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REPLICATION OF DNA. Restriction enzyme. Endonuclease – cleave the nt in the middle of DNA molecule Exonuclease - cleave the nt from the end of DNA molecule. The flow of genetic information. Duplication of DNA to produce a new DNA molecule with the same base sequence as original - PowerPoint PPT Presentation
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REPLICATION OF DNA
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Restriction enzyme
• Endonuclease – cleave the nt in the middle of DNA molecule
• Exonuclease - cleave the nt from the end of DNA molecule
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The flow of genetic information
• Duplication of DNA to produce a new DNA molecule with the same base sequence as original
• A necessary process whenever a cell divides to produce daughter cells
• Flow of genetic information
DNA –RNA-Protein
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Replication process• A complex process
• Ensures fidelity
• 3 important challenge1. Separating the two strands – the double
helix must be unwound if they are to be separated. At the same time, the unwound portions must be protected from the action of nucleases that prefer to attack ss DNA.
2. Synthesizing DNA from 5’ end to the 3’end.
3. Guarding against error in replication – ensuring the correct base is added
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Types of replication• Conservative
The parental strands never completely separateAfter on round replication, one daughter duplex contains only parental strands and the other only daughter strands
• Semi conservativeThe process of unwinding, of the double helical daughter molecules – that is composed of a parental strand and a newly synthesized strand formed from the complementary strand
• Theta replication – replication in a circular form -prokaryote
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Semiconservative replication
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Steps involved in DNA replicationa) Identification of the origins of replicationb) Unwinding (denaturation) of dsDNA to provide ssDNA templatec) Formation of the replication forkd) Initiation of DNA synthesis and elongatione) Formation of replication bubbles with ligation of the newly
synthesized DNA segmentsf) Proof reading process
DNA replication in Prokaryote and eukaryote generally involve the same steps, except in eukaryote the process is more complex due to the presence of histone complex
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DNA REPLICATION IN E.COLI-FAMOUS MODEL
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a) Origin of replication
• Origin of replication (oriC )- spesific sites where replication starts
• Sequence specific DNA binding proteins (O Protein) will bind to ori
• Adjacent to ori is A+T region• Binding of O protein lead to local denaturation and unwinding
of A+T region
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b) Unwinding of DNA to form SS DNA which act as template
• Interaction of O protein provide a short region of ssDNA essential for initiation of replication
• This region – a template for initiation• DNA helicases helps in unwinding of DNA• DNA helices produces nicks in one strand of
double helix• Single stranded binding proteins (SSB
Proteins) – bind to SS each strand and stabilize the complex and prevents re-annealing
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DNA Replication Process
1. DNA helicase unwinds short segment of parent DNA2. SSB protein stabilize the unwound parental DNA3. A primase initiates synthesis of RNA molecule (primer) that is essential for
priming DNA synthesis4. Initiation of rep-require priming by short length of RNA (primer) (10 to 200nts)
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Template and primer
3’-OH 5’Template
5’ 3’-OHPrimerAGCTACTGCT…….
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DNA Replication Process• Priming process-nucleophilic attack by 3’-OH group of the RNA primer on the
α-phoshate of the first entering nucleotide with release of pyrophosphate
• Elongation-3’-OH of the newly attached nt is then free to carry out nucleophilic attack on the next entering nt
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DNA Replication Process
• DNA polymerase III begin replication by adding new complementary strand nt to the primer-producing polynucleotide chain
• DNA polymerase III cannot initiate DNA synthesis ‘de novo’• Leading strand (fwd strand)-the DNA is synthesized continuously in 5’to3’
direction with the same over-all fwd direction
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• Lagging strand – the DNA is synthesized in discontinuous manner-not directly as leading strand- DNA Pol III only add nt to 3-OH – So the system is reversed to comply to its function
• As replication fork is produced, a primer is synthesized from 5’ to 3’ and DNA Pol III begin the polymerization process –producing short DNA strand –Okazaki fragments
DNA Replication Process
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• Okazaki fragments are 100-250nt long in eukaryotes and 1000-2000bp in prokaryotes
• RNA primers will be removed by DNA Pol I (using its exonuclease activity)• Leaving primers leave a gap (at least one nt missing) - • The gap will be replaced by nt by DNA Pol I – leaving only a nick (interruption
in the phosphodiester bond with no missing nts)• These nicks are sealed by DNA ligases producing a long polynucleotide chains
DNA Replication Process
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Termination of replication (prokaryote)
• The 2 replication forks of E.Coli meet at terminus region (Ter)
• Terminal Utilization Protein (Tus) will bind to Ter – forming Tus-Tur Complex and stop the rep fork- completing 2 interlinked circular chromosome (catenated)
• DNA Topoisomerase IV separated the chromosome – segregate into daughter cells
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DNA REPLICATION IN EUKARYOTES
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DNA REPLICATION IN EUKARYOTE
• Mostly studied - yeast• The understanding is not as much as in
prokaryotes• More complicated– Multiple Ori- replicators (around 400 replicators in
yeast)– Timing must be controlled to that off cell division– More proteins and enzymes involved
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• Cell growth- M, G1, S and G2
• Gap 1 (G1) Cell prepare for DNA synthesis
• DNA replication occur in S phase – synthetic S phase
• Transition from one phase to another is controlled by cyclins proteins
• The DNA is replicated once and only once
DNA REPLICATION IN EUKARYOTE
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• Cancer-causing virus (oncoviruses) and cancer inducing genes (oncogenes) – capable of disrupting the entry of mammalian cells from G1 to S – excessive production of cyclin – production in an appropriate time – abnormal cell division
• Once a chromatin has been replicated, it is marked to further its replication until it again passes through mitosis
DNA REPLICATION IN EUKARYOTE
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The DNA Polymerase ComplexE.Coli Mammalian Function
I α Gap filling and synthesis of lagging strand
II ξ DNA Proof reading and repair
β DNA Repair
γ Mitochondrial DNA synthesis
III δ Processive, leading strand synthesis
In mammalian cell, the polymerase is capable of polymerizing about 100nt persecond – ten fold slower than in bacterial
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Replication bubble • In prokaryote- genome size is around 6x106 bp – replication is completed
in 30 mins (replication rate 3x105bp per min)
• In eukaryote – genome size is 3x109
Replicated in the same rate – will take 150 hours to complete!
Problem is overcome by having multiple origin in chromosome and replication occur in both direction along all of the chromosome
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Termination of replication in eukaryotes
• Require a special mechanism because the DNA is linear• Leading strand -5’ end template is not a problem,
because the DNA is synthesized from 5’to3’ (continuous process and require only one primer in the beginning.
• Lagging strand- problem
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Termination of replication in eukaryotes
Lagging strand3’ 5’
The last RNA Primer need to be removed, the gap need to be filled with DNA, but without RNA, the DNA cannot be synthesized
Lagging strand
5’
????????
The DNA can become shorter and shorter every time replication occur
TTAGGG
Telomerase added telomere repeats to the 3’end of the new strand-serve as primer
3’5’
5’
3’
3’
The last segment of DNA can be synthesized- complete the replication
5’
5’3’
3’
3’
3’
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REPLICATION ERRORS AND THEIR REPAIR
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Causes of DNA Damage
DNA damage during DNA replication can occur through:• Mis-incorporation of dntp
during replication• By spontaneous deamination
of bases during normal genetic functions
• From X- radiation that cause nicks in the DNA
• From various chemicals that interact with DNA
The rapid repair is needed as they may be lethal to
the cell
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Effect of replication errors
• Mutations – permanent changes- not fx
• Prevent it to be used as the template for replication and transcription
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Types of damages to DNA
• Single base alteration• Two base alteration• Chain breaks• Cross-linkage
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Cell cycle checkpoint
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Proof reading mechanism
• Error can occur once in 104 to 105 base pairs• Proof reading -Removal of incorrect nt immediately
after they are added to the growing DNA during the replication process
• Performed by DNA Pol I• DNA Pol I have two major components – – Klenow fragment (for polymerase & proofreading
activity)– 5’ to 3’ repair activity
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• Occur at the last stage of replication, after removing of primers -Through cut and patch process– Cut – removal the DNA mistakes as it moves along the
DNA– Patch – Fills in the right nt
Proof reading mechanism
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Some replication errors escapes proofreading
• Proof reading activity- increase the fidelity• However, there are some errors escape• These errors need to be minimized before it
become permanent damaged in the DNA sequence
• Performed through genome scanning by the protein MutS
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Mismatched repair1. The newly synthesized DNA
has a mismatched
2. MutH, Mut S, Mut L complex will bring the mismatch with the nearest methylation site – to identify the parent strand. The methylated strand is the parent strand
3. An exonuclease removes DNA from the red strand between proteins (damaged dna)
4. DNA Polymerase replace the removed DNA with the correct sequence
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Base excision repair• To repair base that is
damaged by oxidation or chemical modification
• The damaged base is removed by DNA glycosylase –leaving AP site
• AP endonuclease then removes s the sugar and PO4 from the group
• Excision nuclease removes several more bases
• DNA Pol I fill the gap
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Nucleotide excision repair
• For DNA damaged due to the UV or chemical effect – lead to deformed DNA structure
• ABC excinuclease remove the large section of damaged DNA
• DNA Pol I add new nt• DNA ligases seal the gap
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Eukaryotes
• Initiation point specific (ori)• DNA Polymerase- 3 types – I, II, III• DNA Pol I has diverse function• Not applicable• No repair function• Replication with few replication
forks• Theta structure observed• Accessory proteins few with limited
functions• Only unwinding takes place in
prokaryotes• Not at all or few replication
bubbles• RNA as primer
• Initiation point specific but different in prokaryotes
• DNA Pol – 5 types –αξβϒδ• Functional variety of DNA
polymerase is specific• γ DNA polymerase In mitochondria • Β- Polymerase functions as repair
enzyme• Many replication forks• Theta structure not observed• Many accessory proteins with
diverse functions• Histone separation from DNA as
well as unwinding takes place• Many replication bubbles• RNA/DNA as primer
Prokaryotes
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Summary• Identification of sites of the origin of replication (ori)• Unwinding of parental DNA (dsDNA ssDNA)• Formation of replication fork• Synthesis of RNA primer, complementary to DNA template, the
enzyme required is primase• Leading strand is synthesized in the 5’to 3’ direction by the
enzyme DNA polymerase• Lagging strand is synthesized as Okazaki fragments• RNA pieces are removed when polymerization is complete • The gaps are filled by nt and the pieces are joined by DNA
ligases