Review Questions Chp 4 (KEY)[1]

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    Review Questions - Chapter 4

    Cell Structure and Function

    1.

    Be able to list the three generalizations that together constitute the cell theory.

    1. All organisms are composed of one or more cells.

    2. The cell is the smallest unit having the properties

    of life.

    3. The continuity of life arises directly from the

    growth and division of single cells.

    2.

    List and describe the three major regions that all cells have in common.

    1. A plasma membrane separates each cell from the

    environment, permits the flow of molecules

    across the membrane, and contains receptors

    that can affect the cell's activities.

    2. A nucleus ornuc leoid region localizes thehereditary material, which can be copied and

    read.

    3. The cytoplasm contains membrane systems,

    particles (including ribosomes), filaments (the

    cytoskeleton), and a semifluid substance.

    3.Cytoplasm is everything between the plasma membrane and the region of

    ____nucleus ______. [p. ]

    4. A __prokaryotic________ cell lacks a nucleus. [p. ]5. Describe the lipid bilayer arrangement for the plasma membrane.

    1. The lipid bilayer of plasma membranes forms a

    boundary between inside and outside of the cell,

    subdivides the cytoplasm into compartments, and

    regulates the entry/exit of substances. The

    hydrophobic tails face each other while the

    hydrophilic heads face outward and inward.

    2. Proteins positioned in the plasma membrane

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    serve as channels, pumps, or receptors.

    Membrane ProteinsMembrane Proteins

    Proteinpump across

    bilayer

    Proteinchannel

    across bilayer

    Protein pump

    Recognitionprotein

    Receptorprotein

    extracellularenvironment

    cytoplasm

    lipid bilayer

    Figure 4.4

    Page 57

    6.

    Describe and list the function(s) of the following membrane proteins: transport,

    receptor, recognition, and adhesion.

    Transport: Passive or active (require ATP to function. This are

    channel type or pumps

    Receptor: Modified to interact with biochemicals (hormones etc);

    these signal the cell to act in certain ways (turn on male genes in

    the case of testosterone)

    Recognition: These are modified to determine whether or not a cell

    or a biochemical in a part of the organism (allow cells to stick

    together, likes to likes, also very important n the immune system.

    Adhesion: These stick together like kind cells such as skin cells.

    7.

    Cell size is necessarily limited because its volume increases with the

    ___cube_______but surface area increases only with the __square (of the

    cell edge)___ _____. [p. ]8. Briefly describe the operating principles of light microscopes, phase-contrast

    microscopes, scanning tunneling microscopes, transmission electron microscopes,

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    and scanning electron microscopes.

    Light microscopes: Use glass lens to focus photons. Limited to

    about 2000x

    Phase-contrast microscopes: Use two contrasting light phases to to

    elucidate cell interiors with out killing the cells.

    Scanning tunneling microscopes: Most powerful types of EMs.

    These use a beam of photons and quantum physics to see down

    to the atomic realm.

    Transmission electron microscopes: Use electrons and electro-

    magnets . The latter focus the beam of electrons through a thin,

    metal stained sample and form a picture of the cells interior. Upto 100,000x and more.

    Scanning electron microscopes: These EMs reflect beams of

    electrons off the surface of targeted metal coated objects to form a

    3D image. Up to 250,000x.

    9. Briefly describe the cellular location and function of the organelles typical

    of most eukaryotic cells: nucleus, ribosomes, endoplasmic reticulum, Golgi body,various vesicles, mitochondria, and the cytoskeleton.

    Nucleus:The nucleus controls access to DNA and permits

    easier packing of DNA during cell division; it is embedded

    with in the cytoplasm.

    Ribosomes: Ribosomes , either "free" or attached to

    membranes, are the assembly sites of polypeptide chains.

    Endoplasmic reticulum: The endoplasmic reticulum (ER)

    modifies proteins and is also involved with lipid synthesis.

    Located within the cytoplasm.

    Golgi body: Golgi bodies also modify proteins, sort and ship

    proteins, and play a role in the biology of lipids for secretion

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    or internal use. Located within the cytoplasm.

    Various vesicles: Various vesicles transport, store, and digest

    various materials within the cell. Located within the

    cytoplasm.

    Mitochondria: Mitochondria have enzymes responsible for

    ATP formation. Located within the cytoplasm.

    Cytoskeleton: The cytoskeleton determines cell shape and

    internal organization; it also provides for motility. Located

    within the cytoplasm.

    10.In eukaryotic cells, __organelles_______ separate different incompatiblechemical reactions in space and time. [p. ]

    11. Describe the nature of the nuclear envelope and relate its function to its structure.

    1. The nuclear envelope consists of two lipid

    bilayers with pores.

    2. The inner surface has attachment sites for protein

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    filaments, which anchor the DNA molecules and

    keep them organized.

    3. The outer surface is studded with ribosomes.

    12.____Nucleoli______ are sites where the protein and RNA subunits of ribosomes

    are assembled. [pp. ]

    13.

    ___Chromatin_______is the cell's collection of DNA molecules and associatedproteins; a __chromosome________ is an individual DNA molecule and associated proteins. [p. ]

    14

    .

    Explain how the endoplasmic reticulum (rough and smooth types), peroxisomes, Golgi

    bodies, lysosomes, and a variety of vesicles function together as the cytomembranesystem.

    A. The endomembrane system is a series of organelles in

    which lipids are assembled and new polypeptide chains

    are modified into final proteins.

    B. Endoplasmic Reticulum1. The endoplasmic reticulum is a collection of

    interconnected tubes and flattened sacs that begins

    at the nucleus and winds its way through the

    cytoplasm.

    2. Two kinds of ER may be found in a cell:

    a. Rough ER consists of stacked, flattened sacs

    with many ribosomes attached;

    oligosaccharide groups are attached to

    polypeptides as they pass through on their wayto other organelles or to secretory vesicles.

    b. Smooth ER has no ribosomes; it is the area

    from which vesicles carrying proteins and

    lipids are budded; it also inactivates harmful

    chemicals.

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    C. Golgi Bodies

    1. A Golgi body consists of flattened sacs--resembling

    a stack of pancakes--whose edges break away as

    secretory vesicles.

    2. Here proteins and lipids undergo final processing,

    sorting, and packaging.

    D. A Variety of Vesicles

    1. Lysosomes are vesicles that bud from Golgi bodies;

    they carry powerful enzymes that can digest the

    contents of other vesicles, worn-out cell parts, or

    bacteria and foreign particles.

    2. Peroxisomes are small vesicles that contain

    enzymes using oxygen to degrade fatty acids and

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    amino acids, forming a harmful byproduct, hydrogen

    peroxide, which is then converted to water.

    15

    .

    Describe the function of exocytic vesicles; also describe the function of endocytic

    vesicles.Exocytic vesicles export materials out of cells via the cell membrane.

    These vesicles actually fuse with the cell membrane to release the

    material.

    Endocytic vesicles move packaged goods (various biochemicals)through out the cytoplasm.

    16

    .__Lysosomes________ are organelles of intracellular digestion that bud from Golgimembranes of animal cells and some fungal cells. [p. ]

    17.

    Define and describe the function of peroxisomes.

    Peroxisomes are small, membrane bound vesicles that

    contain enzymes using oxygen to degrade fatty acids

    and amino acids, forming a harmful byproduct,

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    hydrogen peroxide, which is then converted to water.

    18

    .

    Within __mitochondria________, energy stored in organic molecules is releasedby enzymes and used to form many ATP molecules in the presence of oxygen.

    19

    .

    Describe the detailed structure of the chloroplast, the site of photosynthesis (include

    grana and stroma).

    Chloroplasts are oval or disk shaped, bounded by adouble membrane, and are critical to the process of

    photosynthesis.

    a. In the stacked disks (grana), pigments and

    enzymes trap sunlight energy to form ATP.

    b. Sugars are formed in the fluid substance

    (stroma) surrounding the stacks.

    c. Pigments such as chlorophyll (green) confer

    distinctive colors to the chloroplasts.

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    20

    .

    Give the general function of the following plant organelles: chloroplasts, chromoplasts,amyloplasts, and the central vacuole.

    Chloroplasts: Site of photosynthesis

    Chromoplasts store red and brown pigments that give color topetals, fruits, and roots.

    Colorless amyloplasts store starch granules.

    21.

    Elements of the __cytoskeleton________give eukaryotic cells their internalorganization, overall shape, and capacity to move. [p. ]

    22

    .

    List the three major structural elements of the cytoskeleton and give the general

    function of each.

    1. The main components are microtubules,

    microfilaments, and intermediate filaments--all

    assembled from protein subunits.

    2. Some portions are transient, such as the "spindle"

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    microtubules used in chromosome movementduring cell division; others are permanent, such as

    microfilaments operational in muscle

    contraction. Intermediate filaments are alsopermanent structures in some animal cells. They

    provide structure for these cells.

    23

    .

    Describe the general functions of motor proteins and crosslinking proteins.

    Motor proteins: Type of protein (e.g., myosin) attached tomicrofilaments and microtubules; used in cell movements(e.g., contraction).

    Cross linking proteins: Proteins that join various cytoskeletal

    elements to form mesh-like structures such as the cell

    cortex.

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    KinesinKinesin

    http://fondazione-elba.org/nsito/abstract/37.htm

    24

    .Amoeba proteus, a soft-bodied protistan, crawls on __pseudopods________. [p. ]

    25.

    Both cilia and flagella have an internal microtubule arrangement called the "______9

    + 2_______" array. [p. ]

    26

    .

    A centriole remains at the base of a completed microtubule-producing center where it

    is often called a _basal body___________________. [p. ]27

    .

    Distinguish a primary cell wall from a secondary cell wall in leafy plants.

    Primary cell wall: A thin, flexible plant cell wall of cellulose,polysaccharides, and glycoproteins; allows growing cells todivide or change shape.

    Secondary cell wall: Of older plant cells no longer growing butin need of structural support, a wall on the inner surface of theprimary wall. Contains lignin in older cells of woody plants.

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    28

    .

    Describe the location and function of plasmodesmata.

    In plants tiny channels called plasmodesmata cross

    the adjacent primary walls and connect the cytoplasm

    of adjacent cells.

    29

    .

    ___Tight_______junctions link the cells of epithelial tissues;

    __adhering________junctions join cells in tissues of the skin, heart, and otherorgans subjected to stretching; __gap________ junctions link the cytoplasm ofneighboring cells. [p ]

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