Rockets in front of IOLR Participation in SB – P1 (NIO) WP 0 – Project Managements WP 1 – Mechanisms for establishment of dormant stages WP 2 – Mechanisms

Rockets in front of IOLR

Participation in SB – P1 (NIO)

• WP 0 – Project Managements

• WP 1 – Mechanisms for establishment of dormant stages

• WP 2 – Mechanisms for arousal from dormant stages

• WP 4 – Engineering/Genetic testing strategies

• WP 5 – Discovery of common and divergent pathways for dormant stages, dormancy and desiccation resistance

• WP 6 – Dissemination of results

Workpackage 1 (WP1): Mechanisms for establishment of dormant stages

DeliverablesDeliverable

NumberDeliverable date

Optimal conditions for inducing asexually and sexually reproducing rotifers and their resting eggs

D4M9

cDNA libraries, EST sequencing D5M16

Pattern of global gene expression during formation of resting eggs by microarray analysis

D6M27

Quantitative analyses of the expression of specific genes by QPCR

D7M35

Proteins and metabolites identified to be associated with formation of resting eggs or contained within them

D8M36

P1- NIO: Goals for M6

WP1: TasksDeliverable

Number

Asexual and sexually reproducing rotifer cultures

D4M9

Construction of cDNA libraries (P5):

a) Asexually reproducing rotifers

b) Sexually reproducing rotifers

c) Subtractive library ??

EST’s (P5): ~ 5,000 clones per library

(~15,000 ESTs for rotifers)

D5 – Partner 5M16

Goals for M6-18

Workpackage 2 (WP2): Mechanisms for arousal from dormant stages



Patterns of global gene expression during exit from dormancy of rotifer resting eggs by microarray analyses

D11M35

Quantitative expression levels of specific genes during resumed development by QPCR

D12M35

Proteins and metabolites associated with resumed metabolic activities during embryonic development in restings eggs and asexual rotifer resting eggs

D13M30


Number

Resumed development of rotifer resting eggs- time course of events

D11M35

Goals for M12

Nadav’s presentation

Workpackage 1 (WP1): Mechanisms for establishment of dormant stages



Optimal conditions for inducing asexually and sexually reproducing rotifers and their resting eggs

D4M9

cDNA libraries, EST sequencing D5M16

Pattern of global gene expression during formation of resting eggs by microarray analysis

D6M27

Quantitative analyses of the expression of specific genes by QPCR

D7M35

Proteins and metabolites identified to be associated with formation of resting eggs or contained within them

D8M36


NumberMilestone

Collection of samples for global gene expression and QPCR analyses; amictic and mictic females from cultures at different stages of resting egg formation; resting eggs and amictic eggs

D6, D7M5

Collection of samples for proteome and

compound analyses; amictic and mictic

females from cultures at different

stages of resting egg formation; resting

eggs and amictic eggs

D8, D9M6

Goals for M12-18

Workpackage 2 (WP2): Mechanisms for arousal from dormant stages



Patterns of global gene expression during exit from dormancy of rotifer resting eggs by microarray analyses

D11M35

Quantitative expression levels of specific genes during resumed development by QPCR

D12M35

Proteins and metabolites associated with resumed metabolic activities during embryonic development in restings eggs and asexual rotifer resting eggs

D13M30


NumberMilestone

Collection of samples for global gene expression and QPCR analyses; resting eggs at 3 progressive stages of hatching

D11, D12M7


compound analyses; resting eggs at 3

progressive stages of hatching

D13, D14M8

Goals for M12-18

Workpackage 4 (WP4):Engineering/Genetic testing

strategies



Differences in the transcriptome, proteome and metabolome analyses between asexual and sexual rotifer strains

D20M35


NumberMilestone

Collection of samples for global gene expression and QPCR analyses of amictic females from a genetic line showing sexual reproduction and amictic females from a sexually reproducing culture

D20M12


compound analyses of amictic females

from a genetic line showing sexual

reproduction and amictic females from

a sexually reproducing culture

D20M12

Goals for M12-18

Workpackage 5 (WP5):Discovery of common and divergent pathways for

dormant stages, dormancy and desiccation resistance



Construction of the databasesD24M30

Identification of species- specific and conserved genes, proteins and compounds

D25M33

Development of in silco models for cell dormancy or desiccation tolerance

D26M36

Production of a comprehensive reportD7M35


Number

Compile information on gene and cDNA sequences of rotifers for data base

D24M30

Goals for M12-18

Workpackage 6 (WP6):Dissemination of results



Participation in scientific meetings D28M36

A workshop on dormancy: opportunities and challenges: preservation of cells and organisms

D29M24*

Publication of a book on dormancy D30M36*

* To be discussed at the meeting

WP 6: TasksDeliverable


Participation in scientific meetings and publication of manuscripts

D28M36

Organization of the workshop- application for additional financial support

D29M24*

* To be discussed at the meeting

Goals for M12-18

Documents

Rockets in front of IOLR Participation in SB – P1 (NIO) WP 0 – Project Managements WP 1 – Mechanisms for establishment of dormant stages WP 2 – Mechanisms