ROTORS, ACCESSORIES for Hanil Centrifuges · 2019-07-08 · Technical Support : +82-2-3452-8966 / [email protected] 5/53 1. Rotors Fixed angle rotor holds tubes at an angle to

Operation Manual

ROTORS, ACCESSORIES for

Hanil Centrifuges

Date of Purchase

Where Purchased

목 차0.Safety ----------------------------------------------------------- 4

1. Rotors ---------------------------------------------------------- 51.1 Fixed Angle Rotor - 51.2 Swing-out Rotor - 51.3 Angle Rotor Structure - 61.4 Pivot Type Swing-out Rotor - 81.5 Latch Type Swing-out Rotor - 81.6 Micro Plate Swing-out Rotor - 91.7 Angle Rotor Chart (RPM and Capacity) - 101.8 Angle Rotor Specifications - 111.9 Swing-out Chart (RPM and Capacity) - 141.10 Swing-out Specifications - 15

2. Preparing and Using Angle Rotors -------------------- 162.1 Preparing the Rotor - 162.2 Using Tubes - 162.3 Installing the Rotor - 172.4 Removing the Rotor/Sample - 17

3. Preparing and Using Swing-out Rotors -------------- 183.1 Preparing the Rotor - 183.2 Installing the Rotor - 183.3 Loading the Bucket/Adaptor/Sample - 193.4 Removing the Rotor/Tubes - 19

4. Bucket and Adaptor --------------------------------------- 204.1 Adaptor for Angle Rotor - 204.2 Adaptor Types - 204.3 Using Adaptor - 214.4 Bucket -224.5 Buckets Types - 234.6 Using Bucket - 244.7 Adaptor for Swing-out Rotor - 254.8 Adaptor Types for Swing-out Rotor - 264.9 Using Adaptors for Swing-out Rotor - 27

5. Tubes, Bottles ------------------------------------------ 285.1 Recommended Tubes - 285.2 Using Tubes, Bottle - 345.3 Filling Volume - 355.4 Tube/Bottle Sterilization - 35

6. Maintenance ------------------------------------------- 366.1 Inspection - Angle Rotor - 366.2 Inspection - Swing-out Rotor - 376.3 Inspection - Bucket - 376.4 Inspection - Adaptor - 386.5 Storage - Angle Rotor - 396.6 Storage - Swing-out Rotor - 396.7 Cleaning - Rotor - 406.8 Sterilizing Rotor - 416.9 Cleaning - Bucket - 426.10 Sterilizing Bucket - 426.11 Cleaning - Adaptor - 436.12 Sterilizing Adaptor - 43

7. Troubleshooting -------------------------------------- 447.1 Rotor- 447.2 Tube- 45

Appendix 1Centrifugation Separations - 46

Appendix 2RCF calculation - 50Separation time - 50

Appendix 3chemical resistance of Plastics - 51

Appendix 4Chemical resistance of Aluminum - 52

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0. Safety

• Check the rotor, bucket, and tube are free of scratch and crack before operation.• Check the chemical resistance chart(Refer to Appendix3&4) and do not use any samples inapplicable to the tubes and accessories.• Do not use any samples that may generate volatile or explosive vapor.• Always use the same type of tubes. (weight, material,density and volume.)• Do not exceed the maximum rated speed of the rotors, buckets and tubes in use.• The maximum speed of the rotor may vary depending on the device or tube or adaptor.• Make sure that there is no foreign material in the chamber.• Be careful not to load the rotor with too much force.• Firmly tighten the rotor onto the motorshaft.• Remove the rotor from the chamber after centrifugation.• After using the rotor, remove the rotor from the chamber. Keep it upside down without the lid. At this time, be careful not to damage the rotor ID. disc• Do not put the rotor in the boiling water for sterilize. • There is a rotor ID. disc at the bottom of the rotor to identify the rotor ID. Make sure not to damage the rotor ID. disc when handling the rotor. • Only use rotors, parts, and accessories provided by Hanil Scientific Inc. Hanil Scientific Inc. is not responsible for damages of the device and accidents caused by using parts and accessories not recommended.• Inspect the rotor and accessories each time after using them. If you find any abnormality, stop using them and contact Hanil Scientific Inc.• It is your responsibility to sterilize and/or decontaminate the centrifuge and accessories before requesting service by Hanil Scientific service representative.• Maximum speeds are based on a solution density of 1.2 g/mL in all rotors

Rotors and accessories are may damaged in repeated use or careless handling. And damage may be worsened under centrifugal forces. Therefore even a small defect on the rotor or accessories may cause personal injury and instrument damage. Carefully read and understand this manual.

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1. Rotors

Fixed angle rotor holds tubes at an angle to the axis of rotation. It is designed of the aluminum material, so fixed-angle rotor can withstand much higher gravitational forces than swing-out rotor. It is useful for pelleting subcellular particles and remove particles from a suspension and discard the debris, and isopycnic banding of DNA.

Swing-out holds tubes in the buckets(or adaptors) which swing horizontally.It is useful when samples are to be resolved in density gradients. The longer path length permits better separation of individual particle types from a mixture.

1.1 Fixed Angle Rotor

1.2 Swing-out Rotor

Supernatant

Pellet

Pellet Centrifugal Field

Fixed Angle Rotor

Swing-out Rotor

Differential Centrifugation Density Gradient Centrifugation


① Lid Handle: After loading the rotor, turn the rotor handle clockwise to tighten the rotor.② Lid: Lid prevents tubes or parts from being bounced during centrifugation.③ Rotor Hole: Tube are inserted into the rotor hole④ Rotor Body: Body protects tubes and samples inside the rotor⑤ Rotor ID disc: Rotor ID disc identifies the loaded rotor ID. and Max. Speed. ⑥ Drive hole: Drive hole is where the motor shaft is connected.

①

②

⑤

⑥

③

④

1. Rotors

1.3 Angle Rotor Structure (for Supra, Combi series - High Speed)


1. Rotors

1.3 Angle Rotor Structure (for Fleta4 -Low Speed)

①

④

②

③

① Rotor bolt: : After loading a rotor turn the rotor bolt clockwise to tighten the rotor.② Rotor hole : Sleeves are inserted into the rotor hole/③ Drive hole: Drive hole is where the motor shaft is connected.④ Sleeve: Sleeve protects the tubes and sample.


① Pivot: Pivot is a hook that can hang bucket.② Rotor bolt: After loading the rotor turn the rotor bolt clockwise to tighten the rotor.③ Drive hole: Drive hole is where the motor shaft is connected.④ Rotor ID disc: Rotor ID disc identifies the loaded rotor ID. and Max. Speed.

①

①

②

②

③

③

④

1. Rotors

1.4 Pivot Type Swing-out Rotor (for Combi series)

1.5 Latch Type Swing-out Rotor (for Fleta5)

① Rotor latch: The latch engages with the buckets② Rotor bolt: After loading the rotor turn the rotor bolt clockwise to tighten the rotor.③ Drive hole: Drive hole is where the motor shaft is connected.


① ②

1. Rotors

1.6 Micro Plate Swing-out Rotor (for Fleta5)

① Plate bucket: Plate bucket holds plate.② Rotor bolt: After loading the rotor, turn the rotor bolt clockwise to tighten the rotor.③ Drive hole: Drive hole is where the motor shaft is connected.

③

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1. Rotors

1.7 Angle Rotor Chart (RPM and Capacity)

Roto

r tot

al ca

paci

ty

Speed/Max. RPM

6,000

4,000

3,000

1,500

500

300

180

120

100

75

50

10

10K 15K 20K 25K 30K 40K 45K 50K

A0.2-48

A250-6

A500-6

A1000-4

A1000-6

A2.0-24

A2.0U-24

A10U-12

A13.5U-8

A13.5U-12

A38.5U-6

A94U-6

A2.0-36

A10-12

A15c-12AL15-12

AL15-24 A50c-6

A85-6

A250U-6

A50-6

A50-8/A50U-8

A15-12

mL

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1. Rotors

1.8 Angle Rotor Specifications

- For Ultra 5.0 -

A13.5U-8

A13.5U-12

A38.5U-6

Rotor Shape

Cat. No. A2.0U-24 A10U-12 A13.5U-8 A13.5U-12 A38.5U-6 A50U-8 A94U-6 A250U-6

Hole angle ∠40° ∠26° ∠26° ∠26° ∠29° ∠30° ∠25° ∠25°

Max. Capacity 24 x 1.5/2.0 mL 12 x 10 mL 8 x 13.5 mL 12 x 13.5 mL 6 x 38.5 mL 8 x 50 mL 6 x 94 mL 6 x 250 mL

Size (Ø x H, mm) 180 x 88 180 x 90 165.5 x 91.3 189 x 90.5 198 x 112 213 x 110 219.7 x 119 280 x 130 Rotor hole bore Ø x L (mm) 11 x 37.5 mm 17 x 70 mm 16.3 X 83 mm 16.3 X 83.4 25.6 x 92 29 x 100.5 38.5 x 105 60 x 101

Radius* (mm) 82 85 73 87 91 98 99.4 129

Max. height for tube fit (mm) 47.1 71.5 84.4 83.4 mm 98.9 mm 107.3 mm 110.7 mm 126.9 mm

Rotor Hole Bottom type Conical Round Round Round Round Round Round Flat

Tube capacityBottom type

1.5/2.0 mLConical

10 mLRound

13.5 mLRound

13.5 mLRound

38.5 mLRound

50 mLRound

94 mLRound

250 mLFlat

K-factor 71 102 113 81 127 193 215 540

Ultra 5.0 Max. RPM(rpm)Max. RCF(xg)

40,000146,680

45,000192,430

50,000204,030

50,000243,160

50,000254,340

30,00098,784

35,000136,377

20,00057,792

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- * Mark: Adaptors available. - Maximum speed varies when using adapter.

1. Rotors


-For Supra/Combi Series-

Rotor Shape

Cat. No. A0.2-48 A2.0-24* A2.0-36* A10-12 A15S-12 A15c-12 A50-6* A50c-6

Hole angle ∠45° ∠40° ∠30° ∠36° ∠25° ∠25° ∠30° ∠23°

Max. Capacity 48 x 0.2 mL 24 x 1.5/2.0 mL 36 x 1.5/2.0mL 12 x 10mL 12 x 15 mL 12 x 15 mL conical 6 x 50 mL 6 x 50 mL

conicalSize (Ø x H, mm) 200 x 56 202 x 79 240 x 64 179.9 x 82 205 x 109 215 x 121 200 x 109 205.7 x 119Rotor hole bore Ø x L (mm) 6.5 x 17 11.5 x 38 11 x 37.5 mm 16.3 x 74.5 19 x 96 17 x 115 29 x 100 30 x 108.6

Radius (mm) 88.9 85 115.7 85.1 96.1 99.4 96.1 91

Max. height for tube fit (mm) - 46 49.5 87.1 120.8 123.2 122.7 110.7

Rotor Hole Bottom type Conical Conical Conical Round Round Conical Round Conical

K-factor 304 535 206 279 704 367 986

Supra R30

Max. RPM(rpm)Max. RCF(xg)

- - 20,00051,741

30,00085,628

30,00096,696

17,00032,116

30,00096,696

17,00029,402

Supra R22 - - 18,00041,910

22,00046,049

22,00052,001

17,00032,116

22,00052,001

17,00029,402

Supra R17 - - 15,00029,104

17,00027,496

17,00031,050

15,00025,004

17,00031,050

15,00022,891

Supra R12 - - - - - - - -

Combi R515 12,50015,530

15,00020,325

14,00025,353

15,00021,407

15,00026,664

15,00025,004

15,00024,174

15,00022,891

Combi 508 8,0006,361

8,0005,781

8,0008,279

8,0006,089

8,0007,585

8,0007,105

8,0006,876

8,0006,511

-For Fleta4-

Rotor Shape

Cat. No. AL15-12 AL15-24

Max. Capacity 12 x 15 mL 24 x 15 mLSize (Ø x H, mm) 164.6 x 42.8 180 x 90 Rotor hole bore Ø x L (mm) 18 x 104.5 17 x 70 mm

Radius (mm) 133.9 147.3

Max. height for tube fit (mm) 143.3 71.5

Fleta4 Max. RPM(rpm)Max. RCF(xg)

4,0002,635

4,0002,395


- * Mark: Adaptors available. - Maximum speed varies when using adapter.

1. Rotors


Rotor Shape

Cat. No. A50-8* A85-6* A250-6 A500-6 A1000-4 A1000-6

Hole angle ∠30° ∠25° ∠25° ∠25° ∠25° ∠25°Max. Capacity 8 x 50 mL 6 x 85 mL 6 x 250 mL 6 x 500 mL 4 x 1,000 mL 6 x 1,000 mLSize (Ø x H, mm) 213 x 110.7 209.1 x 112 295.6 x 147.8 336 x 179 350 x 196 408 x 188Rotor hole bore Ø x L (mm) 29 x 100 38.3 x 95 62 x 103 70 x 141 98 x 144 98 x 144

Radius (mm) 98.9 97.6 138.3 158.7 169.9 200.1

Max. height for tube fit (mm) 110.7 115.4 132 168.5 184.8 176.5

Rotor Hole Bottom type Round Round Flat Flat Flat Flat

K-factor 345 491 3,079 5,504 9,804 6,629

Supra R30

Max. RPM(rpm)Max. RCF(xg)

30,00099,513

25,00068,198

10,00015,462

8,00011,355

7,0009,307 -

Supra R22 20,00044,228

20,00043,647

12,00022,265

9,00014,372

8,00012,157 -

Supra R17 15,00024,878

15,00024,551

10,00015,462 - - -

Supra R12 - - 12,00022,265

10,00017,743

8,00012,157

7,00010,962

Combi R515 15,00024,878

15,00024,551

8,0009,896 - - -

Combi 508 8,0007,077

8,0006,983 - - - -

-For Supra/Combi Series-

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1. Rotors

1.9 Swing-out Chart (RPM and Capacity)

6,000

5,000

4,000

3,000

2,000

500

400

200

SPEED /RPM2K 4K 4.5K 5K

S750-4

WS750-6

.........

WS1000-6WS2400-6

HSM-2

S500-4

HSR-4S

S50-4

S85-6

Roto

r tot

al ca

paci

ty


1. Rotors

1.10 Swing-out Specifications

※ Maximum speed varies depending on the bukcets or adpators. Refer to the operating manual or website(www.ihanil.com) for more information.

Rotor Shape

Cat. No. HSR-4S S50-6 S85-4 HSM-2

Swing angle ∠90° ∠90° ∠90° ∠90°

Max. Capacity 2 x 50 mL 6 x 50 mL 4 x 85mL2(MTP/ CellCulture/DWP)

Size (Ø x H, mm)* 262 x 55 230 x 36 230 x 36 174 x 212 x 20(w x d x h)

Radius* (mm) 181.3 172.6 181.3 139

Fleta 5Max. RPMMax. RCF

5,5006,230 - - 2,000

622

Fleta 4 - 4,0003,243

4,0003,087 -

Rotor Shape

Cat. No. WS750-6 WS1000-6 WS2400-6

Swing angle ∠90° ∠90° ∠90°Max. Capacity 6 x750 mL 6 x 1,000mL 12 x 500mL blood bagSize (Ø x H, mm)* 262 x 65 384 x 99 494 x 118

Radius* (mm) 225 259.8 286.7

Component R12

Max. RPMMax. RCF

- - 4,5006,491

Component R6 - 5,0007,261 -

Component wR 4,5005,096 - -

Rotor Shape

Cat. No. S500-4 S750-4

Swing angle ∠90° ∠90°Max. Capacity 4 x 500 mL 4 x 750 mLSize (Ø x H, mm)* 262 x 55 277 x 55Radius* (mm) 194 196.5

Combi R515Max. RPMMax. RCF

4,5004,392 4,000

3,515Combi 508 4,000

3,435

-Rotors of Combi Series -

-Rotors of Fleta Series -

-Rotors of Component Series -

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2. Preparing and Using Angle Rotors

2.1 Preparing the Rotor

2.2 Using Tubes

1. Check the rotor,tube, adapter for any cracks, foreign objects and pitting. [Refer to 6. Maintenance].2. Be sure the motor shaft is not damaged.3. Check the level using a leveler tool after installing the rotor.4. Check the tubes you want to use are listed on the [5.1 Recommended Tubes].5. Refer to [Appendix 3.Chemical resistance of Plastics] before loading sample.

- Load tubes symmetrically with identical volume.- Always use the same type of tubes. (weight, material,density and volume.)- Insert sets of two tubes in opposite holes. (Refer to the figure below when loaing three tubes.)- Opposing tubes must be filled to the same volume with sample of the same density. - For more information [Refer to 5.3 Using Tubes/Bottles ].

[Correct arrangement]


2. Preparing and Using Angle Rotors

2.3 Installing the Rotor

1. Check the motor shaft and chamber are free of foreign objects.2. Gently install the rotor onto the motor shaft.3. Place the rotor lid on the rotor body and turn the rotor handle clockwise until it secured.4. Make sure the device is balanced using a leveler tool.5. For Operation, refer to the operating manual of the device.

Insufficient installing of the rotor or rotor lid may cause coming off the rotorlid and damage the device.

2.4 Removing the Rotor/Sample1. Turn the rotor handle counterclockwise and remove it.2. Carefully remove the tubes from the rotor.3. Carefully remove the rotor from the chamber.

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3. Preparing and Using Swing-out Rotors

3.1 Preparing the Rotor

3.2 Installing the Rotor

1. Check the motor shaft and chamber are free of foreign objects.2. Gently install the rotor onto the motor shaft.3. Insert the rotor bolt into the center hole of the rotor.4. Turn the rotor bolt clockwise using the wrench.5. Make sure the device is balanced using a leveler tool.6. For Operation, refer to the operating manual of the device.

1. Check the rotor,tube, bucket and adaptor for any cracks, foreign objects and pitting. [Refer to 6. Maintenance].2. Be sure the motor shaft is not damaged.3. Check the level using a leveler tool after installing the rotor.4. Check the tubes you want to use are listed on the [5.1 Recommended Tubes].5. Refer to [Appendix 3&4] before loading sample.


3. Preparing and Using Swing-out Rotors

3.4 Removing Rotor/Tubes

1. Take buckets out.2. Remove adaptors and tubes from the bucket.3. Turn the rotor bolt counterclockwise.4. Remove the rotor vertically from the chamber gently.

3.3 Loading the Bucket/Adaptor/Sample

1. Load the buckets on all pivots. ▶ For unused pivots, load the empty buckets.2. Swing the buckets by hand to ensure it moves smoothly.

3. Use the same type of tubes. (weight, material,density and volume.)4. When loading the tubes into the adaptors, be sure symmetrical loading.

[Correct tube arrangement]

[Correct bucket arrangement]

1. 2.

1. Opposing buckets must be same type. (Circle: Round buckets / Rectangle: Microplates)

2. Buckets must be loaded symmetrically.

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4. Bucket and Adaptor

4.1 Adaptor for Angle Rotor

4.2 Adaptor Types for Angle Rotors

Use only adaptors that provided by Hanil Scientific Inc.

Bore(∅)

height

Rotor Cat. No. Max. Capacity Adaptor Cat. No.

AdaptorTube Capacity

AdaptorBottom Shape

Adaptor Hole Dimension(∅ x h,mm)

Adaptor Material

A2.0-24A2.0-36

24 x 2.0 mL36 x 2.0 mL

TR0.5 0.5 mL Flat 8 x 37Acetal

TR0.2 0.2 mL Flat 6 x 21

A50-6A50-8

6 x 50 mL8 x 50 mL

TR15(50) 15 mL Round 17 x 94

Polyamide

TR15c(50) 15 mL conical Conical 17 x 105

A85-6 6 x 85 mL

TR50(85) 50 mL Round 29 x 95

TR50c(85) 50 mL conical Conical 29.5 x 100

TR15(85) 15 mL Round 17 x 94 TR15c(85) 15 mL conical Conical 17 x 100

AL15-12AL15-24

12 x 50 mL24 x 50 mL

TR5(15) 3.5-7 mLblood tube Round 13.5 x 85 Acetal

TR3(15) 2-4.5 mLblood tube Round 13.5 x 61 Acetal

TR5c(15) 5 mL conical Conical 14 x 20 Acetal

Many rotors can accommodate various tube sizes by using adapters.



4.3 Using Adaptor

Make sure that the adaptor you are using is for a round tube or a conical tube to prevent damage of the device or tube.

Before use

After use

Using adaptor

Adaptor storage

1. Check the adaptor if there are any cracks and foreign objects.2. Check the adaptor hole is free of foreign objects or moisture.

1. Remove the adaptor from the bucket. 2. Check for adaptor for sample leaks. If necessary, clean or autoclave them.

1. Install the rotor onto the motor shaft.2. Load adaptors into the buckets.3. Load tubes into the adaptors.

1. Keep the adaptor in a dry place. 2. Keep the adaptor out of the direct rays or heater.

There is a hole on the bottom of the adaptor.

The bottom of the adaptor is blocked.(No hole)

Adaptors for Conical tubes Adaptors for Round tubes

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4.4 Bucket

Swing-out rotor holds tubes using the buckets and adaptors.The bucket swing out to a horizontal position while centrifugation.


Use only buckets that provided by Hanil Scientific Inc.


4.5 Buckets Types

Rotor S500-4 S750-4 / WS750-6 WS1000-6 WS2400-6

Bucket

B500 B750S B750 (Lid Available)

B1000(Lid Available)

B1000 B2400

BucketMax. Capacity 500 mL 750 mL 1,000 mL 2,400 mL

BucketMax. RPM 4,500rpm (S750-4)

4,000rpm(WS750-4)4,500rpm 5,000rpm 4,500rpm

Bucket Material Aluminium

Bucket bore(∅ x h, mm) 80 x 111 99 x 103 98.5 x 143 176.9 x 150.8

Rotor S85-4 S50-4 HRS-4S

Bucket

B50 B85 HBT50-1 HBT50-2 HBT50c-1 HBT15-8 HBT15c-4

BucketMax. Capacity 50 mL 85 mL 50 mL 50 mL 50mL

conical 15 mL 15 mLconical

BucketMax. RPM 4,000rpm 4,000rpm 5,500rpm 4,500rpm 5,500rpm 4,000rpm 4,000rpm

BucketMaterial Aluminium Aluminium SUS SUS SUS SUS SUS

Bucket bore(∅ x h, mm) 30.3 x 93.5 38.5 x 88.5 30 x 99 30 x 99 31.5 x 116 18 x 89.5 18 x 99.5


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Before Use

After Use

Using Bucket

Adaptor Strorage

1. Check the bucket if there are any cracks and foreign objects. Particularly frequent use makes it easy for foreign objects to stick in the bucket grooves.2. Check the bucket grooves are lubricated. 3. Swing the bucket with a hand to check for the bucket moves smoothly.

1. Remove the bucket from the rotor.2. After run, check the adaptor for sample leaks. If necessary, clean or autoclave the bucket.

1. For buckets with lid, remove the lid.2. Keep the bucket upside down on a flat place.3. Always keep the bucket empty.

1. Install the rotor onto the motor shaft.2. Hang the buckets on the rotor pivot.3. Load the adaptors and tubes.

4.6 Using Bucket



4.8 Adaptor Types for Swing-out Rotor

Use only adaptors that provided by Hanil Scientific Inc.

Bucket/ Bucket Capacity Available Adaptor

Adaptor Max.Capacity

/Bottom Type

Adaptor Bore(∅ x h,mm) Adaptor Material

B500/ 500 mL

TR500(500) 1 x 500mL / Flat 73 x 125

ABS

TR250(500) 1 x 250mL / Flat 62 x 100

TR100(500)1x 100mL(85mL)

/Round38.5 x 84.5

TR50-4 4 x 50mL / Round 29.5 x 85.5

TR50c-3 3 x 50 mL / Conical 30 x 90

TR15-9 9 x 15 mL / Round 16.5 x 85TR15c-7 7 x 15 mL / Conical 17 x 95.5TR10-9 9 x 10 mL / Round 17 x 64TR5-9 9 x 5 mL / Round 13 x 64

v/ 750 mL

TR500(750) 1 x 500 mL / Flat 75.5 x 98.7 Polypropylene

TR500c(750) 1 x 500 mL / Conical 99 x 58

Polyamide

TR250(750) 1 x 250 mL / Flat 62.3 x 87TR225c(750) 1 x 225 mL / Conical 60.5 x 124.4

TR50-7 7 x 50 mL / Round 29.2 x 97TR50c-5 5 x 50 mL / Conical 29 x 91TR15-19 19 x 15 mL / Round 17.2 x 87TR15c-14 14 x 15 mL / Conical 17 x 89

TR10-21 21 x 10 mL / Round 16 x 87

TR5-24 24 x 3 or 5 mL / Round13.2 x 60 (3 mL)13.2 x 87 (5 mL)

B1000 / 1000 mL TRB480 1 x 480mL Blood bag 65.5 x 135 ABS

B2400 / 2400 mLTRB500-2 2 x 500mL Blood bag

- ABSTRB450-2 2 x 450mL Blood bagTRB250-2 2 x 250mL Blood bag

4.7 Adaptor for Swing-out Rotor


Swing-out rotors can accommodate various tube sizes by using adaptors.

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Bucket/ Bucket Capacity Available Adaptor

Adaptor Max.Capacity

/Bottom Type

Adaptor Bore(∅ x h,mm) Adaptor Material

B80/ 80 mL

TR50(85) 1 x 50mL / Round 29 x 95

PolyamideTR50c(85) 1 x 50 mL / Conical 29.5 x 95

TR15(85) 1 x 15 mL / Round 17 x 94

TR15c(85) 1 x 15 mL / Conical 17 x 100

B50/ 50 mL

TR50c(50) 1 x 50 mL / Conical 29.5 x 17.5 MC Nylon

TR25(50) 1 x 25 mL / Round 20 x 87ABS

TR15(50) 1 x 15 mL / Round 18 x 87

4.8 Adaptor Types for Swing-out Rotor



4.9 Using Adaptors for Swing-out Rotor

Before Use

After Use

Using Adaptor

Adaptor Storage

1. Remove the adaptors from the bucket.2. After run, check the adaptor for sample leaks. If necessary, clean or autoclave the adaptor.

1. Install the Swing-out rotor onto the motor shaft.2. Hang the buckets on the rotor pivots.3. Load the adaptors and tubes.


1. Check the adaptors if there are any cracks and foreign objects.2. When using the 3-stage adaptor, assemble them to fit the bucket groove line. [Refer to 6.4 Inspection-Adaptor]2. When using the 3-stage adaptor, insert the handle to the handle groove.3. Swing the bucket with a hand to check for the bucket moves smoothly.4. Make sure that the adaptor you are using is for a round tube or a conical tube to prevent damage of the device or tube.

1. Keep the adaptor in a dry place. 2. Keep the adaptor out of the direct rays or heater.

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5. Tubes, Bottles

5.1 Recommended Tubes (For Supra/Combi Series)

Rotor Adaptor Bore (Ø x L, mm) Tube Capacity Tube Tube

Material Max. g-force(xg) Tube dimension O.D. x h, mm

A10-12 - 16.3 x 74.5 10 mL Nalgene 3119-0010 PPCO 50,000 xg 16.1 x 80.5A15S-12 - 19 x 96 15-16 mL Nalgene 3110-0160 ① PPCO 50,000 xg 17.8 x 98.6

A15c-12- 17 x 115 15 mL conical FALCON 352096

Eppendorf 0030122151 PP 12,000 xg19,500 xg

17 x 12017 x 121

TR5c(15) 14 x 20 5 mL conical Eppendorf 0030119380Biosigma CL50.002.0100 PP 25,000 xg

21,000 xg 17 x 56

A50-6A50-8

- 29 x 100 50 mL Nalgene 3118-0050 ②Nalgene 3119-0050 ②

PCPPCO

50,000 xg100,605 xg

28.8 x 106.428.8 x 105.9

TR15(50) 17 x 94 15 mL Nalgene 3110-0150 ① PPCO 50,000 xg 15.8 x 112.8

TR15c(50) 17 x 105 15 mL conicalFALCON 352096

Eppendorf 0030122151Corning 430052

PP12,000 xg19,500 xg12,000 xg

17 x 12017 x 121

17 x 118.5A50c-6 - 30 x 108.6 50 mL conical FALCON 352070 PP 16,000 xg 30 x 115

A85-6

- 38.3 x 95 81 mL Nalgene 3118-0085 PC 50,000 xg 38.2 x 105.2

TR50(85) 29 x 95 50 mL Nalgene 3118-0050 ② 3119-0050 ②

PCPPCO

50,000 xg100,605 xg

28.8 x 106.4 28.8 x 105.9

TR50c(85) 29.5 x 100 50 mL conicalOrange 4440100NFALCON 352070

SPL 50050

PPPPPP

12,000 xg16,000 xg14,000 xg

30 x 11430 x 11530 x 115

TR15(85) 17 x 94 15 mL Nalgene 3110-0150 ① PPCO 50,000 xg 15.8 x 112.8TR15c(85) 17 x 100 15 mL conical FALCON 352096 PP 12,000 xg 17 x 120

A250-6 - 62 x 103

250 mL Nalgene 3120-0250 ③ PPCO 13,200 xg 61.8 x 125.2250 mL Nalgene 3122-0250 ③ PC 27,500 xg 61.8 x 125.2

250 mL Autofil 1801-RLS ④ Autofil 1803-RLS ⑤ PP 8,250 xg

27,500 xg 61 x 127

250 mL Autofil 1802-RLS ④Autofil 1804-RLS ⑤ PC 8,250 xg

31,700 xg 61 x 127

A500-6 - 70 x 141

500 mL Autofil 1805-RLS ④ Autofil 1807-RLS ⑤ PP 8,250 xg

13,500 xg 69 x 165

500 mL Autofil 1806-RLS ④ Autofil 1808-RLS ⑤ PC 8,250 xg

13,850 xg 69 x 165

500 mL Nalgene 3120-9500 3122-0500

PPCOPC 13,700 xg 69.5 x 158.9

500 mL Hanil HI_TB-500 PP 14,300 xg 68.5 x 163 A1000-4A1000-6 - 98 x 144 1,000 mL Nalgene 3120-1000 ⑥ PPCO 7,100 xg 97.5 x 174.2

① Use with friction fit closures (Cat. No. DS3111-series) for high speed centrifugation.② For leakproof performance >10,000 xg, use a sealing closure DS3131-0024.③ To assure leakproof, especially at higher speeds and fill volumes, use sealing closure DS3131-0038.④ Bottle with screw cap for low speeds. ⑤ Bottle with leak-proof sealing cap for high speeds.⑥ To assure leakproof, especially at higher speeds and fill volumes, use sealing closureDS3132-0063


5. Tubes, Bottles

5.1 Recommended Tubes (For Fleta 4)

Rotor Sleeve Adaptor Bore (Ø x L, mm)

Tube Capacity Tube Tube


AL15-24AL15-12 TR15(15)

- 18 x 94.5 15 mL Nalgene 3110-0150 ① PPCO 50,000 xg 17.8 x 98.6

- 18 x 94.5 15 mL conical

FALCON 352096Eppendorf 0030122151

Corning 430052PP

12,000 xg19,500 xg12,000 xg

17 x 12017 x 121

17 x 118.5

TR5c(15) 14 x 20 5 mL conical

Eppendorf 0030119380Biosigma CL50.002.0100 PP 25,000 xg

21,000 xg 17 x 56

- 18 x 94.5 10 mLblood tube

BD VacutainerVacuette - - 16 x 100

TR5(15) 13.2 x 58 3.5-7 mLblood tube


TR3(15) 13.2 x 82 2.0-4.5 mLblood tube


▶ Microtubes are excluded.▶ Recommended microplates information is currently being updated.▶ These tube specifications above are provided by tube manufacturers.

Tube MaterialPPCO : Polypropylene copolymerPP : PolypropylenePC : Polycarbonate

① Use with friction fit closures (Cat. No. DS3111-series) for high speed centrifugation.

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5. Tubes, Bottles

5.1 Recommended Tubes (For Ultra 5.0)

Rotor Hole Bore (Ø x L, mm)

Tube Capacity Tube Tube Material Max. g-force(xg) Tube dimension

O.D. x h, mm

A2.0U-24 11 x 37.5 1.3 mL Hitachi 84710901 Polyallomer 145,966 xg 11 x 37.5

A10U-12 16.3 x 70 8 mL / 10 mL Beckman 344621 / 344622 Polyallomer 402,000 xg 16 x 67

A13.5U-8 16.3 x 83 9 mL Hitachi S303922A Polycarbonate 390,000 xg 16 x 82

A13.5U-12 16.3 x 83.4 8mL Hitachi 325952A Polycarbonate 260,000 xg 16 x 82

A38.5U-6 26 x 9235.5 mL Beckman 363073 Polypropylene 504,000 xg 25.3 x 92

26 mL Hitachi S308132A / S303926A Polyallomer 303,000 xg 26 x 97

A50U-8 29 x 100.5 50 mL Beckman 357001 Stainless Steel 235,000 xg 29 x 100.5

A94U-6 38.3 x 105 94 mL Beckman 303380 / 345775 / 345777

Stainless Steel / Polypropylene /Ultra-Clear™"

235,000 xg 38 x 102

A250U-6 62 x 101 250 mLBeckman 334205 / 355627 Polypropylene 53,900 xg 60 x 120

Nalgene 3120-0250 3122-0250 Polypropylene 13,200 xg /

27,000 xg 61.8 x 125.2



5.1 Recommended Tubes(For Swing-out Rotors)

Bucket Adaptor Bore (Ø x L, mm)



B750B750S

- 99 x 103(B750) 750 mL Thermo 75003699 PP - 98 x 133TR500(750) 75.5 x 98.7 500 mL Hanil HI_TB-w500 PP 4,300 xg 73.7 x 143TR500c(750) 95.6 x 58 500 mL Corning 431123 PP 6,000 xg 95.5 x 148.6

TR250(750) 62.3 x 87 250 mL

Nalgene 3120-0250 ①Nalgene 3122-0250 ①

Autofil 1801-RLS ② Autofil1803-RLS ③Autofil 1802-RLS ② Autofil 1804-RLS ③

PPCOPCPPPPPCPC

13,200 xg27,500xg8,250xg27,500xg8,250xg31,700xg

61.8 x 125.261.8 x 125.2

61 x 12761 x 12761 x 12761 x 127

TR225c(750) 60.5 x 124.4 225 mLconical Falcon 352075 PP 16,000 xg 30 x 115

TR50-7 29.2 x 97 50 mL Nalgene 3118-0050 ④Nalgene 3119-0050 ④

PCPPCO

50,000xg100,605xg

28.8 x 106.4 28.8 x 105.9

TR50c-5 29 x 91 50 mL conical

Orange 4440100NFalcon 352070

Eppendorf 0030122178PP

12,000 xg16,000 xg19,500 xg

30 x 11430 x 115

30 x 116.2 TR15-19 17.2 x 87 15 mL Nalgene 3110-0150 ⑤ PPCO 50,000 xg 15.8 x 112.8

T15c-14 17 x 89 15 mL conical


Corning 430052PP

12,000 xg19,500 xg12,000 xg

17 x 12017 x 121

17.5 x 118.5

T10-21 16 x 8710 mL BD vacutainer - - 16 x 1009 mL Vacuette - - 16 x 100

T5-24

13.2 x 58 (3 mL)

2 ~ 4.5 mL2 ~ 4 mL


13.2 x 85 (5 mL)

3.5 ~ 7 mL5 ~ 6 mL


S750-4 / WS750-6

5. Tubes, Bottles



① To assure leakproof, especially at higher speeds and fill volumes, use sealing closure DS3131-0038.② Bottle with screw cap for low speeds. ③ Bottle with leak-proof sealing cap for high speeds.④ For leakproof performance >10,000 xg, use a sealing closure DS3131-0024.⑤ Use with friction fit closures (Cat. No. DS3111-series) for high speed centrifugation.

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5.1 Recommended Tubes (For Swing-out Rotors)

S500-4

Bucket Adaptor Bore (Ø x L, mm) Tube Capacity Tube Tube Material Max. g-force(xg) Tube dimension

O.D. x h, mm

B500

TR500(500) 73 x 125 500 mL Hanil HI_TB-w500 PP 4,300 xg 73.7 x 143

TR250(500) 62 x 100 250 mL

Nalgene 3120-0250 ①Nalgene 3122-0250 ①Autofil 1801-RLS ② Autofil1803-RLS ③ Autofil 1802-RLS ②Autofil 1804-RLS ③

PPCOPCPPPPPCPC

13,200 xg27,500xg8,250xg27,500xg8,250xg31,700xg

61.8 x 125.261.8 x 125.2

61 x 12761 x 12761 x 12761 x 127

TR100(500) 38.5 x 84.5 81 mL Nalgene 3118-0085 PC 50,000 xg 38.2 x 105.2

TR50-4 29.5 x 85.5 50 mL Nalgene 3118-0050 ④Nalgene 3119-0050 ④

PCPPCO

50,000xg100,605xg

28.8 x 106.428.8 x 105.9

TR50c-3 30 x 90 50 mL conical



12,000 xg16,000 xg19,500 xg

30 x 11430 x 115

30 x 116.2 TR15-19 16.5 x 85 15 mL Nalgene 3110-0150 ⑤ PPCO 50,000 xg 15.8 x 112.8

T15c-7 17 x 95.5 15 mL conicalFALCON 352096

Eppendorf 0030122151Corning 430052

PP12,000 xg19,500 xg12,000 xg

17 x 12017 x 121

17.5 x 118.5

T10-9 17 x 6410 mL BD vacutainer - - 16 x 1009 mL Vacuette - - 16 x 100

TR5-9 13 x 64

2 ~ 4.5 mL BD Vacutainer - - 13 x 752 ~ 4 mL Vacuette - - 13 x 75

3.5 ~ 7 mL BD Vacutainer - - 13 x 1005 ~ 6 mL Vacuette - - 13 x 100

5. Tubes, Bottles

S50-6Bucket Adaptor Bore

(Ø x L, mm) Tube Capacity Tube Tube Material Max. g-force(xg) Tube dimension O.D. x h, mm

B50

- 30.3 x 93.5(Bucket) 50 mL Nalgene 3118-0050

Nalgene 3119-0050PC

PPCO50,000 xg100,605 xg

28.8 x 106.4 28.8 x 105.9

TR50c(50) 29.5 x 17.5 50 mL conical



12,000 xg16,000 xg19,500 xg

30 x 11430 x 115

30 x 116.2

TR15(50) 17 x 94 15 mL Nalgene 3110-0150 ⑤ PPCO 50,000 xg 17 x 120

- 17 x 86.5(Bucket) 15 mL conical


Corning 430052PP

12,000 xg19,500 xg12,000 xg

17 x 12017 x 121

17.5 x 118.5

① To assure leakproof, especially at higher speeds and fill volumes, use sealing closure DS3131-0038.② For leakproof performance >10,000 xg, use a sealing closure DS3131-0024.③ To assure leakproof, especially at higher speeds and fill volumes, use sealing closure DS3131-0038.④ For leakproof performance >10,000 xg, use a sealing closure DS3131-0024.⑤ Use with friction fit closures (Cat. No. DS3111-series) for high speed centrifugation.



S85-4

HSR-4S

Bucket Adaptor Adaptor Bore (Ø x L, mm)



B85

- 38.5 x 88.5(Bucket) 81 mL Nalgene 3118-0085 PP 16,000 xg 28.8 x 106.4

TR50(85) 29 x 95 50 mL Nalgene 3118-0050 ① PC 50,000 xg 28.8 x 106.4

TR50c(85) 29.5 x 100 50 mL conical



12,000 xg16,000 xg19,500 xg

30 x 11430 x 115

30 x 116.2 TR15(85) 17 x 94 15 mL Nalgene 3110-0150 ② PPCO 50,000 xg 15.8 x 112.8



Corning 430052PP

12,000 xg19,500 xg12,000 xg

17 x 12017 x 121

17.5 x 118.5

Bucket Adaptor Bore (Ø x L, mm)



HBT50-1 - 30 x 99 50 mL Nalgene 3118-0050 ① PC 50,000 xg 28.8 x 106.4

HBT50-2 - 30 x 99 50 mL Nalgene 3118-0050 ① PC 50,000 xg 28.8 x 106.4

HBT50c-1 - 31.5 x 115.9 50 mL conical



12,000 xg16,000 xg19,500 xg

30 x 11430 x 115

30 x 116.2

HBT15-8

- 18 x 89.5(Bucket) 15 mL Nalgene 3110-0150 ② PPCO 50,000 xg 15.8 x 112.8

- 18 x 89.5(Bucket)

10 mL9 mL

BD vacutainerVacuette - - 16 x 100

TR3(15) 13.5 x 61 2 ~ 4.5 mL2 ~ 4 mL


TR5(15) 13.5 x 85 3.5 ~ 7 mL5 ~ 6 mL



Eppendorf 0030119380Biosigma CL50.002.0100 PP 25,000 xg

21,000 xg 17 x 56

5.1 Recommended Tubes(For Swing-out Rotors)

5. Tubes, Bottles


① For leakproof performance >10,000 xg, use a sealing closure DS3131-0024.② Use with friction fit closures (Cat. No. DS3111-series) for high speed centrifugation.

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5.2 Using Tubes, Bottles

Correct arrangement

For safety, balance the tubes simply by leveling the sample.

Balancing

5. Tubes, Bottles

- Load tubes symmetrically with identical volume.- Always use the same type of tubes. (weight, material,density and volume.)- Insert sets of two tubes in opposite holes.- Opposing tubes must be filled to the same volume with sample of the same density.

Correct arrangement for Swing-out rotor Wrong arrangement for Swing-out rotor


5.3 Filling Volume

5.4 Tube/Bottle Sterilization

It is generally recommended to fill more than 80% of the a tube or bottle.However, there is an exception that must be filled to the end of the container to prevent damage.Thus the minimum and maximum filling volumes should be different in accordance with the manufacturer's guidelines.

There is a risk of tube breakage when filling volume of sample is too less compared to the tubecapacity.In this case, use a tube and adapter that meet the sample volume.

Observe manufacturer’s sterilization guidelines.

Wrong use of tubes

Different Caps Different DensityDifferent Volume Different Tubes

5. Tubes, Bottles

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6. Maintenance

6.1 Inspection - Angle Rotor

(*1) After run, ensure that the sample was not leaked to the rotor hole. If neseccary, clean or autoclave the rotor .

(*2) If the rotor ID disc is dirty or detached , rotor id is not identified.

Check the rotor as follows after or before every use.

1. Make sure that the rotor threads are free of wear .

3. Make sure that there is no cracks or other signs of damage on the rotor body.

5. Make sure that there is no foreign objects on the rotor ID disc.(*2)

2. Make sure that there is no foreign objects in the rotor holes.(*1)

4. Make sure that there is no foreign objects on the drive hole.

6. Make sure that there is no foreign objects on the motor shaft.

Rotors and accessories are may damaged in repeated use or careless handling. And damage may be worsened under centrifugal forces.

② ③

④

⑥

①

⑤


5. Make sure that there is no foreign objects on the rotor ID disc.(*2)

2. Make sure that there is no foreign objects in the rotor holes.(*1)


6. Make sure that there is no foreign objects on the motor shaft.

6.3 Inspection - Bucket

1. Make sure that there is no foreign objects in the bucket.

3. Make sure that the bucket grooves are clean. Regularly lubricate the bucket grooves.

5. Make sure that there is no foreign objects on the rotor ID. disc.

1. Make sure that there is no foreign objects on the rotor pivots.

2. Make sure that there is no foreign objects on the bucket threads.(B750S)


4. Make sure that there is no cracks or other signs of damage on the bucket.

①

①

②

②

③

③

④

④⑤

6. Maintenance

6.2 Inspection - Swing-out Rotor

2. Make sure that the threads are free of wear .

3. Make sure that there is no cracks or other signs of damage on the rotor.

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1. Make sure that there is no foreign objects in the adaptor.

6.4 Inspection - Adaptor

1. Make sure that there is no foreign objects in the adaptor.

3. Make sure that the adaptor you are using is for a round tube or a conical tube to prevent misuse.

3.When using the 3-stage adaptor, insert the handle to the handle groove.

4. When using the 3-stage adaptor, assemble them to fit the bucket groove line.

2. Make sure that there is no cracks or other signs of damage on the adaptor.

①

①

②

②

③

③

④

6. Maintenance

2. Make sure that there is no cracks or other signs of damage on the adaptor.


6.5 Storage - Angle Rotor

6.6 Storage - Swing-out Rotor

1. After centrifugation, remove the rotor from the chamber.2. Remove the lid and keep it upside down. 3. Be careful not to damage the rotor ID disc. 4. Spread the grease lightly on the rotor thread after cleaning or periodically.

1. After using the rotor, remove the rotor from the chamber.2. Keep the rotor on a flat surface.3. Be careful not to damage the rotor ID disc. 4. Spread the grease lightly on the rotor pivot after cleaning or periodically.

6. Maintenance

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6.7 Cleaning - Rotor

Clean the rotor immediately if salts or other corrosive materials are leaked.

When foreign objects adheres to the rotor

1. Wash the rotor with a mild detergent(pH 5-9), using a soft brush.2. Rinse thoroughly with distilled water. 3. Wipe off the moisture with soft cloth and dry the rotor upside down

Soak the rotor in warm water for a while, then gently remove foreign objects with a soft brush.

[Angle Rotor]After dry the rotor, spread the grease lightly on the rotor threads.[Swing-out Rotor]After dry the rotor, spread the grease lightly on the rotor pivot.

- Do not use sharp tools on the rotor, as the surface can get scratched.- Do not use any aggressive chemicals on the rotor, such as acetone, strong acids/base, or formaldehyde etc.

6. Maintenance



- Do not use sharp tools on the rotor, as the surface can get scratched.- Do not use any aggressive chemicals on the rotor, such as acetone, strong acids/base, or formaldehyde etc.

6.8 Sterilizing Rotor

- Remove the lid and place the rotor upside down in an autoclave.- Autoclave at 121°C for 20 minutes.- Remove any contaminants with a cloth soaked in ethanol(70%).

- Check the proper time and temperature when autoclaving.- Do not put the rotor in boiling water.

- Ethanol is a flammable substance.- Do not use ethanol near the centrifuge.

6. Maintenance

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6.9 Cleaning - Bucket

6.10 Sterilizing Bucket

6. Maintenance


1. Wash the bucket with a mild detergent(pH 5-9), using a soft brush.2. Rinse thoroughly with distilled water. 3. Wipe off the moisture with soft cloth and dry the bucket upside down

- Do not use sharp tools on the bucket, as the surface can get scratched.- Do not use any aggressive chemicals on the bucket, such as acetone, strong acids/base, or formaldehyde etc.

- Autoclave at 121°C for 20 minutes.

- Observe time and temperature when using an autoclave.- Do not autoclave the bucket lid.

- Autoclave at 121°C for 20 minutes.



- Do not use sharp tools on the bucket, as the surface can get scratched.- Do not use any aggressive chemicals on the bucket, such as acetone, strong acids/base, or formaldehyde etc.

6.11 Cleaning - Adaptor

6.12 Sterilizing Adaptor

1. Wash the adaptor with a mild detergent (pH 5-9) using a soft brush.2. Do not use sharp tools on the adaptor, as the surface can get scratched.3. Rinse thoroughly with distilled water. 4. Wipe off the moisture with soft cloth.

[Autoclavable Adaptors]

※ Autoclave at 121°C for 15 minutes.

- For Angle rotor - TR0.2, TR0.5, TR15(50), TR15c(50)TR15(85), TR15c(85), TR50(85), TR50c(85)

- For Swing-out rotor - TR5-24, TR10-21, TR15c-14, TR15-19, TR50c-5, TR50-7, TR250c(750), TR250(750), TR500c(750)

Clean the adaptor immediately if salts or other corrosive materials are leaked.

6. Maintenance

- Observe time and temperature when using an autoclave.- Do not autoclave the bucket lid.

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7. Troubleshooting

7.1 Rotor

Problem Solution

Angle rotor can not be removed from the motor shaft.

[New type rotors] Contact Hanil Scientific Inc sevice team.[Old type rotors] After unscrewing the rotor bolt completely, turn the bolt clockwise 2 turns with a T-wrench.Place the lid on the rotor body and turn the lid handle with a strong force in the clockwise direction.Rotor will be released with the ping sound.

Lid can not be tightened - Check that the rotor threads are greased.- Check the rotor threads and motor shaft if there are foreign matters.

Rotor is discolored - When cleaning the rotor, it may appear discolored if not rinsed thoroughly. Rinse the rotor thoroughly with water.- Anodizing may be worn. Contact Hanil Scientific Inc sevice team.

Rotor ID. is not identified Make sure the rotor ID. disc is attached to the bottom of the rotor.

Imbalance error- Rotor imbalance may cause imbalance error. To balance the rotor, fill tubes with sample of the same density and volume. And load tubes symmetrically.


7. Troubleshooting

7.2 Tube

Problem Solution

Sample leaks out- Close the tube cap tightly.- Tube volume exceeds maximum filling volume. Refer to the tube manufacturer’s guideline for tube volumes

Tube is cracked

- Tubes may crack if they are used below their lower temperature limit. or higher g-force. Refer to the tube manufacturer’s guideline for temperature limit and Max. g-force.- Repeated use of tube may cause cracks. Dispose of cracked tubes

Tube is broken

- Sample volume is too low to support tube wall. Refer to the tube manufacturer’s guideline for tube volumes.- Tubes run above their rated speed. Refer to the tube manufacturer’s guideline for maximum speed of tubes.

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Appendix 1.

Pelleting, Differential Centrifugation

Differential Centrifugation

Centrifugation Separations

The most common centrifugation method is pelleting.Pelleting separates particles of different sedimentation coefficients.During centrifugation, the largest particles in the sample traveling to the bottom of the tube first.Differential centrifugation is the successive pelleting of particles from previous supernatant of decreasing sedimentation velocities, using increasingly higher forces and/or long run times.Through centrifugation two fractions are gained. A pellet and a supernatant of unsedimented material.The supernatant can be re-centrifuged at higher speeds to get a new pellet and supernatant.

Centrifugation Time

Cent

rifug

al F

orce

A. B. C. D.


Appendix 1.

Nucleic Acids

Yeast tRNA

1

2

3

4

6

7

8

9

20

40

60

80

200

400

600

800

2000

4000

6000

8000

Viruses

Rous sarcomaFeline leukemiaBacteriophage T2

PoliomyelitisTobacco mosaicEquine encephalitis

E. coli rRNACalf liver DNAVesicular stomatitis virus RNABacteriophage T5 DNABacteriophage T2 & T4 DNAs

Broad bean mottle

SubcellularParticles

Soluble Proteins

Plasma membranes

Cytochrome c

Collagen

Albumin

Luteinizing hormone

Immunoglobulin G

Aldolase

Catalase

-Macroglobulin

5

0

10

100

1 000

10 000

100 000

Mitochondria

Microsomes

Polysomes

Ribosomes

Ribosomal subunits

Sedimentation Coefficients

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Density gradient Centrifugation

Samples are layered on top of the density gradient. Under centrifugal force, particles sediment through the gradient column in zones. Each zone consisting of size, sedimentation rate of particles.When applying the sample to the density gradient, insert it carefully so that it does not mix with the density gradient.The centrifugation should be stopped before any of the separated zones reach the bottom of the tube.

Isopycnic gradient separates particles on the basis of density rather than size, independent of time.Each particle sediments in the tube where the gradient density is equal to the particle density.Unlike Rate-zonal centrifugation method, density gradient and the sample are mixed in the tube.

Density gradient Centrifugation permits for the complete separation of several or all components in a sample. Tube is partially filled with a solution having a decreasing density from the bottom to the top of the tube. This method is mainly suited to the viruses.There are two methods in which density gradients may be used. : Rate-zonal and isopycnic.

Rate-zonal Centrifugation

Rate-zonal Centrifugation Isopycnic Centrifugation

Isopycnic Centrifugation

Sample zone

Density gradient

Cent

rifug

al F

orce

A. B. C.

Cent

rifug

al F

orce

A. B.

Appendix 1.


Density gradient medium

Appendix 1.

Gradient medium type Principle uses

Polyhydric alchols

- Sucrose Oraganelles, membrane vesicle, virus, protein, ribosome, polysome

- Glycerol Mammalian cells(infrequent), protein

- Sorbitol NonMammalian subcellular particles

- Ficoll, polysucrose and dextrans

Mammalian cells(sometimes in combination with iodinated density gradient media), mammalian subcellular particles(infrequent)

Inorganic salts

- CsCl DNA, Virus, Protein

- Cs2SO4 DNA, RNA

- KBr Plasma lipoprotein

Iodinated gradient media

- Diatrizoate Mainly as component of commercial lymphocyte isolation media

- Nycodenz Histodenz Mammalian cells, organelles, membrane, vesicle, virus

- Percoll Mammalian cells, organelles, membrane, vesicle(infrequent)

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RCF calculation

Separation time

RCF (relative centrifugal force) refers to the amount of acceleration or force exerted on the sample in a centrifuge.RCF is obtained from the below formula.

The sedimentation time t is calcurated using sedimentation codfficients s and k factor of the rotor.

rmax : Max. Rotor Radius rmin : Min. Rotor Radius K : k-factor (rotor efficancy)S : Sedimenation coefficient

Appendix 2.


Tube/Bottle Material polypropylene Polycarbonate Polypropylene

Copolymer

Acids(dilute or weak) S M S

Acids S U S

Alcohols S U S

Aldehydes M M M

Bases S U S

Esters M U M

Hydrocarbons(aliphatic) M U M

Hydrocarbons(aromatic) M U M

Hydrocarbons(halogenated) M U M

Ketones M U M

Oxidizing agents(strong) U M U

S: Satisfactory resistance M: Marginal resistance U: Unsatisfactory resistance

※ This information has been assembled from a variety of sources.Resistance may vary depending on temperature and pressure.To prevent failure, always recommend test run before use.

Appendix 3.

Chemical resistance of Plastics

Before inserting the sample into the tube, refer to below table.

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Gradient MediumCesium acetate MCesium bromide MCesium chloride MCesium formate MCesium iodide MCesium sulfate MDextran sulfate MFicoll paque MGlycerol MMetrizamide MRubidium bromide M

Rubidium chloride M

Sodium bromide USucrose MSucrose, alkaline M

Gradient MediumAcetone(50%) SAmyl alcohol SBenzene SCresol SDiethyl ether SEthyl acetate MEthyl alcohol SMethylene chloride U

S: Satisfactory resistance M: Marginal resistance U: Unsatisfactory resistance

Appendix 4.

Chemical resistance of Aluminum

Gradient MediumAcetic acid S

Acetic acid(5%) SAcetic acid(60%) S

Acetic acid(glacial) U

Aqua regia UChromic

acid(10%) U

Chromic acid(50%) U

Hydrochloric acid(10%) U

Hydrochloric acid(35%) U

Hydrofluoric acid(10%) U

Hydrofluoric acid(50%) S

Lodoacetic acid SOleic acid UPicric acid U

Sulfuric acid(10%) U



Before inserting the tubes into the rotor, refer to below table.

※ This information has been assembled from a variety of sources.Resistance may vary depending on temperature and pressure.To prevent failure, always recommend test run before use.


Before inserting the tubes into the rotor, refer to below table.

Rotor & Accessories Manual

Combi R508, Fleta4, Fleta5, Component R12, Component R6, and Component wR

© 2018 Hanil Scientific Inc.

- This contents in this manual are subject to change for improvement without notice.

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ROTORS, ACCESSORIES for Hanil Centrifuges · 2019-07-08 · Technical Support : +82-2-3452-8966 / [email protected] 5/53 1. Rotors Fixed angle rotor holds tubes at an angle to