RIKEN 2017 JOINT RETREAT

FEBRUARY 2 and 3, 2017

Visualization

Organizers:

Yoshihiro Yoshihara, Brain Science Institute

Tomomi Shimogori, Brain Science Institute

Atsushi Mochizuki, Institute Laboratories

Yoichi Shinkai, Institute Laboratories

Carina Hanashima, Center for Developmental Biology

Ichiro Hiratani, Center for Developmental Biology

Kam Zhang, Center for Life Science Technologies

Mariko Okada, Center for Integrative Medical Sciences

Yoshihiro Shimizu, Quantitative Biology Center

Notice

Joint Retreat is a closed meeting in which active discussion on

unpublished results and ideas is encouraged. All information

presented should be treated as confidential by attendees.

General Information ························································ 3

Program ····································································· 11

Abstracts for Session I Oral Presentation ·························· 13

Abstract for Educational Lecture ······································ 16

Abstract for Guest Speaker’s Presentation ························ 17

Abstracts for Session II Young Investigators’ Talks ·············· 18

Abstracts for Session III Oral Presentation ························ 23

Order of Poster Short Talks ············································ 26

Poster Session Layout ·················································· 28

Authors and Titles for Poster Presentations ······················· 29

Participant List ····························································· 46

Contents

－1－

－2－

Shinkansen (Hikari/Kodama)

nkansenShinkansen

Shinkansen (Hikari)

About 1 hour 40min. About 2 hours from Tokyo

※ by bus about 30 min.

1. Date February 2-3, 2017

2. Venue Hamanako Royal Hotel

4396-1 Yamazaki, Yuto-cho, Nishi-ku, Hamamatsu-shi, Shizuoka 431-0101

URL: http://www.daiwaresort.jp/en/hamanako/index.html/

TEL:053-592-2222

Map：Hamanako Royal Hotel

Shinkansen timetable: http://english.jr-central.co.jp/info/timetable/index.html

Meeting Place & Time

Please come to JR Hamamatsu Station at 12:00 on Feb 2. Access

●By train

General Information

Shin-Osaka,

Shin-Kobe

Stations

Hamamatsu

Station Tokyo,

Shinagawa,

Shin-Yokohama

Stations Hamanako Royal Hotel

－3－

Meeting Place&Time at Hamamatsu station

If you arrive there later than at around 12:15, you should take JR Tokaido Honsen* (see time table next page) local train to JR Maisaka Station and then take a taxi out of your own pocket (or take a hotel shuttle bus, limited operation)

Hotel Shuttle Bus timetable

Lv. JR Maisaka 11:15 14:00 15:00 16:00

After the retreat, the busses will take you back to JR Hamamatsu Station.

To Yokohama / Tokyo

To Osaka / Kobe

Hamamatsu station

North Exit

★HERE! 12:00 In front of ACT city

South Exit

－4－

*JR Tokaido Honsen Timetable

●For participants from Yokohama/Shinagawa/Tokyo Hamamatsu and Maisaka Station JR Tokaido Honsen Timetable Outward (bound for Toyohashi / Gifu) Return (bound for Hamamatsu / Kakegawa)

Hamamatsu Sta. Maisaka Sta. Maisaka Sta. Hamamatsu Sta.

11:03 11:13 15:05 15:15

11:20 11:30 15:29 15:39

11:44 11:54 15:49 15:59

12:03 12:13 16:05 16:15

12:20 12:30 16:24 16:34

12:43 12:53 16:48 16:58

●For participants from Kobe/Osaka Toyohashi and Maisaka Station JR Tokaido Honsen Timetable Outward (bound for Hamamatsu / Kakegawa) Return (bound for Toyohashi / Gifu)

Toyohashi Sta. Maisaka Sta. Maisaka Sta. Toyohashi Sta.

10:42 11:06 15:13 15:39

11:03 11:27 15:30 15:56

11:23 11:47 15:52 16:18

11:42 12:06 16:13 16:39

12:03 12:27 16:30 16:56

12:23 12:47 16:50 17:16

●Taxi Reservation from Maisaka Station ⇒ Entetsu Taxi TEL： 053-412-7777

－5－

3. Official language: English

4. Registration 11:30 to 13:00 on February 2nd. The registration desk is located in front of the SWAN. All participants

are requested to sign in, pay the registration fee and pick up their name card.

Participants are required to wear the Name card throughout the Joint Retreat. 5. Registration fee Registration fee will be collected on the event day. Please prepare cash. Participation fees include accommodation, breakfast, banquet and lunch (only 2nd day).

Please bring cash with you!!

Management and higher rank employees (Kanri-shoku): 17,150 JPY Others: 15,150 JPY (150 JPY is hot spring tax) ※ Additional 4,000JPY is required for a single room request.

Note: Expense for accommodation, meals and travelling will be covered by each lab budget. Please follow the business trip procedures of your centers. 6. Hotel facilities Hotel Floor Plan

13F Restaurant, Guest Rooms 5-12F Guest Rooms 4F Conference Rooms Poster Session 3F Japanese-style Banquet Halls, Game Corner 2F Restaurant “Shiki”, Royal Hall (SWAN), Western-style Banquet

Rooms, Seminar Hall, Dining Room, Wedding Hall 1F Front, Lobby, Restaurant, Shopping Plaza, Hot Spring Large

Japanese Bath, etc. For detail, please see the hotel official website: http://www.daiwaresort.jp/en/hamanako/facilities/index.html/#a04 (1) Internet environment The hotel provides free Wi-Fi service for guests. Choose a network name “hamanakorh”. (2) Shopping Plaza Regional specialty products, fresh seafood, locally-brewed sake, crafts, and other souvenir goods fill this large shop. There is also a selection of soft drinks, alcohol, and sweets for enjoying in your room. Floor: 1F Open: 7:00-21:00 (3) Vending machines

－6－

Vending machines are located on Floor 1 and Floors 5-12 near Room 30. (4) Bar, Lounge A menu of drinks and light meals is available. - Tea lounge "Ripple"

Floor: 1F Open: 8:00-18:00 - Sky bar "Séduire"

Floor: 13F Open: 20:00-22:00 For detail, please see the hotel official website: http://www.daiwaresort.jp/en/hamanako/facilities/index.html/#a02 (5) Hot springs - Open-air hot spring rock bath

Floor: 1F Open: 5:00-10:30 / 11:30-25:00 - Indoor hot spring communal bath

Floor: 1F Open: 5:00-10:30/11:30-25:00 For detail, please see the hotel official website: http://www.daiwaresort.jp/en/hamanako/facilities/index.html/#a03 7. Accommodation a) Check in Only 1 room key is available per room. A group leader is assigned per each room. The leader must pick up the room key at the registration desk at SWAN after the poster session on February 2 and open the room. All participants should drop off your bags and meet your room mates between 19:00 to 19:30. b) Check out Check out the room by 09:00 on February 3. Keys should be returned directly to the front desk. Payment of the hotel room is NOT required. However, the cost of personal expenses such as use of phones, must be paid for separately at the time of check out by the individuals. c) Amenities Every guest room has its own bath and toilet and comes complete with various amenities, including bath towels, face towels, yukata robes, tooth brush (tooth paste), shampoo, conditioner, body soap, hair dryer among other things.

8. Meals

Lunch (Feb. 2) Participants must arrange their own lunch on Feb 2.

Banquet (Feb. 2) Banquet starts at 19:30 at “Shiki”

Breakfast (Feb. 3) Breakfast is served from 7:30 to 9:00 at “Shiki”

Lunch (Feb. 3) Lunch starts at 11:55 at "Shiki" on the 2nd floor

－7－

9. Presentation Oral Presentation: A screen, an LCD projector and laser pointer will be provided in the room. Be sure to bring any peripherals or adaptors needed to connect your computer to the projector and/or save your presentation on USB flash drive. Check the connection at the operator desk near the podium before each session of your presentation. Poster Short Talks: Each presenter has two (2) minute talk time. Presenters should submit one page (A4 size) slide (pdf.) data in advance to the Joint Retreat Administration Office. All the presenters’ data is saved on one PC provided by the Administration Office. The presenters (alphabetical order) use the PC for smooth transition between the presenters. Poster Presentation: The size of the poster board is 90 cm (Width) X 210 cm (Height). A 20 cm X 20 cm space in the upper left corner should be left blank for numbering the panel. The poster number will be provided by the organizing office.

Posting: February 2, 11:30-13:00 Aquamarine/Sapphire (4F)

Removal: February 3, 10:00-10:10 Aquamarine/Sapphire (4F)

Poster Session I: Feb. 2, 17:30-19:00 (17:40-18:40 core time)

Poster Session II: Feb. 3, 09:00-10:10 (9:00-10:00 core time) Poster session room will be open until 23:30 on February 2nd for free discussion. Participants who are leaving in the middle of the program should remove the poster at their time

of departure.

! IMPORTANT NOTICE ! The abstracts in this booklet should not be cited in bibliographies. Abstracts and the contents of oral and poster presentations delivered at the Joint Retreat should be treated as personal communications, and should not be cited without authors’ consent.

－8－

Floor Map

Emergency Exit

*Please confirm emergency exit when entering the room.

Front desk

Ext: #9

Spa

Spa

Women

Men

Shop

*7:00-21:00

*Spa: 5:00-10:00

12:00-25:00

SWAN

Dining

Hall

Internet spot

Smoking area

*1st Day Dinner

2nd Day Breakfast &Lunch

Stairs

Stairs

*Poster

Sessions

& Social

Gathering

(21:00-)

Smoking area

*Non-smoking

Lobby

*Photo shooting

(2nd day)

Lecture

●Front Desk

●Spa

●Shop

●Vending Machine

●Smoking Room

●Front Desk

●Spa

●Shop

●Vending Machine

●Smoking Room

Lobby

●Meeting Room ●Guest Rooms*Non-smoking

Lobby

Elevator

Elevator

Elevator

Elevator

Royal Hall

“Shiki”

Stairs

－9－

Vicinity Map

To JR Maisaka Sta. 3.5km from hotel

Grocery store Y shop Open: 9:00-18:00

Closed: Wednesday 0.6 Km from hotel

Convenience store Open: 24hours

1.1km from hotel

－10－

Time Event Place

11:30- Registration and Poster setup Lobby in front of SWAN (2F)

13:00-13:10 Opening Remarks Yoshihiro Yoshihara (BSI)

SWAN(2F)

Session I Oral presentation (Presentation 20min+ Q&A 10min) Chairperson: Yoshihiro Shimizu (QBiC)

13:10-13:40

Title Involvement of PD1 in antibody diversification and immune homeostasis

Speaker Sidonia Fagarasan (Abs. No. SI-1)

Lab Lab. for Mucosal Immunity, IMS

13:40-14:10

Title Visualization of biological complexes by electron cryo-microscopy

Speaker Hideki Shigematsu (Abs. No. SI-2)

Lab Protein Function and Structural Biology Team, CLST

14:10-14:40

Title Endothelial actin dynamics during blood vessel morphogenesis

Speaker Li-Kun Phng (Abs. No. SI-3)

Lab Lab. for Vascular Morphogenesis, CDB

Educational Lecture Chairperson: Tomomi Shimogori (BSI)

14:40-15:10

Title Visualization approaches for scientific manuscript writing

Speaker Charles Yokoyama (Abs. No. SI-4)

Lab Director’s Office, BSI

15:10-15:30 Coffee Break Lobby in front of SWAN

Guest speaker’s presentation (Presentation 40min+Q&A 10min) Chairperson: Mariko Okada (IMS)

15:30-16:20

Title Studies on biomolecules using single-molecule imaging techniques

SWAN(2F) Speaker Yoshie Harada

Affiliation Institute for Protein Research, Osaka University

16:20-17:10 Poster Short Talks

17:10-19:00 Poster Session (17:40-18:40 Odd Numbers Core Time) Aquamarine, Sapphire (4F)

19:00-19:30 Room Key Pick-up Lobby in front

of SWAN(2F)

Check in 5F-12F Guest rooms

19:30-21:00 Dinner Shiki (2F)

21:00-23:30 room close Social Gathering Aquamarine,

Sapphire (4F)

Program Day 1, Feb. 2, 2017

－11－

Program Day 1, Feb. 2, 2017

Time Event Place

7:00-9:00 Morning Buffet Shiki (2F) -9:00 Check out Front Desk

9:00-10:00 Poster Session (Even Numbers Core Time) Aquamarine, Sapphire (4F) 10:00-10:10 Removal of posters

Session II Young Investigators' Talks (Presentation 15min+ Q&A 5min) Chairperson: Carina Hanashima (CDB)

10:10-10:30 Title Dissection of neural circuitry mediating CO2-evoked

escape behavior in the larval zebrafish

SWAN(2F)

Speaker Tetsuya Koide (Abs.No.SII-1) Lab Laboratory for Neurobiology of Synapse, BSI

10:30-10:50

Title 4D live cell imaging of membrane traffic Speaker Kazuo Kurokawa (Abs.No.SII-2)

Lab Live Cell Super-Resolution Imaging Research Team, RAP

10:50-11:10 Title Interactive visualization and analysis using ZENBU

system Speaker Jessica Severin (Abs.No.SII-3) Lab Division of Genomic Technologies, CLST

11:10-11:30 Title Evolution of your IQ and the high price you pay to have it Speaker Pavel Prosselkov (Abs.No.SII-4） Lab Laboratory for Behavioral Genetics, BSI

11:30-11:50 Title Real time 3D in vivo pH imaging of stratum corneum

revealed complex morphology-based regulation in mice Speaker Yuki Furuichi (Abs.No.SII-5) Lab Laboratory for Skin Homeostasis, IMS

11:50-11:55 Photo shooting Lobby of the Hotel Entrance (1F)

11:55-13:00 Lunch Shiki (2F) Session III Oral presentation (Presentation 20min+ Q&A 10min) Chairperson: Atsushi Mochizuki (ILs)

13:00-13:30 Title High-resolution mapping of neuronal circuits with light

microscopy

SWAN(2F)

Speaker Takeshi Imai (Abs.No.SIII-1) Lab Laboratory for Sensory Circuit Formation, CDB

13:30-14:00 Title

Systems biology of mammalian sleep/wake cycles ~Involvement of Ca2+-dependent hyperpolarization in sleep duration in mammals~

Speaker Hiroki Ueda (Abs.No.SIII-2) Lab Laboratory for Synthetic Biology, QBiC

14:00-14:30

Title TBA

Speaker Atsushi Miyawaki (Abs.No.SIII-3)

Lab Laboratory for Cell Function Dynamics, BSI

14:30-14:40 Closing Remarks Shigeo Koyasu, Executive Director 15:00 Bus departure, 15:30 Hamamatsu Station (expected)

Program Day 2, Feb. 3, 2017

－12－

No.SI-1 February 2nd

13:10-13:40

Session I Oral Presentation

Involvement of PD1 in antibody diversification and immune homeostasis

FAGARASAN Sidonia

Laboratory for Mucosal Immunity, RIKEN Center for Integrative Medical Sciences (IMS)

The main function of the immune system is to protect the host against pathogens, such as bacteria or viruses. However, unlike the systemic immune system, the gut immune system does not eliminate microorganisms but instead nourishes rich bacterial communities and establishes advanced symbiotic relationships. Not only that the gut bacteria are essential for nutrient processing, production of vitamins and protection against pathogens (through competition for space and nutrients) but the development and maturation of the immune system depends on these bacteria.

Our previous studies demonstrated that the absence of immunoglobulin A (IgA) (the major effector molecule of the adaptive immunity in the gut), or the impaired IgA selection in germinal centers (GC) due to deregulated T cell control, severely affects the balance of gut bacterial communities, resulting in massive activation of the whole body immune system. Together the results point to an important role played by the adaptive immune system in regulating the microbial communities in the gut. I will discuss the role of the inhibitory co-receptor programmed cell death (PD)-1 in IgA diversification required for the maintenance of gut homeostasis and prevention of autoimmunity.

－13－

No.SI-2 February 2nd

13:40-14:10

Visualization of biological complexes by electron cryo-microscopy Hideki Shigematsu

Protein Functional and Structural Biology Team, Center for Life Science Technologies

Electron cryo-microscopy (cryoEM) is the way to visualize biological macromolecular

complexes embedded in ice by using transmission electron microscopy (TEM). TEM is a

powerful tool to magnify and visualize specimens at sub-angstrom resolution. The

method so called single particle analysis in cryoEM utilizes such a powerful tool to

depict individual molecules embedded in ice as particles. We utilize purified biological

complexes as a specimen for this analysis, therefore we assume all the particles appeared

in the data corresponds to the same three-dimensional (3D) shape. This is the reason why

we call this method as single particle analysis. The recent advances in development of

detectors and software algorithm allow us to resolve the 3D structures of biological

complexes in near atomic resolution. This was called resolution revolution in a review

article on Science and the cryoEM was selected as a “method of the year” in 2015 by

Nature Methods. Nowadays, you will see many of articles on the top journals every

week for the cryoEM single particle analysis and still the field is growing.

We have introduced state of the art cryoEMs in Yokohama campus at the end of fiscal

year of 2013 and have been working on collaborative research with inside and outside

RIKEN researchers. I will present some results already published and will talk about

recent progress in methodology development in single particle cryoEM to visualize

membrane protein structure embedded in liposomes. Our goal for the current project is

to understand voltage gating mechanism of ion channels. For this purpose, we employed

liposome as a lipid environment for voltage gating ion channels to be embedded.

Because of its spherical shape, we can apply membrane potential by changing the

outside buffer with ion channels. Therefore, our aim is to depict structural differences

with and without membrane potential to observe conformational changes in voltage

gating sensors.

－14－

No.SI-3 February 2nd

14:10-14:40

Endothelial actin dynamics during blood vessel morphogenesis Li-Kun Phng

RIKEN Center for Developmental Biology, Laboratory for Vascular Morphogenesis

Blood vessels, together with the heart, have a fundamental role in transporting

oxygen and nutrients to support the metabolic demands of tissues and organs not only

during development, but also in adults. In order to serve this function, blood vessels

must form and maintain lumen so that an interconnected network of tubules spans the

entire body to infiltrate organs and tissues. Many blood vessels form through the process

of sprouting angiogenesis, when endothelial cells lining pre-existing vessels become

activated to form new vascular sprouts. The new vascular sprout grows through

polarised collective cell migration, cell elongation and cell proliferation, and, in order to

generate a continuous network of vessels, endothelial tip cells fuse through the process

of anastomosis. Concomitantly, the process of lumen formation takes place to render

blood vessels functional. Thus, endothelial cells exhibit a multitude of cellular processes

that are tightly coordinated during vessel development.

Endothelial cells are highly plastic in their ability to change their morphology to

drive specific cellular processes. In order to understand how this plasticity is achieved,

we investigated of how the actin cytoskeleton regulates endothelial membrane dynamics

during angiogenesis in the zebrafish embryo. During the expansion of blood vessels, the

generation of actin bundles in filopodia facilitates efficient collective cell migration and

anastomosis. During lumen formation, transient polymerization of F-actin at the apical

membranes controls lumen expansion, while a stable pool of actin cables at endothelial

cell junctions stabilizes nascent lumens to produce a functional vascular network. Thus,

actin cytoskeleton of different subcellular localization and dynamics drive distinct steps

of vessel morphogenesis.

－15－

February 2nd

14:40-15:10

Educational Lecture

Visualization Approaches for Scientific Manuscript Writing Charles Yokoyama, Ph.D. Director for Research Administration, Brain Science Institute A single piece of scientific knowledge, such as a paper, is defined by a lineage and

coherence. Similar pieces aggregate into knowledge domains that in turn form a

conceptual landscape serving as a comprehensive ontology of scientific history and a

“road map” for future investigation. Two practical challenges for researchers navigating

at the frontiers of this knowledge landscape are (1) How to read existing conceptual maps

and choose important and interesting directions to pursue within a limited career span (2)

How to write effective and useful research manuscripts that add significant knowledge

and value to the conceptual map. In this lecture, we will discuss the structures of

scientific concepts, and how the resultant projects and papers interact with the publication

process. We will explore how visualization approaches in manuscript writing can help to

facilitate knowledge formation and provide the basis for a future ontology-based science.

－16－

February 2nd

15:30-16:20

Guest Speaker’s Presentation

Studies on Biomolecules Using Single-Molecule Imaging Techniques Yoshie Harada

Institute for Protein Research, Laboratory of Nanobiology, Osaka University

The best way to obtain unambiguous information about the function of biomolecules is to

study their function at the single-molecule level. Twenty years ago, we developed single

fluorescent dye molecule imaging technique using fluorescence microscope which reduced

background noise dramatically by installing evanescent illumination. Using this technique,

we observed the sliding movement of single kinesin molecules along a microtubule and

also visualized binding of single RNA polymerase molecules to a single DNA molecule.

However, due to the background noise, even if using the evanescent illumination, we are

not able to observe a single fluorescent dye molecule under higher than 50 nM fluorescent

dye concentration conditions. To investigate dynamic processes of DNA-protein

interactions related to genome DNA maintenance we are employing zero-mode

waveguides (ZMWs). ZMWs are nano-hole (100 nm in both depth and diameter) arrays on

an aluminum coated fused silica substrate. ZMWs allow single-molecule observation under

several μM fluorescent dye concentration conditions due to its extremely small excitation

volume (~ 10-21 L). Using ZMWs, we focus on characterizations of proteins involved in

homologous recombination or epigenetics such as RuvAB protein complex or nucleosome

binding proteins. Currently we are also developing a new single-molecule imaging

technique using fluorescent diamond nanoparticles. Fluorescence of nitrogen-vacancy

centers (NVC) embedded in nanodiamonds exhibits neither photobleaching nor blinking,

superior to the fluorescence properties of widely-used organic dyes and quantum dots.

Another uniqueness of NVC is that the fluorescence intensity can be modulated by

magnetic resonance technique. It is based upon a presence of energy levels partly shared by

both processes of the fluorescence-emission and of electron spin resonance. By using the

property we developed a technique that can selectively detect single NVC out of an

autofluorescence.

－17－

Dissection of Neural Circuitry Mediating CO2-evoked Escape Behavior in the Larval Zebrafish Tetsuya Koide and Yoshihiro Yoshihara

RIKEN Brain Science Institute, Japan

Avoidance of carbon dioxide (CO2) is a vital reaction commonly preserved in various

animal species from worms to mammals for their survival. In this study, we aim to

elucidate the neural circuit mechanism underlying CO2 avoidance in larval zebrafish.

First, we observed that the application of CO2 to larval zebrafish elicits a robust escape

response. Next, we performed in vivo whole-brain Ca2+ imaging to map neural

substrates governing CO2-evoked escape response. Upon CO2 application to the nose,

ensembles of neural activation were observed in multiple brain regions, including the

olfactory bulb (OB), the trigeminal ganglion (TG), and the hindbrain. Because the fish

nose receives two distinct types sensory information, olfactory and trigeminal, we

laser-ablated either the OB or the TG and performed Ca2+ imaging and behavioral

analysis to examine each contribution to the escape response. As a result, the

CO2-evoked neural activation in the hindbrain and escape behavior were markedly

decreased in the TG-ablated fish, but not in the OB-ablated fish. These findings

demonstrate that the nasal trigeminal pathway is responsible for CO2-evoked escape in

larval zebrafish.

Young Investigators’ Talks

－18－

No. SII-2 February 3rd 10:30-10:50

4D Live Cell Imaging of Membrane Traffic Kazuo Kurokawa1), Midori Ishii1) 2), Akihiko Nakano1)2)

1) Live Cell Super-Resolution Imaging Research Team, RIKEN, Japan, 2) Laboratory

of Developmental Cell Biology, University of Tokyo, Japan

Membrane traffic is an essential mechanism for transport of proteins and other

macromolecules to various destinations inside or outside of the cells. It also underlies

the fundamental need for cells to maintain cellular homeostasis. Membrane traffic is

mediated via membrane-surrounded vesicles and carrier intermediates. The basic

mechanism of membrane traffic is conserved in all eukaryotic cells, from yeast to higher

animals and plants. The Golgi apparatus is a central station of this traffic. Newly

synthesized proteins in the ER are delivered to the Golgi apparatus in which they are

modified and selectively sorted to their final destination.

We have conducted 4D live cell imaging at high spatiotemporal resolution, which is a

promising approach to resolve many remaining problems of membrane traffic. For this

purpose, we have developed SCLIM (super-resolution confocal live imaging

microscopy) by combining inverted fluorescence microscope with a high-speed and

high-signal-to noise-ratio spinning-disk confocal scanner, a custom-made spectroscopic

unit, image intensifiers, and multiple EM-CCD cameras for multiple color observation.

Using this system, we have investigated 1) ER-Golgi protein traffic, 2) the mechanism

of the Golgi maturation, 3) intra-Golgi protein traffic in living Saccharomyces

cerevisiae.

－19－

No. SII-3 February 3rd

10:50-11:10

Interactive visualization and analysis using ZENBU system

Jessica Severin, Michiel De Hoon, Alistair Forrest, Piero Carninci

RIKEN CLST

Genome-wide compendium studies, such as FANTOM, ENCODE, Roadmap, TCGA

and single-cell studies are providing new challenges for visualization, analysis and

secured data sharing due to their unprecedented breadth and scale. The ZENBU system

(Severin et.al. Nature Biotechnology, 2014) enhances the genome browser concept by

integrating advanced, on-demand data processing and analysis with interactive

visualization optimized for comparison across 1000s of secured experimental samples.

Any user of the system can create secured collaborations, manage membership, and

upload 1000s of experimental data files with descriptive metadata. Through the

visualization interface, researchers can choose experimental data, modify analysis

parameters and scripts, quickly see the newly processed result on a genomic region of

interest, apply the analysis genome wide, and share the visualization and data export

results with their collaborators.

ZENBU‘s interactive visualization can dynamically combine thousands of experimental

datasets in a multi-dimensional matrix allowing the researchers to visually slice and

group the dataset through linked genome location, experimental signal and metadata

views. ZENBU is open-source and freely available for use on the web

http://fantom.gsc.riken.jp/zenbu and for installation in individual laboratories.

－20－

No. SII-4 February 3rd 11:10-11:30

Evolution of your IQ and the High Price you pay to have it Pavel Prosselkov1)2), Ryota Hashimoto3)4), Denis Polygalov5), Kazutaka Ohi3), Qi Zhang1), Hiromichi Goto1), Thomas J. McHugh5), Masatoshi Takeda3)4), and Shigeyoshi Itohara1)

1) Laboratory for Behavioral Genetics, RIKEN Brain Science Institute, Japan 2) Grad School, Department of Veterinary Medicine, Tokyo University, Japan 3) Department of Psychiatry, Osaka Univ Graduate School of Medicine, Japan 4) United Graduate School of Child Development, Osaka University, Japan 5) Lab for Circuit and Behav Physiol, RIKEN Brain Science Institute, Japan

Around 500 million years ago a massive genome duplication event, termed the

Cambrian explosion, took place. Many genomes underwent a gene duplication event,

subfunctionalization, and de novo functional elaboration. This provided animals with a

capacity to occupy challenging ecological niches (e.g. the land) through the positive

selection of advantageous anatomical and cognitive traits. Among the duplicated gene

paralogs were NTNG1 and NTNG2, brain-specific presynaptic proteins found only in

vertebrates. These genes have been implicated in IQ determination in human subjects,

with a unique labor division, such that NTNG1 affects verbal comprehension and

processing speed while NTNG2 impacts working memory and perceptual organization.

Non-coding gene loci responsible for this effect underwent accelerated evolution from

primates through Neanderthals/Denisovans to modern humans. Simultaneously, both

NTNG genes became associated with a variety of neurocognitive disorders, co-

segregating with some complex etiologies (e.g. schizophrenia and autism), while

individually and exclusively segregating within others (e.g. Alzheimer and lupus). In

mice both genes affect executive function, contributing to attention and working

memory, top-down (Ntng2, cognition) and bottom-up (Ntng1, perception) information

flow, in a manner similar to the human NTNG gene orthologs. Such “cognitive

complementarity” underscores an evolutionary gain-dependent link between advanced

cognition and concomitant propensity to develop neuropathologies. This risk/reward

trade-off implies that as living creatures we may have reached the maximum threshold

of our brain capacity.

－21－

No. SII-5

February 3rd 11:30-11:50

Real Time 3D in vivo pH Imaging of Stratum Corneum Revealed Complex

Morphology-based Regulation in Mice

Yuki Furuichi 1) 2), Takeshi Matsui 2), Masayuki Amagai 1) 2)

1) Department of Dermatology, Keio University School of Medicine, Japan, 2)

Laboratory for Skin Homeostasis, RIKEN, Japan

The regulation of pH is an important element in various biological functions. Stratum

corneum (SC) pH is considered as acidic in general while its precise distribution

remains to be clarified. The purpose of this study is to produce a transgenic mouse for

visualizing in vivo SC pH to enhance our understanding of the SC. We generated a

ratiometric pH biosensor with pH sensitive fluorescent protein, VenusH148G, and pH less

sensitive mCherry, expressed specifically in the uppermost layer of stratum granulosum

(SG1) by modified CRISPR/Cas9 system. Confocal microscopic analysis of living pH

imaging demonstrated that SC has at least two distinct middle-acidic and upper-neutral

layers with cross section view, rather than gradual pH changes across the layers. These

findings together indicated the dynamic and complex nature of SC in terms of pH

regulation and that the 3D in vivo pH imaging mice will provide a valuable tool to

dissect the homeostatic mechanisms of SC by pH regulation.

－22－

No.SIII-1 February 3rd

13:00-13:30

Session III Oral Presentation

High-resolution mapping of neuronal circuits with light microscopy Takeshi Imai

Laboratory for Sensory Circuit Formation, RIKEN Center for Developmental Biology

Neurons receive excitatory and inhibitory synaptic inputs from compartmentalized

structures, known as synapses. Although their geometrical distribution is crucial to

understand synaptic integration mechanisms within a neuron, its distribution at a

whole-neuron scale has not been fully established. Previously, the precise distribution of

synapses has been best studied by time-consuming serial section electron microscopy.

Due to the diffraction limit of light, conventional light microscopy could not fully

resolve the fine structure of synapses. To overcome these limitations, we recently

established a tissue clearing agent, SeeDB2, which is optimized for deep-tissue

high-resolution fluorescence imaging. Using super-resolution microscopy of

SeeDB2-cleared brain tissues, we performed a comprehensive and quantitative mapping

of dendritic spines, where excitatory synapses are formed in cortical pyramidal neurons.

We found that dendritic spines are highly enriched in a particular domain of the

dendrites, and that the biased spine distribution is established at a later stage of postnatal

development in an activity-dependent manner. Our results provide an important platform

toward our understanding of synaptic integration mechanisms in different types and

stages of dendrites in cortical pyramidal neurons. In this talk, I will also introduce our

ongoing efforts toward dense reconstruction of neuronal circuits using light microscopy.

References:

1. Ke MT, Nakai Y, Fujimoto S, Takayama R, Yoshida S, Kitajima TS, Sato M, Imai T.,

(2016) Cell Rep 14 (11), 2718-32.

2. Ke MT, Fujimoto S, Imai T., (2013) Nat Neurosci 16 (8), 1154-61.

－23－

No.SIII-2 February 3rd

13:30-14:00

Systems Biology of Mammalian Sleep/wake Cycles ~Involvement of Ca2+-Dependent Hyperpolarization in Sleep Duration in Mammals~

Hiroki R. Ueda

Laboratory for Synthetic Biology, Quantitative Biology Center, RIKEN

The detailed molecular mechanisms underlying the regulation of sleep duration in

mammals are still elusive. To address this challenge, we constructed a simple

computational model, which recapitulates the electrophysiological characteristics of the

slow-wave sleep and awake states. Comprehensive bifurcation analysis predicted that a

Ca2+-dependent hyperpolarization pathway may play a role in slow-wave sleep and

hence in the regulation of sleep duration. To experimentally validate the prediction, we

generate and analyze 26 KO mice. Here we found that impaired Ca2+-dependent K+

channels (Kcnn2 and Kcnn3), voltage-gated Ca2+ channels (Cacna1g and Cacna1h), or

Ca2+/calmodulin-dependent kinases (Camk2a and Camk2b) decrease sleep duration,

while impaired plasma membrane Ca2+ ATPase (Atp2b3) increases sleep duration.

Genetical (Nr3a) and pharmacological intervention (PCP, MK-801) and whole-brain

imaging validated that impaired NMDA receptors reduce sleep duration and directly

increase the excitability of cells. Based on these results, we propose a hypothesis that a

Ca2+-dependent hyperpolarization pathway underlies the regulation of sleep duration in

mammals.

References 1. Tatsuki et al. Neuron, 90(1) : 70–85 (2016).

2. Sunagawa et al, Cell Reports, 14(3):662-77 (2016).

3. Susaki et al. Cell, 157(3): 726–39, (2014).

4. Tainaka et al. Cell, 159(6):911-24(2014).

5. Susaki et al. Nature Protocols, 10(11):1709-27(2015).

6. Susaki and Ueda. Cell Chemical Biology, 23(1):137-57 (2016).

7. Tainaka et al. Ann. Rev. of Cell and Devel. Biol. 32: 713-741 (2016).

8. Ode et al. Mol. Cell, in press

－24－

No.SIII-3 February 3rd

14:00-14:30

CRUSING INSIDE CELLS

Atsushi Miyawaki

Laboratory for Cell Function Dynamics, RIKEN Brain Science Institute

The behavior of biochemical molecules moving around in cells makes me think of a

school of whales wandering in the ocean, captured by the Argus system on the artificial

satellite. When bringing a whale back into the sea --- with a transmitter on its dorsal fin,

every staff member hopes that it will return safely to a school of its species. A

transmitter is now minute in size, but it was not this way before. There used to be some

concern that a whale fitted with a transmitter could be given the cold shoulder and thus

ostracized by other whales for “wearing something annoying.” How is whale’s

wandering related to the tide or a shoal of small fish? What kind of interaction is there

among different species of whales? We human beings have attempted to fully

understand this fellow creature in the sea both during and since the age of whale fishing.

In a live cell imaging experiment, a luminescent probe replaces a transmitter. We

label a luminescent probe on a specific region of a biological molecule and bring it back

into a cell. We can then visualize how the biological molecule behaves in response to

external stimulation. Since luminescence is a physical phenomenon, we can extract

various kinds of information by making full use of its characteristics. For example, the

excited energy of a fluorescent molecule donor transfers to an acceptor relative to the

distance and orientation between the two fluorophores. This phenomenon can be used to

identify interaction between biological molecules or structural change in biological

molecules.

Cruising inside cells in a supermicro corps, gliding down in a microtubule like a

roller coaster, pushing our ways through a jungle of chromatin while hoisting a flag of

nuclear localization signal --- we are reminded to retain a playful and adventurous

perspective at all times. What matters is mobilizing all capabilities of science and giving

full play to our imagination. We believe that such serendipitous findings can arise out of

such a sportive mind, a frame of mind that prevails when enjoying whale-watching.

－25－

Poster Short Talks

February 2nd 16:20-17:10

2min.each 1. P-9 : Sufeng Chiang

Laboratory for Integrated Cellular Systems, Center for Integrative Medical Sciences

2. P-12 : Shingo Gibo Theoretical Biology Laboratory, Chief Scientist Laboratories

3. P-14 : Takeshi Hanami Genetic Diagnosis Technology Unit and Ultra-Sensitive Biomolecule Detection Laboratory, Center for Life Science Technologies

4. P-16 : Maxime Hebrard Laboratory for Integrated Bioinformatics, Center for Integrative Medical Sciences

5. P-19 : Yoko Ito Live Cell Super-Resolution Imaging Research Team, Center for Advanced Photonics

6. P-21 : Bogumil Kaczkowski Division of Genomic Technologies, Center for Life Science Technologies

7. P-25 : Toshimori Kitami Young Chief Investigator Program, Center for Integrative Medical Sciences

8. P-33 : Marcus Leiwe Laboratory for Sensory Circuit Formation, Center for Developmental Biology

9. P-34 : Chia-Wen Lin Laboratory of Mental Biology, Brain Science Institute

10. P-35 : Takeshi Matsui Laboratory for Skin Homeostasis, Center for Integrative Medical Sciences

11. P-38 : Nobuhiko Miyasaka

Laboratory for Neurobiology of Synapse, Brain Science Institute

－26－

February 2nd 16:20-17:10

2min.each 12. P-40 : Fumito Mori

Theoretical Biology Laboratory, Chief Scientist Laboratories

13. P-41 : Ritsuko Morita Laboratory for Tissue Microenvironment, Center for Developmental Biology

14. P-43 : Masanori Murayama Laboratory for Behavioral Neurophysiology, Brain Science Institute

15. P-44 : Nobuhiro Nakai Laboratory for Mental Biology, Brain Science Institute

16. P-47 : Noriaki Ogawa Hatsuda Quantum Hadron Physics Laboratory, Nishina Center for Accelerator-Based Science

17. P-49 : Charles Plessy Genomics Miniaturization Technology UNit, Center for Life Science Technologies

18. P-64 : Go Shioi Genetic Engineering Unit, Center for Life Science Technologies

19. P-67 : Masaru Tamura

Technology and Development Team for Mouse Phenotype Analysis, BioResource Center

20. P-68 : Todd Taylor Laboratory for Integrated Bioinformatics, Center for Integrative Medical Sciences

21. P-71 : Bo Thomsen

Theoretical Molecular Science Laboratory, Chief Scientist Laboratories

22. P-75 : Kiminori Toyooka Mass Spectrometry and Microscopy Unit, Center for Sustainable Resource Science

23. P-8 : Arno Germond

Linking gene expression with Raman spectral analysis

－27－

16 5

17 4

19 2

20 42 41 40 39 38 37 36 35 34 33 32 31 1

Elevator

43 62

44 61

46 59

47 58

Stairs

Lavatory

February 3rd09:00-10:00 (Even Numbers)

February 2nd17:40-18:40 (Odd Numbers)

21 22 23 24 2518

15 14 13 12 11 10 6

26 27 28 29 303

72 71 70 69 68 67 66 65 64 63

45 6048 49 50 51 52 53 56 5754

73 74 75 76 77 78 79 84

Sapphire

Aqua-marine

80 81 82 83

55

9 8 7

Poster Session Layout

－28－

(Alphabetical orders, in principle, some irregular arrangement due to late application, cancellation and request)

P-1

Harnessing the CRISPR/Cas9 system in mouse genetic engineering @ LARGE Takaya Abe

Takaya Abe1), Kiyonari Hiroshi1)2), Yashide Furuta1)2)

1) Genetic Engineering Team, and 2) Animal Resource Development Unit, RIKEN CLST

Laboratories for Animal Resource Development and Genetic Engineering, Center for Life

Science Technologies

P-2

Proteomic analysis of E. coli based cell-free protein synthesis Jiro Adachi

Yoshihiro Shimizu (QBiC, RIKEN)

Laboratory for cell-free protein synthesis, Quantitative Biology Center

P-3

Electrophysiological and Optogenetic Analysis of Neurons in the Mouse Claustrum Ayako Ajima

Momoko Shiozaki (RIKEN BSI), Yoshihiro Yoshihara (RIKEN BSI)

Lab for Neurobiology of Synapse, Brain Science Institute

P-4

Genetic Neuroanatomical Analysis of the Claustrum Momoko Shiozaki

Rumiko Mizuguchi(RIKEN), Sachiko Mitsui(RIKEN), Hiroki Hamanaka(RIKEN), Joshua P.

Johansen(RIKEN), Yoshihiro Yoshihara(RIKEN)

Neurobiology of Synapse, Brain Science Institute

P-5

Decoding context-dependent olfactory valence in Drosophila Laurent Badel

Kazumi Ohto (RIKEN BSI), Yoshiko Tsuchimoto (RIKEN BSI), Hokto Kazama (RIKEN BSI)

Circuit Mechanisms of Sensory Perception , Brain Science Institute

Authors and Titles for Poster Presentations

－29－

P-6

Fine mapping of mouse chromosome 10 prepulse inhibition-quantitative trait loci (PPI-QTL) points to Cdh23 Shabeesh Balan

Tetsuo Ohnishi (RIKEN-BSI), Akiko Watanabe (RIKEN-BSI), Hisako Ohba (RIKEN-BSI),

Yoshimi Iwayama (RIKEN-BSI), Yasuko Hisano (RIKEN-BSI), Takeshi Hayashi (National

Agricultural Research Centre, Tsukuba),Yoshiaki Kikkawa (Tokyo Metropolitan Institute of

Medical Science, Tokyo), Tomomi Shimogori (RIKEN-BSI), Takeo Yoshikawa (RIKEN-BSI)

Laboratory for Molecular Psychiatry, Brain Science Institute

P-7

Role of insulin/IGF signaling on body growth and adult physiology in Drosophila Kota Banzai

Laboratory for Growth Control Signaling, Center for Developmental Biology

*P-8

Linking gene expression with Raman spectral analysis Arno Germond

Taro Ichimura, Takaki Horinouchi, Hideaki Fujita, Chikara Furusawa, Tomonobu Watanabe,

(affiliation: QBIC, RIKEN)

Laboratory for Comprehensive Bioimaing

*P-9

Dissecting the regulatory mechanism and transcriptional responses triggered by the NF-kappaB and ERK signaling pathways in the antigen-stimulated B cells Sufeng Chiang

Hisaaki Shinohara (RIKEN), Huai-Kuang Tsai (Academia Sinica, TAIWAN), Mariko Okada

(RIKEN)

Laboratory for Integrated Cellular Systems, Center for Integrative Medical Sciences

P-10

A role of sumoylation in maintenance of centromeric cohesion at telophase I in meiosis Yi Ding

Masako Kaido(RIKEN)

－30－

Laboratory for Chromosome Segregation, Center for Developmental Biology

P-11

Real time 3D in vivo pH imaging of stratum corneum revealed complex morphology-based regulation in mice Yuki Furuichi

Takeshi Matsui (RIKEN), Masayuki Amagai (RIKEN, Keio)

Laboratory for Skin Homeostasis, Center for Integrative Medical Sciences

*P-12

Prediction of essential intersections between RNA methylation and circadian rhythm using models Shingo Gibo

Gen Kurosawa (RIKEN)

Theoretical Biology Laboratory, Chief Scientist Laboratories

P-13

Optimized Sequential Techniques for Marmoset Neural Circuit Mapping Mitsutoshi Hanada

Kevin Weber (RIKEN)

Laboratory for Marmoset Neural Architecture, Brain Science Institute

*P-14

Ultra-sensitive detection for miRNA by the combination of scanning single-molecule counting (SSMC) and Eprobe without amplification Takeshi Hanami

Genetic Diagnosis Technology Unit and Ultra-Sensitive Biomolecule Detection Laboratory,

Center for Life Science Technologies

P-15

Madagascar ground gecko, yet another model animal for amniotes Yuichiro Hara

Phyloinformatics Unit, Center for Life Science Technologies

－31－

*P-16

Visualization Platforms of the Laboratory for Integrated Bioinformatics Maxime Hebrard

Hidehiro Fukuyama(RIKEN), Jayson Harshbarger(RIKEN), Todd D. Taylor(RIKEN)

Laboratory for Integrated Bioinformatics, Center for Integrative Medical Sciences

P-17

Dynamic changes in replication timing mirror subnuclear compartment dynamics during differentiation Ichiro Hiratani

Hisashi Miura (RIKEN CDB) (First Author)

Laboratory for Developmental Epigenetics, Center for Developmental Biology

P-18

Dynamic transcriptional and epigenetic regulation that determines B cell fate Tomokatsu Ikawa

Tomohiro Miyai, and Junichiro Takano (YCI laboratory for Immune Regeneration)

Laboratory for Immune Regeneration, Center for Integrative Medical Sciences

*P-19

Biogenesis and maintenance of the Golgi apparatus in plant cells Yoko Ito

Tomohiro Uemura (Univ. Tokyo), Kei H Kojo (Sophia Univ.), Seiichiro Hasezawa (Univ.

Tokyo), Takashi Ueda (NIBB), Akihiko Nakano (RIKEN)

Live Cell Super-Resolution Imaging Research Team, Center for Advanced Photonics

P-20

In silico investigation of Deoxyhypusine Synthase and Deoxyhypusine Hydroxylase Matej Janezic

Akihiro Ito (RIKEN), Minoru Yoshida (RIKEN), Kam Y. J. Zhang (RIKEN)

Division of Structural and Synthetic Biology, Structural Bioinformatics Team, Center for Life

Science Technologies

*P-21

Pan cancer biomarkers from integrative analysis of transcriptome and epigenome

－32－

Bogumil Kaczkowski

Tanaka Yuji (RIKEN ACCC & PMI), Hideya Kawaji (RIKEN DGT & PMI), Yoshihide

Hayashizaki (RIKEN PMI), Alistair Forrest (Perkins Institute, Australia), Piero Carninci

(RIKEN DGT)

Division of Genomic Technologies, Center for Life Science Technologies

P-22

Fast polarization converter for microscopic analysis of protein macromolecular structures Junichi Kaneshiro

Tomonobu M. Watanabe(RIKEN QBiC), Taro Ichimura(RIKEN QBiC)

Laboratory for comprehensive bioimaging, Quantitative Biology Center

P-23

Precise analysis of microbiota dynamics using high-resolution time-series mouse fecal sampling method Seok-Won Kim

Todd D. Taylor(RIKEN)

Laboratory for Integrated Bioinformatics, Center for Integrative Medical Sciences

P-24

Development of the gene expression atlas for the marmoset brain Yoshiaki Kita

Satomi S Kikuchi(RIKEN), ChIhiro Yoshida(RIKEN), Mami U(RIKEN), Karl Windak(RIKEN),

Hirozumi Nishibe(RIKEN), Yasuhiro Go(NINS), Tomomi Shimogori(RIKEN)

Laboratory for Molecular Mechanisms of Thalamus Development, Brain Science Institute

*P-25

Dissecting the role of mitochondria in NLRP3 inflammasome activation via chemical genetics Toshimori Kitami

Uyen Thi Tran (RIKEN Center for Integrative Medical Sciences, Young Chief Investigator

Program)

Young Chief Investigator Program, Center for Integrative Medical Sciences

－33－

P-26

Neural activity measurement by the head-mounted imager developed to obtain a vast field of view in freely-moving animal Takuma Kobayashi

Hitoshi Okamoto(RIKEN)

Laboratory for Developmental Gene Regulation, Brain Science Institute

P-27

Dissection of Neural Circuitry Mediating CO2-evoked Escape Behavior in the Larval Zebrafish Tetsuya Koide

Yoshihiro Yoshihara (RIKEN)

Laboratory for Neurobiology of Synapse, Brain Science Institute

P-28

Scientific media annotation using the iCLiKVAL offline-first browser extension Naveen Kumar

Todd Taylor (RIKEN)

Laboratory for Integrated Bioinformatics, Center for Integrative Medical Sciences

P-29

A Novel Scaffold for Developing Specific or Broad-Spectrum Chitinase Inhibitors Ashutosh Kumar

Xi Jiang(Dalian University of Technology, China), Tian Liu(Dalian University of Technology,

China), Kam Y. J. Zhang(RIKEN), and Qing Yang(Dalian University of Technology, China)

Structural Bioinformatics Team, Division of Structural and Synthetic Biology,

Center for Life Science Technologies

P-31

Synthesis and transport of ciliary protein Cluap1 within a cell Lynda Lamri

Laboratoy for Organismal Patterning, Center for Developmental Biology

P-32

Extended depth-of-field microscopy for long-term imaging of synaptic plasticity.

－34－

Thomas Launey

Lab. for Synaptic Molecules of Memory Persistence, Brain Science Institute

*P-33

Spontaneous Network Activity in the Neonatal Mouse Olfactory Bulb Regulates Dendrite Pruning of Mitral Cells. Marcus Leiwe

Satoshi Fujimoto (RIKEN), Takeshi Imai (RIKEN)

Laboratory for Sensory Circuit Formation, Center for Developmental Biology

*P-34

Abnormal Development of Immune Cells Explains Brain Inflammation and GI Symptom in ASD Chia-Wen Lin

Laboratory of Mental Biology, Brain Science Institute

*P-35

Dissecting the adaptive evolution of skin by using isolated stratum granulosum cells from terrestrial vertebrates Takeshi Matsui

Masayuki Amagai (RIKEN-IMS/Keio Univ Sch of Med)

Laboratory for Skin Homeostasis, Center for Integrative Medical Sciences

P-36

Activities in the CLST Epigenome Technology Exploration Unit Aki Minoda

Epigeoome Technology Exploration Unit, Center for Life Science Technologies

P-37

Visualization and characterization of the BCR induced oscillation-like NF-κB kinetics in a B cell. Yu Miyamoto

Kazunari Iwamoto (RIKEN), Mariko Okada (RIKEN)

Laboratory for integrated cellular systems, Center for Integrative Medical Sciences

－35－

*P-38

Appetitive olfactory learning in zebrafish Nobuhiko Miyasaka

Yoshihiro Yoshihara (RIKEN)

Laboratory for Neurobiology of Synapse, Brain Science Institute

P-39

Mammalian expression of Gas Vesicle nanoparticles and its application to genetically-encoded UI/MRI multimodal contrast agent. Ryota Mizushima

Kanako Inoue (Osaka Univ.), Atsuko Iwane (RIKEN), Tomonobu Watanabe (RIKEN)

Comprehensive Bioimaging Team, Quantitative Biology Center

*P-40

Origin of cell diversity: Analysis of fixed points in Boolean networks Fumito Mori

Atsushi Mochizuki(RIKEN)

Theoretical Biology Laboratory, Chief Scientist Laboratories

*P-41

Deciphering the molecular and cellular basis of the induction of hair follicle stem cells at single cell resolution Ritsuko Morita

Ritsuko Morita1, Tetsutaro Hayashi2, Itoshi Nikaido2, Takaya Abe3, Hiroshi Kiyonari3, 4,

Yasuhide Furuta3, 4, Hironobu Fujiwara1 1 Laboratory for Tissue Microenvironment, RIKEN Center for Developmental Biology, Kobe 650–0047,

Japan

2 Bioinformatics Research Unit, RIKEN Advanced Center for Computing and Communication, Wako

351-0198, Japan

3 Animal Resource Development Unit, Division of Bio-Function Dynamics Imaging, RIKEN Center for Life

Science Technologies, Kobe 650–0047, Japan

4 Genetic Engineering Team, Division of Bio-Function Dynamics Imaging, RIKEN Center for Life Science

Technologies, Kobe 650–0047, Japan

Laboratory for Tissue Microenvironment., Center for Developmental Biology

－36－

P-42

Elucidating the role of lncRNAs in pluripotency maintenance and differentiation in human iPSCs Divya Mundackal Sivaraman

Joachim Luginbuehl (CLST RIKEN), Jordan Ramilowski(CLST RIKEN), Tsukasa

Kouno(CLST RIKEN), Suzannah Szumowski(CLST,RIKEN), Jay W Shin(CLST RIKEN)

Cell Conversion Technology Unit, Center for Life Science Technologies

*P-43

Fast-Scanning Two-Photon Microscopy for Megapixel Imaging Masanori Murayama

Keisuke Ota, BSI, Laboratory for Behavioral Neurophysiology

Laboratory for Behavioral Neurophysiology, Brain Science Institute

*P-44

Cortical Network Analysis for Behaving Mice in Virtual Reality Environment Nobuhiro Nakai

Masaaki Sato, Yasunori Hayashi, Hajime Hirase, Shigeyoshi Itohara, Toru Takumi (RIKEN

BSI)

Laboratory for Mental Biology, Brain Science Institute

P-45

Analysis of habenula neural circuit which encodes aversive expectation value Haruna Nakajo

Developmental Gene Regulation, Brain Science Institute

P-46

Respiration-Phased and Layer-Specific Operation Modes in the Olfactory Cortex Kimiya Narikiyo

Hiroyuki Manabe(Doshisha Univ.), Yoshihiro Yoshihara(RIKEN), Kensaku Mori(Univ. Tokyo)

Laboratory for Neurobiology of Synapse, Brain Science Institute

*P-47

Dynamical Pattern Selection of Growing Cellular Mosaic in Fish Retina Noriaki Ogawa

－37－

Tetsuo Hatsuda(RIKEN), Atsushi Mochizuki(RIKEN), Masashi Tachikawa(RIKEN)

Hatsuda Quantum Hadron Physics Laboratory, Nishina Center for Accelerator-Based

Science

P-48

Computational Investigation of the Role of AIOLOS Mutations in B-cell Deficiency and Familial Lymphoma Aditya Kumar Padhi

Motoi Yamashita2, Ichiro Taniuchi2 and Kam Y.J. Zhang1 （1 Structural Bioinformatics Team,

CLST, DSSB, RIKEN, Yokohama, Japan, 2 Laboratory for Transcriptional Regulation, CIMS,

RIKEN, Yokohama, Japan）

Structural Bioinformatics Team, Center for Life Science Technologies

*P-49

Single-molecule transcript assembly with unique molecular identifiers and tagmentation Charles Plessy

Genomics Miniaturization Technology Unit, Center for Life Science Technologies

P-50

Screening for novel regulators of the inactive X chromosome organization Rawin Poonperm

Ichiro Hiratani (RIKEN CDB)

Laboratory for Developmental Epigenetics, Center for Developmental Biology

P-51

Transcriptome analysis of FACS-sorted single cells with nanoCAGE Stephane Poulain

Arnaud, Ophélie ; Kato, Sachi ; Mendez, Mickaël ; Plessy, Charles (RIKEN)

Genomic Miniaturization Technology Unit, Center for Life Science Technologies

P-52

Capsule Hotel. A convenient device for imaging thousands of E. coli cells. David Geoffrey Priest

Nobuyuki Tanaka, Laboratory for Integrated Biodevice, QBiC. Yuichi Taniguchi, Head,

－38－

Laboratory for Single Cell Gene Dynamics, QBiC.

Laboratory for Single Cell Gene Dynamics, Quantitative Biology Center

P-53

Evolution of your IQ and the high price you pay for having it Pavel Prosselkov

Ryota Hashimoto (Osaka), Denis Polygalov (RIKEN), Kazutaka Ohi (Osaka), Qi Zhang

(RIKEN), Thomas J McHugh (RIKEN), Masatoshi Takeda (Osaka), Shigeyoshi Itohara

(RIKEN)

Laboratory for Behavioral Genetics, Brain Science Institute

P-54

Visualising single endocytic vesicles in neurons Morgane Rosendale

Damien Jullie (UCSF-USA), Daniel Choquet (IINS/CNRS-France), David Perrais

(IINS/CNRS-France)

Laboratory for Memory Mechanisms, Brain Science Institute

P-55

Identification of fully reprogrammed iPS cells by Raman spectroscopy Yulia Rozanova

Arnaud Germond (RIKEN), Taro Ichimura(RIKEN), Tomonobu Watanabe(RIKEN)

Laboratory for Comprehensive Bioimaging, Quantitative Biology Center

P-56

Two-photon calcium imaging using genetically-encoded calcium indicator in primate brain Osamu Sadakane

Mitsuhito Ueda(RIKEN), Akiya Watakabe(RIKEN), Hiroaki Mizukami(Jichi Medical

University), Tetsuo Yamamori(RIKEN)

Laboratory for Molecular Analysis of Higher Brain Function, Brain Science Institute

P-57

Super-multicolor Labeling and Automatic Reconstruction of Neuronal Circuits Richi Sakaguchi

－39－

Marcus Leiwe(Laboratory for Sensory Circuit Formation), Takeshi Imai(Laboratory for

Sensory Circuit Formation)

Laboratory for Sensory Circuit Formation, Center for Developmental Biology

P-58

Effects of condensin functions on chromosome organization and segregation Yuji Sakai

Masashi Tachikawa(RIKEN), Atsushi Mochizuki(RIKEN), Kazuhisa Kinoshita(RIKEN),

Tatsuya Hirano(RIKEN)

Theoretical Biology Laboratory, Chief Scientist Laboratories

P-59

Heterogeneity of pre and postsynaptic strengths in hippocampal networks during astrocyte function knockdown Abhilash Arun Sawant

Yukiko Goda, RIKEN

Lab for synaptic plasticity and connectivity, Brain Science Institute

P-60

Interactive visualization and analysis of large-scale sequencing datasets with ZENBU genome browser system Jessica Severin

Marina Lizio(RIKEN), Jayson Harshbarger(RIKEN), Hideya Kawaji(RIKEN), Michiel De

Hoon(RIKEN), Carsten Daub(Karolinska), Yoshihide Hayashizaki(RIKEN), Piero

Carninci(RIKEN), Nicolas Bertin(Cancer Science Institute Singapore), Alistair RR

Forrest(Harry Perk ins Institute)

Division of Genomic Technologies, Center for Life Science Technologies

P-61

Single molecule imaging of long noncoding RNAs Youtaro Shibayama

Divya Sivaraman (RIKEN), Jen-Chien Chang (RIKEN), Takeshi Hanami (RIKEN), Musa

Mhlanga (CSIR), Jay Shin (RIKEN)

Cell Conversion Technology Unit, Center for Life Science Technologies

－40－

P-62

Body time detection by a novel protein quantification method using mass spectrometry and cell-free protein synthesis system Yoshihiro Shimizu

Ryohei Narumi(RIKEN), Hiroki R. Ueda(RIKEN)

Laboratory for Cell-Free Protein Synthesis, Quantitative Biology Center

P-63

FANTOM6: Functional characterization of long non-coding RNAs Jay W. Shin

Cell Conversion Technology Unit, Center for Integrative Medical Sciences

*P-64

Live Imaging of Cell Behavior and Morphological Change in a Mouse Embryo during the A-P axis Formation Go Shioi

Hideharu Hoshino (RIKEN), Takaya Abe (RIKEN), Hiroshi Kiyonari (RIKEN), Kazuki Nakao

(Tokyo Univ.), Yasuhide Furuta (RIKEN), Toshihiko Fujimori (NIBB), and Shinichi Aizawa

(RIKEN)

Genetic Engineering Unit, Center for Life Science Technologies

P-65

Generation of thalamic neurons from mouse ES cells Atsushi Shiraishi

Laboratory for Cell Asymmetry, Center for Developmental Biology

P-66

Modelling human development in pluripotent stem cells generates kidney organoids with self-organizing nephrons. Minoru Takasato

Laboratory for Human Organogenesis, Center for Developmental Biology

*P-67

Visualization of non-mineralized soft tissues by X-ray: Micro-CT imaging for mouse embryonic phenotyping

－41－

Masaru Tamura

Technology and Development Team for Mouse Phenotype Analysis

BioResource Center

*P-68

Turning 'big data' into 'small data' through crowdsourced curation: integrating all types of scientific knowledge Todd D.Taylor

Naveen Kumar (RIKEN), Maxime Hebrard (RIKEN)

Laboratory for Integrated Bioinformatics, Center for Integrative Medical Sciences

P-69

Computational design of a symmetrical β-trefoil protein with galactose binding and recognition of cancer cell Daiki Terada

Arnout R. D. Voet(KU. Leuven), Kenichi Kamata(YCU), Yasuhiro Ozeki(YCU), Jeremy R.H.

Tame(YCU), and Kam Y. J. Zhang(Riken)

Structural Bioinformatics Team, Center for Life Science Technologies

P-70

Creation of an objective score for the assessment of the quality of protein design before experimental validation Pierre Guillaume Gregory THEVENET

Kam ZHANG (RIKEN)

Division of Structural and Synthetic Biology, Center for Life Science Technologies

*P-71

Visualizing Hydration Structures of Nylon-6 by Anharmonic Vibrational Calculations Bo Thomsen

Kiyoshi Yagi(RIKEN), Yuji Sugita(RIKEN)

Theoretical Molecular Science Laboratory, Chief Scientist Laboratories

P-72

Spatio-temporal dynamics of coat and adaptor proteins on the trans-Golgi network TakuroTojima

－42－

Yasuyuki Suda (Tsukuba Univ), Midori Ishii (Tokyo Univ), Kazuo Kurokawa (RIKEN), Akihiko

Nakano (RIKEN)

Live Cell Super-Resolution Imaging Reseach Team, Center for Advanced Photonics

P-73

In-vivo imaging of telencephalic neural activities in adult zebrafish in the closed-loop virtual reality environment MakioTorigoe

Tanvir Islam, Hisaya Kakinuma, Hideaki Shimazaki, Tazu Aoki, Taro Toyoizumi and Hitoshi

Okamoto (RIKEN)

Developmental Gene Regulation, Brain Science Institute

P-74

Necdin Facilitates Spine Regulation In 15q Duplication Model Mice Tsuyoshi Toya

Keita Fukumoto(RIKEN BSI, Grad. Sch. Biomed. Sci., Hiroshima Univ.), Kota

Tamada(RIKEN BSI), Shinji Tanaka(Grad. Sch. Med., Univ. Tokyo), Hidemi Misawa(Dept.

Pharmacy, Keio Univ.), Shigeo Okabe(Grad. Sch. Med., Univ. Tokyo), Toru Takumi(RIKEN

BSI, Grad. Sch. Biomed. Sci., Hiroshima Univ.), Laboratory for Mental Biology, Brain

Science Institute

*P-75

Development of correlative light and electron microscopy to observe GFP-labeled organelles embedded in resin using FE-SEM Kiminori Toyooka

Mass Spectrometry and Microscopy Unit, Center for Sustainable Resource Science

P-76

Caregiver-Infant Interactions’ Quality in Marmosets: The Role of Parental Behaviors Anna Truzzi

Kazutaka Shinozuka(RIKEN), Saori Yano-Nashimoto(RIKEN), Sayaka Shindo(RIKEN),

Atsuko Saito(RIKEN), Gianluca Esposito(University of Trento; Nanyang Technological

University), Kumi O. Kuroda(RIKEN)

Affiliative and Social Behavior Laboratory, Brain Science Institute

－43－

P-77

Mathematical model for differentiation of pre-stalk/spore cells in a social amoeba through chemical signal Kouki Uchinomiya

Yoh Iwasa (Kyushu University)

Theoretical Biology Laboratory, Other

P-78

Imaging of Hes1 Oscillation Dynamics in Mouse Embryonic Cortical Neural Stem Cells Fatma Rabia Urun

Adrian W. Moore (RIKEN)

Laboratory for Genetic Control of Neuronal Architecture, Brain Science Institute

P-79

Barp, a new neuronal regulator of voltage-gated calcium channels Rejan Vigot

Atsuko Matsunaga (RIKEN), Pascal Beguin (RIKEN), Thomas Launey (RIKEN)

Team for Synaptic Molecules of Memory Persistence, Brain Science Institute

P-80

Structure based design of peptide inhibitors against human glutaminyl cyclase Dileep Kalarickal Vijayan

Kam Zhang (RIKEN)

Structural Bioinformatics Team, Center for Life Science Technologies

P-81

An Attractive Scent of ATP: Fishes Equip a Unique Adenosine Receptor for Olfaction Noriko Wakisaka

Nobuhiko Miyasaka (RIKEN), Tetsuya Koide (RIKEN), Miwa Masuda (RIKEN), Yoshihiro

Yoshihara (RIKEN)

Laboratory for Neurobiology of Synapse, Brain Science Institute

P-82

Exploring prefrontal projection of common marmoset

－44－

Akiya Watakabe

Lab for Molecular analysis of Higher Brain Function, Brain Science Institute

P-83

Degradation Mechanism of Tbx6 during Mouse Somitogenesis Wei Zhao

Human Organogenesis, Center for Developmental Biology

－45－

Participant List Alphabetical order by last name

First name Family name Affiliation Laboratory E-mail

Mohammad Abdolrahmani BSI Neural Circuits and Behavior mohammad@benuccilab.net

Takaya Abe CLSTLaboratories for Animal Resource Developmentand Genetic Engineering abe-takaya@cdb.riken.jp P- 1

Jiro Adachi QBiC Laboratory for cell-free protein synthesis jiro.adachi@riken.jp P- 2

Ayako Ajima BSI Lab for Neurobiology of Synapse aayako@riken.jp P- 3

Takaaki Aoki QBiC Science Communication Room t_aoki@riken.jp

Laurent Badel BSILaboratory for Circuit Mechanisms of SensoryPerception laurent@brain.riken.jp P- 5

Shabeesh Balan BSI Laboratory for Molecular Psychiatry shabeeshbalan@riken.jp P- 6

Kota Banzai CDB Laboratory for Growth Control Signaling k-banzai@cdb.riken.jp P- 7

Sufeng Chiang IMS Laboratory for Integrated Cellular Systems sufeng.chiang@riken.jp P- 9

Yi Ding CDB Laboratory for Chromosome Segregation dingyi@cdb.riken.jp P- 10

Sidonia Fagarasan IMS Laboratory for Mucosal Immunity sidonia.fagarasan@riken.jp SI- 1

Emanuele Frandi BSI Lab for Neural Circuits and Behavior emanuele.frandi@riken.jp

Yuki Furuichi IMS Laboratory for Skin Homeostasis yuki.furuichi@riken.jpP-

SII-115

Arno GERMOND QBiC Laboratory for Comprehensive Bioimaing arnaud.germond@riken.jp P- 8

Shingo Gibo Ils Theoretical Biology Laboratory shingo.gibo@riken.jp P- 12

Alireza Goudarzi BSI Neural Computation and Adaptation alireza.goudarzi@riken.jp

Anne Guenther BSILab. Synaptic Molecules of MemoryPersistence anne.guenther@riken.jp

Mitsutoshi Hanada BSI Laboratory for Marmoset Neural Architecture mitsutoshi.hanada＠riken.jp P- 13

Takeshi Hanami CLSTGenetic Diagnosis Technology Unit and Ultra-Sensitive Biomolecule Detection Laboratory thanami@riken.jp P- 14

Carina Hanashima CDB Neocortical Development hanashima@cdb.riken.jp

Yuichiro Hara CLST Phyloinformatics Unit yuichiro.hara@riken.jp P- 15

Yoshie Harada Osaka UGuest SpeakerOsaka University

Yoshihide Hayashizaki PMIPreventive Medicine and Diagnosis InnovationProgram yoshihide.hayashizaki@riken.jp

Linmeng He BSI Circuit and Behavioral Physiology linmeng.he@riken.jp

Maxime Hebrard IMS Laboratory for Integrated Bioinformatics maxime.hebrard@riken.jp P- 16

Ichiro Hiratani CDB Laboratory for Developmental Epigenetics hiratani@cdb.riken.jp P- 17

Tomokatsu Ikawa IMS Laboratory for Immune Regeneration tomokatsu.ikawa@riken.jp P- 18

Takeshi Imai CDB Laboratory for Sensory Circuit Formation imai@cdb.riken.jp SIII- 1

presentation

－46－

Participant List Alphabetical order by last name

First name Family name Affiliation Laboratory E-mail presentation

Yoko Ito RAPLive Cell Super-Resolution Imaging ResearchTeam yoko.ito@riken.jp P- 19

Matej Janezic CLSTDivision of Structural and Synthetic Biology,Structural Bioinformatics Team matej.janezic@riken.jp P- 20

Worapoj Jinda Laboratory for Integrated Bioinformatics worapoj.jinda@riken.jp

Bogumil Kaczkowski CLST Division of Genomic Technologies bogumil.kaczkowski@riken.jp P- 21

Junichi Kaneshiro QBiC Laboratory for comprehensive bioimaging jkaneshiro@riken.jp P- 22

Takanori Kigawa QBiCLaboratory for Biomolecular Structure andDynamics kigawa@riken.jp

Seok-Won Kim IMS Laboratory for Integrated Bioinformatics seokwon.kim@riken.jp P- 23

Yoshiaki Kita BSILaboratory for Molecular Mechanisms ofThalamus Development kita-y@brain.riken.jp P- 24

Toshimori Kitami IMS Young Chief Investigator Program toshimori.kitami@riken.jp P- 25

Takuma Kobayashi BSI Laboratory for Developmental Gene Regulation takuma.kobayashi@riken.jp P- 26

Yasushi Kogo PMIPreventive　Medicine and DiagnosisInnovation Program yasushi.kogo@riken.jp

Tetsuya Koide BSI Laboratory for Neurobiology of Synapse tkoide@brain.riken.jpP-

SII-272

Shigeo Koyasu RIKEN riken-bsi-hd@riken.jp

Naveen Kumar IMS Laboratory for Integrated Bioinformatics naveen.kumar@riken.jp P- 28

Ashutosh Kumar CLSTStructural Bioinformatics Team, Division ofStructural and Synthetic Biology akumar@riken.jp P- 29

Vipin KUMAR QBiC Laboratory for Single Cell Gene Dynamics vipin.kumar@riken.jp

Kazuo Kurokawa RAPLive Cell Super-Resolution Imaging ResearchTeam kkurokawa@riken.jp SII- 2

Lukasz Kusmierz BSILaboratory for Neural Computation andAdaptation lukasz.kusmierz@riken.jp

Lynda Lamri CDB Laboratoy for Organismal Patterning lynda.lamri@riken.jp P- 31

Thomas Launey BSILab. for Synaptic Molecules of MemoryPersistence thomas.launey@riken.jp P- 32

Simon LECLERC QBiC single cell Gene Dynamics leclercsimon74@gmail.com

Marcus Leiwe CDB Laboratory for Sensory Circuit Formation m-leiwe@cdb.riken.jp P- 33

Chia-Wen Lin BSI Laboratory of Mental Biology chia-wen.lin@riken.jp P- 34

DianaLlerenaSchiffmacher BSI Genetic Control of Neuronal Architecture

d.llerenaschiffmacher@student.ru.nl

Takeshi Matsui IMS Laboratory for Skin Homeostasis takeshi.matsui@riken.jp P- 35

Aki Minoda CLST Epigeoome Technology Exploration Unit akiko.minoda@riken.jp P- 36

Yu Miyamoto IMS Laboratory for integrated cellular systems yu0804miyamoon@gmail.com P- 37

Nobuhiko Miyasaka BSI Laboratory for Neurobiology of Synapse miyasaka@brain.riken.jp P- 38

－47－

Participant List Alphabetical order by last name

First name Family name Affiliation Laboratory E-mail presentation

Atsushi Miyawaki BSI Cell Function Dynamics/BiotechnologicalOptics Research matsushi@brain.riken.jp SIII- 3

Ryota Mizushima QBiC Comprehensive Bioimaging Team ryota.mizushima@riken.jp P- 39

Atsushi Mochizuki ILs Theoretical Biology Laboratory mochi@riken.jp

Fumito Mori Ils Theoretical Biology Laboratory fumito.mori@riken.jp P- 40

Ritsuko Morita CDB Laboratory for Tissue Microenvironment r-morita@cdb.riken.jp P- 41

DivyaMundackalSivaraman CLST Cell Conversion Technology Unit divya.sivaraman@riken.jp P- 42

Yasuhiro Murakawa RInC RIKEN-HMC Clinical Omics Unit yasuhiro.murakawa@riken.jp

Masanori Murayama BSI Laboratory for Behavioral Neurophysiology masa_murayama@brain.riken.jp P- 43

Nobuhiro Nakai BSI Laboratory for Mental Biology nobuhiro.nakai@riken.jp P- 44

Haruna Nakajo BSI Developmental Gene Regulation haruna.nakajo@riken.jp P- 45

Akihiko Nakano RAPLive Cell Super-Resolution Imaging ResearchTeam nakano@riken.jp

Kimiya Narikiyo BSI Laboratory for Neurobiology of Synapse kimiya.narikiyo@riken.jp P- 46

Takehiro Noguchi CDB Developmental Biology Planning Office takehiro.noguchi@riken.jp

Noriaki Ogawa RNC Hatsuda Quantum Hadron Physics Laboratory noriaki@riken.jp P- 47

Kiyomi Ohno BSI Brain Science Promotion Office kiyomi.ohno@riken.jp

Mariko Okada IMS Lab for integrated cellular systems marikoh@riken.jp

Ayumi Onuki ILsInstitute Laboratories' Research PromotionOffice ayumi.onuki@riken.jp

Aditya Padhi CLST Structural Bioinformatics Team adityakumar.padhi@riken.jp P- 48

Li-Kun Phng CDB Laboratory for Vascular Morphogenesis likun.phng@riken.jp SI- 3

Charles Plessy CLST Genomics Miniaturization Technology UNit plessy@riken.jp P- 49

Rawin Poonperm CDB Laboratory for Developmental Epigenetics rawinp@cdb.riken.jp P- 50

Stephane Poulain CLST Genomic Miniaturization Technology Unit stephane.poulain@riken.jp P- 51

David Priest QBiC Laboratory for Single Cell Gene Dynamics david.priest@riken.jp P- 52

Pavel Prosselkov BSI Laboratory for Behavioral Genetics prosselkov@brain.riken.jpP-

SII-534

Morgane Rosendale BSI Laboratory for Memory Mechanisms morgane.rosendale@riken.jp P- 54

Yulia Rozanova QBiC Laboratory for Comprehensive Bioimaging yulia.riken@gmail.com P- 55

Osamu Sadakane BSILaboratory for Molecular Analysis of HigherBrain Function osamu.sadakane@riken.jp P- 56

Emi Saito BSI Brain Science Promotion Office emi.saito@riken.jp

－48－

Participant List Alphabetical order by last name

First name Family name Affiliation Laboratory E-mail presentation

Richi Sakaguchi CDB Laboratory for Sensory Circuit Formation rsakaguchi@cdb.riken.jp P- 57

Yuji Sakai RNC Theoretical Biology Laboratory ysakai@riken.jp P- 58

Lidia Sasaki ILsInstitute Laboratories Research PromotionOffice lidiassk@riken.jp

Abhilash Sawant BSI Lab for synaptic plasticity and connectivity abhilasharun.sawant@riken.jp P- 59

Jessica Severin CLST Division of Genomic Technologies jessica.severin@riken.jpP-

SII-603

Youtaro Shibayama CLST Cell Conversion Technology Unit youtaro.shibayama@riken.jp P- 61

Hideki Shigematsu CLST Protein Functional and Structural Biology Team hideki.shigematsu@riken.jp SI- 2

Yoshihiro Shimizu QBiC Laboratory for Cell-Free Protein Synthesis yshimizu@riken.jp P- 62

Tomomi Shimogori BSIMolecular Mechanisms of ThalamusDevelopment tshimogori@brain.riken.jp

Jay Shin IMS Cell Conversion Technology Unit jay.shin@riken.jp P- 63

Yoichi Shinkai ILs Cellular memory laboratory yshinkai@riken.jp

Go Shioi CLST Genetic Engineering Unit shioi@cdb.riken.jp P- 64

Momoko Shiozaki BSI Neurobiology of Synapse shiozaki.momoko@brain.riken.jp P- 4

Atsushi Shiraishi CDB Laboratory for Cell Asymmetry ashiraishi@cdb.riken.jp P- 65

Yuka Takahashi CDB Developmental Biology Planning Office yuka-t@cdb.riken.jp

Minoru Takasato CDB Laboratory for Human Organogenesis minoru.takasato@riken.jp P- 66

Toru Takumi BSI Mental Biology toru.takumi@riken.jp

Masaru Tamura BRCTechnology and Development Team for MousePhenotype Analysis masaru.tamura@riken.jp P- 67

Yuji Tanaka PMIPreventive Medicine and Applied Genomicsunit yuji.tanaka@riken.jp

Todd Taylor IMS Laboratory for Integrated Bioinformatics taylor@riken.jp P- 68

Daiki Terada CLST Structural Bioinformatics Team daiki.terada@riken.jp P- 69

Pierre THEVENET CLST Division of Structural and Synthetic Biology pierre.thevenet@riken.jp P- 70

Bo Thomsen ILs Theoretical Molecular Science Laboratory Bo.Thomsen@riken.jp P- 71

Takuro Tojima RAPLive Cell Super-Resolution Imaging ReseachTeam takuro.tojima@riken.jp P- 72

Makio Torigoe BSI Developmental Gene Regulation makio.torigoe@riken.jp P- 73

Tsuyoshi Toya BSI Laboratory for Mental Biology tsuyoshi.toya@riken.jp P- 74

Yoko Toyama CDB Developmental Biology Planning Office yoko-t@cdb.riken.jp

Kiminori Toyooka CSRS Mass Spectrometry and Microscopy Unit toyooka@riken.jp P- 75

－49－

Participant List Alphabetical order by last name

First name Family name Affiliation Laboratory E-mail presentation

Anna Truzzi BSI Affiliative and Social Behavior Laboratory anna.truzzi@riken.jp P- 76

Kouki Uchinomiya ILs Theoretical Biology Laboratory kouki.uchinomiya@riken.jp P- 77

Hiroki Ueda QBiC Laboratory for Synthetic Biology hiro@riken.jp SIII- 2

Fatma Rabia Urun BSILaboratory for Genetic Control of NeuronalArchitecture fatma.urun@riken.jp P- 78

Rejan Vigot BSITeam for Synaptic Molecules of MemoryPersistence rvigot@brain.riken.jp P- 79

Dileep Vijayan CLST Structural Bioinformatics Team dileepkvijayan@gmail.com P- 80

Noriko Wakisaka BSI Laboratory for Neurobiology of Synapse nwakisaka@brain.riken.jp P- 81

Xiaowen Wang BSI Laboratory for neuron-glia circuitry wang@brain.riken.jp

Akiya Watakabe BSILab for Molecular analysis of Higher BrainFunction akiya.watakabe@riken.jp P- 82

Motohide Yokota BSI Brain Science Promotion Office yokota@riken.jp

Charles Yokoyama BSI RIKEN Brain Science Institute cyokoyama@brain.riken.jp SI- 4

Yoshihiro Yoshihara BSI Laboratory for Neurobiology of Synapse yoshihara@brain.riken.jp

Jinze Yu BSI Circuit and Behavioral Physiology jinze.yu@riken.jp

Kam Zhang CLST Structural Bioinformatics Team kamzhang@riken.jp

WEI ZHAO CDB Human Organogenesis zhaowei@cdb.riken.jp P- 83

－50－