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PHYTOCHEMICAL AND IN-VITRO STUDY OF ETHYL ACETATE EXTRACT OF

Spondias pinnata

A thesis report, submitted to the Department of Pharmacy, East West University, in partial

fulfillment of the requirements of the Degree of Pharmacy

Submitted by

Md. Nasidul Islam

ID 2008-3-70-098

Research Supervisor

Kh. Tanvir Ahmed

Senior Lecturer

Department of Pharmacy

East West University

Aftabnagor, Rampura, Dhaka.

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This Research Paper is dedicated to

Almighty ALLAH

The most Gracious

The most Merciful

&

To My Parents

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Declaration by the Research Candidate

I, Md. Nasidul Islam, hereby declare that the dissertation entitledPhytochemical and in-vitrostudy of methanolic extract of Spondias pinnata, submitted by me to the Department of

Pharmacy, East West University, Bangladesh, in the partial fulfillment of the requirement for the

award of the degree of Bachelor of Pharmacy is a bonafide record of original research work carried

out by me in 2012 under the supervision and guidance of Kh. Tanvir Ahmed, Senior lecturer,

Department of Pharmacy, East West University and it has not formed the basis for the award of any

other Degree/Diploma/Fellowship or other similar title to any candidate of any University.

Place

Dhaka

Signature of the Candidate

Md. Nasidul Islam

_______________________

Date: 23/ 12 / 2012 ID# 2008-3-70-098

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Certificate

This is to certify that the thesis submitted to the Department of Pharmacy, East West University,Bangladesh, in partial fulfillment of the requirement for the Degree of Bachelor in Pharmacy, was

carried out by Md. Nasidul Islam ID. 2008-3-70-098.

Sufia Islam, Ph.D

Chairperson and Associate Professor

Department of Pharmacy

East West University

Aftabnagor, Rampura, Dhaka

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Certificate

This is to certify that the thesis Phytochemical and in-vitro study of methanolic extract ofSpondias pinnata submitted to the Department of Pharmacy, East West University, Bangladesh, in

partial fulfillment of the requirement for the degree of Bachelor of Pharmacy was carried out by Md.

Nasidul Islam ID. 2008-3-70-098,under my guidance and supervision and that no part of the thesis

has been submitted for any other degree. I further certify that all the sources of information,

laboratory facilities availed of this connection is dully acknowledged.

Kh. Tanvir Ahmed

Senior lecturer

Department of Pharmacy

East West University

Aftabnagor, Rampura, Dhaka

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Acknowledgement

First, all praise and glory are to Allah for all the bounties granted to me and only with His

guidance and help this achievement has become possible.

I wish to express my thanks and gratitude towards my supervisorKh. Tanvir Ahmed, Senior

Lecturer, Department of Pharmacy, East West University, for his constant encouragement,

helpful discussions and able guidance throughout this study. His generosity with time and

constructive comments were of vital support and were above the call of duty.

I would also like to specially thankMd. Amran Howlader, Senior Lecturer, Department of

Pharmacy; Apurba Sarkar Apu, Senior Lecturer, Department of Pharmacy, East West

University for the continuous support. Their advice and instructions helped me to get the best

results.

I am indebted to Dr. Sufia Islam, Chairperson and Associate Professor, East West University,

for her kind support and the permission to use the facilities available at the Department of

Pharmacy, East West University, as well as for her encouragement to carry out research.

I would like to convey deepest love and obedience to my caring parents and elder sister fortheir support and guiding me all through my life until today, which keeps me strong and honest

to do the things I needed to do.

Finally I would also like to thank all the students especially Mohiuddin, Md.

Shamimuzzaman, Md. Mahfuz-e alam, A. K. M. Radowan Ahmad, Md. Mustakim Billah,

Md. Aminul Islam, Keya, Farzana Haq, Naifa Akhter, Tasnuba Karim, Laboni, Sumaya,

Tamanna for their technical direction and co-operation which enabled me to work in a very

congenial and comfortable atmosphere.

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AbstractSpondias pinnata is an evergreen parasitic plant under the family anacardiaceae and used

traditionally in medicinal system. This plant reported to have in vitro antioxidant activity

(non-enzymatic hemoglobin glycosylation), and antibacterial activity. The intention of the

study was to emphasize on the separating the compounds of methanol extracts of S.pinnata

according to their polarity and evaluate their cytotoxicity, antioxidant activity and

antimicrobial activity. To fulfill this purpose I did partition of the extracts of S.pinnata into

Ethyl acetate solvent system. Then evaluate the antioxidant activity , cytotoxic property by

using brine shrimp lethality bioassay. After collection, identification and drying the tree

sample, extraction was performed by using methanol solvent system. Then partition was

done. Then extracted components were used to evaluation.

Key Words:Spondias pinnata, Brine shrimp lethality assay, Ethyl acetate

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List of Contents

CHAPTER 1: INTRODUCTION.1-17

CHAPTER 2: LITERATURE REVIEW ....18-23

CHAPTER 3: PLANT DESCRIPTION.....24-34

CHAPTER 4: COLLENTION, DRYING AND EXTRACTION...35-38

CHAPTER 5: DESIGN OF IN-VITROTEST.......................................................39-41

CHAPTER 6: METHODS AND MATERIALS.42-66

CHAPTER 7: RESULTS AND DISCUSSIONS...67-79

Conclusion.......80

References.....81-83