Roger Coleman

Need

http://www.alzalliance.org/images/Forcast%20of%20Alzheimer%27s%20disease%20chart.gif

Chart 1 shows statistics and future predictions of Alzheimer'sdisease

Knowledge Base

Figure 1 shows C.elegans

http://shirleywho.files.wordpress.com/2008/09/c-elegans_esa.jpg

Knowledge Base Figure 2 shows the lifecycle of c. elegans

Knowledge Base

http://www.realage.com/health_guides/Alzheimer/img%5CLivingWithAlzheimers_artv2.jpg

Picture 3 shoes a cross reference of a normal brain and a Alzheimer's infected brain.

Knowledge Base

http://64.202.120.86/upload/image/news/getting-out-of-the-loop-of-alzheimer-s-disease/amyloid-protein-loop.jpg

Figure 4shows an amyloid b protein

Knowledge Base

Chart 2 shows amaloid b protein product

Knowledge Base

http://www.herbalextractok.com/HerbalExtract/Ginkgo_Biloba_extract.jpg

Figure 5 shows the plant of Ginkgo biloba

Lit Review

(Wu et, al, 2006)

PurposeTo determine if ginkgo biloba amyloid, freeze dried black raspberries and freeze dried blueberries has an effect on Alzheimer dieses in C. elegans.

Null hypothesis is that no effect will occur when ginkgo biloba amyloid, freeze dried black raspberries and freeze dried blueberries ware added.

The Alternative hypothesis is that ginkgo biloba amyloid, freeze dried black raspberries and freeze dried blueberries

Hypothesis

Methodology The Effect of Ginkgo Biloba Amyloid, Freeze Dried Black Raspberries and Freeze Dried Blueberries on the supression of Amaloid B in C. elegans

C. Elegans fed with a 10 % solution of Ginkgo Biloba N=?

C. Elegans fed with 10% solution of Freeze dried Black raspberriesN=?

C. Elegans fed with a 10 % solution of Freeze Dried Blueberries N= ?

C. Elegans will be fed with 100 ul spots of e. coli (OP5O) on nematode growth medium and c. elegans will be cultured a 20 degrees C

Results will be taken from visual analysis of C. elegans under a microscope.

C. Elegnas with no solutions N= ?(Control)

C. Elegnas with amaloid b and ginkgo biblia solution (Experimental)

Protocols for MeasurementGrown on NGM and C. elegans will be fed with E.

Coli (OP5O) with 100 ul spots.

Amaloid B (AB) protein will be mixed with and without ginkgo biblia at 100 ug/ml solution( stord at -20 Degrees C)

Ginkgo Biloba Amyloid, Freeze Dried Black Raspberries and Freeze Dried Blueberries will be given in the NGM in a 10 % solution.

Protocols for Measurement (cont)

All solutions will be given after one day and analysis will be looked at after 2 days.

Analysis will be compared ( under a microscope) to the number of normal and the number of paralyzed c. elegans

Analysis will be compared ( under a microscope) to the number of normal and the number of paralyzed c. elegans

Do- ability C. elegans and chemicals are easy to buyMuch of the equipment is already in the labChemicals are easily applied to C. elegans

Budget

Bulk Nutrition

Ginkgo Biloba powder

8.99 $8.99

ANA SPEC [Gln11] - beta - Amyloid (1 - 16)

25369 $90 $90

NutriFruit Freeze Dried Black Raspberry Powder

26-b8wl-f1ku

$25. 95 $25.95

NutriFruit Freeze Dried Blueberry Powder

BT-4AXL-SRXH

$20.95 $20.95

Vendor Item Item Number Cost per unit Total Cost

Budget (cont)Vendor Item Item

Number

Unit Cost Total Cost

Carolina Biological

Caenorhabditis elegans N2, Living, Culture

173500 $10.95 $10.95

Carolina Biological

Nematode Growth Agar

173520 $6.25(Quantity of 2)

$12. 50

Carolina Biological

Erwinia- Escherichia coli, living tube

155065 $9.20 $9.20

178.54

Bibliography Works Cited Hancock, Robert D. "Berry fruit as ?superfood?:." Scottish Crop

Research Group 54 (2007). Http://www.iob.org/userfiles/File/biologist_archive/Biol_54_2_Hancock.pdf.

Luo, Yuan. "Inhibition of amyloid -b aggregation and caspase- 3 activation by the Ginkgo biloba." Departments of Biological Sciences and Chemistry and Biochemistry (2002).

Wilson, Mark A. "Blueberry polyphenols increase lifespan and thermotolerance in Caenorhabditis elegans." PubMed Central (2006).

Wilson, Mark. "Bluebrry polyphenols increase the lifespan of c. elegans." Blackwell Publishing Ltd (2006).

Wu. "Egb reduces ab specific pathological behaviors are suppressed by ginkgo biloba extract Egb 761 in caenorhabditis elegans." (2006).

Yang, Huan. "The cytokine activity of HMGB1." Laboratory of Biomedical Science.