Richard Eberle, Ph.D. Professor Department of Veterinary Pathobiology Center for Veterinary Health Sciences Oklahoma State University-Stillwater Contact Information: E-mail: r.eberle@okstate.edu Phone: (405) 744-8169 Office: 250 McElroy Hall Stillwater, OK 74078 Education: 1974: BA, Microbiology, UCLA, Los Angeles, CA 1979: PhD, Virology, Baylor College of Medicine, Houston, TX 1980-1982: Postdoc, Cellular Immunology, University of Tennessee, Knoxville, TN Academic Appointments: 1982-1986: Assistant Research Scientist, Div Pediatrics, City of Hope National Medical Center, Duarte, CA 1986-Present: Professor, Dept Vet Pathobiology, College Veterinary Medicine, OSU, Stillwater, OK 1992-1993: Program Director, Biochemical Genetics Program, Division of Molecular & Cellular Biology, National Science Foundation. (administrative leave from OSU) 1993-1998: Associate Dean for Research, College of Veterinary Medicine, OSU, Stillwater, OK 1987-Present: Adjunct Professor, Dept Microbiology & Molecular Genetics, OSU, Stillwater, OK 2000-Present: Adjunct Professor, Dept Pathology, College of Medicine, Oklahoma University Health Science Center, Oklahoma City, OK Research Interests: Molecular biology of simian herpesviruses, anti-viral drug therapy for zoonotic monkey B virus infections, molecular basis for differential neurovirulence of simian herpesviruses Other Experience and Professional Memberships: 2001: Member, NIH Special Site Visit Committee, Wisconsin Regional Primate Research Center 2002-2007: Chairman, Scientific Advisory Committee, California Regional Primate Research Center 2002: Member, NIH Special Site Visit Committee, New England National Primate Research Center 2004-2006: Member, NIH G20/C06 Review Panel 2005: Member, NIH Special Site Visit Committee, Yerkes National Primate Research Center 2007: Special Reviewer, Hong Kong Research Grant Program 2007: Member, NIH Special Site Visit Committee, Tulane National Primate Research Center 2008: Member, NIH Special Site Visit Committee, Southwest National Primate Research Center 1997-2011: Member, Herpesvirus Study Group, International Committee on the Taxonomy of Viruses 2005-Present: Member of numerous NIH NCRR Comparative Medicine Special Emphasis Review Panels

Research Funding:

Current: 2013-2014: ACLAM Fdn, “Effective Therapy for Monkey B Virus Exposure”, Role: PI, $30,000 2013-2014: Pfizer Animal Health, “Development of Equine Herpes Myeloencephalopathy in Young Adult Horses”, Role: Co-Investigator, $100,430 2013-2018: NIH P40, "National Baboon Research Resource", Role: Co-Investigator, $6,445,411 2012-2017: NIH P40, "Development of an SPF Baboon Colony", Role: Co-Investigator, $3,858,235 Past 5 Years: 2003-2008 NIH P40, "National Baboon Research Resource", Role: Co-Investigator, $5,394,7984 2008-2013: NIH P40, "National Baboon Research Resource", Role: Co-Investigator, $6,367,083 2007-2012: NIH R24, "Development of an SPF Baboon Colony", Role: Co-Investigator, $3,517,294 2009-2011: Grayson Jockey Club, "Prevention of EHV1 Myeloencephalitis by Delayed Therapy", Role: Co- Investigator, $ 104,431 2007-2008: ACLAM, "Model-Tested Efficacy of Anti-Herpesvirus Drugs for Treatment of Monkey B Virus Infection", Role: C o-Investigator, $ 10,000 2006-2008: NIH R03, "Genetic Basis of Drug Resistant Mutants of B Virus", Role: PI, $125,000 2007-2009: Grayson Jockey Club, "Efficacy of Valacyclovir Against Neuropathogenic EHV-1", Role: Co- Investigator, $73,527 2012-2013: Pfizer Animal Health, “Prevention of EHV1 Myeloencephalitis by Vaccination”, Role: Co- Investigator, $70,589 Selected Publications:

1. Loomis-Huff, JE, R Eberle, KM Lockridge, G Rhodes & PA Barry. (2001). Immunogenicity of a DNA Vaccine Against Herpes B Virus. Vaccine. 19:4865-4873. PMID: 11535340Ohsawa, K, DH Black, R Torii, H Sato & R Eberle. (2002). Detection of a Unique Genotype of Monkey B Virus (Cercopithecine herpesvirus 1) Indigenous to Native Japanese Macaques (Macaca fuscata). Compar Med. 52:555-559. PMID: 12540170

3. Ohsawa, K, DH Black, H Sato, K Rogers & R Eberle. (2003). Sequence and Genetic Arrangement of the UL Region of the Monkey B Virus (Cercopithecine herpesvirus 1) Genome and Comparison with the UL Region of Other Primate Herpesviruses. Arch Virol. 148:989-997. PMID: 12721804

4. Rogers, KM, Ealey, KA, JW Ritchey, DH Black & R Eberle. (2003). Pathogenicity of Different Baboon Herpesvirus papio 2 Isolates is characterized by Either Extreme Neurovirulence or Complete Apathogenicity. J Virol. 77:10731-10739. PMID: 14512523

6. Payton, ME, JM d'Offay, ME Prado, DH Black, B Damania, GL White & R Eberle. (2004). Comparative transmission of multiple herpesviruses in a baboon breeding colony. J Compar Med. 54:695-704. PMID: 15679269

7. Ritchey JW, Payton ME, Eberle R. (2005). Clinicopathological characterization of monkey B virus infection in mice. Compar Pathol. Compar Med 55:246-250. ID: 15737347

8. Breshears MA, R Eberle & JW Ritchey. (2005). Temporal Progression of Viral Replication and Gross and Histological Lesions in Balb/c Mice Inoculated Epidermally with Siamirine herpesvirus 1 (SaHV-1). J Compar Pathol 133:103-113. PMID: 15964589

9. Rogers KM, RF Wolf, GL White & R Eberle. (2005). Experimental Infection of Baboons (Papio cynocephalus anubis) with Apathogenic and Neurovirulent Subtypes of Herpesvirus papio 2. Compar Med 55:425-430. PMID: 16270898

10. Leubcke E, EJ Dubovi, D Black, K Ohsawa & R Eberle. (2006). Isolation and Characterization of a Chimpanzee Alpha-Herpesvirus. J Gen Virol 87:11-19. PMID: 16361413

11. Rogers KM, JW Ritchey, M Payton, DH Black & R Eberle. (2006). Neuropathogenesis of Herpesvirus papio 2 in Mice Parallels Cercopithecine herpesvirus 1 (B virus) Infections in Humans. J Gen Virol 87:267-276. PMID: 16432011

12. Rogers, KM, D Black & R Eberle. (2007) Primary Mouse Dermal Fibroblast Cell Cultures as an in vitro Model System for the Differential Pathogenicity of Cross-Species Herpesvirus papio 2 Infections. Arch Virol. 152:543-552. PMID: 17122896

13. d'Offay, JM, R Eberle, Y Sucol, L Schoelkopf, M White, BV Valentine, GL White & NW Lerche. (2007) Transmission Dynamics of Simian T Lymphotropic Virus Type 1 (STLV-1) in a Baboon Breeding Colony: Predominance of Female-to-Female Transmission. Compar Med 57:105-114. PMID: 17348298

14. Focher, F, A Lossani, A Verri, S Spadari, A Maioli, JJ Gambino, GE Wright, R Eberle, D Black, P Medveckzy & D Shugar. (2007). Sensitivity of Monkey B Virus (Cercopithecine herpesvirus 1, BV) to Antiviral Drugs. Role of Thymidine Kinase in Antiviral Activity of Substrate Analogs and Acyclonucleosides. Antimicrob Agents Chemotherapy. Antimicrob Agents Chemother 51:2028-2034. PMID: 17438061

15. Elmore, D & R Eberle. (2008). Monkey B Virus (Cercopithecine herpesvirus 1). Compar Med 58:11-21. PMID: 19793452

16. Rogers, KM, M Deatherage, MA Breshears, D Black, JW Ritchey, M Payton, S Chapman & R

Eberle. (2009). Role of Interferon- in the Neuropathogenesis of Herpesvirus papio 2. Virol 386:280-289. PMID: 19215952

17. Black, DH, LK Maxwell & R Eberle. 2009. Characterization of a Spontaneous Drug-Resistant Mutant of Monkey B Virus (Macacine herpesvirus 1). Arch Virol 154:1495-1497. PMID: 19609635

18. Tyler, S, A Severini, D Black, M Walker & R Eberle. 2011. Structure and Sequence of the Saimiriine Herpesvirus 1 Genome. Virol. 410:181-191. DOI:10.1016/j.virol.2010.11.008

19. Ohsawa, M, R Eberle, H Sato & K Ohsawa. (2010). Re-Validation of Alternative Antigen Intended to Replace HVP2 Strain for B-Virus Diagnosis. Kyushu J Expl Anim 26:29-33.

20. Wolf, RF, R Eberle & GL White. (2010). Generation of a Specific-Pathogen-Free Baboon Colony J Am Assoc Lab Anim Sci 49:814-820. PMID: 21205446

21. Reed , DL, RW Currier, SF Walton, M Conrad, SA Sullivan, JM Carlton, TD Read, A Severini, S Tyler, R Eberle, WE Johnson, G Silvestri, IN Clarke, T Lagergård, SA Lukehart, M Unemo, WM Shafer, RP Beasley, T Bergström, P Norberg, AJ Davison, PM Sharp, BH Hahn & J Blomberg. (2011). The Evolution of Infectious Agents in Relation to Sex in Animals and Humans: Brief Discussions of Some Individual Organisms. Ann N Y Acad Sci. 1230:74-107. DOI: 10.1111/j.1749-6632.2011.06133.x. PMID: 21824167

22. d’Offay, JM, RW Fulton & R Eberle. (2013). Complete Genome Sequence of the NVSL BHV1.1 Cooper Reference Strain. Arch Virol. 158:1109-1113. doi: 10.1007/s00705-012-1574-6.

23. Fulton, RW, JM d’Offay & R Eberle. (2013). Bovine Herpesvirus-1: Comparison and Differentiation of Vaccine and Field Strains Based on Genomic Sequence Variation. Vaccine. 31:1471-1479. doi: 10.1016/j.vaccine.2013.01.013.

24. Lee, S, PE Chugh, H Shen, R Eberle & D Dittmer. (2013). Poisson Factor Models with Applications to Non-normalized MicroRNA Profiling. Bioinform 29:1105-1115. doi: 10.1093/ bioinformatics/btt091.

25. d’Offay, JM, R Eberle, RF Wolf, SD Kosanke, KR Doocy, S Ayalew, K Mansfield & GL White. Simian T-lymphotropic Virus-associated Lymphomas in 2 Naturally Infected Baboons: T-cell Clonal Expansion and Immune Response during Tumor Development. Compar Med. 63:288-294. PMID: 23759532.

26. Severini, A, SD Tyler, GA Peters, D Black & R Eberle. Genome Sequence of a Chimpanzee Herpesvirus and Its Relation to Other Primate Alpha-Herpesviruses. Arch Virol. 158:1825-1828. doi: 10.1007/s00705-013-1666-y.

27. Black, D, K Ohsawa, S Tyler, L Maxwell & R Eberle. A Single Viral Gene Determines Lethal Cross-Species Neurovirulence of Baboon Herpesvirus HVP2. Virol. In press.