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DESCRIPTION
HONEY PRESENTATION
Citation preview
• Established in 1982, Randox employs over 1000 people
globally
• ISO 13485 Accredited manufacturer
• Distribution agreements in 130 countries
• 30% gross income reinvested into R&D
• Clinical Diagnostics, Quality Assurance programmes and
Drug Residue Testing Kits
• Over 20 years experience in developing ELISA based
technology
Revolutionising healthcare through
continuously
improving diagnostic solutions”“
About Randox
About Food
Diagnostics Improving food diagnostic industry testing by reducing costs, analysis time and labour requirements while increasing productivity output
To increase consumer confidence by improving food safety
Cost effective food diagnostic Cost effective food diagnostic
testingtesting
Honey
Placements
Current
Placements 1. Famille Michaud (France)
2. Langnese (Germany)
3. Sadecki Bartnik (Poland)
4. Bartnik LLC (Ukraine)
5. Citrofrut (Mexico)
6. Little Bee Impex (India)
7. Geomiel (Argentina)
8. Capilano (Australia)
9. Ministry of Health Cape Town (South
Africa)
10. Agro East Trade (Ukraine)
Current
Placements
11 Anses EU CRL (France)
12 Medas SRO (Slovakia)
13 Istituto Zooprofilattico Brescia (Italy)
14 Grigard (Bulgaria)
15 Apis India (India)
16 Shakti Impex Honey Co. (India)
17 Pioneer Honey (India)
18 Brij Healthcare (India)
19 SC Vladovlad SRL (Moldova)
20 Biomieles (Mexico)
21 Norevo (Mexico)
22 PE Jesa (Ukraine)
23 SQTS (Switzerland)
24 Apicultura (Bulgaria)
25 Agro East Trade (Ukraine)
26 Promiel (Argentina)
27 Carmencita (Chile)
Food Screening
Solutions
Evidence Investigator and Biochip
1
2
ELISA
Principle of ELISA technology
•The antibody is attached to the surface of the microtitre plate
•Sample and conjugate (Enzyme labelled antigen) are added simultaneously
•These compete for free binding sites on the capture antibody
•The quantity of enzyme labeled antigen bound will determine the resulting colour produced
•Optical density measures the amount of light absorbed within the solution to give a concentration of residue
ELISA Kit • A single sample preparation can be
applied across various kits
• Generic antibody extends applicability
• Short incubation time – allows for fast
results
• Microtitre plates are pre-coated with
antibodies
• Excellent sensitivity and cross-reactivity
Antibiotic Validated for Honey?
Chloramphenicol Yes
Flumequine Yes
Quinolones In Development
Streptomycin Yes
Sulphamethoxazole Yes
Tetracyclines Yes
ELISA Kits for Use in Honey
Biochip Array
Technology
A Multi-Residue Testing Platform
• Simultaneous Quantitative Detection
• One single sample
• Competitive ELISA Principles
• Efficient, accurate and cost effective
Typically a series of related residues are selected & grouped in
an array format
Potential for 22 targets present on the Biochip allowing for large
scale screening
Revolutionary
Screening
Technology
The Evidence Investigator offers revolutionary screening
technology to honey laboratories:
• Quick Time Frame• Results obtained within just a few hours
• Cost Saving Element • Compared to high ELISA volumes or analytical
methods
• Laboratory Efficiency Improved• Results are multiplexed and significant time saving
benefits are gained
The Complete
Honey Screening
Package PC & Imaging
SoftwareBarcodeScanner
Thermoshaker
Biochip Holding
Rack
Investigator Imaging
Module (Bench Top System)
Additional
Support
Covered in
System Package
• Randox will provide on-site training from a HQ based
Installation Scientist (built into quotation)
• Normally this will be a 3 – 5 day programme depending
on what Arrays are required at the site
• Full 1 year warranty and 1 year 24 hour support if
required
• Online troubleshooting also available remotely
• Training provided on all aspects of sample preparation,
assaying technique and results interpretation
Sample
Preparation of
AM IA simple dilution is completed using provided aqueous buffers such as diluted wash buffer
1.Weigh out 1g of honey
2.Add 19mls of diluted wash buffer
3.Place on a roller for 10 minutes until dissolved
The sample is now ready to be applied to the Biochip
Simple Assay Protocol
Results are processed automatically using dedicated software
Competitive
Immunoassay
Format
• Conjugate – Known Analyte labelled with Horse Radish Peroxidase (HRP) Enzyme
• HRP – Reacts with Luminol and Peroxide to give off a light reaction
• Light signal inversely proportional to antigen concentration
Endpoint
measurement
• 2 chemicals – Luminol and Peroxide generate light reaction
• Light is generated by Chemiluminescence
• Charge Coupled Device Camera captures images
• Investigator quantifies images using Relative Light Units (RLU) given off by each DTR
• Correction and Reference Spots used for image validation
Validated Kits for Honey
• Antimicrobial Array I
• Antimicrobial Array II
• Antimicrobial Array III
• Antimicrobial Array III CAP Only
• Antimicrobial Array IV
Antimicrobial Array I
Antimicrobial Array II
Antimicrobial Array III
Antimicrobial Array III CAP only
Antimicrobial Array IV
New Array in
Development(May 2014)
Nitroimidazoles Array
• Dimetridazole
• Ronidazole
• 2-Hydroxymethyl-1-methyl-5-nitro-1H-imidazole (HMMNI)
• Metronidazole
• Metronidazole-OH (MNZOH)
• Ipronidazole
• Ipronidazole-OH (IPZOH)
• Tinidazole
• Propenidazole
• Ternidazole
• Carnidazole
Advantages of
Investigator• More extensive testing menu
• Each Biochip kit is suitable for multiple sample types
• Higher throughput
• Biochip Reagents ready to use and safe to dispose
• Reduced matrix effects
Advantages of
Investigator •Less operator intervention (less probability of error / false positives)
•One incubation temperature for all Antimicrobial arrays
•Investigator false positive rates are less than 5%
•Quantitative result for each individual analyte
•Improved sensitivity
Summary • Semi-automated bench-top system
• Based on ELISA technology (multiplexed) and allows simultaneous multi-analyte assessment from a single honey sample
• Supplied as the complete package
• Simple sample preparation and easy to use, with consolidated testing over four arrays
• Unrivalled dedication to validating arrays for a number of different floral types
• Dedicated focus and support to all customers
• Cheaper pricing in comparison to sending all samples for confirmation - Intertek/ QSI
Your complete screening solution