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Lecture 5 Slides
Go to TA sections; challenge them!
Come to R’s and/or TAs office hours2130 Pac Hall Mon 2-3
Tue 2-3
details on course web site:
http://course.ucsd.edu/rhampton/bibc102
Please
Please
READ Glucose, Glycolysis and Krebs
But First
Please
READ First Half of The NAME GAME
READ Glucose, Glycolysis and Krebs
hppt://courses.ucsd.edu/rhampton/bibc102
fig 14-1
Things to do with glucose…
adenosine triphosphate… ATP
fig 13-11
free energies are additive!
11kg
68kg
riddle: how do you lift 11 kg? ans: lower a weight > 11kg!
In metabolism,it is all about thecoupling mechanism
fig 13-9
fig 14-2
It takesATP to make ATP!
the preparatory phase
fig 14-2
delayedgratificationin glycolyticcatabolism
the payoff phase
fig 14-3
Glucose catabolismaround the bios…
pg 532
Phosphorylation of glucose
Hexokinse: an example of induced fit
fig 6-22
Hexokinse: an example of induced fit
fig 6-22
pg 532
Isomerization of glucose 6-P
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Anomers…
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The structural simplicity of isomerase reaction
pg 532
Phosphorylation of fructose 6-P
pg 533
Cleavage of fructose 1,6 bisphosphate by aldolase
fig 14-6
Cleavage of fructose 1,6 bisphosphate by aldolase
now have two distinct3-carbonmolecules
pg 534
DHAP isomerization gives us 2 identical G3P
same chemistry, different enzyme
Isomerase action is familiar…
Equivalent carbons from different parts of glucose
fig 14-6
pg 535
A fancy redox reaction…
fig 14-7
A fancy redox reaction catalyzed by GAPDH
fig 14-7
The GAPDH active site
Enyzme-substrate complex
fig 14-7
fig 14-7
a covalentintermediatebetween acysteine Sand the substrate
The carbonmoleculegets oxidized!!
fig 14-7
fig 14-7
Phosphate as a nucleophile!!??
fig 14-7
releaseof theproduct,1,3 BPG
fig 14-7
Ready for more substrate…
fig 14-7
This is the product of interest!
pg 537
Voila! Our first ATP!
XX
Substrate level phosphorylation: the general idea
pg 537
“Isomerization” of 3PG to make 2PG
“mutase” is isomerase that moves groups
fig 14-8 (fourth)
A low level intermediate must be produced
pg 538
Making an enol form by removal of H2O
pg 538
A second substrate level phosphorylation: more ATP!
pg 538
Ta Da!!! Pyruvic acid
pg 547
LDH converts pyruvate to lactate, restoring the pool of NAD+
Glycolysis: energy without O2
anaerobic organisms
anaerobic situations
neutrophil
CEOLACANTH: the Living Fossil
Glycolysis in action: 100 mt sprint
Usain Bolt!!