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SD BIOLINE Lymphatic Filariasis IgG4 Rapid Test Procedure 1

SD BIOLINE Lymphatic Filariasis IgG4 Rapid Test Procedure · 2018-09-11 · Lymphatic Filariasis IgG4 rapid test pouch. Look at the desiccant. If the beads are white and yellow, the

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Page 1: SD BIOLINE Lymphatic Filariasis IgG4 Rapid Test Procedure · 2018-09-11 · Lymphatic Filariasis IgG4 rapid test pouch. Look at the desiccant. If the beads are white and yellow, the

SD BIOLINE Lymphatic Filariasis IgG4 Rapid Test Procedure

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Page 2: SD BIOLINE Lymphatic Filariasis IgG4 Rapid Test Procedure · 2018-09-11 · Lymphatic Filariasis IgG4 rapid test pouch. Look at the desiccant. If the beads are white and yellow, the

• Test device with desiccant in individual foil pouch (25)

• Assay diluent (one vial)

• Disposable capillary pipettes (25)

• Lancet (25)

• Alcohol swab (25)

• Instructions (one)

List of items included in the kit

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Page 3: SD BIOLINE Lymphatic Filariasis IgG4 Rapid Test Procedure · 2018-09-11 · Lymphatic Filariasis IgG4 rapid test pouch. Look at the desiccant. If the beads are white and yellow, the

Disposable gloves

Gauze

Bandage

Sharps container

Permanent pen

Timer

Additional supplies needed

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Page 4: SD BIOLINE Lymphatic Filariasis IgG4 Rapid Test Procedure · 2018-09-11 · Lymphatic Filariasis IgG4 rapid test pouch. Look at the desiccant. If the beads are white and yellow, the

Put on the gloves. Use new gloves for each person.

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Page 5: SD BIOLINE Lymphatic Filariasis IgG4 Rapid Test Procedure · 2018-09-11 · Lymphatic Filariasis IgG4 rapid test pouch. Look at the desiccant. If the beads are white and yellow, the

Check the expiration date on the test.

Do not use this test if the expiration date has passed. Dispose of this test and get a new one.

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Page 6: SD BIOLINE Lymphatic Filariasis IgG4 Rapid Test Procedure · 2018-09-11 · Lymphatic Filariasis IgG4 rapid test pouch. Look at the desiccant. If the beads are white and yellow, the

Open the SD BIOLINE Lymphatic Filariasis IgG4 rapid test pouch.

Look at the desiccant. If the beads are white and yellow, the test is fine to use. If some of the beads are green, use another test and dispose of the test with green beads.

Throw away the desiccant.

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Page 7: SD BIOLINE Lymphatic Filariasis IgG4 Rapid Test Procedure · 2018-09-11 · Lymphatic Filariasis IgG4 rapid test pouch. Look at the desiccant. If the beads are white and yellow, the

SD BIOLINE Lymphatic Filariasis IgG4 Rapid Test

Assay diluent well

Sample well

Air openings

Result window

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Page 8: SD BIOLINE Lymphatic Filariasis IgG4 Rapid Test Procedure · 2018-09-11 · Lymphatic Filariasis IgG4 rapid test pouch. Look at the desiccant. If the beads are white and yellow, the

Write the participant identification number on the test using a permanent pen.

If you use labels, do not obstruct the air openings.

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Page 9: SD BIOLINE Lymphatic Filariasis IgG4 Rapid Test Procedure · 2018-09-11 · Lymphatic Filariasis IgG4 rapid test pouch. Look at the desiccant. If the beads are white and yellow, the

Open the alcohol swab. Clean the finger that will be pricked with the alcohol swab using a circular motion for 10 seconds. Allow to air-dry.

The best finger to prick is the fourth finger on the hand that is used the least often.

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Page 10: SD BIOLINE Lymphatic Filariasis IgG4 Rapid Test Procedure · 2018-09-11 · Lymphatic Filariasis IgG4 rapid test pouch. Look at the desiccant. If the beads are white and yellow, the

Using the lancet, prick the participant’s finger to get a drop of blood.

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Page 11: SD BIOLINE Lymphatic Filariasis IgG4 Rapid Test Procedure · 2018-09-11 · Lymphatic Filariasis IgG4 rapid test pouch. Look at the desiccant. If the beads are white and yellow, the

Discard the lancet into the sharps container.

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Page 12: SD BIOLINE Lymphatic Filariasis IgG4 Rapid Test Procedure · 2018-09-11 · Lymphatic Filariasis IgG4 rapid test pouch. Look at the desiccant. If the beads are white and yellow, the

Wait until a drop of blood has collected on the finger.

Using the capillary pipette, collect blood up to the fill line(10 µl).

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Page 13: SD BIOLINE Lymphatic Filariasis IgG4 Rapid Test Procedure · 2018-09-11 · Lymphatic Filariasis IgG4 rapid test pouch. Look at the desiccant. If the beads are white and yellow, the

Add the blood into the round sample well.

Let the blood absorb into the pad.

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Page 14: SD BIOLINE Lymphatic Filariasis IgG4 Rapid Test Procedure · 2018-09-11 · Lymphatic Filariasis IgG4 rapid test pouch. Look at the desiccant. If the beads are white and yellow, the

Discard the capillary pipette into the sharps container.

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Page 15: SD BIOLINE Lymphatic Filariasis IgG4 Rapid Test Procedure · 2018-09-11 · Lymphatic Filariasis IgG4 rapid test pouch. Look at the desiccant. If the beads are white and yellow, the

Add four drops of assay diluent into the square assay diluent well.

4 drops

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Page 16: SD BIOLINE Lymphatic Filariasis IgG4 Rapid Test Procedure · 2018-09-11 · Lymphatic Filariasis IgG4 rapid test pouch. Look at the desiccant. If the beads are white and yellow, the

Wait 30 minutes

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Page 17: SD BIOLINE Lymphatic Filariasis IgG4 Rapid Test Procedure · 2018-09-11 · Lymphatic Filariasis IgG4 rapid test pouch. Look at the desiccant. If the beads are white and yellow, the

Read test results.

‘C’ is the control line.

‘L’ is the test line for antibodies toWb123 (lymphatic filariasis).

Positive (reactive)

Invalid results

C L

Negative (nonreactive)

C L

C L

C L C L

Results window

Valid results

If the test is invalid, it is recommended that the specimen be re-tested.

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Page 18: SD BIOLINE Lymphatic Filariasis IgG4 Rapid Test Procedure · 2018-09-11 · Lymphatic Filariasis IgG4 rapid test pouch. Look at the desiccant. If the beads are white and yellow, the

Record the results on the result form.

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Page 19: SD BIOLINE Lymphatic Filariasis IgG4 Rapid Test Procedure · 2018-09-11 · Lymphatic Filariasis IgG4 rapid test pouch. Look at the desiccant. If the beads are white and yellow, the

For more information or training-related questions, contact PATH’s Diagnostics Program at [email protected]

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