SCHEME OF TEACHING AND EXAMINATION B.E. V …SCHEME OF TEACHING AND EXAMINATION B.E. V SEMESTER (2014-15) Sl. No. Subject Code Subject Credits Hours/Week Examination Marks Lecture

SCHEME OF TEACHING AND EXAMINATION B.E. V SEMESTER (2014-15)

Sl. No.

Subject Code

Subject Credits Hours/Week Examination Marks

Lecture Tutorial Practical CIE SEE Total

1 UBT501C Enzyme kinetics(PC) 3 3 0 0 50 50 100

2 UBT503C Bioinformatics(PC) 4 3 2 0 50 50 100

3 UBT504C Genetic Engineering & Applications(PC)

4 4 0 0 50 50 100

4 UBT516C Bioprocess & Reaction Engg. (PC)

4 3 2 0 50 50 100

5 UBT521E Elective –1 3 3 0 0 50 50 100

6 UBT517C Plant and animal cell

culture techniques(PC) 3 3

0 0

50 50 100

7 UBT508L Bioinformatics Lab 1.5 0 0 3 50 50 100

8 UBT509L Genetic Engineering Lab 1.5 0 0 3 50 50 100

Total 24 19 4 6 400 400 800

Elective -1 UBT521E: Environmental BT UBT523E: Operating system and DBMS

UBT522E: Biomedical Instrumentation UBT525E: Stem cell technology

SCHEME OF TEACHING AND EXAMINATION B.E. VI SEMESTER (2014-15)

Sl.

No.

Subject

Code

Subject Credits Hours/Week Examination

Marks

Lecture Tutorial Practical CIE SEE Total

1 UBT612C Bio-transformation & Enzyme Technology (PC)

4 4 0 0 50 50 100

2 UBT604C Bioprocess Equipment Design & Drawing (PC)

4 3 2 0 50 50 100

3 UBT602C Bioprocess control and automation

4 4 0 0 50 50 100

4

UBT605H Bioethics and biosafety

3 3 0 0 50 50 100

5 UBT62XE Elective-2 3 3 0 0 50 50 100

6 UBT62XE Elective – 3 3 3 0 0 50 50 100

7 UBT608L Bio-kinetics & Enzyme Technology Lab

1.5 0 0 3 50 50 100

8 UBT609L Bioprocess control and automation Lab

1.5 0 0 3 50 50 100

9 UBT611L Biostatistics lab 2 0 0 4 50 50 100

Total 26 20 2 10 450 450 900

Elective- 2 & 3

UBT621E Microbial BT UBT622E Genomics & Proteomics

UBT623E Plant BT UBT624E Animal BT UBT625E Biofuels technology UBT 626E Pearl programming UBT 627E Tissue engineering UBT 628E Transport phenomena

UBT501C: ENZYME KINETICS 3 Credits (3-0-0)

Prerequisites : Biochemistry, Structural biology

Course Objectives:

To design the experiment and analyse the data.

To solve the kinetics of enzyme catalysed reactions.

To understand the properties of the enzymes.

To understand the Isolation and purification techniques of enzymes.

To understand the how enzymes work.

UNIT- 1

INTRODUCTION TO ENZYMES: 10 Hours Definition, Classification of Enzymes; Chemical nature and properties of Enzymes, Factors affecting

Enzyme activities, Active site, allosteric site, coenzymes and co factors. Isoenzymes,

Units of Enzyme activity. .

UNIT- 2

ENZYMATIC TECHNIQUES: 10 Hours

Strategies of purification of enzymes, criteria of purity, molecular weight determination and characterization of

enzymes. Enzyme assay, continuous and discontinuous assay (with 2 examples – LDH & CK)

UNIT- 3

ENZYME SPECIFICITY: 10 Hours

Types of Enzyme specificities. Enzyme substrate reactions. Mechanism of enzyme action- lock and key model,

Induced fit hypothesis, substrate strain theory (with lysozyme as a typical example). Enzyme kinetics

Derivations of Km value (Michaelis-Menton constant), Lineweaver-Burk plot., velocity, rate of reaction, order,

free energy of activation, zero order, and first order. Mechanism of Enzyme catalysis - Acid-Base catalysis,

covalent catalysis and -entropy effect. and Enzyme inhibition and kinetics

UNIT- 4

MULTI-SUBSTRATE REACTIONS: 10 Hours

Regulation of enzyme activity, Allosteric regulation, feed back regulation, cascade system (genetic regulation),

Introduction to enzyme catalyzed reaction Ping-pong mechanism, Sequential mechanism (ordered and random),

Enzyme models - Host guest complexation chemistry.

Total: 40Hours

Course outcomes:

1. Ability to Classify and characterize the enzymes

2. Ability to evaluate the advantages of enzymes over chemicals

3. Ability to understand the enzyme catalysed biotransformation

4. Ability to prepare the immobilization and analyze its kinetics.

5. Ability to understand the bioconversion processes using enzymes

6. Design and construct novel and artificial enzymes.

7. Apply the knowledge of diagnosis of various diseases

8. Ability to develop skills to modify materials for food and other industries

REFERENCE BOOKS:

1. Enzymes Biochemistry , Biotechnology, Clinical Chemistry by Trevor Palmer, Horwood Publishing Ltd,

First East-West Press Edition 2004

2. David L. Nelson and Michael Cox, “Lehninger Principles of Biochemistry” –6th

Edition,

3. An Introduction to Biotransformation in Organic Chemistry by James R Hanson. W H Freeman.

4. Contemporary Enzyme Kinetics and Mechanism by Daniel L. Purich, Melvin I. Simon, John N. Abelson,

2000.

5. Biotransformations: K. Faber (1995), Springer- Verlag.

6. Bailey and Ollis, Biochemical Engineering Fundamentals, Mcgraw Hill (2nd Ed.), 1986.

7. Enzyme Kinetics by Plowman, McGraw Hill, 2010

8 .Mechanisms in Protein Chemistry by Jack Kite, Garland Publishers, 1995. Bio-organic chemistry of Enzyme

catalysis by M L Bender, R J Bergerson and M Komiyana, Wiley 1984

QUESTION PAPER PATTERN OF CIE (Continuous Internal Evaluation):

1. CIE comprises of 3 tests, each of 30 marks and I hr duration,totaling to 90 marks and later is scaled down to

45 marks

2. Each CIE Will be Covering Complete unit

3. Any two full questions to be answered out of three questions and each question carries fifteen marks

4. Assignment/ Quiz/objective tests carries five marks

QUESTION PAPER RATTERN OF SEE

1. Total of Eight Question with Two from each unit to be set uniformly covering the entire syllabus.

2. Each question should not have more than four sub questions.

3. Each full question carries 20 marks

4. Any five full questions are to be answered choosing at least one from each unit.

UBT503C: BIOINFORMATICS

4 Credits (4-0-0)

Prerequisites : Biochemistry Molecular biology,

Bio-statistics, Cell biology & Genetics, Computer Applications

Course Objectives:

To study Databases and its types and tools.

To understand the sequence alignment and database searches

To know about phylogenetic analysis.

To study Restriction mapping and Sequencing methods.

To know about applications of insilico modeling in modern biology.

To study insilico drug design.

UNIT- 1

DATABASES&TOOLS: 13 Hours

Introduction to bioinformatics, meaning of databases, types of databases. The nucleotide and protein sequence

Databases: GenBank, DDBJ and EMBL. Primary and Secondary Databases; File format, contents, search of

databases- Gene bank flat file. Protein Data Bank (PDB) flat file; FASTA Format, PIR Format; Structure file

formats, PDBSUM, PDB Lite, MMDB, SCOP, Specialized databases: NCBI, PubMed, OMIM, Medical

databases and KEGG.

SEQUENCE ALIGNMENT AND DATABASE SEARCHES

Introduction-The evolutionary basis of sequence alignment, the Modular Nature of proteins, Database similarity

searching, FASTA, BLAST. PSI-BLAST & PHI-BLAST. Pair wise and multiple alignments of sequences,

progressive alignment methods, CLUSTAL-W, Motifs and Patterns, PROSITE, 3DPSSM, DOT PLOT,

Optimal Alignment Methods- dynamic programming, e-value, gap penalties, insertions and deletions in

sequences.

UNIT- 2

PHYLOGENETIC ANALYSIS: 13Hours

Introduction, concepts of trees, phylogenetic trees and multiple alignments. Distance matrix method (MD),

character based methods, methods of evaluating phylogenesis, summary of the phylogenetic methods. Steps in

constructing alignments and phylogenesis. Phylogenetic softwares (CLUSTAL W, PHYLIP etc),

PREDICTIVE METHODS

Predictive Methods using Nucleotide sequences: Framework, Masking repetitive DNA, Database searches,

Codon Bias Detection, Detecting Functional Sites in the DNA (promoters, transcription factor binding

sites, translation initiation sites), Integrated Gene Parsing, finding RNA Genes, Web based tools

(GENSCAN, GRAIL, GENEFINDER). Predictive Methods using Protein sequences: Protein Identity based on

composition, Physical properties Based on sequence, secondary structure and folding classes, specialized

structures or features, tertiary structure. Related web based software (JPRED, PROSEC, NNPREDICT and

SOPMA)

UNIT- 3

PLASMID MAPPINGAND PRIMER DESIGN: 13Hours

Restriction mapping, Utilities, DNA strider, Mac Vector and OMIGA, gene construction KIT, Web based tools

(MAP, REBASE); Primer design – need for tools, Primer design programs and software (PRIME3).

GENOME BIOINFORMATICS:

Sequencing methods, Bioinformatics tools and automation in Genome Sequencing, analysis of raw genome

sequence data, Utility of EST database in sequencing, Bioinformatics in detection of Polymorphisms, SNPs and

their relevance, Bioinformatics tools in microarray data analysis. Tools for comparative genomics: BLAST2,

AVID, Vista, MUMmer, COG, VOG. Qualitative discussions on Machine Learning Tools (Artificial

Intelligence, Genetic algorithm and neural networks).

UNIT- 4

MOLECULAR VISUALIZATION: 13Hours

Scope and applications of insilico modeling in modern biology. Comparative modeling, constructing an initial

model, refining the model, manipulating the model; molecular superposition and structural alignment, concept

of energy minimization. Graphical representation of molecular structures: small molecules (low molecular

weight – peptides, nucleotides, disaccharides, simple drugs molecules) and macromolecules (high molecular

weight molecules- proteins, DNA, RNA, membrane proteins). Usages of visualization software available in

public domain like VMD, Rasmol, Spdb Viewer, and Cn3D.

INSILICO DRUG DESIGN:

Molecular modeling in drug discovery, deriving bioactive conformations, molecular docking, quantitative

structure-activity relationship (QSAR), deriving the Pharmacophoric Pattern, Receptor Mapping, Estimating

Biological Activities, Ligand - Receptor Interactions: Docking softwares (AUTODOCK, HEX) Calculation of

Molecular Properties, Energy Calculations (no derivation).

Total: 52 Hours

Course Outcomes:

Able understand the Databases and its types and tools.

Able to understand the sequence alignment and database searches

Able to know about phylogenetic analysis.

Able to understand Restriction mapping and Sequencing methods.

Able to know applications of insilico modeling in modern biology.

Able to study insilico drug design.

REFERENCE BOOKS

1. Bioinformatics – Andreas D Baxevanis. Wiley Interscience, 1998.

2. Bioinformatics –David W Mount, cold spring harbor, 2005.

3. Introduction to Bioinformatics – Arthur Lesk, Oxford, 2006.

4. Bioinformatics – Stuart M Brown, NYU Medical Center, NY USA. 2000.

5. Fundamental Concepts of Bioinformatics – D E Krane & M L Raymer, Pearson, 2006.

6. Computational methods for macromolecular sequence analysis – R F Doolittle. academic Press,

1996.

7. Computational methods in Molecular Biology – S.L.Salzberg, D B Searls, S. Kasif, Elsevier, 1998.

8. Bioinformatics, Methods And Applications– Genomics, Proteomics And Drug discovery – s c

rastogi, n mendiratta & p rastogi, phi, 2006.

9. The molecular modeling perspective in drug design – N Claude Cohen, 1996, Academic Press

10. Analytical Tools for DNA, Genes & Genomes: – Arseni Markoff, New Age, 2007.

11. Introduction to bioinformatics – anna tramontano (2007) taylor & francis.

12. Bioinformatics – des higgins & willie taylor (2005) oxford.


1. CIE comprises of 3 tests, each of 30 marks and I hr duration,totaling to 90 marks and later is scaled

down to 45 marks




QUESTION PAPER PATTERN OF SEE




4. Any Five Full questions are to be answered choosing at least one from each unit.

UBT504C: GENETIC ENGINEERING & APPLICATIONS

4 Credits (4-0-0)

Prerequisites : Molecular Biology

Course Objectives:

Understand the basics of genetic engineering

Learn different methodologies in genetic engineering

Design and fabricate a desired construct

Ability to design and implement protocols in genetic engineering

Develop project plans pertaining to recombinant DNA technology

Develop project plans pertaining to recombinant DNA technology

UNIT- 1

INTRODUCTION 13Hours

Role of genes within cells, genetic elements that control gene expression, Tools of genetic engineering- vectors

in recombinant DNA technology, biology and salient features of vectors, Types of vectors - plasmids, cosmids,

phages and viruses.

ENZYMES IN GENETIC ENGINEERING

Introduction- Restriction Endonucleases-classification, mode of action, applications. Enzymes used in nucleic

acid modification – Alkaline phosphatase, Polynucleotide phosphorylase , DNase, polynucleotide Kinase,

Ligases, RNase, terminal deoxy nucleotidyl transferase,S1 nuclease, T4 DNA polymerase, Poly A polymerase.

UNIT- 2

NUCLEIC ACID HYBRIDIZATION AND AMPLIFICATION 13Hours

Methods of nucleic acid detection, Fluorescent In situ hybridization(FISH), colony hybridization, polymerase

chain reaction (PCR), its types and applications, methods of nucleic acid hybridization- probe and target

sequences, Southern, Western and Northern hybridization techniques.

CONSTRUCTION OF DNA LIBRARIES

Isolation and purification of nucleic acids, quantification, Isolation of plasmids, Construction of cDNA,

genomic and cDNA libraries, screening and preservation of libraries.

UNIT- 3

GENE TRANSFER TECHNIQUES 13Hours

Gene transfer techniques in plants, animals and microbes –Transformation, microinjection, electroporation,

microprojectile system, and liposome mediated transfer, embryonic stem cell method. Agrobacterium-mediated

gene transfer in plants – Ti & Ri Plasmid: structure and functions, Ti based vectors- Binary vectors and

Cointegrate vectors.

TRANSGENIC SCIENCE AND GENETIC IMPROVEMENT

Transgenic science in plant improvement, biopharming – plants as bioreactors, transgenic crops for increased

yield, Antisense RNA technology (Flavr savr tomatoes). Application of plant transformation for productivity

and performance – Herbicide resistance - glyphosate. insect resistance - Bt genes ( Bacillus thuringiensis and

its mode of action), Cry proteins – mechanism of action. Transgenic for disease resistance- coat protein

mediated, PR proteins. Abiotic stress – drought and salinity.

UNIT- 4

GENE THERAPY 13 Hours

Introduction, Methods of Gene therapy-gene targeting, gene augmentation, assisted killing, prodrug therapy and

gene silencing. Gene therapy in the treatment of cancer, SCID, muscular dystrophy, Use of genetically modified

and humanized antibodies against cell surface antigens to prevent the spread of breast cancers and prevent organ

graft rejection. Use of thrombolytic agents in blood clotting. Challenges in gene therapy.

OTHER APPLICATIONS

Microbial biotechnology - Genetic manipulation, engineering microbes for the production of Insulin, growth

hormones, monoclonal antibodies.

Total: 52 Hours

Course outcomes:

Understand analyze and apply the techniques in various fields of biotechnology.

Ability to design and implement protocol in genetic engineering.

REFERENCE BOOKS 1. Introduction to Genetic Engineering by Nicholl. Cambridge third Edition, 2008.

2. Molecular Biotechnology by Glick

3. Recombinant DNA Technology by T.A.Brown

4. Principles of gene manipulation - An introduction to genetic engineering, Old R.W.,

5. Primrose S.B., Blackwell Scientific Publications, 1993.

6. From Genetics to Gene Therapy – the molecular pathology of human disease by

7. David S Latchman, BIOS scientific publishers, 1994.

8. Genes VIII by Benjamin Lewis. Oxford University & Cell Press, 2007.

9. DNA Science by David A Micklos, Greg A Freyer and David A Crotty, I K International, 2003

10. Molecular Biotechnology: Principles and Practices by Channarayappa, 2006, University Press.

11. Genetic Engineering Vol. 1-4 (Williamson Edition)

12. Recombinant DNA by Watson et al., 2010.

13. Vectors by Rodriguer and Denhardt, 1987.

14. Current protocols in molecular biology, Greena Publishing Associates, NY, 1988.



down to 45 marks









UBT516C: BIOPROCESS & REACTION ENGINEERING

4 Credits (4-0-0)

Prerequisites : Unit Operations

Course Objectives:

Explain the different types reactions

Distinguish between reactions and rate of reactions

Derive the design equations

Distinguish between biochemical reactions

Derive the equations for RTD for different types of reactors

Calculate the Kinetics of Microbial Growth

Explain the Growth and Product Formation

Calculate the Kinetics of substrate uptake in cell culture

Analyze the Kinetics of substrate uptake in cell culture

Estimate energetic analysis of microbial growth and product formation

Calculate the Mass Transfer in heterogeneous biochemical reaction systems.

UNIT- 1

Introduction: 13 Hours

Law of mass action and rate equation, definitions and examples of elementary and non elementary reactions,

theories of reaction rate and temperature dependency, analysis of experimental reactor data: evaluation of rate

equation, integral and deferential analysis for constant volume system. Conceptual numerical.

Biochemical Equilibria:

Equilibrium in chemically reactive systems (single and multiple reactions), evaluation of reaction equilibrium

constant, effect of temperature on equilibrium- derivation of G vs. T relation, application of above concepts to

biochemical systems. Conceptual numerical.

UNIT- 2

Bioreactors: 13 Hours

Design equations for homogeneous systems: batch, stirred tank and tubular flow reactor, size comparison of

reactor systems, combination reactor systems. Optimization of output and yield problems, qualitative design for

consecutive, parallel and mixed reactions. Factors affecting choice of reactors. Conceptual numerical.

Non-Ideal Bioreactors:

Non-ideal reactors, residence time distribution studies, pulse and step input response of reactors, RTD’s for

CSTR and PFR, calculations of conversions for first order reactions, tanks in series and dispersion models.

Conceptual numerical.

UNIT- 3

Kinetics of Microbial Growth and Product Formation: 13 Hours

Yields in cell culture, Cell growth kinetics: Batch growth, Balanced Growth: Monod equation, Effects of

substrate concentration, Production kinetics in cell culture: Directly/ indirectly coupled with energy metabolism,

Not coupled with energy metabolism.

Kinetics of substrate uptake in cell culture: in the absence/ in association of product formation. Effect of culture

conditions on cell Kinetics. Determining cell kinetic parameters from batch data, rates of growth, product

formation and substrate uptake, cell doubling time μmax, and Ks with with conceptual numerical. Effect of

maintenance on yields. Observed yields, Biomass/ product yield from substrate, product yield from biomass,

Leudeking-Piret models. Kinetics of cell death with conceptual numerical.

UNIT- 4

Thermodynamics of microbial growth: 13 Hours

Energetic analysis of microbial growth and product formation, Heat of reaction with oxygen as/ not as principal

electron acceptor. Energy balance equation for cell culture.

Transport Phenomena In Biochemical Reactors:

Mass Transfer in heterogeneous biochemical reaction systems. Oxygen transfer in submerged fermentation

processes; oxygen uptake rates and determination of oxygen transfer coefficients (KLa); role of aeration and

agitation in oxygen transfer. y

Total: 52Hours

Course outcomes:

Define the types of reactions, evaluations of rate of reactions

Apply the applications of biochemical reactions, equilibrium constants

Derive the design equations for different types of bioreactors

Calculaate the RTD for different types of reactors

Evaluate the Kinetics of Microbial Growth and Product Formation

Explain the Kinetics of substrate uptake in cell culture

Find Energetic analysis of microbial growth and product formation,

Apply the Mass Transfer in heterogeneous biochemical reaction systems

TEXT BOOKS:

1. Chemical Reaction Engineering- Levenspiel O- John Wiley, 3rd

Edition, 2006.

2. Elements of Chemical Reaction Engineering- Fogler, H.S. Prentice Hall, 1986.

3. Bioprocess Engineering- Shuler and Kargi Prentice Hall, 1992.

4. Enzyme Kinetics and Mechanism- Paul F Cook & WW Cleland – Garland Science

REFERENCE BOOKS:

1. Bioprocess Engineering- Aiba, Humprey & Millis, Academic Press.

2. Biochemical Engineering – James Lee, 1992.

3. Biochemical Engineering Fundamentals- Bailey and Olli’s, McGraw Hill (2nd

Ed.) 1986

4. Bioprocess Engineering Principles – Pauline M. Doran, 1995. London.

5. Principles of Biochemistry – Leninger A.L.., II Edition, 1993.

6. Enzyme Kinetics- Plowman, McGraw Hill, 1972.

7. Chemical Engineering Kinetics- Smith J.M.., McGraw Hill, 3rd

Edition, New Delhi, 1981.

8. Bioprocess Engineering- Wolf R. Vieth- Kinetics, Mass Transport, Reactors and Gene

Expression. A Wiley – Interscience Publication, 1992

9. Chemical Reactor Analysis and Design- Ferment G. F. and Bischoff K.B. John Wiley. 1979

10. Biocatalyst Membrane Reactor- Drioli, & Francis, 2005.



down to 45 marks




QUESTION PAPER PATTERN OF SEE:

1. Total of Eight Question with two from each unit to be set uniformly covering the entire

Syllabus.

2. Each Question should not have more than four sub questions.

3. Any Five questions are to be answered choosing at least one from each unit.

UBT517C: PLANT AND ANIMAL CELL CULTURE TECHNIQUES

3 Credits (3-0-0)

Prerequisites : Genetic engineering.

Course Objectives:

To know the fundamentals of plant and animal cell culturing.

To design and study the requirements of plant and animal cell culture labs.

To understand the behavior of animal cells In Vitro conditions..

To study various cell viability and toxicity assays

To know the applications of plant and animal cells in production of therapeutics.

To get detailed knowledge about assisted reproductive technology.

UNIT- 1

Plant cell culture 12 Hours

History and Introduction, requirements, lab organisation, media constituents, choice of media sterilization of

media, explant selection, sterilisation and preparation for inoculation, role of growth hormones in cell culture.

Types of culture Cellular totipotency, cytodifferentition, organogenic differentiation, embryogenesis.

Culture techniques and applications

Seed culture, embryo culture, synthetic seeds, callus culture,(types) organ culture, cell culture, protoplast

culture , somatic hybridization, haploid production ,micropropagation: somaclonal variation auxillary bud

proliferation, meristem, shoot tip and bud culture. Regeneration of plantlets-shooting, rooting and hardening.

UNIT- 2

Animal cell culture 10 Hours

History and development of mammalian cell culture. lab organization, equipment and materials (culture vessels,

CO2 incubator, inverted microscope, cell counters).Introduction to balanced salt solutions. Cell culture media -

components of the medium, physical, chemical and metabolic functions of media. Role of serum and

supplements, serum-free media, features and specifications of MEM, DMEM, RPMI and Ham’s medium Role

of antibiotics in media.

Techniques:

Measurement of cell number - hemocytometer, coulter counter. Measurement of cell viability and cytotoxicity.

Dye exclusion and inclusion tests, colonigenic assay, and MTT, PDT.

UNIT- 3

Cell lines 10Hours

Primary culture – Mechanical and enzymatic mode of desegregation, establishment of primary culture.

Subculture - passage number, split ratio, seeding efficiency, criteria for subculture. Cell lines -definite and

continuous cell lines, characterization, maintainance and preservation of cell lines (cryopreservation). Cell line

contaminations, detection and control, cell transformation – normal v/s. Transformed cells, growth

characteristics of transformed cells.

UNIT- 4

Manipulation of Reproduction

10 Hours

Conventional methods of animal improvement, predominantly selective breeding and crossbreeding. Artificial

insemination.Invitro fertilization(IVF) and embryo transfer technique(ETT).Embryo splitting,Stem cells -

embryonic and adult stem cells, Nuclear transplantation(Dolly)

Transgenics and applications: Cloning of animals - Transgenic animals (cow, sheep, bird, fish) - retroviral,

microinjection, and engineered embryonic stem cell method of transgenesis. Methods of transfection of cells.

Application of animal cell culture -Large scale production of recombinant gene products cell culture based

vaccines. Diagnosis of genetic diseases through cell culture

Total: 42 Hours

Course Outcomes:

Able to apply the knowledge in identify the structure and growth pattern of plant and animal cells.

Able to analyze the cell line contamination.

Ability to understand the concept of ART in the field of livestock.

Able to apply the knowledge in identifying various genetic diseases.

REFERENCE BOOKS

1. Culture of Animal Cells – (3rd Edn) R Ian Freshney. Wiley-Liss 2010

2. Animal Cell Biotechnology–Spier, RE and Griffith, JB Academic Press, London 2010

3. Animal Biotechnology – Murray Moo-Young Pergamon Press, Oxford ,2010 ,

4. Animal Cell Technology: Principles and practices– Butter, M Oxford press, 2010

5. An Introduction To Molecular Biotechnology – Michael Wink, Wiley, 2008

6. Plant Cell Culture: A Practical Approach – R.A. Dixon & Gonzales, IRL Press.

7. Plant Tissue Culture: Applications and Limitations – S .S. Bhojwani Elsevier, Amsterdam. 1990

8. Methods in Cell Biology, Vol. 57, Animal Cell Culture Methods – Ed. JP Mather and D

Bames.Academic Press

9. Fish and Fisheries India – VG Jhingram

10.Living resources for Biotechnology, Animal cells – A. Doyle, R. Hay and B.E. Kirsop (1990),

Cambridge University Press, Cambridge.

11. Animal Cell Culture Practical Approach, – Ed. John RW. Masters, Oxford press 2010

12. Animal Cell Culture Techniques – Ed Martin Clynes, Springer 2010

13. Cell Culture Lab Fax – Eds. M Butler & M Dawson, Bios Scientific Publications Ltd. Oxford 2010

14. Elements of Biotechnology – PK Gupta – Rastogi and Co. Meerut 1996.

15. Biotechnology in Agriculture – MS Swamynathan – McMillian India Ltd. 2010

16. Introduction to plant Biotechnology by H.S. Chawla, Second edition, Oxford & IBH Publishers,

2005



down to 45 marks.

2. Each CIE will be covering one complete unit


4. Assignment/quiz/ objective tests carries five mark






UBT521E: ENVIRONMENTAL BT

3 Credits (3-0-0)

Prerequisites : Microbiology, Environmental studies

Course Objectives:

To understand the importance of environment BT.

To understand the process of Bioaccumulation.

To understand the Waste treatment methods.

To understand the concept of bioleaching.

To understand types of Soil microbiota and their role.

To understand the application of BT in ecosystem conservation

UNIT- 1

MICROORGANISMS 10 Hours

Overview of microorganisms, issues and scope of Environmental BT. Characteristics of soil, microbial flora of

soil, interactions among soil microorganisms, biogeochemical role of soil microorganisms.

BIOACCUMULATION OF TOXICANTS

Characteristics of Xenobiotics, Relationship of Bioaccumulation with Chemical Structure, Ecophysiology of

Bioaccumulation, Process of toxicants uptake, Factors affecting bioaccumulation, measurement of

bioaccumulation.

UNIT- 2

BIOLOGICAL TREATMENT OF WASTE WATER 12Hours

Waste water characteristics, , BOD, COD, Primary & Secondary treatment, Microbial removal of phosphorous

and Nitrogen, Nutrient removal by Biomass production

Wastewater treatment of food processing industries like sugar factories, vegetable oil industries, potato

processing industries, dairy industries, beverages industries, and distilleries.

SOLID WASTE MANAGEMENT Basic aspects, general composition of urban solid wastes, aerobic treatment, anaerobic treatment, biogas

generation; Solid waste management through Biotechnological processes involving Hazardous wastes,

Biomedical wastes

UNIT- 3

BIOLEACHING & BIOMINING 10 Hours

Microbes in Bioleaching- types, methods of bioleaching, Microbial recovery of metal, phosphate, petroleum.

BIOFUELS Defination, Renewable and non renewable resources. Adavantages & dis advantages of biofuels.

.Bio-fuel feed stocks –sugar, starch, cellulose, lipid. Types of Biofuel – first, second and third generation

.Technologies for bio-fuel production- transesterification, gassification,. Issues of biofuel production & its use.

Microbial fuel cells.

UNIT- 4

BIOREMEDIATION: 10 Hours

Major contaminants of air, water and soil, Biomonitors of environment (Bioindicators), Bioremediation using

microbes, Phytoremediation, Biofilms.

BIOTECHNOLOGY IN BIODIVERSITY CONSERVATION

Value of biodiversity, threats to biodiversity, Biosphere reserves and Ecosystem Conservation, Approaches to

Bioresource conservation programme, Biotechnological processes for bioresource assessment, BT in ex situ

conservation of Biodiversity, BT and its role in utilization of Biodiversity, International initiatives for

biodiversity management.

Total: 42 Hours

Course outcomes:

Ability to Understand issues and scope of Environmental BT

Ability to understand Characteristics of Xenobiotics and Bioaccumulation process.

Ability to understand Waste water treatment methods and Solid waste management

through Biotechnological processes

Ability to understand Methods of bioleaching for metal recovery

Ability to understand Bioremediation process for removal of contaminants.

Ability to apply biotechnological approaches for Biosphere reserves and Ecosystem

Conservation

Student will understand the Value of biodiversity and threats to biodiversity

Ability to understand biogeochemical role of soil microorganisms

REFERENCE BOOKS

1. Environmental Biotechnology by Foster C.F., John ware D.A., Ellis Horwood Lmited,1987.

2. Bioprocess Technology- fundamentals and applications, S O Enfors & L Hagstrom (1992), RIT,.

3. Comprehensive Biotechnology Vol. 1- 4 : M.Y. Young (Eds.), Pergamon Press.

4. Industrial Microbiology : L.E. Casida, Willey Eastern Ltd., 1989.

5. Industrial Microbiology : Prescott & Dunn, CBS Publishers, 1987.

6. Biotechnology, Economic & Social Aspects : E.J. Dasilva, C Ratledge & A Sasson, Cambridge Univ. Press,

Cambridge.

7. Environmental Biotechnology by Pradipta Kumar Mahopatra.



down to 45 marks





1. Total eight Question with Two from each unit to be set uniformly covering the entire syllabus.


3. Any Five Full questions to be answered choosing at least one from each unit.

UBT522E: BIOMEDICAL INSTRUMENTATION

3 Credits (3-0-0)

Prerequisites : Microbiology, instrumentation

Course Objectives:

To know the basic concepts of biomedical signals

To know about ECG and EEG

To know about patient monitoring system

To know about recording systems

UNIT- 1

INTRODUCTION 1 10 Hours

Sources of Biomedical signals, Basic medical instrumentation system, Performance requirements of medical

instrumentation systems, PC based medical instruments, General constraints in design of medical

instrumentation systems. 4 Hours UNIT 2. BIOELECTRIC SIGNALS AND ELECTRODE

Origin of bioelectric signals, Recording electrodes, - Electrode-tissue interface, metal electrolyte interface,

electrolyte -skin interface, Polarization, Skin contact impedance, Silver – silver chloride electrodes, Electrodes

for ECG, EEG, EMG, Electrical conductivity of electrode jellies and creams, Microelectrode. Patient Safety:

Electrode shock hazards, Leakage currents.

UNIT- 2

ECG & EEG 10 Hours

Electrical activity of heart, Genesis & characteristics of Electrocardiogram (ECG), Block diagram description of

an Electrocardiograph, ECG Lead Systems, Multichannel ECG machine Genesis of Electroencephalogram

(EEG), Block diagram description of an Electroencephalograph, 10-20 Electrode system, Computerized

analysis of EEG.

CARDIAC PACEMAKERS AND DEFIBRILLATORS

Need for Cardiac pacemaker, External pacemaker, Implantable pacemaker, Programmable pacemakers, DC

defibrillator, AC defibrillator and Implantable Defibrillator.

UNIT- 3

PATIENT MONITORING SYSTEM 10 Hours

Bedside monitors, Central Monitoring System, Measurement of Heart rate -Average heart rate meter,

Instantaneous heart rate meter, (Cardio tachometer), Measurement of Pulse Rate, Blood pressure measurement -

direct and indirect method, Rheographic method, Oscillometric method, Ultrasonic Doppler shift method,

Measurements of Respiration rate -Thermistor method, impedance puenmography, CO2 method, and Apnea

detector. Blood flow meters: Electromagnetic and its types, Ultrasonic, NMR, Laser Doppler. Blood gas

analyzers: Blood pH measurement, Measurement of Blood pCO2, pO2.

PHYSIOLOGICAL TRANSDUCERS

Introduction, classification, performance characteristics of transducers-static and dynamic transducers,

Displacement, position and motion transducers, Pressure transducer, Transducers for body temperature

measurement, Optical Fiber sensor and Biosensor

UNIT- 4

RECORDING SYSTEMS 10 Hours

Basic recoding system, general considerations for signal conditioners, preamplifiers-instrumentation amplifier,

isolation amplifier, ink jet recorder, potentiometric recorder, thermal array recorder and electrostatic recorder. 4

Hours UNIT 8. ANALYSIS a) Cardiac output measurement: Indicator dilution method, Dye dilution method,

Thermal dilution techniques, Measurement of Continuous cardiac output derived from the aortic pressure

waveform, Impedance technique. 4 Hours b) Pulmonary function analysis: Pulmonary function measurement,

Spirometry, Puemotachometer, Measurement of Volume, Nitrogen washout technique.

Total: 40 Hours

Course Outcomes:

Able to understand basic concepts of biomedical signals.

Able to know ECG and EEG.

Able to understand the patient monitoring system and recording systems

REFERENCE BOOKS

1. Hand book of Biomedical Instrumentation – R. S. Khandpur, 2nd

Edition, Tata McGraw-Hill Publishing

Company Limited, 2003.

2. Introduction to Biomedical Engineering by J Enderle, S Blanchard & J Bronzino, Elsevier, 2005.

3. Encyclopedia of Medical devices and Instrumentation – J G Webster – John Wiley 1999.

4. Principals of applied Biomedical instrumentation – John Wiley and sons

5. Introduction to Biomedical equipment technology – Joseph J Carr, John M Brown Prentice hall 4th

Edition.



down to 45 marks.



4. Assignment/quiz/ objective tests carries five marks





UBT523E OPERATING SYSTEMS AND DBMS

3 Credits (3-0-0)

UNIT- 1

INTRODUCTION 10 Hours

What is O.S, Von-Neumann architechture, Supercomputers, Mainframe systems, Desktop system,

Multiprocessor systems, Distributor systems, Clustered systems, Real time systems, Hand held systems, Future

migration, Computing environment, System components, services, System calls, System programs, system

structure, OS design and implementation, microkernels, virtual machines

PROCESS MANAGEMENT

Process concept, process state, process control block, process scheduling, snail diagrams, schedulers, creation

and removal of a process, interprocess communication, models for IPC, independent and cooperating processes,

threads, overview, multithreading, applications, critical selection problem, Semaphores, deadlocks and

starvation.

UNIT- 2

STORAGE MANAGEMENT 10 Hours

Memory management, dynamic loading and linking, overlays, logical vs physical space,

memory management unit, swapping, contigous allocation, fragmentation, paging, page table, segmentation,

virtual memory, demand paging, thrashing file system, interface-file concept, directory implementation.

LINUX AND WIN NT

Linux: Design principles, Kernel modules, process management, scheduling, memory

management systems, input and output, interprocess communication.

WinNT: Design principles, system components, environmental subsystems, file system,

networking and programming interface

UNIT- 3

DESIGN OF DBMS 10 Hours Introduction to DBMS, terminology, Systems Development Life Cycle, terms of reference, feasibility report,

data flow diagrams, addition of data sources, identification of individual processes, inputs and outputs, system

boundaries, Entity-Relationship modeling, examples, database creation using MS Access, designing tables

using Access, Data Integrity, Normalization, relationships between tables, comparing E-R design with

Normalization design, Inclusion of new requirements from feasibility report, documentation, amending primary

keys and database tables, Practical examples.

DATA DICTIONARY AND QUERY DESIGN

Data dictionary, criteria, compiling a list of field names, entry sequence for the table data, entering, sorting and

filtering of data in a table, introduction to queries, identifying field names, selection criteria and sort order in a

query, calculations in queries, modifying a query, creating a query using design view and wizard in MS Access

UNIT- 4

REPORTING, TESTING AND DOCUMENTATION 10 Hours

Introduction to reporting, dataflow diagram based reporting and table based reporting, form creation using

wizard, entering and searching records in a form, modifying forms and reports, Introduction to testing, types

(unit testing, system testing, integration testing, interface testing, performance testing and user testing), test

data, executing and error reporting, introduction to documentation, areas of documentation

SETTING UP THE DATA AND HOUSEKEEPING

Approaches to set up data (parallel, bigbang, phased and pilot implementation), working data, data entry

methods to the database (systems screen, external source), introduction to housekeeping, regular backups,

archiving old data, maintaining security in a database.

REFERENCE BOOKS

Mastering Database Design by Helen Holding, Macmillan publications, 1999.

Operating system concept by Silberschatz, peterhalvin and Greg Gague, VI edition, John Wiley, 2003.

Linux: the complete reference by Richard Peterson, McGraw Hill, 1998

Operating System – A concept based approach by D Dhamdene, Tata McGraw Hill, 2002.

The complete reference-By Coach and loney

A Beginners guide- By Abbey and corney Database System-Elmasri and Navathe



down to 45 marks.








UBT525E: STEM CELL TECHNOLOGY

Credits 3(3-0-0)

UNIT- I

STEM CELLS AND CELLULAR PEDIGREES 10hours Scope of stem cells – definition of stem cells – concepts of stem cells – differentiation , maturation , proliferation ,

pluripolericy, self – maintainance and self – renewal –problems in measuring stem cells – preservation protocols.

UNIT- II

STEM CELL CONCEPT IN PLANTS 10Hours Stem cell and founder zones in plants – particulary their roots – stem cells of shoot meristems of higher plants.

UNIT- III

STEM CELL CONCEPT IN ANIMALS 10Hours

Skeletal muscle stem cell – Mammary stem cells – intestinal stem cells – keratinocyte stem cells of cornea – skin and hair

follicles –Tumour stem cells, Ebryonic stem cell biology - factors influencing proliferation and differentiation of stem cells

– hormone role in differentiation.

UNIT- IV

HAEMOPOIETIC STEM CELL 10Hours Biology – growth factors and the regulation of haemopoietic stem cells.

POTENTIAL USES OF STEM CELLS

Cellular therapies – vaccines – gene therapy – immunotherapy – tissue engineering –blood and bone marrow – Fc cells.

TOTAL : 40 Hours

TEXT BOOKS

1. Stem cells – Elsevier : CS Potten , 1997.

2. Essentials of stem cell biology , Robert Paul Lanza ,

2006.

3. encyclopedia of stem cell research , volume 1 Clive Svendensen , Allison D.Ebert.



down to 45 marks.








UBT508L: BIOINFORMATICS LAB

1.5 Credits (0-0-3)

LIST OF EXPERIMENTS IN BIOINFORMATICS LABORATORY

1. Bibliographic search from PUBMED, SCIRUS and MEDMINER

2. Sequence retrieval from Nucleic acid and Protein databases.

3. Sequence searches using BLAST – Retrieval of homologs, paralogs, orthologs, and Xenologs

4. Pair wise comparison of sequences – Analysis of parameters affecting alignment.

5. Multiple alignments of sequences and pattern determination using PROSITE

6. Evolutionary studies / Phylogenetic analysis – Analysis of parameters affecting trees.

7. Identification of functional sites in Genes / Genomes.

8. Secondary structure prediction of proteins and comparison with PDB.

9. Restriction mapping: Analysis of maps for suitable molecular biology experiment.

10. Primer Design: Factors affecting primer design.

11. PDB structure retrieval and visualization: Analysis of homologous structures.

12. Determination of ligand-protein interactions using SPDBV/ LIGPLOT

13. Superposition of structures – Calculation of RMSD.

14. Docking studies – Analysis of substrate / ligand binding using homologous structures.

REFERENCE BOOKS

1. Bioinformatics – Andreas D Boxevanis. Wiley Interscience, 1998.

2. Bioinformatics – David W Mount, cold spring harbor, 2001.

3. Bioinformatics – A biologists guide to biocomputing and the internet. Stuart M brown, NYU

Medical Center, NY USA. 2000.

4. Analytical Tools for DNA, Genes & Genomes – Arseni Markoff, New Age, 2007.

5. Discovering Genomics, Proteomics & Bioinformatics – A M Campbell & L J Heyer, Pearson

Education, 2007

6. Fundamental Concepts of Bioinformatics – D E Krane & M L Raymer, Pearson, 2006.

7. Computational methods in Molecular Biology – S.L.Salzberg, D B Searls, S Kasif, Elsevier, 1998.

8. Bioinformatics – methods and applications: Genomics, proteomics and drug Discovery – s c

Rastogi, N. mendiratta & prastogi, phi, 2006

9. Introduction to Bioinformatics – Arthur Lesk, Oxford, 2006

LABORATORY ASSESSMENT

1) Each laboratory subject is evaluated for 100 marks (50 CIE and 50 SEE)

2) Allocation of 50 marks for CIE

Performance and Journal write-up: marks for each experiment = 30 marks/No. of proposed experiments.

One practical test, for 20 marks (5 write-up, 10 conduction, calculation, Result etc., 5 –viva-voce)

3) Allocation of 50 marks for SEE,

Major and Minor : 35

(Write-up 25%, conduction 50%, calculation and results 25%)

Spotting : 08

Viva-Voce : 07

UBT509L: GENETIC ENGINEERING LABORATORY

1.5 Credits (0-0-3)

LIST OF EXPERIMENTS IN GENETIC ENGINEERING LABORATORY

1. Transformation.

2. Blue white colony screening.

3. Thermal denaturation of DNA.

4. Restriction Digestion.

5. Restriction mapping of plasmid.

6. Ligation Experiment.

7. Southern blotting.

8. Western blotting.

9. UV survival curve of E.coli or any other bacteria.

10. Study of repair mechanism for damage caused by UV radiation(Demo expt)

11. PCR(Amplification with specific primers).

REFERENCE BOOKS

1. Principles of Gene manipulation-An introduction to genetic engineering, Old

R.W.,PrimroseS.B.,Blackwell Scientific Publications,1993.

2. Genetic Engineering Vol.1-4(Williamson Edition)

3. Current protocols in molecular biology-Greena Publishing Associates,NY,1988

4. Molecular cloning Volumes I,II,III-Sambrook J et al (2000).Cold Spring Harbor

Lab, 2000.

5. Introduction to Genetic Engineering-Sandhya Nair


1) Each laboratory subject is evaluated for 100 marks (50 CIE and 50

SEE)







Spotting : 08

Viva-Voce : 07

B.E. VI SEMESTER

UBT612C: BIOTRANSFORMATION AND ENZYME TECHNOLOGY

4 Credits (4-0-0)

Prerequisites : Biology, biochemistry and enzymology.

Course Objectives:

To know the difference between chemical catalyst and biocatalyst.

To understand the enzymatic transformation.

To know the importance of enzymes in diagnostics.

To study the immobilization techniques.

To know the applications of enzymes in industries.

To design and construct artificial enzymes.

UNIT- 1

Biocatalysts 13 Hours

Biocatalysts, advantages of enzymes vs chemical catalysts, applications of biocatalysts in industry, medicine

and research analysis, Enzyme business in India and abroad.

Enzymatic transformation Reaction engineering for enzyme-catalyzed biotransformations. Catalytic antibodies. Biocatalysts from extreme

Thermophilic and Hyperthermophilic Archaea and Bacteria. Biotransformation of drugs.

UNIT- 2

Enzymes of biological importance 13Hours Importance of enzymes in diagnostics, enzymes as therapeutic agents. Enzyme pattern in diseases like in

Myocardial infarctions (SGOT, SGPT, & LDH). Use of isozymes as markers in cancer and other diseases.

Acetylcholinesterase, angiotensin converting enzyme (ACE), pseudocholinesterase, 5’- nucleotidase (5NT),

glucose-6-phosphate dehydrogenase (GPD).

UNIT- 3

Immobilization of enzymes and bioconversion processes 13 Hours Techniques of enzyme immobilization; design and configuration of immobilized enzyme reactions, use of

immobilized enzymes, immobilized enzymes in bioconversion processes, bioreactors using immobilized

enzyme.

UNIT- 4

Industrial uses of enzymes 13 Hours Enzymes used in detergents, use of proteases in food, leather and wool industries; methods involved in

production of glucose syrup from starch (using starch hydrolyzing enzymes), uses of lactase in dairy industry,

glucose oxidase and catalase in food industry.

Enzyme engineering

The design and construction of novel enzymes, artificial enzymes. Enzymes in immunoassay techniques. DNA

ligases and restriction enzymes.

Total: 52 hours

Course Outcomes:

Ability to differentiate between chemical catalyst and biocatalyst.

Ability to understand the biotransformation.

Acquire the knowledge of use of enzymes in diagnostics.

Apply knowledge of immobilization of enzymes.

Apply knowledge of using enzymes in industries.

Ability to design and construct artificial enzymes.

REFERENCE BOOKS

1. Enzymes Biochemistry , Biotechnology, Clinical Chemistry by Trevor Palmer, Horwood Publishing Ltd,

First East-West Press Edition 2004

2. Fundamentals Of Enzymology: The Cell And Molecular Biology Of Catalytic Proteins Nicholas C.

Price, Lewis Stevens Edition : 2000

3. Enzyme Technology by M.P. Chaplin and C. Bucke, Cambridge University Press Cambridge, 1990

4. Biocatalyst for Industry: J.S. Dordrick (1991), Plenum press, New york

5. Enzymes in Industry: Production and Applications W. Gerhartz (1990), VCH Publishers, New York

6. Principles of Enzymology for technological Applications (1993):

7. Biotechnology by B D Singh.

8. David L. Nelson and Michael Cox, “Lehninger Principles of Biochemistry” –4th

Edition



down to 45 marks.








UBT604C: BIOPROCESS EQUIPMENT DESIGN & DRAWING

4 Credits (4-0-0)

Prerequisites : Unit Operations, heat & mass transfer, Bioprocess principles and calculations.

Course objectives:

To Explain the different types heat transfer modes.

To Distinguish between notations and terminology of welding and pipe joints.

To know the different types of valves and joints.

To Distinguish between types of heat transfer equipments.

To understand the referring of the Perry’s chemical Engg handbook.

To Calculate the no of tubes,dia and different parameter of DPHE.

To Draw the sketch of DPHE.

To Calculate the dimensions of shell and tube heat exchangers.

To Apply the design aspects of by solving the problems.

To Design the fermentor.

To Design the agitated and jacketed vessels.

1. Notation & terminologies 12 Hours

Joints, welded joints, pipe joints, pipe and pipefittings, vessel openings (man holes, nozzles, drains), agitators,

valves, ball, NonRetum safety.

2. Process equipments 40 Hours

DETAILED PROCESS, MECHANICAL DESIGN OF THE FOLLOWING EQUIPMENTS:

a) Double pipe heat Exchanger

b) Shell & Tube Exchangers

c) Distillation Column (packed bed type)

d) Fermentors Vessels

e) Agitated and jacketed vessels

Total: 52 hours

Course outcomes:

Able to define the notations and terminology for welding and pipe

joints.

Able to draw the assemble of various values and joints.

Able to calculate the no of tubes,dia,and different parameter of

double pipe heat exchanger.

Able to calculate the dimensions of shell and tube heat exchangers.

Able to apply the design aspects of by solving the problems.

Able to evaluate the no of plates of distillation column.

Able to design the fermentor.

Able to design the agitated and jacketed vessels.

REFERENCE BOOKS:

1. Process Equipment Design by M V Joshi

2. Unfired pressure vessel by I S Code

3. Chemical Engineers Handbook by Perry & Green

4. Process Equipment & Mechanical Aspects by V C Bhattacharya

5. Mechanical Equipment Design by Brownell & Young

6. Fermentation & Biochemical Engineering Hand Book (1983),

7. Principles, Process Design and Equipment. HC Vogel, Noyes. Coulson & Richradson, Vol. 6

8. Equipment Design by S Mahajan

9. Equipment Design by Atkins



down to 45 marks.








UBT602C: BIOPROCESS CONTROL & AUTOMATION

4 Credits (4-0-0)

Prerequisites :

Course Objectives:

To Explain the different types of units and dimensions.

To Distinguish between Rayleigh’s method and Buckingham’s Pi- theorem.

To Outline the procedure for Rayleigh’s method and Buckingham’s Pi- theorem.

To Distinguish between types of fluids, properties of fluids, and flow of fluids.

To Derive the Pascal’s law, Hydostatic law Bernoulli’s theorem.

To Calculate the pressure, kinetic and potential energy.

To Explain the different types of flow measurement equipment.

To Calculate power no calculations of mixing and agitations.

To Analyze the stoke’s law and newton’s law explanations.

To Estimate the minimum thickener area by conducting Batch sedimentation test.

To Calculate the particle size and various calculation by sieve analysis.

To Explain the different types, principle of size reduction equipments.

To Distinguish between crushers and grinders.

UNIT- 1

13 hours Instrumentation, Introduction to flow, pressure, temperature and level measurements, methods of on-line and

off-line measurements of cells, substrates and products, microbial caloriemetry, parameter estimation

techniques for biochemical processes. Introduction to Laplace Transformation, I order system - examples,

mercury in glass theromometer.

UNIT- 2

13 Hours

Response of 1st order system for sinusoidal input. Liquid level system, lineraisation, composition, I order

system in series, interacting and non-interacting systems. Second order system with under damping, derivation

of transfer function for various systems, dead time response of I and II order overdamped and underdamped

systems, to step, ramp, impulse (pulses) and sinusoidal changes.

UNIT- 3

13 hours

Closed loop control system, TFs for controller and various components of a control system, Control valve,

principle, components and their functioning, On-off controller, Propotional (P) controller, Derivative (D) and

Integral (I) controller, Transient responses for P, PI and PID controllers, Servo and Regulatory problems with

block diagrams, Reduction of block diagrams.

UNIT- 4

13 hours

Frequency response, Concept of stability, Routh test, Root locus diagram, Instrumentation and control of bio-

reactors and sterilizers, Flow injection analysis for measurement of substrate, products and other metabolites,

On-line and off-line biomass estimation, State and parameter estimation technique.

Total: 52 Hours

Course Outcomes:

Able to Explain the Instrumentation of flow, pressure, temperature.

Able to Derive the order of reactions for different types of inputs.

Able to Compare the different types of controllers.

Able to Explain the concepts of stability and routh test and root locus

diagram.

Able to Apply the design aspects of by solving the problems.

Able to Analyze the measurements of substrate' product and other

metabolites.

Able to Calculate the Block diagram and reduction in diagram.

Able to Derive the various transfer function for various systems.

REFERENCE BOOKS:

1. Biochemical Engineering Fundamentals by Bailey and Ollis, Mcgraw Hill (2nd Ed.). 1986.

2. Bioprocess Engineering by Shule and Kargi Prentice Hall, 1992.

3. Bioprocess Engineering Principles by Pauline M. Doran, 1995.

4. Chemical Process Control by Coughner.

5. Biotechnology and Bioprocess engineering: Proceedings - Edited by Tarun K Ghosh. VII international

Biotechnology Symposium. Delhi, 1984.

6. Wankat P.c. Rate controlled separations, Elsevier, 1990.



down to 45 marks.








UBT605H: BIOETHICS & BIOSAFETY

3 Credits (3-0-0)

Prerequisites : Basic and applied aspects of life science

Course Objectives:

To understand importance of bioethics and biosafety.

To understand legal social and economic impacts of biotechnology.

To understand regulatory guidelines and their importance.

To understand importance of patent.

To understand procedure to apply for patent.

To understand procedure of assesement of biosafety for biotech foods.

To understand ethical implications of biotechnology.

UNIT 1

Introduction to Bioethics & Biosafety: 10 Hours

Definition and scope of bioethics and biosafety, Ethical implications and need for biosafety, Legal and Socio-

Economic impacts of Biotechnology: Agriculture and Food sector, Institutional, social, Cultural, Business and

Consumer issues.

Biosafety regulation guidelines

Implications of biotechnological products and techniques, Bioterrorism, Objectives of safety guidelines,

Cartagena protocol on biosafety, Convention on biological weapons, Biosafety regulations national and

international guidelines with regard to rDNA technology, transgenic science,GM crops,

UNIT 2

Biosafety Regulation: 12 Hours

Genetically modified organisms and their release in environment. Experimental protocol approvals, Laboratory

associated infections and other hazards, Risk groups and assessment and levels of bisafety. Containments;

Physical, Biological. Guidelines for research in transgenic plants, manufacturing practices and good lab

practices (GLP &GMP). Pesticidal preharvest residue management

Intellectual Property Rights and Patenting

Introduction to IPR, Patents: patent laws &its principles in US, UK & in India, Criteria for patentability.

International agreements related to pharma, microbial, environmental, agricultural and informatics sectors with

case studies.

UNIT 3

10 Hours

Procedure to apply patent, other intellectual properties viz copy rights, Rights, Plant breeder’s rights, trade

secrets/ trade symbol etc. WTO, TRIPS, PCT and GATT. IPR problems and its hindrance.

Biodiversity

Convention on biodiversity, Indian Biodiversity act, Legal implications, Biodiversity and farmers rights. Role

of public and NGO’s in protection of biodiversity, conservation and sustainable use of biodiversity.

Human genome project and stem cells research: Introduction, Ethical, legal and social implications of HGP. Stem Cell Research- biosafety and its ethical issues

UNIT4

Food and pharma safety: 10 hours The GM-food debate and biosafety assessment procedures for biotech foods & related products, including

transgenic food crops, case studies of relevance. Environmental aspects of biotech applications. Special

application of patent laws in biotechnology and case studies. Flavr Savr Tomato as model case.Recombinant

organisms and transgenic crops, case studies of relevance (Eg. Bt cotton, Bt brinjal). Licensing and cross

licensing. Biosafety assessment of biotech pharmaceutical products.

Total: 42 Hours

Course outcomes :

Ability to Understand scope and aspects of Bioethics and biosafety,

Ability to understand ethical implications of biotechnology and need for biosafety.

Ability to understand biosafety regulation guidelines

Ability to understand analyze the public perception and public issues

regarding Biotechnology.

Ability to understand analyze safer use of Biotechnology in Agriculture,

Animal husbandry, Pharma, and Environment by implanting biosafety

regulations.

Ability to understand and analyze market strategies, status for BT products,

and to understand the concept of IPR.

Ability to understand biosafety assessment procedure for biotech food and

other products.

Ability to understand procedure to apply patent and its problems.

REFERENCE BOOKS:

1. Bioethics and Biosafety by Sateesh M.K.,I.K.International pub,2010

2. Biotechnology-expanding horizons by Singh B.D, Kalyani Pub, 2010

3. Biotechnology and Safety Assessment by Thomas, J.A., Fuch, R.L. (2002), Academic Press.

4. Biological safety Principles and practices) by Fleming, D.A., Hunt, D.L., (2000). ASM Press

5. Biotechnology - A comprehensive treatise. Legal economic and ethical dimensions VCH

6. Bioethics by Ben Mepham, Oxford University Press, 2005.

7. Bioethics & Biosafety by R Rallapalli & Geetha Bali, APH Publication, 2007



down to 45 marks.







3. Any Five Full questions to be answered choosing at least one from each unit

UBT621E: MICROBIAL BT

3 Credits (3-0-0)

Prerequisites : Basic and applied aspects of life science and microbiology.

Course Objectives:

To study about Genetic Transfer in bacteria cloning techniques.

To study industrial microbiology.

To study production & Biosynthesis microbial by products.

To know Uses of Bacteria in Bioremediation.

UNIT- 1

MICROBIAL BIOTECHNOLOGY 10 hours

a) In Bacteria: Genetic Transfer in bacteria, Transformation, Conjugation, Translation, cloning techniques,

polymerase chain reaction, expression of cloned Genes, Recovery and purification of expressed proteins.

b) In Yeast: Introduction of DNA into yeast cells, yeast cloning vectors, expression of foreign genes in yeast,

expression of foreign gene products in secreted form.

UNIT- 2

INDUSTRIAL MICROBIOLOGY 10hours

Vitamins as laxatives and analgesics; non steroidal contraceptives, external antiseptics, antacids and others.

Antibiotics and hormones. Impact of Biotechnology on vaccine development; sub unit vaccines, fragments of

antigen sub unit as synthetic peptide vaccines. Production of Microbial enzymes, strain -medium, fermentation

processes. Large scale application of Microbial enzymes - starch processing, textile designing, detergents,

cheese industry.

UNIT- 3

MICROBIAL BY PRODUCTS 10 hours

Bacillus thuringinesis, Sphaericus, Popilliae, Baculoviruses. Bacterial Polysaccharides - structure & role in

nature xanthan Gum - structure, production & Biosynthesis polyesters. Saccherification & fermentation.

Metabolites from microorganisms, Amino acids, antibiotics. Organic synthesis & Degradation, classification of

enzymes, microbial transformation of steroids & sterols.

ENVIRONMENTAL MICROBIOLOGY: Sewage & Waster water microbiology, Microbiological

Degradation of xenobiotics microorganisms in mineral recovery microorganisms in the removal of heavy metals

from aqueous effluents.

FOOD MICROBIOLOGY: Microbial spoilage of food and its control; food preservatives; fermented foods;

single cell protein (SCP) and single cell oil (SCO); food borne infections and their control.

UNIT- 4

BIOREMEDIATION AND BIOLEACHING 10 hours Uses of Bacteria in Bioremediation – Biodegradation of hydrocarbons, Granular sludge consortia for

bioremediation, crude oil degradation by bacteria, Immobilization of microbes for bioremediation,

Methanotrophs, PCB dechlorination, Genetic engineering of microbes for bioremediation. Phytoremediation –

plants capable of assimilating heavy metals. Studies of Pyrite Dissolution in Pachuca Tanks and Depression of

Pyrite Flotation by Bacteria, Factors Effecting Microbial Coal Solubilization, Sulfur Leaching by Thermophilic

Microbes of Coal Particles Varying in size, Microbiological Production of Ferric Ion for Heap and Dump

Leaching, New Bacteriophage which infects Acidophilic, Heterotrophic Bacteria from Acidic Mining

Environments, Treatment of Coal Mine Drainage with Constructed Wetlands.

Total 40 hourse

Course outcomes:

Able to study about Genetic Transfer in bacteria cloning techniques.

Able to study industrial microbiology.

Able to study production & Biosynthesis microbial by products.

Able to know Uses of Bacteria in Bioremediation.

REFERENCE BOOKS

1. Fundamentals of Biotechnology. Edited by Paule Prave, Uwe Faust, Wolfgang Sitting and Dieter A

Sukatsch. VCH Publishers.

2. Principles of fermentation Technology, P.F. Stanbury and A. Whitaker, Pergamon Press, 1984.

3. Alexander N Glazer, Hiroshi Nikaido by Microbial Biotechnology, W H Freeman & Company New York.

4. Microbiology by Bernard Davis & Renato Dulbecco, Lippincott Company, Philadelphia.

5. Principle of Microbe & Cell Cultivation (1975), SJ Prit, Blackwell Scientific co).



down to 45 marks.









UBT622E: GENOMICS AND PROTEOMICS

3 Credits (3-0-0)

Prerequisites : Cell biology & Genetics, Molecular biology, Genetic Engg. & Bioinformatics.

Course Objectives:

To describe how genomic DNA contains long stretches non-coding regions.

To describe how a single gene can give rise to multiple proteins.

To harness the emerging genomic,transcriptomic, proteomic and bioinformatics information to build

novel paradigms of biological importance.

UNIT- 1

Introduction: 10 Hours

Genes and Proteins, Polymorphisms – types of polymorphism, commercializing the Genome -Revenue

opportunities: a) genome sequences and database subscriptions, b) prediction of new genes and their function by

databases.

Sequencing & genome projects:

Early sequencing efforts. Methods of preparing genomic DNA for sequencing, DNA sequence analysis

methods, Sanger Dideoxy method, Fluorescence method, shotgun approach. Next generation sequencing

Genome projects on E.coli., Arabidopsis and rice; Human genome project .

UNIT- 2

Genomics: 10Hours

Gene variation and Single Nucleotide Polymorphisms (SNPs), Expressed sequenced tags (ESTs), genotyping

tools -DNA Chips, comparative genomics. Functional genomic studies with model systems such as Drosophila,

Yeast or C. elegans.

Genome management in eukaryotes:

Cell differentiation and gene regulation. Inheritance pattern in eukaryotes, Mutations, organization of

eukaryotic genome within the nucleus, translation and post-translational modification in eukaryotes.

Interference RNA, RNA silencing, SiRNA: Applications in Functional genomics, medicine and Gene

Knockdown. Metagenomics- definition & concept.

UNIT- 3

Functional genomics: 10Hours

C-Value and paradox of genomes, Repetitive and coding sequences, Genetic and physical maps, chromosome

walking. Molecular markers – RFLP, RAPD and AFLP, Microsatellites and telomerase as a molecular markers.

Methods of molecular mapping, Marker assisted selection, map based cloning, T-DNA tagging, Transposon

tagging. Bioinformatics analysis- clustering methods. Approaches to physical mapping, FISH – DNA

amplification markers.

UNIT- 4

Proteomics: 10 Hours

Introduction to proteins, Methods of protein isolation, purification, quantification, Large scale preparation of

proteins, use of peptides in biology, Proteomics databases and proteins as drugs.

Proteome analysis

Mass-spec based analysis of protein expression and post-translational modifications. "Protein Chip" -

interactions and detection techniques. Methods of measurement of mRNA expression, DNA array

hybridization Non-DNA array hybridization, two dimensional PAGE for proteome analysis, Applications of

proteome analysis to drug development and toxicology.

Total: 40 Hours

Course Outcomes:

Able to study genomes of organisms.

Able to study genes of the model organisms.

Analysis of the genomes and proteomes.

Able to analyze tools algorithms and technique for DNA sequencing.

REFERENCE BOOKS

1. Introduction to Genomics – Arthur M Lesk, Oxford University Press, 2007.

2. Plant Genome Analysis – Peter M Gresshoff, CRC Press.

3. Genetic Analysis – Principles, Scope and Objectives by JRS Finchman, Blackwell Science,1994.

4. Discovering Genomics, Proteomics & Bioinformatics – A M Campbell & L J Heyer, Pearson

Education, 2007

5. Protein Arrays, Biochips and Proteomics – J S Albala & I Humprey-Smith, CRC Press, 2003

6. Genomics & Proteomics – Sabesan, Ane Books, 2007.

7. Proteomics – S. R. Pennington and M J Dunn, 2004.

8. Purifying Proteins for Proteomics – Richard J Simpson, IK International, 2004

9. Proteins and Proteomics – Richard J Simpson, IK International, 2003

10. DNA sequencing – Luhe Alphey, 2004

11. Biocomputing Informatics and the Genome Projects – Smith D.W., Academic Press, 1993.

12. Genes VIII – Benjamin Lewis. Oxford University & Cell Press.

13. Bioinformatics –methods and applications: genomics, proteomics and drug discovery S C

RASTOGI, N MENDIRATTA & P RASTOGI, PHI, 2006

14. Genomics and evolution of Eukaryotes – Laura Katz and D Bhattacharya, 2007.



down to 45 marks.









UBT623E: PLANT BT

3 Credits (3-0-0)

Prerequisites : Cell biology, genetics and microbiology.

Course Objectives:

To study plant genetic engineering and transformation technology.

To study Application of plant transformation for productivity and performance

To study Metabolic engineering and industrial products.

To study nitrogen fixationand Identification of elite species and mass production for practical

application of algae.

UNIT- 1

PLANT GENETIC ENGINEERING 10 Hours Induction of tumours by Agrobacterium, introduction of binary vectors into Agrobacterium by triparental

mating, leaf disc transformation using Agrobacterium, GUS expression in transformed tissues, extraction of

DNA from transformed plants, Southern hybridization to check plant

22 transformation, PCR amplification of T-DNA in transformed plant tissues. Agrobacterium mediated gene

transfer and cloning. Types of plant vectors and their use in gene manipulation. Viruses as a tool to delivery

foreign DNA.

TRANSFORMATION TECHNOLOGY Plant transformation technology -Basis of tumor formation, hairy root, features of Ti and Ri plasmids,

mechanisms of T-DNA transfer, role of virulence genes, use of Ti and Ri-plasmids as vectors, binary vectors.

Vectorless or direct DNA transfer-particle bombardment, electroporation, microinjection, transformation of

monoctos. Mechanism of transgene interaction - Transgene stability and gene silencing. Generation and

mainteance of transgenic plants.

UNIT- 2

APPLICATIONS 10 Hours

Application of plant transformation for productivity and performance – Herbicide resistance phosphoinothricin,

glyphosate, atrazine, insect resistance -bt genes, Structure and function of Cry proteins – mechanism of action,

critical evaluation of its impact in on insect control. Non-bt like protease inhibitors, alpha amylase inhibitor,

virus resistance -coat protein mediated, nucleocapsid gene, disease resistance -chitinase, 1-3 beta glucanase,

RIP, antifungal proteins, thionins, RS proteins, abiotic stress – drought and salinity, post-harvest losses, long

shelf life of fruits and flowers, use of ACC synthase, polygalacturanase, ACC oxidase, male sterile lines, barstar

and barnase systems.

UNIT- 3

SECONDARY METABOLITES & GENE MARKERS 10 Hours Metabolic engineering and industrial products -Plant secondary metabolites. Industrial enzymes, biodegradable

plastics, polyhydroxybutyrate, antibodies, edible vaccines. Molecular marker-aided breeding -RFLP maps,

linkage analysis, RAPD markers, STS, microsatellites, SCAR (sequence characterized amplified regions), SSCP

(single strand conformational polymorphism), AFLP, QTL, map-based cloning, molecular marker assisted

selection.

UNIT- 4

NITROGEN FIXATION 10 Hours Nitrogen fixation and biofertilizers -Diazotrophic microorganisms, nitrogen fixation genes. Two component

regulatory mechanisms. Transfer of nif genes to non-diazotrophic microorganisms, nod genes structure function

and role in nodulation, Hydrogenase -Hydrogen metabolism. Genetic engineering of hydrogenase genes.

ALGAE

Blue-green algae and Azolla -Identification of elite species and mass production for practical application.

Mycorrhizae -importance in agriculture and forestry. Algae as a source of food, feed, single cell protein,

biofertilizers; industrial uses of algae. Mass cultivation of commercially valuable marine macroalgae for agar

agar, alginates and other products of commerce and their uses. Mass cultivation of microalgae as a source of

protein and feed. 6 Hour

Total: 40 Hours

Course outcomes: Students able to

study plant genetic engineering and transformation technology.

study Application of plant transformation for productivity and performance

study Metabolic engineering and industrial products.

study nitrogen fixationand Identification of elite species and mass production for practical application of

algae.

REFERENCE BOOKS

1. Plant Cell Culture: A Practical Approach by R.A. Dixon & Gonzales, IRL Press.

2. Plant biotechnology in Agriculture by K. Lindsey and M.G.K. Jones (1990), Prentice hall, New Jersey.

3. Plant Biotechnology 1994, Prakash and Perk, Oxford & IBH Publishers Co J Hammond, P McGarvey and V

Yusibov (Eds): Plant Biotechnology. Springer Verlag, 2000

4. HS Chawla: Biotechnology in Crop Improvement. Intl Book Distributing Company, 1998

5. Biodegradation and Detoxification of Environmental Pollutants – Chakrabarthy AM RJ Henry: Practical

Application of Plant Molecular Biology. Chapman and Hall 1997

6. Plant Tissue Culture: Applications and Limitations by S.S. Bhojwani (1990), Elsevier,

Amsterdam. TJ Fu, G Singh and WR Curtis (Eds):

7. Plant Cell and Tissue Culture for the Production of Food Ingredients. Kluwer Academic Press, 1999

8. PK Gupta: Elements of Biotechnology. Rastogi and Co. Meerut 1996

9. Biotechnology in Agriculture, MS Swamynathan, McMillian India Ltd. Gene Transfer to Plants 1995

10. Polyykus I and Spongernberg, G.Ed. Springer Scam Genetic Engineering with Plant Viruses, 1992 T

Michael, A Wilson and JW Davis, CRC Press.

11. Molecular Approaches to Crop Improvement 1991. Dennis Liwelly Eds. Plant Cell

12. and Tissue Culture-A Laboratory mannual 1994. Reinert J and Yeoman MM, Springer.



down to 45 marks.









UBT624E: ANIMAL BT

3 Credits (3-0-0)

Prerequisites : Cell biology, molecular biology and microbiology.

Course Objectives:

To study cell lines and cell culture

To study invitro fertilization & cloning.

To study human genome and Transgenic animals

To know Application of animal cell culture

UNIT- 1

CELL LINES 10 Hours

Primary culture – Mechanical and enzymatic mode of desegregation, establishment of primary culture.

Subculture -passage number, split ratio, seeding efficiency, criteria for subculture. Cell lines -definite and

continuous cell lines, characterization, authentication, maintenance and preservation of cell lines.

Contamination -bacterial, viral, fungal and mycoplasma contaminations, detection and control, cell

transformation – normal vs. transformed cells, growth

CELL CULTURE

Scale-up of animal cell culture – Factors to be considered. Scale-up of suspension cultures Batch reactor,

continuous culture, perfusion systems. Scale-up of monolayer cultures – roller bottles, Nunc cell factory,

microcarrier cultures, organotypic culture, matrices, factors affecting culture and perspectives.

UNIT- 2

INVITRO FERTILIZATION & CLONING 10 Hours

Conventional methods of animal improvement, predominantly selective breeding and crossbreeding. Embryo

biotechniques for augumentation of reproductive efficiency and faster multiplication of superior germ plasm.

Super ovulation Oestrus synchronization. Embryo collection, evaluation and transfer. Invitro maturation of

oocytes. Invitro fertilisation and embryo culture. Embryo preservation. Micro manipulation and cloning.

Artificial insemination, preparation of foster mother, surgical and non-surgical methods of embryo transfer,

donor and recipient aftercare. Cloning -concept of nuclear transfer, nuclear reprogramming and creation of

Dolly. Stem cells -embryonic and adult stem cells, plasticity and concept of regenerative medicine.

UNIT- 3

HUMAN GENOME 10 Hours Human genome complexicity of the genome, outlines of human genome project, human disease genes.

Molecular biological techniques for rapid diagnosis of genetic diseases. Chemical carcinogenesis, transfection,

oncogenes and antioncogenes. Cryo preservation and transport of animal germ plasm (i.e. semen, ovum and

embryos). Genetherapy -ex vivo and in vivo gene therapy methods, applications.

TRANSGENICS

Transgenic animals -retroviral, microinjection, and engineered embryonic stem cell method of transgenesis.

Application of transgenic animals -biopharming, disease models, functional knockouts.

UNIT- 4

OTHER APPLICATIONS 10 Hours

Application of animal cell culture -Vaccine production, specialized cell types. Concepts of tissue engineering -

skin, liver, kidney, bladder and heart. Principles and species suitable for aquaculture (Indian major carps and

prawns). Genetic status of culture stocks. Chromosome manipulations -Production of all male and sterile

populations, Hypophysation in fishes and prawns. Pearl culture -pearl producing mollusks, rearing of oysters,

nucleation for pearl formation and harvesting of pearls. Probiotics and their significance in aquaculture.

Molecular tools for the identification of diseases in aquatic species.

Total: 40 Hours

Course outcomes: Students able to

study cell lines and cell culture

study invitro fertilization & cloning.

study human genome and Transgenic animals

know Application of animal cell culture

REFERENCE BOOKS

1. Culture of Animal Cells, (3rd Edn) R Ian Fredhney. Wiley-Liss Animal Cell Biotechnology, 1990- Spier,

RE and Griffith, JB Academic Press, London

2. Animal Biotechnology by Murray Moo-Young (1989), Pergamon Press, Oxford Animal Cell Technology,

Principles and practices, 1987, Butter, M Oxford press

3. Molecular Biotechnology by Primrose.

4. Methods in Cell Biology, Vol. 57, Animal Cell Culture Methods Ed. JP Mather and D Bames. Academic

Press Fish and Fisheries India VG Jhingram

5. Living resources for Biotechnology, Animal cells by A. Doyle, R. Hay and B.E. Kirsop (1990), cambridge

University Press, cambridge.

6. Animal Cell Culture – Practical Approach, Ed. John RW. Masters, Oxford Animal

7. Cell Culture Techniques Ed Martin Clynes, Springer Cell Culture Lab Fax. Eds. M

8. Butler & MDawson, Bios Scientific Publications Ltd. Oxford



down to 45 marks.









UBT625E: BIOFUELS TECHNOLOGY

3 Credits (3-0-0)

Prerequisites : Biochemistry, Cell biology, Microbiology.

Course Objectives:

To study the bioconversion process in biofuel production.

To know about the biofuel life cycle and its advantages & disadvantages.

To study about the types of feedstocks used for biofuel production.

To know about the different types of biofuels and their properties.

To understand the technologies used for biofuel production.

To know about the issues related with biofuels and its market barriers.

UNIT- 1

Biochemistry of biofuels and energy resources: 10 Hours

Basic principle of light energy conversion to chemical energy &carbon fixation. Biochemistry involved in

conversion of sugars to alcohols. Renewable and non-renewable resources.

Biofuels

Introduction to Biofuels - definition, advantages and disadvantages. Biofuel life cycle. Biomass as an energy

core and its different mode of utilization. Conventional fuels and their environmental impacts. Modern fuels and

their environmental impacts. Biofuel energy content. World scenario of biofuel production and use.

UNIT- 2

Biofuel feed stocks 10 Hours Starch feed stocks-cereal grains, tubers & roots; Sugars feed stocks-sugarcane & sugarbeet; cellulosic feed

stocks - forest residues, agricultural residues, Agricultural processing by-products, dedicated energy crops,

municipal solid waste and paper waste. Lipid feedstocks :-Oilseed crops with examples, Algae, Waste oil,

Animal fats. Next generation feed stocks. Environmental impacts of feed stocks.

Types of biofuels

First generation biofuels-vegetable oil biodisel, bioalcohols, bioethers, biogas syngas, solid biofuels. Second

generation biofuels and third generation biofuels,

UNIT- 3

Technologies for biofuels 10 Hours

Historical background. Biochemical platform – bioethanol production, standardization, emissions and properties

of bioethanol. Thermochemical platforms - biodiesel production, standardization, properties and emissions of

biodiesel. BtL fuels -production, properties and emissions. Biohydrogen processing and uses. Converting solid

wastes to pipeline gas. Biomethanation, Microbial fuel cells. Blending of biofuels

UNIT- 4

Biofuels in perspective 10 Hours

Integrated refining concepts with reference to ethanol production. Economic feasibility of producing biodisel,

Issues with biofuel production & use. Impact of biofuel in global climate change & food production. 1st versus

2nd

generation biofuels.. Strategies for new vehicle technologies. Current research on biofuel production.

Market barriers of biofuels.

Total: 40 Hours

Course Outcomes:

Ability to understand the bioconversion process in biofuel production.

Able to know biofuel life cycle.

Able to know types of feedstocks used for biofuel.

Able get the knowledge about the technologies used for biofuel production.

Able to know the issues related with biofuels.

REFERENCE BOOKS

1. Environmental Biotechnology by Foster C. F., John ware D.A., Ellis Horwood Limited, 1987.

2. Fuels from Waste by Larry Anderson and David A Tillman. Academic Press, 1977.

3. Biofuels by Ayhan Demirbas publ. Springer

4. Biofuels (Series - Energy For The Future And Global Warming)

5. Biotechnology, Economic & Social Aspects: E.J. Dasilva, C Ratledge & A Sasson,

Cambridge Univ. Press, Cambridge.

6. Environmental Biotechnology by Pradipta Kumar Mahopatra, 2007.


1. CIE comprises of 3 tests, each of 30 marks and I hr duration,totaling to 90 marks and later is scaled down to

45 marks.








UBT628E TRANSPORT PHENOMENA

3 Credits (3-0-0)

Prerequisites : Unit Operations,HMT

Course Objectives:

To study Momentum transfer – Principles and Applications.

To study Mechanisms of heat transfer and Mass Transfer.

To study different Separation Processes.

UNIT- 1

Momentum Transfer and Overall Balances: 10 Hours

Fluid Statics, General molecular transport equations for momentum, heat and mass transfer, Viscosity of fluids,

Overall balances: mass balance/continuity equation, energy balance, momentum balance, shell momentum

balance and velocity distribution in laminar flow, design equation for laminar and turbulent flow in pipes.

Momentum transfer – Principles and Applications: Flow past immersed objects, packed beds, Non-

Newtonian fluids, Differential equations of continuity, momentum transfer (motion).

UNIT- 2

Steady State Heat Transfer: 10 Hours Mechanisms of heat transfer, conduction – through solids in series, steady state conduction and shape factors,

Forced convection - heat transfer inside pipes, natural convection heat transfer, boiling and condensation, heat

exchangers.

Unsteady State Heat Transfer: Derivation of basic equation, simplified case for systems with negligible

internal resistance.

UNIT- 3

Mass Transfer: 10 Hours

Mass transfer and diffusion, molecular diffusion in gases, liquids and solids. Mass transfer coefficients.

Separation Processes - Evaporation, Drying, Humidification, and Absorption.

UNIT- 4

Separation Processes: 10 Hours

Distillation, Adsorption, Ion Exchange, Leaching, Crystallization, Membrane processes.

Total 40 Hours

Course Outcomes:

Able to understand Momentum transfer – Principles and Applications.

Mechanisms of heat transfer and Mass Transfer.

Separation Processes.

REFERENCE BOOKS

1. Transport Processes and Separation Process Principles – C. J. Geankoplis, 4th Edition.

2. Momentum, Heat and Mass Transfer – Bennett and Myers.

3. Introduction to Transport Phenomena – William J. Thomson, PHI.

4. Transport Phenomena – Bird, Stewart, Lightfoot, 2nd Edition, JWI.

5. Fundamentals of momentum, heat and mass transfer – Welty, Wicks and Wilson.

6. Fundamentals of Fluid Mechanics – Sawhney Gs (2008) IK Publishers

7. Unit Operations of Chemical Engg. – McCabe & Smith (M G H Publications), 6th

Edition 1999.

8. Principles of Unit Operations in Chemical Engg. – Geonklopins.

9. Fluid Mechanics – K L Kumar.

10.Mechanics of fluids – B.S. Massey.


1. CIE comprises of 3 tests, each of 30 marks and I hr duration, totaling to 90 marks and later is scaled

down to 45 marks.




QUESTION PAPER PATTERN of SEE

1.Total of Eight Question with Two from each unit to be set uniformly covering the entire syllabus.

2.Each question should not have more than four sub questions.

3.Any Five Full questions are to answered choosing atleast one from each unit.

UBT608L: BIOKINETICS & ENZYME TECHNOLOGY LAB

1.5 Credits (0-0-3)

LIST OF EXPERIMENTS IN BIOKINETICS & ENZYME TECHNOLOGY LAB

1. RTD in PFR

2. RTD in MFR

3. Isolation of alpha-amylase from sweet potato or saliva

4. Maltose calibration curve by DNS method

5. Determination of activity of Salivary alpha-amylase

6. Determination of Specific activity of an enzyme

7. Effect of pH and temperature on enzyme activity

8. Determination of Kinetics constants (Km & Vmax)

9. Urea calibration curve

10. Determine the activity of enzyme Urease

11. Effect of inhibitors on enzyme activity

12 Immobilization of enzyme and determination of immobilized enzyme activity

Prediction of error percentage, standard deviation need to be calculated from expt. no 5 and 6

Demo experiments:

1. Ammonium sulphate fractionation and desalting (G-25 column chromatography)

2. Molecular weight determination of a protein by molecular sieving

3. Molecular weight determination of a protein by gel electrophoresis

REFERENCE BOOKS:

1. Biochemical Engineering Fundamentals by Bailey and Ollis, Mcgraw Hill (2nd Ed.) 1986.


3. Wolf R. Vieth, Bioprocess Engineering – Kinetics, Mass Transport, Reactors and Gene Expression. A

Wiley – Interscience Publication, 1992.

4. Smith J.M. Chemical Engineering Kinetics, McGraw Hill, 3rd Edition, New Delhi,1981

5. Carbery J A. Chemical and Catalytic Reactor Engineering, McGraw Hill, 1976.

6. Enzymes in Industry: Production and Applications : W. Gerhartz (1990), VCH Publishers, New York.

7. Enzyme Technology by M.F. Chaplin and C. Bucke, Cambridge University Press, Cambridge, 1990.

8. Enzymes: Dixon and Webb. IRL Press.

9. Principles of Enzymology for technological Applications (1993): B Heinemann Ltd.

Oxford.









Spotting : 08

Viva-Voce : 07

UBT609L: BIOPROCESS CONTROL & AUTOMATION LAB

1.5 Credits (0-0-3)

LIST OF EXPERIMENTS IN BIOPROCESS CONTROL & AUTOMATION LAB

1. Characteristics of Transducers (Temperature).

2. Characteristics of Transducers (Pressure).

3. Characteristics of Transducers (Flow).

4. Dynamics of First order system for step input.

5. Dynamics of First order system for impulse input.

6. Non-interacting system.

7. Interacting System.

8. Control of temperature in a bioprocess.

9. Control of pH in a bioprocess.

10. Control of Pressure in a bioprocess.

11. Control of Flow rates in a bioprocess.

12. Measurement of dissolved oxygen in the growth media (at different stages of growth).

13. Measurements of temperature, light & humidity in growth chambers.

14. Fermenter Performance studies (Evaluation of Products).

REFERENCE BOOKS:


2. Bioprocess Engineering Principles by Pauline M. Doran, 1995.

3. McCabe W.L. and Smith J.C. Unit operations in Chemical Engineering, McGraw-Hill (5th

Ed.), 1987.

4. Bailey and Ollis, Biochemical Engineering Fundamentals, Mcgraw Hill (2nd

Ed.). 1986.

5. Wolf R. Vieth, Bioprocess Engineering – Kinetics, Mass Transport, Reactors and Gene Expression. A

Wiley - Interscience Publication, 1992.









UBT611L -BIOSTATISTICS LAB

2 Credits (0-0-4)

LIST OF EXPERIMENTS

1. Procedure for creating Data file, Diagram and Graphs.

2. Procedure and calculation of Mean, Median, Mode, Standard Deviation and Variance.

3. Procedure and calculation of t, Z and F test.

4. Calculation of Chi-square test.

5. ANOVA- one-way analysis

6. ANOVA- two-way analysis.

7. Experimental Research Design – CRD- Analysis.

8. Experimental Research design – RBD- Analysis.

9. Experimental Research design – Latin square- Analysis.

10. Calculation of Regression and correlation.

11. Multiple Regression Analysis.

12. Placket-Burman Design for media optimization.

13. Response Surface Methodology for media optimization.

REFERENCE BOOKS:

1. Marcello Pagano & Kimberlee Gauvreu, “Principles of Biostatistics” -Thompson Learning Pub, 2006.

2. Ronadd N Forthofer and Eun Sul Lee, “Introduction to Biostatistics” -Academic Press, 1995.

3. Agarwal B L., “Basic Statistics”-New Age International Pub, fifth Edn, 2009

4. Norman T J Bailey, “Statistical methods in Biology” -Cambridge Press, 1995.

5. Kapur J N., “Mathematical Models in Biology and Medicine”, 1st Edition, New age international Pvt. Ltd,

2001.

6. Khan and Khanum, Fundamentals of Biostatistics, Ukaaz pub,3 rd edn,2008

7. Rubinow S I., “Introduction to Mathematical Biology” -John Wiley, 1975.

8. Richard A. Johnson, “Miller & Freund’s Probability and statistics for engineers” Prentice Hall, 2000

9. Veer Bala Rastogi, “Fundamentals of Biostatistics” -Ane Books, 2006

10. Sabine Landau and Brian S. Everitt, A Hand Book of Statistical Analysis using SPSS, Chapman &

Hall/CRC.









Spotting : 08

Viva-Voce : 07

Documents

SCHEME OF TEACHING AND EXAMINATION B.E. V …SCHEME OF TEACHING AND EXAMINATION B.E. V SEMESTER (2014-15) Sl. No. Subject Code Subject Credits Hours/Week Examination Marks Lecture