RESEARCH PROJECT PROPOSAL FORM - II 2012-2013PROGRESS REPORT OF RESEARCH PROJECT

PART I GENERAL INFORMATION

600 Project Code:

6001 Institute code No.

6002 Project code No CI 3.8

601 Name of Institute and Division

6011 Name & Address of Institute: INDIAN GRASSLAND AND FODDERRESEARCH INSTITUTE, JHANSI -284 003

6012 Name of Division: Crop Improvement Division IGFRI, Jhansi.

6013 Location of Project: Crop Improvement Division IGFRI, Jhansi.

602 Project Title: "Genetic Improvement in fodder Cowpea Vigna unguiculatawith special reference to resistance to pests."

603 Priority Area

6031 Research Approach: Basic and Applied Research

604 Specific Area:

605 Duration:

6051 Date of start:

5 Years

2009

6052 Likely date of completion: 2014

6053 Period for which report submitted 2012 - 2013

606 Total cost of the project:

607 Summary Achievements:Hybridization:

Crosses attempted of identified pest tolerant lines: IC 438373, IC 438372, EC 548976,EC 549012 and wild sps V. unguiculata cv.pubescence EC 548865 with released varietyBL-2 (susceptible).Three crosses materialized i.e.

• . BL-2 X IC 438373(one pod)• BL-2 X IC 5490 I2(two pods)• BI-2 X EC 548865(wild sps) one pod.

Nucleus seed production:Seeds collected of released varieties:

.:. BL- I (I OKg)

.:. BL-2 (8Kg)

.:. Kohinoor (1Okg)

.:. EC- 42 I6 (8kg)

Laboratory validation of Semi looper (PIusia nigrisigna Wlk.) damage:Relative resistance of cowpea (Vigna unguiculata (L.) Walp.) genotypes to semilooper(Plusia nigrisigna Wlk.) were studied under laboratory conditions. Field resistantgenotype viz. IC-438373, IC-438372 and EC-549012 along with a susceptible/standardcheck BL-2 were further confirmed under artificial infestation conditions in laboratoryand identified as resistant donors aligned with semilooper (Plusia nigrisigna Wlk.)incidence. Laboratory screening for resistar.ce was done by using plant leaf and allowingforced feeding by semilooper, Plusia nigrisigna larvae. Average leaf damage under no-choice/forced feeding was recorded to be 13.80 to 19.00% in IC-438373, 14:50% to 17.50% in IC-438372 and 16.66% to 20.00 % in EC-549012 while 25.00% to 39.00% insusceptible check BL-2.

Conduction & reporting of Data of AICRP Forage trials carried out in Kharif2012~ A VT -1Fodder Cowpea

GFY/plot in q/ha: Entry AVTC-I-5 (335.42) was highest yielder, Trial mean (308.68)DMY/Plot in q/ha: Entry AVTC-I-5 (62.20) was highest yielder, Trial mean (54.51)

~ AVT -2Fodder CowpeaGFY/plot in q/ha: Entry AVTC-2-7 (297.92) was highest yielder, Trial mean (284. I7)DMY/Plot in q/ha: Entry AVTC-2-7 (49.65) was highest yielder, Trial mean (47.41)

~ IVTC Fodder CowpeaGFY/plot in q/ha: Entry IVTC-2 (366.67) was highest yielder, Trial mean (306.25)DMY/Plot in q/ha: Entry IVTC-2 (61.30) was highest yielder, Trial mean (50.64)

~ A VT-2 Soybean (Seed)SeedYield/plot in q/ha: Entry AVTS-2-4 (8.25) was highest yielder, Trial mean (6.23)

~ A VT-2Fodder Cowpea(Seed)SeedYield/plot in q/ha: Entry AVTC-2-7 (7.92) was highest yielder, Trial mean (6.50)

Elite lines Multiplication:• 500gm seeds collected ofIL-I063, IC-438372, IC-438373, EC-549012 each .•. IL-I 177 (10kg), RR-3 (7 kg)

Carrying forward of hybrids for higher biomass• BL-2 X EC4216• EC4216 X BL-2• UPC5287 X BL-2

F3 generation breeding material was screened for pest tolerance but none of the linesappeared tolerant.

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Long term storage:Seeds of 23 cowpea accessions sent to NBPGR, New Delhi for long term storageand issue ofle No. . .

S.No. Accession No. Other-Id Breeder line No.1. IC-0596721 IGFRI-05-68 IL-1155-B2. IC-0596722 IGFRI-05-71 IL-11773. IC-0596723 IGFRI-05-72 IL-II77-A4. IC-0596724 IGFRI-05-73 IL-II77-B5. IC-0596725 IGFRI-05-79 IL-3152-16. IC-0596726 IGFRl-05-85 IL-31777. IC-0596727 IGFRI-012-6 RR-3

PART II INVESTIGATOR PROFILE

610 Principal Investigator6101 Name: Dr. (Mrs) Gitanjali Sahay6102 Designation: Sr. Scientist6103 Division/Section: Crop Improvement Division.6104 Location: Jhansi.6105 Institute Address: IGFRI, Jhansi-284 003

611 Co - Investigator6111 Name: Dr. N.K.Shah6112 Designation: Principal Scientist6113 Division/Section: Crop Improvement Division.6114 Location: Jhansi6115 Institute Address: IGFRI, Jhansi-284 003

612 Co - Investigator6121 Name: Sri. R.B.Bhaskar6122 Designation: Senior Scientist6123 Division/Section: Crop Improvement Division.6124 Location: Jhansi6125 Institute Address: IGFRI, Jhansi-284 003

613 Co - Investigator6131 Name: Kumar Durgesh6132 Designation: Scientist6133 Division/Section: Crop Improvement Division6134 Location: Jhansi6135 Institute Address: IGFRI, Jhansi-284 003

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PART III: - TECHNICAL DETAILS

620 Introduction and objectives(Problem Identification)

6201

~

Immediate objectives:

To carry forward the recommendations of concluded programme i.e.~ Utilization ofthe donor sources in breeding.~ Registration of pest resistant lines.~ Carrying forward of promising selections F5 generation.

~ Collection and evaluation of new germplasm~ Characterisation of newly introduced material, selected lines for registration~ Identification and evaluation of erect type of material

6202 Long Term Objectives:

1) To synthesize improved high yielding varieties with major stress to root-rotdisease and insect pests.

2) Selection of elite Iines for AICRPF.3) Registration and Documentation of germplasm entries lines.

621 Specific objectives for the year

Technical Programme:

• Crossing programme• Carrying forward of hybrids• Elite lines Multiplication• Nucleus seed production• AICRP Trials, 2012-13

6211 Organization of work elements (for each objective and participatinginvestigator giving man-month involved).Methodology:

6212 Man month's involvement of component Project workers for the specified year.

622 Progress 2012-2013:6221 A hi t . t f ta t fi d f hA ..c ievemen SIn erms 0 rge s ixe oreac ctivity

Targets Achievements

Hybridization: Crosses attempted of identified pest tolerant lines: IC 438373, IC 438372,EC 548976, EC 549012 and wild sps V unguiculata cv.pubescenceEC 548865 with released variety BL-2 (susceptible).

Three crosses materialized i.e.• BL-2 X IC 438373(one pod)• BL-2 X IC 549012(two pods)• BI-2 X EC 548865(wild sps) one pod.

Elite lines Multiplication • 500gm seeds collected of IL-I 063, IC-438372, IC-438373,EC-5490 12 each.

• IL-ll77 (10kg), RR-3 (7 kg)Conduction and reporting ~ AVT -/ Fodder Cowpea

of Data of AICRP Forage GFY/plot in q/ha: Entry AVTC-I-5 (335.42) was highest yielder, Trial mean (308.68)DMY/Plot in q/ha: Entry AVTC-I-5 (62.20) was hizhest yielder, Trial mean (54.51)

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Crops trials carried out in ~ A VT -2Fodder Cowpea

Kharif2012 GFY/plot in q/ha: Entry AVTC-2-7 (297.92) was highest yielder, Trial mean (284.17)DMYIPlot in q/ha: Entry AVTC-2-7 (49.65) was highest yielder, Trial mean (47.41)

~ IVTC Fodder CowpeaGFY/plot in q/ha: Entry IVTC-2 (366.67) was highest yielder, Trial mean (306.25)DM"Y/Plot in q/ha: Entry IVTC-2 (61.30) was highest yielder, Trial mean (50.64)

~ AVT-2 Soybean (Seed)SeedYieldiplot in q/ha: Entry AVTS-2-4 (8.25) was highest yielder, Trial mean (6.23)

~ A VT-2Fodder Cowpea(Seed)SeedYieldiplot in q/ha: Entry AVTC-2-7 (7.92) was highest yielder, Trial mean (6.50)

Nucleus seed production: Seeds collected of released varieties:.:. BL-l (lOKg).:. BL-2 (8Kg).:. Kohinoor (I Okg ).:. EC- 4216 (8kg)

Carrying forward of • BL-2 X EC4216hybrids for higheJ' • EC4216 X BL-2biomass • UPC5287 X BL-2

F3 generation breeding material was screened for pest tolerance butnone of the lines appeared tolerant.

Laboratory validation of Relative resistance of cowpea (Vigna unguiculata (L.) Walp.)Semi looper (Plusia genotypes to semilooper (Plusia nigrisigna Wlk.) were studied undernigrisigna Wlk.) damage: laboratory conditions. Field resistant genotype viz. IC-4-38373, IC-

438372 and EC-5490 12 along with a susceptible/standard check BL-2were further confirmed under artificial infestation conditions inlaboratory and identified as resistant donors aligned with semi looper(Plusia nigrisigna Wlk.) incidence. Laboratory screening for resistance

, was done by using plant leaf and allowing forced feeding bysemi looper, Plusia nigrisigna larvae. Average leaf damage under no-choice/forced feeding was recorded to be 13.80 to 19.00% in IC-438373, 14.50% to 17.50 % in IC-438372 and 16.66% to 20.00 % inEC-5490 12 while 25.00% to 39.00% in susceptible check BL-2.

Long term storage: Seeds of 23 cowpea accessions sent to NBPGR, New Delhi for long termstorage and issue ofIC No.

S.No. Accession No, Other-Id Breeder line No.1. IC-0596721 IGFRI-05-68 IL-ll55-B2. IC-0596722 IGFRI-05-71 IL-II773. IC-0596723 IGFRI-05-72 IL-ll77-A4. IC-0596724 IGFRI-05-73 IL-ll77-B5. IC-0596725 JGFRI-05-79 IL-3152-16. IC-0596726 JGFRI-05-85 IL-31777. IC-0596727 IGFRI-O 12-6 RR-3

6222 Questions - Answer:

6223 Process/Product/Technology developed during the year.Hybrids of identified pest tolerant lines: IC 438373, IC 438372, EC 548976,EC 549012 and wild sps V. unguiculata cv.pubescence EC 548865 withreleased variety BL-2 (susceptible) materialised.i.e.

• BL-2 X IC 438373(one pod)• BL-2 X IC 5490 12(two pods)• BI-2 X EC 548865(wild sps) one pod.(for

exploring possibility of multi cut)

•

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• Nucleus Seed production of released varieties of Cowpea Bundellobia-l, Bundellobia-2, Kohinoor and EC 4216

• Long term storage:Seeds of recently identified cowpea accession (IL-ll77) for varietal release asBundel lobia-4 for North Eastern zone sent to NBPGR, New Delhi for longterm storage and issue of IC No. (IC-0596722).

6224 Utility of results obtained so far.

623 Publications:

6231 Research paper:.:. Sahay G, Malaviya DR, Singh UP. 2013. Morphological traits association with

fodder and seed yield in Vigna unguiculata (L.) Journal of environmental biology,Vol 34, 139-145.

Popular articles:.:. G.Sahay, N.K.Shah, R.B.Bhaskar, Uttam poshtik chara hetu lobiya ki ek nai kisam

Bundel lobia-t. Submitted to Chara patrika (In press)

News letter:.:. Gitanjali Sahay, N K Shah, R B Bhaskar and K Durgesh, Bundel lobia-A, a new

variety of fodder cowpea Vigna unguiculata (l)-valp for North Eastern ZoneSubmitted to IGFRl Newsletter (Sept-Dee 2012)(In press)

Abstracts:':. National Symposium on Sustainble production of torage from arable and non-

arable land and it's utilization during 2-3 November ~'GI2. RMSI, IGFRI, Jhansi.Sahay G, Durgesh K, Shah NK and Bhaskar RB 201. . Heterosis for fodder andseed yield in crosses involvingthree Cowpea varieties.pu 31 .

Compilation/documentation:.:. Compiled "50 years of research in Cowpea Breeding at j,JFRI Jhansi" for the

book entitled, "50 Years of Forage Research" released on the Institute'sfoundation day 2012 .

•:. Compiled the chapters entitled, "Dual purpose .legume crops for nutritionalsecurity" and "Improving fodder legumes for sustaining productivity underclimate change" for the training compendium of Model training Coursesorganized by Crop Improvement Division, IGFRI Jhansi on November 19th _26th

,

2012 and 041h -II th February 2013 respectively.

Reports:

Submitted• Data of AICRP trials 2012,• Annual report• Six monthly report submitted• Six monthly( HYPM),• Quarterly and monthly reports.

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6232 Seminar and workshops (relevant to the project) in which the scientist have

participated.

• Attended the Annual group meeting of AICRP on Forage crops (Rabi- 2012)

held at IGFRI Jhansi from 14thto is" September 2012.

• Attended National Symposium on Sustainble production of forage from arable and

non-arable land and it's utilization during 2-3 November 2012. RMSI, IGFRI,

Jhansi.

• Participated as resource person in Model training course "Fodder Crops for

Climate Resilient Agriculture", sponsored by Directorate of Extension, Ministry

of Agriculture (GOI), New Delhi from 04th -11th February 2013, and delivered a

lecture on the topic "Improving fodder legumes for sustaining productivity under

Climate change" on 07.02.13.

• Participated as resource person in Model training course on the topic,"Dual

Purpose Fodder Crops and Trees for Nutritional & Food Security," sponsored by

Directorate of Extension, Ministry of Agriculture (GOI), New Delhi from

November 19th -26th, 2012 and delivered a lecture on the topic "Dual purpose

legume crops for nutritional security" on 20.11.2012.

• Participated in "Mahila Krishi Goshthi "organized by Social Science Division,

IGFRI, Jhansi on 8th March 2013 at Village, Domagore, Jhansi.

624 Infrastructures facilities developed:

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Signature ofthe Principal Investigator: (L,--\ \ J..; ~~~o,b·l2:.

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3.~l~Signature & Comments of the Head of the 0' " "~ + "'~<A< ~ .IViSlon

Co- Investigators

DIRECTOR

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Signature & Comments of the 0"irector

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