
LSLV
IWIS
IAAT
ASYF
LA--
-VHI
FITS
CFFL
VFFL
IFYC
NM
--RA
LWM
VCTV
LAVF
VICF
VPHH
-----
---IT
LCLL
STN
CVLD
PVIY
CFLT
--Sh
rew
-FPI
FYSI
IFVL
GVIA
NSY
VLW
----F
MVN
LTM
ADLL
FLIT
LPLW
IV--
NLT
GCFF
FIN
TYCS
VAFL
GVI--
-----
-GIY
LSLV
IWM
AIVS
AASY
FFF-
--IH
IFLV
CSFF
LVFL
LIFF
CNL-
-RA
LWM
VCTV
LAVF
SICF
VPHH
-----
---VT
LCLL
STN
CVLD
PIIY
CFLT
--Sq
uirr
elLF
PIIY
SIIF
VVGV
IAN
GYVL
-----F
MLN
LTVA
DLLF
LITL
PLW
II--
NVA
GCLF
FIN
TYCS
VAFL
GVI--
-----
---YL
SLVI
WVF
IVAA
ASYF
LILD
VIHI
FIVF
SFFL
VFLI
ILFC
K---
RALW
MVC
AVLA
VFVI
CFVP
HH---
----Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Stickleba
ck-F
PVVY
SIIF
ILGI
TAN
LYVL
F--
CIYM
INLT
IGDL
LFVT
FLPF
W---
---S
GSLF
FIN
TYSS
VLFL
CAIS
V----
--G
IILSV
FIW
LFTL
ATAI
LFLI
----H
LTIV
IGFF
LVFF
VVVV
CNFL
----
QM
LFSV
VGVF
VVCF
LPHH
ACQ
----Q
ITW
LLM
SLN
CILD
PVVY
FFA-
--Zebrafi
sh--P
IFYG
VVFV
LGLI
ANCY
ALYV
--IY
MTN
LTVA
DLLF
VSAL
PFW
I---
---GT
FFFI
NTY
GSIL
FLTV
ISIN
----
RGAI
ISVL
IWAF
ILSA
SIPQ
L---
ITHF
SIIG
LFFL
VFLM
VIIC
N---
--LRM
LCAV
IGVF
VICF
LPHH
VV---
--QIT
LMLM
GIN
CLLD
PLVY
CFA-
--
9Supplementary data Search for PAF receptor in T. cruzi • Daniel Fábio Kawano et al.
Sequ
ence
Dom
ain
ID
omai
n II
Dom
ain
III
Dom
ain
IVD
omai
n V
Dom
ain
VI
Dom
ain
VII
Tc00
.104
7053
5108
61.9
4-F
KLVV
NCL
MLL
FLIL
IAVM
FH--
--HPR
FFFS
VALL
LLAV
FDIV
LR-
---HY
IAIV
ADTL
AVAY
VFTT
SVR
LESI
FLGL
TGLL
LSVN
LLSY
I----
-LGF
AMSV
APIF
FGFA
LFFC
IV--
MGF
SLVA
LYFV
MFG
DAIL
PAI--
---LI
VTVL
ANFM
TILF
ILFF
MM
T---
Hum
an-F
PIVY
SIIF
VLGV
IAN
GYVL
W--
--FM
VNLT
MAD
MLF
LITL
PLW
IV-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
----
ISLS
LVIW
VAIV
GAAS
YFLI
L--IH
IFIV
FSFF
LVFL
IILFC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
-VTL
CLLS
TNCV
LDPV
IYCF
LT--
Chic
ken
--TVF
YSIIF
ILGF
VAN
CYVL
WI-
--FM
LNLT
VADL
LFLV
TMPL
WIV
-SV
AGSL
FFVN
TYAS
VAFL
MVI
-----G
IFLS
AAIW
IITVG
SALY
YIF-
-AI
HIIIC
TLFY
VIFL
FILT
WN
---RA
LWM
VCTV
LVVF
IISFV
PHH-
----
-VTL
CLLS
MN
CVLD
PVIY
CFLT
--Ch
impa
nzee
-FPI
VYSI
IFVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
---IS
LSLV
IWVA
IVGA
ASYF
LIL-
-IHIF
IVFS
FFLV
FLIIL
FCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
--V
TLCL
LSTN
CVLD
PVIY
CFLT
--Co
w-F
PIFY
SIVF
VLGV
IAN
SYVL
W--
---M
VNLT
MAD
LLFL
VTLP
LWIV
YN
LAGC
FFFI
NTY
CSVA
FLAV
I---
---IL
LSLI
IWVS
IVGA
ASYF
FVL-
-IHIF
LVFS
FFLV
FLIIL
FCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
--V
TLCL
LSTN
CVLD
PIIY
CFLT
--El
epha
nt-F
PIFY
SIIF
VLGV
IAN
GYVL
W--
---M
VNLT
MAD
LLFL
VTLP
LWIV
YN
LAGC
LFFI
NTY
CSVA
FLGV
I---
---IT
LSLV
IWVT
IVAA
ASYF
LFM
---H
IFIV
CSFF
LVFF
IILVC
NLV
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
QVT
LCLL
STN
CVLD
PVIY
CFL-
--Fr
og---
VTYS
IIFVF
GFIA
NCY
VLW
IF-IF
MIN
LTIA
DLLF
LVTL
PLW
I--N
VAGC
FFFL
NTY
CSVA
FLGV
I---
----C
ISVA
VWIL
LFAS
SLYF
LIFP
--HFI
LVGA
FFVV
FLIIL
TCN
LV-
RALW
MVV
TVLA
VFVL
CFVP
HH---
-----
LCLM
STN
CMLD
PIIY
CFLT
KKGo
at-F
PIFY
SIVF
VLGV
IAN
SYVL
W--
---M
VNLT
MAD
LLFL
VTLP
LWIV
YN
LAGC
FFFI
NTY
CSVA
FLAV
I---
---FL
LSLI
IWVS
IVGA
ASYF
FVL-
-IHIF
LVFS
FFLV
FLIIL
FCN
L--
RALW
MVC
TVLA
VFVI
CFVP
HH---
---V
TLCL
LSTN
CVLD
PIIY
CFLT
--Gu
inea
pig
-FPI
VYSI
IFVL
GIIA
NGY
VLW
----F
MVN
LTVA
DLLF
LITL
PLW
IV-
NLA
GCLF
FIN
TYCS
VAFL
GVI--
----
IALS
LVIW
VAIV
AAAS
YFLV
M-
-IHIC
IVLG
FFIV
FLLI
LFCN
L--
RALW
MVC
TVLA
VFVI
CFVP
HH---
--Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Hedg
ehog
-FPI
FYSI
IFAL
GVIA
NSY
VLW
----F
MVN
LTLA
DLLF
LVTL
PLW
II-N
LAGC
FFFI
NTY
CSVA
FLGV
I---
--GIIL
SLVI
WVA
IVSA
ASYF
FF--
-IHIF
LVVS
FFVV
FLIIF
FCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
-Q
ITLC
LLST
NCV
LDPI
IYCF
L---
Hors
e--P
IVYS
IIFVL
GVIA
NSY
VLW
V--IF
MVN
LTVA
DLLF
LLTL
PLW
V--
NLT
GCCF
FIN
TYCS
VAFL
GVI--
----
ISLS
LVIW
LAIV
AAAS
YFLF
M-
-IHIF
LVFS
FFLV
FLIIL
VCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
-Q
VTLC
LLST
NCV
LDPI
IYCF
L---
Med
aka
-FSV
VYSI
FFVV
GLLA
NVY
VLF-
---Y
MTN
LTIA
DLLF
VCAL
PFW
IG-
--TGS
FFFI
NTY
GTIL
FLGA
ISL-
-RGI
IVSV
LIW
LCLV
SSAI
PSL-
----H
FLIIG
LFFV
VFFL
VMVC
NLL
----
QM
LLAV
VGVF
LLCF
LPHH
VVQ
--Q
ITLV
LMGL
NCI
LDPV
IYYF
A---
Mon
key
-FPI
VYSI
IFVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
-N
LAGC
LFFI
NTY
CSVA
FLGV
I---
---IS
LSLV
IWVA
IVGA
ASYF
FIL-
-IHIF
IVFS
FFLV
FLIIL
FCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
-Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Mou
se-F
PIVY
SVIF
ILGV
VAN
GYVL
W--
--FM
VNLT
MAD
LLFL
ITLP
LWIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
--GIS
LSLI
IWVS
IVAT
ASYF
LA--
VVHV
FIAF
CFFL
VFFL
IFYC
N---
RALW
MVC
TVLA
VFIIC
FVPH
H----
--IT
LCLL
STN
CVLD
PVIY
CFLT
--O
poss
um-F
PIVY
SIIF
VLGF
VSN
AYVL
W--
---M
VNLT
VADL
LFLF
ALPL
WIV
YN
VAGC
LFFI
NTY
CSVC
FLGV
I---
--HVF
IVLC
FFIV
FLVI
LVCN
VI-
--GIL
LSTI
IWVV
IVGC
ASYF
LI--
--LW
MVC
TVM
AVFF
ICFV
PHHV
V---
QVT
LCLM
SIN
CVLD
PIIY
CFL-
--O
rang
utan
-FPI
VYSI
IFVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
---IS
LSLV
IWVA
IVGA
ASYF
LIL-
-IHIF
IVFS
FFLV
FLIIL
FCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
--V
TLCL
LSTN
CVLD
PVIY
CFLT
--Pi
g-F
PIFY
SIIF
VLGV
IAN
SYVL
W--
---M
LNLT
MAD
LLFL
VTLP
LWIIY
NLA
GCFF
FIN
TYCS
VSFL
AVI--
----
ISLS
LIIW
VAM
VAAA
SYFF
VL-
-IHIF
LVFS
FFLV
FLVI
LFCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
--IT
LCLL
STN
CVLD
PIIY
CFLT
--Pi
ka-F
PIVY
SIIF
VLGV
VAN
GYVL
W--
--FM
VNLT
VADL
LFLI
TLPL
WII-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
----
ISLS
LIIW
VVIL
AIAS
YFLI
M-
-IHIF
IVFC
FFVV
FLIIL
VCN
L--
RALW
MVC
TVLG
VFIIC
FVPH
H----
-Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Plat
ypus
---VA
YSIIF
VLGI
FAN
GYVL
WVF
--FM
VNLT
VADL
LFLV
TMPL
WIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
--GIV
LSLV
IWVL
IVSS
ALYF
LV--
-IHIF
IVAS
FFAV
FLLI
LVCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
--V
TLCL
LSTN
CVLD
PVIY
CFLT
--Pu
fferfish
--PSA
YGVI
FILG
LLAN
LYVL
FI-
---M
TNLS
IADL
LFVC
ALPF
WIG
Y---
GSLF
FIN
TYCS
VLFL
GAIS
VN--G
IIISV
IIWVL
TISM
AIPY
LV--
---FA
IIGLF
FVVF
ALVL
VCN
LLI
-----L
LAVV
GVFV
VCFL
PHHV
IQGA
QIT
LLLM
GLN
CILD
PVVY
CFA-
--Ra
bbit
--PIV
YSIIF
VLGV
VAN
GYVL
WV-
--FM
VNLT
VADL
LFLI
TLPL
WII-
NLA
GCLF
FIN
TYCS
VAFL
GVI--
----
IALS
LVIW
VSIS
AAAT
YFLI
L--IH
IFIV
ASFF
IVFL
IILIC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
QVT
LCLL
STN
CVLD
PVIY
CFL-
--Ra
t--P
IVYS
VIFV
LGVV
ANGY
VLW
V---F
MVN
LTVA
DLLF
LMTL
PLW
IV-
NLA
GCLF
FIN
TYCS
VAFL
GVI--
---G
ITLS
LVIW
ISIA
ATAS
YFLA
---V
HIFI
TSCF
FLVF
FLIF
YCN
M--
RALW
MVC
TVLA
VFVI
CFVP
HH---
---IT
LCLL
STN
CVLD
PVIY
CFLT
--Sh
rew
-FPI
FYSI
IFVL
GVIA
NSY
VLW
----F
MVN
LTM
ADLL
FLIT
LPLW
IV-
NLT
GCFF
FIN
TYCS
VAFL
GVI--
---G
IYLS
LVIW
MAI
VSAA
SYFF
F--
-IHIF
LVCS
FFLV
FLLI
FFCN
L--
RALW
MVC
TVLA
VFSI
CFVP
HH---
---V
TLCL
LSTN
CVLD
PIIY
CFLT
--Sq
uirr
elLF
PIIY
SIIF
VVGV
IAN
GYVL
-----F
MLN
LTVA
DLLF
LITL
PLW
II-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
----Y
LSLV
IWVF
IVAA
ASYF
LILD
VIHI
FIVF
SFFL
VFLI
ILFC
K---
RALW
MVC
AVLA
VFVI
CFVP
HH---
--Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Stickleba
ck-F
PVVY
SIIF
ILGI
TAN
LYVL
F--
CIYM
INLT
IGDL
LFVT
FLPF
W---
--SGS
LFFI
NTY
SSVL
FLCA
ISV-
--GIIL
SVFI
WLF
TLAT
AILF
LI--
---Q
MLF
SVVG
VFVV
CFLP
HHAC
Q--
--HLT
IVIG
FFLV
FFVV
VVCN
FL-
QIT
WLL
MSL
NCI
LDPV
VYFF
A---
Zebrafi
sh--P
IFYG
VVFV
LGLI
ANCY
ALYV
--IY
MTN
LTVA
DLLF
VSAL
PFW
I-----
GTFF
FIN
TYGS
ILFL
TVIS
IN-R
GAIIS
VLIW
AFIL
SASI
PQL-
--IT
HFSI
IGLF
FLVF
LMVI
ICN
-----L
RMLC
AVIG
VFVI
CFLP
HHVV
---Q
ITLM
LMGI
NCL
LDPL
VYCF
A---
Sequ
ence
Dom
ain
ID
omai
n II
Dom
ain
III
Dom
ain
IVD
omai
n V
Dom
ain
VI
Dom
ain
VII
Tc00
.104
7053
5119
23.7
1-G
DV--A
IIMAA
PSLT
ALSV
AIRM
------
-GLA
ELLA
FALS
PAVI
IAHA
LR---
VFVL
LIPR
WPI
RMYL
SVAW
RVTL
FFCG
VAVA
IPSL
CTW
CSFL
---YL
FLLG
VLCV
WLS
PFSP
I-CLL
--GQ
PRLP
ICW
TLIL
CCAW
GMLS
------
------
VVVA
VAVF
LFLP
TGVR
CVL-
-----F
L---
Hum
an-F
PIVY
SIIF
VLGV
IAN
GYVL
W--
--FM
VNLT
MAD
MLF
LITL
PLW
IV---
--N
VAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLVI
WVA
IVGA
ASYF
LIL-
-IHIF
IVFS
FFLV
FLIIL
FCN
L--
-----R
ALW
--MVC
TVLA
VFIIC
FVPH
H----
----
-VTL
CLL-
--STN
CVLD
PVIY
CFLT
--Ch
icke
n--T
VFYS
IIFIL
GFVA
NCY
VLW
I---F
MLN
LTVA
DLLF
LVTM
PLW
IV---
--SV
AGSL
FFVN
TYAS
VAFL
MVI
----G
IFLS
AAIW
IITVG
SALY
YIF-
-AI
HIIIC
TLFY
VIFL
FILT
WN
------
--RAL
W--M
VCTV
LVVF
IISFV
PHH-
----
----V
TLCL
L---S
MN
CVLD
PVIY
CFLT
--Ch
impa
nzee
-FPI
VYSI
IFVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
-----
NVA
GCLF
FIN
TYCS
VAFL
GVI--
---I
SLSL
VIW
VAIV
GAAS
YFLI
L--IH
IFIV
FSFF
LVFL
IILFC
NL-
----
--RAL
W--M
VCTV
LAVF
IICFV
PHH-
----
----V
TLCL
L---S
TNCV
LDPV
IYCF
LT--
Cow
-FPI
FYSI
VFVL
GVIA
NSY
VLW
-----
MVN
LTM
ADLL
FLVT
LPLW
IVY-
---N
LAGC
FFFI
NTY
CSVA
FLAV
I---
--ILL
SLIIW
VSIV
GAAS
YFFV
L--IH
IFLV
FSFF
LVFL
IILFC
NL-
----
--RAL
W--M
VCTV
LAVF
IICFV
PHH-
----
----V
TLCL
L---S
TNCV
LDPI
IYCF
LT--
Elep
hant
-FPI
FYSI
IFVL
GVIA
NGY
VLW
-----
MVN
LTM
ADLL
FLVT
LPLW
IVY-
---N
LAGC
LFFI
NTY
CSVA
FLGV
I---
--ITL
SLVI
WVT
IVAA
ASYF
LFM
---H
IFIV
CSFF
LVFF
IILVC
NLV
----
--RAL
W--M
VCTV
LAVF
IICFV
PHH-
----
---Q
VTLC
LL---
STN
CVLD
PVIY
CFL-
--Fr
og---
VTYS
IIFVF
GFIA
NCY
VLW
IF-IF
MIN
LTIA
DLLF
LVTL
PLW
I-----
-N
VAGC
FFFL
NTY
CSVA
FLGV
I---
---CI
SVAV
WIL
LFAS
SLYF
LIFP
--HFI
LVGA
FFVV
FLIIL
TCN
LV-
-----R
ALW
--MVV
TVLA
VFVL
CFVP
HH---
-----
---LC
LM---
STN
CMLD
PIIY
CFLT
KKGo
at-F
PIFY
SIVF
VLGV
IAN
SYVL
W--
---M
VNLT
MAD
LLFL
VTLP
LWIV
Y----
NLA
GCFF
FIN
TYCS
VAFL
AVI--
---F
LLSL
IIWVS
IVGA
ASYF
FVL-
-IHIF
LVFS
FFLV
FLIIL
FCN
L--
-----R
ALW
--MVC
TVLA
VFVI
CFVP
HH---
-----
-VTL
CLL-
--STN
CVLD
PIIY
CFLT
--Gu
inea
pig
-FPI
VYSI
IFVL
GIIA
NGY
VLW
----F
MVN
LTVA
DLLF
LITL
PLW
IV---
--N
LAGC
LFFI
NTY
CSVA
FLGV
I---
--IAL
SLVI
WVA
IVAA
ASYF
LVM
--IH
ICIV
LGFF
IVFL
LILF
CNL-
----
--RAL
W--M
VCTV
LAVF
VICF
VPHH
-----
---Q
VTLC
LL---
STN
CVLD
PVIY
CFL-
--He
dgeh
og-F
PIFY
SIIF
ALGV
IAN
SYVL
W--
--FM
VNLT
LADL
LFLV
TLPL
WII-
----
NLA
GCFF
FIN
TYCS
VAFL
GVI--
--G
IILSL
VIW
VAIV
SAAS
YFFF
---IH
IFLV
VSFF
VVFL
IIFFC
NL-
----
--RAL
W--M
VCTV
LAVF
IICFV
PHH-
----
---Q
ITLC
LL---
STN
CVLD
PIIY
CFL-
--Ho
rse
--PIV
YSIIF
VLGV
IAN
SYVL
WV-
-IFM
VNLT
VADL
LFLL
TLPL
WV-
-----
NLT
GCCF
FIN
TYCS
VAFL
GVI--
---I
SLSL
VIW
LAIV
AAAS
YFLF
M-
-IHIF
LVFS
FFLV
FLIIL
VCN
L--
-----R
ALW
--MVC
TVLA
VFIIC
FVPH
H----
----
QVT
LCLL
---ST
NCV
LDPI
IYCF
L---
Med
aka
-FSV
VYSI
FFVV
GLLA
NVY
VLF-
---Y
MTN
LTIA
DLLF
VCAL
PFW
IG---
----T
GSFF
FIN
TYGT
ILFL
GAIS
L-RG
IIVSV
LIW
LCLV
SSAI
PSL-
----H
FLIIG
LFFV
VFFL
VMVC
NLL
----
-----Q
--MLL
AVVG
VFLL
CFLP
HHVV
Q--
---Q
ITLV
LM---
GLN
CILD
PVIY
YFA-
--M
onke
y-F
PIVY
SIIF
VLGV
IAN
GYVL
W--
--FM
VNLT
MAD
MLF
LITL
PLW
IV---
--N
LAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLVI
WVA
IVGA
ASYF
FIL-
-IHIF
IVFS
FFLV
FLIIL
FCN
L--
-----R
ALW
--MVC
TVLA
VFIIC
FVPH
H----
----
QVT
LCLL
---ST
NCV
LDPV
IYCF
L---
Mou
se-F
PIVY
SVIF
ILGV
VAN
GYVL
W--
--FM
VNLT
MAD
LLFL
ITLP
LWIV
-----
NVA
GCLF
FIN
TYCS
VAFL
GVI--
--G
ISLS
LIIW
VSIV
ATAS
YFLA
--VV
HVFI
AFCF
FLVF
FLIF
YCN
------
--RAL
W--M
VCTV
LAVF
IICFV
PHH-
----
----IT
LCLL
---ST
NCV
LDPV
IYCF
LT--
Opo
ssum
-FPI
VYSI
IFVL
GFVS
NAY
VLW
-----
MVN
LTVA
DLLF
LFAL
PLW
IVY-
---N
VAGC
LFFI
NTY
CSVC
FLGV
I---
-GIL
LSTI
IWVV
IVGC
ASYF
LI--
--HVF
IVLC
FFIV
FLVI
LVCN
VI-
------
-LW
--MVC
TVM
AVFF
ICFV
PHHV
V---
---Q
VTLC
LM---
SIN
CVLD
PIIY
CFL-
--O
rang
utan
-FPI
VYSI
IFVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
-----
NVA
GCLF
FIN
TYCS
VAFL
GVI--
---I
SLSL
VIW
VAIV
GAAS
YFLI
L--IH
IFIV
FSFF
LVFL
IILFC
NL-
----
--RAL
W--M
VCTV
LAVF
IICFV
PHH-
----
----V
TLCL
L---S
TNCV
LDPV
IYCF
LT--
Pig
-FPI
FYSI
IFVL
GVIA
NSY
VLW
-----
MLN
LTM
ADLL
FLVT
LPLW
IIY---
-N
LAGC
FFFI
NTY
CSVS
FLAV
I---
--ISL
SLIIW
VAM
VAAA
SYFF
VL-
-IHIF
LVFS
FFLV
FLVI
LFCN
L--
-----R
ALW
--MVC
TVLA
VFIIC
FVPH
H----
----
-ITLC
LL---
STN
CVLD
PIIY
CFLT
--Pi
ka-F
PIVY
SIIF
VLGV
VAN
GYVL
W--
--FM
VNLT
VADL
LFLI
TLPL
WII-
----
NVA
GCLF
FIN
TYCS
VAFL
GVI--
---I
SLSL
IIWVV
ILAI
ASYF
LIM
--IH
IFIV
FCFF
VVFL
IILVC
NL-
----
--RAL
W--M
VCTV
LGVF
IICFV
PHH-
----
---Q
VTLC
LL---
STN
CVLD
PVIY
CFL-
--Pl
atyp
us---
VAYS
IIFVL
GIFA
NGY
VLW
VF--F
MVN
LTVA
DLLF
LVTM
PLW
IV---
--N
VAGC
LFFI
NTY
CSVA
FLGV
I---
-GIV
LSLV
IWVL
IVSS
ALYF
LV--
-IHIF
IVAS
FFAV
FLLI
LVCN
L--
-----R
ALW
--MVC
TVLA
VFIIC
FVPH
H----
----
-VTL
CLL-
--STN
CVLD
PVIY
CFLT
--Pu
fferfish
--PSA
YGVI
FILG
LLAN
LYVL
FI-
---M
TNLS
IADL
LFVC
ALPF
WIG
Y----
---GS
LFFI
NTY
CSVL
FLGA
ISVN
-GIII
SVIIW
VLTI
SMAI
PYLV
-----
FAIIG
LFFV
VFAL
VLVC
NLL
I---
------
---LL
AVVG
VFVV
CFLP
HHVI
QGA
---Q
ITLL
LM---
GLN
CILD
PVVY
CFA-
--Ra
bbit
--PIV
YSIIF
VLGV
VAN
GYVL
WV-
--FM
VNLT
VADL
LFLI
TLPL
WII-
----
NLA
GCLF
FIN
TYCS
VAFL
GVI--
---I
ALSL
VIW
VSIS
AAAT
YFLI
L--IH
IFIV
ASFF
IVFL
IILIC
NL-
----
--RAL
W--M
VCTV
LAVF
IICFV
PHH-
----
---Q
VTLC
LL---
STN
CVLD
PVIY
CFL-
--Ra
t--P
IVYS
VIFV
LGVV
ANGY
VLW
V---F
MVN
LTVA
DLLF
LMTL
PLW
IV---
--N
LAGC
LFFI
NTY
CSVA
FLGV
I---
-GIT
LSLV
IWIS
IAAT
ASYF
LA--
-VHI
FITS
CFFL
VFFL
IFYC
NM
-----
--RAL
W--M
VCTV
LAVF
VICF
VPHH
-----
----IT
LCLL
---ST
NCV
LDPV
IYCF
LT--
Shre
w-F
PIFY
SIIF
VLGV
IAN
SYVL
W--
--FM
VNLT
MAD
LLFL
ITLP
LWIV
-----
NLT
GCFF
FIN
TYCS
VAFL
GVI--
--G
IYLS
LVIW
MAI
VSAA
SYFF
F--
-IHIF
LVCS
FFLV
FLLI
FFCN
L--
-----R
ALW
--MVC
TVLA
VFSI
CFVP
HH---
-----
-VTL
CLL-
--STN
CVLD
PIIY
CFLT
--Sq
uirr
elLF
PIIY
SIIF
VVGV
IAN
GYVL
-----F
MLN
LTVA
DLLF
LITL
PLW
II----
-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
---YL
SLVI
WVF
IVAA
ASYF
LILD
VIHI
FIVF
SFFL
VFLI
ILFC
K---
-----R
ALW
--MVC
AVLA
VFVI
CFVP
HH---
-----
QVT
LCLL
---ST
NCV
LDPV
IYCF
L---
Stickleba
ck-F
PVVY
SIIF
ILGI
TAN
LYVL
F--
CIYM
INLT
IGDL
LFVT
FLPF
W---
----
--SGS
LFFI
NTY
SSVL
FLCA
ISV-
-GIIL
SVFI
WLF
TLAT
AILF
LI--
--HLT
IVIG
FFLV
FFVV
VVCN
FL-
------
--Q--M
LFSV
VGVF
VVCF
LPHH
ACQ
-----
QIT
WLL
M---
SLN
CILD
PVVY
FFA-
--Zebrafi
sh--P
IFYG
VVFV
LGLI
ANCY
ALYV
--IY
MTN
LTVA
DLLF
VSAL
PFW
I-----
----
GTFF
FIN
TYGS
ILFL
TVIS
INRG
AIIS
VLIW
AFIL
SASI
PQL-
--IT
HFSI
IGLF
FLVF
LMVI
ICN
------
----L
R--M
LCAV
IGVF
VICF
LPHH
VV---
---Q
ITLM
LM---
GIN
CLLD
PLVY
CFA-
--
10Supplementary data Search for PAF receptor in T. cruzi • Daniel Fábio Kawano et al.
Sequ
ence
Dom
ain
ID
omai
n II
Dom
ain
III
Dom
ain
IVD
omai
n V
Dom
ain
VI
Dom
ain
VII
Tc00
.104
7053
5119
21.7
1TL
FFCG
VAVA
-ILSL
CT---
WCG
FL-V
SGRR
SLIC
FTCG
M--V
ETLP
AT-A
MRC
CSGV
FCFL
EAFI
SFPS
C--
-SFT
LCVG
SVTL
SCVI
HAVC
TF--
VAVP
RLLW
TGCL
TAVF
MEL
LW---
------
CGSL
LLLF
YGGC
SV-V
VCAC
FL---
-GDV
--AIIM
AAPS
LTAL
SVAI
RMHu
man
-FPI
VYSI
IFVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
--N
VAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLVI
WVA
IVGA
ASYF
LIL-
-IHIF
IVFS
FFLV
FLIIL
FCN
L--
----R
ALW
MVC
TVLA
VFIIC
FVPH
H----
---V
TLCL
LSTN
CVLD
PVIY
CFLT
--Ch
icke
n--T
VFYS
IIFIL
GFVA
NCY
VLW
I---F
MLN
LTVA
DLLF
LVTM
PLW
IV--
SVAG
SLFF
VNTY
ASVA
FLM
VI---
-GIF
LSAA
IWIIT
VGSA
LYYI
F--
AIHI
IICTL
FYVI
FLFI
LTW
N---
----R
ALW
MVC
TVLV
VFIIS
FVPH
H----
---V
TLCL
LSM
NCV
LDPV
IYCF
LT--
Chim
panz
ee-F
PIVY
SIIF
VLGV
IAN
GYVL
W--
--FM
VNLT
MAD
MLF
LITL
PLW
IV--
NVA
GCLF
FIN
TYCS
VAFL
GVI--
---I
SLSL
VIW
VAIV
GAAS
YFLI
L--IH
IFIV
FSFF
LVFL
IILFC
NL-
----
-RAL
WM
VCTV
LAVF
IICFV
PHH-
----
--VTL
CLLS
TNCV
LDPV
IYCF
LT--
Cow
-FPI
FYSI
VFVL
GVIA
NSY
VLW
-----
MVN
LTM
ADLL
FLVT
LPLW
IVY-
NLA
GCFF
FIN
TYCS
VAFL
AVI--
---I
LLSL
IIWVS
IVGA
ASYF
FVL-
-IHIF
LVFS
FFLV
FLIIL
FCN
L--
----R
ALW
MVC
TVLA
VFIIC
FVPH
H----
---V
TLCL
LSTN
CVLD
PIIY
CFLT
--El
epha
nt-F
PIFY
SIIF
VLGV
IAN
GYVL
W--
---M
VNLT
MAD
LLFL
VTLP
LWIV
Y-N
LAGC
LFFI
NTY
CSVA
FLGV
I---
--ITL
SLVI
WVT
IVAA
ASYF
LFM
---H
IFIV
CSFF
LVFF
IILVC
NLV
----
-RAL
WM
VCTV
LAVF
IICFV
PHH-
----
-QVT
LCLL
STN
CVLD
PVIY
CFL-
--Fr
og---
VTYS
IIFVF
GFIA
NCY
VLW
IF-IF
MIN
LTIA
DLLF
LVTL
PLW
I---
NVA
GCFF
FLN
TYCS
VAFL
GVI--
----
CISV
AVW
ILLF
ASSL
YFLI
FP--H
FILV
GAFF
VVFL
IILTC
NLV
----
-RAL
WM
VVTV
LAVF
VLCF
VPHH
-----
----L
CLM
STN
CMLD
PIIY
CFLT
KKGo
at-F
PIFY
SIVF
VLGV
IAN
SYVL
W--
---M
VNLT
MAD
LLFL
VTLP
LWIV
Y-N
LAGC
FFFI
NTY
CSVA
FLAV
I---
--FLL
SLIIW
VSIV
GAAS
YFFV
L--IH
IFLV
FSFF
LVFL
IILFC
NL-
----
-RAL
WM
VCTV
LAVF
VICF
VPHH
-----
--VTL
CLLS
TNCV
LDPI
IYCF
LT--
Guin
ea p
ig-F
PIVY
SIIF
VLGI
IAN
GYVL
W--
--FM
VNLT
VADL
LFLI
TLPL
WIV
--N
LAGC
LFFI
NTY
CSVA
FLGV
I---
--IAL
SLVI
WVA
IVAA
ASYF
LVM
--IH
ICIV
LGFF
IVFL
LILF
CNL-
----
-RAL
WM
VCTV
LAVF
VICF
VPHH
-----
-QVT
LCLL
STN
CVLD
PVIY
CFL-
--He
dgeh
og-F
PIFY
SIIF
ALGV
IAN
SYVL
W--
--FM
VNLT
LADL
LFLV
TLPL
WII-
-N
LAGC
FFFI
NTY
CSVA
FLGV
I---
-GIIL
SLVI
WVA
IVSA
ASYF
FF--
-IHIF
LVVS
FFVV
FLIIF
FCN
L--
----R
ALW
MVC
TVLA
VFIIC
FVPH
H----
--Q
ITLC
LLST
NCV
LDPI
IYCF
L---
Hors
e--P
IVYS
IIFVL
GVIA
NSY
VLW
V--IF
MVN
LTVA
DLLF
LLTL
PLW
V---
NLT
GCCF
FIN
TYCS
VAFL
GVI--
---I
SLSL
VIW
LAIV
AAAS
YFLF
M-
-IHIF
LVFS
FFLV
FLIIL
VCN
L--
----R
ALW
MVC
TVLA
VFIIC
FVPH
H----
--Q
VTLC
LLST
NCV
LDPI
IYCF
L---
Med
aka
-FSV
VYSI
FFVV
GLLA
NVY
VLF-
---Y
MTN
LTIA
DLLF
VCAL
PFW
IG--
--TGS
FFFI
NTY
GTIL
FLGA
ISL-
RGIIV
SVLI
WLC
LVSS
AIPS
L---
--HFL
IIGLF
FVVF
FLVM
VCN
LL-
------
-QM
LLAV
VGVF
LLCF
LPHH
VVQ
---Q
ITLV
LMGL
NCI
LDPV
IYYF
A---
Mon
key
-FPI
VYSI
IFVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
--N
LAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLVI
WVA
IVGA
ASYF
FIL-
-IHIF
IVFS
FFLV
FLIIL
FCN
L--
----R
ALW
MVC
TVLA
VFIIC
FVPH
H----
--Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Mou
se-F
PIVY
SVIF
ILGV
VAN
GYVL
W--
--FM
VNLT
MAD
LLFL
ITLP
LWIV
--N
VAGC
LFFI
NTY
CSVA
FLGV
I---
-GIS
LSLI
IWVS
IVAT
ASYF
LA--
VVHV
FIAF
CFFL
VFFL
IFYC
N---
----R
ALW
MVC
TVLA
VFIIC
FVPH
H----
---I
TLCL
LSTN
CVLD
PVIY
CFLT
--O
poss
um-F
PIVY
SIIF
VLGF
VSN
AYVL
W--
---M
VNLT
VADL
LFLF
ALPL
WIV
Y-N
VAGC
LFFI
NTY
CSVC
FLGV
I---
-GIL
LSTI
IWVV
IVGC
ASYF
LI--
--HVF
IVLC
FFIV
FLVI
LVCN
VI-
------
LWM
VCTV
MAV
FFIC
FVPH
HVV-
---Q
VTLC
LMSI
NCV
LDPI
IYCF
L---
Ora
ngut
an-F
PIVY
SIIF
VLGV
IAN
GYVL
W--
--FM
VNLT
MAD
MLF
LITL
PLW
IV--
NVA
GCLF
FIN
TYCS
VAFL
GVI--
---I
SLSL
VIW
VAIV
GAAS
YFLI
L--IH
IFIV
FSFF
LVFL
IILFC
NL-
----
-RAL
WM
VCTV
LAVF
IICFV
PHH-
----
--VTL
CLLS
TNCV
LDPV
IYCF
LT--
Pig
-FPI
FYSI
IFVL
GVIA
NSY
VLW
-----
MLN
LTM
ADLL
FLVT
LPLW
IIY-
NLA
GCFF
FIN
TYCS
VSFL
AVI--
---I
SLSL
IIWVA
MVA
AASY
FFVL
--IH
IFLV
FSFF
LVFL
VILF
CNL-
----
-RAL
WM
VCTV
LAVF
IICFV
PHH-
----
--ITL
CLLS
TNCV
LDPI
IYCF
LT--
Pika
-FPI
VYSI
IFVL
GVVA
NGY
VLW
----F
MVN
LTVA
DLLF
LITL
PLW
II--
NVA
GCLF
FIN
TYCS
VAFL
GVI--
---I
SLSL
IIWVV
ILAI
ASYF
LIM
--IH
IFIV
FCFF
VVFL
IILVC
NL-
----
-RAL
WM
VCTV
LGVF
IICFV
PHH-
----
-QVT
LCLL
STN
CVLD
PVIY
CFL-
--Pl
atyp
us---
VAYS
IIFVL
GIFA
NGY
VLW
VF--F
MVN
LTVA
DLLF
LVTM
PLW
IV--
NVA
GCLF
FIN
TYCS
VAFL
GVI--
--G
IVLS
LVIW
VLIV
SSAL
YFLV
---IH
IFIV
ASFF
AVFL
LILV
CNL-
----
-RAL
WM
VCTV
LAVF
IICFV
PHH-
----
--VTL
CLLS
TNCV
LDPV
IYCF
LT--
Puffe
rfish
--PSA
YGVI
FILG
LLAN
LYVL
FI-
---M
TNLS
IADL
LFVC
ALPF
WIG
Y----
GSLF
FIN
TYCS
VLFL
GAIS
VN-G
IIISV
IIWVL
TISM
AIPY
LV--
---FA
IIGLF
FVVF
ALVL
VCN
LLI
------
---LL
AVVG
VFVV
CFLP
HHVI
QGA
-QIT
LLLM
GLN
CILD
PVVY
CFA-
--Ra
bbit
--PIV
YSIIF
VLGV
VAN
GYVL
WV-
--FM
VNLT
VADL
LFLI
TLPL
WII-
-N
LAGC
LFFI
NTY
CSVA
FLGV
I---
--IAL
SLVI
WVS
ISAA
ATYF
LIL-
-IHIF
IVAS
FFIV
FLIIL
ICN
L--
----R
ALW
MVC
TVLA
VFIIC
FVPH
H----
--Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Rat
--PIV
YSVI
FVLG
VVAN
GYVL
WV-
--FM
VNLT
VADL
LFLM
TLPL
WIV
--N
LAGC
LFFI
NTY
CSVA
FLGV
I---
-GIT
LSLV
IWIS
IAAT
ASYF
LA--
-VHI
FITS
CFFL
VFFL
IFYC
NM
-----
-RAL
WM
VCTV
LAVF
VICF
VPHH
-----
--ITL
CLLS
TNCV
LDPV
IYCF
LT--
Shre
w-F
PIFY
SIIF
VLGV
IAN
SYVL
W--
--FM
VNLT
MAD
LLFL
ITLP
LWIV
--N
LTGC
FFFI
NTY
CSVA
FLGV
I---
-GIY
LSLV
IWM
AIVS
AASY
FFF-
--IH
IFLV
CSFF
LVFL
LIFF
CNL-
----
-RAL
WM
VCTV
LAVF
SICF
VPHH
-----
--VTL
CLLS
TNCV
LDPI
IYCF
LT--
Squi
rrel
LFPI
IYSI
IFVV
GVIA
NGY
VL---
--FM
LNLT
VADL
LFLI
TLPL
WII-
-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
---YL
SLVI
WVF
IVAA
ASYF
LILD
VIHI
FIVF
SFFL
VFLI
ILFC
K---
----R
ALW
MVC
AVLA
VFVI
CFVP
HH---
---Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Stickleba
ck-F
PVVY
SIIF
ILGI
TAN
LYVL
F--
CIYM
INLT
IGDL
LFVT
FLPF
W---
---S
GSLF
FIN
TYSS
VLFL
CAIS
V--G
IILSV
FIW
LFTL
ATAI
LFLI
----H
LTIV
IGFF
LVFF
VVVV
CNFL
----
----Q
MLF
SVVG
VFVV
CFLP
HHAC
Q--
-QIT
WLL
MSL
NCI
LDPV
VYFF
A---
Zebrafi
sh--P
IFYG
VVFV
LGLI
ANCY
ALYV
--IY
MTN
LTVA
DLLF
VSAL
PFW
I---
---GT
FFFI
NTY
GSIL
FLTV
ISIN
RGAI
ISVL
IWAF
ILSA
SIPQ
L---
ITHF
SIIG
LFFL
VFLM
VIIC
N---
------
LRM
LCAV
IGVF
VICF
LPHH
VV---
-QIT
LMLM
GIN
CLLD
PLVY
CFA-
--
Sequ
ence
Dom
ain
ID
omai
n II
Dom
ain
III
Dom
ain
IVD
omai
n V
Dom
ain
VI
Dom
ain
VII
Tc00
.104
7053
5037
35.3
0-F
KLVV
NCL
MLL
FLIL
IAVM
FH--
PRFF
FSVA
---LL
LLAV
FDIV
LRY
----Y
IAIV
ADTL
GVAY
VFTT
SVRL
LESI
FLGL
TGLL
LSVN
LLSY
I----
-LGF
AMSV
APIF
FGFA
LFFC
IV--
MGF
SLVA
LYFV
MFG
DAIL
PAI--
---LI
VTVL
ANFM
TMLF
ILFF
MM
T---
Hum
an-F
PIVY
SIIF
VLGV
IAN
GYVL
W--
--FM
VNLT
MAD
MLF
LITL
PLW
IV-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
-----
ISLS
LVIW
VAIV
GAAS
YFLI
L--IH
IFIV
FSFF
LVFL
IILFC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
-VTL
CLLS
TNCV
LDPV
IYCF
LT--
Chic
ken
--TVF
YSIIF
ILGF
VAN
CYVL
WI-
--FM
LNLT
VADL
LFLV
TMPL
WIV
-SV
AGSL
FFVN
TYAS
VAFL
MVI
----
--GIF
LSAA
IWIIT
VGSA
LYYI
F--
AIHI
IICTL
FYVI
FLFI
LTW
N---
RALW
MVC
TVLV
VFIIS
FVPH
H----
--V
TLCL
LSM
NCV
LDPV
IYCF
LT--
Chim
panz
ee-F
PIVY
SIIF
VLGV
IAN
GYVL
W--
--FM
VNLT
MAD
MLF
LITL
PLW
IV-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
-----
ISLS
LVIW
VAIV
GAAS
YFLI
L--IH
IFIV
FSFF
LVFL
IILFC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
-VTL
CLLS
TNCV
LDPV
IYCF
LT--
Cow
-FPI
FYSI
VFVL
GVIA
NSY
VLW
-----
MVN
LTM
ADLL
FLVT
LPLW
IVY
NLA
GCFF
FIN
TYCS
VAFL
AVI--
-----
ILLS
LIIW
VSIV
GAAS
YFFV
L--IH
IFLV
FSFF
LVFL
IILFC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
-VTL
CLLS
TNCV
LDPI
IYCF
LT--
Elep
hant
-FPI
FYSI
IFVL
GVIA
NGY
VLW
-----
MVN
LTM
ADLL
FLVT
LPLW
IVY
NLA
GCLF
FIN
TYCS
VAFL
GVI--
-----
ITLS
LVIW
VTIV
AAAS
YFLF
M-
--HIF
IVCS
FFLV
FFIIL
VCN
LV-
RALW
MVC
TVLA
VFIIC
FVPH
H----
-Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Frog
---VT
YSIIF
VFGF
IAN
CYVL
WIF
-IFM
INLT
IADL
LFLV
TLPL
WI--
NVA
GCFF
FLN
TYCS
VAFL
GVI--
-----
-CIS
VAVW
ILLF
ASSL
YFLI
FP--H
FILV
GAFF
VVFL
IILTC
NLV
-RA
LWM
VVTV
LAVF
VLCF
VPHH
-----
---LC
LMST
NCM
LDPI
IYCF
LTKK
Goat
-FPI
FYSI
VFVL
GVIA
NSY
VLW
-----
MVN
LTM
ADLL
FLVT
LPLW
IVY
NLA
GCFF
FIN
TYCS
VAFL
AVI--
-----
FLLS
LIIW
VSIV
GAAS
YFFV
L--IH
IFLV
FSFF
LVFL
IILFC
NL-
-RA
LWM
VCTV
LAVF
VICF
VPHH
-----
-VTL
CLLS
TNCV
LDPI
IYCF
LT--
Guin
ea p
ig-F
PIVY
SIIF
VLGI
IAN
GYVL
W--
--FM
VNLT
VADL
LFLI
TLPL
WIV
-N
LAGC
LFFI
NTY
CSVA
FLGV
I----
---IA
LSLV
IWVA
IVAA
ASYF
LVM
--IH
ICIV
LGFF
IVFL
LILF
CNL-
-RA
LWM
VCTV
LAVF
VICF
VPHH
-----
QVT
LCLL
STN
CVLD
PVIY
CFL-
--He
dgeh
og-F
PIFY
SIIF
ALGV
IAN
SYVL
W--
--FM
VNLT
LADL
LFLV
TLPL
WII-
NLA
GCFF
FIN
TYCS
VAFL
GVI--
----G
IILSL
VIW
VAIV
SAAS
YFFF
---IH
IFLV
VSFF
VVFL
IIFFC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
QIT
LCLL
STN
CVLD
PIIY
CFL-
--Ho
rse
--PIV
YSIIF
VLGV
IAN
SYVL
WV-
NLT
GCCF
FIN
TYCS
VAFL
GVI--
---IF
MVN
LTVA
DLLF
LLTL
PLW
V--
---IS
LSLV
IWLA
IVAA
ASYF
LFM
--IH
IFLV
FSFF
LVFL
IILVC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
QVT
LCLL
STN
CVLD
PIIY
CFL-
--M
edak
a-F
SVVY
SIFF
VVGL
LAN
VYVL
F--
--YM
TNLT
IADL
LFVC
ALPF
WIG
---T
GSFF
FIN
TYGT
ILFL
GAIS
L--
-RGI
IVSV
LIW
LCLV
SSAI
PSL-
----H
FLIIG
LFFV
VFFL
VMVC
NLL
----
QM
LLAV
VGVF
LLCF
LPHH
VVQ
--Q
ITLV
LMGL
NCI
LDPV
IYYF
A---
Mon
key
-FPI
VYSI
IFVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
-N
LAGC
LFFI
NTY
CSVA
FLGV
I----
---IS
LSLV
IWVA
IVGA
ASYF
FIL-
-IHIF
IVFS
FFLV
FLIIL
FCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
-Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Mou
se-F
PIVY
SVIF
ILGV
VAN
GYVL
W--
--FM
VNLT
MAD
LLFL
ITLP
LWIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I----
--GIS
LSLI
IWVS
IVAT
ASYF
LA--
VVHV
FIAF
CFFL
VFFL
IFYC
N---
RALW
MVC
TVLA
VFIIC
FVPH
H----
--IT
LCLL
STN
CVLD
PVIY
CFLT
--O
poss
um-F
PIVY
SIIF
VLGF
VSN
AYVL
W--
---M
VNLT
VADL
LFLF
ALPL
WIV
YN
VAGC
LFFI
NTY
CSVC
FLGV
I----
--GIL
LSTI
IWVV
IVGC
ASYF
LI--
--HVF
IVLC
FFIV
FLVI
LVCN
VI-
--LW
MVC
TVM
AVFF
ICFV
PHHV
V---
QVT
LCLM
SIN
CVLD
PIIY
CFL-
--O
rang
utan
-FPI
VYSI
IFVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I----
---IS
LSLV
IWVA
IVGA
ASYF
LIL-
-IHIF
IVFS
FFLV
FLIIL
FCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
--V
TLCL
LSTN
CVLD
PVIY
CFLT
--Pi
g-F
PIFY
SIIF
VLGV
IAN
SYVL
W--
---M
LNLT
MAD
LLFL
VTLP
LWIIY
NLA
GCFF
FIN
TYCS
VSFL
AVI--
-----
ISLS
LIIW
VAM
VAAA
SYFF
VL-
-IHIF
LVFS
FFLV
FLVI
LFCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
--IT
LCLL
STN
CVLD
PIIY
CFLT
--Pi
ka-F
PIVY
SIIF
VLGV
VAN
GYVL
W--
--FM
VNLT
VADL
LFLI
TLPL
WII-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
-----
ISLS
LIIW
VVIL
AIAS
YFLI
M-
-IHIF
IVFC
FFVV
FLIIL
VCN
L--
QVT
LCLL
STN
CVLD
PVIY
CFL-
--RA
LWM
VCTV
LGVF
IICFV
PHH-
----
Plat
ypus
---VA
YSIIF
VLGI
FAN
GYVL
WVF
--FM
VNLT
VADL
LFLV
TMPL
WIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I----
-IHIF
IVAS
FFAV
FLLI
LVCN
L--
--GIV
LSLV
IWVL
IVSS
ALYF
LV--
RALW
MVC
TVLA
VFIIC
FVPH
H----
--V
TLCL
LSTN
CVLD
PVIY
CFLT
--Pu
fferfish
--PSA
YGVI
FILG
LLAN
LYVL
FI-
---M
TNLS
IADL
LFVC
ALPF
WIG
Y---
GSLF
FIN
TYCS
VLFL
GAIS
VN-
--GIII
SVIIW
VLTI
SMAI
PYLV
-----
FAIIG
LFFV
VFAL
VLVC
NLL
I---
--LLA
VVGV
FVVC
FLPH
HVIQ
GAQ
ITLL
LMGL
NCI
LDPV
VYCF
A---
Rabb
it--P
IVYS
IIFVL
GVVA
NGY
VLW
V---F
MVN
LTVA
DLLF
LITL
PLW
II-N
LAGC
LFFI
NTY
CSVA
FLGV
I----
---IA
LSLV
IWVS
ISAA
ATYF
LIL-
-IHIF
IVAS
FFIV
FLIIL
ICN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
-Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Rat
--PIV
YSVI
FVLG
VVAN
GYVL
WV-
--FM
VNLT
VADL
LFLM
TLPL
WIV
-N
LAGC
LFFI
NTY
CSVA
FLGV
I----
--GIT
LSLV
IWIS
IAAT
ASYF
LA--
RALW
MVC
TVLA
VFVI
CFVP
HH---
---V
HIFI
TSCF
FLVF
FLIF
YCN
M--
-ITLC
LLST
NCV
LDPV
IYCF
LT--
Shre
w-F
PIFY
SIIF
VLGV
IAN
SYVL
W--
--FM
VNLT
MAD
LLFL
ITLP
LWIV
-N
LTGC
FFFI
NTY
CSVA
FLGV
I----
--GIY
LSLV
IWM
AIVS
AASY
FFF-
--IH
IFLV
CSFF
LVFL
LIFF
CNL-
-RA
LWM
VCTV
LAVF
SICF
VPHH
-----
-VTL
CLLS
TNCV
LDPI
IYCF
LT--
Squi
rrel
LFPI
IYSI
IFVV
GVIA
NGY
VL---
--FM
LNLT
VADL
LFLI
TLPL
WII-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
-----
-YLS
LVIW
VFIV
AAAS
YFLI
LDVI
HIFI
VFSF
FLVF
LIIL
FCK-
--RA
LWM
VCAV
LAVF
VICF
VPHH
-----
QVT
LCLL
STN
CVLD
PVIY
CFL-
--Sti
ckleba
ck-F
PVVY
SIIF
ILGI
TAN
LYVL
F--
CIYM
INLT
IGDL
LFVT
FLPF
W---
--SGS
LFFI
NTY
SSVL
FLCA
ISV-
---G
IILSV
FIW
LFTL
ATAI
LFLI
----H
LTIV
IGFF
LVFF
VVVV
CNFL
----
QM
LFSV
VGVF
VVCF
LPHH
ACQ
--Q
ITW
LLM
SLN
CILD
PVVY
FFA-
--Zebrafi
sh-IY
MTN
LTVA
DLLF
VSAL
PFW
I----P
IFYG
VVFV
LGLI
ANCY
ALYV
----
GTFF
FIN
TYGS
ILFL
TVIS
IN-
-RGA
IISVL
IWAF
ILSA
SIPQ
L---
--LRM
LCAV
IGVF
VICF
LPHH
VV---
ITHF
SIIG
LFFL
VFLM
VIIC
N---
QIT
LMLM
GIN
CLLD
PLVY
CFA-
--
11Supplementary data Search for PAF receptor in T. cruzi • Daniel Fábio Kawano et al.
Sequ
ence
Dom
ain
ID
omai
n II
Dom
ain
III
Dom
ain
IVD
omai
n V
Dom
ain
VI
Dom
ain
VII
Tc00
.104
7053
5103
55.1
90-L
EMVF
FIN
IVGC
LVPG
GACL
L--
KLFF
VVN
FVN
VLAT
YLM
FSSF
----
------
VSSS
TWVS
VFFV
CIGA
SLTS
G-S
LGAA
VIM
FTYI
LVFA
SNAA
L--
CLVH
FFVL
MLS
SMTL
LGPV
VA---
----
--QIV
VVGV
SYGV
YQVF
NGV
VLF
DSVL
QLL
LGLI
LLFV
GSYC
YV---
Hum
an-F
PIVY
SIIF
VLGV
IAN
GYVL
W--
---FM
VNLT
MAD
MLF
LITL
PLW
IV-
NVA
GCLF
FIN
TYCS
VAFL
GV---
I---
--ISL
SLVI
WVA
IVGA
ASYF
LIL-
--IHI
FIVF
SFFL
VFLI
ILFC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
-VTL
CLLS
TNCV
LDPV
IYCF
LT--
Chic
ken
--TVF
YSIIF
ILGF
VAN
CYVL
WI-
---FM
LNLT
VADL
LFLV
TMPL
WIV
-SV
AGSL
FFVN
TYAS
VAFL
MV-
--I---
-GIF
LSAA
IWIIT
VGSA
LYYI
F--
-AIH
IIICT
LFYV
IFLF
ILTW
N---
RALW
MVC
TVLV
VFIIS
FVPH
H----
--V
TLCL
LSM
NCV
LDPV
IYCF
LT--
Chim
panz
ee-F
PIVY
SIIF
VLGV
IAN
GYVL
W--
---FM
VNLT
MAD
MLF
LITL
PLW
IV-
NVA
GCLF
FIN
TYCS
VAFL
GV---
I---
--ISL
SLVI
WVA
IVGA
ASYF
LIL-
--IHI
FIVF
SFFL
VFLI
ILFC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
-VTL
CLLS
TNCV
LDPV
IYCF
LT--
Cow
-FPI
FYSI
VFVL
GVIA
NSY
VLW
-----
-MVN
LTM
ADLL
FLVT
LPLW
IVY
NLA
GCFF
FIN
TYCS
VAFL
AV---
I---
--ILL
SLIIW
VSIV
GAAS
YFFV
L---I
HIFL
VFSF
FLVF
LIIL
FCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
--V
TLCL
LSTN
CVLD
PIIY
CFLT
--El
epha
nt-F
PIFY
SIIF
VLGV
IAN
GYVL
W--
----M
VNLT
MAD
LLFL
VTLP
LWIV
YN
LAGC
LFFI
NTY
CSVA
FLGV
---I--
---I
TLSL
VIW
VTIV
AAAS
YFLF
M-
---HI
FIVC
SFFL
VFFI
ILVCN
LV-
RALW
MVC
TVLA
VFIIC
FVPH
H----
-Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Frog
---VT
YSIIF
VFGF
IAN
CYVL
WIF
--IFM
INLT
IADL
LFLV
TLPL
WI--
NVA
GCFF
FLN
TYCS
VAFL
GV---
I---
---CI
SVAV
WIL
LFAS
SLYF
LIFP
---HF
ILVGA
FFVV
FLIIL
TCN
LV-
RALW
MVV
TVLA
VFVL
CFVP
HH---
-----
LCLM
STN
CMLD
PIIY
CFLT
KKGo
at-F
PIFY
SIVF
VLGV
IAN
SYVL
W--
----M
VNLT
MAD
LLFL
VTLP
LWIV
YN
LAGC
FFFI
NTY
CSVA
FLAV
---I--
---F
LLSL
IIWVS
IVGA
ASYF
FVL-
--IHI
FLVF
SFFL
VFLI
ILFC
NL-
-RA
LWM
VCTV
LAVF
VICF
VPHH
-----
-VTL
CLLS
TNCV
LDPI
IYCF
LT--
Guin
ea p
ig-F
PIVY
SIIF
VLGI
IAN
GYVL
W--
---FM
VNLT
VADL
LFLI
TLPL
WIV
-N
LAGC
LFFI
NTY
CSVA
FLGV
---I--
---I
ALSL
VIW
VAIV
AAAS
YFLV
M-
--IHI
CIVL
GFFI
VFLL
ILFC
NL-
-RA
LWM
VCTV
LAVF
VICF
VPHH
-----
QVT
LCLL
STN
CVLD
PVIY
CFL-
--He
dgeh
og-F
PIFY
SIIF
ALGV
IAN
SYVL
W--
---FM
VNLT
LADL
LFLV
TLPL
WII-
NLA
GCFF
FIN
TYCS
VAFL
GV---
I---
-GIIL
SLVI
WVA
IVSA
ASYF
FF--
--IHI
FLVV
SFFV
VFLI
IFFC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
QIT
LCLL
STN
CVLD
PIIY
CFL-
--Ho
rse
--PIV
YSIIF
VLGV
IAN
SYVL
WV-
--IFM
VNLT
VADL
LFLL
TLPL
WV-
-N
LTGC
CFFI
NTY
CSVA
FLGV
---I--
---I
SLSL
VIW
LAIV
AAAS
YFLF
M-
--IHI
FLVF
SFFL
VFLI
ILVCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
-Q
VTLC
LLST
NCV
LDPI
IYCF
L---
Med
aka
-FSV
VYSI
FFVV
GLLA
NVY
VLF-
----
YMTN
LTIA
DLLF
VCAL
PFW
IG-
--TGS
FFFI
NTY
GTIL
FLGA
---IS
L-RG
IIVSV
LIW
LCLV
SSAI
PSL-
-----
HFLI
IGLF
FVVF
FLVM
VCN
LL-
---Q
MLL
AVVG
VFLL
CFLP
HHVV
Q--
QIT
LVLM
GLN
CILD
PVIY
YFA-
--M
onke
y-F
PIVY
SIIF
VLGV
IAN
GYVL
W--
---FM
VNLT
MAD
MLF
LITL
PLW
IV-
NLA
GCLF
FIN
TYCS
VAFL
GV---
I---
--ISL
SLVI
WVA
IVGA
ASYF
FIL-
--IHI
FIVF
SFFL
VFLI
ILFC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
QVT
LCLL
STN
CVLD
PVIY
CFL-
--M
ouse
-FPI
VYSV
IFIL
GVVA
NGY
VLW
-----
FMVN
LTM
ADLL
FLIT
LPLW
IV-
NVA
GCLF
FIN
TYCS
VAFL
GV---
I---
-GIS
LSLI
IWVS
IVAT
ASYF
LA--
-VVH
VFIA
FCFF
LVFF
LIFY
CN---
RALW
MVC
TVLA
VFIIC
FVPH
H----
--IT
LCLL
STN
CVLD
PVIY
CFLT
--O
poss
um-F
PIVY
SIIF
VLGF
VSN
AYVL
W--
----M
VNLT
VADL
LFLF
ALPL
WIV
YN
VAGC
LFFI
NTY
CSVC
FLGV
---I--
--G
ILLS
TIIW
VVIV
GCAS
YFLI
-----
HVFI
VLCF
FIVF
LVILV
CNVI
---L
WM
VCTV
MAV
FFIC
FVPH
HVV-
--Q
VTLC
LMSI
NCV
LDPI
IYCF
L---
Ora
ngut
an-F
PIVY
SIIF
VLGV
IAN
GYVL
W--
---FM
VNLT
MAD
MLF
LITL
PLW
IV-
NVA
GCLF
FIN
TYCS
VAFL
GV---
I---
--ISL
SLVI
WVA
IVGA
ASYF
LIL-
--IHI
FIVF
SFFL
VFLI
ILFC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
-VTL
CLLS
TNCV
LDPV
IYCF
LT--
Pig
-FPI
FYSI
IFVL
GVIA
NSY
VLW
-----
-MLN
LTM
ADLL
FLVT
LPLW
IIYN
LAGC
FFFI
NTY
CSVS
FLAV
---I--
---I
SLSL
IIWVA
MVA
AASY
FFVL
---I
HIFL
VFSF
FLVF
LVIL
FCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
--IT
LCLL
STN
CVLD
PIIY
CFLT
--Pi
ka-F
PIVY
SIIF
VLGV
VAN
GYVL
W--
---FM
VNLT
VADL
LFLI
TLPL
WII-
NVA
GCLF
FIN
TYCS
VAFL
GV---
I---
--ISL
SLIIW
VVIL
AIAS
YFLI
M-
--IHI
FIVF
CFFV
VFLI
ILVCN
L--
RALW
MVC
TVLG
VFIIC
FVPH
H----
-Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Plat
ypus
---VA
YSIIF
VLGI
FAN
GYVL
WVF
---FM
VNLT
VADL
LFLV
TMPL
WIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
---I--
--G
IVLS
LVIW
VLIV
SSAL
YFLV
----I
HIFI
VASF
FAVF
LLILV
CNL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
-VTL
CLLS
TNCV
LDPV
IYCF
LT--
Puffe
rfish
--PSA
YGVI
FILG
LLAN
LYVL
FI-
----M
TNLS
IADL
LFVC
ALPF
WIG
Y---
GSLF
FIN
TYCS
VLFL
GA---
ISVN
-GIII
SVIIW
VLTI
SMAI
PYLV
-----
-FAI
IGLF
FVVF
ALVL
VCN
LLI
-----L
LAVV
GVFV
VCFL
PHHV
IQGA
QIT
LLLM
GLN
CILD
PVVY
CFA-
--Ra
bbit
--PIV
YSIIF
VLGV
VAN
GYVL
WV-
---FM
VNLT
VADL
LFLI
TLPL
WII-
NLA
GCLF
FIN
TYCS
VAFL
GV---
I---
--IAL
SLVI
WVS
ISAA
ATYF
LIL-
--IHI
FIVA
SFFI
VFLI
ILIC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
QVT
LCLL
STN
CVLD
PVIY
CFL-
--Ra
t--P
IVYS
VIFV
LGVV
ANGY
VLW
V----
FMVN
LTVA
DLLF
LMTL
PLW
IV-
NLA
GCLF
FIN
TYCS
VAFL
GV---
I---
-GIT
LSLV
IWIS
IAAT
ASYF
LA--
--VHI
FITS
CFFL
VFFL
IFYC
NM
--RA
LWM
VCTV
LAVF
VICF
VPHH
-----
-ITLC
LLST
NCV
LDPV
IYCF
LT--
Shre
w-F
PIFY
SIIF
VLGV
IAN
SYVL
W--
---FM
VNLT
MAD
LLFL
ITLP
LWIV
-N
LTGC
FFFI
NTY
CSVA
FLGV
---I--
--G
IYLS
LVIW
MAI
VSAA
SYFF
F--
--IHI
FLVC
SFFL
VFLL
IFFC
NL-
-RA
LWM
VCTV
LAVF
SICF
VPHH
-----
-VTL
CLLS
TNCV
LDPI
IYCF
LT--
Squi
rrel
LFPI
IYSI
IFVV
GVIA
NGY
VL---
---FM
LNLT
VADL
LFLI
TLPL
WII-
NVA
GCLF
FIN
TYCS
VAFL
GV---
I---
---YL
SLVI
WVF
IVAA
ASYF
LILD
-VIH
IFIV
FSFF
LVFL
IILFC
K---
RALW
MVC
AVLA
VFVI
CFVP
HH---
--Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Stickleba
ck-F
PVVY
SIIF
ILGI
TAN
LYVL
F--
-CIY
MIN
LTIG
DLLF
VTFL
PFW
-----S
GSLF
FIN
TYSS
VLFL
CA---
ISV-
-GIIL
SVFI
WLF
TLAT
AILF
LI--
---HL
TIVI
GFFL
VFFV
VVVC
NFL
----
QM
LFSV
VGVF
VVCF
LPHH
ACQ
--Q
ITW
LLM
SLN
CILD
PVVY
FFA-
--Zebrafi
sh--P
IFYG
VVFV
LGLI
ANCY
ALYV
---I
YMTN
LTVA
DLLF
VSAL
PFW
I-----
GTFF
FIN
TYGS
ILFL
TV---
ISIN
RGAI
ISVL
IWAF
ILSA
SIPQ
L---
-ITHF
SIIG
LFFL
VFLM
VIIC
N---
--LRM
LCAV
IGVF
VICF
LPHH
VV---
QIT
LMLM
GIN
CLLD
PLVY
CFA-
--
Sequ
ence
Dom
ain
ID
omai
n II
Dom
ain
III
Dom
ain
IVD
omai
n V
Dom
ain
VI
Dom
ain
VII
Tc00
.104
7053
5097
19.5
6-L
SFSL
SLSL
FFFF
LHVC
TLPL
--TR
YLFW
LGLI
VLLW
TFTA
GRE-
-----
GQLF
-LAV
LCVL
VTM
CFILV
TKGP
LFLI
FLLW
IGG-
----Y
YLKF
CMY
---HW
VLGI
YSVV
ALCL
NLW
ALEY
LLLL
IISL-
----C
TVEI
PLN
YVG
YGLG
IIVTA
VSSI
TLLP
HLYV
-----
Hum
an-F
PIVY
SIIF
VLGV
IAN
GYVL
W--
--FM
VNLT
MAD
MLF
LITL
PLW
IV-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
----I
SLSL
VIW
VAIV
GAAS
YFLI
L---
-IHIF
IVFS
FFLV
FLIIL
FCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
---V
TLCL
LSTN
CV-L
DPVI
YCFL
T--
Chic
ken
--TVF
YSIIF
ILGF
VAN
CYVL
WI-
--FM
LNLT
VADL
LFLV
TMPL
WIV
-SV
AGSL
FFVN
TYAS
VAFL
MVI
----
-GIF
LSAA
IWIIT
VGSA
LYYI
F----
AIHI
IICTL
FYVI
FLFI
LTW
N---
RALW
MVC
TVLV
VFIIS
FVPH
H----
---V
TLCL
LSM
NCV
-LDP
VIYC
FLT-
-Ch
impa
nzee
-FPI
VYSI
IFVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I----
--ISL
SLVI
WVA
IVGA
ASYF
LIL-
---IH
IFIV
FSFF
LVFL
IILFC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
--VTL
CLLS
TNCV
-LDP
VIYC
FLT-
-Co
w-F
PIFY
SIVF
VLGV
IAN
SYVL
W--
NLA
GCFF
FIN
TYCS
VAFL
AVI--
-----
MVN
LTM
ADLL
FLVT
LPLW
IVY
--ILL
SLIIW
VSIV
GAAS
YFFV
L---
-IHIF
LVFS
FFLV
FLIIL
FCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
---V
TLCL
LSTN
CV-L
DPIIY
CFLT
--El
epha
nt-F
PIFY
SIIF
VLGV
IAN
GYVL
W--
---M
VNLT
MAD
LLFL
VTLP
LWIV
YN
LAGC
LFFI
NTY
CSVA
FLGV
I----
--ITL
SLVI
WVT
IVAA
ASYF
LFM
-----H
IFIV
CSFF
LVFF
IILVC
NLV
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
-QVT
LCLL
STN
CV-L
DPVI
YCFL
---Fr
og---
VTYS
IIFVF
GFIA
NCY
VLW
IF-IF
MIN
LTIA
DLLF
LVTL
PLW
I--N
VAGC
FFFL
NTY
CSVA
FLGV
I----
---CI
SVAV
WIL
LFAS
SLYF
LIFP
----H
FILV
GAFF
VVFL
IILTC
NLV
-RA
LWM
VVTV
LAVF
VLCF
VPHH
-----
----L
CLM
STN
CM-L
DPIIY
CFLT
KKGo
at-F
PIFY
SIVF
VLGV
IAN
SYVL
W--
---M
VNLT
MAD
LLFL
VTLP
LWIV
YN
LAGC
FFFI
NTY
CSVA
FLAV
I----
--FLL
SLIIW
VSIV
GAAS
YFFV
L---
-IHIF
LVFS
FFLV
FLIIL
FCN
L--
RALW
MVC
TVLA
VFVI
CFVP
HH---
----V
TLCL
LSTN
CV-L
DPIIY
CFLT
--Gu
inea
pig
-FPI
VYSI
IFVL
GIIA
NGY
VLW
----F
MVN
LTVA
DLLF
LITL
PLW
IV-
NLA
GCLF
FIN
TYCS
VAFL
GVI--
----I
ALSL
VIW
VAIV
AAAS
YFLV
M---
-IHIC
IVLG
FFIV
FLLI
LFCN
L--
RALW
MVC
TVLA
VFVI
CFVP
HH---
---Q
VTLC
LLST
NCV
-LDP
VIYC
FL---
Hedg
ehog
-FPI
FYSI
IFAL
GVIA
NSY
VLW
----F
MVN
LTLA
DLLF
LVTL
PLW
II-N
LAGC
FFFI
NTY
CSVA
FLGV
I----
-GIIL
SLVI
WVA
IVSA
ASYF
FF---
--IH
IFLV
VSFF
VVFL
IIFFC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
-QIT
LCLL
STN
CV-L
DPIIY
CFL-
--Ho
rse
--PIV
YSIIF
VLGV
IAN
SYVL
WV-
-IFM
VNLT
VADL
LFLL
TLPL
WV-
-N
LTGC
CFFI
NTY
CSVA
FLGV
I----
--ISL
SLVI
WLA
IVAA
ASYF
LFM
----IH
IFLV
FSFF
LVFL
IILVC
NL-
--Q
VTLC
LLST
NCV
-LDP
IIYCF
L---
RALW
MVC
TVLA
VFIIC
FVPH
H----
-M
edak
a-F
SVVY
SIFF
VVGL
LAN
VYVL
F--
--YM
TNLT
IADL
LFVC
ALPF
WIG
---T
GSFF
FIN
TYGT
ILFL
GAIS
L--
RGIIV
SVLI
WLC
LVSS
AIPS
L----
---H
FLIIG
LFFV
VFFL
VMVC
NLL
----
QM
LLAV
VGVF
LLCF
LPHH
VVQ
---Q
ITLV
LMGL
NCI
-LDP
VIYY
FA---
Mon
key
-FPI
VYSI
IFVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
-N
LAGC
LFFI
NTY
CSVA
FLGV
I----
--ISL
SLVI
WVA
IVGA
ASYF
FIL-
---IH
IFIV
FSFF
LVFL
IILFC
NL-
--Q
VTLC
LLST
NCV
-LDP
VIYC
FL---
RALW
MVC
TVLA
VFIIC
FVPH
H----
-M
ouse
-FPI
VYSV
IFIL
GVVA
NGY
VLW
----F
MVN
LTM
ADLL
FLIT
LPLW
IV-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
---G
ISLS
LIIW
VSIV
ATAS
YFLA
----
VVHV
FIAF
CFFL
VFFL
IFYC
N---
RALW
MVC
TVLA
VFIIC
FVPH
H----
---I
TLCL
LSTN
CV-L
DPVI
YCFL
T--
Opo
ssum
-FPI
VYSI
IFVL
GFVS
NAY
VLW
-----
MVN
LTVA
DLLF
LFAL
PLW
IVY
NVA
GCLF
FIN
TYCS
VCFL
GVI--
----H
VFIV
LCFF
IVFL
VILV
CNVI
--G
ILLS
TIIW
VVIV
GCAS
YFLI
----
--LW
MVC
TVM
AVFF
ICFV
PHHV
V---
-QVT
LCLM
SIN
CV-L
DPIIY
CFL-
--O
rang
utan
-FPI
VYSI
IFVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I----
--ISL
SLVI
WVA
IVGA
ASYF
LIL-
---IH
IFIV
FSFF
LVFL
IILFC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
--VTL
CLLS
TNCV
-LDP
VIYC
FLT-
-Pi
g-F
PIFY
SIIF
VLGV
IAN
SYVL
W--
---M
LNLT
MAD
LLFL
VTLP
LWIIY
NLA
GCFF
FIN
TYCS
VSFL
AVI--
----I
SLSL
IIWVA
MVA
AASY
FFVL
----IH
IFLV
FSFF
LVFL
VILF
CNL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
--ITL
CLLS
TNCV
-LDP
IIYCF
LT--
Pika
--FM
VNLT
VADL
LFLI
TLPL
WII-
-FPI
VYSI
IFVL
GVVA
NGY
VLW
--N
VAGC
LFFI
NTY
CSVA
FLGV
I----
--ISL
SLIIW
VVIL
AIAS
YFLI
M---
-IHIF
IVFC
FFVV
FLIIL
VCN
L--
RALW
MVC
TVLG
VFIIC
FVPH
H----
--Q
VTLC
LLST
NCV
-LDP
VIYC
FL---
Plat
ypus
---VA
YSIIF
VLGI
FAN
GYVL
WVF
--FM
VNLT
VADL
LFLV
TMPL
WIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I----
-GIV
LSLV
IWVL
IVSS
ALYF
LV---
--IH
IFIV
ASFF
AVFL
LILV
CNL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
--VTL
CLLS
TNCV
-LDP
VIYC
FLT-
-Pu
fferfish
--PSA
YGVI
FILG
LLAN
LYVL
FI-
---M
TNLS
IADL
LFVC
ALPF
WIG
Y---
GSLF
FIN
TYCS
VLFL
GAIS
VN-
-GIII
SVIIW
VLTI
SMAI
PYLV
----
---FA
IIGLF
FVVF
ALVL
VCN
LLI
-----L
LAVV
GVFV
VCFL
PHHV
IQGA
-QIT
LLLM
GLN
CI-L
DPVV
YCFA
---Ra
bbit
--PIV
YSIIF
VLGV
VAN
GYVL
WV-
--FM
VNLT
VADL
LFLI
TLPL
WII-
NLA
GCLF
FIN
TYCS
VAFL
GVI--
----I
ALSL
VIW
VSIS
AAAT
YFLI
L---
-IHIF
IVAS
FFIV
FLIIL
ICN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
--Q
VTLC
LLST
NCV
-LDP
VIYC
FL---
Rat
--PIV
YSVI
FVLG
VVAN
GYVL
WV-
--FM
VNLT
VADL
LFLM
TLPL
WIV
-N
LAGC
LFFI
NTY
CSVA
FLGV
I----
-GIT
LSLV
IWIS
IAAT
ASYF
LA---
--V
HIFI
TSCF
FLVF
FLIF
YCN
M--
RALW
MVC
TVLA
VFVI
CFVP
HH---
----I
TLCL
LSTN
CV-L
DPVI
YCFL
T--
Shre
w-F
PIFY
SIIF
VLGV
IAN
SYVL
W--
--FM
VNLT
MAD
LLFL
ITLP
LWIV
-N
LTGC
FFFI
NTY
CSVA
FLGV
I----
-GIY
LSLV
IWM
AIVS
AASY
FFF-
----IH
IFLV
CSFF
LVFL
LIFF
CNL-
-RA
LWM
VCTV
LAVF
SICF
VPHH
-----
--VTL
CLLS
TNCV
-LDP
IIYCF
LT--
Squi
rrel
LFPI
IYSI
IFVV
GVIA
NGY
VL---
--FM
LNLT
VADL
LFLI
TLPL
WII-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
-----
YLSL
VIW
VFIV
AAAS
YFLI
LD--
VIHI
FIVF
SFFL
VFLI
ILFC
K---
RALW
MVC
AVLA
VFVI
CFVP
HH---
---Q
VTLC
LLST
NCV
-LDP
VIYC
FL---
Stickleba
ck-F
PVVY
SIIF
ILGI
TAN
LYVL
F--
CIYM
INLT
IGDL
LFVT
FLPF
W---
--SGS
LFFI
NTY
SSVL
FLCA
ISV-
--G
IILSV
FIW
LFTL
ATAI
LFLI
----
--HLT
IVIG
FFLV
FFVV
VVCN
FL-
---Q
MLF
SVVG
VFVV
CFLP
HHAC
Q--
-QIT
WLL
MSL
NCI
-LDP
VVYF
FA---
Zebrafi
sh--P
IFYG
VVFV
LGLI
ANCY
ALYV
--IY
MTN
LTVA
DLLF
VSAL
PFW
I-----
GTFF
FIN
TYGS
ILFL
TVIS
IN-
RGAI
ISVL
IWAF
ILSA
SIPQ
L----
-IT
HFSI
IGLF
FLVF
LMVI
ICN
-----L
RMLC
AVIG
VFVI
CFLP
HHVV
----Q
ITLM
LMGI
NCL
-LDP
LVYC
FA---
12Supplementary data Search for PAF receptor in T. cruzi • Daniel Fábio Kawano et al.
Sequ
ence
Dom
ain
ID
omai
n II
Dom
ain
III
Dom
ain
IVD
omai
n V
Dom
ain
VI
Dom
ain
VII
Tc00
.104
7053
5083
17.7
0---
SFFI
SFFF
FFFD
STGE
IEGF
V-F
FTLV
LLFC
SWGM
LLQ
MAM
VI--
EFSS
VALG
IAAY
-AVL
FLSE
SS--
KFFL
IPLM
VILA
LHFL
V-HL
MI--
------
-LSR
FLAD
FIM
FFCG
CAVL
LGL
--IAM
TCGC
LMIM
FFLL
IAPS
FF---
KNGV
IPPA
SVLV
LVLD
AVSR
Q---
------
Hum
an-F
PIVY
SIIF
VLGV
IAN
GYVL
W--
--FM
VNLT
MAD
MLF
LITL
PLW
IV-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
---I
SLSL
VIW
VAIV
GAAS
YFLI
L--IH
IFIV
FSFF
LVFL
IILFC
NL-
-----
RALW
MVC
TVLA
VFIIC
FVPH
H----
----
----V
TLCL
LSTN
CVLD
PVIY
CFLT
--Ch
icke
n--T
VFYS
IIFIL
GFVA
NCY
VLW
I---F
MLN
LTVA
DLLF
LVTM
PLW
IV-
SVAG
SLFF
VNTY
ASVA
FLM
VI---
-GIF
LSAA
IWIIT
VGSA
LYYI
F--
RALW
MVC
TVLV
VFIIS
FVPH
H----
-AI
HIIIC
TLFY
VIFL
FILT
WN
------
----
----V
TLCL
LSM
NCV
LDPV
IYCF
LT--
Chim
panz
ee-F
PIVY
SIIF
VLGV
IAN
GYVL
W--
--FM
VNLT
MAD
MLF
LITL
PLW
IV-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
---I
SLSL
VIW
VAIV
GAAS
YFLI
L--IH
IFIV
FSFF
LVFL
IILFC
NL-
-----
RALW
MVC
TVLA
VFIIC
FVPH
H----
----
----V
TLCL
LSTN
CVLD
PVIY
CFLT
--Co
w-F
PIFY
SIVF
VLGV
IAN
SYVL
W--
---M
VNLT
MAD
LLFL
VTLP
LWIV
YN
LAGC
FFFI
NTY
CSVA
FLAV
I---
--ILL
SLIIW
VSIV
GAAS
YFFV
L--IH
IFLV
FSFF
LVFL
IILFC
NL-
-----
RALW
MVC
TVLA
VFIIC
FVPH
H----
----
----V
TLCL
LSTN
CVLD
PIIY
CFLT
--El
epha
nt-F
PIFY
SIIF
VLGV
IAN
GYVL
W--
---M
VNLT
MAD
LLFL
VTLP
LWIV
YN
LAGC
LFFI
NTY
CSVA
FLGV
I---
--ITL
SLVI
WVT
IVAA
ASYF
LFM
---H
IFIV
CSFF
LVFF
IILVC
NLV
-----
RALW
MVC
TVLA
VFIIC
FVPH
H----
----
---Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Frog
---VT
YSIIF
VFGF
IAN
CYVL
WIF
-IFM
INLT
IADL
LFLV
TLPL
WI--
NVA
GCFF
FLN
TYCS
VAFL
GVI--
----
CISV
AVW
ILLF
ASSL
YFLI
FP--H
FILV
GAFF
VVFL
IILTC
NLV
-----
RALW
MVV
TVLA
VFVL
CFVP
HH---
-----
------
LCLM
STN
CMLD
PIIY
CFLT
KKGo
at-F
PIFY
SIVF
VLGV
IAN
SYVL
W--
---M
VNLT
MAD
LLFL
VTLP
LWIV
YN
LAGC
FFFI
NTY
CSVA
FLAV
I---
--FLL
SLIIW
VSIV
GAAS
YFFV
L--IH
IFLV
FSFF
LVFL
IILFC
NL-
-----
RALW
MVC
TVLA
VFVI
CFVP
HH---
-----
----V
TLCL
LSTN
CVLD
PIIY
CFLT
--Gu
inea
pig
-FPI
VYSI
IFVL
GIIA
NGY
VLW
----F
MVN
LTVA
DLLF
LITL
PLW
IV-
NLA
GCLF
FIN
TYCS
VAFL
GVI--
---I
ALSL
VIW
VAIV
AAAS
YFLV
M-
-IHIC
IVLG
FFIV
FLLI
LFCN
L----
--RA
LWM
VCTV
LAVF
VICF
VPHH
-----
------
QVT
LCLL
STN
CVLD
PVIY
CFL-
--He
dgeh
og-F
PIFY
SIIF
ALGV
IAN
SYVL
W--
--FM
VNLT
LADL
LFLV
TLPL
WII-
NLA
GCFF
FIN
TYCS
VAFL
GVI--
--G
IILSL
VIW
VAIV
SAAS
YFFF
---IH
IFLV
VSFF
VVFL
IIFFC
NL-
-----
RALW
MVC
TVLA
VFIIC
FVPH
H----
----
---Q
ITLC
LLST
NCV
LDPI
IYCF
L---
Hors
e--P
IVYS
IIFVL
GVIA
NSY
VLW
V-N
LTGC
CFFI
NTY
CSVA
FLGV
I---
-IFM
VNLT
VADL
LFLL
TLPL
WV-
---I
SLSL
VIW
LAIV
AAAS
YFLF
M-
-IHIF
LVFS
FFLV
FLIIL
VCN
L----
--RA
LWM
VCTV
LAVF
IICFV
PHH-
----
------
QVT
LCLL
STN
CVLD
PIIY
CFL-
--M
edak
a-F
SVVY
SIFF
VVGL
LAN
VYVL
F--
--YM
TNLT
IADL
LFVC
ALPF
WIG
-RG
IIVSV
LIW
LCLV
SSAI
PSL-
----T
GSFF
FIN
TYGT
ILFL
GAIS
L---H
FLIIG
LFFV
VFFL
VMVC
NLL
-----
---Q
MLL
AVVG
VFLL
CFLP
HHVV
Q--
------
QIT
LVLM
GLN
CILD
PVIY
YFA-
--M
onke
y-F
PIVY
SIIF
VLGV
IAN
GYVL
W--
--FM
VNLT
MAD
MLF
LITL
PLW
IV-
NLA
GCLF
FIN
TYCS
VAFL
GVI--
---I
SLSL
VIW
VAIV
GAAS
YFFI
L--IH
IFIV
FSFF
LVFL
IILFC
NL-
-----
RALW
MVC
TVLA
VFIIC
FVPH
H----
----
---Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Mou
se-F
PIVY
SVIF
ILGV
VAN
GYVL
W--
--FM
VNLT
MAD
LLFL
ITLP
LWIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
-GIS
LSLI
IWVS
IVAT
ASYF
LA--
VVHV
FIAF
CFFL
VFFL
IFYC
N---
----
RALW
MVC
TVLA
VFIIC
FVPH
H----
----
----IT
LCLL
STN
CVLD
PVIY
CFLT
--O
poss
um-F
PIVY
SIIF
VLGF
VSN
AYVL
W--
---M
VNLT
VADL
LFLF
ALPL
WIV
YN
VAGC
LFFI
NTY
CSVC
FLGV
I---
--HVF
IVLC
FFIV
FLVI
LVCN
VI---
---G
ILLS
TIIW
VVIV
GCAS
YFLI
----L
WM
VCTV
MAV
FFIC
FVPH
HVV-
-----
---Q
VTLC
LMSI
NCV
LDPI
IYCF
L---
Ora
ngut
an-F
PIVY
SIIF
VLGV
IAN
GYVL
W--
--FM
VNLT
MAD
MLF
LITL
PLW
IV-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
---I
SLSL
VIW
VAIV
GAAS
YFLI
L--IH
IFIV
FSFF
LVFL
IILFC
NL-
-----
RALW
MVC
TVLA
VFIIC
FVPH
H----
----
----V
TLCL
LSTN
CVLD
PVIY
CFLT
--Pi
g-F
PIFY
SIIF
VLGV
IAN
SYVL
W--
---M
LNLT
MAD
LLFL
VTLP
LWIIY
NLA
GCFF
FIN
TYCS
VSFL
AVI--
---I
SLSL
IIWVA
MVA
AASY
FFVL
--IH
IFLV
FSFF
LVFL
VILF
CNL-
-----
RALW
MVC
TVLA
VFIIC
FVPH
H----
----
----IT
LCLL
STN
CVLD
PIIY
CFLT
--Pi
ka-F
PIVY
SIIF
VLGV
VAN
GYVL
W--
--FM
VNLT
VADL
LFLI
TLPL
WII-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
---I
SLSL
IIWVV
ILAI
ASYF
LIM
--IH
IFIV
FCFF
VVFL
IILVC
NL-
-----
------
QVT
LCLL
STN
CVLD
PVIY
CFL-
--RA
LWM
VCTV
LGVF
IICFV
PHH-
----
Plat
ypus
---VA
YSIIF
VLGI
FAN
GYVL
WVF
--FM
VNLT
VADL
LFLV
TMPL
WIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
-GIV
LSLV
IWVL
IVSS
ALYF
LV--
-IHIF
IVAS
FFAV
FLLI
LVCN
L----
--RA
LWM
VCTV
LAVF
IICFV
PHH-
----
------
-VTL
CLLS
TNCV
LDPV
IYCF
LT--
Puffe
rfish
---M
TNLS
IADL
LFVC
ALPF
WIG
Y--P
SAYG
VIFI
LGLL
ANLY
VLFI
----
GSLF
FIN
TYCS
VLFL
GAIS
VN-G
IIISV
IIWVL
TISM
AIPY
LV--
---FA
IIGLF
FVVF
ALVL
VCN
LLI--
-----
--LLA
VVGV
FVVC
FLPH
HVIQ
GA---
---Q
ITLL
LMGL
NCI
LDPV
VYCF
A---
Rabb
it--P
IVYS
IIFVL
GVVA
NGY
VLW
V---F
MVN
LTVA
DLLF
LITL
PLW
II-N
LAGC
LFFI
NTY
CSVA
FLGV
I---
--IAL
SLVI
WVS
ISAA
ATYF
LIL-
-IHIF
IVAS
FFIV
FLIIL
ICN
L----
--RA
LWM
VCTV
LAVF
IICFV
PHH-
----
------
QVT
LCLL
STN
CVLD
PVIY
CFL-
--Ra
t--P
IVYS
VIFV
LGVV
ANGY
VLW
V---F
MVN
LTVA
DLLF
LMTL
PLW
IV-
NLA
GCLF
FIN
TYCS
VAFL
GVI--
--G
ITLS
LVIW
ISIA
ATAS
YFLA
---V
HIFI
TSCF
FLVF
FLIF
YCN
M---
---RA
LWM
VCTV
LAVF
VICF
VPHH
-----
------
-ITLC
LLST
NCV
LDPV
IYCF
LT--
Shre
w-F
PIFY
SIIF
VLGV
IAN
SYVL
W--
--FM
VNLT
MAD
LLFL
ITLP
LWIV
-N
LTGC
FFFI
NTY
CSVA
FLGV
I---
-GIY
LSLV
IWM
AIVS
AASY
FFF-
--IH
IFLV
CSFF
LVFL
LIFF
CNL-
-----
RALW
MVC
TVLA
VFSI
CFVP
HH---
-----
----V
TLCL
LSTN
CVLD
PIIY
CFLT
--Sq
uirr
elLF
PIIY
SIIF
VVGV
IAN
GYVL
-----F
MLN
LTVA
DLLF
LITL
PLW
II-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
---YL
SLVI
WVF
IVAA
ASYF
LILD
VIHI
FIVF
SFFL
VFLI
ILFC
K----
---RA
LWM
VCAV
LAVF
VICF
VPHH
-----
------
QVT
LCLL
STN
CVLD
PVIY
CFL-
--Sti
ckleba
ck-F
PVVY
SIIF
ILGI
TAN
LYVL
F--
CIYM
INLT
IGDL
LFVT
FLPF
W---
--SGS
LFFI
NTY
SSVL
FLCA
ISV-
-GIIL
SVFI
WLF
TLAT
AILF
LI--
--HLT
IVIG
FFLV
FFVV
VVCN
FL---
-----
QM
LFSV
VGVF
VVCF
LPHH
ACQ
-----
---Q
ITW
LLM
SLN
CILD
PVVY
FFA-
--Zebrafi
sh--P
IFYG
VVFV
LGLI
ANCY
ALYV
--IY
MTN
LTVA
DLLF
VSAL
PFW
I--RG
AIIS
VLIW
AFIL
SASI
PQL-
-----
GTFF
FIN
TYGS
ILFL
TVIS
INIT
HFSI
IGLF
FLVF
LMVI
ICN
------
---L
RMLC
AVIG
VFVI
CFLP
HHVV
------
---Q
ITLM
LMGI
NCL
LDPL
VYCF
A---
Sequ
ence
Dom
ain
ID
omai
n II
Dom
ain
III
Dom
ain
IVD
omai
n V
Dom
ain
VI
Dom
ain
VII
Tc00
.104
7053
5098
49.3
0--Q
IVLL
LLLL
LLLL
LLLL
LLLL
-IF
FTLV
LLFC
SWGM
LLQ
MAM
V---
REFS
SVAL
GIAA
HAVL
LLSE
N---
----F
FLIP
FTVI
LAL-
-HFL
VHLM
IS---
----L
SRFL
ADFI
MFF
CGCA
VLLG
L--I
AMTC
GCLM
IMFF
LLIA
PSFF
---KN
GVIP
PASV
LVLV
LDAV
SRQ
------
---Hu
man
-FPI
VYSI
IFVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLVI
WVA
IVGA
ASYF
LIL-
----IH
IFIV
FSFF
LVFL
IILFC
NL-
-----
RALW
MVC
TVLA
VFIIC
FVPH
H----
----
----V
TLCL
LSTN
CVLD
PVIY
CFLT
--Ch
icke
n--T
VFYS
IIFIL
GFVA
NCY
VLW
I---F
MLN
LTVA
DLLF
LVTM
PLW
IV-
SVAG
SLFF
VNTY
ASVA
FLM
VI---
-GIF
LSAA
IWIIT
VGSA
LYYI
F----
-AI
HIIIC
TLFY
VIFL
FILT
WN
------
-RA
LWM
VCTV
LVVF
IISFV
PHH-
----
------
-VTL
CLLS
MN
CVLD
PVIY
CFLT
--Ch
impa
nzee
-FPI
VYSI
IFVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLVI
WVA
IVGA
ASYF
LIL-
----IH
IFIV
FSFF
LVFL
IILFC
NL-
-----
RALW
MVC
TVLA
VFIIC
FVPH
H----
----
----V
TLCL
LSTN
CVLD
PVIY
CFLT
--Co
w-F
PIFY
SIVF
VLGV
IAN
SYVL
W--
---M
VNLT
MAD
LLFL
VTLP
LWIV
YN
LAGC
FFFI
NTY
CSVA
FLAV
I---
--ILL
SLIIW
VSIV
GAAS
YFFV
L----
-IHIF
LVFS
FFLV
FLIIL
FCN
L----
--RA
LWM
VCTV
LAVF
IICFV
PHH-
----
------
-VTL
CLLS
TNCV
LDPI
IYCF
LT--
Elep
hant
-FPI
FYSI
IFVL
GVIA
NGY
VLW
-----
MVN
LTM
ADLL
FLVT
LPLW
IVY
NLA
GCLF
FIN
TYCS
VAFL
GVI--
---I
TLSL
VIW
VTIV
AAAS
YFLF
M---
---H
IFIV
CSFF
LVFF
IILVC
NLV
-----
RALW
MVC
TVLA
VFIIC
FVPH
H----
----
---Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Frog
---VT
YSIIF
VFGF
IAN
CYVL
WIF
-IFM
INLT
IADL
LFLV
TLPL
WI--
NVA
GCFF
FLN
TYCS
VAFL
GVI--
----
CISV
AVW
ILLF
ASSL
YFLI
FP---
--HFI
LVGA
FFVV
FLIIL
TCN
LV---
--RA
LWM
VVTV
LAVF
VLCF
VPHH
-----
------
---LC
LMST
NCM
LDPI
IYCF
LTKK
Goat
-FPI
FYSI
VFVL
GVIA
NSY
VLW
-----
MVN
LTM
ADLL
FLVT
LPLW
IVY
NLA
GCFF
FIN
TYCS
VAFL
AVI--
---F
LLSL
IIWVS
IVGA
ASYF
FVL-
----IH
IFLV
FSFF
LVFL
IILFC
NL-
-----
RALW
MVC
TVLA
VFVI
CFVP
HH---
-----
----V
TLCL
LSTN
CVLD
PIIY
CFLT
--Gu
inea
pig
-FPI
VYSI
IFVL
GIIA
NGY
VLW
----F
MVN
LTVA
DLLF
LITL
PLW
IV-
NLA
GCLF
FIN
TYCS
VAFL
GVI--
---I
ALSL
VIW
VAIV
AAAS
YFLV
M---
--IH
ICIV
LGFF
IVFL
LILF
CNL-
-----
RALW
MVC
TVLA
VFVI
CFVP
HH---
-----
---Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Hedg
ehog
-FPI
FYSI
IFAL
GVIA
NSY
VLW
----F
MVN
LTLA
DLLF
LVTL
PLW
II-N
LAGC
FFFI
NTY
CSVA
FLGV
I---
-GIIL
SLVI
WVA
IVSA
ASYF
FF---
---IH
IFLV
VSFF
VVFL
IIFFC
NL-
-----
RALW
MVC
TVLA
VFIIC
FVPH
H----
----
---Q
ITLC
LLST
NCV
LDPI
IYCF
L---
Hors
e--P
IVYS
IIFVL
GVIA
NSY
VLW
V--IF
MVN
LTVA
DLLF
LLTL
PLW
V--
NLT
GCCF
FIN
TYCS
VAFL
GVI--
---I
SLSL
VIW
LAIV
AAAS
YFLF
M---
--IH
IFLV
FSFF
LVFL
IILVC
NL-
-----
RALW
MVC
TVLA
VFIIC
FVPH
H----
----
---Q
VTLC
LLST
NCV
LDPI
IYCF
L---
Med
aka
-FSV
VYSI
FFVV
GLLA
NVY
VLF-
---Y
MTN
LTIA
DLLF
VCAL
PFW
IG-
--TGS
FFFI
NTY
GTIL
FLGA
ISL-
RGIIV
SVLI
WLC
LVSS
AIPS
L----
----H
FLIIG
LFFV
VFFL
VMVC
NLL
-----
---Q
MLL
AVVG
VFLL
CFLP
HHVV
Q--
------
QIT
LVLM
GLN
CILD
PVIY
YFA-
--M
onke
y-F
PIVY
SIIF
VLGV
IAN
GYVL
W--
--FM
VNLT
MAD
MLF
LITL
PLW
IV-
NLA
GCLF
FIN
TYCS
VAFL
GVI--
---I
SLSL
VIW
VAIV
GAAS
YFFI
L----
-IHIF
IVFS
FFLV
FLIIL
FCN
L----
--RA
LWM
VCTV
LAVF
IICFV
PHH-
----
------
QVT
LCLL
STN
CVLD
PVIY
CFL-
--M
ouse
-FPI
VYSV
IFIL
GVVA
NGY
VLW
----F
MVN
LTM
ADLL
FLIT
LPLW
IV-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
--G
ISLS
LIIW
VSIV
ATAS
YFLA
-----
VVHV
FIAF
CFFL
VFFL
IFYC
N---
----
RALW
MVC
TVLA
VFIIC
FVPH
H----
----
----IT
LCLL
STN
CVLD
PVIY
CFLT
--O
poss
um-F
PIVY
SIIF
VLGF
VSN
AYVL
W--
---M
VNLT
VADL
LFLF
ALPL
WIV
YN
VAGC
LFFI
NTY
CSVC
FLGV
I---
-GIL
LSTI
IWVV
IVGC
ASYF
LI---
----H
VFIV
LCFF
IVFL
VILV
CNVI
-----
--LW
MVC
TVM
AVFF
ICFV
PHHV
V---
------
QVT
LCLM
SIN
CVLD
PIIY
CFL-
--O
rang
utan
-FPI
VYSI
IFVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLVI
WVA
IVGA
ASYF
LIL-
----IH
IFIV
FSFF
LVFL
IILFC
NL-
-----
RALW
MVC
TVLA
VFIIC
FVPH
H----
----
----V
TLCL
LSTN
CVLD
PVIY
CFLT
--Pi
g-F
PIFY
SIIF
VLGV
IAN
SYVL
W--
---M
LNLT
MAD
LLFL
VTLP
LWIIY
NLA
GCFF
FIN
TYCS
VSFL
AVI--
---I
SLSL
IIWVA
MVA
AASY
FFVL
----
-IHIF
LVFS
FFLV
FLVI
LFCN
L----
--RA
LWM
VCTV
LAVF
IICFV
PHH-
----
------
-ITLC
LLST
NCV
LDPI
IYCF
LT--
Pika
-FPI
VYSI
IFVL
GVVA
NGY
VLW
----F
MVN
LTVA
DLLF
LITL
PLW
II-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLIIW
VVIL
AIAS
YFLI
M---
--IH
IFIV
FCFF
VVFL
IILVC
NL-
-----
RALW
MVC
TVLG
VFIIC
FVPH
H----
----
---Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Plat
ypus
---VA
YSIIF
VLGI
FAN
GYVL
WVF
--FM
VNLT
VADL
LFLV
TMPL
WIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
-IHIF
IVAS
FFAV
FLLI
LVCN
L----
---G
IVLS
LVIW
VLIV
SSAL
YFLV
-----
RALW
MVC
TVLA
VFIIC
FVPH
H----
----
----V
TLCL
LSTN
CVLD
PVIY
CFLT
--Pu
fferfish
--PSA
YGVI
FILG
LLAN
LYVL
FI-
---M
TNLS
IADL
LFVC
ALPF
WIG
Y---
GSLF
FIN
TYCS
VLFL
GAIS
VN-G
IIISV
IIWVL
TISM
AIPY
LV---
-----
FAIIG
LFFV
VFAL
VLVC
NLL
I----
-----L
LAVV
GVFV
VCFL
PHHV
IQGA
------
QIT
LLLM
GLN
CILD
PVVY
CFA-
--Ra
bbit
--PIV
YSIIF
VLGV
VAN
GYVL
WV-
--FM
VNLT
VADL
LFLI
TLPL
WII-
NLA
GCLF
FIN
TYCS
VAFL
GVI--
---I
ALSL
VIW
VSIS
AAAT
YFLI
L----
-IHIF
IVAS
FFIV
FLIIL
ICN
L----
--RA
LWM
VCTV
LAVF
IICFV
PHH-
----
------
QVT
LCLL
STN
CVLD
PVIY
CFL-
--Ra
t--P
IVYS
VIFV
LGVV
ANGY
VLW
V---F
MVN
LTVA
DLLF
LMTL
PLW
IV-
NLA
GCLF
FIN
TYCS
VAFL
GVI--
--G
ITLS
LVIW
ISIA
ATAS
YFLA
-----
-VHI
FITS
CFFL
VFFL
IFYC
NM
------
RALW
MVC
TVLA
VFVI
CFVP
HH---
-----
----IT
LCLL
STN
CVLD
PVIY
CFLT
--Sh
rew
-FPI
FYSI
IFVL
GVIA
NSY
VLW
----F
MVN
LTM
ADLL
FLIT
LPLW
IV-
NLT
GCFF
FIN
TYCS
VAFL
GVI--
--G
IYLS
LVIW
MAI
VSAA
SYFF
F----
--IH
IFLV
CSFF
LVFL
LIFF
CNL-
-----
RALW
MVC
TVLA
VFSI
CFVP
HH---
-----
----V
TLCL
LSTN
CVLD
PIIY
CFLT
--Sq
uirr
el--F
MLN
LTVA
DLLF
LITL
PLW
II-LF
PIIY
SIIF
VVGV
IAN
GYVL
---N
VAGC
LFFI
NTY
CSVA
FLGV
I---
---YL
SLVI
WVF
IVAA
ASYF
LILD
---VI
HIFI
VFSF
FLVF
LIIL
FCK-
------
RALW
MVC
AVLA
VFVI
CFVP
HH---
-----
---Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Stickleba
ck-F
PVVY
SIIF
ILGI
TAN
LYVL
F--
--SGS
LFFI
NTY
SSVL
FLCA
ISV-
CIYM
INLT
IGDL
LFVT
FLPF
W---
-GIIL
SVFI
WLF
TLAT
AILF
LI---
-----
QM
LFSV
VGVF
VVCF
LPHH
ACQ
----H
LTIV
IGFF
LVFF
VVVV
CNFL
-----
------
QIT
WLL
MSL
NCI
LDPV
VYFF
A---
Zebrafi
sh-IY
MTN
LTVA
DLLF
VSAL
PFW
I----P
IFYG
VVFV
LGLI
ANCY
ALYV
----
GTFF
FIN
TYGS
ILFL
TVIS
INRG
AIIS
VLIW
AFIL
SASI
PQL-
-----
--LRM
LCAV
IGVF
VICF
LPHH
VV---
ITHF
SIIG
LFFL
VFLM
VIIC
N---
----
------
QIT
LMLM
GIN
CLLD
PLVY
CFA-
--
13Supplementary data Search for PAF receptor in T. cruzi • Daniel Fábio Kawano et al.
Sequ
ence
Dom
ain
ID
omai
n II
Dom
ain
III
Dom
ain
IVD
omai
n V
Dom
ain
VI
Dom
ain
VII
Tc00
.104
7053
5107
29.2
90---
-TDV
CIFI
LNFL
SLLV
AFKL
YL-
--ELQ
PFSC
CPLI
GKIIF
GVW
DR-
---W
IRYF
TTYF
IVM
LFRA
IVVV
M-N
PVKN
VIM
TLVT
FGSG
--FIH
CG-F
SGHT
VPIT
LSLL
VQW
IYGS
M--
-----V
FRPA
SVLL
VLLS
FYSI
IASR
YTDD
ILVSF
YITV
TTFL
VLRH
---Hu
man
-FPI
VYSI
IFVL
GVIA
--NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLVI
WVA
IVGA
ASYF
LIL-
-IHIF
IVFS
FFLV
FLIIL
FCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
--V
TLCL
LSTN
CVLD
PVIY
CFLT
--Ch
icke
n--T
VFYS
IIFIL
GFVA
--NCY
VLW
I---F
MLN
LTVA
DLLF
LVTM
PLW
IV-
SVAG
SLFF
VNTY
ASVA
FLM
VI---
-GIF
LSAA
IWIIT
VGSA
LYYI
F--
AIHI
IICTL
FYVI
FLFI
LTW
N---
RALW
MVC
TVLV
VFIIS
FVPH
H----
--V
TLCL
LSM
NCV
LDPV
IYCF
LT--
Chim
panz
ee-F
PIVY
SIIF
VLGV
IA--N
GYVL
W--
--FM
VNLT
MAD
MLF
LITL
PLW
IV-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
---I
SLSL
VIW
VAIV
GAAS
YFLI
L--IH
IFIV
FSFF
LVFL
IILFC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
-VTL
CLLS
TNCV
LDPV
IYCF
LT--
Cow
-FPI
FYSI
VFVL
GVIA
--NSY
VLW
-----
MVN
LTM
ADLL
FLVT
LPLW
IVY
NLA
GCFF
FIN
TYCS
VAFL
AVI--
---I
LLSL
IIWVS
IVGA
ASYF
FVL-
-IHIF
LVFS
FFLV
FLIIL
FCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
--V
TLCL
LSTN
CVLD
PIIY
CFLT
--El
epha
nt-F
PIFY
SIIF
VLGV
IA--N
GYVL
W--
---M
VNLT
MAD
LLFL
VTLP
LWIV
YN
LAGC
LFFI
NTY
CSVA
FLGV
I---
--ITL
SLVI
WVT
IVAA
ASYF
LFM
---H
IFIV
CSFF
LVFF
IILVC
NLV
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
QVT
LCLL
STN
CVLD
PVIY
CFL-
--Fr
og---
VTYS
IIFVF
GFIA
--NCY
VLW
IF-IF
MIN
LTIA
DLLF
LVTL
PLW
I--N
VAGC
FFFL
NTY
CSVA
FLGV
I---
---CI
SVAV
WIL
LFAS
SLYF
LIFP
--HFI
LVGA
FFVV
FLIIL
TCN
LV-
RALW
MVV
TVLA
VFVL
CFVP
HH---
-----
LCLM
STN
CMLD
PIIY
CFLT
KKGo
at-F
PIFY
SIVF
VLGV
IA--N
SYVL
W--
---M
VNLT
MAD
LLFL
VTLP
LWIV
YN
LAGC
FFFI
NTY
CSVA
FLAV
I---
--FLL
SLIIW
VSIV
GAAS
YFFV
L--IH
IFLV
FSFF
LVFL
IILFC
NL-
-RA
LWM
VCTV
LAVF
VICF
VPHH
-----
-VTL
CLLS
TNCV
LDPI
IYCF
LT--
Guin
ea p
ig-F
PIVY
SIIF
VLGI
IA--N
GYVL
W--
--FM
VNLT
VADL
LFLI
TLPL
WIV
-N
LAGC
LFFI
NTY
CSVA
FLGV
I---
--IAL
SLVI
WVA
IVAA
ASYF
LVM
--IH
ICIV
LGFF
IVFL
LILF
CNL-
-RA
LWM
VCTV
LAVF
VICF
VPHH
-----
QVT
LCLL
STN
CVLD
PVIY
CFL-
--He
dgeh
og-F
PIFY
SIIF
ALGV
IA--N
SYVL
W--
--FM
VNLT
LADL
LFLV
TLPL
WII-
NLA
GCFF
FIN
TYCS
VAFL
GVI--
--G
IILSL
VIW
VAIV
SAAS
YFFF
---IH
IFLV
VSFF
VVFL
IIFFC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
QIT
LCLL
STN
CVLD
PIIY
CFL-
--Ho
rse
--PIV
YSIIF
VLGV
IA--N
SYVL
WV-
-IFM
VNLT
VADL
LFLL
TLPL
WV-
-N
LTGC
CFFI
NTY
CSVA
FLGV
I---
--ISL
SLVI
WLA
IVAA
ASYF
LFM
--IH
IFLV
FSFF
LVFL
IILVC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
QVT
LCLL
STN
CVLD
PIIY
CFL-
--M
edak
a-F
SVVY
SIFF
VVGL
LA--N
VYVL
F--
--YM
TNLT
IADL
LFVC
ALPF
WIG
---T
GSFF
FIN
TYGT
ILFL
GAIS
L-RG
IIVSV
LIW
LCLV
SSAI
PSL-
----H
FLIIG
LFFV
VFFL
VMVC
NLL
----
QM
LLAV
VGVF
LLCF
LPHH
VVQ
--Q
ITLV
LMGL
NCI
LDPV
IYYF
A---
Mon
key
-FPI
VYSI
IFVL
GVIA
--NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
-N
LAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLVI
WVA
IVGA
ASYF
FIL-
-IHIF
IVFS
FFLV
FLIIL
FCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
-Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Mou
se-F
PIVY
SVIF
ILGV
VA--N
GYVL
W--
--FM
VNLT
MAD
LLFL
ITLP
LWIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
-GIS
LSLI
IWVS
IVAT
ASYF
LA--
VVHV
FIAF
CFFL
VFFL
IFYC
N---
RALW
MVC
TVLA
VFIIC
FVPH
H----
--IT
LCLL
STN
CVLD
PVIY
CFLT
--O
poss
um-F
PIVY
SIIF
VLGF
VS--N
AYVL
W--
---M
VNLT
VADL
LFLF
ALPL
WIV
YN
VAGC
LFFI
NTY
CSVC
FLGV
I---
-GIL
LSTI
IWVV
IVGC
ASYF
LI--
--HVF
IVLC
FFIV
FLVI
LVCN
VI-
--LW
MVC
TVM
AVFF
ICFV
PHHV
V---
QVT
LCLM
SIN
CVLD
PIIY
CFL-
--O
rang
utan
-FPI
VYSI
IFVL
GVIA
--NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLVI
WVA
IVGA
ASYF
LIL-
-IHIF
IVFS
FFLV
FLIIL
FCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
--V
TLCL
LSTN
CVLD
PVIY
CFLT
--Pi
g-F
PIFY
SIIF
VLGV
IA--N
SYVL
W--
---M
LNLT
MAD
LLFL
VTLP
LWIIY
NLA
GCFF
FIN
TYCS
VSFL
AVI--
---I
SLSL
IIWVA
MVA
AASY
FFVL
--IH
IFLV
FSFF
LVFL
VILF
CNL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
-ITLC
LLST
NCV
LDPI
IYCF
LT--
Pika
-FPI
VYSI
IFVL
GVVA
--NGY
VLW
----F
MVN
LTVA
DLLF
LITL
PLW
II-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLIIW
VVIL
AIAS
YFLI
M-
-IHIF
IVFC
FFVV
FLIIL
VCN
L--
RALW
MVC
TVLG
VFIIC
FVPH
H----
-Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Plat
ypus
---VA
YSIIF
VLGI
FA--N
GYVL
WVF
--FM
VNLT
VADL
LFLV
TMPL
WIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
-GIV
LSLV
IWVL
IVSS
ALYF
LV--
-IHIF
IVAS
FFAV
FLLI
LVCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
--V
TLCL
LSTN
CVLD
PVIY
CFLT
--Pu
fferfish
--PSA
YGVI
FILG
LLA-
-NLY
VLFI
----
MTN
LSIA
DLLF
VCAL
PFW
IGY
---GS
LFFI
NTY
CSVL
FLGA
ISVN
-GIII
SVIIW
VLTI
SMAI
PYLV
-----
FAIIG
LFFV
VFAL
VLVC
NLL
I---
--LLA
VVGV
FVVC
FLPH
HVIQ
GAQ
ITLL
LMGL
NCI
LDPV
VYCF
A---
Rabb
it--P
IVYS
IIFVL
GVVA
--NGY
VLW
V---F
MVN
LTVA
DLLF
LITL
PLW
II-N
LAGC
LFFI
NTY
CSVA
FLGV
I---
--IAL
SLVI
WVS
ISAA
ATYF
LIL-
-IHIF
IVAS
FFIV
FLIIL
ICN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
-Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Rat
--PIV
YSVI
FVLG
VVA-
-NGY
VLW
V---F
MVN
LTVA
DLLF
LMTL
PLW
IV-
NLA
GCLF
FIN
TYCS
VAFL
GVI--
--G
ITLS
LVIW
ISIA
ATAS
YFLA
---V
HIFI
TSCF
FLVF
FLIF
YCN
M--
RALW
MVC
TVLA
VFVI
CFVP
HH---
---IT
LCLL
STN
CVLD
PVIY
CFLT
--Sh
rew
-FPI
FYSI
IFVL
GVIA
--NSY
VLW
----F
MVN
LTM
ADLL
FLIT
LPLW
IV-
NLT
GCFF
FIN
TYCS
VAFL
GVI--
--G
IYLS
LVIW
MAI
VSAA
SYFF
F--
-IHIF
LVCS
FFLV
FLLI
FFCN
L--
RALW
MVC
TVLA
VFSI
CFVP
HH---
---V
TLCL
LSTN
CVLD
PIIY
CFLT
--Sq
uirr
elLF
PIIY
SIIF
VVGV
IA--N
GYVL
-----F
MLN
LTVA
DLLF
LITL
PLW
II-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
---YL
SLVI
WVF
IVAA
ASYF
LILD
VIHI
FIVF
SFFL
VFLI
ILFC
K---
RALW
MVC
AVLA
VFVI
CFVP
HH---
--Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Stickleba
ck-F
PVVY
SIIF
ILGI
TA--N
LYVL
F--
CIYM
INLT
IGDL
LFVT
FLPF
W---
--SGS
LFFI
NTY
SSVL
FLCA
ISV-
-GIIL
SVFI
WLF
TLAT
AILF
LI--
--HLT
IVIG
FFLV
FFVV
VVCN
FL-
---Q
MLF
SVVG
VFVV
CFLP
HHAC
Q--
QIT
WLL
MSL
NCI
LDPV
VYFF
A---
Zebrafi
sh--P
IFYG
VVFV
LGLI
A--N
CYAL
YV-
-IYM
TNLT
VADL
LFVS
ALPF
WI--
---GT
FFFI
NTY
GSIL
FLTV
ISIN
RGAI
ISVL
IWAF
ILSA
SIPQ
L---
ITHF
SIIG
LFFL
VFLM
VIIC
N---
--LRM
LCAV
IGVF
VICF
LPHH
VV---
QIT
LMLM
GIN
CLLD
PLVY
CFA-
--
Sequ
ence
Dom
ain
ID
omai
n II
Dom
ain
III
Dom
ain
IVD
omai
n V
Dom
ain
VI
Dom
ain
VII
Tc00
.104
7053
5041
37.1
40SH
GVGL
ILFG
VLSI
VVFD
ALL-
---F
IYGT
FCFA
CMM
CMGC
SSIY
H--
YYGI
SVLI
VASF
LPPL
YIGF
Y---
QAL
YMGC
ACVF
GGLC
LAM
AAL-
--RI
MTY
VATV
MCG
LVPT
LHYV
F----
-Q
TFVG
VFLM
FLFY
GMGV
LFYC
------
----
QIW
HVFV
LVAA
CV--H
FFTC
ISI--
Hum
an-F
PIVY
SIIF
VLGV
IAN
GYVL
W--
--FM
VNLT
MAD
MLF
LITL
PLW
IV-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
---I
SLSL
VIW
VAIV
GAAS
YFLI
L----
IHIF
IVFS
FFLV
FLIIL
FCN
L--
-----R
ALW
MVC
TVLA
VFIIC
FVPH
H----
--V
-TLC
LLST
NCV
LDPV
IYCF
LT--
Chic
ken
--TVF
YSIIF
ILGF
VAN
CYVL
WI-
--FM
LNLT
VADL
LFLV
TMPL
WIV
-SV
AGSL
FFVN
TYAS
VAFL
MVI
----G
IFLS
AAIW
IITVG
SALY
YIF-
---A
IHIII
CTLF
YVIF
LFILT
WN
------
--RAL
WM
VCTV
LVVF
IISFV
PHH-
----
-V-T
LCLL
SMN
CVLD
PVIY
CFLT
--Ch
impa
nzee
-FPI
VYSI
IFVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
---I
SLSL
VIW
VAIV
GAAS
YFLI
L-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
---IH
IFIV
FSFF
LVFL
IILFC
NL-
--V
-TLC
LLST
NCV
LDPV
IYCF
LT--
-----R
ALW
MVC
TVLA
VFIIC
FVPH
H----
-Co
w-F
PIFY
SIVF
VLGV
IAN
SYVL
W--
---M
VNLT
MAD
LLFL
VTLP
LWIV
YN
LAGC
FFFI
NTY
CSVA
FLAV
I---
--ILL
SLIIW
VSIV
GAAS
YFFV
L----
IHIF
LVFS
FFLV
FLIIL
FCN
L--
-----R
ALW
MVC
TVLA
VFIIC
FVPH
H----
--V
-TLC
LLST
NCV
LDPI
IYCF
LT--
Elep
hant
-FPI
FYSI
IFVL
GVIA
NGY
VLW
-----
MVN
LTM
ADLL
FLVT
LPLW
IVY
NLA
GCLF
FIN
TYCS
VAFL
GVI--
---I
TLSL
VIW
VTIV
AAAS
YFLF
M-
----H
IFIV
CSFF
LVFF
IILVC
NLV
----
--RAL
WM
VCTV
LAVF
IICFV
PHH-
----
QV-
TLCL
LSTN
CVLD
PVIY
CFL-
--Fr
og---
VTYS
IIFVF
GFIA
NCY
VLW
IF-IF
MIN
LTIA
DLLF
LVTL
PLW
I--N
VAGC
FFFL
NTY
CSVA
FLGV
I---
---CI
SVAV
WIL
LFAS
SLYF
LIFP
----H
FILV
GAFF
VVFL
IILTC
NLV
----
--RAL
WM
VVTV
LAVF
VLCF
VPHH
-----
----L
CLM
STN
CMLD
PIIY
CFLT
KKGo
at-F
PIFY
SIVF
VLGV
IAN
SYVL
W--
---M
VNLT
MAD
LLFL
VTLP
LWIV
YN
LAGC
FFFI
NTY
CSVA
FLAV
I---
---IH
IFLV
FSFF
LVFL
IILFC
NL-
---F
LLSL
IIWVS
IVGA
ASYF
FVL-
-----R
ALW
MVC
TVLA
VFVI
CFVP
HH---
---V
-TLC
LLST
NCV
LDPI
IYCF
LT--
Guin
ea p
ig-F
PIVY
SIIF
VLGI
IAN
GYVL
W--
--FM
VNLT
VADL
LFLI
TLPL
WIV
-N
LAGC
LFFI
NTY
CSVA
FLGV
I---
--IAL
SLVI
WVA
IVAA
ASYF
LVM
----
IHIC
IVLG
FFIV
FLLI
LFCN
L--
-----R
ALW
MVC
TVLA
VFVI
CFVP
HH---
--Q
V-TL
CLLS
TNCV
LDPV
IYCF
L---
Hedg
ehog
-FPI
FYSI
IFAL
GVIA
NSY
VLW
--N
LAGC
FFFI
NTY
CSVA
FLGV
I---
--FM
VNLT
LADL
LFLV
TLPL
WII-
-GIIL
SLVI
WVA
IVSA
ASYF
FF--
---IH
IFLV
VSFF
VVFL
IIFFC
NL-
----
--RAL
WM
VCTV
LAVF
IICFV
PHH-
----
QI-T
LCLL
STN
CVLD
PIIY
CFL-
--Ho
rse
--PIV
YSIIF
VLGV
IAN
SYVL
WV-
-IFM
VNLT
VADL
LFLL
TLPL
WV-
-N
LTGC
CFFI
NTY
CSVA
FLGV
I---
--ISL
SLVI
WLA
IVAA
ASYF
LFM
----
IHIF
LVFS
FFLV
FLIIL
VCN
L--
-----R
ALW
MVC
TVLA
VFIIC
FVPH
H----
-Q
V-TL
CLLS
TNCV
LDPI
IYCF
L---
Med
aka
-FSV
VYSI
FFVV
GLLA
NVY
VLF-
---Y
MTN
LTIA
DLLF
VCAL
PFW
IG-
--TGS
FFFI
NTY
GTIL
FLGA
ISL-
RGIIV
SVLI
WLC
LVSS
AIPS
L---
----H
FLIIG
LFFV
VFFL
VMVC
NLL
----
-----Q
MLL
AVVG
VFLL
CFLP
HHVV
Q--
QI-T
LVLM
GLN
CILD
PVIY
YFA-
--M
onke
y-F
PIVY
SIIF
VLGV
IAN
GYVL
W--
--FM
VNLT
MAD
MLF
LITL
PLW
IV-
NLA
GCLF
FIN
TYCS
VAFL
GVI--
---I
SLSL
VIW
VAIV
GAAS
YFFI
L----
IHIF
IVFS
FFLV
FLIIL
FCN
L--
-----R
ALW
MVC
TVLA
VFIIC
FVPH
H----
-Q
V-TL
CLLS
TNCV
LDPV
IYCF
L---
Mou
se-F
PIVY
SVIF
ILGV
VAN
GYVL
W--
--FM
VNLT
MAD
LLFL
ITLP
LWIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
-GIS
LSLI
IWVS
IVAT
ASYF
LA--
--VVH
VFIA
FCFF
LVFF
LIFY
CN---
-----R
ALW
MVC
TVLA
VFIIC
FVPH
H----
--I-
TLCL
LSTN
CVLD
PVIY
CFLT
--O
poss
um-F
PIVY
SIIF
VLGF
VSN
AYVL
W--
---M
VNLT
VADL
LFLF
ALPL
WIV
YN
VAGC
LFFI
NTY
CSVC
FLGV
I---
-GIL
LSTI
IWVV
IVGC
ASYF
LI--
----H
VFIV
LCFF
IVFL
VILV
CNVI
----
----L
WM
VCTV
MAV
FFIC
FVPH
HVV-
--Q
V-TL
CLM
SIN
CVLD
PIIY
CFL-
--O
rang
utan
-FPI
VYSI
IFVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLVI
WVA
IVGA
ASYF
LIL-
---IH
IFIV
FSFF
LVFL
IILFC
NL-
----
--RAL
WM
VCTV
LAVF
IICFV
PHH-
----
-V-T
LCLL
STN
CVLD
PVIY
CFLT
--Pi
g-F
PIFY
SIIF
VLGV
IAN
SYVL
W--
---M
LNLT
MAD
LLFL
VTLP
LWIIY
NLA
GCFF
FIN
TYCS
VSFL
AVI--
---I
SLSL
IIWVA
MVA
AASY
FFVL
----
IHIF
LVFS
FFLV
FLVI
LFCN
L--
-----R
ALW
MVC
TVLA
VFIIC
FVPH
H----
--I-
TLCL
LSTN
CVLD
PIIY
CFLT
--Pi
ka-F
PIVY
SIIF
VLGV
VAN
GYVL
W--
--FM
VNLT
VADL
LFLI
TLPL
WII-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
---I
SLSL
IIWVV
ILAI
ASYF
LIM
----
IHIF
IVFC
FFVV
FLIIL
VCN
L--
-----R
ALW
MVC
TVLG
VFIIC
FVPH
H----
-Q
V-TL
CLLS
TNCV
LDPV
IYCF
L---
Plat
ypus
---VA
YSIIF
VLGI
FAN
GYVL
WVF
--FM
VNLT
VADL
LFLV
TMPL
WIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
-GIV
LSLV
IWVL
IVSS
ALYF
LV--
---IH
IFIV
ASFF
AVFL
LILV
CNL-
----
--RAL
WM
VCTV
LAVF
IICFV
PHH-
----
-V-T
LCLL
STN
CVLD
PVIY
CFLT
--Pu
fferfish
--PSA
YGVI
FILG
LLAN
LYVL
FI-
---M
TNLS
IADL
LFVC
ALPF
WIG
Y---
GSLF
FIN
TYCS
VLFL
GAIS
VN-G
IIISV
IIWVL
TISM
AIPY
LV--
-----F
AIIG
LFFV
VFAL
VLVC
NLL
I---
------
-LLA
VVGV
FVVC
FLPH
HVIQ
GAQ
I-TLL
LMGL
NCI
LDPV
VYCF
A---
Rabb
it--F
MVN
LTVA
DLLF
LITL
PLW
II---P
IVYS
IIFVL
GVVA
NGY
VLW
V-N
LAGC
LFFI
NTY
CSVA
FLGV
I---
--IAL
SLVI
WVS
ISAA
ATYF
LIL-
---IH
IFIV
ASFF
IVFL
IILIC
NL-
----
--RAL
WM
VCTV
LAVF
IICFV
PHH-
----
QV-
TLCL
LSTN
CVLD
PVIY
CFL-
--Ra
t--P
IVYS
VIFV
LGVV
ANGY
VLW
V---F
MVN
LTVA
DLLF
LMTL
PLW
IV-
NLA
GCLF
FIN
TYCS
VAFL
GVI--
--G
ITLS
LVIW
ISIA
ATAS
YFLA
-----
VHIF
ITSC
FFLV
FFLI
FYCN
M--
-----R
ALW
MVC
TVLA
VFVI
CFVP
HH---
---I-
TLCL
LSTN
CVLD
PVIY
CFLT
--Sh
rew
-FPI
FYSI
IFVL
GVIA
NSY
VLW
----F
MVN
LTM
ADLL
FLIT
LPLW
IV-
NLT
GCFF
FIN
TYCS
VAFL
GVI--
--G
IYLS
LVIW
MAI
VSAA
SYFF
F--
---IH
IFLV
CSFF
LVFL
LIFF
CNL-
----
--RAL
WM
VCTV
LAVF
SICF
VPHH
-----
-V-T
LCLL
STN
CVLD
PIIY
CFLT
--Sq
uirr
elLF
PIIY
SIIF
VVGV
IAN
GYVL
-----F
MLN
LTVA
DLLF
LITL
PLW
II-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
---YL
SLVI
WVF
IVAA
ASYF
LILD
--VIH
IFIV
FSFF
LVFL
IILFC
K---
-----R
ALW
MVC
AVLA
VFVI
CFVP
HH---
--Q
V-TL
CLLS
TNCV
LDPV
IYCF
L---
Stickleba
ck-F
PVVY
SIIF
ILGI
TAN
LYVL
F--
CIYM
INLT
IGDL
LFVT
FLPF
W---
--SGS
LFFI
NTY
SSVL
FLCA
ISV-
-GIIL
SVFI
WLF
TLAT
AILF
LI--
----H
LTIV
IGFF
LVFF
VVVV
CNFL
----
-----Q
MLF
SVVG
VFVV
CFLP
HHAC
Q--
QI-T
WLL
MSL
NCI
LDPV
VYFF
A---
Zebrafi
sh-IY
MTN
LTVA
DLLF
VSAL
PFW
I----P
IFYG
VVFV
LGLI
ANCY
ALYV
----
GTFF
FIN
TYGS
ILFL
TVIS
INRG
AIIS
VLIW
AFIL
SASI
PQL-
-----
----L
RMLC
AVIG
VFVI
CFLP
HHVV
-----I
THFS
IIGLF
FLVF
LMVI
ICN
---Q
I-TLM
LMGI
NCL
LDPL
VYCF
A---
14Supplementary data Search for PAF receptor in T. cruzi • Daniel Fábio Kawano et al.
Sequ
ence
Dom
ain
ID
omai
n II
Dom
ain
III
Dom
ain
IVD
omai
n V
Dom
ain
VI
Dom
ain
VII
Tc00
.104
7053
5097
99.9
0-H
GVGL
ILFG
VLSI
VVFD
ALLF
---F
IYGT
FCFA
CMM
CMGC
SSIY
H--
YYGI
SVLI
VASF
LPPL
YIGF
Y---
-ALY
MGC
ACVF
GGLC
LAM
AALP
--RI
MTY
VATV
MCG
LVPT
VHYV
F---
QTF
VGVF
LMFL
FYGM
GVLF
YC---
------
-IW
HVFV
LVAA
CV--H
FFTC
ISIY
-Hu
man
-FPI
VYSI
IFVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLVI
WVA
IVGA
ASYF
LIL-
-IHIF
IVFS
FFLV
FLIIL
FCN
L--
-----R
ALW
MVC
TVLA
VFIIC
FVPH
H----
--V
TLCL
LSTN
CVLD
PVIY
CFLT
--Ch
icke
n--T
VFYS
IIFIL
GFVA
NCY
VLW
I---F
MLN
LTVA
DLLF
LVTM
PLW
IV-
SVAG
SLFF
VNTY
ASVA
FLM
VI---
-----R
ALW
MVC
TVLV
VFIIS
FVPH
H----
--G
IFLS
AAIW
IITVG
SALY
YIF-
-AI
HIIIC
TLFY
VIFL
FILT
WN
----V
TLCL
LSM
NCV
LDPV
IYCF
LT--
Chim
panz
ee-F
PIVY
SIIF
VLGV
IAN
GYVL
W--
--FM
VNLT
MAD
MLF
LITL
PLW
IV-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
---I
SLSL
VIW
VAIV
GAAS
YFLI
L--IH
IFIV
FSFF
LVFL
IILFC
NL-
----
--RAL
WM
VCTV
LAVF
IICFV
PHH-
----
-VTL
CLLS
TNCV
LDPV
IYCF
LT--
Cow
-FPI
FYSI
VFVL
GVIA
NSY
VLW
-----
MVN
LTM
ADLL
FLVT
LPLW
IVY
NLA
GCFF
FIN
TYCS
VAFL
AVI--
---I
LLSL
IIWVS
IVGA
ASYF
FVL-
-IHIF
LVFS
FFLV
FLIIL
FCN
L--
-----R
ALW
MVC
TVLA
VFIIC
FVPH
H----
--V
TLCL
LSTN
CVLD
PIIY
CFLT
--El
epha
nt-F
PIFY
SIIF
VLGV
IAN
GYVL
W--
---M
VNLT
MAD
LLFL
VTLP
LWIV
YN
LAGC
LFFI
NTY
CSVA
FLGV
I---
--ITL
SLVI
WVT
IVAA
ASYF
LFM
---H
IFIV
CSFF
LVFF
IILVC
NLV
----
--RAL
WM
VCTV
LAVF
IICFV
PHH-
----
QVT
LCLL
STN
CVLD
PVIY
CFL-
--Fr
og---
VTYS
IIFVF
GFIA
NCY
VLW
IF-IF
MIN
LTIA
DLLF
LVTL
PLW
I--N
VAGC
FFFL
NTY
CSVA
FLGV
I---
--HFI
LVGA
FFVV
FLIIL
TCN
LV-
---CI
SVAV
WIL
LFAS
SLYF
LIFP
-----R
ALW
MVV
TVLA
VFVL
CFVP
HH---
-----
LCLM
STN
CMLD
PIIY
CFLT
KKGo
at-F
PIFY
SIVF
VLGV
IAN
SYVL
W--
---M
VNLT
MAD
LLFL
VTLP
LWIV
YN
LAGC
FFFI
NTY
CSVA
FLAV
I---
--FLL
SLIIW
VSIV
GAAS
YFFV
L--IH
IFLV
FSFF
LVFL
IILFC
NL-
----
--RAL
WM
VCTV
LAVF
VICF
VPHH
-----
-VTL
CLLS
TNCV
LDPI
IYCF
LT--
Guin
ea p
ig-F
PIVY
SIIF
VLGI
IAN
GYVL
W--
--FM
VNLT
VADL
LFLI
TLPL
WIV
-N
LAGC
LFFI
NTY
CSVA
FLGV
I---
--IAL
SLVI
WVA
IVAA
ASYF
LVM
--IH
ICIV
LGFF
IVFL
LILF
CNL-
----
--RAL
WM
VCTV
LAVF
VICF
VPHH
-----
QVT
LCLL
STN
CVLD
PVIY
CFL-
--He
dgeh
og-F
PIFY
SIIF
ALGV
IAN
SYVL
W--
--FM
VNLT
LADL
LFLV
TLPL
WII-
NLA
GCFF
FIN
TYCS
VAFL
GVI--
--G
IILSL
VIW
VAIV
SAAS
YFFF
---IH
IFLV
VSFF
VVFL
IIFFC
NL-
----
--RAL
WM
VCTV
LAVF
IICFV
PHH-
----
QIT
LCLL
STN
CVLD
PIIY
CFL-
--Ho
rse
--PIV
YSIIF
VLGV
IAN
SYVL
WV-
-IFM
VNLT
VADL
LFLL
TLPL
WV-
-N
LTGC
CFFI
NTY
CSVA
FLGV
I---
--ISL
SLVI
WLA
IVAA
ASYF
LFM
--IH
IFLV
FSFF
LVFL
IILVC
NL-
----
--RAL
WM
VCTV
LAVF
IICFV
PHH-
----
QVT
LCLL
STN
CVLD
PIIY
CFL-
--M
edak
a-F
SVVY
SIFF
VVGL
LAN
VYVL
F--
--YM
TNLT
IADL
LFVC
ALPF
WIG
---T
GSFF
FIN
TYGT
ILFL
GAIS
L-RG
IIVSV
LIW
LCLV
SSAI
PSL-
----H
FLIIG
LFFV
VFFL
VMVC
NLL
----
-----Q
MLL
AVVG
VFLL
CFLP
HHVV
Q--
QIT
LVLM
GLN
CILD
PVIY
YFA-
--M
onke
y-F
PIVY
SIIF
VLGV
IAN
GYVL
W--
--FM
VNLT
MAD
MLF
LITL
PLW
IV-
NLA
GCLF
FIN
TYCS
VAFL
GVI--
---I
SLSL
VIW
VAIV
GAAS
YFFI
L--IH
IFIV
FSFF
LVFL
IILFC
NL-
----
--RAL
WM
VCTV
LAVF
IICFV
PHH-
----
QVT
LCLL
STN
CVLD
PVIY
CFL-
--M
ouse
-FPI
VYSV
IFIL
GVVA
NGY
VLW
----F
MVN
LTM
ADLL
FLIT
LPLW
IV-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
--G
ISLS
LIIW
VSIV
ATAS
YFLA
--VV
HVFI
AFCF
FLVF
FLIF
YCN
------
--RAL
WM
VCTV
LAVF
IICFV
PHH-
----
-ITLC
LLST
NCV
LDPV
IYCF
LT--
Opo
ssum
-FPI
VYSI
IFVL
GFVS
NAY
VLW
-----
MVN
LTVA
DLLF
LFAL
PLW
IVY
NVA
GCLF
FIN
TYCS
VCFL
GVI--
--G
ILLS
TIIW
VVIV
GCAS
YFLI
----H
VFIV
LCFF
IVFL
VILV
CNVI
----
----L
WM
VCTV
MAV
FFIC
FVPH
HVV-
--Q
VTLC
LMSI
NCV
LDPI
IYCF
L---
Ora
ngut
an-F
PIVY
SIIF
VLGV
IAN
GYVL
W--
--FM
VNLT
MAD
MLF
LITL
PLW
IV-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
---I
SLSL
VIW
VAIV
GAAS
YFLI
L--IH
IFIV
FSFF
LVFL
IILFC
NL-
----
--RAL
WM
VCTV
LAVF
IICFV
PHH-
----
-VTL
CLLS
TNCV
LDPV
IYCF
LT--
Pig
-FPI
FYSI
IFVL
GVIA
NSY
VLW
-----
MLN
LTM
ADLL
FLVT
LPLW
IIYN
LAGC
FFFI
NTY
CSVS
FLAV
I---
--ISL
SLIIW
VAM
VAAA
SYFF
VL-
-IHIF
LVFS
FFLV
FLVI
LFCN
L--
-----R
ALW
MVC
TVLA
VFIIC
FVPH
H----
--IT
LCLL
STN
CVLD
PIIY
CFLT
--Pi
ka-F
PIVY
SIIF
VLGV
VAN
GYVL
W--
--FM
VNLT
VADL
LFLI
TLPL
WII-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
---I
SLSL
IIWVV
ILAI
ASYF
LIM
--IH
IFIV
FCFF
VVFL
IILVC
NL-
----
--RAL
WM
VCTV
LGVF
IICFV
PHH-
----
QVT
LCLL
STN
CVLD
PVIY
CFL-
--Pl
atyp
us---
VAYS
IIFVL
GIFA
NGY
VLW
VF--F
MVN
LTVA
DLLF
LVTM
PLW
IV-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
--G
IVLS
LVIW
VLIV
SSAL
YFLV
---IH
IFIV
ASFF
AVFL
LILV
CNL-
----
--RAL
WM
VCTV
LAVF
IICFV
PHH-
----
-VTL
CLLS
TNCV
LDPV
IYCF
LT--
Puffe
rfish
--PSA
YGVI
FILG
LLAN
LYVL
FI-
---GS
LFFI
NTY
CSVL
FLGA
ISVN
---M
TNLS
IADL
LFVC
ALPF
WIG
Y-G
IIISV
IIWVL
TISM
AIPY
LV--
---FA
IIGLF
FVVF
ALVL
VCN
LLI
------
----L
LAVV
GVFV
VCFL
PHHV
IQGA
QIT
LLLM
GLN
CILD
PVVY
CFA-
--Ra
bbit
--PIV
YSIIF
VLGV
VAN
GYVL
WV-
--FM
VNLT
VADL
LFLI
TLPL
WII-
NLA
GCLF
FIN
TYCS
VAFL
GVI--
---I
ALSL
VIW
VSIS
AAAT
YFLI
L--IH
IFIV
ASFF
IVFL
IILIC
NL-
----
--RAL
WM
VCTV
LAVF
IICFV
PHH-
----
QVT
LCLL
STN
CVLD
PVIY
CFL-
--Ra
t--P
IVYS
VIFV
LGVV
ANGY
VLW
V---F
MVN
LTVA
DLLF
LMTL
PLW
IV-
NLA
GCLF
FIN
TYCS
VAFL
GVI--
--G
ITLS
LVIW
ISIA
ATAS
YFLA
---V
HIFI
TSCF
FLVF
FLIF
YCN
M--
-----R
ALW
MVC
TVLA
VFVI
CFVP
HH---
---IT
LCLL
STN
CVLD
PVIY
CFLT
--Sh
rew
-FPI
FYSI
IFVL
GVIA
NSY
VLW
----F
MVN
LTM
ADLL
FLIT
LPLW
IV-
NLT
GCFF
FIN
TYCS
VAFL
GVI--
--G
IYLS
LVIW
MAI
VSAA
SYFF
F--
-IHIF
LVCS
FFLV
FLLI
FFCN
L--
-----R
ALW
MVC
TVLA
VFSI
CFVP
HH---
---V
TLCL
LSTN
CVLD
PIIY
CFLT
--Sq
uirr
elLF
PIIY
SIIF
VVGV
IAN
GYVL
-----F
MLN
LTVA
DLLF
LITL
PLW
II-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
---YL
SLVI
WVF
IVAA
ASYF
LILD
VIHI
FIVF
SFFL
VFLI
ILFC
K---
-----R
ALW
MVC
AVLA
VFVI
CFVP
HH---
--Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Stickleba
ck-F
PVVY
SIIF
ILGI
TAN
LYVL
F--
CIYM
INLT
IGDL
LFVT
FLPF
W---
--SGS
LFFI
NTY
SSVL
FLCA
ISV-
-GIIL
SVFI
WLF
TLAT
AILF
LI--
--HLT
IVIG
FFLV
FFVV
VVCN
FL-
------
--QM
LFSV
VGVF
VVCF
LPHH
ACQ
--Q
ITW
LLM
SLN
CILD
PVVY
FFA-
--Zebrafi
sh-IY
MTN
LTVA
DLLF
VSAL
PFW
I----P
IFYG
VVFV
LGLI
ANCY
ALYV
----
GTFF
FIN
TYGS
ILFL
TVIS
INRG
AIIS
VLIW
AFIL
SASI
PQL-
-----
----L
RMLC
AVIG
VFVI
CFLP
HHVV
---IT
HFSI
IGLF
FLVF
LMVI
ICN
---Q
ITLM
LMGI
NCL
LDPL
VYCF
A---
Sequ
ence
Dom
ain
ID
omai
n II
Dom
ain
III
Dom
ain
IVD
omai
n V
Dom
ain
VI
Dom
ain
VII
Tc00
.104
7053
5079
69.3
0---
AFLP
MM
AWLG
MW
YEVL
DMCV
L---
LLVF
LFPT
YTVA
CTM
ACIF
FTVV
AGIG
VAFL
IVLS
HMAF
LRV-
----E
VWHY
LLAF
FVM
HTLA
VSHA
V--
RFLG
ATLG
ALFG
GWVM
YTHY
G---
---FF
SWSF
LFQ
YVCC
LLVV
AIGK
--SY
FAVG
FMVF
YVCP
VILR
VFF-
--Hu
man
-FPI
VYSI
IFVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
-N
VAGC
-LFF
INTY
CSVA
FLGV
I---
--ISL
SLVI
WVA
IVGA
ASYF
LIL-
-IHIF
IVFS
FFLV
FLIIL
FCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
--V
TLCL
LSTN
CVLD
PVIY
CFLT
--Ch
icke
n--T
VFYS
IIFIL
GFVA
NCY
VLW
I---F
MLN
LTVA
DLLF
LVTM
PLW
IV-
SVAG
S-LF
FVN
TYAS
VAFL
MVI
----G
IFLS
AAIW
IITVG
SALY
YIF-
-AI
HIIIC
TLFY
VIFL
FILT
WN
---RA
LWM
VCTV
LVVF
IISFV
PHH-
----
-VTL
CLLS
MN
CVLD
PVIY
CFLT
--Ch
impa
nzee
-FPI
VYSI
IFVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
-N
VAGC
-LFF
INTY
CSVA
FLGV
I---
--ISL
SLVI
WVA
IVGA
ASYF
LIL-
-IHIF
IVFS
FFLV
FLIIL
FCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
--V
TLCL
LSTN
CVLD
PVIY
CFLT
--Co
w-F
PIFY
SIVF
VLGV
IAN
SYVL
W--
---M
VNLT
MAD
LLFL
VTLP
LWIV
YN
LAGC
-FFF
INTY
CSVA
FLAV
I---
--ILL
SLIIW
VSIV
GAAS
YFFV
L--IH
IFLV
FSFF
LVFL
IILFC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
-VTL
CLLS
TNCV
LDPI
IYCF
LT--
Elep
hant
-FPI
FYSI
IFVL
GVIA
NGY
VLW
-----
MVN
LTM
ADLL
FLVT
LPLW
IVY
NLA
GC-L
FFIN
TYCS
VAFL
GVI--
---I
TLSL
VIW
VTIV
AAAS
YFLF
M-
--HIF
IVCS
FFLV
FFIIL
VCN
LV-
RALW
MVC
TVLA
VFIIC
FVPH
H----
-Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Frog
---VT
YSIIF
VFGF
IAN
CYVL
WIF
-IFM
INLT
IADL
LFLV
TLPL
WI--
NVA
GC-F
FFLN
TYCS
VAFL
GVI--
----
CISV
AVW
ILLF
ASSL
YFLI
FP--H
FILV
GAFF
VVFL
IILTC
NLV
-RA
LWM
VVTV
LAVF
VLCF
VPHH
-----
---LC
LMST
NCM
LDPI
IYCF
LTKK
Goat
-FPI
FYSI
VFVL
GVIA
NSY
VLW
-----
MVN
LTM
ADLL
FLVT
LPLW
IVY
NLA
GC-F
FFIN
TYCS
VAFL
AVI--
--IH
IFLV
FSFF
LVFL
IILFC
NL-
---F
LLSL
IIWVS
IVGA
ASYF
FVL-
RALW
MVC
TVLA
VFVI
CFVP
HH---
---V
TLCL
LSTN
CVLD
PIIY
CFLT
--Gu
inea
pig
-FPI
VYSI
IFVL
GIIA
NGY
VLW
----F
MVN
LTVA
DLLF
LITL
PLW
IV-
NLA
GC-L
FFIN
TYCS
VAFL
GVI--
---I
ALSL
VIW
VAIV
AAAS
YFLV
M-
-IHIC
IVLG
FFIV
FLLI
LFCN
L--
RALW
MVC
TVLA
VFVI
CFVP
HH---
--Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Hedg
ehog
-FPI
FYSI
IFAL
GVIA
NSY
VLW
----F
MVN
LTLA
DLLF
LVTL
PLW
II-N
LAGC
-FFF
INTY
CSVA
FLGV
I---
-GIIL
SLVI
WVA
IVSA
ASYF
FF--
-IHIF
LVVS
FFVV
FLIIF
FCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
-Q
ITLC
LLST
NCV
LDPI
IYCF
L---
Hors
e--P
IVYS
IIFVL
GVIA
NSY
VLW
V--IF
MVN
LTVA
DLLF
LLTL
PLW
V--
NLT
GC-C
FFIN
TYCS
VAFL
GVI--
---I
SLSL
VIW
LAIV
AAAS
YFLF
M-
-IHIF
LVFS
FFLV
FLIIL
VCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
-Q
VTLC
LLST
NCV
LDPI
IYCF
L---
Med
aka
-FSV
VYSI
FFVV
GLLA
NVY
VLF-
---Y
MTN
LTIA
DLLF
VCAL
PFW
IG-
--TGS
-FFF
INTY
GTIL
FLGA
ISL-
RGIIV
SVLI
WLC
LVSS
AIPS
L---
--HFL
IIGLF
FVVF
FLVM
VCN
LL-
---Q
MLL
AVVG
VFLL
CFLP
HHVV
Q--
QIT
LVLM
GLN
CILD
PVIY
YFA-
--M
onke
y-F
PIVY
SIIF
VLGV
IAN
GYVL
W--
--FM
VNLT
MAD
MLF
LITL
PLW
IV-
NLA
GC-L
FFIN
TYCS
VAFL
GVI--
---I
SLSL
VIW
VAIV
GAAS
YFFI
L--IH
IFIV
FSFF
LVFL
IILFC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
QVT
LCLL
STN
CVLD
PVIY
CFL-
--M
ouse
-FPI
VYSV
IFIL
GVVA
NGY
VLW
----F
MVN
LTM
ADLL
FLIT
LPLW
IV-
NVA
GC-L
FFIN
TYCS
VAFL
GVI--
--G
ISLS
LIIW
VSIV
ATAS
YFLA
--VV
HVFI
AFCF
FLVF
FLIF
YCN
---RA
LWM
VCTV
LAVF
IICFV
PHH-
----
-ITLC
LLST
NCV
LDPV
IYCF
LT--
Opo
ssum
-FPI
VYSI
IFVL
GFVS
NAY
VLW
-----
MVN
LTVA
DLLF
LFAL
PLW
IVY
NVA
GC-L
FFIN
TYCS
VCFL
GVI--
--G
ILLS
TIIW
VVIV
GCAS
YFLI
----H
VFIV
LCFF
IVFL
VILV
CNVI
---L
WM
VCTV
MAV
FFIC
FVPH
HVV-
--Q
VTLC
LMSI
NCV
LDPI
IYCF
L---
Ora
ngut
an-F
PIVY
SIIF
VLGV
IAN
GYVL
W--
--FM
VNLT
MAD
MLF
LITL
PLW
IV-
NVA
GC-L
FFIN
TYCS
VAFL
GVI--
---I
SLSL
VIW
VAIV
GAAS
YFLI
L--IH
IFIV
FSFF
LVFL
IILFC
NL-
--V
TLCL
LSTN
CVLD
PVIY
CFLT
--RA
LWM
VCTV
LAVF
IICFV
PHH-
----
Pig
-FPI
FYSI
IFVL
GVIA
NSY
VLW
-----
MLN
LTM
ADLL
FLVT
LPLW
IIYN
LAGC
-FFF
INTY
CSVS
FLAV
I---
--ISL
SLIIW
VAM
VAAA
SYFF
VL-
-IHIF
LVFS
FFLV
FLVI
LFCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
--IT
LCLL
STN
CVLD
PIIY
CFLT
--Pi
ka-F
PIVY
SIIF
VLGV
VAN
GYVL
W--
--FM
VNLT
VADL
LFLI
TLPL
WII-
NVA
GC-L
FFIN
TYCS
VAFL
GVI--
---I
SLSL
IIWVV
ILAI
ASYF
LIM
--IH
IFIV
FCFF
VVFL
IILVC
NL-
-RA
LWM
VCTV
LGVF
IICFV
PHH-
----
QVT
LCLL
STN
CVLD
PVIY
CFL-
--Pl
atyp
us---
VAYS
IIFVL
GIFA
NGY
VLW
VF--F
MVN
LTVA
DLLF
LVTM
PLW
IV-
NVA
GC-L
FFIN
TYCS
VAFL
GVI--
--G
IVLS
LVIW
VLIV
SSAL
YFLV
---IH
IFIV
ASFF
AVFL
LILV
CNL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
-VTL
CLLS
TNCV
LDPV
IYCF
LT--
Puffe
rfish
--PSA
YGVI
FILG
LLAN
LYVL
FI-
---GS
-LFF
INTY
CSVL
FLGA
ISVN
---M
TNLS
IADL
LFVC
ALPF
WIG
Y-G
IIISV
IIWVL
TISM
AIPY
LV--
---FA
IIGLF
FVVF
ALVL
VCN
LLI
-----L
LAVV
GVFV
VCFL
PHHV
IQGA
QIT
LLLM
GLN
CILD
PVVY
CFA-
--Ra
bbit
--PIV
YSIIF
VLGV
VAN
GYVL
WV-
--FM
VNLT
VADL
LFLI
TLPL
WII-
NLA
GC-L
FFIN
TYCS
VAFL
GVI--
---I
ALSL
VIW
VSIS
AAAT
YFLI
L--IH
IFIV
ASFF
IVFL
IILIC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
QVT
LCLL
STN
CVLD
PVIY
CFL-
--Ra
t--P
IVYS
VIFV
LGVV
ANGY
VLW
V---F
MVN
LTVA
DLLF
LMTL
PLW
IV-
NLA
GC-L
FFIN
TYCS
VAFL
GVI--
--G
ITLS
LVIW
ISIA
ATAS
YFLA
---V
HIFI
TSCF
FLVF
FLIF
YCN
M--
RALW
MVC
TVLA
VFVI
CFVP
HH---
---IT
LCLL
STN
CVLD
PVIY
CFLT
--Sh
rew
-FPI
FYSI
IFVL
GVIA
NSY
VLW
----F
MVN
LTM
ADLL
FLIT
LPLW
IV-
NLT
GC-F
FFIN
TYCS
VAFL
GVI--
--G
IYLS
LVIW
MAI
VSAA
SYFF
F--
-IHIF
LVCS
FFLV
FLLI
FFCN
L--
RALW
MVC
TVLA
VFSI
CFVP
HH---
---V
TLCL
LSTN
CVLD
PIIY
CFLT
--Sq
uirr
elLF
PIIY
SIIF
VVGV
IAN
GYVL
-----F
MLN
LTVA
DLLF
LITL
PLW
II-N
VAGC
-LFF
INTY
CSVA
FLGV
I---
---YL
SLVI
WVF
IVAA
ASYF
LILD
VIHI
FIVF
SFFL
VFLI
ILFC
K---
RALW
MVC
AVLA
VFVI
CFVP
HH---
--Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Stickleba
ck-F
PVVY
SIIF
ILGI
TAN
LYVL
F--
CIYM
INLT
IGDL
LFVT
FLPF
W---
--SGS
-LFF
INTY
SSVL
FLCA
ISV-
-GIIL
SVFI
WLF
TLAT
AILF
LI--
--HLT
IVIG
FFLV
FFVV
VVCN
FL-
---Q
MLF
SVVG
VFVV
CFLP
HHAC
Q--
QIT
WLL
MSL
NCI
LDPV
VYFF
A---
Zebrafi
sh--P
IFYG
VVFV
LGLI
ANCY
ALYV
--IY
MTN
LTVA
DLLF
VSAL
PFW
I-----
GT-F
FFIN
TYGS
ILFL
TVIS
INRG
AIIS
VLIW
AFIL
SASI
PQL-
--IT
HFSI
IGLF
FLVF
LMVI
ICN
-----L
RMLC
AVIG
VFVI
CFLP
HHVV
---Q
ITLM
LMGI
NCL
LDPL
VYCF
A---
15Supplementary data Search for PAF receptor in T. cruzi • Daniel Fábio Kawano et al.
Sequ
ence
Dom
ain
ID
omai
n II
Dom
ain
III
Dom
ain
IVD
omai
n V
Dom
ain
VI
Dom
ain
VII
Tc00
.104
7053
5110
01.5
0-G
SADV
GILL
CCM
VSVF
CRVK
V--
--PLT
QRV
MVG
VGW
LSVI
SNCL
T----
SQVL
YAFF
FCIC
DMVL
HVM
EG---
--RRA
LFIV
VLN
TAM
LFFL
VIFH
--YLA
CCGT
LY---
--VLG
CDVG
VLLQ
L---
-ALY
VTQ
LVFS
FGCS
ALYF
FML-
-GT
VGLG
LLDI
ARIL
LFGS
ILW---
---Hu
man
-FPI
VYSI
IFVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLVI
WVA
IVGA
ASYF
LIL-
---IH
IFIV
FSFF
LVFL
IILFC
NL-
-----
RALW
MVC
TVLA
VFII-
CFVP
HH---
----V
TLCL
LSTN
CVLD
--PVI
YCFL
T--
Chic
ken
--TVF
YSIIF
ILGF
VAN
CYVL
WI-
--FM
LNLT
VADL
LFLV
TMPL
WIV
-SV
AGSL
FFVN
TYAS
VAFL
MVI
----G
IFLS
AAIW
IITVG
SALY
YIF-
---AI
HIIIC
TLFY
VIFL
FILT
WN
------
-RA
LWM
VCTV
LVVF
II-SF
VPHH
-----
--VTL
CLLS
MN
CVLD
--PVI
YCFL
T--
Chim
panz
ee-F
PIVY
SIIF
VLGV
IAN
GYVL
W--
--FM
VNLT
MAD
MLF
LITL
PLW
IV-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
---I
SLSL
VIW
VAIV
GAAS
YFLI
L---
-IHIF
IVFS
FFLV
FLIIL
FCN
L----
--RA
LWM
VCTV
LAVF
II-CF
VPHH
-----
--VTL
CLLS
TNCV
LD--P
VIYC
FLT-
-Co
w-F
PIFY
SIVF
VLGV
IAN
SYVL
W--
---M
VNLT
MAD
LLFL
VTLP
LWIV
YN
LAGC
FFFI
NTY
CSVA
FLAV
I---
--ILL
SLIIW
VSIV
GAAS
YFFV
L---
-IHIF
LVFS
FFLV
FLIIL
FCN
L----
----V
TLCL
LSTN
CVLD
--PIIY
CFLT
--RA
LWM
VCTV
LAVF
II-CF
VPHH
-----
Elep
hant
-FPI
FYSI
IFVL
GVIA
NGY
VLW
-----
MVN
LTM
ADLL
FLVT
LPLW
IVY
NLA
GCLF
FIN
TYCS
VAFL
GVI--
---I
TLSL
VIW
VTIV
AAAS
YFLF
M---
--HIF
IVCS
FFLV
FFIIL
VCN
LV---
--RA
LWM
VCTV
LAVF
II-CF
VPHH
-----
-QVT
LCLL
STN
CVLD
--PVI
YCFL
---Fr
og---
VTYS
IIFVF
GFIA
NCY
VLW
IF-IF
MIN
LTIA
DLLF
LVTL
PLW
I--N
VAGC
FFFL
NTY
CSVA
FLGV
I---
---CI
SVAV
WIL
LFAS
SLYF
LIFP
----H
FILV
GAFF
VVFL
IILTC
NLV
-----
RALW
MVV
TVLA
VFVL
-CFV
PHH-
----
----L
CLM
STN
CMLD
--PIIY
CFLT
KKGo
at-F
PIFY
SIVF
VLGV
IAN
SYVL
W--
NLA
GCFF
FIN
TYCS
VAFL
AVI--
----
MVN
LTM
ADLL
FLVT
LPLW
IVY
--FLL
SLIIW
VSIV
GAAS
YFFV
L---
-IHIF
LVFS
FFLV
FLIIL
FCN
L----
--RA
LWM
VCTV
LAVF
VI-C
FVPH
H----
---V
TLCL
LSTN
CVLD
--PIIY
CFLT
--Gu
inea
pig
-FPI
VYSI
IFVL
GIIA
NGY
VLW
----F
MVN
LTVA
DLLF
LITL
PLW
IV-
NLA
GCLF
FIN
TYCS
VAFL
GVI--
---I
ALSL
VIW
VAIV
AAAS
YFLV
M---
-IHIC
IVLG
FFIV
FLLI
LFCN
L----
--RA
LWM
VCTV
LAVF
VI-C
FVPH
H----
--Q
VTLC
LLST
NCV
LD--P
VIYC
FL---
Hedg
ehog
-FPI
FYSI
IFAL
GVIA
NSY
VLW
----F
MVN
LTLA
DLLF
LVTL
PLW
II-N
LAGC
FFFI
NTY
CSVA
FLGV
I---
-GIIL
SLVI
WVA
IVSA
ASYF
FF---
--IH
IFLV
VSFF
VVFL
IIFFC
NL-
-----
RALW
MVC
TVLA
VFII-
CFVP
HH---
---Q
ITLC
LLST
NCV
LD--P
IIYCF
L---
Hors
e--P
IVYS
IIFVL
GVIA
NSY
VLW
V--IF
MVN
LTVA
DLLF
LLTL
PLW
V--
NLT
GCCF
FIN
TYCS
VAFL
GVI--
---I
SLSL
VIW
LAIV
AAAS
YFLF
M---
-IHIF
LVFS
FFLV
FLIIL
VCN
L----
--RA
LWM
VCTV
LAVF
II-CF
VPHH
-----
-QVT
LCLL
STN
CVLD
--PIIY
CFL-
--M
edak
a-F
SVVY
SIFF
VVGL
LAN
VYVL
F--
--YM
TNLT
IADL
LFVC
ALPF
WIG
---T
GSFF
FIN
TYGT
ILFL
GAIS
L-RG
IIVSV
LIW
LCLV
SSAI
PSL-
----
--HFL
IIGLF
FVVF
FLVM
VCN
LL---
-----
QM
LLAV
VGVF
LL-C
FLPH
HVVQ
---Q
ITLV
LMGL
NCI
LD--P
VIYY
FA---
Mon
key
-FPI
VYSI
IFVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
-N
LAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLVI
WVA
IVGA
ASYF
FIL-
---IH
IFIV
FSFF
LVFL
IILFC
NL-
-----
RALW
MVC
TVLA
VFII-
CFVP
HH---
---Q
VTLC
LLST
NCV
LD--P
VIYC
FL---
Mou
se-F
PIVY
SVIF
ILGV
VAN
GYVL
W--
--FM
VNLT
MAD
LLFL
ITLP
LWIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
-GIS
LSLI
IWVS
IVAT
ASYF
LA---
-VV
HVFI
AFCF
FLVF
FLIF
YCN
------
-RA
LWM
VCTV
LAVF
II-CF
VPHH
-----
--ITL
CLLS
TNCV
LD--P
VIYC
FLT-
-O
poss
um-F
PIVY
SIIF
VLGF
VSN
AYVL
W--
---M
VNLT
VADL
LFLF
ALPL
WIV
YN
VAGC
LFFI
NTY
CSVC
FLGV
I---
-GIL
LSTI
IWVV
IVGC
ASYF
LI---
---H
VFIV
LCFF
IVFL
VILV
CNVI
-----
--LW
MVC
TVM
AVFF
I-CFV
PHHV
V---
-QVT
LCLM
SIN
CVLD
--PIIY
CFL-
--O
rang
utan
-FPI
VYSI
IFVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLVI
WVA
IVGA
ASYF
LIL-
---IH
IFIV
FSFF
LVFL
IILFC
NL-
-----
--VTL
CLLS
TNCV
LD--P
VIYC
FLT-
-RA
LWM
VCTV
LAVF
II-CF
VPHH
-----
Pig
-FPI
FYSI
IFVL
GVIA
NSY
VLW
-----
MLN
LTM
ADLL
FLVT
LPLW
IIYN
LAGC
FFFI
NTY
CSVS
FLAV
I---
-IHIF
LVFS
FFLV
FLVI
LFCN
L----
----I
SLSL
IIWVA
MVA
AASY
FFVL
---RA
LWM
VCTV
LAVF
II-CF
VPHH
-----
--ITL
CLLS
TNCV
LD--P
IIYCF
LT--
Pika
-FPI
VYSI
IFVL
GVVA
NGY
VLW
----F
MVN
LTVA
DLLF
LITL
PLW
II-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLIIW
VVIL
AIAS
YFLI
M---
-IHIF
IVFC
FFVV
FLIIL
VCN
L----
--RA
LWM
VCTV
LGVF
II-CF
VPHH
-----
-QVT
LCLL
STN
CVLD
--PVI
YCFL
---Pl
atyp
us---
VAYS
IIFVL
GIFA
NGY
VLW
VF--F
MVN
LTVA
DLLF
LVTM
PLW
IV-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
--G
IVLS
LVIW
VLIV
SSAL
YFLV
----
-IHIF
IVAS
FFAV
FLLI
LVCN
L----
--RA
LWM
VCTV
LAVF
II-CF
VPHH
-----
--VTL
CLLS
TNCV
LD--P
VIYC
FLT-
-Pu
fferfish
--PSA
YGVI
FILG
LLAN
LYVL
FI-
---GS
LFFI
NTY
CSVL
FLGA
ISVN
---M
TNLS
IADL
LFVC
ALPF
WIG
Y-G
IIISV
IIWVL
TISM
AIPY
LV---
----
FAIIG
LFFV
VFAL
VLVC
NLL
I----
-----L
LAVV
GVFV
V-CF
LPHH
VIQ
GA-Q
ITLL
LMGL
NCI
LD--P
VVYC
FA---
Rabb
it--P
IVYS
IIFVL
GVVA
NGY
VLW
V---F
MVN
LTVA
DLLF
LITL
PLW
II-N
LAGC
LFFI
NTY
CSVA
FLGV
I---
--IAL
SLVI
WVS
ISAA
ATYF
LIL-
---IH
IFIV
ASFF
IVFL
IILIC
NL-
-----
RALW
MVC
TVLA
VFII-
CFVP
HH---
---Q
VTLC
LLST
NCV
LD--P
VIYC
FL---
Rat
--PIV
YSVI
FVLG
VVAN
GYVL
WV-
--FM
VNLT
VADL
LFLM
TLPL
WIV
-N
LAGC
LFFI
NTY
CSVA
FLGV
I---
-GIT
LSLV
IWIS
IAAT
ASYF
LA---
--V
HIFI
TSCF
FLVF
FLIF
YCN
M---
---RA
LWM
VCTV
LAVF
VI-C
FVPH
H----
---I
TLCL
LSTN
CVLD
--PVI
YCFL
T--
Shre
w-F
PIFY
SIIF
VLGV
IAN
SYVL
W--
--FM
VNLT
MAD
LLFL
ITLP
LWIV
-N
LTGC
FFFI
NTY
CSVA
FLGV
I---
-GIY
LSLV
IWM
AIVS
AASY
FFF-
----IH
IFLV
CSFF
LVFL
LIFF
CNL-
-----
RALW
MVC
TVLA
VFSI
-CFV
PHH-
----
--VTL
CLLS
TNCV
LD--P
IIYCF
LT--
Squi
rrel
LFPI
IYSI
IFVV
GVIA
NGY
VL---
--FM
LNLT
VADL
LFLI
TLPL
WII-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
----
YLSL
VIW
VFIV
AAAS
YFLI
LD--
VIHI
FIVF
SFFL
VFLI
ILFC
K----
---RA
LWM
VCAV
LAVF
VI-C
FVPH
H----
--Q
VTLC
LLST
NCV
LD--P
VIYC
FL---
Stickleba
ck-F
PVVY
SIIF
ILGI
TAN
LYVL
F--
CIYM
INLT
IGDL
LFVT
FLPF
W---
--SGS
LFFI
NTY
SSVL
FLCA
ISV-
-GIIL
SVFI
WLF
TLAT
AILF
LI---
---H
LTIV
IGFF
LVFF
VVVV
CNFL
-----
---Q
MLF
SVVG
VFVV
-CFL
PHHA
CQ--
-QIT
WLL
MSL
NCI
LD--P
VVYF
FA---
Zebrafi
sh-IY
MTN
LTVA
DLLF
VSAL
PFW
I----P
IFYG
VVFV
LGLI
ANCY
ALYV
----
GTFF
FIN
TYGS
ILFL
TVIS
INRG
AIIS
VLIW
AFIL
SASI
PQL-
----
--LRM
LCAV
IGVF
VI-C
FLPH
HVV-
--IT
HFSI
IGLF
FLVF
LMVI
ICN
------
--Q
ITLM
LMGI
NCL
LD--P
LVYC
FA---
Sequ
ence
Dom
ain
ID
omai
n II
Dom
ain
III
Dom
ain
IVD
omai
n V
Dom
ain
VI
Dom
ain
VII
Tc00
.104
7053
5076
81.5
0-S
DLSV
TLLW
NLM
IGAV
KYVV
R--
------
----D
ARLL
WPV
PALV
VLGC
LAGT
KTT
AWCD
AFLL
VLVI
MCW
SSRV
---RA
FTLV
SGCL
ALLS
AALQ
WSF
------
LWSA
TLSI
QIL
QAL
IFIG
LHF
TGLF
VRCV
GSAA
SFLA
ALN
YC---
-----
TTFM
KLAV
VTH-
--FCF
MM
NEV
LIHu
man
-FPI
VYSI
IFVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
------
--N
VAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLVI
WVA
IVGA
ASYF
LIL-
-IHIF
IVFS
FFLV
FLIIL
FCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
--V
TLCL
LSTN
CVLD
PVIY
CFLT
-----
Chic
ken
--TVF
YSIIF
ILGF
VAN
CYVL
WI-
--FM
LNLT
VADL
LFLV
TMPL
WIV
------
--SV
AGSL
FFVN
TYAS
VAFL
MVI
----G
IFLS
AAIW
IITVG
SALY
YIF-
-AI
HIIIC
TLFY
VIFL
FILT
WN
---RA
LWM
VCTV
LVVF
IISFV
PHH-
----
-VTL
CLLS
MN
CVLD
PVIY
CFLT
-----
Chim
panz
ee-F
PIVY
SIIF
VLGV
IAN
GYVL
W--
--FM
VNLT
MAD
MLF
LITL
PLW
IV---
-----
NVA
GCLF
FIN
TYCS
VAFL
GVI--
---I
SLSL
VIW
VAIV
GAAS
YFLI
L--IH
IFIV
FSFF
LVFL
IILFC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
-VTL
CLLS
TNCV
LDPV
IYCF
LT---
--Co
w-F
PIFY
SIVF
VLGV
IAN
SYVL
W--
---M
VNLT
MAD
LLFL
VTLP
LWIV
Y----
---N
LAGC
FFFI
NTY
CSVA
FLAV
I---
--ILL
SLIIW
VSIV
GAAS
YFFV
L--IH
IFLV
FSFF
LVFL
IILFC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
-VTL
CLLS
TNCV
LDPI
IYCF
LT---
--El
epha
nt-F
PIFY
SIIF
VLGV
IAN
GYVL
W--
---M
VNLT
MAD
LLFL
VTLP
LWIV
Y----
---N
LAGC
LFFI
NTY
CSVA
FLGV
I---
--ITL
SLVI
WVT
IVAA
ASYF
LFM
---H
IFIV
CSFF
LVFF
IILVC
NLV
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
QVT
LCLL
STN
CVLD
PVIY
CFL-
-----
Frog
---VT
YSIIF
VFGF
IAN
CYVL
WIF
-IFM
INLT
IADL
LFLV
TLPL
WI--
------
-N
VAGC
FFFL
NTY
CSVA
FLGV
I---
--HFI
LVGA
FFVV
FLIIL
TCN
LV-
---CI
SVAV
WIL
LFAS
SLYF
LIFP
RALW
MVV
TVLA
VFVL
CFVP
HH---
-----
LCLM
STN
CMLD
PIIY
CFLT
KK---
Goat
-FPI
FYSI
VFVL
GVIA
NSY
VLW
-----
MVN
LTM
ADLL
FLVT
LPLW
IVY-
------
NLA
GCFF
FIN
TYCS
VAFL
AVI--
---F
LLSL
IIWVS
IVGA
ASYF
FVL-
-IHIF
LVFS
FFLV
FLIIL
FCN
L--
RALW
MVC
TVLA
VFVI
CFVP
HH---
---V
TLCL
LSTN
CVLD
PIIY
CFLT
-----
Guin
ea p
ig-F
PIVY
SIIF
VLGI
IAN
GYVL
W--
--FM
VNLT
VADL
LFLI
TLPL
WIV
------
--N
LAGC
LFFI
NTY
CSVA
FLGV
I---
--IAL
SLVI
WVA
IVAA
ASYF
LVM
--IH
ICIV
LGFF
IVFL
LILF
CNL-
-RA
LWM
VCTV
LAVF
VICF
VPHH
-----
QVT
LCLL
STN
CVLD
PVIY
CFL-
-----
Hedg
ehog
-FPI
FYSI
IFAL
GVIA
NSY
VLW
----F
MVN
LTLA
DLLF
LVTL
PLW
II----
----
NLA
GCFF
FIN
TYCS
VAFL
GVI--
--G
IILSL
VIW
VAIV
SAAS
YFFF
---IH
IFLV
VSFF
VVFL
IIFFC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
QIT
LCLL
STN
CVLD
PIIY
CFL-
-----
Hors
e--P
IVYS
IIFVL
GVIA
NSY
VLW
V--IF
MVN
LTVA
DLLF
LLTL
PLW
V----
-----
NLT
GCCF
FIN
TYCS
VAFL
GVI--
---I
SLSL
VIW
LAIV
AAAS
YFLF
M-
-IHIF
LVFS
FFLV
FLIIL
VCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
-Q
VTLC
LLST
NCV
LDPI
IYCF
L----
--M
edak
a-F
SVVY
SIFF
VVGL
LAN
VYVL
F--
--YM
TNLT
IADL
LFVC
ALPF
WIG
------
----T
GSFF
FIN
TYGT
ILFL
GAIS
L-RG
IIVSV
LIW
LCLV
SSAI
PSL-
----H
FLIIG
LFFV
VFFL
VMVC
NLL
----
QM
LLAV
VGVF
LLCF
LPHH
VVQ
--Q
ITLV
LMGL
NCI
LDPV
IYYF
A----
--M
onke
y-F
PIVY
SIIF
VLGV
IAN
GYVL
W--
--FM
VNLT
MAD
MLF
LITL
PLW
IV---
-----
NLA
GCLF
FIN
TYCS
VAFL
GVI--
---I
SLSL
VIW
VAIV
GAAS
YFFI
L--IH
IFIV
FSFF
LVFL
IILFC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
QVT
LCLL
STN
CVLD
PVIY
CFL-
-----
Mou
se-F
PIVY
SVIF
ILGV
VAN
GYVL
W--
--FM
VNLT
MAD
LLFL
ITLP
LWIV
------
--N
VAGC
LFFI
NTY
CSVA
FLGV
I---
-GIS
LSLI
IWVS
IVAT
ASYF
LA--
VVHV
FIAF
CFFL
VFFL
IFYC
N---
RALW
MVC
TVLA
VFIIC
FVPH
H----
--IT
LCLL
STN
CVLD
PVIY
CFLT
-----
Opo
ssum
-FPI
VYSI
IFVL
GFVS
NAY
VLW
-----
MVN
LTVA
DLLF
LFAL
PLW
IVY-
------
NVA
GCLF
FIN
TYCS
VCFL
GVI--
--G
ILLS
TIIW
VVIV
GCAS
YFLI
----H
VFIV
LCFF
IVFL
VILV
CNVI
---L
WM
VCTV
MAV
FFIC
FVPH
HVV-
--Q
VTLC
LMSI
NCV
LDPI
IYCF
L----
--O
rang
utan
-FPI
VYSI
IFVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
------
--N
VAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLVI
WVA
IVGA
ASYF
LIL-
-IHIF
IVFS
FFLV
FLIIL
FCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
--V
TLCL
LSTN
CVLD
PVIY
CFLT
-----
Pig
-FPI
FYSI
IFVL
GVIA
NSY
VLW
-----
MLN
LTM
ADLL
FLVT
LPLW
IIY---
----
NLA
GCFF
FIN
TYCS
VSFL
AVI--
---I
SLSL
IIWVA
MVA
AASY
FFVL
--IH
IFLV
FSFF
LVFL
VILF
CNL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
-ITLC
LLST
NCV
LDPI
IYCF
LT---
--Pi
ka-F
PIVY
SIIF
VLGV
VAN
GYVL
W--
--FM
VNLT
VADL
LFLI
TLPL
WII-
------
-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLIIW
VVIL
AIAS
YFLI
M-
-IHIF
IVFC
FFVV
FLIIL
VCN
L--
RALW
MVC
TVLG
VFIIC
FVPH
H----
-Q
VTLC
LLST
NCV
LDPV
IYCF
L----
--Pl
atyp
us---
VAYS
IIFVL
GIFA
NGY
VLW
VF--F
MVN
LTVA
DLLF
LVTM
PLW
IV---
-----
NVA
GCLF
FIN
TYCS
VAFL
GVI--
--G
IVLS
LVIW
VLIV
SSAL
YFLV
---IH
IFIV
ASFF
AVFL
LILV
CNL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
-VTL
CLLS
TNCV
LDPV
IYCF
LT---
--Pu
fferfish
--PSA
YGVI
FILG
LLAN
LYVL
FI-
---M
TNLS
IADL
LFVC
ALPF
WIG
Y----
------
GSLF
FIN
TYCS
VLFL
GAIS
VN-G
IIISV
IIWVL
TISM
AIPY
LV--
---FA
IIGLF
FVVF
ALVL
VCN
LLI
-----L
LAVV
GVFV
VCFL
PHHV
IQGA
QIT
LLLM
GLN
CILD
PVVY
CFA-
-----
Rabb
it--P
IVYS
IIFVL
GVVA
NGY
VLW
V---F
MVN
LTVA
DLLF
LITL
PLW
II----
----
NLA
GCLF
FIN
TYCS
VAFL
GVI--
---I
ALSL
VIW
VSIS
AAAT
YFLI
L--IH
IFIV
ASFF
IVFL
IILIC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
QVT
LCLL
STN
CVLD
PVIY
CFL-
-----
Rat
--PIV
YSVI
FVLG
VVAN
GYVL
WV-
--FM
VNLT
VADL
LFLM
TLPL
WIV
------
--N
LAGC
LFFI
NTY
CSVA
FLGV
I---
-GIT
LSLV
IWIS
IAAT
ASYF
LA--
-VHI
FITS
CFFL
VFFL
IFYC
NM
--RA
LWM
VCTV
LAVF
VICF
VPHH
-----
-ITLC
LLST
NCV
LDPV
IYCF
LT---
--Sh
rew
-FPI
FYSI
IFVL
GVIA
NSY
VLW
----F
MVN
LTM
ADLL
FLIT
LPLW
IV---
-----
NLT
GCFF
FIN
TYCS
VAFL
GVI--
--G
IYLS
LVIW
MAI
VSAA
SYFF
F--
-IHIF
LVCS
FFLV
FLLI
FFCN
L--
RALW
MVC
TVLA
VFSI
CFVP
HH---
---V
TLCL
LSTN
CVLD
PIIY
CFLT
-----
Squi
rrel
LFPI
IYSI
IFVV
GVIA
NGY
VL---
--FM
LNLT
VADL
LFLI
TLPL
WII-
------
-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
---YL
SLVI
WVF
IVAA
ASYF
LILD
VIHI
FIVF
SFFL
VFLI
ILFC
K---
QVT
LCLL
STN
CVLD
PVIY
CFL-
-----
RALW
MVC
AVLA
VFVI
CFVP
HH---
--Sti
ckleba
ck-F
PVVY
SIIF
ILGI
TAN
LYVL
F--
CIYM
INLT
IGDL
LFVT
FLPF
W---
------
---S
GSLF
FIN
TYSS
VLFL
CAIS
V--G
IILSV
FIW
LFTL
ATAI
LFLI
----H
LTIV
IGFF
LVFF
VVVV
CNFL
----
QM
LFSV
VGVF
VVCF
LPHH
ACQ
--Q
ITW
LLM
SLN
CILD
PVVY
FFA-
-----
Zebrafi
sh--P
IFYG
VVFV
LGLI
ANCY
ALYV
--IY
MTN
LTVA
DLLF
VSAL
PFW
I-----
----
---GT
FFFI
NTY
GSIL
FLTV
ISIN
RGAI
ISVL
IWAF
ILSA
SIPQ
L---
ITHF
SIIG
LFFL
VFLM
VIIC
N---
--LRM
LCAV
IGVF
VICF
LPHH
VV---
QIT
LMLM
GIN
CLLD
PLVY
CFA-
-----
16Supplementary data Search for PAF receptor in T. cruzi • Daniel Fábio Kawano et al.
Sequ
ence
Dom
ain
ID
omai
n II
Dom
ain
III
Dom
ain
IVD
omai
n V
Dom
ain
VI
Dom
ain
VII
Tc00
.104
7053
5068
25.2
4-F
NVW
SH---
---AL
AACF
VLYL
ALFP
PCT
CLVF
FFIF
ICSV
VYHL
FMP-
----
LSCD
VFGV
VICI
TGTS
WSL
VY---
VGM
GALL
LSTL
FVLY
GAVF
NA-
-----
FKAI
GFHS
VLRL
LILL
WVE
LP---
AVHC
HAM
SFFF
LALG
AVIN
AL--
GFW
HYCI
ILST
TMM
--LLG
CYYD
--Hu
man
-FPI
VYSI
IFVL
GVIA
NGY
VLW
------
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLVI
WVA
IVGA
ASYF
LIL-
-IHIF
IVFS
FFLV
FLIIL
FCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
---V
TLCL
LSTN
CVLD
PVIY
CFLT
--Ch
icke
n--T
VFYS
IIFIL
GFVA
NCY
VLW
I-----
----F
MLN
LTVA
DLLF
LVTM
PLW
IV-
SVAG
SLFF
VNTY
ASVA
FLM
VI---
-GIF
LSAA
IWIIT
VGSA
LYYI
F--
AIHI
IICTL
FYVI
FLFI
LTW
N---
RALW
MVC
TVLV
VFIIS
FVPH
H----
---V
TLCL
LSM
NCV
LDPV
IYCF
LT--
Chim
panz
ee-F
PIVY
SIIF
VLGV
IAN
GYVL
W---
------
-FM
VNLT
MAD
MLF
LITL
PLW
IV-
--ISL
SLVI
WVA
IVGA
ASYF
LIL-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
--IH
IFIV
FSFF
LVFL
IILFC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
--VTL
CLLS
TNCV
LDPV
IYCF
LT--
Cow
-FPI
FYSI
VFVL
GVIA
NSY
VLW
------
-----M
VNLT
MAD
LLFL
VTLP
LWIV
YN
LAGC
FFFI
NTY
CSVA
FLAV
I---
--ILL
SLIIW
VSIV
GAAS
YFFV
L--IH
IFLV
FSFF
LVFL
IILFC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
--VTL
CLLS
TNCV
LDPI
IYCF
LT--
Elep
hant
-FPI
FYSI
IFVL
GVIA
NGY
VLW
------
-----M
VNLT
MAD
LLFL
VTLP
LWIV
Y--I
TLSL
VIW
VTIV
AAAS
YFLF
M-
NLA
GCLF
FIN
TYCS
VAFL
GVI--
---H
IFIV
CSFF
LVFF
IILVC
NLV
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
-QVT
LCLL
STN
CVLD
PVIY
CFL-
--Fr
og---
VTYS
IIFVF
GFIA
NCY
VLW
IF---
----
IFM
INLT
IADL
LFLV
TLPL
WI--
NVA
GCFF
FLN
TYCS
VAFL
GVI--
----
CISV
AVW
ILLF
ASSL
YFLI
FP--H
FILV
GAFF
VVFL
IILTC
NLV
-RA
LWM
VVTV
LAVF
VLCF
VPHH
-----
----L
CLM
STN
CMLD
PIIY
CFLT
KKGo
at-F
PIFY
SIVF
VLGV
IAN
SYVL
W---
------
--MVN
LTM
ADLL
FLVT
LPLW
IVY
NLA
GCFF
FIN
TYCS
VAFL
AVI--
---F
LLSL
IIWVS
IVGA
ASYF
FVL-
-IHIF
LVFS
FFLV
FLIIL
FCN
L--
RALW
MVC
TVLA
VFVI
CFVP
HH---
----V
TLCL
LSTN
CVLD
PIIY
CFLT
--Gu
inea
pig
-FPI
VYSI
IFVL
GIIA
NGY
VLW
------
----F
MVN
LTVA
DLLF
LITL
PLW
IV-
NLA
GCLF
FIN
TYCS
VAFL
GVI--
---I
ALSL
VIW
VAIV
AAAS
YFLV
M-
-IHIC
IVLG
FFIV
FLLI
LFCN
L--
RALW
MVC
TVLA
VFVI
CFVP
HH---
---Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Hedg
ehog
-FPI
FYSI
IFAL
GVIA
NSY
VLW
------
----F
MVN
LTLA
DLLF
LVTL
PLW
II-N
LAGC
FFFI
NTY
CSVA
FLGV
I---
-GIIL
SLVI
WVA
IVSA
ASYF
FF--
-IHIF
LVVS
FFVV
FLIIF
FCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
--Q
ITLC
LLST
NCV
LDPI
IYCF
L---
Hors
e--P
IVYS
IIFVL
GVIA
NSY
VLW
V----
----
IFM
VNLT
VADL
LFLL
TLPL
WV-
-N
LTGC
CFFI
NTY
CSVA
FLGV
I---
--ISL
SLVI
WLA
IVAA
ASYF
LFM
--IH
IFLV
FSFF
LVFL
IILVC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
-QVT
LCLL
STN
CVLD
PIIY
CFL-
--M
edak
a-F
SVVY
SIFF
VVGL
LAN
VYVL
F----
-----
-YM
TNLT
IADL
LFVC
ALPF
WIG
---T
GSFF
FIN
TYGT
ILFL
GAIS
L-RG
IIVSV
LIW
LCLV
SSAI
PSL-
----H
FLIIG
LFFV
VFFL
VMVC
NLL
----
QM
LLAV
VGVF
LLCF
LPHH
VVQ
---Q
ITLV
LMGL
NCI
LDPV
IYYF
A---
Mon
key
-FPI
VYSI
IFVL
GVIA
NGY
VLW
------
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
-N
LAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLVI
WVA
IVGA
ASYF
FIL-
-IHIF
IVFS
FFLV
FLIIL
FCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
--Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Mou
se-F
PIVY
SVIF
ILGV
VAN
GYVL
W---
------
-FM
VNLT
MAD
LLFL
ITLP
LWIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
-GIS
LSLI
IWVS
IVAT
ASYF
LA--
VVHV
FIAF
CFFL
VFFL
IFYC
N---
RALW
MVC
TVLA
VFIIC
FVPH
H----
---I
TLCL
LSTN
CVLD
PVIY
CFLT
--O
poss
um-F
PIVY
SIIF
VLGF
VSN
AYVL
W---
------
--MVN
LTVA
DLLF
LFAL
PLW
IVY
NVA
GCLF
FIN
TYCS
VCFL
GVI--
--G
ILLS
TIIW
VVIV
GCAS
YFLI
----H
VFIV
LCFF
IVFL
VILV
CNVI
---L
WM
VCTV
MAV
FFIC
FVPH
HVV-
---Q
VTLC
LMSI
NCV
LDPI
IYCF
L---
Ora
ngut
an-F
PIVY
SIIF
VLGV
IAN
GYVL
W---
------
-FM
VNLT
MAD
MLF
LITL
PLW
IV-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
---I
SLSL
VIW
VAIV
GAAS
YFLI
L--IH
IFIV
FSFF
LVFL
IILFC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
--VTL
CLLS
TNCV
LDPV
IYCF
LT--
Pig
-FPI
FYSI
IFVL
GVIA
NSY
VLW
------
-----M
LNLT
MAD
LLFL
VTLP
LWIIY
NLA
GCFF
FIN
TYCS
VSFL
AVI--
---I
SLSL
IIWVA
MVA
AASY
FFVL
--IH
IFLV
FSFF
LVFL
VILF
CNL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
--ITL
CLLS
TNCV
LDPI
IYCF
LT--
Pika
-FPI
VYSI
IFVL
GVVA
NGY
VLW
------
----F
MVN
LTVA
DLLF
LITL
PLW
II-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLIIW
VVIL
AIAS
YFLI
M-
-IHIF
IVFC
FFVV
FLIIL
VCN
L--
RALW
MVC
TVLG
VFIIC
FVPH
H----
--Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Plat
ypus
---VA
YSIIF
VLGI
FAN
GYVL
WVF
----
----F
MVN
LTVA
DLLF
LVTM
PLW
IV-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
--IH
IFIV
ASFF
AVFL
LILV
CNL-
--G
IVLS
LVIW
VLIV
SSAL
YFLV
--RA
LWM
VCTV
LAVF
IICFV
PHH-
----
--VTL
CLLS
TNCV
LDPV
IYCF
LT--
Puffe
rfish
--PSA
YGVI
FILG
LLAN
LYVL
FI---
-----
--MTN
LSIA
DLLF
VCAL
PFW
IGY
---GS
LFFI
NTY
CSVL
FLGA
ISVN
-GIII
SVIIW
VLTI
SMAI
PYLV
-----
FAIIG
LFFV
VFAL
VLVC
NLL
I---
--LLA
VVGV
FVVC
FLPH
HVIQ
GA-Q
ITLL
LMGL
NCI
LDPV
VYCF
A---
Rabb
it--P
IVYS
IIFVL
GVVA
NGY
VLW
V----
----
-FM
VNLT
VADL
LFLI
TLPL
WII-
NLA
GCLF
FIN
TYCS
VAFL
GVI--
---I
ALSL
VIW
VSIS
AAAT
YFLI
L--IH
IFIV
ASFF
IVFL
IILIC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
-QVT
LCLL
STN
CVLD
PVIY
CFL-
--Ra
t--P
IVYS
VIFV
LGVV
ANGY
VLW
V----
----
-FM
VNLT
VADL
LFLM
TLPL
WIV
-N
LAGC
LFFI
NTY
CSVA
FLGV
I---
-GIT
LSLV
IWIS
IAAT
ASYF
LA--
-VHI
FITS
CFFL
VFFL
IFYC
NM
--RA
LWM
VCTV
LAVF
VICF
VPHH
-----
--ITL
CLLS
TNCV
LDPV
IYCF
LT--
Shre
w-F
PIFY
SIIF
VLGV
IAN
SYVL
W---
------
-FM
VNLT
MAD
LLFL
ITLP
LWIV
-N
LTGC
FFFI
NTY
CSVA
FLGV
I---
-GIY
LSLV
IWM
AIVS
AASY
FFF-
--IH
IFLV
CSFF
LVFL
LIFF
CNL-
-RA
LWM
VCTV
LAVF
SICF
VPHH
-----
--VTL
CLLS
TNCV
LDPI
IYCF
LT--
Squi
rrel
LFPI
IYSI
IFVV
GVIA
NGY
VL---
----
----F
MLN
LTVA
DLLF
LITL
PLW
II-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
---YL
SLVI
WVF
IVAA
ASYF
LILD
VIHI
FIVF
SFFL
VFLI
ILFC
K---
RALW
MVC
AVLA
VFVI
CFVP
HH---
---Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Stickleba
ck-F
PVVY
SIIF
ILGI
TAN
LYVL
F----
----C
IYM
INLT
IGDL
LFVT
FLPF
W---
--SGS
LFFI
NTY
SSVL
FLCA
ISV-
-GIIL
SVFI
WLF
TLAT
AILF
LI--
--HLT
IVIG
FFLV
FFVV
VVCN
FL-
---Q
MLF
SVVG
VFVV
CFLP
HHAC
Q--
-QIT
WLL
MSL
NCI
LDPV
VYFF
A---
Zebrafi
sh--P
IFYG
VVFV
LGLI
ANCY
ALYV
-----
---IY
MTN
LTVA
DLLF
VSAL
PFW
I-----
GTFF
FIN
TYGS
ILFL
TVIS
INRG
AIIS
VLIW
AFIL
SASI
PQL-
--IT
HFSI
IGLF
FLVF
LMVI
ICN
-----L
RMLC
AVIG
VFVI
CFLP
HHVV
----Q
ITLM
LMGI
NCL
LDPL
VYCF
A---
Sequ
ence
Dom
ain
ID
omai
n II
Dom
ain
III
Dom
ain
IVD
omai
n V
Dom
ain
VI
Dom
ain
VII
Tc00
.104
7053
5115
07.6
0---
--SRV
VSVF
HSVL
VVPA
LLGG
VA-M
QG-
-LLC
ISIG
YFLY
DTAV
LVL
FHHV
VSSI
PYLI
------
YMFI
GY-Y
GLFI
LVC-
---FM
LVET
TNIS
-LF
FLW
IVFR
LIN
PTLL
LIIM
------
-AP
SVLC
AYVV
SFLC
YGVF
VIL-
-----
--SRV
VSVF
HSVL
VVPA
LLGG
VAHu
man
--FM
VNLT
MAD
MLF
LITL
PLW
IV---
NVA
GCLF
FIN
TYCS
VAFL
GVI--
---I
SLSL
VIW
VAIV
GAAS
YFLI
L--IH
IFIV
FSFF
LVFL
IILFC
NL-
--R
ALW
MVC
TVLA
VFIIC
FVPH
H----
--V
TLCL
LSTN
CVLD
PVIY
CFLT
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
---Ch
icke
n--F
MLN
LTVA
DLLF
LVTM
PLW
IV---
SVAG
SLFF
VNTY
ASVA
FLM
VI---
-GIF
LSAA
IWIIT
VGSA
LYYI
F--
AIHI
IICTL
FYVI
FLFI
LTW
N---
-RAL
WM
VCTV
LVVF
IISFV
PHH-
----
-VTL
CLLS
MN
CVLD
PVIY
CFLT
----F
MLN
LTVA
DLLF
LVTM
PLW
IV---
Chim
panz
ee--F
MVN
LTM
ADM
LFLI
TLPL
WIV
---N
VAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLVI
WVA
IVGA
ASYF
LIL-
-IHIF
IVFS
FFLV
FLIIL
FCN
L--
-RAL
WM
VCTV
LAVF
IICFV
PHH-
----
-VTL
CLLS
TNCV
LDPV
IYCF
LT--
--FM
VNLT
MAD
MLF
LITL
PLW
IV---
Cow
---M
VNLT
MAD
LLFL
VTLP
LWIV
Y--
NLA
GCFF
FIN
TYCS
VAFL
AVI--
---I
LLSL
IIWVS
IVGA
ASYF
FVL-
-IHIF
LVFS
FFLV
FLIIL
FCN
L--
-RAL
WM
VCTV
LAVF
IICFV
PHH-
----
-VTL
CLLS
TNCV
LDPI
IYCF
LT--
---M
VNLT
MAD
LLFL
VTLP
LWIV
Y--
Elep
hant
---M
VNLT
MAD
LLFL
VTLP
LWIV
Y--
NLA
GCLF
FIN
TYCS
VAFL
GVI--
---I
TLSL
VIW
VTIV
AAAS
YFLF
M-
--HIF
IVCS
FFLV
FFIIL
VCN
LV-
-RAL
WM
VCTV
LAVF
IICFV
PHH-
----
QVT
LCLL
STN
CVLD
PVIY
CFL-
-----
MVN
LTM
ADLL
FLVT
LPLW
IVY-
-Fr
og-IF
MIN
LTIA
DLLF
LVTL
PLW
I----
NVA
GCFF
FLN
TYCS
VAFL
GVI--
----
CISV
AVW
ILLF
ASSL
YFLI
FP--H
FILV
GAFF
VVFL
IILTC
NLV
--R
ALW
MVV
TVLA
VFVL
CFVP
HH---
-----
LCLM
STN
CMLD
PIIY
CFLT
KK-IF
MIN
LTIA
DLLF
LVTL
PLW
I----
Goat
---M
VNLT
MAD
LLFL
VTLP
LWIV
Y--
NLA
GCFF
FIN
TYCS
VAFL
AVI--
---F
LLSL
IIWVS
IVGA
ASYF
FVL-
-IHIF
LVFS
FFLV
FLIIL
FCN
L--
-RAL
WM
VCTV
LAVF
VICF
VPHH
-----
-VTL
CLLS
TNCV
LDPI
IYCF
LT--
---M
VNLT
MAD
LLFL
VTLP
LWIV
Y--
Guin
ea p
ig--F
MVN
LTVA
DLLF
LITL
PLW
IV---
NLA
GCLF
FIN
TYCS
VAFL
GVI--
---I
ALSL
VIW
VAIV
AAAS
YFLV
M-
-IHIC
IVLG
FFIV
FLLI
LFCN
L--
-RAL
WM
VCTV
LAVF
VICF
VPHH
-----
QVT
LCLL
STN
CVLD
PVIY
CFL-
----F
MVN
LTVA
DLLF
LITL
PLW
IV---
Hedg
ehog
--FM
VNLT
LADL
LFLV
TLPL
WII-
--N
LAGC
FFFI
NTY
CSVA
FLGV
I---
-GIIL
SLVI
WVA
IVSA
ASYF
FF--
-IHIF
LVVS
FFVV
FLIIF
FCN
L--
-RAL
WM
VCTV
LAVF
IICFV
PHH-
----
QIT
LCLL
STN
CVLD
PIIY
CFL-
----F
MVN
LTLA
DLLF
LVTL
PLW
II---
Hors
e-IF
MVN
LTVA
DLLF
LLTL
PLW
V----
NLT
GCCF
FIN
TYCS
VAFL
GVI--
---I
SLSL
VIW
LAIV
AAAS
YFLF
M-
-IHIF
LVFS
FFLV
FLIIL
VCN
L--
-RAL
WM
VCTV
LAVF
IICFV
PHH-
----
QVT
LCLL
STN
CVLD
PIIY
CFL-
---IF
MVN
LTVA
DLLF
LLTL
PLW
V----
Med
aka
--YM
TNLT
IADL
LFVC
ALPF
WIG
-----T
GSFF
FIN
TYGT
ILFL
GAIS
L-RG
IIVSV
LIW
LCLV
SSAI
PSL-
----H
FLIIG
LFFV
VFFL
VMVC
NLL
----
-QM
LLAV
VGVF
LLCF
LPHH
VVQ
--Q
ITLV
LMGL
NCI
LDPV
IYYF
A---
--YM
TNLT
IADL
LFVC
ALPF
WIG
---M
onke
y--F
MVN
LTM
ADM
LFLI
TLPL
WIV
---N
LAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLVI
WVA
IVGA
ASYF
FIL-
-IHIF
IVFS
FFLV
FLIIL
FCN
L--
-RAL
WM
VCTV
LAVF
IICFV
PHH-
----
QVT
LCLL
STN
CVLD
PVIY
CFL-
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
---M
ouse
--FM
VNLT
MAD
LLFL
ITLP
LWIV
---N
VAGC
LFFI
NTY
CSVA
FLGV
I---
-GIS
LSLI
IWVS
IVAT
ASYF
LA--
VVHV
FIAF
CFFL
VFFL
IFYC
N---
-RAL
WM
VCTV
LAVF
IICFV
PHH-
----
-ITLC
LLST
NCV
LDPV
IYCF
LT--
--FM
VNLT
MAD
LLFL
ITLP
LWIV
---O
poss
um---
MVN
LTVA
DLLF
LFAL
PLW
IVY-
-N
VAGC
LFFI
NTY
CSVC
FLGV
I---
-GIL
LSTI
IWVV
IVGC
ASYF
LI--
--HVF
IVLC
FFIV
FLVI
LVCN
VI-
---LW
MVC
TVM
AVFF
ICFV
PHHV
V---
QVT
LCLM
SIN
CVLD
PIIY
CFL-
-----
MVN
LTVA
DLLF
LFAL
PLW
IVY-
-O
rang
utan
--FM
VNLT
MAD
MLF
LITL
PLW
IV---
NVA
GCLF
FIN
TYCS
VAFL
GVI--
---I
SLSL
VIW
VAIV
GAAS
YFLI
L--IH
IFIV
FSFF
LVFL
IILFC
NL-
--R
ALW
MVC
TVLA
VFIIC
FVPH
H----
--V
TLCL
LSTN
CVLD
PVIY
CFLT
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
---Pi
g---
MLN
LTM
ADLL
FLVT
LPLW
IIY--
NLA
GCFF
FIN
TYCS
VSFL
AVI--
---I
SLSL
IIWVA
MVA
AASY
FFVL
--IH
IFLV
FSFF
LVFL
VILF
CNL-
--R
ALW
MVC
TVLA
VFIIC
FVPH
H----
--IT
LCLL
STN
CVLD
PIIY
CFLT
-----
MLN
LTM
ADLL
FLVT
LPLW
IIY--
Pika
--FM
VNLT
VADL
LFLI
TLPL
WII-
--N
VAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLIIW
VVIL
AIAS
YFLI
M-
-IHIF
IVFC
FFVV
FLIIL
VCN
L--
-RAL
WM
VCTV
LGVF
IICFV
PHH-
----
QVT
LCLL
STN
CVLD
PVIY
CFL-
----F
MVN
LTVA
DLLF
LITL
PLW
II---
Plat
ypus
--FM
VNLT
VADL
LFLV
TMPL
WIV
---N
VAGC
LFFI
NTY
CSVA
FLGV
I---
-GIV
LSLV
IWVL
IVSS
ALYF
LV--
-IHIF
IVAS
FFAV
FLLI
LVCN
L--
-RAL
WM
VCTV
LAVF
IICFV
PHH-
----
-VTL
CLLS
TNCV
LDPV
IYCF
LT--
--FM
VNLT
VADL
LFLV
TMPL
WIV
---Pu
fferfish
---M
TNLS
IADL
LFVC
ALPF
WIG
Y--
---GS
LFFI
NTY
CSVL
FLGA
ISVN
-GIII
SVIIW
VLTI
SMAI
PYLV
-----
FAIIG
LFFV
VFAL
VLVC
NLL
I---
---LL
AVVG
VFVV
CFLP
HHVI
QGA
QIT
LLLM
GLN
CILD
PVVY
CFA-
-----
MTN
LSIA
DLLF
VCAL
PFW
IGY-
-Ra
bbit
--FM
VNLT
VADL
LFLI
TLPL
WII-
--N
LAGC
LFFI
NTY
CSVA
FLGV
I---
--IAL
SLVI
WVS
ISAA
ATYF
LIL-
-IHIF
IVAS
FFIV
FLIIL
ICN
L--
-RAL
WM
VCTV
LAVF
IICFV
PHH-
----
QVT
LCLL
STN
CVLD
PVIY
CFL-
----F
MVN
LTVA
DLLF
LITL
PLW
II---
Rat
--FM
VNLT
VADL
LFLM
TLPL
WIV
---N
LAGC
LFFI
NTY
CSVA
FLGV
I---
-GIT
LSLV
IWIS
IAAT
ASYF
LA--
-VHI
FITS
CFFL
VFFL
IFYC
NM
---R
ALW
MVC
TVLA
VFVI
CFVP
HH---
---IT
LCLL
STN
CVLD
PVIY
CFLT
----F
MVN
LTVA
DLLF
LMTL
PLW
IV---
Shre
w--F
MVN
LTM
ADLL
FLIT
LPLW
IV---
NLT
GCFF
FIN
TYCS
VAFL
GVI--
--G
IYLS
LVIW
MAI
VSAA
SYFF
F--
-IHIF
LVCS
FFLV
FLLI
FFCN
L--
-RAL
WM
VCTV
LAVF
SICF
VPHH
-----
-VTL
CLLS
TNCV
LDPI
IYCF
LT--
--FM
VNLT
MAD
LLFL
ITLP
LWIV
---Sq
uirr
el--F
MLN
LTVA
DLLF
LITL
PLW
II---
NVA
GCLF
FIN
TYCS
VAFL
GVI--
----
YLSL
VIW
VFIV
AAAS
YFLI
LDVI
HIFI
VFSF
FLVF
LIIL
FCK-
---R
ALW
MVC
AVLA
VFVI
CFVP
HH---
--Q
VTLC
LLST
NCV
LDPV
IYCF
L---
--FM
LNLT
VADL
LFLI
TLPL
WII-
--Sti
ckleba
ckCI
YMIN
LTIG
DLLF
VTFL
PFW
-----
--SGS
LFFI
NTY
SSVL
FLCA
ISV-
-GIIL
SVFI
WLF
TLAT
AILF
LI--
--HLT
IVIG
FFLV
FFVV
VVCN
FL-
----Q
MLF
SVVG
VFVV
CFLP
HHAC
Q--
QIT
WLL
MSL
NCI
LDPV
VYFF
A---
CIYM
INLT
IGDL
LFVT
FLPF
W---
--Zebrafi
sh-IY
MTN
LTVA
DLLF
VSAL
PFW
I----
---GT
FFFI
NTY
GSIL
FLTV
ISIN
RGAI
ISVL
IWAF
ILSA
SIPQ
L---
ITHF
SIIG
LFFL
VFLM
VIIC
N---
---LR
MLC
AVIG
VFVI
CFLP
HHVV
---Q
ITLM
LMGI
NCL
LDPL
VYCF
A---
-IYM
TNLT
VADL
LFVS
ALPF
WI--
--
17Supplementary data Search for PAF receptor in T. cruzi • Daniel Fábio Kawano et al.
Sequ
ence
Dom
ain
ID
omai
n II
Dom
ain
III
Dom
ain
IVD
omai
n V
Dom
ain
VI
Dom
ain
VII
Tc00
.104
7053
5085
35.3
0-C
HMAD
LVIW
VYIL
ALLL
FMSH
-----
-LHY
FLLD
YCYF
HNAC
-----
------
-----L
VLFL
LWCL
VDVQ
---FL
SFFA
LVAG
SFGP
ILGA
ILV-S
YWTM
LKLH
FVM
FIAW
QIF
YH--
MFG
TGYL
PTVI
LFQ
VAQ
WLL
H----
-AL
SAW
PLAL
YLIF
FLLY
TVYN
---Hu
man
-FPI
VYSI
IFVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLVI
WVA
IVGA
ASYF
LIL-
-IHIF
IVFS
FFLV
FLIIL
FCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
--V
TLCL
LSTN
CVLD
PVIY
CFLT
--Ch
icke
n--T
VFYS
IIFIL
GFVA
NCY
VLW
I---F
MLN
LTVA
DLLF
LVTM
PLW
IV-
SVAG
SLFF
VNTY
ASVA
FLM
VI---
-GIF
LSAA
IWIIT
VGSA
LYYI
F--
AIHI
IICTL
FYVI
FLFI
LTW
N---
RALW
MVC
TVLV
VFIIS
FVPH
H----
--V
TLCL
LSM
NCV
LDPV
IYCF
LT--
Chim
panz
ee-F
PIVY
SIIF
VLGV
IAN
GYVL
W--
--FM
VNLT
MAD
MLF
LITL
PLW
IV-
NVA
GCLF
FIN
TYCS
VAFL
GVI--
---I
SLSL
VIW
VAIV
GAAS
YFLI
L--IH
IFIV
FSFF
LVFL
IILFC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
-VTL
CLLS
TNCV
LDPV
IYCF
LT--
Cow
-FPI
FYSI
VFVL
GVIA
NSY
VLW
-----
MVN
LTM
ADLL
FLVT
LPLW
IVY
NLA
GCFF
FIN
TYCS
VAFL
AVI--
---I
LLSL
IIWVS
IVGA
ASYF
FVL-
-IHIF
LVFS
FFLV
FLIIL
FCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
--V
TLCL
LSTN
CVLD
PIIY
CFLT
--El
epha
nt-F
PIFY
SIIF
VLGV
IAN
GYVL
W--
---M
VNLT
MAD
LLFL
VTLP
LWIV
YN
LAGC
LFFI
NTY
CSVA
FLGV
I---
--ITL
SLVI
WVT
IVAA
ASYF
LFM
---H
IFIV
CSFF
LVFF
IILVC
NLV
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
QVT
LCLL
STN
CVLD
PVIY
CFL-
--Fr
og---
VTYS
IIFVF
GFIA
NCY
VLW
IF-IF
MIN
LTIA
DLLF
LVTL
PLW
I--N
VAGC
FFFL
NTY
CSVA
FLGV
I---
---CI
SVAV
WIL
LFAS
SLYF
LIFP
--HFI
LVGA
FFVV
FLIIL
TCN
LV-
RALW
MVV
TVLA
VFVL
CFVP
HH---
-----
LCLM
STN
CMLD
PIIY
CFLT
KKGo
at-F
PIFY
SIVF
VLGV
IAN
SYVL
W--
---M
VNLT
MAD
LLFL
VTLP
LWIV
YN
LAGC
FFFI
NTY
CSVA
FLAV
I---
--FLL
SLIIW
VSIV
GAAS
YFFV
L--IH
IFLV
FSFF
LVFL
IILFC
NL-
-RA
LWM
VCTV
LAVF
VICF
VPHH
-----
-VTL
CLLS
TNCV
LDPI
IYCF
LT--
Guin
ea p
ig-F
PIVY
SIIF
VLGI
IAN
GYVL
W--
--FM
VNLT
VADL
LFLI
TLPL
WIV
-N
LAGC
LFFI
NTY
CSVA
FLGV
I---
--IAL
SLVI
WVA
IVAA
ASYF
LVM
--IH
ICIV
LGFF
IVFL
LILF
CNL-
-RA
LWM
VCTV
LAVF
VICF
VPHH
-----
QVT
LCLL
STN
CVLD
PVIY
CFL-
--He
dgeh
og-F
PIFY
SIIF
ALGV
IAN
SYVL
W--
--FM
VNLT
LADL
LFLV
TLPL
WII-
NLA
GCFF
FIN
TYCS
VAFL
GVI--
--G
IILSL
VIW
VAIV
SAAS
YFFF
---IH
IFLV
VSFF
VVFL
IIFFC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
QIT
LCLL
STN
CVLD
PIIY
CFL-
--Ho
rse
--PIV
YSIIF
VLGV
IAN
SYVL
WV-
-IFM
VNLT
VADL
LFLL
TLPL
WV-
-N
LTGC
CFFI
NTY
CSVA
FLGV
I---
--ISL
SLVI
WLA
IVAA
ASYF
LFM
--IH
IFLV
FSFF
LVFL
IILVC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
QVT
LCLL
STN
CVLD
PIIY
CFL-
--M
edak
a-F
SVVY
SIFF
VVGL
LAN
VYVL
F--
--YM
TNLT
IADL
LFVC
ALPF
WIG
---T
GSFF
FIN
TYGT
ILFL
GAIS
L-RG
IIVSV
LIW
LCLV
SSAI
PSL-
----H
FLIIG
LFFV
VFFL
VMVC
NLL
----
QM
LLAV
VGVF
LLCF
LPHH
VVQ
--Q
ITLV
LMGL
NCI
LDPV
IYYF
A---
Mon
key
-FPI
VYSI
IFVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
-N
LAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLVI
WVA
IVGA
ASYF
FIL-
-IHIF
IVFS
FFLV
FLIIL
FCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
-Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Mou
se-F
PIVY
SVIF
ILGV
VAN
GYVL
W--
--FM
VNLT
MAD
LLFL
ITLP
LWIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
-GIS
LSLI
IWVS
IVAT
ASYF
LA--
VVHV
FIAF
CFFL
VFFL
IFYC
N---
RALW
MVC
TVLA
VFIIC
FVPH
H----
--IT
LCLL
STN
CVLD
PVIY
CFLT
--O
poss
um-F
PIVY
SIIF
VLGF
VSN
AYVL
W--
NVA
GCLF
FIN
TYCS
VCFL
GVI--
----
MVN
LTVA
DLLF
LFAL
PLW
IVY
-GIL
LSTI
IWVV
IVGC
ASYF
LI--
--HVF
IVLC
FFIV
FLVI
LVCN
VI-
--LW
MVC
TVM
AVFF
ICFV
PHHV
V---
QVT
LCLM
SIN
CVLD
PIIY
CFL-
--O
rang
utan
-FPI
VYSI
IFVL
GVIA
NGY
VLW
----F
MVN
LTM
ADM
LFLI
TLPL
WIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLVI
WVA
IVGA
ASYF
LIL-
-IHIF
IVFS
FFLV
FLIIL
FCN
L--
RALW
MVC
TVLA
VFIIC
FVPH
H----
--V
TLCL
LSTN
CVLD
PVIY
CFLT
--Pi
g-F
PIFY
SIIF
VLGV
IAN
SYVL
W--
---M
LNLT
MAD
LLFL
VTLP
LWIIY
NLA
GCFF
FIN
TYCS
VSFL
AVI--
---I
SLSL
IIWVA
MVA
AASY
FFVL
--IH
IFLV
FSFF
LVFL
VILF
CNL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
-ITLC
LLST
NCV
LDPI
IYCF
LT--
Pika
-FPI
VYSI
IFVL
GVVA
NGY
VLW
----F
MVN
LTVA
DLLF
LITL
PLW
II-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
--ISL
SLIIW
VVIL
AIAS
YFLI
M-
-IHIF
IVFC
FFVV
FLIIL
VCN
L--
RALW
MVC
TVLG
VFIIC
FVPH
H----
-Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Plat
ypus
---VA
YSIIF
VLGI
FAN
GYVL
WVF
--FM
VNLT
VADL
LFLV
TMPL
WIV
-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
-IHIF
IVAS
FFAV
FLLI
LVCN
L--
-GIV
LSLV
IWVL
IVSS
ALYF
LV--
RALW
MVC
TVLA
VFIIC
FVPH
H----
--V
TLCL
LSTN
CVLD
PVIY
CFLT
--Pu
fferfish
--PSA
YGVI
FILG
LLAN
LYVL
FI-
---M
TNLS
IADL
LFVC
ALPF
WIG
Y---
GSLF
FIN
TYCS
VLFL
GAIS
VN-G
IIISV
IIWVL
TISM
AIPY
LV--
---FA
IIGLF
FVVF
ALVL
VCN
LLI
-----L
LAVV
GVFV
VCFL
PHHV
IQGA
QIT
LLLM
GLN
CILD
PVVY
CFA-
--Ra
bbit
--PIV
YSIIF
VLGV
VAN
GYVL
WV-
--FM
VNLT
VADL
LFLI
TLPL
WII-
NLA
GCLF
FIN
TYCS
VAFL
GVI--
---I
ALSL
VIW
VSIS
AAAT
YFLI
L--IH
IFIV
ASFF
IVFL
IILIC
NL-
-RA
LWM
VCTV
LAVF
IICFV
PHH-
----
QVT
LCLL
STN
CVLD
PVIY
CFL-
--Ra
t--P
IVYS
VIFV
LGVV
ANGY
VLW
V---F
MVN
LTVA
DLLF
LMTL
PLW
IV-
NLA
GCLF
FIN
TYCS
VAFL
GVI--
--G
ITLS
LVIW
ISIA
ATAS
YFLA
---V
HIFI
TSCF
FLVF
FLIF
YCN
M--
RALW
MVC
TVLA
VFVI
CFVP
HH---
---IT
LCLL
STN
CVLD
PVIY
CFLT
--Sh
rew
-FPI
FYSI
IFVL
GVIA
NSY
VLW
----F
MVN
LTM
ADLL
FLIT
LPLW
IV-
NLT
GCFF
FIN
TYCS
VAFL
GVI--
--G
IYLS
LVIW
MAI
VSAA
SYFF
F--
-IHIF
LVCS
FFLV
FLLI
FFCN
L--
RALW
MVC
TVLA
VFSI
CFVP
HH---
---V
TLCL
LSTN
CVLD
PIIY
CFLT
--Sq
uirr
elLF
PIIY
SIIF
VVGV
IAN
GYVL
-----F
MLN
LTVA
DLLF
LITL
PLW
II-N
VAGC
LFFI
NTY
CSVA
FLGV
I---
---YL
SLVI
WVF
IVAA
ASYF
LILD
VIHI
FIVF
SFFL
VFLI
ILFC
K---
RALW
MVC
AVLA
VFVI
CFVP
HH---
--Q
VTLC
LLST
NCV
LDPV
IYCF
L---
Stickleba
ck-F
PVVY
SIIF
ILGI
TAN
LYVL
F--
CIYM
INLT
IGDL
LFVT
FLPF
W---
--SGS
LFFI
NTY
SSVL
FLCA
ISV-
-GIIL
SVFI
WLF
TLAT
AILF
LI--
--HLT
IVIG
FFLV
FFVV
VVCN
FL-
QIT
WLL
MSL
NCI
LDPV
VYFF
A---
---Q
MLF
SVVG
VFVV
CFLP
HHAC
Q--
Zebrafi
sh--P
IFYG
VVFV
LGLI
ANCY
ALYV
--IY
MTN
LTVA
DLLF
VSAL
PFW
I-----
GTFF
FIN
TYGS
ILFL
TVIS
INRG
AIIS
VLIW
AFIL
SASI
PQL-
--IT
HFSI
IGLF
FLVF
LMVI
ICN
-----L
RMLC
AVIG
VFVI
CFLP
HHVV
---Q
ITLM
LMGI
NCL
LDPL
VYCF
A---
18Supplementary data Search for PAF receptor in T. cruzi • Daniel Fábio Kawano et al.
Res
cale
d co
nser
vatio
n sc
ores
of t
he a
min
o ac
ids f
rom
tran
smem
bran
e do
mai
ns fr
om T
rypa
noso
ma
cruz
i hyp
othe
tical
pro
tein
s: D
omai
n I
Tc00
. 10
47
0535
08
21
5.6
Tc00
. 10
47
0535
08
72
3.24
Tc00
. 10
47
0535
09
79
1.60
Tc00
. 10
47
0535
06
75
3.30
Tc00
. 10
47
0535
05
19
3.74
Tc00
. 10
47
0535
03
46
3.20
Tc00
. 10
47
0535
04
13
7.150
Tc00
. 10
47
0535
06
46
7.4
Tc00
. 10
47
0535
09
79
9.100
Tc00
. 10
47
0535
08
64
1.20
0
Tc00
. 10
47
0535
11
78
7.30
Tc00
. 10
47
0535
10
30
5.79
Tc00
. 10
47
0535
10
86
1.94
Tc00
. 10
47
0535
11
92
3.71
Tc00
. 10
47
0535
11
92
1.71
Tc00
. 10
47
0535
03
73
5.30
Tc00
. 10
47
0535
10
35
5.19
0
Tc00
. 10
47
0535
09
71
9.56
Tc00
. 10
47
0535
08
31
7.70
Tc00
. 10
47
0535
09
84
9.30
Tc00
. 10
47
0535
10
72
9.29
0
Tc00
. 10
47
0535
04
13
7.140
Tc00
. 10
47
0535
09
79
9.90
Tc00
. 10
47
0535
07
96
9.30
Tc00
. 10
47
0535
11
00
1.50
Tc00
. 10
47
0535
07
68
1.50
Tc00
. 10
47
0535
06
82
5.24
Tc00
. 10
47
0535
11
50
7.60
Tc00
. 10
47
0535
08
53
5.30
-95
9543
544
-5
--
11-
911
191
4345
--
-31
32-
021
996
3299
00
3131
8961
3161
-46
9422
2020
2231
92-
61-
4449
-90
2936
5156
020
2048
9827
4098
40-
3530
3976
7639
4818
1599
-76
7623
2341
7636
4796
1212
9653
7198
5398
9926
4698
--
9896
2972
9746
1315
721
330
2553
3723
2368
678
667
699
025
13-
-13
6822
6328
1925
2821
715
184
176
2727
55
381
51
3922
2928
2727
285
985
512
1012
2537
304
990
6764
6497
3998
3739
3736
9965
098
980
9733
338
036
3841
9838
-20
6519
5858
0-
9914
-14
334
5934
9999
340
015
3598
2022
3831
35-
799
9911
1137
9965
9999
9912
2446
3330
3033
3744
9948
990
39
4329
-99
2541
3232
9830
1649
3049
4243
2649
4141
4998
3129
5073
9898
016
23-
4599
5299
996
4999
5249
520
4424
9821
2198
648
4698
9999
9899
698
-46
2399
4343
3799
2927
9927
3837
449
2929
937
41
1738
4447
9916
23-
019
4964
6464
4844
9748
9720
6365
643
364
6444
4464
4668
6946
9968
160
6447
6464
8181
2581
8181
8262
6599
6464
9981
632
6565
8182
024
1362
6237
3916
1638
831
208
2098
84
2038
3820
388
3221
3734
360
3098
99-
1799
--
8077
8015
7715
6765
3233
5959
3380
2274
2132
1222
780
4049
-10
81-
-94
5097
050
082
4250
6439
3964
9467
9942
6547
4849
6737
67-
4032
6565
1961
2466
6166
66-
9914
2323
1419
90
2966
1317
2316
9963
6366
9823
2317
57-
9857
9840
-3
4098
9840
1738
5242
540
420
9998
9919
3956
4545
9911
-47
1147
52-
9947
2323
4799
300
9999
9999
509
4999
4219
1393
9352
99-
1099
1093
-0
323
332
5241
949
98-
9682
155
927
31
19Supplementary data Search for PAF receptor in T. cruzi • Daniel Fábio Kawano et al.
Res
cale
d co
nser
vatio
n sc
ores
of t
he a
min
o ac
ids f
rom
tran
smem
bran
e do
mai
ns fr
om T
rypa
noso
ma
cruz
i hyp
othe
tical
pro
tein
s: D
omai
n II
Tc00
. 10
47
0535
08
21
5.6
Tc00
. 10
47
0535
08
72
3.24
Tc00
. 10
47
0535
09
79
1.60
Tc00
. 10
47
0535
06
75
3.30
Tc00
. 10
47
0535
05
19
3.74
Tc00
. 10
47
0535
03
46
3.20
Tc00
. 10
47
0535
04
13
7.150
Tc00
. 10
47
0535
06
46
7.4
Tc00
. 10
47
0535
09
79
9.100
Tc00
. 10
47
0535
08
64
1.20
0
Tc00
. 10
47
0535
11
78
7.30
Tc00
. 10
47
0535
10
30
5.79
Tc00
. 10
47
0535
10
86
1.94
Tc00
. 10
47
0535
11
92
3.71
Tc00
. 10
47
0535
11
92
1.71
Tc00
. 10
47
0535
03
73
5.30
Tc00
. 10
47
0535
10
35
5.19
0
Tc00
. 10
47
0535
09
71
9.56
Tc00
. 10
47
0535
08
31
7.70
Tc00
. 10
47
0535
09
84
9.30
Tc00
. 10
47
0535
10
72
9.29
0
Tc00
. 10
47
0535
04
13
7.140
Tc00
. 10
47
0535
09
79
9.90
Tc00
. 10
47
0535
07
96
9.30
Tc00
. 10
47
0535
11
00
1.50
Tc00
. 10
47
0535
07
68
1.50
Tc00
. 10
47
0535
06
82
5.24
Tc00
. 10
47
0535
11
50
7.60
Tc00
. 10
47
0535
08
53
5.30
-97
097
--
9743
2743
-98
9598
3330
-33
-30
-19
8199
-19
1943
4-
4047
40-
-27
2220
2245
128
2499
07
99-
07
4847
26-
4747
120
-99
099
10-
936
2336
5354
5453
714
971
524
949
2627
-27
271
5331
9921
9928
-30
260
2611
2017
1339
3324
4130
3324
290
36-
1414
912
042
4142
99-
4317
3817
4048
9999
1240
1714
1440
177
9942
-99
9943
9919
2236
2223
2241
351
3512
2118
1399
1434
980
1434
2424
40-
2323
922
990
00
2760
9826
9326
2627
3027
6326
6264
5926
6293
61-
-93
9315
6022
249
2426
9898
313
3122
2312
712
9926
140
9926
2516
--
2525
2531
54
174
3157
08
268
2216
3430
238
3424
998
3439
11-
9925
2426
3631
1199
1138
9940
9933
996
996
098
5398
982
5398
3899
991
9999
9936
1225
4525
2599
9950
4150
2299
83
4850
4750
450
476
9999
099
9999
1615
660
6646
1122
3164
3131
2536
3299
3149
9963
3149
2726
4740
3333
48-
6397
097
2239
9650
250
2197
9597
9650
9595
3650
9597
2197
9921
2197
-63
6799
6762
4845
6363
6342
4562
6223
1849
2416
1849
4650
490
4949
49-
174
254
47
976
06
992
62
86
2710
196
2721
9821
214
421
-33
435
411
2-
145
148
1051
4898
10
984
10
3812
3630
1212
3799
9930
1530
3099
-28
9928
2899
3329
2828
1929
028
1931
159
9931
3110
9911
6642
6699
14-
41-
416
-50
485
4144
6-
4144
30
426
4242
4322
14-
99-
134
9924
-24
3-
105
1231
3614
-31
3640
07
107
79
9937
-2
-58
9912
--
-23
-98
--
097
--
097
41-
4155
--
4116
21-
0-
9067
23-
--
16-
--
--
33-
--
3326
-0
99-
-1
67
20Supplementary data Search for PAF receptor in T. cruzi • Daniel Fábio Kawano et al.
Res
cale
d co
nser
vatio
n sc
ores
of t
he a
min
o ac
ids f
rom
tran
smem
bran
e do
mai
ns fr
om T
rypa
noso
ma
cruz
i hyp
othe
tical
pro
tein
s: D
omai
n II
I
Tc00
. 10
47
0535
08
21
5.6
Tc00
. 10
47
0535
08
72
3.24
Tc00
. 10
47
0535
09
79
1.60
Tc00
. 10
47
0535
06
75
3.30
Tc00
. 10
47
0535
05
19
3.74
Tc00
. 10
47
0535
03
46
3.20
Tc00
. 10
47
0535
04
13
7.150
Tc00
. 10
47
0535
06
46
7.4
Tc00
. 10
47
0535
09
79
9.100
Tc00
. 10
47
0535
08
64
1.20
0
Tc00
. 10
47
0535
11
78
7.30
Tc00
. 10
47
0535
10
30
5.79
Tc00
. 10
47
0535
10
86
1.94
Tc00
. 10
47
0535
11
92
3.71
Tc00
. 10
47
0535
11
92
1.71
Tc00
. 10
47
0535
03
73
5.30
Tc00
. 10
47
0535
10
35
5.19
0
Tc00
. 10
47
0535
09
71
9.56
Tc00
. 10
47
0535
08
31
7.70
Tc00
. 10
47
0535
09
84
9.30
Tc00
. 10
47
0535
10
72
9.29
0
Tc00
. 10
47
0535
04
13
7.140
Tc00
. 10
47
0535
09
79
9.90
Tc00
. 10
47
0535
07
96
9.30
Tc00
. 10
47
0535
11
00
1.50
Tc00
. 10
47
0535
07
68
1.50
Tc00
. 10
47
0535
06
82
5.24
Tc00
. 10
47
0535
11
50
7.60
Tc00
. 10
47
0535
08
53
5.30
--
--
--
65-
65-
84-
--
--
--
9984
-67
6750
-96
310
--
--
--
-40
-40
-99
--
-94
--
-41
0-
1111
78-
2415
63-
-34
34-
99-
3399
3316
33-
--
44-
--
440
-33
3387
-95
1617
--
9999
-0
-8
08
3499
-9
1299
--
9941
3512
88
9930
1331
99-
9929
29-
2999
3629
361
0-
3519
1634
-5
1769
1369
6920
096
9-
-99
8585
-98
9985
9885
8547
-47
497
47-
9829
498
5050
5447
085
--
240
4032
492
5049
5099
45-
1448
2212
4699
5317
6750
5029
4518
046
-99
1919
1499
9934
9934
1999
6330
4545
2843
09
4699
3535
9964
9929
43-
4160
6010
141
591
5924
2055
5699
9956
342
980
3059
5950
1942
563
-96
5555
8984
9685
8485
991
3972
5555
6726
8199
3998
8686
730
1926
26-
1422
2299
2514
3325
3323
39
1315
711
2824
4439
034
3435
324
1228
-6
9999
2565
665
6565
2262
04
287
311
2399
863
6565
3362
2311
118
2939
3923
029
160
1618
-19
1428
9713
5097
536
2416
1656
9925
3550
1820
2626
9937
2037
3737
35-
9934
2134
3216
2241
3620
3737
2917
2232
160
4130
3099
6741
2767
2743
-38
980
4299
2342
9798
4318
1849
443
2423
1532
1818
1161
3214
6114
9711
4098
32-
9960
4362
6061
6161
890
1330
6059
9999
9999
1899
5918
5932
998
5657
-55
550
9933
9959
5999
108
355
2144
9999
4220
4446
2046
2549
4143
9944
420
5299
9910
4747
9942
1914
04
4729
2934
4847
4648
4631
3699
146
370
4737
4746
5495
9538
845
947
3157
9696
5497
5723
9723
40-
9932
9732
3156
2320
1455
2323
920
797
5634
3519
1933
3735
2237
2252
-31
2519
3524
5099
205
518
8-
4952
050
43
21Supplementary data Search for PAF receptor in T. cruzi • Daniel Fábio Kawano et al.
Res
cale
d co
nser
vatio
n sc
ores
of t
he a
min
o ac
ids f
rom
tran
smem
bran
e do
mai
ns fr
om T
rypa
noso
ma
cruz
i hyp
othe
tical
pro
tein
s: D
omai
n IV
Tc00
. 10
47
0535
08
21
5.6
Tc00
. 10
47
0535
08
72
3.24
Tc00
. 10
47
0535
09
79
1.60
Tc00
. 10
47
0535
06
75
3.30
Tc00
. 10
47
0535
05
19
3.74
Tc00
. 10
47
0535
03
46
3.20
Tc00
. 10
47
0535
04
13
7.150
Tc00
. 10
47
0535
06
46
7.4
Tc00
. 10
47
0535
09
79
9.100
Tc00
. 10
47
0535
08
64
1.20
0
Tc00
. 10
47
0535
11
78
7.30
Tc00
. 10
47
0535
10
30
5.79
Tc00
. 10
47
0535
10
86
1.94
Tc00
. 10
47
0535
11
92
3.71
Tc00
. 10
47
0535
11
92
1.71
Tc00
. 10
47
0535
03
73
5.30
Tc00
. 10
47
0535
10
35
5.19
0
Tc00
. 10
47
0535
09
71
9.56
Tc00
. 10
47
0535
08
31
7.70
Tc00
. 10
47
0535
09
84
9.30
Tc00
. 10
47
0535
10
72
9.29
0
Tc00
. 10
47
0535
04
13
7.140
Tc00
. 10
47
0535
09
79
9.90
Tc00
. 10
47
0535
07
96
9.30
Tc00
. 10
47
0535
11
00
1.50
Tc00
. 10
47
0535
07
68
1.50
Tc00
. 10
47
0535
06
82
5.24
Tc00
. 10
47
0535
11
50
7.60
Tc00
. 10
47
0535
08
53
5.30
-40
4050
--
45-
4599
2937
9737
5397
5047
380
2247
4761
-36
5412
-86
7777
57-
8679
-79
9994
9486
8647
8657
8494
099
7676
39-
9655
9986
9740
4025
-97
96-
9699
323
973
097
2599
5939
052
529
-99
2250
9729
9999
30-
2999
-99
2226
1028
2623
2830
526
025
2224
128
82
9999
4497
9773
-44
58-
580
9898
9773
1697
7341
9898
735
698
417
9914
4319
1818
8199
1981
9981
4358
432
440
3281
5851
8283
4343
6043
160
8333
9999
9950
3399
2232
2256
024
358
563
5036
587
462
526
385
161
2621
66
3099
2132
9932
2125
1921
422
2130
996
244
02
2723
165
2620
7036
3629
2070
3020
3023
5699
5771
057
2970
5724
5623
2427
7036
5755
9875
00
3471
7524
7124
1971
7171
4558
7134
1999
7173
1919
7373
7158
6599
1258
5898
5012
5950
5926
3053
2321
5223
980
5399
490
256
5852
58-
528
9999
5921
2838
2038
1799
698
570
9859
-2
5734
5656
357
1957
-21
32-
-37
4932
8449
8499
8284
4433
9944
37-
099
8231
3254
4199
6-
119
--
628
1938
2838
9933
420
590
206
-19
1662
1617
2058
9835
-35
38-
-97
3038
2714
2798
898
230
2723
97-
36-
3799
9897
9820
98-
3995
--
9529
9517
2917
3314
3296
2099
9695
--
--
3030
3330
033
-52
796
9617
167
2215
22-
03
9618
6196
1798
5553
-0
119
2014
6399
2244
7373
1739
4496
3996
-73
1432
9917
3217
7344
9798
1011
3997
2416
260
4398
9813
1743
6017
60-
498
-98
0-
1399
4598
4199
9911
5756
955
1155
2727
5724
558
248
-15
--
-21
-57
095
8413
-22
8555
--
9994
5621
21-
2556
-25
--
16-
--
--
-62
-19
17-
--
52-
-0
99
22Supplementary data Search for PAF receptor in T. cruzi • Daniel Fábio Kawano et al.
Res
cale
d co
nser
vatio
n sc
ores
of t
he a
min
o ac
ids f
rom
tran
smem
bran
e do
mai
ns fr
om T
rypa
noso
ma
cruz
i hyp
othe
tical
pro
tein
s: D
omai
n V
Tc00
. 10
47
0535
08
21
5.6
Tc00
. 10
47
0535
08
72
3.24
Tc00
. 10
47
0535
09
79
1.60
Tc00
. 10
47
0535
06
75
3.30
Tc00
. 10
47
0535
05
19
3.74
Tc00
. 10
47
0535
03
46
3.20
Tc00
. 10
47
0535
04
13
7.150
Tc00
. 10
47
0535
06
46
7.4
Tc00
. 10
47
0535
09
79
9.100
Tc00
. 10
47
0535
08
64
1.20
0
Tc00
. 10
47
0535
11
78
7.30
Tc00
. 10
47
0535
10
30
5.79
Tc00
. 10
47
0535
10
86
1.94
Tc00
. 10
47
0535
11
92
3.71
Tc00
. 10
47
0535
11
92
1.71
Tc00
. 10
47
0535
03
73
5.30
Tc00
. 10
47
0535
10
35
5.19
0
Tc00
. 10
47
0535
09
71
9.56
Tc00
. 10
47
0535
08
31
7.70
Tc00
. 10
47
0535
09
84
9.30
Tc00
. 10
47
0535
10
72
9.29
0
Tc00
. 10
47
0535
04
13
7.140
Tc00
. 10
47
0535
09
79
9.90
Tc00
. 10
47
0535
07
96
9.30
Tc00
. 10
47
0535
11
00
1.50
Tc00
. 10
47
0535
07
68
1.50
Tc00
. 10
47
0535
06
82
5.24
Tc00
. 10
47
0535
11
50
7.60
Tc00
. 10
47
0535
08
53
5.30
23-
--
6199
1861
1835
34-
3635
1436
61-
--
1634
9918
--
-0
1450
--
-50
078
5078
7455
-56
6365
5699
--
-58
7654
5376
--
5678
66-
-37
160
7616
7621
9976
6767
5067
6728
--
1675
4514
6566
6767
229
--
1524
9915
2415
1017
3710
2121
1037
8-
-11
924
109
70
3713
9862
6263
9899
5698
5664
3310
554
1755
5959
--
3230
5829
014
2698
5537
66
9631
3796
3196
-18
3414
9694
1435
34-
-98
9931
330
3067
3536
9961
6134
6192
5461
5494
5413
5861
5458
4756
6161
3445
5454
5456
5658
023
2323
023
2092
2392
2313
2330
4189
3028
899
997
418
3020
282
4130
6799
9999
3468
9934
9939
3150
2539
4125
2567
44
210
3445
4320
36-
3813
9999
350
1726
026
5052
2336
3128
3624
2399
9946
4534
9967
3223
-99
4599
9998
3545
7135
7163
3799
4698
9746
6282
9999
1981
20
-9
82-
824
5656
3936
798
3698
1030
9823
1720
2323
9924
2436
936
0-
5538
-40
468
6851
488
4148
4141
9920
928
439
5119
00
4821
3527
-47
1399
9911
2222
1140
1142
4042
4295
9862
6243
6297
9861
6140
9820
39-
099
4742
7810
1021
8778
2287
2233
987
3221
9632
011
9999
34
3469
-28
8787
3273
9898
9817
7350
1750
9797
3949
2217
4921
4851
510
1273
1272
4699
746
5817
1726
1057
9510
9520
230
6054
2860
539
5757
5251
1622
9952
972
145
7878
6678
868
7868
7768
9597
-94
9797
6699
9963
9963
310
9966
5278
1416
164
018
-0
-7
983
9898
9898
1837
9898
15-
637
995
3718
270
2626
2725
--
25-
9926
413
738
13-
2620
2031
-0
2234
2210
9920
-14
1428
--
--
-94
4948
4995
4849
-96
55
54-
--
4899
047
17
23Supplementary data Search for PAF receptor in T. cruzi • Daniel Fábio Kawano et al.
Res
cale
d co
nser
vatio
n sc
ores
of t
he a
min
o ac
ids f
rom
tran
smem
bran
e do
mai
ns fr
om T
rypa
noso
ma
cruz
i hyp
othe
tical
pro
tein
s: D
omai
n V
I
Tc00
. 10
47
0535
08
21
5.6
Tc00
. 10
47
0535
08
72
3.24
Tc00
. 10
47
0535
09
79
1.60
Tc00
. 10
47
0535
06
75
3.30
Tc00
. 10
47
0535
05
19
3.74
Tc00
. 10
47
0535
03
46
3.20
Tc00
. 10
47
0535
04
13
7.150
Tc00
. 10
47
0535
06
46
7.4
Tc00
. 10
47
0535
09
79
9.100
Tc00
. 10
47
0535
08
64
1.20
0
Tc00
. 10
47
0535
11
78
7.30
Tc00
. 10
47
0535
10
30
5.79
Tc00
. 10
47
0535
10
86
1.94
Tc00
. 10
47
0535
11
92
3.71
Tc00
. 10
47
0535
11
92
1.71
Tc00
. 10
47
0535
03
73
5.30
Tc00
. 10
47
0535
10
35
5.19
0
Tc00
. 10
47
0535
09
71
9.56
Tc00
. 10
47
0535
08
31
7.70
Tc00
. 10
47
0535
09
84
9.30
Tc00
. 10
47
0535
10
72
9.29
0
Tc00
. 10
47
0535
04
13
7.140
Tc00
. 10
47
0535
09
79
9.90
Tc00
. 10
47
0535
07
96
9.30
Tc00
. 10
47
0535
11
00
1.50
Tc00
. 10
47
0535
07
68
1.50
Tc00
. 10
47
0535
06
82
5.24
Tc00
. 10
47
0535
11
50
7.60
Tc00
. 10
47
0535
08
53
5.30
--
--
21-
2721
2820
1328
1322
9913
-44
--
-8
8-
-20
-0
16-
--
-74
-96
7499
2345
9993
8245
93-
54-
--
33
--
91-
06
--
--
46-
9046
9215
8821
393
9539
-97
4040
-99
99-
-88
-91
0-
--
-14
-14
1414
140
011
9994
11-
125
5-
55
16-
153
903
--
--
40-
4840
4849
4212
4844
2148
-46
9999
-21
2124
1740
3947
015
2626
079
1554
7954
7995
7996
780
961
7829
2999
7878
1779
64
968
040
4012
110
1611
1640
2929
1099
9410
1312
9191
4039
3923
4289
9639
2753
2525
350
5399
079
4240
3926
2121
2636
3840
4024
33
5438
3921
2645
5020
2099
2550
4025
3916
2855
422
184
9911
99
110
021
2212
2839
2079
9999
7997
7920
9719
9533
7946
029
4679
9997
9730
6464
9912
2265
6679
4986
8686
8649
8686
8698
9842
4286
3842
86-
2424
4886
860
1899
4839
2736
2929
5056
3699
5699
4599
4142
3220
4299
-55
5599
9999
099
3522
1837
9999
9910
099
990
9999
4846
9938
2799
11-
3333
4240
4064
9921
9919
9910
4141
898
1098
9898
306
378
190
89
-43
4362
3939
9925
4441
631
3433
330
9934
1599
1561
7699
7-
577
1-
3535
768
811
3460
5859
7615
22
543
1523
4322
-25
2323
-0
236
997
70
9999
9999
2417
618
9942
4261
9999
9999
9999
1533
33-
1933
6212
4646
42-
-43
1514
4231
02
9999
280
211
8010
9563
8080
--
803
9863
6317
--
8034
804
630
027
276
160
-16
-36
279
99-
-99
71
3131
2-
-11
2018
277
1951
1515
34-
51-
--
2330
9727
--
2736
049
4991
--
594
992
-16
055
5529
-0
--
-34
1916
10-
-10
3024
2222
16-
-11
2631
2-
99
24Supplementary data Search for PAF receptor in T. cruzi • Daniel Fábio Kawano et al.
Res
cale
d co
nser
vatio
n sc
ores
of t
he a
min
o ac
ids f
rom
tran
smem
bran
e do
mai
ns fr
om T
rypa
noso
ma
cruz
i hyp
othe
tical
pro
tein
s: D
omai
n V
II
Tc00
. 10
47
0535
08
21
5.6
Tc00
. 10
47
0535
08
72
3.24
Tc00
. 10
47
0535
09
79
1.60
Tc00
. 10
47
0535
06
75
3.30
Tc00
. 10
47
0535
05
19
3.74
Tc00
. 10
47
0535
03
46
3.20
Tc00
. 10
47
0535
04
13
7.150
Tc00
. 10
47
0535
06
46
7.4
Tc00
. 10
47
0535
09
79
9.100
Tc00
. 10
47
0535
08
64
1.20
0
Tc00
. 10
47
0535
11
78
7.30
Tc00
. 10
47
0535
10
30
5.79
Tc00
. 10
47
0535
10
86
1.94
Tc00
. 10
47
0535
11
92
3.71
Tc00
. 10
47
0535
11
92
1.71
Tc00
. 10
47
0535
03
73
5.30
Tc00
. 10
47
0535
10
35
5.19
0
Tc00
. 10
47
0535
09
71
9.56
Tc00
. 10
47
0535
08
31
7.70
Tc00
. 10
47
0535
09
84
9.30
Tc00
. 10
47
0535
10
72
9.29
0
Tc00
. 10
47
0535
04
13
7.140
Tc00
. 10
47
0535
09
79
9.90
Tc00
. 10
47
0535
07
96
9.30
Tc00
. 10
47
0535
11
00
1.50
Tc00
. 10
47
0535
07
68
1.50
Tc00
. 10
47
0535
06
82
5.24
Tc00
. 10
47
0535
11
50
7.60
Tc00
. 10
47
0535
08
53
5.30
4399
9940
4643
845
861
9793
9398
9893
4058
4242
4592
1322
980
1131
5737
1414
4246
3726
4526
2399
9937
4848
3741
3637
3728
3330
2238
029
4242
1221
2197
9812
4147
4199
4545
2543
4325
9744
3838
045
4322
9919
2443
2220
1414
194
2026
9626
919
3023
2828
230
1815
1516
3027
208
1299
2025
4499
9999
1044
408
4043
4545
9999
9999
9944
3737
9946
440
9940
428
2940
9898
9543
4098
4298
3596
413
2525
295
599
9934
9596
2596
7838
097
507
719
-50
30-
3031
5128
3349
4933
1034
55
9917
1310
2710
09
3299
3636
47
9925
525
4429
284
2626
43
2734
340
2928
2545
3513
2637
540
042
8854
-83
-7
9977
3770
7035
3892
9292
4582
726
7759
8831
483
9999
2010
3-
8-
820
1919
9999
1819
326
67
9999
3819
415
310
5130
3054
2251
-21
-0
5151
7396
9654
5373
9999
2794
9596
7297
5328
7299
4545
9999
99-
99-
4042
9999
9999
9999
990
038
--
299
1346
9939
--
-13
99-
-99
-27
43-
4-
-4
128
1919
26-
-20
1130
-14
0-
--
4739
-36
3736
3223
-22
-0
2234
9925
2538
3734
2642
--
1332
42-
-28
9842
3899
3885
9999
61-
060
2817
--
3944
4185
85-
5920
5999
--
3675
9973
7573
5199
3838
-0
3835
51-
-50
3937
5052
-50
7420
47-
-99
2747
5725
576
2367
67-
067
9999
--
1118
1419
3564
566
1228
4242
9631
2841
2941
4021
2425
-0
2496
23-
-17
9798
22-
9998
2222
3199
9969
1131
998
9929
-32
3299
9931
68-
--
034
3099
-99
6430
6498
2323
4895
980
960
--
4327
9898
2747
--
-5
1815
33-
459
994
1711
11-
1717
9918
99-
--
--
--
--
--
-19
17-
-8
0-
-