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GIORGI KHAREBAVA, PHDAddress: University of Maryland Shock Trauma and Anesthesiology Research Center655 West Baltimore Street, BRB Room #6-017, Baltimore, Maryland 21201Phone: (502) 641-5617Email: kharebava@gmail.com

SCIENTIST | SENIOR SCIENTISTFocus: Pharmaceutical / Biotech

QUALIFICATIONS PROFILE

Experienced research scientistist / biochemist, offering advanced expertise in molecular biology, molecular neuroscience, cell signaling, and gene expression complemented with progressive experience in cell death and survival mechanisms andgrowth factor signaling. Proficient in executing a wide range of laboratory methods and completing experiment in extremely diverse research settings, includingmolecular neuroscience, cancer research, basic cell signaling, flavonoids, andmanufacturing technology development. Exemplify strong data analysis, interpretation and documentation skills to produce comprehensive outcomes. Committed to ongoing professional development to advance knowledge and increase technical competencies.

TECHNICAL ACUMEN

Cellular Biology- Primary Cortical / Hippocampal Neuron / Glial / Astrocyte Cell Cultures, In Vitro neuronal Death Models, Transfections, Electroporation, Immunocytostaining, Fluorescent Microscopy, Metabolic Labeling

Biochemistry- Protein, Peptide / Natural Compound Extraction / Purification, Chromatography,HPLC, 2D Gel Electrophoresis, Western Blotting, Immunoprecipitations, Protein Kinase / Other Enzymatic, Radioactive / Cell-Based Assays

Molecular Biology- DNA / RNA Purification, Cloning, Mutagenesis, Quantitative PCR, RNA Interference, Promoter Analysis, Methyl-Specific PCR / Bisulfate Sequencing, Chromatin Immunoprecipitation (Chip)

POSTDOCTORAL RESEARCH EXPERIENCE

Nov 2009-Present ~ Cell Cycle Mechanisms In Neuronal Death and Brain InjuryShock Trauma and Anaesthesiology Research CenterUniversity of Maryland ~ Baltimore, MDProvide oversight to all aspects of in vitro experiments addressing scientific problems in gene expression, cell signaling, other matters related to neuronal d

eath / survival, microglial activation after injury, and neuronal-glial interactions. Facilitate trainings for junior researchers.- Developed key data for funding renewal- Thrived in determining new mechanisms related to cell cycle, which are also involved in neuronal death

Nov 2008-Nov 2009 ~ Epigenetic Regulation in CancerDepartment of MedicineUniversity of Louisville ~ Louisville, KYAdministered the entire set of in vitro experiments concerning scientific proble

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ms in epigenetic regulation of cancer-related gene expression.- Determined methylation patterns in several gene promoter areas in apoptotic cells; provided documentation of original experimental results to several ongoingprojects in the laboratory

Jan 2004-Nov 2008 ~ Neurotrophin Induced Survival Signaling in Cortical NeuronsLaboratory of Neurosignaling, Kentucky Spinal Cord Injury Research CenterUniversity of Louisville ~ Louisville, KYOrganized and implemented in vitro experiments in coordination with principal investigator. Interacted with collaborators and represented laboratory on nationallevel at conferences and meetings, and contributed data to several ongoing projects and outgoing publication writing.- Assigned to represent the laboratory on national and international meetings- Wrote and and co-authored five research papers presenting original findings- Constantly developed key data for funding renewal and reporting- Introduced and established RNA interference, Methyl Specific PCR and other advanced techniques in the laboratory- Trained and supervised junior researchers

Nov 2002-Nov 2003 ~ MAPK Signaling and ApoptosisGene expression laboratories, Faculty of Life SciencesUniversity of Manchester ~ Manchester, United Kingdom- Structured and performed in vitro experiments designed together with principalinvestigator. Created new kinase substrate screening techniques and determined

several candidate targets of the JNK activity in cells.

EARLIER EXPERIENCE

Oct 2000-June 2002 ~ ScientistITA Limited (Nutraceuticals) ~ Tbilisi, Georgia

May 1997-Nov 2002 ~ Executive DirectorYoung Scientists Development Center ~ Tbilisi, Georgia

June 1996-Nov 2002 ~ Research AssistantInstitute of Medical Biotechnology, Georgian Academy of Sciences ~ Tbilisi, Geor

gia

June 1996-July 1998 ~ ScientistLB Limited (Pharmaceutical company) ~ Tbilisi, Georgia

EDUCATION

2001 ~ Doctor of Philosophy in Biochemistry, Georgian Academy of Sciences ~ Tbilisi, Georgia1995 ~ University Diploma in Biology and Biochemistry, Tbilisi State University~ Tbilisi, Georgia. Graduated cum laude

AWARDS / RECOGNITIONS

- 2007 ~ Excellence in Neuroscience Research-Poster PresentationSociety for Neuroscience ~ Louisville Chapter Award

- 2006 ~ Excellence in Neuroscience Research-Poster PresentationSociety for Neuroscience ~ Louisville Chapter Award

- 2002 ~ NATO / Royal Society Postdoctoral Fellowship

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Stipend Covering One-Year Research in United Kingdom

- 1999 ~ PhD Fellowship from Georgian Department of Science and TechnologyGeorgian Presidents Fellowship

- 1996 ~ Graduate Researcher Award-Best Short PresentationGeorgian Academy of Sciences

PUBLICATIONS / ABSTRACTS

Publications

- Jani T, Gobejishvili L, Hote P, Barve A, Joshi-Barve S, Kharebava G, Suttles J, Chen T, Barve S and McClain C. (2009) Inhibition of methionine adenosyltransferase II induces FasL expression, Fas-disc formation and caspase-8 dependent apoptotic death in T leukemic cells. Cell Research 2009 Mar;19(3):358-69

- Kharebava G, Makonchuk D, Kalita K, Zheng JJ, Hetman M. (2008) Requirement of3-phosphoinositide (PDK1) dependent protein kinase for BDNF mediated neuronal survival. J Neurosci. Oct 29;28(44):11409-20

- Szatmari E, Kalita KB, Kharebava G, Hetman M. (2007) Role of kinase suppressorof Ras-1 in neuronal survival signaling by extracellular signal-regulated kinas

e 1/2. J Neurosci. Oct 17;27(42):11389-400- Kalita K, Kharebava G, Zheng JJ, Hetman M. (2006) Role of megakaryoblastic acute leukemia-1 in ERK1/2-dependent stimulation of serum response factor-driven transcription by BDNF or increased synaptic activity. J Neurosci. Sep 27;26(39):10020-32

- Hetman M, Kharebava G. (2006) Survival signaling pathways activated by NMDA receptors. Curr Top Med Chem. 6(8):787-99. Review

- Kharebava G, Habas A1, Szatmari E, Hetman M. (2006) NMDA neuroprotection against a phosphatidylinositol-3 kinase inhibitor, LY294002 by NR2B-mediated suppression of glycogen synthase kinase-3beta-induced apoptosis. J Neurochem. Jan;96(2):

335-48

- Seyfried J, Wang X, Kharebava G, Tournier C. (2005) A novel mitogen-activatedprotein kinase docking site in the N terminus of MEK5alpha organizes the components of the extracellular signal-regulated kinase 5 signaling pathway. Mol Cell Biol Nov;25(22):9820-8

- Javakhishvili N, Tsagareli Z, Sanikidze T, Machavariani M, Bakhutasvili Z, Enukidze M, Kharebava G, Bakhutashvili V. (2001) Antioxidant effect of Plaferon LBduring myocardial infarction in the experiment Experimental and Clinical Medicine 2-3, 11-14

- Chkhikvishvili I, Kharebava G. (2001) Chicoric and Chlorogenic acids in plant

species from Georgia. Applied Biochemistry and Microbiology 37-2, 188-191

- Kharebava G, Garishvili T, Mikeladze D, Bakhutashvili V. (2001) Chromatographic profiles of Plaferon LB. Bull. Georgian Acad. Sci., 163-2, 341-344

- Chkhikvishvili I, Kharebava G. (2001) Quantitative analysis of Chlorogenic acid by HPLC in various grades of apples. Bull. Georgian Acad. Sci. 163-1, 357-360

- Jariashvili T, Kharebava G. (2000) Regulation of Na+,K+-ATPase activity by synaptosomal proteins. Bull. Georgian Acad. Sci. 162 , 558-560

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- Kharebava G, Todadze K, Shanshiashvili L, Zaalishvili E, Sanikidze T, Bakhutashvili A, Lezhava G, Mikeladze D. (1999) Influences of Plaferon-LB, P6 and Metadoxine on the amino acid content, nitrogene oxide biosynthesis and intensity of the EPR signals in the brain of alcoholized rats Proc. Georgian Acad. Sci. Biol. Ser. 25, 77-86

- Kharebava G, Pantsulaia I, Chikovani T, Cheishvili N, Bakhutashvili V. (1999)Regulation of lymphocyte function by peptide factions from Plaferon LB. Proc. Georgian Acad. Sci. Biol. Ser. 25, 87-90

Abstracts

- Kirpich I, Zhang J, Gobejishvili L, Kharebava G, McClain C, Barve S (2010) Binge alcohol exposure inhibits hepatic class I and II histone deacetylases (HDACs): Potential mechanism for alcohol induced liver injury. FASEB Experimental Biology, April 24-28, Anaheim, CA

- Kharebava G, Hetman M. (2007) MAPK pro-survival cascade integrates PDK1 signals to protect trophic deprived cortical neurons. FASEB summer research conferences, Protein kinase and protein phosphorylation, July 7-12, Indian Wels, CA

- Kharebava G, Hetman M. (2006) Critical Role of p90RSK in neuronal survival. Go

rdon research conferences, Growth Factor Signaling, July 16-21, Connecticut College, CT

- Kharebava G, Bakhutashvili V. (2001) Nitric oxide production in rat brain during alcohol loading and withdrawal: influence by peptide P6 purified from human amnion. NATO advanced research workshop Creatine Kinase and Brain Energy Metabolism July 14-18, Tbilisi, Georgia

- Barbakadze T, Kharebava G, Bakhutashvili V, Mikeladze D. (2000) Reduction of NMDA-receptor induced death by placental factor P6. 2nd Convention of Georgian Physiologists, Tbilisi :18-20

- Pantsulaia I, Cheishvili N, Chikovani T, Kharebava G. (1999) Plaferon LB - mod

ulator of the mononuclear cell functional activity. Young Scientists Presidential Scholarship Grantees of Georgia, TRANSACTIONS-I, Tbilisi, 1, 15-18

- Bakhutashvili V, Gagua M, Garishvili T, Gelashvili L, Kharebava G, Kvaratskhelia E. (1997) Human placenta antioxidant compounds of peptide nature. 11th International Symposium on Atherosclerosis. Paris, October, 134, 199