Scanning em of C. elegans

Crawl movie

Fly-in movie

Male, scanning em

Male tail scanning em

Rene Mating Movie

Embryonic cell lineage

D lineage

ABala lineage

AIML neuron

AIML lineage

AIML circuit

Z1Z2 Z3 Z4

Postembryonic blast cells

Adult hermaphrodite anatomy

C. elegans typical cross section

Seam cell postembryonic lineages in the male

wild type

lin-4(e912) reiterations

wild type

lin-14(sd) repeats S1

lin-14(lf) skips S1

lin-4(e912) lin-14(sd)

lin-4(lf) and lin-14(gf) have the same phenotype: they repeat S1.

LIN-4S is complementary to sequences in the 3’UTR of lin14.

Human miRNA genes

Symmetric neuron phenotype of lsy-6(-)

A regulatory pathway leading to neuron asymmetry involving two micro RNA’s

Worms Eating Bacteria Expressing dsRNA to a GFP Reporter Gene Activate RNAi

wild type worms RNAi-defective mutants

GFP lost in embryosGFP lost in the gut

from Mello and Conte,Nature 431, 338-342.

RNAi in Plants

1928 report of developmentof viral (tobacco ringspot virus)resistance in tobacco, nowknown to be due to endogenousRNAi.

1928 report of developmentof viral (tobacco ringspot virus)resistance in tobacco, nowknown to be due to endogenousRNAi.

RNAi/miRNA Pathways

from Meister and Tuschl, Nature431, 343-349, 2004.

The Structure of Argonaute (Slicer)

Song et al., Science305, 1434-1437, 2004.