Research Highlights

Biomedical and Pharmaceutical Materials (BPM)

Biomedical and Pharmaceutical Materials (BPM)

• Biomaterials for drug delivery, medical device coatings, and tissue engineering

• Drug/medical device combinations, characterization of drug/materials interactions

• Cell-based fabrication of bioartificial tissues

• Novel tissue mechanical testing and analysis methods

Investigator Department Expertise

Ron Siegel* Phm1/BME2 hydrogels, drug delivery systems, microfabrication

Effi Kokkoli CEMS3 bioadhesion and drug targeting

Jayanth Panyam Phm multifunctional nanodelivery vehicles

Wei Shen BME bioactive materials

Calvin Sun Phm drug crystal and particle engineering

Raj Suryanarayanan Phm solid state properties of drugs, stability of drug/biomaterial formulations

Bob Tranquillo BME/CEMS fabrication and characterization of bioartificial cardiovascular replacement tissues

Chun Wang BME bio-molecular materials, polymer-based DNA and drug delivery, protein-based tissue

scaffolds*Program Leader (Email:siege017@umn.edu)

Affiliated Investigators: Chris Macosko,3 Marc Hillmyer,4 Theresa Reineke,4 Tom Hoye.4

1Pharmaceutics; 2Biomedical Engineering; 3Chemical Engineering and Materials Science, 4Chemistry

Inert Biodegradable Surfaces with “Artificial Mucus”

Hydrophobic graft (PCL)Hydrophilic core (HA)

TCP PCL

PCL/(1% HA-g-PCL) PCL/(3% HA-g-PCL)

0

20

40

60

80

100

120

TCP PCL PCL/1%HA-g-PCL

PCL/3%HA-g-PCL

Cel

l cou

nt

1 week4 weeks

Medical device surface

Biodegradable matrix

Medical device surface

Active nanostructured

particles

Biocompatible surface coating

Medical device surface

Biodegradable matrix

Medical device surface

Active nanostructured

particles

Biocompatible surface coating

(PCL)

W. Wang, R. A. Siegel, and C. Wang, ACS Biomaterials Science & Engineering, 2, 180-187 (2016)

Chun Wang, wangx105@umn.edu, 612-626-3990, www.tc.umn.edu/~wangx504/index.htmlRon Siegel, siege017@umn.edu, 612-624-6164, www.pharmacy.umn.edu/bio/pharmacy-faculty-a-z/ronald-siegel

(1) Addition of a cell mixture on the capturesubstrate.

(2) Capture of target cells.(3) Washing of non-target cells.(4) Release of captured cells using the

molecular trigger B-PEG.

(4)(2) (3)

Targetcell

(1)

Non-targetcell

Cell surface antigen

Capture antibody Coiled-coil A

Coiled-coil B

PEG

Efficient Release of Affinity-Captured Cells Using Coiled-Coil-Based Molecular Triggers

Selective capture of endothelial cells

Efficient release of the captured endothelial cells by B-PEG

A label-free, affinity-based cell separation platformcomposed of a capture substrate and a cell-releasingmolecular trigger. The capture substrate is functionalizedwith a capture antibody and a coiled-coil A. The cell-releasing molecular trigger B-PEG, a conjugate of coiled-coil B and polyethylene glycol, can drive efficient andgentle release of the captured cells. No excessive shearstress or enzymes are involved, and the released cellshave neither external molecules attached nor endogenouscell-surface molecules cleaved, which might be critical forthe viability, phenotype, and function of sensitive cells.

Wei Shen, shenx104@umn.edu, 612-624-3771

Zhang, M. and Shen, W. , Macromolecular Bioscience, 2017, 17, 1600330

Spacer between single stranded DNA (ssDNA) and hydrophobic tail affects self-assembly, secondary structure and binding.

Depending on the spacer used ssDNA-amphiphiles self-assemble into sphericalmicelles and bilayer nanotapes. The nano-tapes progress from twisted nanotapes tohelical nanotapes to nanotubes.

We can control the diameter and length of thessDNA nanotubes, and we are exploring bothssDNA micelles and nanotubes for the targeteddelivery of oligonucleotides.

200 nmHelical

Tube

200 nm

Twisted

Helical

Self-Assembling DNA AmphiphilesEfie Kokkoli, Department of Chemical Engineering and Materials Science

kokko002@umn.edu, 612-626-1185http://research.cems.umn.edu/kokko002/home/

Pearce & Kokkoli, Soft Matter, 2015 11 (1): 109-117

Punch sticking – mechanism, kinetics, and mitigation strategies

http://www.pharmacy.umn.edu/faculty/sun_changquancalvin/index.htm

Paul et al., J. Pharm. Sci. , (2017), 106, 151-158Paul et al., Int. J. Pharm., (2017), 521, 374-383Paul et al., J. Pharm. Sci. , (2017), https://doi.org/10.1016/j.xphs.2017.04.059

Changquan Calvin Sun (email: sunx0053@umn.edu, Dept of Pharmaceutics Tel. 612-624-3722)

= excipient particle

= API particle

Powder bed

Type I

Type II

Punch tipPunch sticking

Surface area

Tablet tensile

strength

Ejection force

Take-off force