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Relationship between radiation dose and chromosome aberration frequency – Calibration curves. Piotr Knapp Piotr Wróblewski. Supervisors: Ekaterina Zaytseva, Polina Kutsalo, Marta Deperas-Kamińska. Laboratory of Radiation Biology. The fate of irradiated cell. Gy. After high dose. - PowerPoint PPT Presentation
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Piotr KnappPiotr Wróblewski
Laboratory of Radiation Biology
Supervisors: Ekaterina Zaytseva, Polina Kutsalo, Marta Deperas-Kamińska
The fate of irradiated cell
Necroticdeath
After high dose
Somaticeffect
Healthy cell
Noeffect
Micronuclei
Mitotic death
No effect
Cancer
Gy
Somaticeffect
Geneticeffect
Carcinogeniceffect
Somaticeffect
Cell with damaged DNA
Correct repair
Incorrect repair
Unstable chromosomalaberrations
Apoptotic death
Stable chromosomalAberrations - Mutations
In the germ cells
Mutation path
Creation of dose dependency calibration curve of chromosomal aberrations in human peripherial blood lymphocytes exposed to protons beam (LET≤100keV/µm) performed at Phasotrone (LNP, JINR)
Biological dosimetry - Allow to assess an accidental radiation dose on base of the level of changes in chromosome structure.
Culture of irradiated
blood
BrdU
0-24h
Colcemid
48h
Fixation
48-50h
Watching
Staining with Fluorescence Plus
Giemsa
An example of cell stained with Giemsa dye with dicentric
chromosome.
DOSE [Gy]
Aberration per cell
Marta Deperas-Kamińska
Polina Kutsalo
Ekaterina Zaytseva