Science Learning Center Chemistry Pre-laboratory Tutorials Written By Patrick Herklotz

Science Learning Center

Chemistry Pre-laboratory Tutorials

Written By Patrick Herklotz

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Tutorial 3 Calibrating the Vernier Colorimeter Sensor and Recording Data Using

this Sensor

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VERNIER

LabPro

Quick SetupStart/StopTransfer

Vernier

Power Supply

STAT PLOT F1 TBLSET F2 FORNAT F3 CALC F4 TABLE F5

Alpha

2ND Mode DEL

STAT

PRGM

COS

(

X,T,O,N

APPSMATH

7

4

1

0

8

5

2

.

9

6

3

(-)

x

+

-

÷

Enter

X -1

X2

LOG

LN

STO>

ON

SIN

,

VARS CLEAR

TAN

>

)

Step 1: Assemble the LabPro Calculator as explained in Tutorial 1

Top View

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VERNIER

LabPro


Vernier

Power Supply


Alpha

2ND Mode DEL

STAT

PRGM

COS

(

X,T,O,N

APPSMATH

7

4

1

0

8

5

2

.

9

6

3

(-)

x

+

-

÷

Enter

X -1

X2

LOG

LN

STO>

ON

SIN

,

VARS CLEAR

TAN

>

)

Step 1: Assemble the LabPro Calculator as explained in Tutorial 1

Top View

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VERNIER

LabPro


Vernier

Power Supply


Alpha

2ND Mode DEL

STAT

PRGM

COS

(

X,T,O,N

APPSMATH

7

4

1

0

8

5

2

.

9

6

3

(-)

x

+

-

÷

Enter

X -1

X2

LOG

LN

STO>

ON

SIN

,

VARS CLEAR

TAN

>

)

Side View

Channel 1 Channel 2 Channel 3 Channel 4

Step 2: Obtain the colorimeter sensor and plug it into channel 1. You must run the DataMate program to turn the colorimeter on. The colorimeter will measure absorbance in a default wavelength of 470nm. A green light illuminates to signify which wavelength is selected. Allow the colorimeter to warm up for 5 minutes before beginning any experiment.

CAL

430nm

470nm 565nm

635nm

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VERNIER

LabPro


Vernier

Power Supply


Alpha

2ND Mode DEL

STAT

PRGM

COS

(

X,T,O,N

APPSMATH

7

4

1

0

8

5

2

.

9

6

3

(-)

x

+

-

÷

Enter

X -1

X2

LOG

LN

STO>

ON

SIN

,

VARS CLEAR

TAN

>

)

Step 2: Obtain the colorimeter sensor and plug it into channel 1. You must run the DataMate program to turn the colorimeter on. The colorimeter will measure absorbance in a default wavelength of 470nm. A green light illuminates to signify which wavelength is selected. Allow the colorimeter to warm up for 5 minutes before beginning any experiment.

Side View

Channel 1 Channel 2 Channel 3 Channel 4

Channel 1 Port

click

VERNIER SOFTWARE DATAMATE

(VER 1.15)

ROM 6.27 (C)2003

Checking Sensors

Mode: TIME GRAPH-180

1: SETUP 4: ANALYZE2: START 5: TOOLS3: GRAPH 6: QUIT

CH 1: Absorbance 0.475



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VERNIER

LabPro


Vernier

Power Supply


Alpha

2ND Mode DEL

STAT

PRGM

COS

(

X,T,O,N

APPSMATH

7

4

1

0

8

5

2

.

9

6

3

(-)

x

+

-

÷

Enter

X -1

X2

LOG

LN

STO>

ON

SIN

,

VARS CLEAR

TAN

>

)

CAL

430nm

470nm

Step 3: Fill a Cuvette full of distilled water and place it into the colorimeter. Be sure that the clear side of the Cuvette is facing forward and backward in the colorimeter and the striated sides face to the side. Push the > key on the colorimeter and hold it down until the green light changes to 565nm. This signifies that this new wavelength has been selected.

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VERNIER

LabPro


Vernier

Power Supply


Alpha

2ND Mode DEL

STAT

PRGM

COS

(

X,T,O,N

APPSMATH

7

4

1

0

8

5

2

.

9

6

3

(-)

x

+

-

÷

Enter

X -1

X2

LOG

LN

STO>

ON

SIN

,

VARS CLEAR

TAN

>

)

Step 3: Fill a Cuvette full of distilled water and place it into the colorimeter. Be sure that the clear side of the Cuvette is facing forward and backward in the colorimeter and the striated sides face to the side. Push the > key on the colorimeter and hold it down until the green light changes to 565nm. This signifies that this new wavelength has been selected.

CAL

430nm

470nm 565nm

635nm

CAL

430nm

470nm 565nm

635nm

Top View

Previous Slide

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VERNIER

LabPro


Vernier

Power Supply


Alpha

2ND Mode DEL

STAT

PRGM

COS

(

X,T,O,N

APPSMATH

7

4

1

0

8

5

2

.

9

6

3

(-)

x

+

-

÷

Enter

X -1

X2

LOG

LN

STO>

ON

SIN

,

VARS CLEAR

TAN

>

)

TI-84 Silver EditionTexas Instruments




Step 4: To calibrate the colorimeter, press the 1 key (SETUP). In the next menu, press the 3 key (ZERO). In the third menu press the 2 key (Zero ALL CHANNELS). In the final menu, press the ENTER key.

CAL

430nm

470nm 565nm

635nm

Top View

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VERNIER

LabPro


Vernier

Power Supply


Alpha

2ND Mode DEL

STAT

PRGM

COS

(

X,T,O,N

APPSMATH

7

4

1

0

8

5

2

.

9

6

3

(-)

x

+

-

÷

Enter

X -1

X2

LOG

LN

STO>

ON

SIN

,

VARS CLEAR

TAN

>

)

Step 4: To calibrate the colorimeter, press the 1 key (SETUP). In the next menu, press the 3 key (ZERO). In the third menu press the 2 key (Zero ALL CHANNELS). In the final menu, press the ENTER key.





CH 1: ColorimeterCH 2:CH 3:CH 4:DIG1:DIG2:MODE: TIME GRAPH-900

1:OK 3:ZERO2:CALIBRATE 4:SAVE/LOAD

SELECT CHANNEL1: CH1-ABSORBANCE2: ALL CHANNELS

CH 1: ABSORBANCE -0.135

PRESS [ENTER] TO ZERO

CH 1: ABSORBANCE 0



Colorimeter is now Calibrated!

CAL

430nm

470nm 565nm

635nm

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VERNIER

LabPro


Vernier

Power Supply


Alpha

2ND Mode DEL

STAT

PRGM

COS

(

X,T,O,N

APPSMATH

7

4

1

0

8

5

2

.

9

6

3

(-)

x

+

-

÷

Enter

X -1

X2

LOG

LN

STO>

ON

SIN

,

VARS CLEAR

TAN

>

)


CH 1: ABSORBANCECH 2:CH 3:CH 4:DIG1:DIG2:MODE: TIME GRAPH-180


Step 5: Change the Data Collection mode first by pressing the 1 key (SETUP). In the next menu, use the “down key” to scroll the cursor to the left of MODE; press the ENTER key. In the SELECT MODE menu, press the 3 key to select EVENTS WITH ENTRY. Press 1 in the final menu to go back to the default screen.

CAL

430nm

470nm 565nm

635nm

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VERNIER

LabPro


Vernier

Power Supply


Alpha

2ND Mode DEL

STAT

PRGM

COS

(

X,T,O,N

APPSMATH

7

4

1

0

8

5

2

.

9

6

3

(-)

x

+

-

÷

Enter

X -1

X2

LOG

LN

STO>

ON

SIN

,

VARS CLEAR

TAN

>

)

Step 5: Change the Data Collection mode first by pressing the 1 key (SETUP). In the next menu, use the “down key” to scroll the cursor to the left of MODE; press the ENTER key. In the SELECT MODE menu, press the 3 key to select EVENTS WITH ENTRY. Press 1 in the final menu to go back to the default screen.







SELECT MODE1:LOGDATA2:TIME GRAPH3:EVENTS WITH ENTRY4:SINGLE POINT5:SELECTED EVENTS6:RETURN TO SETUP SCREEN



CH 1: ABSORBANCE 0

Mode: EVENTS WITH ENTRY


CAL

430nm

470nm 565nm

635nm

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VERNIER

LabPro


Vernier

Power Supply


Alpha

2ND Mode DEL

STAT

PRGM

COS

(

X,T,O,N

APPSMATH

7

4

1

0

8

5

2

.

9

6

3

(-)

x

+

-

÷

Enter

X -1

X2

LOG

LN

STO>

ON

SIN

,

VARS CLEAR

TAN

>

)


CAL

430nm

470nm 565nm

635nm

Cuvette A

CH 1: ABSORBANCE 0



Step 6: Begin collecting data by pressing the 2 key (START) in the default menu. Be sure to use a chemwipe to get all the fingerprints off the outside of the cuvette you are using. Place cuvette A into the colorimeter. Give the Colorimeter 15 seconds to begin taking accurate readings.

CAL

430nm

470nm 565nm

635nm

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VERNIER

LabPro


Vernier

Power Supply


Alpha

2ND Mode DEL

STAT

PRGM

COS

(

X,T,O,N

APPSMATH

7

4

1

0

8

5

2

.

9

6

3

(-)

x

+

-

÷

Enter

X -1

X2

LOG

LN

STO>

ON

SIN

,

VARS CLEAR

TAN

>

)

Step 6: Begin collecting data by pressing the 2 key (START) in the default menu. Be sure to use a chemwipe to get all the fingerprints off the outside of the cuvette you are using. Place cuvette A into the colorimeter. Give the Colorimeter 15 seconds to begin taking accurate readings.


CAL

430nm

470nm 565nm

635nm

Cuvette A

CH 1: ABSORBANCE 0



PRESS [ENTER] TO COLLECTOR [STO] TO STOP

1 0.052

CAL

430nm

470nm 565nm

635nm

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VERNIER

LabPro


Vernier

Power Supply


Alpha

2ND Mode DEL

STAT

PRGM

COS

(

X,T,O,N

APPSMATH

7

4

1

0

8

5

2

.

9

6

3

(-)

x

+

-

÷

Enter

X -1

X2

LOG

LN

STO>

ON

SIN

,

VARS CLEAR

TAN

>

)

Step 7: After 15 seconds press the ENTER key. In the next menu, you will be prompted to “ENTER VALUE?” Type in the concentration of the substance in cuvette A and press the ENTER key. Pull Cuvette A out and repeat steps 6 and 7 for the rest of the substances in separate cuvettes.


CAL

430nm

470nm 565nm

635nm


1 0.052

CAL

430nm

470nm 565nm

635nm

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VERNIER

LabPro


Vernier

Power Supply


Alpha

2ND Mode DEL

STAT

PRGM

COS

(

X,T,O,N

APPSMATH

7

4

1

0

8

5

2

.

9

6

3

(-)

x

+

-

÷

Enter

X -1

X2

LOG

LN

STO>

ON

SIN

,

VARS CLEAR

TAN

>

)

Step 7: After 15 seconds press the ENTER key. In the next menu, you will be prompted to “ENTER VALUE?” Type in the concentration of the substance in cuvette A and press the ENTER key. Pull Cuvette A out and repeat steps 6 and 7 for the rest of the substances in separate cuvettes.


CAL

430nm

470nm 565nm

635nm


1 0.052

ENTER VALUE?PRESS [ENTER] TO COLLECTOR [STO] TO STOP

2 0.012

CAL

430nm

470nm 565nm

635nm

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VERNIER

LabPro


Vernier

Power Supply


Alpha

2ND Mode DEL

STAT

PRGM

COS

(

X,T,O,N

APPSMATH

7

4

1

0

8

5

2

.

9

6

3

(-)

x

+

-

÷

Enter

X -1

X2

LOG

LN

STO>

ON

SIN

,

VARS CLEAR

TAN

>

)


CAL

430nm

470nm 565nm

635nm


4 0.00025

Step 7: Once you have recorded the absorbance of the substances in cuvette A, B, and C using steps 6 and 7, press the STO button to end recording additional measurements. A stat plot of the recorded absorbencies will appear on the screen. Press ENTER to escape this screen and return to the default menu.

CAL

430nm

470nm 565nm

635nm

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VERNIER

LabPro


Vernier

Power Supply


Alpha

2ND Mode DEL

STAT

PRGM

COS

(

X,T,O,N

APPSMATH

7

4

1

0

8

5

2

.

9

6

3

(-)

x

+

-

÷

Enter

X -1

X2

LOG

LN

STO>

ON

SIN

,

VARS CLEAR

TAN

>

)

Step 7: Once you have recorded the absorbance of the substances in cuvette A, B, and C using steps 6 and 7, press the STO button to end recording additional measurements. A stat plot of the recorded absorbencies will appear on the screen. Press ENTER to escape this screen and return to the default menu.


CAL

430nm

470nm 565nm

635nm


4 0.00025

ABSORBANCE P1

X=concentration # y=Absorbance

CH 1: ABSORBANCE 0.009



CAL

430nm

470nm 565nm

635nm

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VERNIER

LabPro


Vernier

Power Supply


Alpha

2ND Mode DEL

STAT

PRGM

COS

(

X,T,O,N

APPSMATH

7

4

1

0

8

5

2

.

9

6

3

(-)

x

+

-

÷

Enter

X -1

X2

LOG

LN

STO>

ON

SIN

,

VARS CLEAR

TAN

>

)

Step 8: Press the 6 key (QUIT) then the ENTER key to save your recorded concentration vs. absorbance data in L1 and L2. The data can be downloaded the into the LoggerPro program as described in tutorial 2.


CAL

430nm

470nm 565nm

635nm




CAL

430nm

470nm 565nm

635nm

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VERNIER

LabPro


Vernier

Power Supply


Alpha

2ND Mode DEL

STAT

PRGM

COS

(

X,T,O,N

APPSMATH

7

4

1

0

8

5

2

.

9

6

3

(-)

x

+

-

÷

Enter

X -1

X2

LOG

LN

STO>

ON

SIN

,

VARS CLEAR

TAN

>

)

Step 8: Press the 6 key (QUIT) then the ENTER key to save your recorded concentration vs. absorbance data in L1 and L2. The data can be downloaded the into the LoggerPro program as described in tutorial 2.


CAL

430nm

470nm 565nm

635nm




EVENTS IN L1CH1 IN L2CH2 IN L3CH3 IN L4CH4 IN L5D IN L6, V In L7A IN L8 [ENTER]

End of Tutorial 3

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Science Learning Center Chemistry Pre-laboratory Tutorials Written By Patrick Herklotz