Science Brief: Enzyme Nutrition for Kids Lisa Hudson, RDN • page 1

Transformation Enzyme Corporation (TEC) is a nutritional supplement company specializing in the development of high quality enzyme-based products for the health care professional. Through clinical and observational research, our efforts focus on teaching the importance of nutrient ac-quisition as the foundation of wellness and healthy living.

A poor diet and a digestive system that fails to process food for bioavailability and absorption will undermine the body’s

coping ability and create conditions favorable for disease and metabolic disorders. Based on this fact, we strive to educate health care professionals worldwide on the benefits of a balanced diet and proper digestion. The primary benefits of supplementation with digestive enzymes are:

1. A healthy and optimally functioning digestive system

2. Enhanced bioavailability of nutrients for cellular vitality and healthy immune system

3. Efficient and timely removal of metabolic waste and environmental toxins

Historically, supplemental digestive enzymes were obtained from the pancreatic juice of animals, commonly bovine and porcine. However, in the past several decades, enzymes derived from non-animal sources have gained increased recognition. Plant enzymes such as bromelain and papain are being commonly used. More current research suggests mycelial enzymes from Aspergillus oryzae and Aspergillus niger as well as other microorganisms have been proven safe and effective, and often times clinically superior to traditional “animal” and “plant” enzymes for digestion.

Science Brief:Improved Digestion for Children with Innovative Enzyme Nutrition Solutions

The demand has never been greater for enzyme-based dietary supplements supporting improved digestion for children. Transformation™ presents its Zymes 4 Kidz product line in powdered form and as berry-flavored chewables.

Transformation’s scientific staff with decades of combined experience in biochemistry, nutrition, and cellular and mo-lecular biology has designed a new formula to assist infants and children in the digestion of proteins, carbohydrates and fats. This Science Brief will review the safety of and growing need for supplemental digestive enzymes for our children. It will also highlight the unique benefits of Transformation’s Kidz Digest digestive formulas.

The Need for Good Nutrition and, More Importantly, Optimal DigestionThe health of our children is declining at an alarming rate. Autism now affects 1 in 63 girls and 1 in 38 boys (Hertz-Pic-ciotto and Delwiche 2009). Allergies are reported at a rate of 1 in 4 children, asthma 1 in 11, and ADHD is now present in 1 out of every 3 children. Furthermore, the incidence of adult lifestyle diseases such as GERD, obesity, diabetes, and cancer are also on the rise in the younger populations.

These increases can be attributed to factors such as poor diet (specifically in the form of processed foods, commercial formulas, and Genetically Modified Organisms / GMO’s), improper digestion, and the prevalence of toxins in the envi-ronment. While genetics may play a part, there is more and more evidence pointing to the fact that the vast majority of all disease can be traced back to poor diet, mal-digestion, and the inability of the gut to function properly.

In our clinical practices, we are seeing more and more food-related immune responses. These food intolerances or sensitivities arise from undigested food molecules. These unrecognized “chemicals” 1) damage the cells in the lining of the small intestines, 2) increase the permeability of the gut wall allowing undigested food molecules to “leak” into the blood, and 3) trigger the immune cells to respond to these “chemicals” as antigens. This immune response may manifest anywhere in the body and in many different forms, and that is what brings the patient to seek help.

“All disease begins in the gut” -Hippocrates (ca. 460 BC - ca. 370 BC)

Science Brief: Enzyme Nutrition for Kids Lisa Hudson, RDN • page 2

While traditional medicine treats the symptoms with immuno-suppressive drugs, the more natural approach is to heal the intestinal lining with probiotics, L-Glutamine, and various nutritional supplements. But isn’t the root of the problem diet and undigested food molecules? Shouldn’t the first line of defense be to improve food choices and support digestion, especially those allergy-prone foods found in so many of the processed and convenience foods that are available to our children today?

Let’s focus on the two variables we can have an immediate and positive impact on: food choices and digestion. These can be addressed easily by paying attention to what we feed our families and by supplementing with digestive enzymes. Choosing organic, non-GMO, fresh, whole foods and avoiding fast/convenience foods laden with chemicals, preservatives, and sugar is the first place to start.

The health benefits of a healthy diet coupled with supple-mental enzymes include:

• Bioavailability of valuable nutrients to the cell- Carbohydrates - the body’s main energy source- Fats - supply fatty acids for energy and for building cell

walls, cholesterol, and hormones- Proteins - supply amino acids which are the structural

components for every cell, tissue, muscle, and organ and the building blocks of our immune system, neuro-transmitters, and hormones

- Vitamins and Minerals - key to all processes taking place in the body and co-factors for many biochemical reactions

• Efficient, appropriate, and healthy immune function• Maintenance of a healthy intestinal lining• Timely elimination of waste for decreased toxicity

When the body is completely digesting nutritious foods, the nutrients are made available to the cells, thus the opportunity for achieving true health and wellness is present.

Kidz Digest: Formula Rationale According to the CDC, childhood obesity has more than tripled in the past 30 years, and with obesity comes the rise in many other health concerns including diabetes, CVD, hormone imbalances, and various cancers. The CDC also lists healthy eating as one of the top preventative measures against childhood obesity. However, with the prevalence of processed and GMO-ridden foods in our children’s diets, from infant formulas to cereals, our sources for healthy eat-ing have narrowed. What’s more, with daily environmental stressors we face, our digestive systems are often too taxed to function optimally.

According to Dr. DicQie Fuller-Looney, genetically modify-ing our foods create changes in the protein structure of that food. She teaches that no one can be assured these protein potentials can be controlled once they are part of the system. Dr. Fuller’s concern is that these “proteins” can send mixed signals which can lead to the serious health issues we are witnessing in our children. Processed foods in our children’s diet create under-nourishment due to the lack of bio-avail-able vitamins and minerals in these foods. Our children are more susceptible because they are still developing their gut

“Tummy Troubles”There truly is no substitute for breastfeeding. However, there are circum-stances that can and do prevent someone from breastfeeding. The ingredi-ents in most formulas are synthetic. The first three ingredients are protein (usually from cow’s milk or soy, which we also know is from a genetically modified source 80% of the time), oil (usually a blend beginning with palm oil), and sugar (usually from corn).

It is therefore not hard to build a case for enzyme supplementation for for-mula fed babies. You can see right away why an infant would have tummy troubles. Hard-to-digest proteins, bad fats, and GMO corn sugar. You won’t find “sugar” listed on the label either but according to an investigative article by NBC Chicago News they found anywhere from 4 to 13 grams per serving. That could look like 1 teaspoon per 5 ounces. A rule of thumb is a baby typi-cally drinks 2.5 ounces of formula per pound of body weight each day. So if a baby weighs 10 pounds, they would consume 5 teaspoon of sugar per day.www.nbcchicago.com/news/health/target-5-sugar-baby-formula-139339308.html

Science Brief: Enzyme Nutrition for Kids Lisa Hudson, RDN • page 3

flora as well as their digestive, endocrine, and reproductive systems.

With all of these factors affecting the health of our children, it’s clear the need has never been greater for Transforma-tion’s new Zymes 4 Kidz children’s line to support improved digestion for the health of our children.*

Supporting more complete digestion of nutrients from breast milk, formula, or solid food not only supports proper de-velopment of our growing children but also helps alleviate symptoms of poor digestion like gas, cramping, bloating, acid indigestion, and allergies. That is why Transformation™

created Kidz Digest to assist with a child’s complete digestion of proteins, carbohydrates, and fats.*

Our new children’s digestive formula is available in two forms. The powdered formulation for infants can be given before each feeding and in between feedings if needed for systemic health. And whereas the powdered form allows for convenient delivery for infants, Kidz Digest is also available as a berry flavored chewable for toddlers through young adults.

Kidz Digest: Ingredient Highlights Kidz Digest includes digestive enzymes from microbial fungal sources that are safe for children, effective, and GI tract stable

(Borowitz 2012, Graham 1984, Mamadou 2005, Medhekar 2004). This means the digestive enzymes in Kidz Digest do not require an enteric coating to survive the environment of the stomach but rather initiate digestion in the stomach and continue the digestive process in the small intestines.

This is a key point because it allows maximum time for the supplemental enzymes to assist the endogenous enzymes to complete the breakdown of the carbohydrates and pro-teins that may be triggering immune reactions. Furthermore, there is no negative feedback or inhibition of the pancreas associated with taking supplemental enzymes. This is be-cause secretion of pancreatic enzymes is regulated by the presence of hormones (secretin and cholecystikinin) and not the presence of enzymes.

Proteolytic EnzymesOne of the most common sources of allergies is protein, whether the source is from food or the environment. Incom-plete digestion of proteins not only leads to allergic reactions that manifest in many ways but also compromises bio-avail-ability of amino acids and nutrients essential for children who are still growing and developing. Dipeptidyl Peptidase IV (DPP-IV) is a proteolytic enzyme that hydrolyses the strong proline and glutamine bonds in gluten, a wheat protein. Casein is another protein similar to gluten, found in human

Did You Know?• According to the Centers for Disease Control and Prevention,

if 4 million children are born in the United States every year, approximately 36,500 children will eventually be diagnosed with an Autism Spectrum Disorder. Assuming the prevalence rate has been constant over the past two decades, we can estimate that about 730,000 individuals between the ages of 0 to 21 have an ASD. (www.cdc.gov/ncbddd/autism/data)

• Dr. James Braly, allergist and immunologist, estimates the incidence of gluten sensitivity to be around 30% of the U.S. population. Dr. Fine of Enterolab, based on results of IgA antibody tests and gene tests from his laboratory, estimates 30-40%. However, as some people have no symptoms, so are never tested, some commentators feel that the actual number is closer to 50%.

• Celiac disease is an autoimmune digestive disease that dam-ages the villi of the small intestine and interferes with absorp-tion of nutrients from food. An estimated 2.8 million Americans are diagnosed with celiac disease while an additional 95% of celiacs are undiagnosed. The celiac disease diagnosis rate may reach 50-60% by 2019. (www.celiaccentral.org)

Science Brief: Enzyme Nutrition for Kids Lisa Hudson, RDN • page 4

milk (25-40%) and cow’s milk (80%). Incomplete digestion of proteins found in foods containing wheat and dairy are linked to food intolerances.

DPP-IV is considered an exo-protease, meaning it cleaves mainly the terminal bonds on an amino acid chain. It is this “exo” property that assists our endogenous enzymes and makes DPP-IV especially affec-tive in the breakdown of proteins that are more difficult to digest.

The DPP-IV in Kidz Digest is obtained from the controlled fermentation of select Asperig-illus species and is effective in a pH of 2.0-8.0 making it ideal to support protein digestion in the stomach and small intes-tines. This product contains 300 DPP-IV per serving which has been found effective in clinical settings.

Additionally, the protease blend in Kidz Digest includes acid, neutral, alkaline endo and exo peptidases. This comprehensive proteolytic enzyme blend assists in the digestion of all dietary proteins. The proteases work in a broad pH range making them effective for digestion in the stomach and small intestines.

Complete protein digestion is important for everyone, not just those suffering from food intolerances. The proteolytic

enzymes in this formula support the digestion of proteins to help produce amino acids, which are important building blocks in the human body. These amino acids are used to build muscles, metabolic enzymes, neurotransmitters, and many other essential biochemicals. Proteolytic enzyme supplementation can therefore be essential to maintaining

overall health as it promotes max-imum nutrition from the protein consumed in a typical diet.

CarbohydrasesKidz Digest includes a com-prehensive blend of carbohy-drases that work synergistically for maximum digestion of all carbohydrates – starch, fiber, and sugar.* This is ideal for those children who may have been given formula, cereal, or commercially prepared baby food before their GI tracts were fully developed and capable of handling these carbohydrates.

Specifically, the enzymes al-pha-galactosidase, glucoamylase, amylase, and diastase support digestion of complex carbohydrates. Invertase and lactase support digestion of sugars. In addition, the enzymes pectinase, phytase, cellulase, and hemicellulase break down fiber and promote the release of vital nutrients often “locked” within these chemical structures. This does not, however, negatively impact or diminish the presence of

Hospitals Bank ‘Liquid Gold’: Human Breast MilkBreast feeding, while ideal, is not always possible, and the need for sup-port in this area is growing. Texas Children’s Hospital in Houston, TX, is the first to offer an actual donor milk bank to support infants. “The science is pretty strong that human milk is best for human babies,” said Neona-tologist Dr. Michael Speer, Professor of Pediatrics at Baylor College of Medicine.

Transformation agrees with Dr. Speer and is excited to see the develop-ment of such programs, but this alone is not enough to reach every child that may not be getting the nutritional support breast milk supplies. That is why supplementing with digestive enzymes and probiotics is so vital, especially for infants living on a formula diet.

http://www.npr.org/blogs/health/2012/08/23/159925707/hospitals-bank-liquid-gold-human-breast-milk

Kidz Digest™ was created to assist children with maximum digestion of nutrients.* This berry-flavored chewable will assist your child’s digestion of proteins, carbohydrates, and fats.* Supporting a more complete digestion and assimilation of nutrients helps to promote overall health and wellbeing.*Transformation™ advocates healthy food choices for children.RECOMMENDED USAGE: Two (2) tablets with every meal or as directed by your healthcare professional.Enzyme activity is measured in Food Chemicals Codex (FCC) units.Store tightly sealed in a cool, dry place. Keep out of reach of children.

ManuFaCTurEd For TranSForMaTion EnzyME Corp., 2900 WilCrEST dr., SuiTE 220, HousTon, TX 77042

*THESE STaTEMEnTS HavE noT bEEn EvaluaTEd by THE Food and drug adMiniSTraTion. THis produCT iS noT inTEndEd To diagnoSE, TrEaT, CurE, or prEvEnT any diSEaSE. oTHEr ingrEdiEnTs: FruCToSE, XyliTol, naTural MiXEd bErry Flavor, naTural sTraWBErry Flavor, MagnESiuM STEaraTE, SiliCa30 TableTs

Total Carbohydrates <1 g <1%† Sugar <1 g ** Sugar alcohols <1 g ** peptidase blend (50,000 HuT and 300 dpp-iv) 114 mg ** polysaccharolytic blend 90 mg **amylase, glucoamylase, lactase, alpha-galctosidase, pectinase, diastase, invertase, cellulase, hemicellulaseFlax Seed

30 mg **lipase (3,000 Fip) 11 mg **bifidobacterium infantis 2 mg **† based on a 2,000 calorie diet**daily value not established

amount per Serving % daily value

Serving Size 2 Chewable Tablets Servings per Container 15

Supplement Facts

Kidz4Zymes™*Kidz Digest

EnzymeSupplement

Berry Flavor Chewable

Breast milk is the best source of nutrition for a child’s healthy

growth and development. Transformation™ created Kidz Digest™

because we understand there are circumstances that prevent

breastfeeding. Whether breast fed or formula fed, this formulation

assists with digestion to alleviate symptoms of occasional gas and

bloating.* All Transformation™ formulas are carefully prepared to

assure maximum quality and nutritional effectiveness.*

Transformation™ advocates breastfeeding as the optimum

source of nutrition for infants.

ABSOLUTELY NO FILLERS

RECOMMENDED USAGE: 1/8 tsp (1/2 scoop) mixed in a spoon

of tepid water or placed on the tongue or as directed by your

healthcare professional. Do not add to a bottle of baby formula

or mix in baby food because it will break down the food in the

container making it less palatable.

Enzyme activity is measured in Food Chemicals Codex (FCC) units.

Store tightly sealed in a cool, dry place. Keep out of reach of children.

MAnuFACTurED For TrAnSForMATion

EnzyME Corp., 2900 WilCrEST Dr.,

SuiTE 220, HouSTon, TX 77042

*THESE STATEMEnTS HAvE noT BEEn

EvAluATED By THE FooD AnD Drug

ADMiniSTrATion. THiS proDuCT iS

noT inTEnDED To DiAgnoSE, TrEAT,

CurE, or prEvEnT Any DiSEASE.Net weight 41.5g (1.46 oz)

Kidz4Zymes™*Kidz Digest

EnzymeSupplement

PowderThIS pRODUCT hAS NO ADDED SUGAR

OR ARTIFICIAL COLORS

polysaccharolytic Blend 131 mg †

Amylase, glucoamylase, alpha-galctosidase,

invertase, lactase, pectinase, diastase, phytase,

cellulase, hemicellulase

peptidase Blend (50,000 HuT and 300 Dpp-iv) 119 mg †

lipase (3,000 Fip) 53 mg †

Flax Seed30 mg †

Bifidobacterium infantis 1 mg †

† Daily value not established

Amount per Serving % Daily value

Serving Size 332.7 mg (app 1/8 tsp)

Servings per Container about 125

Supplement Facts

Science Brief: Enzyme Nutrition for Kids Lisa Hudson, RDN • page 5

fiber necessary for proper elimination of wastes. All of the enzymes in Kidz Digest work synergistically with endogenous enzymes and are vital for children suffering from allergies or digestive discomfort such as gas, cramping, bloating, reflux, constipation, or diarrhea.*

The proprietary carbohydrase blend is acid stable and works in a wide pH range, therefore functioning throughout the gas-trointestinal tract. Kidz Digest is a perfect solution for those babies who must be fed commercial formulas as well as the toddler as he or she begins receiving solid foods.*

Lipase Kidz Digest also includes 3,000 FIP (lipase units) for complete digestion of fats.* Breast milk is high in fat to support the babies energy and developmental needs. Proper digestion and assimilation of fats, essential fatty acids, and fat soluble vitamins is crucial for the development of the brain, nervous system, and cellular membrane integrity.

ProbioticsKidz Digest includes the probiotic Bifidobacterium infantis (strain ATCC 15697). They were first identified over 100 years ago from human feces and were quickly associated with a healthy gastrointestinal tract due to their numerical dominance in breast fed infants compared to bottle fed in-fants. “In the neonate their numerical advantage confers a substantial health benefit by hindering pathogen colonization through competitive exclusion. It is important for establishing and maintaining homeostasis of the intestinal ecosystem to allow for normal digestion” (Haros et al, 2007). B. Infantis is a leading probiotic due to the vast number of studies which provide a growing body of evidence for its role in a myriad potential health benefits.

In the 2007 study referenced above, it was shown that B. infantis ATCC 15697 contributed to the hydrolysis of phytic acid which is a component in carbohydrates often found difficult to digest, thus supporting those who are challenged with the digestion of wheat and other grains. Additionally, probiotic organisms produce digestive enzymes which act to support the endogenous enzymes of the host. Probiotic bacteria are known to produce lactases, proteases, amy-lases, and sucrases.

Animal studies show that the enzymes produced by probiot-ics play important roles in the digestive tracts of mammals. Laboratory animals lacking a healthy colony of flora in their gut ecology have significantly reduced intestinal activities of these essential enzymes, and as a result do not show the same health and productivity as animals with normal gut flora. Studies show that supplementation of probiotic organisms can improve these enzyme activities as well as the animal’s health and productivity. Similar results have been noted in humans.

Kidz DigestChewable

*

Kidz DigestPowder

*

Amount Per Serving % Daily ValueTotal Carbohydrates <1 g <1%† Sugar <1 g ** Sugar alcohols <1 g **Peptidase Blend (50,000 HUT and 300 DPP-IV) 114 mg ** Polysaccharolytic Blend 90 mg ** Amylase, glucoamylase, lactase, alpha-galctosidase, pectinase, diastase, invertase, cellulase, hemicellulaseFlax Seed 30 mg ** Lipase (3,000 FIP) 11 mg **Bifidobacterium infantis 2 mg **† Based on a 2,000 calorie diet**Daily Value not established

Supplement FactsServing Size: 2 Chewable TabletsServings Per Container: 15/45/90

Other Ingredients: Fructose, Vegetable Cellulose, Granular Xylitol, Natural Mixed Berry Flavor, Natural Strawberry Flavor, Magnesium Stearate, Silica

Amount Per Serving % Daily ValuePolysaccharolytic Blend 131 mg † Amylase, glucoamylase, alpha-galctosidase, invertase, lactase, pectinase, diastase, phytase, cellulase, hemicellulase

Peptidase Blend 119 mg † Protease 50,000 HUT Dipeptidyl peptidase IV 300 DPP-IV

Lipase (3,000 FIP) 53 mg †Flax Seed 30 mg † Bifidobacterium infantis 1 mg †

† Daily Value not established

Supplement FactsServing Size: 332.7 mg (app 1/8 tsp)Servings Per Container: about 125

This product has no added sugar or artificial colors

Science Brief: Enzyme Nutrition for Kids Lisa Hudson, RDN • page 6

Dietary IngredientsWhen delivering supplemental enzymes in a powdered form, a “de-dusting” agent such as maltodextrin is often used. How-ever, maltodextrin can be problematic, especially for those with digestive concerns. Transformation™ has chosen to use a small amount of flax seed, a more nutritious ingredient, to serve as the de-dusting agent in our powdered formula. Flax seed is a balanced source of omega fatty acids and has traditionally been used for colon conditions, gastritis, bladder problems, and inflammation.

Transformation™ has always stood firm on the use of no “bind-ers, fillers, or excipients” and takes pride in having the purest enzyme products on the market. When delivering enzymes in a chewable form additional ingredients are necessary. In the chewable tablets we use the smallest amount of ingredients necessary in order to provide a high quality enzyme product to children who otherwise will not take enzymes.

SummarySince 1991, Transformation™ has worked closely with health professionals to provide high quality enzyme-based supplements designed to support more effective acquisition of essential nutrients through improved digestion. Trans-formation’s founder and principal formulator, Dr. DicQie Fuller-Looney, has over thirty years of specialized clinical experience observing and working with individuals with many digestive concerns.

Transformation™ advocates breastfeeding as the optimum source of nutrition for infants. We believe breast milk is the best source of nutrition for a child’s healthy growth and development. Transformation™ created powdered Kidz Di-gest because we understand breast milk may sometimes be difficult to digest, but also there are circumstances that prevent breastfeeding. Whether breast fed or formula fed, this formulation assists with digestion to alleviate symptoms

Should children take enzymes?In order to make sure your child gets proper nutrient acquisition, you will want to promote optimal diges-tion, a healthy immune system, and a healthy GI tract with proper elimination. Therefore, if your general philosophy towards health is to prevent and be proactive, you should definitely give your child enzymes. Transformation’s clinic has also successfully applied enzyme protocols with various digestive disorders such as diarrhea, constipation, colic, reflux, allergies, eczema, asthma, cystic fibrosis, and autism, to name just a few.

How do I give the digestive enzymes to a baby?When breastfeeding, we first suggest that the mother take the basic enzyme protocol – a digestive formula, a proteolytic formula, and a probiotic – to ensure that her digestion is optimal. Many times this calms the baby’s digestive system as well. To support the baby’s digestion still needs assistance, you can easily and safely administer enzymes. Simply mix the Kidz Digest™ powder in a small amount of tepid water (approximately 1cc) and, using a syringe or medicine dropper, give it to the baby before the normal feeding. At times, it may be difficult to give the enzymes prior to feeding. If this is the case, you may give the enzymes to the baby after breastfeeding.

If the baby is being formula-fed, the enzymes should be mixed with 1cc of tepid water and given just be-fore feeding, as described above. Do not mix the Kidz Digest™ powder with formula in the bottle, as it will begin digesting the formula immediately. If it is difficult to give the enzymes prior to feeding, the enzymes may be given after feeding.

Are herbs recommended for children?Transformation™ does have licensed health care professionals who choose to give several other Trans-formation™ products to their younger patients, i.e., those formulations containing herbs. However, our general philosophy is to keep it simple with the basic protocol of enzymes and probiotics only. If you have a question about any specific ingredients, please contact our Technical Support staff.

Science Brief: Enzyme Nutrition for Kids Lisa Hudson, RDN • page 7

of digestive discomfort and allergies.*

Kidz Digest in chewable tablet form was created to assist children of all ages with maximum digestion of nutrients.* This berry-flavored chewable will assist your child’s digestion of proteins, carbohydrates, and fats.* Supporting complete digestion and assimilation of nutrients helps to promote overall health and wellbeing. All Transformation™ formulas are carefully prepared to assure maximum quality and nutri-tional effectiveness.

When food intolerances have gone undetected for a time, inflammatory conditions may persist in the GI tract and/or systemically throughout the body. Synergistic formulas that

may be considered are PureZyme (or TPP Protease) and Plantadophilus (or TPP Probiotic).

Recommended UsageKidz Digest Powder: 1/8 tsp (1/2 scoop) mixed in a spoon of tepid water or placed on the tongue or as directed by your healthcare professional. Do not add to a bottle of baby formula or mix in baby food because it will break down the food in the container making it less palatable.

Kidz Digest Chewable: Two (2) tablets with every meal or as directed by your health care professional.

References

AUTISM & GMO’S

Hertz-Picciotto I, Delwiche L. “The Rise in Autism and the Role of Age at Diagnosis.” Epidemiology. 2009 Jan;20(1):84-90.

www.huffingtonpost.com/birgitta-lauren/how-safe-are-infant- formu_b_171512.html

www.Ucsdnutitionlink.org/articles/archives/fastfood.pdf

ENZYME EFFICACY & STABILITY

Borowitz D, et al. “Liprotamase Long-term Safety and Support of Nutritional Status in Pancreatic-insufficient Cystic Fibrosis.” Jour-nal of Pediatric Gastroenterology & Nutrition. 2012 Feb;54(2):248-257.

Graham D. “Ins and outs of pancreatic enzymes and adjuvant therapies.” Ped Gast and Nut. 1984. 3(suppl 1): S120.

Mamadou M, Marr S, Paydon K, Medhektar R. “Stability and Activity of Supplemental Digestive Enzymes in Simulated Gastric Fluid” presented at the Scripps Conference in San Diego, January 7-9, 2005.

Medhekar, R. “The First Quantitative Evidence Proving the Ef-ficacy Of Supplemental Enzymes.” National Enzyme Company. 2004.

PROTEASE

Buford TW, Cooke MB, Redd LL, Hudson GM, Shelmadine BD, Willoughby DS. “Protease supplementation improves muscle function after eccentric exercise.” Med Sci Sports Exerc. 2009 Oct;41(10):1908.

DDP-IV

Hausch F, Shan L, Santiago NA, Gray GM, Khosla C. “Intestinal digestive resistance of immunodominant gliadin peptides.” Am J Physiol Gastrointest Liver Physiol. 2002 Oct;283(4):G996-G1003.

Koch S, Anthonsen D, Skovbjerg H, Sjöström H. “On the role of

dipeptidyl peptidase IV in the digestion of an immunodominant epitope in celiac disease.” Adv Exp Med Biol. 2003;524:181-7.

Stepniak D, Spaenij-Dekking L, Mitea C, Moester M, de Ru A, Baak-Pablo R, van Veelen P, Edens L, Koning F. “Highly efficient gluten degradation with a newly identified prolyl endoprotease: implications for celiac disease.” Am J Physiol Gastrointest Liver Physiol. 2006 Oct;291(4):G621-9.

BROMELAIN

Choi HS, Sa YS. “Fibrinolytic and antithrombotic protease from Spirodela polyrhiza.” Biosci Biotechnol Biochem. 2001 Apr;65(4):781-6.

Felton GE. “Fibrinolytic and antithrombotic action of bromelain may eliminate thrombosis in heart patients.” Med Hypotheses. 1980 Nov;6(11):1123-33.

Kumakura S, Yamashita M, Tsurufuji S. “Effect of bromelain on kaolin-induced inflammation in rats.” Eur J Pharmacol. 1988 Jun 10;150(3):295-301.

Maurer HR. “Bromelain: biochemistry, pharmacology and medical use.” Cell Mol Life Sci. 2001 Aug;58(9):1234-45.

Taussig SJ, Batkin S. “Bromelain, the enzyme complex of pineap-ple (Ananas comosus) and its clinical application. An update.” J Ethnopharmacol. 1988 Feb-Mar;22(2):191-203.

AN AO

Ganiats TG, Norcross WA, Halverson AL, Burford PA, Palinkas LA. “Does Beano prevent gas? A double-blind crossover study of oral alpha-galactosidase to treat dietary oligosaccharide intoler-ance.” J Fam Prac. 1994; 39(5):441-5.

Graham H, Fadel JG, Newman CW, Newman RK. “Effect of pel-leting and B-glucanase supplementation on the ileal and fecal digestibility of a barley-based diet in the pig.” J Anim Sci. 1989; 67:1293-1298

Mroz Z, Jongbloed AW, Kemme PA. “Apparent digestibility and retention of nutrients bound to phytate complexes as influenced

Science Brief: Enzyme Nutrition for Kids Lisa Hudson, RDN • page 8

by microbial phytase and feeding regimen in pigs.” J Anim Sci. 1994 Jan;72(1):126-32.

Murai A, Kobayashi T, Okada T, Okumura J. “Improvement of growth and nutritive value in chicks with non-genetically modified phytase product from Aspergillus niger.” Br Poult Sci. 2002; 43(5 Suppl):687-95.

Nakae Y, Onouchi H, Kagaya M, Kondo T. “Effects of aging and gastric lipolysis on gastric emptying of lipid in liquid meal.” J Gas-troenterol. 1999; 34:445-449.

PROBIOTICS

Brown, D. “Bifidobacterium infantis effectively treats IBS symp-toms in adults.” Original Internist. Sep 2005.

Haros M, Bielecka M, Honke, J, Sanz Y. “Myo-inositol hexakispho-sphate degradation by Bifidobacterium infantis ATCC 15697.”Int J

Food Microbiol 117(1):76-84 (2007)

Swiss Institute of Bioinformatics. “HAMAP: Bifidobacterium longum subsp. infantis (strain ATCC 15697 / DSM 20088 / NCTC 11817 / S12) complete proteome.” http://expasy.org/sprot/hamap/BIFLS.html

DIETARY INGREDIENTS

Physicians’ Desk Reference for Herbal Medicines, 3rd edition. Montvale, NJ: Thompson PDR; 2004.

The Complete German Commission E Monographs, Therapeutic Guide to Herbal Medicines, 1st ed. Integrative Medicine Commu-nications, 1998.

http://naturaldatabase.therapeuticresearch.com

Copyright © Transformation Enzyme Corporation

Questions? 1-800-777-1474email moreinfo@tecenzymes.comwww.transformationenzymes.com

*These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure, or prevent any disease.

About the AuthorLisa Hudson, RDN, is a graduate of the University of Texas-Austin and the author of ‘The Ripple Effect of Toxicity: And What YOU Can Do About It.’