Ronozyme p5000(Ct)

The story of an excellent productcreated in a strong Alliance

told by: Yu-Guo Sun (DSM) & Frank Hatzack (Novozymes)

Frank Hatzack – China visit 2005

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World leader in Enzymes!• R&D• Production• Granulation

World leader inAnimal Nutrition!• Nutrition Expertise• Global Distribution• Global Marketing

A strong alliance of two world leaderscreated an excellent product


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Why is so successful?

An excellent Phytase enzyme...

…in an excellentgranulate...

…with excellent bio-efficacy!

Because it is:


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An excellent Phytase enzyme


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Peniophora Phytase is a 6-Phytase

1

2

34

5 6

OPO32-

2- O3PO

2- O3PO2- O3PO

OPO32-

OPO32-

Aspergillusphytases

Peniophora phytase

Lily pollen phytase


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Peniophora Phytase is a FAST Enzyme!

Peniophore lycii, wt

0

1

2

3

4

5

6

0 5 10 15

NatuPhos

0

1

2

3

4

5

6

0 5 10 15

NMR experiment measuring the number of P-groups released per minute核磁共振试验显示每分钟释放出的磷的集团…

Aspergillus phytase

min min

P-gr

oups

rele

ased 20 FYT/ml

1 FYT/ml

20 FYT/ml

1 FYT/ml

Peniophora phytase


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Phytase pH profiles

0.0

0.3

0.5

0.8

1.0

1.3

3 4 5 6 7 8

pH

Rel

ativ

e ac

tivity

P.lycii

A.f icuum

Chineese

Solubility of phyate is zero above pH 6because of Ca-phytate precipitationpH＞6时，植酸的溶解性为零因为钙与植酸结合成植酸钙Ca-phytate，形成一种不溶性的沉淀。

The pH profile of RONOZYME P phytase植酸酶的PH值

more acidicpH optimum

than Aspergillusphytase!

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Phytate degradation in the pig植酸在猪消化道中的降解

pH 1.5 – 5.45 min – 18 hours

pH 6.5 – 7.53 – 8 hours

pH 6.5 – 7.02 – 24 hours

Phytate solubility highpH OK for phytaselong retention time •Phytate solubility low

•pH not optimal for phytase


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The digestive tract of poultry植酸酶在家禽消化道中的降解

pH 4 – 6.5up to 2 h

pH 3.5 – 4.50.5 min – 2 min

pH 2.5 – 3.51 min – 10 min

pH 5.5 – 7.51 – 4 h

Phytase pH optimumPhytate solubility high(if pH < 6) long retention timePhytate solubility high

OK for phytase•but short retention time

•Not optimal for phytase•Phytate solubility low

pH 6.5 – 7.02 – 6 h

前胃

肌胃

胰腺

泄殖腔

嗉囊


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So: Peniophora phytase in is

1. is a very fast enzyme, faster than Aspergillus

2. with a more acidic pH optimum than Aspergillus

3. these features make it highly effective in the upper part of the digestive tract


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An excellent Granulate


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Why do you need a stable phytase?

Want no losses during processingExcellent overall handling characteristics

No Dust & Physical strength

Want storage stability for long time

Storage stability even more importantEnzyme must be stable in presence ofminerals and other additives

Mash, Condensed

Premix

Want to use for pelleting at 85C Want storage stability

Pellet feed


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CT – Coated Thermostable Granulate

Multi-layer coating1. for pelleting stability2. enzyme release3. dust-free

Enzyme stabilized inmineral&carbohydratematrixfor enzyme stability

Cellulose fibers for physical stability

400-800 microns

Patented technology

Small, defined particles1. high particle number per gram2. Excellent mixability3. Excellent homogeneity in feed


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1. Thermostability,2. Enzyme Release


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CT Coating has dual function

1. To protect the enzyme during pelleting

2. To allow release of enzyme activity in stomach


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3. A dust-free granulate


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0

50

100

150

200

RonozymeP5000 CT

Phytase N Phytase P1 Phytase P2 Phytase C2 Phytase B

mg

is dust-free (< 1 mg)


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Why is dust-free important?

1. Dust means losses of product during handling

2. Dusty products do not mix well

3. Dust is a potential health hazard


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This is a high quality granulate!

10X 25X


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See the point of coating?

Spray onorganic carrier

Competitor

CT Granulates

Enzyme totallyexposed!

-> low stability!


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Dust measurement PhytaseDone with a Heubach Dust Meter

植酸酶粉尘检测的结果RONOZYME P

Phytase P1 and F

Phytase N5000 G


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4. Excellent mixability and homogenity in feed


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What is the relation between particle size, particlenumber and mixability?

What does this mean for the animal??


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Small granulate size meanshigh particle number per gram

• particle size of 400 – 800 µm

• Average particle number per gram is > 14000!

0.0

5.0

10.0

15.0

20.0

25.0

30.0

35.0

<180 180-250 250-300 300-355 355-425 425-500 500-600 600-710 710-850 > 850

particle size intervals [祄 ]

% o

f par

ticle

s


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• Phytase N has 5000 Granules/g. Dose is 100 g/ton feed:

500.000 Granules/ton feed-> 500 Granules/kg feed -> 10 Granules/20g feed

• RONOZYME P 5000 CT has 14.200 Granules /g, dose is 100 g/ton feed

1.420.000 Granules/ton feed -> 1.420 Granules/kg feed -> 28 Granules/20g feed

With RONOZYME P there is a 3 times higher probability that a

broiler chicken gets the amount of phytase it should get each day!

What does number of particles per gram meanfor mixability and for the starter chick?每克产品中的颗粒数对小鸡料意味着什么？


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So, if competitors dose at only 50 g per ton…如果有些产品每吨只有50克添加量…

…and their product is a powder-form phytase with heterogeneous particle size distribution…

…how do you think the broiler chicken is feeling?

will I get my phytase granules

today?


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0

20

40

60

80

100

120

Samples

Enz

yme

cont

ent i

n fe

ed (%

of

aver

age)

Excellent mixability in a broiler premix

肉鸡预混料中卓越的混合均匀度

Coefficient of variation = 6.9%

变异系数 = 6.9%

VFP 21033B


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0

20

40

60

80

100

120

140

Samples

Enz

yme

cont

ent i

n fe

ed (%

of

aver

age)

Excellent mixability in a broiler feed

肉鸡饲料中的优异混合均匀度


变异系数 = 9.2%

VFP 21033A


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0

20

40

60

80

100

120

Samples

Enz

yme

cont

ent i

n pr

emix

(% o

f ave

rage

Excellent mixability in a swine premix

猪的预混料中有非常好的混合均匀度


变异系数 = 5.9%

VFP 21033BFrank Hatzack – China visit 2005

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5. Excellent PelletingStability


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Luna No.: 2004-17531-01 / TKv, PEJ•3 different batches of each product•tested independently in 2-3 runs

60

65

70

75

80

85

90

95

100

RONOZYME P RONOZYME P5000

Rec

over

y [%

] 85%

CV: 8.5% CV: 3.3%

88%

Excellent pelleting stability even at 95ºC95ºC制粒时的卓越稳定性

conditioning time 30 sec


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93 89

43

15 13

95

0

20

40

60

80

100

70癈 at 80 癈 at 90 癈

Ret

entio

n, (%

mas

h)

Ronozyme P5000 K24014

trial VFP24026

Has a much higher pelleting stability than local competitor与竞争对手在制粒稳定性上的比较


conditioning time 30 sec

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Has better storage stability in pelleted feedthan other phytase products

85

67

39

81

56

21

84

50

33

77

47

21

0

20

40

60

80

100

RONOZYME P 5000(CT)

Phytase N Phytase FRec

over

y (%

of a

ctiv

ity in

initi

al p

elle

ted

feed

)

Initial pellets 1 month @ R.T. 1 month @ 35 deg. C 3 months @ R.T.

VFP 21052

30 secs conditioning


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Comparative pelleting trial from Denmark Kolding City Biotech Institute, 2005

0.0

20.0

40.0

60.0

80.0

100.0

120.0

75C 75C 85C 85C 90C 90C 95C 95C

P CT P 5000 CT Competitor 1 Competitor 2

30 sec conditioning

75C 75C 85C 85C 90C 90C 95C 95C

RONOZYME P5000RONOZYME P (old product)

Competitor 1Competitor 2


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Is the most pelletstable phytase in the world!


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6. Biological Efficacy


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1796

2107 2110

1600

1700

1800

1900

2000

2100

2200

Control RONOZYME P (CT) RONOZYME P5000(CT)

Wei

ght g

ain

(g/b

ird)

Biological efficacy in broilersGrowth Performance (d. 1 – 36)

肉鸡的生物学活性生长成绩（d. 1 – 36 ）

broiler trial (ME08/02)

Mean values in a row with different superscripts were significantly different: a-b (P < 0.05)


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1.6

1.5891.583

1.55

1.56

1.57

1.58

1.59

1.6

1.61

1.62


FCR

(g fe

ed /

g ga

in)

Biological efficacy in broilersFCR (d. 1 – 36)

肉鸡的生物学活性饲料转化率（ d. 1 – 36 ）


Mean values in a row with different superscripts were significantly different: a-b (P < 0.05)


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49.2

52.5 52.3

47

49

51

53


Bon

e A

sh (%

)Biological efficacy in broilers

Bone Ash ( % )肉鸡的生物学活性骨骼灰份( % )



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22.9

37.1

41.7

20

25

30

35

40

45


Pho

spho

rus

dige

stib

ility

(% o

f int

ake)

Biological efficacy in pigsPhosphorus digestibility ( % )

猪的生物学活性磷的消化率( % )

pig trial (1008644)


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49.5

56.5

64.1

45

50

55

60

65


Cal

cium

dig

estib

ility

(% o

f int

ake)

Biological efficacy in pigsCalcium digestibility ( % )

猪的生物学活性钙的消化率

pig trial (1008644)


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720

794789

700

720

740

760

780

800


Dai

ly m

ean

gain

(g/d

ay)

Biological efficacy in pigsGrowth performance ( d. 0 – 30) g/day

猪的生物学活性生长成绩( d. 0 – 30) g/day

pig trial (1008644)


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2.91

2.74

2.64

2.6

2.7

2.8

2.9

3


FCR

(Kg

/ Kg)

Biological efficacy in pigsFCR (d. 0 – 30 ) kg/kg

猪的生物学活性饲料转化率 (d. 0 – 30 ) kg/kg

pig trial (1008644)


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202

236

263

160

200

240

280


Bre

akin

g st

reng

th (N

)

Biological efficacy in pigsBone strength

(external metacarpal + metatarsal combined)猪的生物学活性骨骼强度（外掌骨+）

pig trial (1008644)


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Growing pigs:Ileal apparent digestibility improvements

生长猪回肠表观消化率的改善

Val

Tyr

Thr

+ 2.6%

+ 1.8%

+ 2.1%

+ 6.2%

+ 4.3%

+ 2.7%

+ 1.4%

Ser

Pro

Phe

Orn

Met

Lys

Leu

+ 3.5 %

+ 4.7%

+ 5.7%

+2.3%

+ 32%

+ 2.1%

+ 8.4%

+ 1.9%

+ 1.8%

+ 2.9%

Ile

Hyp

His

Gly

Glu

Cys

Asp

+ 2.2%

+ 4.4%

+ 3.7%

+ 1.3%

+ 30.6%

+ 41.4%

+ 38.4%

Arg

Ala

Total N

Energy

Ca

total IPP

total P*

*measured as total digestibility

RONOZYME P (CT) at 750 FYT/kg. Eight pigs at 60 days (WhitexLandracexPietrain). Ileal-rectal anastomosis. Maize/SBM/barley diet fed ad libitum. 0.47 % P in diet. 5 periods of one week, 3 adaptation days, 4 ileal content sampling days. S06-2001.


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This is why is so successful!

An excellent Phytase enzyme...

…in an excellentgranulate...

…with excellent bio-efficacy!

Because it is:


Documents

Ronozyme p5000(Ct)