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DHRUV R PATEL, B.S., M.S.
3250 John F Kennedy Blvd, Floor 2
Jersey City, NJ 07306
(732)-599-4254
Self-motivated, results driven research associate versed in state-of-the art
technologies, methodologies, and instrumentation used in today’s modern
molecular and cellular biology laboratories.
Emphasis on cell based assays to investigate potential candidates selected for pre clinical or clinical
studies. Advanced skills in cloning along with tissue culturing and a special interest in analyzing and
translating novel biological moieties into diagnostic/therapeutic entities.
Highly competent, personable associate scientist, able to work with diverse groups of people.
Core Expertise and Lab Skills
� Molecular and Cellular Biology Techniques
o Cloning
o PCR and RT PCR
o DNA Isolation
o Electroporation
� Cell and Tissue Culturing
o Transient Transfection
o Viral Infection
� Cell Based and In Vitro Assay Development
o ELISA Assay
o Immuno Fluorescence Assay
� Protein Purification
o Affinity Chromatography
o Size Exclusion Chromatography
� Protein and Antibody characterization
o SDS PAGE
o Western Blotting
� Reagent Repository and Database
Development
� Software based Applications
o NEB Cutter, MAC Vector, BLASTn,
BLASTx, MS Office Suite
� Animal Handling
� Institutional Regulatory Compliance � Multiple Leadership Roles
Education 2009 - 2011 St John’s University, Queen NY M.S., Biological & Pharmaceutical Biotechnology 2004 - 2008 Rajiv Gandhi University of B.S., Pharmaceutical Sciences Health Sciences, Karnataka, INDIA
Research & Experience Oct 2010 – Sept 2011 Post-graduate Research Intern Regeneron Pharmaceuticals, NY
� Conducted discovery research to identify and validate new targets for Obesity and Metabolic
Disease group.
� Implemented and populated a repository for molecular biology reagents that include, plasmid
clones, maxipreps and glycerol stocks of DNA clones and sequencing primers.
� Expedite the tracking for every reagent by creating excel based database which also
contained relevant information for each of the reagent.
� Manufactured 300-400 Plasmid clones using basic molecular lab techniques.
� Constructed plasmid clones that contained target gene of interest by following regular
cloning strategies.
� Amplified cloned plasmid DNA through bacterial transformation and performed high
throughput DNA isolation using Vacuum manifold protocol and 96well format DNA
isolation.
� Optimized the screening of positive clones through restriction digestion analysis and
DNA sequencing.
� Analyzed protein expression after transfecting them in mammalian cell system, through
immunochemical assays like SDS PAGE and western blotting.
� Characterized antibodies and used them in developing functional cell based assays to screen
activity and localization for new targets.
May 2010 - Aug 2010 Student Assistant St John’s University, NY
� Responsible for tutoring Bio Labs to undergraduate students, grading quizzes and exams.
� Assisted fellow teaching assistants in demonstrating lab skills to understand basic mammalian
anatomy using fetal pig as a model.
� Awarded Student Assistantship by Department of Biological Sciences, St John’s University.
Jan 2010 – May 2010 Graduate Special Research St John’s University, NY
“Induction and Analysis of protein expression by truncated region of eIF4GI in E.coli BL21 DE3 cells"
• Investigated the truncated version of eukaryotic Initiation Factor eIF4GI by cloning it into vector
pET duet I and thereafter analyzed its protein expression in E.coli BL21 DE3 cells.
Sept 2009 – Dec 2009 Academic Research Project St John’s University, NY
“Identification of unknown cDNA by inducing and analyzing protein expression in E.coli BL21 DE3 cells
as ACMPK gene that encodes for Ca2+/calmodulin (CaM) – dependent multi functional protein kinase”
• Characterized and identified the unknown cloned cDNA through restriction enzyme digestion as ACMPK
gene.
• Amplified the cloned cDNA using E.coli DH5α cells and after inducing protein expression through E.coli BL21 cells isolated and purified the corresponding CaMK protein by Ni Affinity Column
Chromatography.
May 2009 – Dec 2009 Undergraduate Academic Project KLESCP, Karnataka, INDIA
“Bioassay of Histamine and Acetylcholine using isolated guinea pig ileum"
• Studied agonistic and antagonistic effect of drugs using isolated guinea pig ileum preparation.
• Conducted laboratory animal handling like mice, rat and guinea pig.
• Administered drugs and placebo in mice and rat through oral, intravenous and intra peritoneal routes.
• Recorded the dose response curve of Acetylcholine using rectus abdominis muscle preparation.
March 2009 – Apr 2009 Industrial Trainee Hamax Pharmaceuticals, INDIA
• Worked in Production and Quality Assurance Department and performed pre-formulation and
dissolution studies.
• Underwent extensive training on various aspects of Quality Compliance, Regulatory and Clinical
Practices (FDA, ICH) required producing an effective, safe, compliant and readying- to- market product.
• Assisted in preparing quality plans (GMP, GLP), reviewing validation protocol (SOP) and auditing
procedures.
Professional & Leadership Highlights
Sept 2009-May 2010 Event Management Staff St John’s University, NY
� Coordinated management policy, documentation, and training ensuring compliance with:
� University event management regulations.
� Campus building and safety regulations.
� Managed inventory and ordering within budgetary constraints.
� Trained and mentored team of undergraduate and junior graduate student as event
management staff.
Sept 2004- Dec 2008 Student Government Organization KLESCP, Karnataka, INDIA Elite member (2006-2008) / Graduate Student Council Delegate (2004-2006)
� Represented students in Graduate Student Council.
� Organized annual academic extracurricular events for undergraduate and graduate students
during tenure like:
� 2nd International Symposium of Drug Discovery and Process Research (DDPR).
� Blood Donation Camp and Tree Plantation Project in JNMC campus, Karnataka India.
------- REFERENCES: available when asked for -------
