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Saliva Magnetic Bead v3

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Page 1: Saliva Magnetic Bead v3

Figure 1. Resolution of DNA Isolated from Preserved Saliva Samples.

HigH THrougHpuTSaliva DNa iSolaTioN KiT

NORGENBIOTEK CORP.The Sample Preparation Experts

T 1-866-NORGENBT 905-227-8848

F 905-227-1061E [email protected]

www.norgenbiotek.comISO 13485:2003, ISO 9001:2008,ISO 15189:2012 Registered

Magnetic Bead System — AutomationSaliva DNA can either be isolated from saliva samples collected and preserved using Nor-gen’s Saliva Collec-tion and Preservation Devic-es or fresh saliva samples. After a brief Protein-ase K incubation, Magnetic Bead Suspensions are added to the sample and then placed on the magnetic separation rack.

Only the DNA will bind to the magnetic beads, while most of the proteins will be removed in the superna-tant. The bound DNA is then washed in order to re-move any remaining impurities, result-ing in a high quality DNA elution.

DNAMicroarray

SNP AnalysisNext-GenSequencing

Genotyping

Application Ready DNAColumn

(Cat. RU45400)

M A B C D E F M

High Integrity DNA

Before After

Isolate high quality and quantity DNA from fresh, frozen or preserved saliva samples

Fast and easy processing using a magnetic bead system

High Integrity DNA (up to 50kb)

Consistent, high yields of genomic DNA

Product Features

Page 2: Saliva Magnetic Bead v3

HigH THrougHpuTSaliva DNa iSolaTioN KiT

Ordering Information

Figure 3. High Quality DNA Confirmed by Real-time PCR.Figure 2. Comparison of Saliva DNA Isolation Methods(Column vs. Magnetic Bead System)

v3.0

0

10

20

30

40

50

60

70

Column A B C D E F

Conc

entr

atio

n (n

g/μL

)

Description Prep Size Cat. Number

Saliva DNA Isolation Kit (Magnetic Bead System) 50 preps RU55400

Saliva DNA Isolation Kit 50 preps RU45400

Saliva Collection and Preservation Devices 50 units RU49000

Hierarchical Clustering Dendrogram

NORGENBIOTEK CORP.The Sample Preparation Experts Manufactured in our ISO accredited facility.

ISO9001:2008

ISO15189:2012

ISO13485:2003

Preserved human saliva samples Preserved human fecal samples

Figure 4. Hierarchical clustering of samples based on genus-level classifications, including a bar chart showing the relative abundance of genus-level classifications for each sample in the dendrogram.

Consistent Isolation of High Quality DNAComparable Concentration vs.

Column-based methods