Sakura webinar seriesCompetitor update Single slide stainer VENTANA HE 600

Anamaria Grigore

Strategic Product Manager Staining, Coverslipping and Digital Imaging

Sakura webinar series

Goal: Continue to extend and protect our leadingposition in routine Staining & Coverslipping

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• Post your question, a selection of the questions will be answered at the end

• You will receive a copy of this presentation

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Today’s presenter:

Name: Anamaria GrigoreFunction: Strategic Product Manager Staining, Coverslipping and Digital Imaging

Staining spectrum

Staining

Routine H&E

IHC

Special stainsDNA-RNA:

In situ hybridization

Staining spectrum

Staining

Routine H&E

IHC

Special stainsDNA-RNA:

In situ hybridization

Tissue groups or specific parts

confirm type and future treatment

Proteins on cell level, mostly used to specify

cancer type

Specific genes or dna defects

General morphology qualitative diagnosis

2 staining methods

Single slide staining

• Each slide treated like an individual specimen

Dip & Dunk (linear) staining

• Batch of slides immersed in a bath of staining solution

Single slide staining

Ventana HE600

Throughput & Time to first result

Drying oven

Customizable protocols

Ventilation needed

Power supply

Waste disposal

Eco friendly as option

Glass coveslipping

Recyclable reagent bottles

Caregiver

Values & benefits Single slide staining (Roche HE600)

Quality Safety Lean

Single slide stainer Close platform RFID technology

Recyclable reagent bottles No cross contamination due

to staining RFID technology

Validated protocol Change of reagents during a

run Caregiver remote support Minimum end user

maintenance

Dip and Dunk(linear)staining

Values & benefits Prisma Film

High throughput/speed 1 Preventive Maintenance

(PM) per year of 4 hours Standard power supply

Open platform (both H&E and special stains)

Coverslipping choice (glass of film)

Choice of reagents and slides

Proven technology High installed base >1000

units in SFE territory MTBR›52 weeks

Productivity Flexibility Reliability

Cross contamination

• Chance of cross contamination is very small (0.01% to 1.2% of slides)1

• Most contamination takes place at Grossing table and during Microtomy (water bath) Processing and Staining & Coverslipping has no significant impact. 2

• Conclusion: Changing to Single Slide Staining will not bring significant benefits to overcome cross contamination

Grossing Processing Embedding Microtomy Staining & Coverslipping

Truth or fiction:Cross contamination reduction

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1 Layfield LJ, et al, Extraneous Tissue A Potential Source for Diagnostic Error in Surgical Pathology, Am J Clin Pathol. 2011; 136(5):767-772)2 Gephardt GN, and Zarbo RJ., Extraneous Tissue in Surgical Pathology, Arch Pathol Lab Med. 1996; 120: 1009-1014

30% 60%

Truth or fiction:Cross contamination reduction

HE600 concept looks great, yet…

• Sponsored studies by Roche were performed with new charged slides ,not reflecting daily practice

• Chance of cross contamination in staining is very small (confirmed by many studies and customers)

• Most contamination takes place at grossing, embedding, microtomy

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What should the customer do?

Target customer groups

Target customers HE 600

• Innovators and early adopters ≈ 15% of the market

• Small and medium labs

• Symphony accounts for replacement

• Roche reference sites

• Customers scared of cross-contamination

Target customers Prisma & Film

• Early and late majority ≈ 70% of the market

• Small, medium and large labs

• DRS and SCA customers

• All customers that want/ need a reliable and flexible

solution for staining & coverslipping

Prisma & Film Value proposition

Value proposition

Small labs Flexibility/H&E and special stains in one/quality/reliability

Medium and large labs High throughput and speed/quality/reliability

Customers scared of cross-contamination Floaters represent a potential source of diagnostic error and occur in 0.01% to 1.2% of slides*Customers for Tissue-Tek AutoTEC a120, Autosection

All labs Productivity/Quality/Reliability

*A Potential Source for Diagnostic Error in Surgical Pathology Lester J. Layfield, MD,1 Benjamin L. Witt, MD,1 Kenneth G. Metzger, HTL(ASCP),2and Gina M. Anderson, HT(ASCP)2

Comparative analysis

Prisma & Film Roche HE 600

Throughput and speed √ -

Reliability √ Not yet proven

H&E & special stains & cytologyin one platform

√ -

Consistency of results √ √

Choice of reagents, slides, coverslipping method √ -

Environmental friendly (reagents and fumes) √ √

LIS connectivity√

√ only with VENTANA VANTAGE or VENTANA Connect Software

STAT in coverslipping √ -

Take away message

Continue to extend and protect our leadingposition in routine Staining & Coverslipping

Wrap-up

• You will receive a copy of this presentation and the publication on floaters and cross contamination.

• For unanswered questions please contact a.grigore@sakura.eu

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Thank you for attending thisSakura Academy webinar

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