Escobedo LN, López C, Méndez MV.

Expression of integrin B1 and vinculin as cellular adhesion marker in materials for retrograde obturation

Aim: To assess the expression of integrin B1 and vinculin as cellular adhesion marker of 3 materials for retrograde obturation at 1 hour, 5 days and 10 days.

Introduction: Periapical surgery, which makes up 3-10% of all procedures in endodontic practice1, has a success rate of 85-94% corresponding to apicoectomy2,3. After the root resection and retrograde cavity preparation, a material of retroobturation is placed. The objective is to provide an apical seal that prevents the migration of antigens from the root canal to the periradicular tissues or viceversa4.

These materials will be in close contact with the surrounding tissues. Thus, the biocompatibility of the material is of crucial importance to avoid adverse reactions in the periapical tissues5.

Materials and methods: Cells were obtained from an oral mucus biopsy of a healthy patient with the previous informed consent. Materials were prepared according to the manufacturers’ instructions under aseptic conditions. The specimens were kept in an incubator for the length of time set by the manufacturer (15 minutes for the IRM and Super EBA and 4 hours for the MTA). The specimens were sterilized under ultraviolet light. 1 ml of cell growth medium with fibroblast (5x107) was added to them and they were placed in an incubator at 37° C for 1 hour, 5 days and 10 days. A control group was used during the process. After the period of observation was finished, the samples were evaluated with the live/dead kit and phalloidin. The expression of adhesion proteins integrin-β1 and vinculin was also evaluated. The cellular adhesions were studied due to the presence or absence of filopodia and lamellipodia.

Conclusion: The statistic analysis was performed through the HSD Tukey and ANOVA tests. The MTA group showed the highest levels of protein expression and focal adhesion, followed by IRM and Super EBA. Also, MTA had a higher expression of integrin-β1 and vinculin, followed by IRM and EBA. These last two showed no statistic difference of significance. IRM showed the lowest focal adhesion levels, whilst the MTA group had the highest rate. This result suggests good biocompatibility for MTA as it showed the highest level of adhesion.

Focal adhesions in arbitrary units/ Days (CAMBIAR POR FAVOR)

Días

Ad

he

sio

ne

s F

oca

les e

n u

nid

ad

es a

rbitra

ria

s

1 5 10

02

04

06

08

0

Días

Exp

resió

n d

e B

1 in

teg

rin

a e

n u

nid

ad

es a

rbitra

ria

s

1 5 10

01

02

03

04

05

06

0

ControlMTASuperEBAIRM

EXPLICACIÓN DE LA TABLA ANTERIOR: Cell viability expression in control and experimental groups at 1 hour, 5 days and 10 days.

Images of fibroblasts taken with a confocal microscope (Leica DMI400B). Image 6. Control group 10 days phalloidin and Sytox. Image 7. MTA, 10 days without phalloidin and Sytox. Image 8. Super EBA, 10 days without phalloidin and Sytox. Image 9. IRM, 10 days without phalloidin and Sytox.

1) Johnson BR et al. Periradicular Surgery. In: Cohen S, Hargreaves KM, editors.

Pathways of the Pulp. 9th ed. Missouri: Mosby; 2006. pp. 724–85.

2) Sumi Y et al. Ultrasonic root-end preparation: clinical and radiographic evaluation

of results. J Oral Maxillofac Surg 1996; 54: 590-3.

3) Testori T et al. Success and failure in periradicular surgery. A retrospective analysis.

Oral Surg Oral Med Oral Pathol 1999; 87: 493-8.

4) Torabinejad M et al. Antibacterial effects of some root end filling materials. J

Endod, Vol 21, No 8, 1995.

5) Buddy D. Ratner ¨Biomaterials Science” 2ª Edition, Ed. Academic Press Inc. 1996.

6) Friedman S. The Prognosis and expected outcome of apical surgery. Endodontic

Topics 2005, 11, 219-262.(Courtesy of Dr Richard Rubinstein, Farmington Hills, MI,

USA.)

EN LA IMAGEN DE LA APICECTOMÍA PONER REFERENCIA 6