University of StirlingSchool of Biological & Environmental
SciencesLaboratory Risk Assessment
FormInstructionsforcompletingform:if usingMSWordviewasNormal
Layout, zoomto100%.Type information witin te !o"es provided.Tese
will e"pand to a##ommodate tete"t.$efore printing #e#% &ile,
page setup, page size is '(.1. Title of Work Activity.etermination
of soil e!changeable cations an" cation e!change ca#acity2.
escri#tion of Work Activity (describe Method/Working
Procedure/Protocol, including safety precautions and emergency
procedures where necessary).Exchangeable cations and cation
exchange capacity1.0 M potassium chloride Dissolve 74.56g KCl in
distilled water. Transfer to a 1000ml flask and maketo volume with
distilled water.0.01M sodium hydroxide Dissolve 0.4g Na! in
distilled water. Transfer to a 1000ml flask and maketo volume with
distilled water. "re#are in a fume $u#%oard.Strontium
nitrate/hydrochloric acid solutionDissolve 10 g strontium nitrate
in distilled water. Transfer to a 100 ml volumetri$ flask and
dilute to volume with distilled water. "i#ette &.' ml $on$.
h(dro$hlori$ a$id into a 100 ml flask half full of distilled water.
Cool and dilute to volume. )i* the twosolutions in a reagent
%ottle. "re#are in a fume $u#%oard.phenolphthalein indicator
Dissolve 1g of #henol#hthaleinin 100ml ethanol then mi* with 100ml
distilled water stirring $onstantl(. +ilter if a #re$i#itate forms
and transfer to a reagent %ottle.0.5 and 1 mmolc L-1 Mg
standards"i#ette 0.6 and 1., m- sto$k )g standard solution into 100
m- flasks. "i#ette 4 ml strontium nitrate.h(dro$hlori$ a$id mi*ture
into ea$h flask and make to volume with KCl solution.1 and 2 mmolc
L-1 a standards "i#ette , and 4 m- sto$k Ca standard solution into
100 ml volumetri$flasks. "i#ette 4 ml strontium
nitrate.h(dro$hlori$ a$id mi*ture into ea$h flask and make to
volume with KCl solution. /*$hangea%le $ations are dis#la$ed %(
lea$hing soil with a solution of #otassium $hloride. 0n the
lea$hate1 e*$hangea%le $al$ium and magnesium are determined %(
atomi$ a%sor#tion s#e$trometr( and e*$hangea%le a$idit( %(
titration against standard sodium h(dro*ide solution. 2e will
ignore the generall( small $ontri%utions from e*$hangea%le Na and K
and $al$ulate effe$tive C/C as the sum of e*$hangea%le Ca1 )g and
a$idit(.i. 2eigh 5 g 340.01 g5 of dr( soil into a $lean 50 ml
%eaker. 6dd ,5 ml #otassium $hloride 3KCl5 solution and stir
thoroughl(.ii. "la$e a filter #a#er in a funnel over a 100 ml
$olle$ting %ottle. "our the soil sus#ension through the filter.
2hen the li7uid has drained1 lea$h the soil with 8 further ,5 ml
#ortions of KCl1 allowing ea$h to drain %efore adding the ne*t
3total of 100 ml lea$hing solution5. 9etain the lea$hate for
anal(sis of Ca1 )g and a$idit( on$e drainage has sto##ed.iii.
Determination of e*$hangea%le Ca and )g :sing a measuring $(linder1
transfer ,5 ml of the lea$hate to a $lean %eaker and add 1 ml
strontium nitrate solution. Determine the $on$entrations 3as mmol$
l;15 of Ca and )g in the lea$hate %( atomi$ a%sor#tion
s#e$tro#hotometr( 366< 3?5=H Mg Ca CEC + + =CECMg CaBaseSat5 3
100 +=!. Location (list laboratories in which work will be carried
out).$U$% &'( an" (B)**". The follo+ing gro,#s of #ersons may
be at risk- Aca"emics Technicians St,"ents 'isitors .leaners
/aintenance 5. 0a1ar"o,s /aterials ,se" as Ra+ /aterials (Complete
C!""/#isk $ssessment !ummary !heet. %ist all compounds, which are
harmful, irritant, corrosi&e, flammable, to'ic, teratogenic,
and carcinogenic or ha&e an (% or M(%, microorganisms, dust
etc. )ick the appropriate bo'es for ha*ard classification, route of
contact and personal protecti&e e+uipment).See anne!
sheet#.0a1ar"o,s s,bstances create" as interme"iates or
by2#ro",cts.3one$. /etho" of is#osal of Waste. Categorise waste as
laboratory drain, normal landfill or specialist disposal.
Categories for specialist disposal are i) sol&ents
(non,chlorinated hydrocarbons and alcohols, chlorinated
sol&ent, acetonitrile or others. -... sol&ents containing
acetonitrile, ben*ene and tetrahydrofuran must be kept separately,
ii) solids (acid, o'idising and to'ic). Consult the !afety
$d&iser if you are in any doubt about which compounds are
compatible or the correct method of disposal.)he following persons
are responsible for the disposal of specialist wastes/ %. Risks
4"entifie" in the Work Activity. (%ist all C!"" associated risks
and others such as/ , flammability, naked flames, manual handling
etc. below, together with the protecti&e measures re+uired).
#0!1 P#(2(-)$)02( M($!3#(!!odium hydro'ide solution/ corrosi&e
Wear lab coats, glo&es and use eye protection. Prepare reagent
in fume cupboard.$tomic absorption spectrophotometer/ naked flame
and compressed gases0nstrument should be set up by a technician or
other competent member of staff and operated only under
super&ision."ydrochloric acid/ corrosi&e Wear lab coats,
glo&es and use eye protection. Prepare reagent in fume
cupboard.Laboratory Risk Assessment Form Ver. 1.0"age , of 5&.
0as a +ritten Emergency 5roce",re been #re#are"6 4es-o 0f so it
should be attached to this form or incorporated into the Written
Working Procedure (section 5). )his should include procedures for
the emergency shutdown of e+uipment, dealing with spillages of
large amounts of chemicals (especially corrosi&es and
&olatiles) or the mi'ing of incompatible chemicals and
specialist first aid treatment (e.g. for phenol burns).S#illages of
aci" sho,l" be ne,tralise" imme"iately +ith so"i,m carbonate
#o+"eran" the area thoro,ghly +ashe" ,# +ith +ater.S#lashes of aci"
on the skin sho,l" be irrigate" imme"iately +ith #lenty col"
+ater.S#lashes of aci" to the eye sho,l" imme"iately be irrigate"
thoro,ghly +ith +ater from an eye irrigation #oint.Seek me"ical
attention for any aci" b,rns.4nstr,ctions for emergency sh,t"o+n of
the AAS are #rovi"e" besi"e the instr,ment. 5ress the re" b,tton to
initiate safe sh,t"o+n an" t,rn off gases at cylin"er +hen the
flame is e!ting,ishe".10. 4s S#ecial Training or S,#ervision
Re7,ire"64es-o )he super&isor will appro&e straightforward
work in progress)he super&isor will specifically appro&e
the scheme of work in section 5)he super&isor will pro&ide
super&ision personally to control the work6urther information
(if yes selected)/AAS m,st be o#erate" by a laboratory technician
or other com#etent member of staff11. 4f this #roce",re is
restricte" to name" in"ivi",als #lease name them belo+.12.
Restrictions on ho,rs of +ork..an any of these #roce",res be
carrie" on 8o,t of ho,rs9 or by lone +orkers6
4(! -%one working6irst aid pro&ision essentialut of hours (7
pm 8 9.:; am) permitted$re there any additional precautions, which
need to be taken for 1!. When sho,l" this assessment an" the
control meas,res ne!t be revie+e"6 ?ate/ @anuary
5;;A)(.Accre"itation.!ignature of $ssessor/-ame/ r 4an :rieve?ate/
:B @anuary 5;;7Laboratory Risk Assessment Form Ver. 1.0"age 8 of
5
Laboratory Risk Assessment Form Ver. 1.0"age 4 of 5);. User
Training Recor"Trainee-0 ha&e read and fully understood the
risk assessment prepared for the abo&e work acti&ity. 0
understand the risks created and agree to use the control measures
specified. 0 ha&e recei&ed the training necessary to carry
out the work.S,#ervisor-0 am satisfied that this person has been
trained and is capable of carrying out the work described.)rainee
signature )rainee name (printed) !uper&isor signature
?ateLaboratory Risk Assessment Form Ver. 1.0"age 5 of 5
